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Sequence NM_198436
ACAAGGCAGCCTCGCTCGAGCGCAGGCCAATCGGCTTTCTAGCTAGAGGGTTTAACTCCTATTTAAAAAGAAGAACCTTTGAATTCTAACGGCTGAGCTCTTGGAAGACTTGGGTCCTTGGGTCGCAGGGTCTCACTCCATTGCCCAGGCCAGAGTGCGGGGATATTTGATAAGAAACTTCAGTGAAGGCCGGGCGCGGT
GGCTCATGCCCGTAATCCCAGCATTTTCGGAGGCCGAGGCATCATGGACCGATCTAAAGAAAACTGCATTTCAGGACCTGTTAAGGCTACAGCTCCAGTT
GGAGGTCCAAAACGTGTTCTCGTGACTCAGCAATTTCCTTGTCAGAATCCATTACCTGTAAATAGTGGCCAGGCTCAGCGGGTCTTGTGTCCTTCAAATT
CTTCCCAGCGCATTCCTTTGCAAGCACAAAAGCTTGTCTCCAGTCACAAGCCGGTTCAGAATCAGAAGCAGAAGCAATTGCAGGCAACCAGTGTACCTCA
TCCTGTCTCCAGGCCACTGAATAACACCCAAAAGAGCAAGCAGCCCCTGCCATCGGCACCTGAAAATAATCCTGAGGAGGAACTGGCATCAAAACAGAAA
AATGAAGAATCAAAAAAGAGGCAGTGGGCTTTGGAAGACTTTGAAATTGGTCGCCCTCTGGGTAAAGGAAAGTTTGGTAATGTTTATTTGGCAAGAGAAA
AGCAAAGCAAGTTTATTCTGGCTCTTAAAGTGTTATTTAAAGCTCAGCTGGAGAAAGCCGGAGTGGAGCATCAGCTCAGAAGAGAAGTAGAAATACAGTC
CCACCTTCGGCATCCTAATATTCTTAGACTGTATGGTTATTTCCATGATGCTACCAGAGTCTACCTAATTCTGGAATATGCACCACTTGGAACAGTTTAT
AGAGAACTTCAGAAACTTTCAAAGTTTGATGAGCAGAGAACTGCTACTTATATAACAGAATTGGCAAATGCCCTGTCTTACTGTCATTCGAAGAGAGTTA
TTCATAGAGACATTAAGCCAGAGAACTTACTTCTTGGATCAGCTGGAGAGCTTAAAATTGCAGATTTTGGGTGGTCAGTACATGCTCCATCTTCCAGGAG
GACCACTCTCTGTGGCACCCTGGACTACCTGCCCCCTGAAATGATTGAAGGTCGGATGCATGATGAGAAGGTGGATCTCTGGAGCCTTGGAGTTCTTTGC
TATGAATTTTTAGTTGGGAAGCCTCCTTTTGAGGCAAACACATACCAAGAGACCTACAAAAGAATATCACGGGTTGAATTCACATTCCCTGACTTTGTAA
CAGAGGGAGCCAGGGACCTCATTTCAAGACTGTTGAAGCATAATCCCAGCCAGAGGCCAATGCTCAGAGAAGTACTTGAACACCCCTGGATCACAGCAAA
TTCATCAAAACCATCAAATTGCCAAAACAAAGAATCAGCTAGCAAACAGTCTTAGGAATCGTGCAGGGGGAGAAATCCTTGAGCCAGGGCTGCCATATAA
CCTGACAGGAACATGCTACTGAAGTTTATTTTACCATTGACTGCTGCCCTCAATCTAGAACGCTACACAAGAAATATTTGTTTTACTCAGCAGGTGTGCC
TTAACCTCCCTATTCAGAAAGCTCCACATCAATAAACATGACACTCTGAAGTGAAAGTAGCCACGAGAATTGTGCTACTTATACTGGTTCATAATCTGGA
GGCAAGGTTCGACTGCAGCCGCCCCGTCAGCCTGTGCTAGGCATGGTGTCTTCACAGGAGGCAAATCCAGAGCCTGGCTGTGGGGAAAGTGACCACTCTG
CCCTGACCCCGATCAGTTAAGGAGCTGTGCAATAACCTTCCTAGTACCTGAGTGAGTGTGTAACTTATTGGGTTGGCGAAGCCTGGTAAAGCTGTTGGAA
TGAGTATGTGATTCTTTTTAAGTATGAAAATAAAGATATATGTACAGACTTGTATTTTTTCTCTGGTGGCATTCCTTTAGGAATGCTGTGTGTCTGTCCG
GCACCCCGGTAGGCCTGATTGGGTTTCTAGTCCTCCTTAACCACTTATCTCCCATATGAGAGTGTGAAAAATAGGAACACGTGCTCTACCTCCATTTAGG
GATTTGCTTGGGATACAGAAGAGGCCATGTGTCTCAGAGCTGTTAAGGGCTTATTTTTTTAAAACATTGGAGTCATAGCATGTGTGTAAACTTTAAATAT
GCAAATAAATAAGTATCTATGTCT
- refseq_AURKA.F3 (forward primer)
- refseq_AURKA.R3 (reverse primer)
- Alternative donor
Frame 0
TRQPRSSAGQSAF*LEGLTPI*KEEPLNSNG*ALGRLGSLGRRVSLHCPGQSAGIFDKKLQ*RPGAV
AHARNPSIFGGRGIMDRSKENCISGPVKATAPV
GGPKRVLVTQQFPCQNPLPVNSGQAQRVLCPSN
SSQRIPLQAQKLVSSHKPVQNQKQKQLQATSVPH
PVSRPLNNTQKSKQPLPSAPENNPEEELASKQK
NEESKKRQWALEDFEIGRPLGKGKFGNVYLARE
KQSKFILALKVLFKAQLEKAGVEHQLRREVEIQS
HLRHPNILRLYGYFHDATRVYLILEYAPLGTVY
RELQKLSKFDEQRTATYITELANALSYCHSKRV
IHRDIKPENLLLGSAGELKIADFGWSVHAPSSRR
TTLCGTLDYLPPEMIEGRMHDEKVDLWSLGVLC
YEFLVGKPPFEANTYQETYKRISRVEFTFPDFV
TEGARDLISRLLKHNPSQRPMLREVLEHPWITAN
SSKPSNCQNKESASKQS*ESCRGRNP*ARAAI*
PDRNMLLKFILPLTAALNLERYTRNICFTQQVC
LNLPIQKAPHQ*T*HSEVKVATRIVLLILVHNLE
ARFDCSRPVSLC*AWCLHRRQIQSLAVGKVTTL
P*PRSVKELCNNLPST*VSV*LIGLAKPGKAVG
MSM*FFLSMKIKIYVQTCIFSLVAFL*ECCVSVR
HPGRPDWVSSPP*PLISHMRV*KIGTRALPPFR
DLLGIQKRPCVSELLRAYFFKTLES*HVCKL*I
CK*ISIYV
Frame 1
QGSLARAQANRLSS*RV*LLFKKKNL*ILTAELLEDLGPWVAGSHSIAQARVRGYLIRNFSEGRAR
WLMPVIPAFSEAEASWTDLKKTAFQDLLRLQLQL
EVQNVFS*LSNFLVRIHYL*IVARLSGSCVLQI
LPSAFLCKHKSLSPVTSRFRIRSRSNCRQPVYL
ILSPGH*ITPKRASSPCHRHLKIILRRNWHQNRK
MKNQKRGSGLWKTLKLVALWVKESLVMFIWQEK
SKASLFWLLKCYLKLSWRKPEWSISSEEK*KYS
PTFGILIFLDCMVISMMLPEST*FWNMHHLEQFI
ENFRNFQSLMSRELLLI*QNWQMPCLTVIRREL
FIETLSQRTYFLDQLESLKLQILGGQYMLHLPG
GPLSVAPWTTCPLK*LKVGCMMRRWISGALEFFA
MNF*LGSLLLRQTHTKRPTKEYHGLNSHSLTL*
QREPGTSFQDC*SIIPARGQCSEKYLNTPGSQQ
IHQNHQIAKTKNQLANSLRNRAGGEILEPGLPYN
LTGTCY*SLFYH*LLPSI*NATQEIFVLLSRCA
LTSLFRKLHINKHDTLK*K*PRELCYLYWFIIW
RQGSTAAAPSACARHGVFTGGKSRAWLWGK*PLC
PDPDQLRSCAITFLVPE*VCNLLGWRSLVKLLE
*VCDSF*V*K*RYMYRLVFFLWWHSFRNAVCLS
GTPVGLIGFLVLLNHLSPI*ECEK*EHVLYLHLG
ICLGYRRGHVSQSC*GLIFLKHWSHSMCVNFKY
ANK*VSMS
Frame 2
KAASLERRPIGFLARGFNSYLKRRTFEF*RLSSWKTWVLGSQGLTPLPRPECGDI**ETSVKAGRG
GSCP*SQHFRRPRHHGPI*RKLHFRTC*GYSSS
WRSKTCSRDSAISLSESITCK*WPGSAGLVSFKF
FPAHSFASTKACLQSQAGSESEAEAIAGNQCTS
SCLQATE*HPKEQAAPAIGT*K*S*GGTGIKTE
K*RIKKEAVGFGRL*NWSPSG*RKVW*CLFGKRK
AKQVYSGS*SVI*SSAGESRSGASAQKRSRNTV
PPSAS*YS*TVWLFP*CYQSLPNSGICTTWNSL
*RTSETFKV**AENCYLYNRIGKCPVLLSFEESY
S*RH*ARELTSWISWRA*NCRFWVVSTCSIFQE
DHSLWHPGLPAP*ND*RSDA**EGGSLEPWSSL
L*IFSWEASF*GKHIPRDLQKNITG*IHIP*LCN
RGSQGPHFKTVEA*SQPEANAQRST*TPLDHSK
FIKTIKLPKQRIS*QTVLGIVQGEKSLSQGCHI
T*QEHATEVYFTIDCCPQSRTLHKKYLFYSAGVP
*PPYSESSTSINMTL*SESSHENCATYTGS*SG
GKVRLQPPRQPVLGMVSSQEANPEPGCGESDHS
ALTPIS*GAVQ*PS*YLSECVTYWVGEAW*SCWN
EYVILFKYENKDICTDLYFFSGGIPLGMLCVCP
APR*A*LGF*SSLTTYLPYESVKNRNTCSTSI*
GFAWDTEEAMCLRAVKGLFF*NIGVIACV*TLNM
QINKYLC
Sequence NM_198434
ACAAGGCAGCCTCGCTCGAGCGCAGGCCAATCGGCTTTCTAGCTAGAGGGTTTAACTCCTATTTAAAAAGAAGAACCTTTGAATTCTAACGGCTGAGCTCTTGGAAGACTTGGGTCCTTGGGTCGCAGGTGGGAGCCGACGGGGTCTCACTCCATTGCCCAGGCCAGAGTGCGGGGATATTTGATAAGAAACTTCAGTGA
AGGCCGGGCGCGGTGGCTCATGCCCGTAATCCCAGCATTTTCGGAGGCCGAGGCATCATGGACCGATCTAAAGAAAACTGCATTTCAGGACCTGTTAAGG
CTACAGCTCCAGTTGGAGGTCCAAAACGTGTTCTCGTGACTCAGCAATTTCCTTGTCAGAATCCATTACCTGTAAATAGTGGCCAGGCTCAGCGGGTCTT
GTGTCCTTCAAATTCTTCCCAGCGCATTCCTTTGCAAGCACAAAAGCTTGTCTCCAGTCACAAGCCGGTTCAGAATCAGAAGCAGAAGCAATTGCAGGCA
ACCAGTGTACCTCATCCTGTCTCCAGGCCACTGAATAACACCCAAAAGAGCAAGCAGCCCCTGCCATCGGCACCTGAAAATAATCCTGAGGAGGAACTGG
CATCAAAACAGAAAAATGAAGAATCAAAAAAGAGGCAGTGGGCTTTGGAAGACTTTGAAATTGGTCGCCCTCTGGGTAAAGGAAAGTTTGGTAATGTTTA
TTTGGCAAGAGAAAAGCAAAGCAAGTTTATTCTGGCTCTTAAAGTGTTATTTAAAGCTCAGCTGGAGAAAGCCGGAGTGGAGCATCAGCTCAGAAGAGAA
GTAGAAATACAGTCCCACCTTCGGCATCCTAATATTCTTAGACTGTATGGTTATTTCCATGATGCTACCAGAGTCTACCTAATTCTGGAATATGCACCAC
TTGGAACAGTTTATAGAGAACTTCAGAAACTTTCAAAGTTTGATGAGCAGAGAACTGCTACTTATATAACAGAATTGGCAAATGCCCTGTCTTACTGTCA
TTCGAAGAGAGTTATTCATAGAGACATTAAGCCAGAGAACTTACTTCTTGGATCAGCTGGAGAGCTTAAAATTGCAGATTTTGGGTGGTCAGTACATGCT
CCATCTTCCAGGAGGACCACTCTCTGTGGCACCCTGGACTACCTGCCCCCTGAAATGATTGAAGGTCGGATGCATGATGAGAAGGTGGATCTCTGGAGCC
TTGGAGTTCTTTGCTATGAATTTTTAGTTGGGAAGCCTCCTTTTGAGGCAAACACATACCAAGAGACCTACAAAAGAATATCACGGGTTGAATTCACATT
CCCTGACTTTGTAACAGAGGGAGCCAGGGACCTCATTTCAAGACTGTTGAAGCATAATCCCAGCCAGAGGCCAATGCTCAGAGAAGTACTTGAACACCCC
TGGATCACAGCAAATTCATCAAAACCATCAAATTGCCAAAACAAAGAATCAGCTAGCAAACAGTCTTAGGAATCGTGCAGGGGGAGAAATCCTTGAGCCA
GGGCTGCCATATAACCTGACAGGAACATGCTACTGAAGTTTATTTTACCATTGACTGCTGCCCTCAATCTAGAACGCTACACAAGAAATATTTGTTTTAC
TCAGCAGGTGTGCCTTAACCTCCCTATTCAGAAAGCTCCACATCAATAAACATGACACTCTGAAGTGAAAGTAGCCACGAGAATTGTGCTACTTATACTG
GTTCATAATCTGGAGGCAAGGTTCGACTGCAGCCGCCCCGTCAGCCTGTGCTAGGCATGGTGTCTTCACAGGAGGCAAATCCAGAGCCTGGCTGTGGGGA
AAGTGACCACTCTGCCCTGACCCCGATCAGTTAAGGAGCTGTGCAATAACCTTCCTAGTACCTGAGTGAGTGTGTAACTTATTGGGTTGGCGAAGCCTGG
TAAAGCTGTTGGAATGAGTATGTGATTCTTTTTAAGTATGAAAATAAAGATATATGTACAGACTTGTATTTTTTCTCTGGTGGCATTCCTTTAGGAATGC
TGTGTGTCTGTCCGGCACCCCGGTAGGCCTGATTGGGTTTCTAGTCCTCCTTAACCACTTATCTCCCATATGAGAGTGTGAAAAATAGGAACACGTGCTC
TACCTCCATTTAGGGATTTGCTTGGGATACAGAAGAGGCCATGTGTCTCAGAGCTGTTAAGGGCTTATTTTTTTAAAACATTGGAGTCATAGCATGTGTG
TAAACTTTAAATATGCAAATAAATAAGTATCTATGTCT
- refseq_AURKA.F3 (forward primer)
- refseq_AURKA.R3 (reverse primer)
- Alternative donor
Frame 0
TRQPRSSAGQSAF*LEGLTPI*KEEPLNSNG*ALGRLGSLGRRWEPTGSHSIAQARVRGYLIRNFSE
GRARWLMPVIPAFSEAEASWTDLKKTAFQDLLR
LQLQLEVQNVFS*LSNFLVRIHYL*IVARLSGS
CVLQILPSAFLCKHKSLSPVTSRFRIRSRSNCRQ
PVYLILSPGH*ITPKRASSPCHRHLKIILRRNW
HQNRKMKNQKRGSGLWKTLKLVALWVKESLVMF
IWQEKSKASLFWLLKCYLKLSWRKPEWSISSEEK
*KYSPTFGILIFLDCMVISMMLPEST*FWNMHH
LEQFIENFRNFQSLMSRELLLI*QNWQMPCLTV
IRRELFIETLSQRTYFLDQLESLKLQILGGQYML
HLPGGPLSVAPWTTCPLK*LKVGCMMRRWISGA
LEFFAMNF*LGSLLLRQTHTKRPTKEYHGLNSH
SLTL*QREPGTSFQDC*SIIPARGQCSEKYLNTP
GSQQIHQNHQIAKTKNQLANSLRNRAGGEILEP
GLPYNLTGTCY*SLFYH*LLPSI*NATQEIFVL
LSRCALTSLFRKLHINKHDTLK*K*PRELCYLYW
FIIWRQGSTAAAPSACARHGVFTGGKSRAWLWG
K*PLCPDPDQLRSCAITFLVPE*VCNLLGWRSL
VKLLE*VCDSF*V*K*RYMYRLVFFLWWHSFRNA
VCLSGTPVGLIGFLVLLNHLSPI*ECEK*EHVL
YLHLGICLGYRRGHVSQSC*GLIFLKHWSHSMC
VNFKYANK*VSMS
Frame 1
QGSLARAQANRLSS*RV*LLFKKKNL*ILTAELLEDLGPWVAGGSRRGLTPLPRPECGDI**ETSV
KAGRGGSCP*SQHFRRPRHHGPI*RKLHFRTC*G
YSSSWRSKTCSRDSAISLSESITCK*WPGSAGL
VSFKFFPAHSFASTKACLQSQAGSESEAEAIAG
NQCTSSCLQATE*HPKEQAAPAIGT*K*S*GGTG
IKTEK*RIKKEAVGFGRL*NWSPSG*RKVW*CL
FGKRKAKQVYSGS*SVI*SSAGESRSGASAQKR
SRNTVPPSAS*YS*TVWLFP*CYQSLPNSGICTT
WNSL*RTSETFKV**AENCYLYNRIGKCPVLLS
FEESYS*RH*ARELTSWISWRA*NCRFWVVSTC
SIFQEDHSLWHPGLPAP*ND*RSDA**EGGSLEP
WSSLL*IFSWEASF*GKHIPRDLQKNITG*IHI
P*LCNRGSQGPHFKTVEA*SQPEANAQRST*TP
LDHSKFIKTIKLPKQRIS*QTVLGIVQGEKSLSQ
GCHIT*QEHATEVYFTIDCCPQSRTLHKKYLFY
SAGVP*PPYSESSTSINMTL*SESSHENCATYT
GS*SGGKVRLQPPRQPVLGMVSSQEANPEPGCGE
SDHSALTPIS*GAVQ*PS*YLSECVTYWVGEAW
*SCWNEYVILFKYENKDICTDLYFFSGGIPLGM
LCVCPAPR*A*LGF*SSLTTYLPYESVKNRNTCS
TSI*GFAWDTEEAMCLRAVKGLFF*NIGVIACV
*TLNMQINKYLC
Frame 2
KAASLERRPIGFLARGFNSYLKRRTFEF*RLSSWKTWVLGSQVGADGVSLHCPGQSAGIFDKKLQ*
RPGAVAHARNPSIFGGRGIMDRSKENCISGPVK
ATAPVGGPKRVLVTQQFPCQNPLPVNSGQAQRVL
CPSNSSQRIPLQAQKLVSSHKPVQNQKQKQLQA
TSVPHPVSRPLNNTQKSKQPLPSAPENNPEEEL
ASKQKNEESKKRQWALEDFEIGRPLGKGKFGNVY
LAREKQSKFILALKVLFKAQLEKAGVEHQLRRE
VEIQSHLRHPNILRLYGYFHDATRVYLILEYAP
LGTVYRELQKLSKFDEQRTATYITELANALSYCH
SKRVIHRDIKPENLLLGSAGELKIADFGWSVHA
PSSRRTTLCGTLDYLPPEMIEGRMHDEKVDLWS
LGVLCYEFLVGKPPFEANTYQETYKRISRVEFTF
PDFVTEGARDLISRLLKHNPSQRPMLREVLEHP
WITANSSKPSNCQNKESASKQS*ESCRGRNP*A
RAAI*PDRNMLLKFILPLTAALNLERYTRNICFT
QQVCLNLPIQKAPHQ*T*HSEVKVATRIVLLIL
VHNLEARFDCSRPVSLC*AWCLHRRQIQSLAVG
KVTTLP*PRSVKELCNNLPST*VSV*LIGLAKPG
KAVGMSM*FFLSMKIKIYVQTCIFSLVAFL*EC
CVSVRHPGRPDWVSSPP*PLISHMRV*KIGTRA
LPPFRDLLGIQKRPCVSELLRAYFFKTLES*HVC
KL*ICK*ISIYV
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