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Sequence NM_198436
ACAAGGCAGCCTCGCTCGAGCGCAGGCCAATCGGCTTTCTAGCTAGAGGGTTTAACTCCTATTTAAAAAGAAGAACCTTTGAATTCTAACGGCTGAGCTCTTGGAAGACTTGGGTCCTTGGGTCGCAGGGTCTCACTCCATTGCCCAGGCCAGAGTGCGGGGATATTTGATAAGAAACTTCAGTGAAGGCCGGGCGCGGT
GGCTCATGCCCGTAATCCCAGCATTTTCGGAGGCCGAGGCATCATGGACCGATCTAAAGAAAACTGCATTTCAGGACCTGTTAAGGCTACAGCTCCAGTT
GGAGGTCCAAAACGTGTTCTCGTGACTCAGCAATTTCCTTGTCAGAATCCATTACCTGTAAATAGTGGCCAGGCTCAGCGGGTCTTGTGTCCTTCAAATT
CTTCCCAGCGCATTCCTTTGCAAGCACAAAAGCTTGTCTCCAGTCACAAGCCGGTTCAGAATCAGAAGCAGAAGCAATTGCAGGCAACCAGTGTACCTCA
TCCTGTCTCCAGGCCACTGAATAACACCCAAAAGAGCAAGCAGCCCCTGCCATCGGCACCTGAAAATAATCCTGAGGAGGAACTGGCATCAAAACAGAAA
AATGAAGAATCAAAAAAGAGGCAGTGGGCTTTGGAAGACTTTGAAATTGGTCGCCCTCTGGGTAAAGGAAAGTTTGGTAATGTTTATTTGGCAAGAGAAA
AGCAAAGCAAGTTTATTCTGGCTCTTAAAGTGTTATTTAAAGCTCAGCTGGAGAAAGCCGGAGTGGAGCATCAGCTCAGAAGAGAAGTAGAAATACAGTC
CCACCTTCGGCATCCTAATATTCTTAGACTGTATGGTTATTTCCATGATGCTACCAGAGTCTACCTAATTCTGGAATATGCACCACTTGGAACAGTTTAT
AGAGAACTTCAGAAACTTTCAAAGTTTGATGAGCAGAGAACTGCTACTTATATAACAGAATTGGCAAATGCCCTGTCTTACTGTCATTCGAAGAGAGTTA
TTCATAGAGACATTAAGCCAGAGAACTTACTTCTTGGATCAGCTGGAGAGCTTAAAATTGCAGATTTTGGGTGGTCAGTACATGCTCCATCTTCCAGGAG
GACCACTCTCTGTGGCACCCTGGACTACCTGCCCCCTGAAATGATTGAAGGTCGGATGCATGATGAGAAGGTGGATCTCTGGAGCCTTGGAGTTCTTTGC
TATGAATTTTTAGTTGGGAAGCCTCCTTTTGAGGCAAACACATACCAAGAGACCTACAAAAGAATATCACGGGTTGAATTCACATTCCCTGACTTTGTAA
CAGAGGGAGCCAGGGACCTCATTTCAAGACTGTTGAAGCATAATCCCAGCCAGAGGCCAATGCTCAGAGAAGTACTTGAACACCCCTGGATCACAGCAAA
TTCATCAAAACCATCAAATTGCCAAAACAAAGAATCAGCTAGCAAACAGTCTTAGGAATCGTGCAGGGGGAGAAATCCTTGAGCCAGGGCTGCCATATAA
CCTGACAGGAACATGCTACTGAAGTTTATTTTACCATTGACTGCTGCCCTCAATCTAGAACGCTACACAAGAAATATTTGTTTTACTCAGCAGGTGTGCC
TTAACCTCCCTATTCAGAAAGCTCCACATCAATAAACATGACACTCTGAAGTGAAAGTAGCCACGAGAATTGTGCTACTTATACTGGTTCATAATCTGGA
GGCAAGGTTCGACTGCAGCCGCCCCGTCAGCCTGTGCTAGGCATGGTGTCTTCACAGGAGGCAAATCCAGAGCCTGGCTGTGGGGAAAGTGACCACTCTG
CCCTGACCCCGATCAGTTAAGGAGCTGTGCAATAACCTTCCTAGTACCTGAGTGAGTGTGTAACTTATTGGGTTGGCGAAGCCTGGTAAAGCTGTTGGAA
TGAGTATGTGATTCTTTTTAAGTATGAAAATAAAGATATATGTACAGACTTGTATTTTTTCTCTGGTGGCATTCCTTTAGGAATGCTGTGTGTCTGTCCG
GCACCCCGGTAGGCCTGATTGGGTTTCTAGTCCTCCTTAACCACTTATCTCCCATATGAGAGTGTGAAAAATAGGAACACGTGCTCTACCTCCATTTAGG
GATTTGCTTGGGATACAGAAGAGGCCATGTGTCTCAGAGCTGTTAAGGGCTTATTTTTTTAAAACATTGGAGTCATAGCATGTGTGTAAACTTTAAATAT
GCAAATAAATAAGTATCTATGTCT
- refseq_AURKA.F3 (forward primer)
- refseq_AURKA.R3 (reverse primer)
- Alternative donor
Frame 0
TRQPRSSAGQSAF*LEGLTPI*KEEPLNSNG*ALGRLGSLGRRVSLHCPGQSAGIFDKKLQ*RPGAV
AHARNPSIFGGRGIMDRSKENCISGPVKATAPV
GGPKRVLVTQQFPCQNPLPVNSGQAQRVLCPSN
SSQRIPLQAQKLVSSHKPVQNQKQKQLQATSVPH
PVSRPLNNTQKSKQPLPSAPENNPEEELASKQK
NEESKKRQWALEDFEIGRPLGKGKFGNVYLARE
KQSKFILALKVLFKAQLEKAGVEHQLRREVEIQS
HLRHPNILRLYGYFHDATRVYLILEYAPLGTVY
RELQKLSKFDEQRTATYITELANALSYCHSKRV
IHRDIKPENLLLGSAGELKIADFGWSVHAPSSRR
TTLCGTLDYLPPEMIEGRMHDEKVDLWSLGVLC
YEFLVGKPPFEANTYQETYKRISRVEFTFPDFV
TEGARDLISRLLKHNPSQRPMLREVLEHPWITAN
SSKPSNCQNKESASKQS*ESCRGRNP*ARAAI*
PDRNMLLKFILPLTAALNLERYTRNICFTQQVC
LNLPIQKAPHQ*T*HSEVKVATRIVLLILVHNLE
ARFDCSRPVSLC*AWCLHRRQIQSLAVGKVTTL
P*PRSVKELCNNLPST*VSV*LIGLAKPGKAVG
MSM*FFLSMKIKIYVQTCIFSLVAFL*ECCVSVR
HPGRPDWVSSPP*PLISHMRV*KIGTRALPPFR
DLLGIQKRPCVSELLRAYFFKTLES*HVCKL*I
CK*ISIYV
Frame 1
QGSLARAQANRLSS*RV*LLFKKKNL*ILTAELLEDLGPWVAGSHSIAQARVRGYLIRNFSEGRAR
WLMPVIPAFSEAEASWTDLKKTAFQDLLRLQLQL
EVQNVFS*LSNFLVRIHYL*IVARLSGSCVLQI
LPSAFLCKHKSLSPVTSRFRIRSRSNCRQPVYL
ILSPGH*ITPKRASSPCHRHLKIILRRNWHQNRK
MKNQKRGSGLWKTLKLVALWVKESLVMFIWQEK
SKASLFWLLKCYLKLSWRKPEWSISSEEK*KYS
PTFGILIFLDCMVISMMLPEST*FWNMHHLEQFI
ENFRNFQSLMSRELLLI*QNWQMPCLTVIRREL
FIETLSQRTYFLDQLESLKLQILGGQYMLHLPG
GPLSVAPWTTCPLK*LKVGCMMRRWISGALEFFA
MNF*LGSLLLRQTHTKRPTKEYHGLNSHSLTL*
QREPGTSFQDC*SIIPARGQCSEKYLNTPGSQQ
IHQNHQIAKTKNQLANSLRNRAGGEILEPGLPYN
LTGTCY*SLFYH*LLPSI*NATQEIFVLLSRCA
LTSLFRKLHINKHDTLK*K*PRELCYLYWFIIW
RQGSTAAAPSACARHGVFTGGKSRAWLWGK*PLC
PDPDQLRSCAITFLVPE*VCNLLGWRSLVKLLE
*VCDSF*V*K*RYMYRLVFFLWWHSFRNAVCLS
GTPVGLIGFLVLLNHLSPI*ECEK*EHVLYLHLG
ICLGYRRGHVSQSC*GLIFLKHWSHSMCVNFKY
ANK*VSMS
Frame 2
KAASLERRPIGFLARGFNSYLKRRTFEF*RLSSWKTWVLGSQGLTPLPRPECGDI**ETSVKAGRG
GSCP*SQHFRRPRHHGPI*RKLHFRTC*GYSSS
WRSKTCSRDSAISLSESITCK*WPGSAGLVSFKF
FPAHSFASTKACLQSQAGSESEAEAIAGNQCTS
SCLQATE*HPKEQAAPAIGT*K*S*GGTGIKTE
K*RIKKEAVGFGRL*NWSPSG*RKVW*CLFGKRK
AKQVYSGS*SVI*SSAGESRSGASAQKRSRNTV
PPSAS*YS*TVWLFP*CYQSLPNSGICTTWNSL
*RTSETFKV**AENCYLYNRIGKCPVLLSFEESY
S*RH*ARELTSWISWRA*NCRFWVVSTCSIFQE
DHSLWHPGLPAP*ND*RSDA**EGGSLEPWSSL
L*IFSWEASF*GKHIPRDLQKNITG*IHIP*LCN
RGSQGPHFKTVEA*SQPEANAQRST*TPLDHSK
FIKTIKLPKQRIS*QTVLGIVQGEKSLSQGCHI
T*QEHATEVYFTIDCCPQSRTLHKKYLFYSAGVP
*PPYSESSTSINMTL*SESSHENCATYTGS*SG
GKVRLQPPRQPVLGMVSSQEANPEPGCGESDHS
ALTPIS*GAVQ*PS*YLSECVTYWVGEAW*SCWN
EYVILFKYENKDICTDLYFFSGGIPLGMLCVCP
APR*A*LGF*SSLTTYLPYESVKNRNTCSTSI*
GFAWDTEEAMCLRAVKGLFF*NIGVIACV*TLNM
QINKYLC
Sequence NM_198433
ACAAGGCAGCCTCGCTCGAGCGCAGGCCAATCGGCTTTCTAGCTAGAGGGTTTAACTCCTATTTAAAAAGAAGAACCTTTGAATTCTAACGGCTGAGCTCTTGGAAGACTTGGGTCCTTGGGTCGCAGGTGGGAGCCGACGGGTGGGTAGACCGTGGGGGATATCTCAGTGGCGGACGAGGACGGCGGGGACAAGGGGCG
GCTGGTCGGAGTGGCGGAGCGTCAAGTCCCCTGTCGGTTCCTCCGTCCCTGAGTGTCCTTGGCGCTGCCTTGTGCCCGCCCAGCGCCTTTGCATCCGCTC
CTGGGCACCGAGGCGCCCTGTAGGATACTGCTTGTTACTTATTACAGCTAGAGGGTCTCACTCCATTGCCCAGGCCAGAGTGCGGGGATATTTGATAAGA
AACTTCAGTGAAGGCCGGGCGCGGTGGCTCATGCCCGTAATCCCAGCATTTTCGGAGGCCGAGGCTGGAGTGCAATGGTGTGATCTCAGCTCACTGCAAC
CTCTGCTTCCTGGGTTTAAGTGATTCTCCTGCCTCAGCCTCCCGAGTAGCTGGGATTACAGGCATCATGGACCGATCTAAAGAAAACTGCATTTCAGGAC
CTGTTAAGGCTACAGCTCCAGTTGGAGGTCCAAAACGTGTTCTCGTGACTCAGCAATTTCCTTGTCAGAATCCATTACCTGTAAATAGTGGCCAGGCTCA
GCGGGTCTTGTGTCCTTCAAATTCTTCCCAGCGCATTCCTTTGCAAGCACAAAAGCTTGTCTCCAGTCACAAGCCGGTTCAGAATCAGAAGCAGAAGCAA
TTGCAGGCAACCAGTGTACCTCATCCTGTCTCCAGGCCACTGAATAACACCCAAAAGAGCAAGCAGCCCCTGCCATCGGCACCTGAAAATAATCCTGAGG
AGGAACTGGCATCAAAACAGAAAAATGAAGAATCAAAAAAGAGGCAGTGGGCTTTGGAAGACTTTGAAATTGGTCGCCCTCTGGGTAAAGGAAAGTTTGG
TAATGTTTATTTGGCAAGAGAAAAGCAAAGCAAGTTTATTCTGGCTCTTAAAGTGTTATTTAAAGCTCAGCTGGAGAAAGCCGGAGTGGAGCATCAGCTC
AGAAGAGAAGTAGAAATACAGTCCCACCTTCGGCATCCTAATATTCTTAGACTGTATGGTTATTTCCATGATGCTACCAGAGTCTACCTAATTCTGGAAT
ATGCACCACTTGGAACAGTTTATAGAGAACTTCAGAAACTTTCAAAGTTTGATGAGCAGAGAACTGCTACTTATATAACAGAATTGGCAAATGCCCTGTC
TTACTGTCATTCGAAGAGAGTTATTCATAGAGACATTAAGCCAGAGAACTTACTTCTTGGATCAGCTGGAGAGCTTAAAATTGCAGATTTTGGGTGGTCA
GTACATGCTCCATCTTCCAGGAGGACCACTCTCTGTGGCACCCTGGACTACCTGCCCCCTGAAATGATTGAAGGTCGGATGCATGATGAGAAGGTGGATC
TCTGGAGCCTTGGAGTTCTTTGCTATGAATTTTTAGTTGGGAAGCCTCCTTTTGAGGCAAACACATACCAAGAGACCTACAAAAGAATATCACGGGTTGA
ATTCACATTCCCTGACTTTGTAACAGAGGGAGCCAGGGACCTCATTTCAAGACTGTTGAAGCATAATCCCAGCCAGAGGCCAATGCTCAGAGAAGTACTT
GAACACCCCTGGATCACAGCAAATTCATCAAAACCATCAAATTGCCAAAACAAAGAATCAGCTAGCAAACAGTCTTAGGAATCGTGCAGGGGGAGAAATC
CTTGAGCCAGGGCTGCCATATAACCTGACAGGAACATGCTACTGAAGTTTATTTTACCATTGACTGCTGCCCTCAATCTAGAACGCTACACAAGAAATAT
TTGTTTTACTCAGCAGGTGTGCCTTAACCTCCCTATTCAGAAAGCTCCACATCAATAAACATGACACTCTGAAGTGAAAGTAGCCACGAGAATTGTGCTA
CTTATACTGGTTCATAATCTGGAGGCAAGGTTCGACTGCAGCCGCCCCGTCAGCCTGTGCTAGGCATGGTGTCTTCACAGGAGGCAAATCCAGAGCCTGG
CTGTGGGGAAAGTGACCACTCTGCCCTGACCCCGATCAGTTAAGGAGCTGTGCAATAACCTTCCTAGTACCTGAGTGAGTGTGTAACTTATTGGGTTGGC
GAAGCCTGGTAAAGCTGTTGGAATGAGTATGTGATTCTTTTTAAGTATGAAAATAAAGATATATGTACAGACTTGTATTTTTTCTCTGGTGGCATTCCTT
TAGGAATGCTGTGTGTCTGTCCGGCACCCCGGTAGGCCTGATTGGGTTTCTAGTCCTCCTTAACCACTTATCTCCCATATGAGAGTGTGAAAAATAGGAA
CACGTGCTCTACCTCCATTTAGGGATTTGCTTGGGATACAGAAGAGGCCATGTGTCTCAGAGCTGTTAAGGGCTTATTTTTTTAAAACATTGGAGTCATA
GCATGTGTGTAAACTTTAAATATGCAAATAAATAAGTATCTATGTCT
- refseq_AURKA.F3 (forward primer)
- refseq_AURKA.R3 (reverse primer)
- Alternative donor
Frame 0
TRQPRSSAGQSAF*LEGLTPI*KEEPLNSNG*ALGRLGSLGRRWEPTGG*TVGDISVADEDGGDKGR
LVGVAERQVPCRFLRP*VSLALPCARPAPLHPL
LGTEAPCRILLVTYYS*RVSLHCPGQSAGIFDK
KLQ*RPGAVAHARNPSIFGGRGWSAMV*SQLTAT
SASWV*VILLPQPPE*LGLQASWTDLKKTAFQD
LLRLQLQLEVQNVFS*LSNFLVRIHYL*IVARL
SGSCVLQILPSAFLCKHKSLSPVTSRFRIRSRSN
CRQPVYLILSPGH*ITPKRASSPCHRHLKIILR
RNWHQNRKMKNQKRGSGLWKTLKLVALWVKESL
VMFIWQEKSKASLFWLLKCYLKLSWRKPEWSISS
EEK*KYSPTFGILIFLDCMVISMMLPEST*FWN
MHHLEQFIENFRNFQSLMSRELLLI*QNWQMPC
LTVIRRELFIETLSQRTYFLDQLESLKLQILGGQ
YMLHLPGGPLSVAPWTTCPLK*LKVGCMMRRWI
SGALEFFAMNF*LGSLLLRQTHTKRPTKEYHGL
NSHSLTL*QREPGTSFQDC*SIIPARGQCSEKYL
NTPGSQQIHQNHQIAKTKNQLANSLRNRAGGEI
LEPGLPYNLTGTCY*SLFYH*LLPSI*NATQEI
FVLLSRCALTSLFRKLHINKHDTLK*K*PRELCY
LYWFIIWRQGSTAAAPSACARHGVFTGGKSRAW
LWGK*PLCPDPDQLRSCAITFLVPE*VCNLLGW
RSLVKLLE*VCDSF*V*K*RYMYRLVFFLWWHSF
RNAVCLSGTPVGLIGFLVLLNHLSPI*ECEK*E
HVLYLHLGICLGYRRGHVSQSC*GLIFLKHWSH
SMCVNFKYANK*VSMS
Frame 1
QGSLARAQANRLSS*RV*LLFKKKNL*ILTAELLEDLGPWVAGGSRRVGRPWGISQWRTRTAGTRG
GWSEWRSVKSPVGSSVPECPWRCLVPAQRLCIRS
WAPRRPVGYCLLLITARGSHSIAQARVRGYLIR
NFSEGRARWLMPVIPAFSEAEAGVQWCDLSSLQ
PLLPGFK*FSCLSLPSSWDYRHHGPI*RKLHFRT
C*GYSSSWRSKTCSRDSAISLSESITCK*WPGS
AGLVSFKFFPAHSFASTKACLQSQAGSESEAEA
IAGNQCTSSCLQATE*HPKEQAAPAIGT*K*S*G
GTGIKTEK*RIKKEAVGFGRL*NWSPSG*RKVW
*CLFGKRKAKQVYSGS*SVI*SSAGESRSGASA
QKRSRNTVPPSAS*YS*TVWLFP*CYQSLPNSGI
CTTWNSL*RTSETFKV**AENCYLYNRIGKCPV
LLSFEESYS*RH*ARELTSWISWRA*NCRFWVV
STCSIFQEDHSLWHPGLPAP*ND*RSDA**EGGS
LEPWSSLL*IFSWEASF*GKHIPRDLQKNITG*
IHIP*LCNRGSQGPHFKTVEA*SQPEANAQRST
*TPLDHSKFIKTIKLPKQRIS*QTVLGIVQGEKS
LSQGCHIT*QEHATEVYFTIDCCPQSRTLHKKY
LFYSAGVP*PPYSESSTSINMTL*SESSHENCA
TYTGS*SGGKVRLQPPRQPVLGMVSSQEANPEPG
CGESDHSALTPIS*GAVQ*PS*YLSECVTYWVG
EAW*SCWNEYVILFKYENKDICTDLYFFSGGIP
LGMLCVCPAPR*A*LGF*SSLTTYLPYESVKNRN
TCSTSI*GFAWDTEEAMCLRAVKGLFF*NIGVI
ACV*TLNMQINKYLC
Frame 2
KAASLERRPIGFLARGFNSYLKRRTFEF*RLSSWKTWVLGSQVGADGWVDRGGYLSGGRGRRGQGA
AGRSGGASSPLSVPPSLSVLGAALCPPSAFASA
PGHRGAL*DTACYLLQLEGLTPLPRPECGDI**E
TSVKAGRGGSCP*SQHFRRPRLECNGVISAHCN
LCFLGLSDSPASASRVAGITGIMDRSKENCISG
PVKATAPVGGPKRVLVTQQFPCQNPLPVNSGQAQ
RVLCPSNSSQRIPLQAQKLVSSHKPVQNQKQKQ
LQATSVPHPVSRPLNNTQKSKQPLPSAPENNPE
EELASKQKNEESKKRQWALEDFEIGRPLGKGKFG
NVYLAREKQSKFILALKVLFKAQLEKAGVEHQL
RREVEIQSHLRHPNILRLYGYFHDATRVYLILE
YAPLGTVYRELQKLSKFDEQRTATYITELANALS
YCHSKRVIHRDIKPENLLLGSAGELKIADFGWS
VHAPSSRRTTLCGTLDYLPPEMIEGRMHDEKVD
LWSLGVLCYEFLVGKPPFEANTYQETYKRISRVE
FTFPDFVTEGARDLISRLLKHNPSQRPMLREVL
EHPWITANSSKPSNCQNKESASKQS*ESCRGRN
P*ARAAI*PDRNMLLKFILPLTAALNLERYTRNI
CFTQQVCLNLPIQKAPHQ*T*HSEVKVATRIVL
LILVHNLEARFDCSRPVSLC*AWCLHRRQIQSL
AVGKVTTLP*PRSVKELCNNLPST*VSV*LIGLA
KPGKAVGMSM*FFLSMKIKIYVQTCIFSLVAFL
*ECCVSVRHPGRPDWVSSPP*PLISHMRV*KIG
TRALPPFRDLLGIQKRPCVSELLRAYFFKTLES*
HVCKL*ICK*ISIYV
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