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Sequences

Sequence NM_001039131

GTCCACTGGGCTTGGAGTCAGTGGCAATAACCAGGGGCAATAACCAGGCGTGTCCCAGGGGGGAGCCCCGCTCTGCAGCCCTGTGCGCCGTAGAGAGCTG GACTTAGGCTGGCAGCATGGCCGAGTTCAGGGTCAGGGTGTCCACCGGAGAAGCCTTCGGGGCTGGCACATGGGACAAAGTGTCTGTCAGCATCGTGGGG ACCCGGGGAGAGAGCCCCCCACTGCCCCTGGACAATCTCGGCAAGGAGTTCACTGCGGGCGCTGAGGAGGACTTCCAGGTGACGCTCCCGGAGGACGTAG GCCGAGTGCTGCTGCTGCGCGTGCACAAGGCGCCCCCAGTGCTGCCCCTGCTGGGGCCCCTGGCCCCGGATGCCTGGTTCTGCCGCTGGTTCCAGCTGAC ACCGCCGCGGGGCGGCCACCTCCTCTTCCCCTGCTACCAGTGGCTGGAGGGGGCGGGGACCCTGGTGCTGCAGGAGGGTACAGCCAAGGTGTCCTGGGCA GACCACCACCCTGTGCTCCAGCAACAGCGCCAGGAGGAGCTTCAGGCCCGGCAGGAGATGTACCAGTGGAAGGCTTACAACCCAGGTTGGCCTCACTGCC TGGATGAAAAGACAGTGGAAGACTTGGAGCTCAATATCAAATACTCCACAGCCAAGAATGCCAACTTTTATCTACAGGCTGGCTCTGCTTTTGCAGAGAT GAAAATCAAGGGGTTGCTGGACCGCAAGGGGCTCTGGAGGAGTCTGAATGAGATGAAAAGGATCTTCAACTTCCGGAGGACCCCAGCAGCTGAGCACGCA TTTGAGCACTGGCAGGAGGACGCCTTCTTCGCCTCCCAGTTCCTGAATGGTCTCAACCCTGTCCTGATCCGCCGCTGTCACTACCTCCCAAAGAACTTCC CCGTCACTGATGCCATGGTGGCCTCAGTGTTGGGTCCTGGGACCAGCTTGCAGGCTGAGCTAGAGAAGGGCTCCCTGTTCTTGGTGGATCACGGCATCCT CTCTGGCATCCAGACCAATGTCATTAATGGGAAGCCTCAGTTCTCTGCGGCCCCAATGACCCTGCTATACCAGAGCCCAGGCTGCGGGCCGCTGCTGCCT CTCGCCATCCAGCTCAGCCAGACCCCCGGCCCAAACAGCCCCATCTTCCTGCCCACTGATGACAAGTGGGACTGGTTGCTGGCCAAGACCTGGGTGCGCA ATGCCGAGTTCTCCTTCCATGAGGCCCTCACGCACCTGCTGCACTCACATCTGCTGCCTGAGGTCTTCACCCTGGCTACCCTGCGTCAGCTGCCCCACTG CCACCCTCTCTTCAAGTCCACAGGCATCGGCATTGAAGGCTTCTCTGAGTTGATACAGAGGAACATGAAGCAGCTGAACTATTCTCTCCTGTGTCTGCCT GAGGATATCCGGACCCGAGGAGTTGAAGACATCCCAGGCTACTACTACCGTGATGATGGGATGCAGATCTGGGGTATACCCTCCTCACTGGAGACCCGGG AAGCCCTGGTGCAGTATGTCACCATGGTGATATTCACCTGCTCCGCCAAGCATGCGGCTGTCAGTGCAGGGCAGTTTGACTCCTGTGCTTGGATGCCCAA CCTGCCACCCAGCATGCAGCTGCCACCACCCACCTCCAAAGGCCTGGCAACATGCGAGGGCTTCATAGCCACCCTCCCACCTGTCAATGCCACATGTGAT GTCATCCTTGCTCTCTGGTTGCTGAGCAAGGAGCCTGGAGACCAAAGGCCCCTGGGCACCTATCCGGATGAGCACTTCACAGAGGAGGCCCCTCGGCGGA GCATCGCCACCTTCCAGAGCCGCCTGGCCCAGATCTCGAGGGGCATCCAGGAGCGGAACCAGGGCCTGGTGCTGCCCTACACCTACCTAGACCCTCCCCT CATCGAGAACAGCGTCTCCATCTAAATCCCAGGGGAACACAGGCCCAGATGACATCCCTTTGACCACATCGCTCTAGGATAACTGGCACCCAGAGAAAAG GACTCCTCAGAAAAAACAGGCCCCCATGTGCCTCTCCTGGGACAACCAGACTCTGTAACTCACCCCCACCACCATACACACACACAAAAACAGAAACAAA ATCAAAACAGAGAAAGCAGAAAATCTACCAAGAACAGAGTCTCAGGACAGAACCACTGAGTCTTTTGGAGGCTCCAAGCCTCAAAGTGCCCGCAGAGCCC ACCTTGAGGGTTTTGCTAGTTGGTTTTGTTTTGCGTTTTACAGCCGTGGGGGGAAGCACATAATCCCGCCCCAGGGCCCACTAGCATCCACTGATTGGAC CTTATGGTCACCCAACTCAAGGACAGCCACCAAGAAGTGGCTGCCAAAGAGACTGGGCGCAGTGGCTCATGCCCATAATCCCAGCACTTTGGGAGATGGA GGCGGGAAAATCATTTGAGGTCAGAAGTTCAAGGCCAGCCTGGACGACATAGCGAGACTCCACCTCTACCAAAAAATAAAAATTAAAAAACAAA

refseq_ALOX15B.F4 (forward primer)
refseq_ALOX15B.R4 (reverse primer)
Single exon skipping

Frame 0

VHWAWSQWQ*PGAITRRVPGGSPALQPCAP*RA GLRLAAWPSSGSGCPPEKPSGLAHGTKCLSASWG PGERAPHCPWTISARSSLRALRRTSR*RSRRT* AECCCCACTRRPQCCPCWGPWPRMPGSAAGSS* HRRGAATSSSPATSGWRGRGPWCCRRVQPRCPGQ TTTLCSSNSARRSFRPGRRCTSGRLTTQVGLTA WMKRQWKTWSSISNTPQPRMPTFIYRLALLLQR *KSRGCWTARGSGGV*MR*KGSSTSGGPQQLSTH LSTGRRTPSSPPSS*MVSTLS*SAAVTTSQRTS PSLMPWWPQCWVLGPACRLS*RRAPCSWWITAS SLASRPMSLMGSLSSLRPQ*PCYTRAQAAGRCCL SPSSSARPPAQTAPSSCPLMTSGTGCWPRPGCA MPSSPSMRPSRTCCTHICCLRSSPWLPCVSCPT ATLSSSPQASALKASLS*YRGT*SS*TILSCVCL RISGPEELKTSQATTTVMMGCRSGVYPPHWRPG KPWCSMSPW*YSPAPPSMRLSVQGSLTPVLGCP TCHPACSCHHPPPKAWQHARAS*PPSHLSMPHVM SSLLSGC*ARSLETKGPWAPIRMSTSQRRPLGG ASPPSRAAWPRSRGASRSGTRAWCCPTPT*TLP SSRTASPSKSQGNTGPDDIPLTTSL*DNWHPEKR TPQKKQAPMCLSWDNQTL*LTPTTIHTHKNRNK IKTEKAENLPRTESQDRTTESFGGSKPQSARRA HLEGFASWFCFAFYSRGGKHIIPPQGPLASTDWT LWSPNSRTATKKWLPKRLGAVAHAHNPSTLGDG GGKII*GQKFKASLDDIARLHLYQKIKIKKQ

Frame 1

STGLGVSGNNQGQ*PGVSQGGAPLCSPVRRREL DLGWQHGRVQGQGVHRRSLRGWHMGQSVCQHRG DPGREPPTAPGQSRQGVHCGR*GGLPGDAPGGRR PSAAAARAQGAPSAAPAGAPGPGCLVLPLVPAD TAAGRPPPLPLLPVAGGGGDPGAAGGYSQGVLG RPPPCAPATAPGGASGPAGDVPVEGLQPRLASLP G*KDSGRLGAQYQILHSQECQLLSTGWLCFCRD ENQGVAGPQGALEESE*DEKDLQLPEDPSS*AR I*ALAGGRLLRLPVPEWSQPCPDPPLSLPPKELP RH*CHGGLSVGSWDQLAG*AREGLPVLGGSRHP LWHPDQCH*WEASVLCGPNDPAIPEPRLRAAAA SRHPAQPDPRPKQPHLPAH**QVGLVAGQDLGAQ CRVLLP*GPHAPAALTSAA*GLHPGYPASAAPL PPSLQVHRHRH*RLL*VDTEEHEAAELFSPVSA *GYPDPRS*RHPRLLLP**WDADLGYTLLTGDPG SPGAVCHHGDIHLLRQACGCQCRAV*LLCLDAQ PATQHAAATTHLQRPGNMRGLHSHPPTCQCHM* CHPCSLVAEQGAWRPKAPGHLSG*ALHRGGPSAE HRHLPEPPGPDLEGHPGAEPGPGAALHLPRPSP HREQRLHLNPRGTQAQMTSL*PHRSRITGTQRK GLLRKNRPPCASPGTTRLCNSPPPPYTHTKTETK SKQRKQKIYQEQSLRTEPLSLLEAPSLKVPAEP TLRVLLVGFVLRFTAVGGST*SRPRAH*HPLIG PYGHPTQGQPPRSGCQRDWAQWLMPIIPALWEME AGKSFEVRSSRPAWTT*RDSTSTKK*KLKNK

Frame 2

PLGLESVAITRGNNQACPRGEPRSAALCAVESW T*AGSMAEFRVRVSTGEAFGAGTWDKVSVSIVG TRGESPPLPLDNLGKEFTAGAEEDFQVTLPEDV GRVLLLRVHKAPPVLPLLGPLAPDAWFCRWFQLT PPRGGHLLFPCYQWLEGAGTLVLQEGTAKVSWA DHHPVLQQQRQEELQARQEMYQWKAYNPGWPHC LDEKTVEDLELNIKYSTAKNANFYLQAGSAFAEM KIKGLLDRKGLWRSLNEMKRIFNFRRTPAAEHA FEHWQEDAFFASQFLNGLNPVLIRRCHYLPKNF PVTDAMVASVLGPGTSLQAELEKGSLFLVDHGIL SGIQTNVINGKPQFSAAPMTLLYQSPGCGPLLP LAIQLSQTPGPNSPIFLPTDDKWDWLLAKTWVR NAEFSFHEALTHLLHSHLLPEVFTLATLRQLPHC HPLFKSTGIGIEGFSELIQRNMKQLNYSLLCLP EDIRTRGVEDIPGYYYRDDGMQIWGIPSSLETR EALVQYVTMVIFTCSAKHAAVSAGQFDSCAWMPN LPPSMQLPPPTSKGLATCEGFIATLPPVNATCD VILALWLLSKEPGDQRPLGTYPDEHFTEEAPRR SIATFQSRLAQISRGIQERNQGLVLPYTYLDPPL IENSVSI*IPGEHRPR*HPFDHIALG*LAPREK DSSEKTGPHVPLLGQPDSVTHPHHHTHTQKQKQ NQNRESRKSTKNRVSGQNH*VFWRLQASKCPQSP P*GFC*LVLFCVLQPWGEAHNPAPGPTSIH*LD LMVTQLKDSHQEVAAKETGRSGSCP*SQHFGRW RRENHLRSEVQGQPGRHSETPPLPKNKN*KT

Sequence NM_001039130

GTCCACTGGGCTTGGAGTCAGTGGCAATAACCAGGGGCAATAACCAGGCGTGTCCCAGGGGGGAGCCCCGCTCTGCAGCCCTGTGCGCCGTAGAGAGCTG GACTTAGGCTGGCAGCATGGCCGAGTTCAGGGTCAGGGTGTCCACCGGAGAAGCCTTCGGGGCTGGCACATGGGACAAAGTGTCTGTCAGCATCGTGGGG ACCCGGGGAGAGAGCCCCCCACTGCCCCTGGACAATCTCGGCAAGGAGTTCACTGCGGGCGCTGAGGAGGACTTCCAGGTGACGCTCCCGGAGGACGTAG GCCGAGTGCTGCTGCTGCGCGTGCACAAGGCGCCCCCAGTGCTGCCCCTGCTGGGGCCCCTGGCCCCGGATGCCTGGTTCTGCCGCTGGTTCCAGCTGAC ACCGCCGCGGGGCGGCCACCTCCTCTTCCCCTGCTACCAGTGGCTGGAGGGGGCGGGGACCCTGGTGCTGCAGGAGGGTACAGCCAAGGTGTCCTGGGCA GACCACCACCCTGTGCTCCAGCAACAGCGCCAGGAGGAGCTTCAGGCCCGGCAGGAGATGTACCAGTGGAAGGCTTACAACCCAGGTTGGCCTCACTGCC TGGATGAAAAGACAGTGGAAGACTTGGAGCTCAATATCAAATACTCCACAGCCAAGAATGCCAACTTTTATCTACAGGCTGGCTCTGCTTTTGCAGAGAT GAAAATCAAGGGGTTGCTGGACCGCAAGGGGCTCTGGAGGAGTCTGAATGAGATGAAAAGGATCTTCAACTTCCGGAGGACCCCAGCAGCTGAGCACGCA TTTGAGCACTGGCAGGAGGACGCCTTCTTCGCCTCCCAGTTCCTGAATGGTCTCAACCCTGTCCTGATCCGCCGCTGTCACTACCTCCCAAAGAACTTCC CCGTCACTGATGCCATGGTGGCCTCAGTGTTGGGTCCTGGGACCAGCTTGCAGGCTGAGCTAGAGAAGGGCTCCCTGTTCTTGGTGGATCACGGCATCCT CTCTGGCATCCAGACCAATGTCATTAATGGGAAGCCTCAGTTCTCTGCGGCCCCAATGACCCTGCTATACCAGAGCCCAGGCTGCGGGCCGCTGCTGCCT CTCGCCATCCAGCTCAGCCAGACCCCCGGCCCAAACAGCCCCATCTTCCTGCCCACTGATGACAAGTGGGACTGGTTGCTGGCCAAGACCTGGGTGCGCA ATGCCGAGTTCTCCTTCCATGAGGCCCTCACGCACCTGCTGCACTCACATCTGCTGCCTGAGGTCTTCACCCTGGCTACCCTGCGTCAGCTGCCCCACTG CCACCCTCTCTTCAAGTCCACAGGCATCGGCATTGAAGGCTTCTCTGAGTTGATACAGAGGAACATGAAGCAGCTGAACTATTCTCTCCTGTGTCTGCCT GAGGATATCCGGACCCGAGGAGTTGAAGACATCCCAGGCTACTACTACCGTGATGATGGGATGCAGATCTGGGGTGCAGTGGAACGCTTTGTCTCTGAAA TCATCGGTATCTACTACCCAAGTGATGAGTCTGTCCAAGATGACAGAGAGCTCCAGGCCTGGGTCAGAGAGATCTTCTCCAAGGGCTTCCTAAACCAGGA GAGCTCAGGTATACCCTCCTCACTGGAGACCCGGGAAGCCCTGGTGCAGTATGTCACCATGGTGATATTCACCTGCTCCGCCAAGCATGCGGCTGTCAGT GCAGGGCAGTTTGACTCCTGTGCTTGGATGCCCAACCTGCCACCCAGCATGCAGCTGCCACCACCCACCTCCAAAGGCCTGGCAACATGCGAGGGCTTCA TAGCCACCCTCCCACCTGTCAATGCCACATGTGATGTCATCCTTGCTCTCTGGTTGCTGAGCAAGGAGCCTGGAGACCAAAGGCCCCTGGGCACCTATCC GGATGAGCACTTCACAGAGGAGGCCCCTCGGCGGAGCATCGCCACCTTCCAGAGCCGCCTGGCCCAGATCTCGAGGGGCATCCAGGAGCGGAACCAGGGC CTGGTGCTGCCCTACACCTACCTAGACCCTCCCCTCATCGAGAACAGCGTCTCCATCTAAATCCCAGGGGAACACAGGCCCAGATGACATCCCTTTGACC ACATCGCTCTAGGATAACTGGCACCCAGAGAAAAGGACTCCTCAGAAAAAACAGGCCCCCATGTGCCTCTCCTGGGACAACCAGACTCTGTAACTCACCC CCACCACCATACACACACACAAAAACAGAAACAAAATCAAAACAGAGAAAGCAGAAAATCTACCAAGAACAGAGTCTCAGGACAGAACCACTGAGTCTTT TGGAGGCTCCAAGCCTCAAAGTGCCCGCAGAGCCCACCTTGAGGGTTTTGCTAGTTGGTTTTGTTTTGCGTTTTACAGCCGTGGGGGGAAGCACATAATC CCGCCCCAGGGCCCACTAGCATCCACTGATTGGACCTTATGGTCACCCAACTCAAGGACAGCCACCAAGAAGTGGCTGCCAAAGAGACTGGGCGCAGTGG CTCATGCCCATAATCCCAGCACTTTGGGAGATGGAGGCGGGAAAATCATTTGAGGTCAGAAGTTCAAGGCCAGCCTGGACGACATAGCGAGACTCCACCT CTACCAAAAAATAAAAATTAAAAAACAAA

refseq_ALOX15B.F4 (forward primer)
refseq_ALOX15B.R4 (reverse primer)
Single exon skipping

Frame 0

VHWAWSQWQ*PGAITRRVPGGSPALQPCAP*RA GLRLAAWPSSGSGCPPEKPSGLAHGTKCLSASWG PGERAPHCPWTISARSSLRALRRTSR*RSRRT* AECCCCACTRRPQCCPCWGPWPRMPGSAAGSS* HRRGAATSSSPATSGWRGRGPWCCRRVQPRCPGQ TTTLCSSNSARRSFRPGRRCTSGRLTTQVGLTA WMKRQWKTWSSISNTPQPRMPTFIYRLALLLQR *KSRGCWTARGSGGV*MR*KGSSTSGGPQQLSTH LSTGRRTPSSPPSS*MVSTLS*SAAVTTSQRTS PSLMPWWPQCWVLGPACRLS*RRAPCSWWITAS SLASRPMSLMGSLSSLRPQ*PCYTRAQAAGRCCL SPSSSARPPAQTAPSSCPLMTSGTGCWPRPGCA MPSSPSMRPSRTCCTHICCLRSSPWLPCVSCPT ATLSSSPQASALKASLS*YRGT*SS*TILSCVCL RISGPEELKTSQATTTVMMGCRSGVQWNALSLK SSVSTTQVMSLSKMTESSRPGSERSSPRAS*TR RAQVYPPHWRPGKPWCSMSPW*YSPAPPSMRLSV QGSLTPVLGCPTCHPACSCHHPPPKAWQHARAS *PPSHLSMPHVMSSLLSGC*ARSLETKGPWAPI RMSTSQRRPLGGASPPSRAAWPRSRGASRSGTRA WCCPTPT*TLPSSRTASPSKSQGNTGPDDIPLT TSL*DNWHPEKRTPQKKQAPMCLSWDNQTL*LT PTTIHTHKNRNKIKTEKAENLPRTESQDRTTESF GGSKPQSARRAHLEGFASWFCFAFYSRGGKHII PPQGPLASTDWTLWSPNSRTATKKWLPKRLGAV AHAHNPSTLGDGGGKII*GQKFKASLDDIARLHL YQKIKIKKQ

Frame 1

STGLGVSGNNQGQ*PGVSQGGAPLCSPVRRREL DLGWQHGRVQGQGVHRRSLRGWHMGQSVCQHRG DPGREPPTAPGQSRQGVHCGR*GGLPGDAPGGRR PSAAAARAQGAPSAAPAGAPGPGCLVLPLVPAD TAAGRPPPLPLLPVAGGGGDPGAAGGYSQGVLG RPPPCAPATAPGGASGPAGDVPVEGLQPRLASLP G*KDSGRLGAQYQILHSQECQLLSTGWLCFCRD ENQGVAGPQGALEESE*DEKDLQLPEDPSS*AR I*ALAGGRLLRLPVPEWSQPCPDPPLSLPPKELP RH*CHGGLSVGSWDQLAG*AREGLPVLGGSRHP LWHPDQCH*WEASVLCGPNDPAIPEPRLRAAAA SRHPAQPDPRPKQPHLPAH**QVGLVAGQDLGAQ CRVLLP*GPHAPAALTSAA*GLHPGYPASAAPL PPSLQVHRHRH*RLL*VDTEEHEAAELFSPVSA *GYPDPRS*RHPRLLLP**WDADLGCSGTLCL*N HRYLLPK**VCPR*QRAPGLGQRDLLQGLPKPG ELRYTLLTGDPGSPGAVCHHGDIHLLRQACGCQ CRAV*LLCLDAQPATQHAAATTHLQRPGNMRGLH SHPPTCQCHM*CHPCSLVAEQGAWRPKAPGHLS G*ALHRGGPSAEHRHLPEPPGPDLEGHPGAEPG PGAALHLPRPSPHREQRLHLNPRGTQAQMTSL*P HRSRITGTQRKGLLRKNRPPCASPGTTRLCNSP PPPYTHTKTETKSKQRKQKIYQEQSLRTEPLSL LEAPSLKVPAEPTLRVLLVGFVLRFTAVGGST*S RPRAH*HPLIGPYGHPTQGQPPRSGCQRDWAQW LMPIIPALWEMEAGKSFEVRSSRPAWTT*RDST STKK*KLKNK

Frame 2

PLGLESVAITRGNNQACPRGEPRSAALCAVESW T*AGSMAEFRVRVSTGEAFGAGTWDKVSVSIVG TRGESPPLPLDNLGKEFTAGAEEDFQVTLPEDV GRVLLLRVHKAPPVLPLLGPLAPDAWFCRWFQLT PPRGGHLLFPCYQWLEGAGTLVLQEGTAKVSWA DHHPVLQQQRQEELQARQEMYQWKAYNPGWPHC LDEKTVEDLELNIKYSTAKNANFYLQAGSAFAEM KIKGLLDRKGLWRSLNEMKRIFNFRRTPAAEHA FEHWQEDAFFASQFLNGLNPVLIRRCHYLPKNF PVTDAMVASVLGPGTSLQAELEKGSLFLVDHGIL SGIQTNVINGKPQFSAAPMTLLYQSPGCGPLLP LAIQLSQTPGPNSPIFLPTDDKWDWLLAKTWVR NAEFSFHEALTHLLHSHLLPEVFTLATLRQLPHC HPLFKSTGIGIEGFSELIQRNMKQLNYSLLCLP EDIRTRGVEDIPGYYYRDDGMQIWGAVERFVSE IIGIYYPSDESVQDDRELQAWVREIFSKGFLNQE SSGIPSSLETREALVQYVTMVIFTCSAKHAAVS AGQFDSCAWMPNLPPSMQLPPPTSKGLATCEGF IATLPPVNATCDVILALWLLSKEPGDQRPLGTYP DEHFTEEAPRRSIATFQSRLAQISRGIQERNQG LVLPYTYLDPPLIENSVSI*IPGEHRPR*HPFD HIALG*LAPREKDSSEKTGPHVPLLGQPDSVTHP HHHTHTQKQKQNQNRESRKSTKNRVSGQNH*VF WRLQASKCPQSPP*GFC*LVLFCVLQPWGEAHN PAPGPTSIH*LDLMVTQLKDSHQEVAAKETGRSG SCP*SQHFGRWRRENHLRSEVQGQPGRHSETPP LPKNKN*KT

Sequence NM_001141

GTCCACTGGGCTTGGAGTCAGTGGCAATAACCAGGGGCAATAACCAGGCGTGTCCCAGGGGGGAGCCCCGCTCTGCAGCCCTGTGCGCCGTAGAGAGCTG GACTTAGGCTGGCAGCATGGCCGAGTTCAGGGTCAGGGTGTCCACCGGAGAAGCCTTCGGGGCTGGCACATGGGACAAAGTGTCTGTCAGCATCGTGGGG ACCCGGGGAGAGAGCCCCCCACTGCCCCTGGACAATCTCGGCAAGGAGTTCACTGCGGGCGCTGAGGAGGACTTCCAGGTGACGCTCCCGGAGGACGTAG GCCGAGTGCTGCTGCTGCGCGTGCACAAGGCGCCCCCAGTGCTGCCCCTGCTGGGGCCCCTGGCCCCGGATGCCTGGTTCTGCCGCTGGTTCCAGCTGAC ACCGCCGCGGGGCGGCCACCTCCTCTTCCCCTGCTACCAGTGGCTGGAGGGGGCGGGGACCCTGGTGCTGCAGGAGGGTACAGCCAAGGTGTCCTGGGCA GACCACCACCCTGTGCTCCAGCAACAGCGCCAGGAGGAGCTTCAGGCCCGGCAGGAGATGTACCAGTGGAAGGCTTACAACCCAGGTTGGCCTCACTGCC TGGATGAAAAGACAGTGGAAGACTTGGAGCTCAATATCAAATACTCCACAGCCAAGAATGCCAACTTTTATCTACAGGCTGGCTCTGCTTTTGCAGAGAT GAAAATCAAGGGGTTGCTGGACCGCAAGGGGCTCTGGAGGAGTCTGAATGAGATGAAAAGGATCTTCAACTTCCGGAGGACCCCAGCAGCTGAGCACGCA TTTGAGCACTGGCAGGAGGACGCCTTCTTCGCCTCCCAGTTCCTGAATGGTCTCAACCCTGTCCTGATCCGCCGCTGTCACTACCTCCCAAAGAACTTCC CCGTCACTGATGCCATGGTGGCCTCAGTGTTGGGTCCTGGGACCAGCTTGCAGGCTGAGCTAGAGAAGGGCTCCCTGTTCTTGGTGGATCACGGCATCCT CTCTGGCATCCAGACCAATGTCATTAATGGGAAGCCTCAGTTCTCTGCGGCCCCAATGACCCTGCTATACCAGAGCCCAGGCTGCGGGCCGCTGCTGCCT CTCGCCATCCAGCTCAGCCAGACCCCCGGCCCAAACAGCCCCATCTTCCTGCCCACTGATGACAAGTGGGACTGGTTGCTGGCCAAGACCTGGGTGCGCA ATGCCGAGTTCTCCTTCCATGAGGCCCTCACGCACCTGCTGCACTCACATCTGCTGCCTGAGGTCTTCACCCTGGCTACCCTGCGTCAGCTGCCCCACTG CCACCCTCTCTTCAAGCTGCTGATCCCGCACACCCGATACACCCTGCACATCAACACACTCGCCCGGGAGCTGCTTATCGTGCCAGGGCAGGTGGTGGAC AGGTCCACAGGCATCGGCATTGAAGGCTTCTCTGAGTTGATACAGAGGAACATGAAGCAGCTGAACTATTCTCTCCTGTGTCTGCCTGAGGATATCCGGA CCCGAGGAGTTGAAGACATCCCAGGCTACTACTACCGTGATGATGGGATGCAGATCTGGGGTGCAGTGGAACGCTTTGTCTCTGAAATCATCGGTATCTA CTACCCAAGTGATGAGTCTGTCCAAGATGACAGAGAGCTCCAGGCCTGGGTCAGAGAGATCTTCTCCAAGGGCTTCCTAAACCAGGAGAGCTCAGGTATA CCCTCCTCACTGGAGACCCGGGAAGCCCTGGTGCAGTATGTCACCATGGTGATATTCACCTGCTCCGCCAAGCATGCGGCTGTCAGTGCAGGGCAGTTTG ACTCCTGTGCTTGGATGCCCAACCTGCCACCCAGCATGCAGCTGCCACCACCCACCTCCAAAGGCCTGGCAACATGCGAGGGCTTCATAGCCACCCTCCC ACCTGTCAATGCCACATGTGATGTCATCCTTGCTCTCTGGTTGCTGAGCAAGGAGCCTGGAGACCAAAGGCCCCTGGGCACCTATCCGGATGAGCACTTC ACAGAGGAGGCCCCTCGGCGGAGCATCGCCACCTTCCAGAGCCGCCTGGCCCAGATCTCGAGGGGCATCCAGGAGCGGAACCAGGGCCTGGTGCTGCCCT ACACCTACCTAGACCCTCCCCTCATCGAGAACAGCGTCTCCATCTAAATCCCAGGGGAACACAGGCCCAGATGACATCCCTTTGACCACATCGCTCTAGG ATAACTGGCACCCAGAGAAAAGGACTCCTCAGAAAAAACAGGCCCCCATGTGCCTCTCCTGGGACAACCAGACTCTGTAACTCACCCCCACCACCATACA CACACACAAAAACAGAAACAAAATCAAAACAGAGAAAGCAGAAAATCTACCAAGAACAGAGTCTCAGGACAGAACCACTGAGTCTTTTGGAGGCTCCAAG CCTCAAAGTGCCCGCAGAGCCCACCTTGAGGGTTTTGCTAGTTGGTTTTGTTTTGCGTTTTACAGCCGTGGGGGGAAGCACATAATCCCGCCCCAGGGCC CACTAGCATCCACTGATTGGACCTTATGGTCACCCAACTCAAGGACAGCCACCAAGAAGTGGCTGCCAAAGAGACTGGGCGCAGTGGCTCATGCCCATAA TCCCAGCACTTTGGGAGATGGAGGCGGGAAAATCATTTGAGGTCAGAAGTTCAAGGCCAGCCTGGACGACATAGCGAGACTCCACCTCTACCAAAAAATA AAAATTAAAAAACAAA

refseq_ALOX15B.F4 (forward primer)
refseq_ALOX15B.R4 (reverse primer)
Single exon skipping

Frame 0

VHWAWSQWQ*PGAITRRVPGGSPALQPCAP*RA GLRLAAWPSSGSGCPPEKPSGLAHGTKCLSASWG PGERAPHCPWTISARSSLRALRRTSR*RSRRT* AECCCCACTRRPQCCPCWGPWPRMPGSAAGSS* HRRGAATSSSPATSGWRGRGPWCCRRVQPRCPGQ TTTLCSSNSARRSFRPGRRCTSGRLTTQVGLTA WMKRQWKTWSSISNTPQPRMPTFIYRLALLLQR *KSRGCWTARGSGGV*MR*KGSSTSGGPQQLSTH LSTGRRTPSSPPSS*MVSTLS*SAAVTTSQRTS PSLMPWWPQCWVLGPACRLS*RRAPCSWWITAS SLASRPMSLMGSLSSLRPQ*PCYTRAQAAGRCCL SPSSSARPPAQTAPSSCPLMTSGTGCWPRPGCA MPSSPSMRPSRTCCTHICCLRSSPWLPCVSCPT ATLSSSC*SRTPDTPCTSTHSPGSCLSCQGRWWT GPQASALKASLS*YRGT*SS*TILSCVCLRISG PEELKTSQATTTVMMGCRSGVQWNALSLKSSVS TTQVMSLSKMTESSRPGSERSSPRAS*TRRAQVY PPHWRPGKPWCSMSPW*YSPAPPSMRLSVQGSL TPVLGCPTCHPACSCHHPPPKAWQHARAS*PPS HLSMPHVMSSLLSGC*ARSLETKGPWAPIRMSTS QRRPLGGASPPSRAAWPRSRGASRSGTRAWCCP TPT*TLPSSRTASPSKSQGNTGPDDIPLTTSL* DNWHPEKRTPQKKQAPMCLSWDNQTL*LTPTTIH THKNRNKIKTEKAENLPRTESQDRTTESFGGSK PQSARRAHLEGFASWFCFAFYSRGGKHIIPPQG PLASTDWTLWSPNSRTATKKWLPKRLGAVAHAHN PSTLGDGGGKII*GQKFKASLDDIARLHLYQKI KIKKQ

Frame 1

STGLGVSGNNQGQ*PGVSQGGAPLCSPVRRREL DLGWQHGRVQGQGVHRRSLRGWHMGQSVCQHRG DPGREPPTAPGQSRQGVHCGR*GGLPGDAPGGRR PSAAAARAQGAPSAAPAGAPGPGCLVLPLVPAD TAAGRPPPLPLLPVAGGGGDPGAAGGYSQGVLG RPPPCAPATAPGGASGPAGDVPVEGLQPRLASLP G*KDSGRLGAQYQILHSQECQLLSTGWLCFCRD ENQGVAGPQGALEESE*DEKDLQLPEDPSS*AR I*ALAGGRLLRLPVPEWSQPCPDPPLSLPPKELP RH*CHGGLSVGSWDQLAG*AREGLPVLGGSRHP LWHPDQCH*WEASVLCGPNDPAIPEPRLRAAAA SRHPAQPDPRPKQPHLPAH**QVGLVAGQDLGAQ CRVLLP*GPHAPAALTSAA*GLHPGYPASAAPL PPSLQAADPAHPIHPAHQHTRPGAAYRARAGGG QVHRHRH*RLL*VDTEEHEAAELFSPVSA*GYPD PRS*RHPRLLLP**WDADLGCSGTLCL*NHRYL LPK**VCPR*QRAPGLGQRDLLQGLPKPGELRY TLLTGDPGSPGAVCHHGDIHLLRQACGCQCRAV* LLCLDAQPATQHAAATTHLQRPGNMRGLHSHPP TCQCHM*CHPCSLVAEQGAWRPKAPGHLSG*AL HRGGPSAEHRHLPEPPGPDLEGHPGAEPGPGAAL HLPRPSPHREQRLHLNPRGTQAQMTSL*PHRSR ITGTQRKGLLRKNRPPCASPGTTRLCNSPPPPY THTKTETKSKQRKQKIYQEQSLRTEPLSLLEAPS LKVPAEPTLRVLLVGFVLRFTAVGGST*SRPRA H*HPLIGPYGHPTQGQPPRSGCQRDWAQWLMPI IPALWEMEAGKSFEVRSSRPAWTT*RDSTSTKK* KLKNK

Frame 2

PLGLESVAITRGNNQACPRGEPRSAALCAVESW T*AGSMAEFRVRVSTGEAFGAGTWDKVSVSIVG TRGESPPLPLDNLGKEFTAGAEEDFQVTLPEDV GRVLLLRVHKAPPVLPLLGPLAPDAWFCRWFQLT PPRGGHLLFPCYQWLEGAGTLVLQEGTAKVSWA DHHPVLQQQRQEELQARQEMYQWKAYNPGWPHC LDEKTVEDLELNIKYSTAKNANFYLQAGSAFAEM KIKGLLDRKGLWRSLNEMKRIFNFRRTPAAEHA FEHWQEDAFFASQFLNGLNPVLIRRCHYLPKNF PVTDAMVASVLGPGTSLQAELEKGSLFLVDHGIL SGIQTNVINGKPQFSAAPMTLLYQSPGCGPLLP LAIQLSQTPGPNSPIFLPTDDKWDWLLAKTWVR NAEFSFHEALTHLLHSHLLPEVFTLATLRQLPHC HPLFKLLIPHTRYTLHINTLARELLIVPGQVVD RSTGIGIEGFSELIQRNMKQLNYSLLCLPEDIR TRGVEDIPGYYYRDDGMQIWGAVERFVSEIIGIY YPSDESVQDDRELQAWVREIFSKGFLNQESSGI PSSLETREALVQYVTMVIFTCSAKHAAVSAGQF DSCAWMPNLPPSMQLPPPTSKGLATCEGFIATLP PVNATCDVILALWLLSKEPGDQRPLGTYPDEHF TEEAPRRSIATFQSRLAQISRGIQERNQGLVLP YTYLDPPLIENSVSI*IPGEHRPR*HPFDHIALG *LAPREKDSSEKTGPHVPLLGQPDSVTHPHHHT HTQKQKQNQNRESRKSTKNRVSGQNH*VFWRLQ ASKCPQSPP*GFC*LVLFCVLQPWGEAHNPAPGP TSIH*LDLMVTQLKDSHQEVAAKETGRSGSCP* SQHFGRWRRENHLRSEVQGQPGRHSETPPLPKN KN*KT