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Sequence NM_198434
ACAAGGCAGCCTCGCTCGAGCGCAGGCCAATCGGCTTTCTAGCTAGAGGGTTTAACTCCTATTTAAAAAGAAGAACCTTTGAATTCTAACGGCTGAGCTCTTGGAAGACTTGGGTCCTTGGGTCGCAGGTGGGAGCCGACGGGGTCTCACTCCATTGCCCAGGCCAGAGTGCGGGGATATTTGATAAGAAACTTCAGTGA
AGGCCGGGCGCGGTGGCTCATGCCCGTAATCCCAGCATTTTCGGAGGCCGAGGCATCATGGACCGATCTAAAGAAAACTGCATTTCAGGACCTGTTAAGG
CTACAGCTCCAGTTGGAGGTCCAAAACGTGTTCTCGTGACTCAGCAATTTCCTTGTCAGAATCCATTACCTGTAAATAGTGGCCAGGCTCAGCGGGTCTT
GTGTCCTTCAAATTCTTCCCAGCGCATTCCTTTGCAAGCACAAAAGCTTGTCTCCAGTCACAAGCCGGTTCAGAATCAGAAGCAGAAGCAATTGCAGGCA
ACCAGTGTACCTCATCCTGTCTCCAGGCCACTGAATAACACCCAAAAGAGCAAGCAGCCCCTGCCATCGGCACCTGAAAATAATCCTGAGGAGGAACTGG
CATCAAAACAGAAAAATGAAGAATCAAAAAAGAGGCAGTGGGCTTTGGAAGACTTTGAAATTGGTCGCCCTCTGGGTAAAGGAAAGTTTGGTAATGTTTA
TTTGGCAAGAGAAAAGCAAAGCAAGTTTATTCTGGCTCTTAAAGTGTTATTTAAAGCTCAGCTGGAGAAAGCCGGAGTGGAGCATCAGCTCAGAAGAGAA
GTAGAAATACAGTCCCACCTTCGGCATCCTAATATTCTTAGACTGTATGGTTATTTCCATGATGCTACCAGAGTCTACCTAATTCTGGAATATGCACCAC
TTGGAACAGTTTATAGAGAACTTCAGAAACTTTCAAAGTTTGATGAGCAGAGAACTGCTACTTATATAACAGAATTGGCAAATGCCCTGTCTTACTGTCA
TTCGAAGAGAGTTATTCATAGAGACATTAAGCCAGAGAACTTACTTCTTGGATCAGCTGGAGAGCTTAAAATTGCAGATTTTGGGTGGTCAGTACATGCT
CCATCTTCCAGGAGGACCACTCTCTGTGGCACCCTGGACTACCTGCCCCCTGAAATGATTGAAGGTCGGATGCATGATGAGAAGGTGGATCTCTGGAGCC
TTGGAGTTCTTTGCTATGAATTTTTAGTTGGGAAGCCTCCTTTTGAGGCAAACACATACCAAGAGACCTACAAAAGAATATCACGGGTTGAATTCACATT
CCCTGACTTTGTAACAGAGGGAGCCAGGGACCTCATTTCAAGACTGTTGAAGCATAATCCCAGCCAGAGGCCAATGCTCAGAGAAGTACTTGAACACCCC
TGGATCACAGCAAATTCATCAAAACCATCAAATTGCCAAAACAAAGAATCAGCTAGCAAACAGTCTTAGGAATCGTGCAGGGGGAGAAATCCTTGAGCCA
GGGCTGCCATATAACCTGACAGGAACATGCTACTGAAGTTTATTTTACCATTGACTGCTGCCCTCAATCTAGAACGCTACACAAGAAATATTTGTTTTAC
TCAGCAGGTGTGCCTTAACCTCCCTATTCAGAAAGCTCCACATCAATAAACATGACACTCTGAAGTGAAAGTAGCCACGAGAATTGTGCTACTTATACTG
GTTCATAATCTGGAGGCAAGGTTCGACTGCAGCCGCCCCGTCAGCCTGTGCTAGGCATGGTGTCTTCACAGGAGGCAAATCCAGAGCCTGGCTGTGGGGA
AAGTGACCACTCTGCCCTGACCCCGATCAGTTAAGGAGCTGTGCAATAACCTTCCTAGTACCTGAGTGAGTGTGTAACTTATTGGGTTGGCGAAGCCTGG
TAAAGCTGTTGGAATGAGTATGTGATTCTTTTTAAGTATGAAAATAAAGATATATGTACAGACTTGTATTTTTTCTCTGGTGGCATTCCTTTAGGAATGC
TGTGTGTCTGTCCGGCACCCCGGTAGGCCTGATTGGGTTTCTAGTCCTCCTTAACCACTTATCTCCCATATGAGAGTGTGAAAAATAGGAACACGTGCTC
TACCTCCATTTAGGGATTTGCTTGGGATACAGAAGAGGCCATGTGTCTCAGAGCTGTTAAGGGCTTATTTTTTTAAAACATTGGAGTCATAGCATGTGTG
TAAACTTTAAATATGCAAATAAATAAGTATCTATGTCT
- refseq_AURKA.F3 (forward primer)
- refseq_AURKA.R3 (reverse primer)
- Alternative donor
Frame 0
TRQPRSSAGQSAF*LEGLTPI*KEEPLNSNG*ALGRLGSLGRRWEPTGSHSIAQARVRGYLIRNFSE
GRARWLMPVIPAFSEAEASWTDLKKTAFQDLLR
LQLQLEVQNVFS*LSNFLVRIHYL*IVARLSGS
CVLQILPSAFLCKHKSLSPVTSRFRIRSRSNCRQ
PVYLILSPGH*ITPKRASSPCHRHLKIILRRNW
HQNRKMKNQKRGSGLWKTLKLVALWVKESLVMF
IWQEKSKASLFWLLKCYLKLSWRKPEWSISSEEK
*KYSPTFGILIFLDCMVISMMLPEST*FWNMHH
LEQFIENFRNFQSLMSRELLLI*QNWQMPCLTV
IRRELFIETLSQRTYFLDQLESLKLQILGGQYML
HLPGGPLSVAPWTTCPLK*LKVGCMMRRWISGA
LEFFAMNF*LGSLLLRQTHTKRPTKEYHGLNSH
SLTL*QREPGTSFQDC*SIIPARGQCSEKYLNTP
GSQQIHQNHQIAKTKNQLANSLRNRAGGEILEP
GLPYNLTGTCY*SLFYH*LLPSI*NATQEIFVL
LSRCALTSLFRKLHINKHDTLK*K*PRELCYLYW
FIIWRQGSTAAAPSACARHGVFTGGKSRAWLWG
K*PLCPDPDQLRSCAITFLVPE*VCNLLGWRSL
VKLLE*VCDSF*V*K*RYMYRLVFFLWWHSFRNA
VCLSGTPVGLIGFLVLLNHLSPI*ECEK*EHVL
YLHLGICLGYRRGHVSQSC*GLIFLKHWSHSMC
VNFKYANK*VSMS
Frame 1
QGSLARAQANRLSS*RV*LLFKKKNL*ILTAELLEDLGPWVAGGSRRGLTPLPRPECGDI**ETSV
KAGRGGSCP*SQHFRRPRHHGPI*RKLHFRTC*G
YSSSWRSKTCSRDSAISLSESITCK*WPGSAGL
VSFKFFPAHSFASTKACLQSQAGSESEAEAIAG
NQCTSSCLQATE*HPKEQAAPAIGT*K*S*GGTG
IKTEK*RIKKEAVGFGRL*NWSPSG*RKVW*CL
FGKRKAKQVYSGS*SVI*SSAGESRSGASAQKR
SRNTVPPSAS*YS*TVWLFP*CYQSLPNSGICTT
WNSL*RTSETFKV**AENCYLYNRIGKCPVLLS
FEESYS*RH*ARELTSWISWRA*NCRFWVVSTC
SIFQEDHSLWHPGLPAP*ND*RSDA**EGGSLEP
WSSLL*IFSWEASF*GKHIPRDLQKNITG*IHI
P*LCNRGSQGPHFKTVEA*SQPEANAQRST*TP
LDHSKFIKTIKLPKQRIS*QTVLGIVQGEKSLSQ
GCHIT*QEHATEVYFTIDCCPQSRTLHKKYLFY
SAGVP*PPYSESSTSINMTL*SESSHENCATYT
GS*SGGKVRLQPPRQPVLGMVSSQEANPEPGCGE
SDHSALTPIS*GAVQ*PS*YLSECVTYWVGEAW
*SCWNEYVILFKYENKDICTDLYFFSGGIPLGM
LCVCPAPR*A*LGF*SSLTTYLPYESVKNRNTCS
TSI*GFAWDTEEAMCLRAVKGLFF*NIGVIACV
*TLNMQINKYLC
Frame 2
KAASLERRPIGFLARGFNSYLKRRTFEF*RLSSWKTWVLGSQVGADGVSLHCPGQSAGIFDKKLQ*
RPGAVAHARNPSIFGGRGIMDRSKENCISGPVK
ATAPVGGPKRVLVTQQFPCQNPLPVNSGQAQRVL
CPSNSSQRIPLQAQKLVSSHKPVQNQKQKQLQA
TSVPHPVSRPLNNTQKSKQPLPSAPENNPEEEL
ASKQKNEESKKRQWALEDFEIGRPLGKGKFGNVY
LAREKQSKFILALKVLFKAQLEKAGVEHQLRRE
VEIQSHLRHPNILRLYGYFHDATRVYLILEYAP
LGTVYRELQKLSKFDEQRTATYITELANALSYCH
SKRVIHRDIKPENLLLGSAGELKIADFGWSVHA
PSSRRTTLCGTLDYLPPEMIEGRMHDEKVDLWS
LGVLCYEFLVGKPPFEANTYQETYKRISRVEFTF
PDFVTEGARDLISRLLKHNPSQRPMLREVLEHP
WITANSSKPSNCQNKESASKQS*ESCRGRNP*A
RAAI*PDRNMLLKFILPLTAALNLERYTRNICFT
QQVCLNLPIQKAPHQ*T*HSEVKVATRIVLLIL
VHNLEARFDCSRPVSLC*AWCLHRRQIQSLAVG
KVTTLP*PRSVKELCNNLPST*VSV*LIGLAKPG
KAVGMSM*FFLSMKIKIYVQTCIFSLVAFL*EC
CVSVRHPGRPDWVSSPP*PLISHMRV*KIGTRA
LPPFRDLLGIQKRPCVSELLRAYFFKTLES*HVC
KL*ICK*ISIYV
Sequence NM_198433
ACAAGGCAGCCTCGCTCGAGCGCAGGCCAATCGGCTTTCTAGCTAGAGGGTTTAACTCCTATTTAAAAAGAAGAACCTTTGAATTCTAACGGCTGAGCTCTTGGAAGACTTGGGTCCTTGGGTCGCAGGTGGGAGCCGACGGGTGGGTAGACCGTGGGGGATATCTCAGTGGCGGACGAGGACGGCGGGGACAAGGGGCG
GCTGGTCGGAGTGGCGGAGCGTCAAGTCCCCTGTCGGTTCCTCCGTCCCTGAGTGTCCTTGGCGCTGCCTTGTGCCCGCCCAGCGCCTTTGCATCCGCTC
CTGGGCACCGAGGCGCCCTGTAGGATACTGCTTGTTACTTATTACAGCTAGAGGGTCTCACTCCATTGCCCAGGCCAGAGTGCGGGGATATTTGATAAGA
AACTTCAGTGAAGGCCGGGCGCGGTGGCTCATGCCCGTAATCCCAGCATTTTCGGAGGCCGAGGCTGGAGTGCAATGGTGTGATCTCAGCTCACTGCAAC
CTCTGCTTCCTGGGTTTAAGTGATTCTCCTGCCTCAGCCTCCCGAGTAGCTGGGATTACAGGCATCATGGACCGATCTAAAGAAAACTGCATTTCAGGAC
CTGTTAAGGCTACAGCTCCAGTTGGAGGTCCAAAACGTGTTCTCGTGACTCAGCAATTTCCTTGTCAGAATCCATTACCTGTAAATAGTGGCCAGGCTCA
GCGGGTCTTGTGTCCTTCAAATTCTTCCCAGCGCATTCCTTTGCAAGCACAAAAGCTTGTCTCCAGTCACAAGCCGGTTCAGAATCAGAAGCAGAAGCAA
TTGCAGGCAACCAGTGTACCTCATCCTGTCTCCAGGCCACTGAATAACACCCAAAAGAGCAAGCAGCCCCTGCCATCGGCACCTGAAAATAATCCTGAGG
AGGAACTGGCATCAAAACAGAAAAATGAAGAATCAAAAAAGAGGCAGTGGGCTTTGGAAGACTTTGAAATTGGTCGCCCTCTGGGTAAAGGAAAGTTTGG
TAATGTTTATTTGGCAAGAGAAAAGCAAAGCAAGTTTATTCTGGCTCTTAAAGTGTTATTTAAAGCTCAGCTGGAGAAAGCCGGAGTGGAGCATCAGCTC
AGAAGAGAAGTAGAAATACAGTCCCACCTTCGGCATCCTAATATTCTTAGACTGTATGGTTATTTCCATGATGCTACCAGAGTCTACCTAATTCTGGAAT
ATGCACCACTTGGAACAGTTTATAGAGAACTTCAGAAACTTTCAAAGTTTGATGAGCAGAGAACTGCTACTTATATAACAGAATTGGCAAATGCCCTGTC
TTACTGTCATTCGAAGAGAGTTATTCATAGAGACATTAAGCCAGAGAACTTACTTCTTGGATCAGCTGGAGAGCTTAAAATTGCAGATTTTGGGTGGTCA
GTACATGCTCCATCTTCCAGGAGGACCACTCTCTGTGGCACCCTGGACTACCTGCCCCCTGAAATGATTGAAGGTCGGATGCATGATGAGAAGGTGGATC
TCTGGAGCCTTGGAGTTCTTTGCTATGAATTTTTAGTTGGGAAGCCTCCTTTTGAGGCAAACACATACCAAGAGACCTACAAAAGAATATCACGGGTTGA
ATTCACATTCCCTGACTTTGTAACAGAGGGAGCCAGGGACCTCATTTCAAGACTGTTGAAGCATAATCCCAGCCAGAGGCCAATGCTCAGAGAAGTACTT
GAACACCCCTGGATCACAGCAAATTCATCAAAACCATCAAATTGCCAAAACAAAGAATCAGCTAGCAAACAGTCTTAGGAATCGTGCAGGGGGAGAAATC
CTTGAGCCAGGGCTGCCATATAACCTGACAGGAACATGCTACTGAAGTTTATTTTACCATTGACTGCTGCCCTCAATCTAGAACGCTACACAAGAAATAT
TTGTTTTACTCAGCAGGTGTGCCTTAACCTCCCTATTCAGAAAGCTCCACATCAATAAACATGACACTCTGAAGTGAAAGTAGCCACGAGAATTGTGCTA
CTTATACTGGTTCATAATCTGGAGGCAAGGTTCGACTGCAGCCGCCCCGTCAGCCTGTGCTAGGCATGGTGTCTTCACAGGAGGCAAATCCAGAGCCTGG
CTGTGGGGAAAGTGACCACTCTGCCCTGACCCCGATCAGTTAAGGAGCTGTGCAATAACCTTCCTAGTACCTGAGTGAGTGTGTAACTTATTGGGTTGGC
GAAGCCTGGTAAAGCTGTTGGAATGAGTATGTGATTCTTTTTAAGTATGAAAATAAAGATATATGTACAGACTTGTATTTTTTCTCTGGTGGCATTCCTT
TAGGAATGCTGTGTGTCTGTCCGGCACCCCGGTAGGCCTGATTGGGTTTCTAGTCCTCCTTAACCACTTATCTCCCATATGAGAGTGTGAAAAATAGGAA
CACGTGCTCTACCTCCATTTAGGGATTTGCTTGGGATACAGAAGAGGCCATGTGTCTCAGAGCTGTTAAGGGCTTATTTTTTTAAAACATTGGAGTCATA
GCATGTGTGTAAACTTTAAATATGCAAATAAATAAGTATCTATGTCT
- refseq_AURKA.F3 (forward primer)
- refseq_AURKA.R3 (reverse primer)
- Alternative donor
Frame 0
TRQPRSSAGQSAF*LEGLTPI*KEEPLNSNG*ALGRLGSLGRRWEPTGG*TVGDISVADEDGGDKGR
LVGVAERQVPCRFLRP*VSLALPCARPAPLHPL
LGTEAPCRILLVTYYS*RVSLHCPGQSAGIFDK
KLQ*RPGAVAHARNPSIFGGRGWSAMV*SQLTAT
SASWV*VILLPQPPE*LGLQASWTDLKKTAFQD
LLRLQLQLEVQNVFS*LSNFLVRIHYL*IVARL
SGSCVLQILPSAFLCKHKSLSPVTSRFRIRSRSN
CRQPVYLILSPGH*ITPKRASSPCHRHLKIILR
RNWHQNRKMKNQKRGSGLWKTLKLVALWVKESL
VMFIWQEKSKASLFWLLKCYLKLSWRKPEWSISS
EEK*KYSPTFGILIFLDCMVISMMLPEST*FWN
MHHLEQFIENFRNFQSLMSRELLLI*QNWQMPC
LTVIRRELFIETLSQRTYFLDQLESLKLQILGGQ
YMLHLPGGPLSVAPWTTCPLK*LKVGCMMRRWI
SGALEFFAMNF*LGSLLLRQTHTKRPTKEYHGL
NSHSLTL*QREPGTSFQDC*SIIPARGQCSEKYL
NTPGSQQIHQNHQIAKTKNQLANSLRNRAGGEI
LEPGLPYNLTGTCY*SLFYH*LLPSI*NATQEI
FVLLSRCALTSLFRKLHINKHDTLK*K*PRELCY
LYWFIIWRQGSTAAAPSACARHGVFTGGKSRAW
LWGK*PLCPDPDQLRSCAITFLVPE*VCNLLGW
RSLVKLLE*VCDSF*V*K*RYMYRLVFFLWWHSF
RNAVCLSGTPVGLIGFLVLLNHLSPI*ECEK*E
HVLYLHLGICLGYRRGHVSQSC*GLIFLKHWSH
SMCVNFKYANK*VSMS
Frame 1
QGSLARAQANRLSS*RV*LLFKKKNL*ILTAELLEDLGPWVAGGSRRVGRPWGISQWRTRTAGTRG
GWSEWRSVKSPVGSSVPECPWRCLVPAQRLCIRS
WAPRRPVGYCLLLITARGSHSIAQARVRGYLIR
NFSEGRARWLMPVIPAFSEAEAGVQWCDLSSLQ
PLLPGFK*FSCLSLPSSWDYRHHGPI*RKLHFRT
C*GYSSSWRSKTCSRDSAISLSESITCK*WPGS
AGLVSFKFFPAHSFASTKACLQSQAGSESEAEA
IAGNQCTSSCLQATE*HPKEQAAPAIGT*K*S*G
GTGIKTEK*RIKKEAVGFGRL*NWSPSG*RKVW
*CLFGKRKAKQVYSGS*SVI*SSAGESRSGASA
QKRSRNTVPPSAS*YS*TVWLFP*CYQSLPNSGI
CTTWNSL*RTSETFKV**AENCYLYNRIGKCPV
LLSFEESYS*RH*ARELTSWISWRA*NCRFWVV
STCSIFQEDHSLWHPGLPAP*ND*RSDA**EGGS
LEPWSSLL*IFSWEASF*GKHIPRDLQKNITG*
IHIP*LCNRGSQGPHFKTVEA*SQPEANAQRST
*TPLDHSKFIKTIKLPKQRIS*QTVLGIVQGEKS
LSQGCHIT*QEHATEVYFTIDCCPQSRTLHKKY
LFYSAGVP*PPYSESSTSINMTL*SESSHENCA
TYTGS*SGGKVRLQPPRQPVLGMVSSQEANPEPG
CGESDHSALTPIS*GAVQ*PS*YLSECVTYWVG
EAW*SCWNEYVILFKYENKDICTDLYFFSGGIP
LGMLCVCPAPR*A*LGF*SSLTTYLPYESVKNRN
TCSTSI*GFAWDTEEAMCLRAVKGLFF*NIGVI
ACV*TLNMQINKYLC
Frame 2
KAASLERRPIGFLARGFNSYLKRRTFEF*RLSSWKTWVLGSQVGADGWVDRGGYLSGGRGRRGQGA
AGRSGGASSPLSVPPSLSVLGAALCPPSAFASA
PGHRGAL*DTACYLLQLEGLTPLPRPECGDI**E
TSVKAGRGGSCP*SQHFRRPRLECNGVISAHCN
LCFLGLSDSPASASRVAGITGIMDRSKENCISG
PVKATAPVGGPKRVLVTQQFPCQNPLPVNSGQAQ
RVLCPSNSSQRIPLQAQKLVSSHKPVQNQKQKQ
LQATSVPHPVSRPLNNTQKSKQPLPSAPENNPE
EELASKQKNEESKKRQWALEDFEIGRPLGKGKFG
NVYLAREKQSKFILALKVLFKAQLEKAGVEHQL
RREVEIQSHLRHPNILRLYGYFHDATRVYLILE
YAPLGTVYRELQKLSKFDEQRTATYITELANALS
YCHSKRVIHRDIKPENLLLGSAGELKIADFGWS
VHAPSSRRTTLCGTLDYLPPEMIEGRMHDEKVD
LWSLGVLCYEFLVGKPPFEANTYQETYKRISRVE
FTFPDFVTEGARDLISRLLKHNPSQRPMLREVL
EHPWITANSSKPSNCQNKESASKQS*ESCRGRN
P*ARAAI*PDRNMLLKFILPLTAALNLERYTRNI
CFTQQVCLNLPIQKAPHQ*T*HSEVKVATRIVL
LILVHNLEARFDCSRPVSLC*AWCLHRRQIQSL
AVGKVTTLP*PRSVKELCNNLPST*VSV*LIGLA
KPGKAVGMSM*FFLSMKIKIYVQTCIFSLVAFL
*ECCVSVRHPGRPDWVSSPP*PLISHMRV*KIG
TRALPPFRDLLGIQKRPCVSELLRAYFFKTLES*
HVCKL*ICK*ISIYV
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