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Sequence NM_178270
CGCCTTTGCTGCCCTCACAGACTTGGCCCCTAGCAGTGCAGAACTACAAGTCCCAGGGATCCTAGCGACCGTCCGTCCGTAGTCAAGTTGCCGGTGGAATTGGCCCAGGATGACAGCTGGAGAATGGAGTCAGTTTTATCCAAGTATGAAGATCAGATTACTATTTTCACTGACTACCTAGAAGAATATCCAGATACAGA
TGAGCTGGTATGGATCTTAGGGAAGCAGCATCTCCTTAAAACAGAAAAATCTAAGCTGTTGTCTGATATAAGTGCTCGTCTATGGTTTACATACAGAAGG
AAATTTTCACCAATTGGTGGAACGGGCCCTTCATCAGATGCTGGTTGGGGATGTATGCTACGCTGTGGACAGATGATGCTGGCTCAAGCCCTTATCTGTA
GACACTTGGGAAGGGACTGGAGCTGGGAGAAACAAAAAGAACAACCCAAAGAATACCAACGCATCCTACAGTGCTTCTTAGATAGAAAAGATTGTTGCTA
CTCTATCCATCAAATGGCACAAATGGGTGTAGGAGAAGGGAAATCAATTGGAGAATGGTTTGGACCAAATACAGTTGCACAGGTGTTAAAAAAACTTGCT
TTATTTGACGAATGGAATTCCTTGGCTGTTTATGTTTCAATGGATAACACAGTGGTCATTGAAGATATCAAAAAAATGTGCCGTGTCCTTCCCTTGAGTG
CTGACACAGCTGGTGACAGGCCTCCCGATTCTTTAACTGCTTCAAACCAGAGTGACGAGCTCATCTTCTTGGACCCTCATACAACCCAGACCTTTGTTGA
CACTGAAGAGAATGGAACGGTTAATGACCAGACTTTCCATTGCCTGCAGTCCCCACAGCGAATGAACATCCTAAACCTGGATCCTTCAGTTGCATTGGGA
TTTTTCTGCAAAGAAGAAAAAGACTTTGATAACTGGTGTAGCCTTGTTCAGAAGGAAATTCTAAAGGAGAATTTAAGGATGTTTGAATTAGTTCAGAAAC
ATCCATCACACTGGCCTCCCTTTGTACCTCCAGCCAAGCCAGAAGTGACAACCACTGGGGCAGAATTCATTGACTCTACTGAGCAACTGGAGGAGTTTGA
TCTGGAGGAAGATTTTGAGATTCTGAGTGTGTAGAATCCTGGGAACTCAACTTGAAGGTCTGTCTTCCATCTGGCACCATAAAAACATGAACTTATTGCA
TAAAACTTTTCTAGTCAGCAAGTGCCTGATATGCCAATAGCATACAAACTCAATAGCAATCATGACTGAGCCAATCACTGTTTCTCAGAAAAACAAAACA
AAACAAAACAAATGACAGTAACCCTTCCCCGGAAAGAAATAGAACAATCATGGAGCCTAGGAGCAGAGAGATGAGGAGGAGTTCATTGCTTCCCAGCTTG
TGTTATATGGCTACAGCAAGTCTTCAGCTGCTGCAATGAGGAAATGGGCATCTGGAAGACAAACAGCAACTCTCAGCTTGCTTCAAGAACCAGCAGATAA
GAGATGGTTAAGCTGTTCTTCACCCTTTCAGATGTGACCTCTTTTGGACTAAGCAGCAATCTGTTCTCTTGCTCAAATAATAAAGTGACTGAATCAGGGA
GGAAAAGGTTCTTGTTAAATTATTTGATTGTGTAGTTGAAGTAATTATAATTTATATCAAAACGTTTGTCAAAGAAACGATGTCAAATATACACTTCTTG
ATCTCCCTTCTGTTTGCGGGGATCTTACTATTTGATGGGTCACTGTCCCCATTCTTACTGATACTTTTGTCAGATATCACCCTGTCCTTAAATCATGATC
ACTTAAATCAGGGGTCAGCAAACTTTTTCTGTAAAGGGCCAGACGGGAAATATTTTGGGCTTTGCAGGCCATGCGGCCTCTGTCACATCTACTCAACTCT
GCTGTTGACATGCAAAAGCAGCAATAGACAATATGCGTGTAAATGAGTGTGGCTGTAATCCAAGAAAACTTTATTTACAAAAGCAGGTGGAGGGCTGGGT
TTGGCCTGCAGGCTGTAGCTTGCCAATCAGTGACTTAAATTGTTGATTTTTGTTTGATAAATTAAAAATAAATTGTGTTTGAAGTATA
- refseq_ATG4A.F1 (forward primer)
- refseq_ATG4A.R1 (reverse primer)
- Exon skipping and alternative donor or acceptor
Frame 0
RLCCPHRLGP*QCRTTSPRDPSDRPSVVKLPVELAQDDSWRMESVLSKYEDQITIFTDYLEEYPDTD
ELVWILGKQHLLKTEKSKLLSDISARLWFTYRR
KFSPIGGTGPSSDAGWGCMLRCGQMMLAQALIC
RHLGRDWSWEKQKEQPKEYQRILQCFLDRKDCCY
SIHQMAQMGVGEGKSIGEWFGPNTVAQVLKKLA
LFDEWNSLAVYVSMDNTVVIEDIKKMCRVLPLS
ADTAGDRPPDSLTASNQSDELIFLDPHTTQTFVD
TEENGTVNDQTFHCLQSPQRMNILNLDPSVALG
FFCKEEKDFDNWCSLVQKEILKENLRMFELVQK
HPSHWPPFVPPAKPEVTTTGAEFIDSTEQLEEFD
LEEDFEILSV*NPGNST*RSVFHLAP*KHELIA
*NFSSQQVPDMPIAYKLNSNHD*ANHCFSEKQN
KTKQMTVTLPRKEIEQSWSLGAER*GGVHCFPAC
VIWLQQVFSCCNEEMGIWKTNSNSQLASRTSR*
EMVKLFFTLSDVTSFGLSSNLFSCSNNKVTESG
RKRFLLNYLIV*LK*L*FISKRLSKKRCQIYTS*
SPFCLRGSYYLMGHCPHSY*YFCQISPCP*IMI
T*IRGQQTFSVKGQTGNILGFAGHAASVTSTQL
CC*HAKAAIDNMRVNECGCNPRKLYLQKQVEGWV
WPAGCSLPISDLNC*FLFDKLKINCV*SI
Frame 1
AFAALTDLAPSSAELQVPGILATVRP*SSCRWNWPRMTAGEWSQFYPSMKIRLLFSLTT*KNIQIQ
MSWYGS*GSSISLKQKNLSCCLI*VLVYGLHTEG
NFHQLVERALHQMLVGDVCYAVDR*CWLKPLSV
DTWEGTGAGRNKKNNPKNTNASYSAS*IEKIVA
TLSIKWHKWV*EKGNQLENGLDQIQLHRC*KNLL
YLTNGIPWLFMFQWITQWSLKISKKCAVSFP*V
LTQLVTGLPIL*LLQTRVTSSSSWTLIQPRPLL
TLKRMERLMTRLSIACSPHSE*TS*TWILQLHWD
FSAKKKKTLITGVALFRRKF*RRI*GCLN*FRN
IHHTGLPLYLQPSQK*QPLGQNSLTLLSNWRSL
IWRKILRF*VCRILGTQLEGLSSIWHHKNMNLLH
KTFLVSKCLICQ*HTNSIAIMTEPITVSQKNKT
KQNK*Q*PFPGKK*NNHGA*EQRDEEEFIASQL
VLYGYSKSSAAAMRKWASGRQTATLSLLQEPADK
RWLSCSSPFQM*PLLD*AAICSLAQIIK*LNQG
GKGSC*II*LCS*SNYNLYQNVCQRNDVKYTLL
DLPSVCGDLTI*WVTVPILTDTFVRYHPVLKS*S
LKSGVSKLFL*RARREIFWALQAMRPLSHLLNS
AVDMQKQQ*TICV*MSVAVIQENFIYKSRWRAG
FGLQAVACQSVT*IVDFCLIN*K*IVFEV
Frame 2
PLLPSQTWPLAVQNYKSQGS*RPSVRSQVAGGIGPG*QLENGVSFIQV*RSDYYFH*LPRRISRYR
*AGMDLREAASP*NRKI*AVV*YKCSSMVYIQK
EIFTNWWNGPFIRCWLGMYATLWTDDAGSSPYL*
TLGKGLELGETKRTTQRIPTHPTVLLR*KRLLL
LYPSNGTNGCRRREINWRMVWTKYSCTGVKKTC
FI*RMEFLGCLCFNG*HSGH*RYQKNVPCPSLEC
*HSW*QASRFFNCFKPE*RAHLLGPSYNPDLC*
H*REWNG**PDFPLPAVPTANEHPKPGSFSCIG
IFLQRRKRL**LV*PCSEGNSKGEFKDV*ISSET
SITLASLCTSSQARSDNHWGRIH*LY*ATGGV*
SGGRF*DSECVESWELNLKVCLPSGTIKT*TYC
IKLF*SASA*YANSIQTQ*QS*LSQSLFLRKTKQ
NKTNDSNPSPERNRTIMEPRSREMRRSSLLPSL
CYMATASLQLLQ*GNGHLEDKQQLSACFKNQQI
RDG*AVLHPFRCDLFWTKQQSVLLLK**SD*IRE
EKVLVKLFDCVVEVIIIYIKTFVKETMSNIHFL
ISLLFAGILLFDGSLSPFLLILLSDITLSLNHD
HLNQGSANFFCKGPDGKYFGLCRPCGLCHIYSTL
LLTCKSSNRQYACK*VWL*SKKTLFTKAGGGLG
LACRL*LANQ*LKLLIFV**IKNKLCLKY
Sequence NM_052936
CGCCTTTGCTGCCCTCACAGACTTGGCCCCTAGCAGTGCAGAACTACAAGTCCCAGGGATCCTAGCGACCGTCCGTCCGTAGTCAAGTTGCCGGTGGAATTGGCCCAGGATGACAGCTGGAGAATGGAGTCAGTTTTATCCAAGTATGAAGATCAGATTACTATTTTCACTGACTACCTAGAAGAATATCCAGATACAGA
TGAGCTGGTATGGATCTTAGGGAAGCAGCATCTCCTTAAAACAGAAAAATCTAAGCTGTTGTCTGATATAAGTGCTCGTCTATGGTTTACATACAGAAGG
AAATTTTCACCAATTGGTGGAACGGGCCCTTCATCAGATGCTGGTTGGGGATGTATGCTACGCTGTGGACAGATGATGCTGGCTCAAGCCCTTATCTGTA
GACACTTGGGAAGGGACTGGAGCTGGGAGAAACAAAAAGAACAACCCAAAGAATACCAACGCATCCTACAGTGCTTCTTAGATAGAAAAGATTGTTGCTA
CTCTATCCATCAAATGGCACAAATGGGTGTAGGAGAAGGGAAATCAATTGGAGAATGGTTTGGACCAAATACAGTTGCACAGGTGTTAAAAAAACTTGCT
TTATTTGACGAATGGAATTCCTTGGCTGTTTATGTTTCAATGGATAACACAGTGGTCATTGAAGATATCAAAAAAATGTGCCGTGTCCTTCCCTTGAGTG
CTGACACAGCTGGTGACAGGCCTCCCGATTCTTTAACTGCTTCAAACCAGAGTAAGGGCACCTCTGCCTACTGCTCAGCCTGGAAACCCCTGCTGCTCAT
TGTGCCCCTTCGCCTGGGCATAAACCAAATCAATCCTGTCTATGTTGATGCATTCAAAGAGTGTTTTAAGATGCCACAGTCTTTAGGGGCATTAGGAGGA
AAACCAAATAACGCGTATTATTTCATAGGATTCTTAGGTGACGAGCTCATCTTCTTGGACCCTCATACAACCCAGACCTTTGTTGACACTGAAGAGAATG
GAACGGTTAATGACCAGACTTTCCATTGCCTGCAGTCCCCACAGCGAATGAACATCCTAAACCTGGATCCTTCAGTTGCATTGGGATTTTTCTGCAAAGA
AGAAAAAGACTTTGATAACTGGTGTAGCCTTGTTCAGAAGGAAATTCTAAAGGAGAATTTAAGGATGTTTGAATTAGTTCAGAAACATCCATCACACTGG
CCTCCCTTTGTACCTCCAGCCAAGCCAGAAGTGACAACCACTGGGGCAGAATTCATTGACTCTACTGAGCAACTGGAGGAGTTTGATCTGGAGGAAGATT
TTGAGATTCTGAGTGTGTAGAATCCTGGGAACTCAACTTGAAGGTCTGTCTTCCATCTGGCACCATAAAAACATGAACTTATTGCATAAAACTTTTCTAG
TCAGCAAGTGCCTGATATGCCAATAGCATACAAACTCAATAGCAATCATGACTGAGCCAATCACTGTTTCTCAGAAAAACAAAACAAAACAAAACAAATG
ACAGTAACCCTTCCCCGGAAAGAAATAGAACAATCATGGAGCCTAGGAGCAGAGAGATGAGGAGGAGTTCATTGCTTCCCAGCTTGTGTTATATGGCTAC
AGCAAGTCTTCAGCTGCTGCAATGAGGAAATGGGCATCTGGAAGACAAACAGCAACTCTCAGCTTGCTTCAAGAACCAGCAGATAAGAGATGGTTAAGCT
GTTCTTCACCCTTTCAGATGTGACCTCTTTTGGACTAAGCAGCAATCTGTTCTCTTGCTCAAATAATAAAGTGACTGAATCAGGGAGGAAAAGGTTCTTG
TTAAATTATTTGATTGTGTAGTTGAAGTAATTATAATTTATATCAAAACGTTTGTCAAAGAAACGATGTCAAATATACACTTCTTGATCTCCCTTCTGTT
TGCGGGGATCTTACTATTTGATGGGTCACTGTCCCCATTCTTACTGATACTTTTGTCAGATATCACCCTGTCCTTAAATCATGATCACTTAAATCAGGGG
TCAGCAAACTTTTTCTGTAAAGGGCCAGACGGGAAATATTTTGGGCTTTGCAGGCCATGCGGCCTCTGTCACATCTACTCAACTCTGCTGTTGACATGCA
AAAGCAGCAATAGACAATATGCGTGTAAATGAGTGTGGCTGTAATCCAAGAAAACTTTATTTACAAAAGCAGGTGGAGGGCTGGGTTTGGCCTGCAGGCT
GTAGCTTGCCAATCAGTGACTTAAATTGTTGATTTTTGTTTGATAAATTAAAAATAAATTGTGTTTGAAGTATA
- refseq_ATG4A.F1 (forward primer)
- refseq_ATG4A.R1 (reverse primer)
- Exon skipping and alternative donor or acceptor
Frame 0
RLCCPHRLGP*QCRTTSPRDPSDRPSVVKLPVELAQDDSWRMESVLSKYEDQITIFTDYLEEYPDTD
ELVWILGKQHLLKTEKSKLLSDISARLWFTYRR
KFSPIGGTGPSSDAGWGCMLRCGQMMLAQALIC
RHLGRDWSWEKQKEQPKEYQRILQCFLDRKDCCY
SIHQMAQMGVGEGKSIGEWFGPNTVAQVLKKLA
LFDEWNSLAVYVSMDNTVVIEDIKKMCRVLPLS
ADTAGDRPPDSLTASNQSKGTSAYCSAWKPLLLI
VPLRLGINQINPVYVDAFKECFKMPQSLGALGG
KPNNAYYFIGFLGDELIFLDPHTTQTFVDTEEN
GTVNDQTFHCLQSPQRMNILNLDPSVALGFFCKE
EKDFDNWCSLVQKEILKENLRMFELVQKHPSHW
PPFVPPAKPEVTTTGAEFIDSTEQLEEFDLEED
FEILSV*NPGNST*RSVFHLAP*KHELIA*NFSS
QQVPDMPIAYKLNSNHD*ANHCFSEKQNKTKQM
TVTLPRKEIEQSWSLGAER*GGVHCFPACVIWL
QQVFSCCNEEMGIWKTNSNSQLASRTSR*EMVKL
FFTLSDVTSFGLSSNLFSCSNNKVTESGRKRFL
LNYLIV*LK*L*FISKRLSKKRCQIYTS*SPFC
LRGSYYLMGHCPHSY*YFCQISPCP*IMIT*IRG
QQTFSVKGQTGNILGFAGHAASVTSTQLCC*HA
KAAIDNMRVNECGCNPRKLYLQKQVEGWVWPAG
CSLPISDLNC*FLFDKLKINCV*SI
Frame 1
AFAALTDLAPSSAELQVPGILATVRP*SSCRWNWPRMTAGEWSQFYPSMKIRLLFSLTT*KNIQIQ
MSWYGS*GSSISLKQKNLSCCLI*VLVYGLHTEG
NFHQLVERALHQMLVGDVCYAVDR*CWLKPLSV
DTWEGTGAGRNKKNNPKNTNASYSAS*IEKIVA
TLSIKWHKWV*EKGNQLENGLDQIQLHRC*KNLL
YLTNGIPWLFMFQWITQWSLKISKKCAVSFP*V
LTQLVTGLPIL*LLQTRVRAPLPTAQPGNPCCS
LCPFAWA*TKSILSMLMHSKSVLRCHSL*GH*EE
NQITRIIS*DS*VTSSSSWTLIQPRPLLTLKRM
ERLMTRLSIACSPHSE*TS*TWILQLHWDFSAK
KKKTLITGVALFRRKF*RRI*GCLN*FRNIHHTG
LPLYLQPSQK*QPLGQNSLTLLSNWRSLIWRKI
LRF*VCRILGTQLEGLSSIWHHKNMNLLHKTFL
VSKCLICQ*HTNSIAIMTEPITVSQKNKTKQNK*
Q*PFPGKK*NNHGA*EQRDEEEFIASQLVLYGY
SKSSAAAMRKWASGRQTATLSLLQEPADKRWLS
CSSPFQM*PLLD*AAICSLAQIIK*LNQGGKGSC
*II*LCS*SNYNLYQNVCQRNDVKYTLLDLPSV
CGDLTI*WVTVPILTDTFVRYHPVLKS*SLKSG
VSKLFL*RARREIFWALQAMRPLSHLLNSAVDMQ
KQQ*TICV*MSVAVIQENFIYKSRWRAGFGLQA
VACQSVT*IVDFCLIN*K*IVFEV
Frame 2
PLLPSQTWPLAVQNYKSQGS*RPSVRSQVAGGIGPG*QLENGVSFIQV*RSDYYFH*LPRRISRYR
*AGMDLREAASP*NRKI*AVV*YKCSSMVYIQK
EIFTNWWNGPFIRCWLGMYATLWTDDAGSSPYL*
TLGKGLELGETKRTTQRIPTHPTVLLR*KRLLL
LYPSNGTNGCRRREINWRMVWTKYSCTGVKKTC
FI*RMEFLGCLCFNG*HSGH*RYQKNVPCPSLEC
*HSW*QASRFFNCFKPE*GHLCLLLSLETPAAH
CAPSPGHKPNQSCLC*CIQRVF*DATVFRGIRR
KTK*RVLFHRILR*RAHLLGPSYNPDLC*H*REW
NG**PDFPLPAVPTANEHPKPGSFSCIGIFLQR
RKRL**LV*PCSEGNSKGEFKDV*ISSETSITL
ASLCTSSQARSDNHWGRIH*LY*ATGGV*SGGRF
*DSECVESWELNLKVCLPSGTIKT*TYCIKLF*
SASA*YANSIQTQ*QS*LSQSLFLRKTKQNKTN
DSNPSPERNRTIMEPRSREMRRSSLLPSLCYMAT
ASLQLLQ*GNGHLEDKQQLSACFKNQQIRDG*A
VLHPFRCDLFWTKQQSVLLLK**SD*IREEKVL
VKLFDCVVEVIIIYIKTFVKETMSNIHFLISLLF
AGILLFDGSLSPFLLILLSDITLSLNHDHLNQG
SANFFCKGPDGKYFGLCRPCGLCHIYSTLLLTC
KSSNRQYACK*VWL*SKKTLFTKAGGGLGLACRL
*LANQ*LKLLIFV**IKNKLCLKY
Sequence NM_178271
CGCCTTTGCTGCCCTCACAGACTTGGCCCCTAGCAGTGCAGAACTACAAGTCCCAGGGATCCTAGCGACCGTCCGTCCGTAGTCAAGTTGCCGGTGGAATTGGCCCAGGATGACAGCTGGAGAATGGAGTCAGTTTTATCCAAGTATGAAGATCAGATTACTATTTTCACTGACTACCTAGAAGAATATCCAGATACAGA
TGAGCTGGTATGGATCTTAGGGAAGCAGCATCTCCTTAAAACAGATTGGGAAAGAAAGACCTTTTAACCTGAAACTGCTAACAGCCCAGCACCCAGATAA
AGCCAGAGCTTGGAAAGAGGCAGCTCTGAGATTTGTGTCTTTCTGAGGAGACATCCTCTTCAGAGGCCCTCACTTAGCGTGGAGGAAGGAACAGGAAGAG
GCTGATGAAAAGGCAGGAGAGGAAGTTAGGCATTGTGGAGCTCCTCCTGAGCCTGGCTTCATTATTTTGATTGGAGAATTCTGAACAGTGGAAAGCCTGG
AGCTAGCAATTAGGAGACCTTTTGTATTCAGACTCTTCTAACTCAGAAAAATCTAAGCTGTTGTCTGATATAAGTGCTCGTCTATGGTTTACATACAGAA
GGAAATTTTCACCAATTGGTGGAACGGGCCCTTCATCAGATGCTGGTTGGGGATGTATGCTACGCTGTGGACAGATGATGCTGGCTCAAGCCCTTATCTG
TAGACACTTGGGAAGGGACTGGAGCTGGGAGAAACAAAAAGAACAACCCAAAGAATACCAACGCATCCTACAGTGCTTCTTAGATAGAAAAGATTGTTGC
TACTCTATCCATCAAATGGCACAAATGGGTGTAGGAGAAGGGAAATCAATTGGAGAATGGTTTGGACCAAATACAGTTGCACAGGTGTTAAAAAAACTTG
CTTTATTTGACGAATGGAATTCCTTGGCTGTTTATGTTTCAATGGATAACACAGTGGTCATTGAAGATATCAAAAAAATGTGCCGTGTCCTTCCCTTGAG
TGCTGACACAGCTGGTGACAGGCCTCCCGATTCTTTAACTGCTTCAAACCAGAGTAAGGGCACCTCTGCCTACTGCTCAGCCTGGAAACCCCTGCTGCTC
ATTGTGCCCCTTCGCCTGGGCATAAACCAAATCAATCCTGTCTATGTTGATGCATTCAAAGAGTGTTTTAAGATGCCACAGTCTTTAGGGGCATTAGGAG
GAAAACCAAATAACGCGTATTATTTCATAGGATTCTTAGGTGACGAGCTCATCTTCTTGGACCCTCATACAACCCAGACCTTTGTTGACACTGAAGAGAA
TGGAACGGTTAATGACCAGACTTTCCATTGCCTGCAGTCCCCACAGCGAATGAACATCCTAAACCTGGATCCTTCAGTTGCATTGGGATTTTTCTGCAAA
GAAGAAAAAGACTTTGATAACTGGTGTAGCCTTGTTCAGAAGGAAATTCTAAAGGAGAATTTAAGGATGTTTGAATTAGTTCAGAAACATCCATCACACT
GGCCTCCCTTTGTACCTCCAGCCAAGCCAGAAGTGACAACCACTGGGGCAGAATTCATTGACTCTACTGAGCAACTGGAGGAGTTTGATCTGGAGGAAGA
TTTTGAGATTCTGAGTGTGTAGAATCCTGGGAACTCAACTTGAAGGTCTGTCTTCCATCTGGCACCATAAAAACATGAACTTATTGCATAAAACTTTTCT
AGTCAGCAAGTGCCTGATATGCCAATAGCATACAAACTCAATAGCAATCATGACTGAGCCAATCACTGTTTCTCAGAAAAACAAAACAAAACAAAACAAA
TGACAGTAACCCTTCCCCGGAAAGAAATAGAACAATCATGGAGCCTAGGAGCAGAGAGATGAGGAGGAGTTCATTGCTTCCCAGCTTGTGTTATATGGCT
ACAGCAAGTCTTCAGCTGCTGCAATGAGGAAATGGGCATCTGGAAGACAAACAGCAACTCTCAGCTTGCTTCAAGAACCAGCAGATAAGAGATGGTTAAG
CTGTTCTTCACCCTTTCAGATGTGACCTCTTTTGGACTAAGCAGCAATCTGTTCTCTTGCTCAAATAATAAAGTGACTGAATCAGGGAGGAAAAGGTTCT
TGTTAAATTATTTGATTGTGTAGTTGAAGTAATTATAATTTATATCAAAACGTTTGTCAAAGAAACGATGTCAAATATACACTTCTTGATCTCCCTTCTG
TTTGCGGGGATCTTACTATTTGATGGGTCACTGTCCCCATTCTTACTGATACTTTTGTCAGATATCACCCTGTCCTTAAATCATGATCACTTAAATCAGG
GGTCAGCAAACTTTTTCTGTAAAGGGCCAGACGGGAAATATTTTGGGCTTTGCAGGCCATGCGGCCTCTGTCACATCTACTCAACTCTGCTGTTGACATG
CAAAAGCAGCAATAGACAATATGCGTGTAAATGAGTGTGGCTGTAATCCAAGAAAACTTTATTTACAAAAGCAGGTGGAGGGCTGGGTTTGGCCTGCAGG
CTGTAGCTTGCCAATCAGTGACTTAAATTGTTGATTTTTGTTTGATAAATTAAAAATAAATTGTGTTTGAAGTATA
- refseq_ATG4A.F1 (forward primer)
- refseq_ATG4A.R1 (reverse primer)
- Exon skipping and alternative donor or acceptor
Frame 0
RLCCPHRLGP*QCRTTSPRDPSDRPSVVKLPVELAQDDSWRMESVLSKYEDQITIFTDYLEEYPDTD
ELVWILGKQHLLKTDWERKTF*PETANSPAPR*
SQSLERGSSEICVFLRRHPLQRPSLSVEEGTGR
G**KGRRGS*ALWSSS*AWLHYFDWRILNSGKPG
ASN*ETFCIQTLLTQKNLSCCLI*VLVYGLHTE
GNFHQLVERALHQMLVGDVCYAVDR*CWLKPLS
VDTWEGTGAGRNKKNNPKNTNASYSAS*IEKIVA
TLSIKWHKWV*EKGNQLENGLDQIQLHRC*KNL
LYLTNGIPWLFMFQWITQWSLKISKKCAVSFP*
VLTQLVTGLPIL*LLQTRVRAPLPTAQPGNPCCS
LCPFAWA*TKSILSMLMHSKSVLRCHSL*GH*E
ENQITRIIS*DS*VTSSSSWTLIQPRPLLTLKR
MERLMTRLSIACSPHSE*TS*TWILQLHWDFSAK
KKKTLITGVALFRRKF*RRI*GCLN*FRNIHHT
GLPLYLQPSQK*QPLGQNSLTLLSNWRSLIWRK
ILRF*VCRILGTQLEGLSSIWHHKNMNLLHKTFL
VSKCLICQ*HTNSIAIMTEPITVSQKNKTKQNK
*Q*PFPGKK*NNHGA*EQRDEEEFIASQLVLYG
YSKSSAAAMRKWASGRQTATLSLLQEPADKRWLS
CSSPFQM*PLLD*AAICSLAQIIK*LNQGGKGS
C*II*LCS*SNYNLYQNVCQRNDVKYTLLDLPS
VCGDLTI*WVTVPILTDTFVRYHPVLKS*SLKSG
VSKLFL*RARREIFWALQAMRPLSHLLNSAVDM
QKQQ*TICV*MSVAVIQENFIYKSRWRAGFGLQ
AVACQSVT*IVDFCLIN*K*IVFEV
Frame 1
AFAALTDLAPSSAELQVPGILATVRP*SSCRWNWPRMTAGEWSQFYPSMKIRLLFSLTT*KNIQIQ
MSWYGS*GSSISLKQIGKERPFNLKLLTAQHPDK
ARAWKEAALRFVSF*GDILFRGPHLAWRKEQEE
ADEKAGEEVRHCGAPPEPGFIILIGEF*TVESL
ELAIRRPFVFRLF*LRKI*AVV*YKCSSMVYIQK
EIFTNWWNGPFIRCWLGMYATLWTDDAGSSPYL
*TLGKGLELGETKRTTQRIPTHPTVLLR*KRLL
LLYPSNGTNGCRRREINWRMVWTKYSCTGVKKTC
FI*RMEFLGCLCFNG*HSGH*RYQKNVPCPSLE
C*HSW*QASRFFNCFKPE*GHLCLLLSLETPAA
HCAPSPGHKPNQSCLC*CIQRVF*DATVFRGIRR
KTK*RVLFHRILR*RAHLLGPSYNPDLC*H*RE
WNG**PDFPLPAVPTANEHPKPGSFSCIGIFLQ
RRKRL**LV*PCSEGNSKGEFKDV*ISSETSITL
ASLCTSSQARSDNHWGRIH*LY*ATGGV*SGGR
F*DSECVESWELNLKVCLPSGTIKT*TYCIKLF
*SASA*YANSIQTQ*QS*LSQSLFLRKTKQNKTN
DSNPSPERNRTIMEPRSREMRRSSLLPSLCYMA
TASLQLLQ*GNGHLEDKQQLSACFKNQQIRDG*
AVLHPFRCDLFWTKQQSVLLLK**SD*IREEKVL
VKLFDCVVEVIIIYIKTFVKETMSNIHFLISLL
FAGILLFDGSLSPFLLILLSDITLSLNHDHLNQ
GSANFFCKGPDGKYFGLCRPCGLCHIYSTLLLTC
KSSNRQYACK*VWL*SKKTLFTKAGGGLGLACR
L*LANQ*LKLLIFV**IKNKLCLKY
Frame 2
PLLPSQTWPLAVQNYKSQGS*RPSVRSQVAGGIGPG*QLENGVSFIQV*RSDYYFH*LPRRISRYR
*AGMDLREAASP*NRLGKKDLLT*NC*QPSTQI
KPELGKRQL*DLCLSEETSSSEALT*RGGRNRKR
LMKRQERKLGIVELLLSLASLF*LENSEQWKAW
S*QLGDLLYSDSSNSEKSKLLSDISARLWFTYR
RKFSPIGGTGPSSDAGWGCMLRCGQMMLAQALIC
RHLGRDWSWEKQKEQPKEYQRILQCFLDRKDCC
YSIHQMAQMGVGEGKSIGEWFGPNTVAQVLKKL
ALFDEWNSLAVYVSMDNTVVIEDIKKMCRVLPLS
ADTAGDRPPDSLTASNQSKGTSAYCSAWKPLLL
IVPLRLGINQINPVYVDAFKECFKMPQSLGALG
GKPNNAYYFIGFLGDELIFLDPHTTQTFVDTEEN
GTVNDQTFHCLQSPQRMNILNLDPSVALGFFCK
EEKDFDNWCSLVQKEILKENLRMFELVQKHPSH
WPPFVPPAKPEVTTTGAEFIDSTEQLEEFDLEED
FEILSV*NPGNST*RSVFHLAP*KHELIA*NFS
SQQVPDMPIAYKLNSNHD*ANHCFSEKQNKTKQ
MTVTLPRKEIEQSWSLGAER*GGVHCFPACVIWL
QQVFSCCNEEMGIWKTNSNSQLASRTSR*EMVK
LFFTLSDVTSFGLSSNLFSCSNNKVTESGRKRF
LLNYLIV*LK*L*FISKRLSKKRCQIYTS*SPFC
LRGSYYLMGHCPHSY*YFCQISPCP*IMIT*IR
GQQTFSVKGQTGNILGFAGHAASVTSTQLCC*H
AKAAIDNMRVNECGCNPRKLYLQKQVEGWVWPAG
CSLPISDLNC*FLFDKLKINCV*SI
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