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Sequences

Sequence NM_001013746

GCCTTTGTCTCTGGCTGCAGCCGGAGCTCCTGCTCTCCTCCTCACTGCTCAGTGTCCTCTGCTCCTAGAGGCCCAGCCTCTGTGTCCCTGTGACCTGCAG
ATATTGGGAGATCCACAGCTAAGACGCCGGGACTCCCTGGAAACCTAGAAATGACTGTGCCATAACACACATTTGATGCTGAAATCCGGGATAGGGGAAC
TCCTTTGGGAGTCGTGTCCCTTAACCCCTTCCCCCGACTTCTTGAAGAGACCCACCCGCGACCTCGGTACCTCGGACCAGCTCTGTAAAAGCCCCGCCTC
TGCCTTCGGATAGGAACCACTGACATTTAAAGATGTCGCCATAGAATTCTCTCTGGAGGAGTGGCAATGCCTGGATACTGCACAGCGGGATTTATATAGG
AATGTGTTGTTAGAGAACTACAGAAACCTGGTCTTTTTGGGTATTGCTGTCTCTAAGCCATATCTGATCACCTGTCTGGAGCAAAAAAAAGAGCCCTGGA
ATATAAAAAGACATGAGATGGTAGCCAAACCCCCAGTAATGTCTTTTCATTTTGCCCAAGACCTTTGGCCAGAGCAGAACATAAAAGATTCTTTCCAGAA
AGTGACACTGAGAAGATACGGAAAATGTGAATATGAGAATTTACAGTTAAGAAAAGGCTGTAAACATGTGGATGAGTGTACGGGGCACAAAGGAGGTCAT
AATACAGTTAACCAATGTTTGACAGCTACCCCAAGCAAAATATTCCAGTGTAATAAATATGTGAAAGTCTTTGATAAATTTTCAAATTCAAATAGATATA
AGAGAAGACATACAGGAAACAAACACTTCAAATGTAAAGAATGTAGCAAATCATTTTGCGTGCTTTCACAACTAACTCAGCATAGAAGAATTCATACTAG
AGTGAATTCCTACAAATGTGAAGAATGTGGAAAAGCCTTTAACTGGTTCTCAACTCTTACTAAACATAAGAGAATTCATACTGGAGAAAAGCCCTACAAA
TGTGAAGAATGTGGCAAAGCCTTTAACCAGTCCTCACAACTTACTAGGCATAAGATAATTCATACTGAAGAGAAACCCAACAAATGTGAAGAATGTGGCA
AGGCCTTTAAACAGGCCTCACACCTTACTATACATAAAATAATTCATACTGGAGAAAAACCTTACAAATATGAAGAATGTGGCAAAGTCTTTAGCCAGTC
CTCACACCTTACTACACAAAAGATACTTCACACTGGAGAGAACCTCTACAAGTGTAAAGAATGTGGAAAAGCTTTTAACCTATTCTCAAATCTTACTAAC
CATAAGAGAATTCATGCTGGGGAGAAACCCTACAAATGTAAAGAATGTGGCAGAGCTTTTAACATATCCTCAAACCTTAATAAACAGGAGAAAATTCATA
CTGGAGGGAAACTCAACAAATGTGAAGAATGTGACAAAGCTTTTAACCGATCCTTAAAACTTACTGCACATAAGAAAATTCTAATGGAAGAGAAACCCTA
CAAATGTGAAGAATGTGGCAAAGTCTTTAACCAGTTCTCAACTCTTACTAGACATAAGATAATTCATACTGGAGAGAAACCCTACAAATGTAAAGAATGT
GGCAAAGCTTTTAACCAATCTTCAAACCTTACTGAACATAAGAAAATTCATACTGCAGAGAAATCCTATAAATGTGAAGAATGTGGCAAAGCTTTTAACC
AACACTCAAACCTAATTAACCATAGGAAAATTTATTCTGGAGAGAAACCATACAAATGTGAAGAATGTGGAAAAGCTTTTAATCGATCCTCAACCCTTAC
TAGACATAAGAAAATTCATACTGGAGAGAAACCCTACAAATGTGAAGAATGTGACAGAGCTTTTAGCCAGTCTTCAAACCTTACTGAACATAAGAAAATT
CATACTGGAGAGAAACCCTATAAATGTGAGGAATGTGGCAAAGCTTTTAACCGATTCTCAACCCTTACTAAACATAAGAGAATTCATACTGGAGAAAAAC
CCTATAAATGTGAAGAATGTGGAAAAGCCTTTAATCAATCCTATCAACTTACTAGACATAAGATAGTTCATACTAAAGAGAAACTCAACAAATGTGAAGA
ATTTGGCAAGGCCTTTAAACAGTCCTCACACCGTACTATACATAAAATTATTCATACTGGAGAGAAACCCTACAAATGTGAAGAACATGGCAAAGTTTTT
AACCAGTCCTCAAACCTTACTACACAAAAGATAATTCATACTGGAGAGAACCTCTACAAATTTGAAGAACATGGAAAAGCTTTTAACCTATTCTCAAACA
TTACTAACCATAAGATAATTTATACTGGAGAGAAACCCCACAAATGTGAAGAATGTGGAAAAGCTTATAACCGATTCTCAAACCTAACTATACATAAGAG
AATTCATACGGGAGAGAAACCTTACCAATGTGCAGAATGTGGCAAAGCCTTTAACTGCTCCTCAACCCTTAATAGACATAAGATAATTCATACTGGAGAG
AAACCTTACAAATGTAAAGAATGTGGCAAAGCTTTTAACCTATCCTCAACCCTTACTGCACATAAGAAAATTCATACTGGAGAGAAACCCTATAAATGTG
AAGAATGTGGCAAAGCTTTTAACCAATCCTCAAACCTTACTACACATAAGAAAATTCATACTTCAGAGAAACCCTACAAATGTGAAGAATGTGGCAAATC
CTTTAACCAGTTCTCATCTCTTAATATACATAAGATAATTCATACTGGAGAGAAACCCTACAAATGTGGAGATTATGGCAGAGCTTTCAACCTATCCTCA
AATCTTACTACACATAAGAAAATTCATACTGGAGAGAAACCCTACAAATGTGAGTATGGCAAAACTTAATTGATCCTACAAGCTTACTACACATAGGAAA
ATTCATACTGGAGAGAAACTACAAATGTGAAGAATGTGTTAAAGCCTTTAACAAGTCTTCAACTTTTTCTGCACACACGAGGTATTTTATACTGGTGAGA
AACCTTACAATGTAAAGAATGTGGCACAGCTTTTAACTAATCTTCAAACCTTACTGAAAGTTGAGAAAATTCGTACTGGAGAGAATCCTACAAATGTGAA
AAATGTGGCAAATCCTTTAACTGATCCTCAACTTTTACTAAACATAAGGTAATTCATACTGGAGAAAAGCCATACAAATGAGAAGAATGTGGCAATGCCT
TTAATCAGTCCTCACACCTTTCTACACATAAGATAATTTATACTGGAGAGGAACTCTATAGTTGTGAAGAATGTGGCAAAGCTTTTAACCAATCCTCATA
CCTTACTATACAGAAATTCATACTGGAGAGAAAGCCTACAATTGTGAAGAATGTGGCAAAGCTTTTAACCTTCCTTAACCCTTAACTGTAGATAAGAAAA
TTCATACTGGAAAGAAACCCTACAAATGTGAAGTCTGTGGCAAAGCTTTTAACTGATTCTCAACTCTTACTACATGTAAGAGTATTTATACTGGAAAGAA
ACCCTACAAATGTAAAAAACGTGGCAATGCCTTTAACTGGTCCTCACACCTTGCTACATATAAGACAGTTTATACTTTGAGAGAAACCCTGCAAATGTGA
ATAATGTGGCAATACTTTAAACCAATCCTCAAACCTCACTAAACATAAGATAATACTGAAAACTTTACAAACCTGAATGATGTGACAATAATTTTGACAA
CACCTCGAACTTTCTAACATAAATCATACTGGTGAAAAATCCTAGAAATGTGAAAAATATCACAAAGCCTTTAAATGGTTGTCACACTTGATTGTAGGTA
AGAATCCATACTGAAGAAAACTCCTGGAAGTGTAAAAAATGTGGCAACACTTTTAATAAATGCTCACCCCTTATTGCACAGGAAAGCATTTATACTTCAG
AAAGATTGTACAAATACAAGGAATGTGGAAAAGCCATTATTATCTGCTCAGATTTTACTCAACATTAGAGAGTTAGTACGTAATAAAAGCATTATAAATG
CAATTTTTGTCAAGAGATCTTTCAGAAAATATAAGCCTTTAAAGTGAAGAAGAGGAGCCAGTTGTGCTGGCTCACATCTGTAATGCCAGCACTTTGGGAG
GCCAAGGTGGGTGGATCACCTGAGGGCAGGAGTCTGAGACCAGCCTGGCCAACATAGCAAAACCCCATCTCTATTAAAAATTTTACAAAAATTAGCTGCG
TGTGGTGGCAGGCCCCTGTAATCCCAGCTAATTGGGAGGCTGAAGCAGGAGAATTGCTCAAACCTGGGAGGCGAAGATTGCAGTGAGCTGAAATCACACC
ACTACACTCCAGCCAAAGCAACAAGAGCAAAAACTTCGTCTCAAAAACAAAACAAAAAAAGAGTATTCATTGTGAGGACAAACAATACAAATACGAAGAG
GGTTGTAGTACCTTTACTTGTATCACAGATACTTTTGTACCCATTTTGCACTAGAGGAAAACCATGAAGCAGTTGCTCAAATGTTGTTCAACACCAGAAA
ATTTATATTGGAGAAAAGCACTGTAAATGTAATGCATTTGTGAAAACATTTTTTAAAAAACTACAGCTTAGAAAATACCAGAGGCCTCATACTAAAATAT
ATTTTTGCAAGTGAAGTAAATATAAAAATAATTTAATCCAAAATTAAGTCTATATAAATATCAGAGAATTCACAGTAGAAATATCTAAGGTACTGACACT
TCAGACATTGCTGAGTATAGAATCAGAATGCTGAGTATAGAAAATAATTTAAAACTAAAGTTGGTAGGTAAATAATGTGTATATAACTTTAAAAGAAGTA
GAATTTTTTTGTAGATGTATAATTACATTCAAATTATATTTTTTCTTGAAAAATTAGTTTTTGAAAAGCAAATGATGTAATTCAACTCAAATTCATGCTC
TTCATTCCTATTGTATTCACATGTGAAAGCATGTGATTAATTTTTCATGCATCAAAGATATGAGAAATCATTTCTGTTAGGTCGGTATTATTTATGACAC
TTTCTATGAAAGAATAAGGACATTAAAATGTAAGATGCATGATGAAAACCTGAGTGAAGAGGCTGTTTGTGTTTAACTTATAATATTGAGTGATCCATGA
AGTAGGTTTTCAGAGCAATATTCTTCTGCATTATAGTAAGAGAAAACCATTTTTAATCTTAGTTAAAATTAAAGTGAATTAGTAGTATATCATTTTACTA
TTTGTACTTTTATGTAATAAAATGCAATGTGTTTAAAAA
  • refseq_ZNF588.F2 (forward primer)
  • refseq_ZNF588.R2 (reverse primer)
  • Single exon skipping

Frame 0

AFVSGCSRSSCSPPHCSVSSAPRGPASVSL*PA
DIGRSTAKTPGLPGNLEMTVP*HTFDAEIRDRGT
PLGVVSLNPFPRLLEETHPRPRYLGPAL*KPRL
CLRIGTTDI*RCRHRILSGGVAMPGYCTAGFI*
ECVVRELQKPGLFGYCCL*AISDHLSGAKKRALE
YKKT*DGSQTPSNVFSFCPRPLARAEHKRFFPE
SDTEKIRKM*I*EFTVKKRL*TCG*VYGAQRRS
*YS*PMFDSYPKQNIPV**ICESL**IFKFK*I*
EKTYRKQTLQM*RM*QIILRAFTTNSA*KNSY*
SEFLQM*RMWKSL*LVLNSY*T*ENSYWRKALQ
M*RMWQSL*PVLTTY*A*DNSY*RETQQM*RMWQ
GL*TGLTPYYT*NNSYWRKTLQI*RMWQSL*PV
LTPYYTKDTSHWREPLQV*RMWKSF*PILKSY*
P*ENSCWGETLQM*RMWQSF*HILKP**TGENSY
WRETQQM*RM*QSF*PILKTYCT*ENSNGRETL
QM*RMWQSL*PVLNSY*T*DNSYWRETLQM*RM
WQSF*PIFKPY*T*ENSYCREIL*M*RMWQSF*P
TLKPN*P*ENLFWRETIQM*RMWKSF*SILNPY
*T*ENSYWRETLQM*RM*QSF*PVFKPY*T*EN
SYWRETL*M*GMWQSF*PILNPY*T*ENSYWRKT
L*M*RMWKSL*SILSTY*T*DSSY*RETQQM*R
IWQGL*TVLTPYYT*NYSYWRETLQM*RTWQSF
*PVLKPYYTKDNSYWREPLQI*RTWKSF*PILKH
Y*P*DNLYWRETPQM*RMWKSL*PILKPNYT*E
NSYGRETLPMCRMWQSL*LLLNP**T*DNSYWR
ETLQM*RMWQSF*PILNPYCT*ENSYWRETL*M*
RMWQSF*PILKPYYT*ENSYFRETLQM*RMWQI
L*PVLIS*YT*DNSYWRETLQMWRLWQSFQPIL
KSYYT*ENSYWRETLQM*VWQNLIDPTSLLHIGK
FILERNYKCEECVKAFNKSSTFSAHTRYFILVR
NLTM*RMWHSF*LIFKPY*KLRKFVLERILQM*
KMWQIL*LILNFY*T*GNSYWRKAIQMRRMWQCL
*SVLTPFYT*DNLYWRGTL*L*RMWQSF*PILI
PYYTEIHTGEKAYNCEECGKAFNLP*PLTVDKK
IHTGKKPYKCEVCGKAFN*FSTLTTCKSIYTGKK
PYKCKKRGNAFNWSSHLATYKTVYTLRETLQM*
IMWQYFKPILKPH*T*DNTENFTNLNDVTIILT
TPRTF*HKSYW*KILEM*KISQSL*MVVTLDCR*
ESILKKTPGSVKNVATLLINAHPLLHRKAFILQ
KDCTNTRNVEKPLLSAQILLNIRELVRNKSIIN
AIFVKRSFRKYKPLK*RRGASCAGSHL*CQHFGR
PRWVDHLRAGV*DQPGQHSKTPSLLKILQKLAA
CGGRPL*SQLIGRLKQENCSNLGGEDCSELKSH
HYTPAKATRAKTSSQKQNKKRVFIVRTNNTNTKR
VVVPLLVSQILLYPFCTRGKP*SSCSNVVQHQK
IYIGEKHCKCNAFVKTFFKKLQLRKYQRPHTKI
YFCK*SKYKNNLIQN*VYINIREFTVEISKVLTL
QTLLSIESEC*V*KII*N*SW*VNNVYITLKEV
EFFCRCIITFKLYFFLKN*FLKSK*CNSTQIHA
LHSYCIHM*KHVINFSCIKDMRNHFC*VGIIYDT
FYERIRTLKCKMHDENLSEEAVCV*LIILSDP*
SRFSEQYSSAL**EKTIFNLS*N*SELVVYHFT
ICTFM**NAMCLK

Frame 1

PLSLAAAGAPALLLTAQCPLLLEAQPLCPCDLQ
ILGDPQLRRRDSLET*K*LCHNTHLMLKSGIGE
LLWESCPLTPSPDFLKRPTRDLGTSDQLCKSPAS
AFG*EPLTFKDVAIEFSLEEWQCLDTAQRDLYR
NVLLENYRNLVFLGIAVSKPYLITCLEQKKEPW
NIKRHEMVAKPPVMSFHFAQDLWPEQNIKDSFQK
VTLRRYGKCEYENLQLRKGCKHVDECTGHKGGH
NTVNQCLTATPSKIFQCNKYVKVFDKFSNSNRY
KRRHTGNKHFKCKECSKSFCVLSQLTQHRRIHTR
VNSYKCEECGKAFNWFSTLTKHKRIHTGEKPYK
CEECGKAFNQSSQLTRHKIIHTEEKPNKCEECG
KAFKQASHLTIHKIIHTGEKPYKYEECGKVFSQS
SHLTTQKILHTGENLYKCKECGKAFNLFSNLTN
HKRIHAGEKPYKCKECGRAFNISSNLNKQEKIH
TGGKLNKCEECDKAFNRSLKLTAHKKILMEEKPY
KCEECGKVFNQFSTLTRHKIIHTGEKPYKCKEC
GKAFNQSSNLTEHKKIHTAEKSYKCEECGKAFN
QHSNLINHRKIYSGEKPYKCEECGKAFNRSSTLT
RHKKIHTGEKPYKCEECDRAFSQSSNLTEHKKI
HTGEKPYKCEECGKAFNRFSTLTKHKRIHTGEK
PYKCEECGKAFNQSYQLTRHKIVHTKEKLNKCEE
FGKAFKQSSHRTIHKIIHTGEKPYKCEEHGKVF
NQSSNLTTQKIIHTGENLYKFEEHGKAFNLFSN
ITNHKIIYTGEKPHKCEECGKAYNRFSNLTIHKR
IHTGEKPYQCAECGKAFNCSSTLNRHKIIHTGE
KPYKCKECGKAFNLSSTLTAHKKIHTGEKPYKC
EECGKAFNQSSNLTTHKKIHTSEKPYKCEECGKS
FNQFSSLNIHKIIHTGEKPYKCGDYGRAFNLSS
NLTTHKKIHTGEKPYKCEYGKT*LILQAYYT*E
NSYWRETTNVKNVLKPLTSLQLFLHTRGILYW*E
TLQCKECGTAFN*SSNLTES*ENSYWRESYKCE
KCGKSFN*SSTFTKHKVIHTGEKPYK*EECGNA
FNQSSHLSTHKIIYTGEELYSCEECGKAFNQSSY
LTIQKFILERKPTIVKNVAKLLTFLNP*L*IRK
FILERNPTNVKSVAKLLTDSQLLLHVRVFILER
NPTNVKNVAMPLTGPHTLLHIRQFIL*EKPCKCE
*CGNTLNQSSNLTKHKIILKTLQT*MM*Q*F*Q
HLELSNINHTGEKS*KCEKYHKAFKWLSHLIVG
KNPY*RKLLEV*KMWQHF**MLTPYCTGKHLYFR
KIVQIQGMWKSHYYLLRFYSTLES*YVIKAL*M
QFLSRDLSENISL*SEEEEPVVLAHICNASTLG
GQGGWIT*GQESETSLANIAKPHLY*KFYKN*LR
VVAGPCNPS*LGG*SRRIAQTWEAKIAVS*NHT
TTLQPKQQEQKLRLKNKTKKEYSL*GQTIQIRR
GL*YLYLYHRYFCTHFALEENHEAVAQMLFNTRK
FILEKSTVNVMHL*KHFLKNYSLENTRGLILKY
IFASEVNIKII*SKIKSI*ISENSQ*KYLRY*H
FRHC*V*NQNAEYRK*FKTKVGR*IMCI*L*KK*
NFFVDV*LHSNYIFS*KISF*KANDVIQLKFML
FIPIVFTCESM*LIFHASKI*EIISVRSVLFMT
LSMKE*GH*NVRCMMKT*VKRLFVFNL*Y*VIHE
VGFQSNILLHYSKRKPFLILVKIKVN**YIILL
FVLLCNKMQCV*K

Frame 2

LCLWLQPELLLSSSLLSVLCS*RPSLCVPVTCR
YWEIHS*DAGTPWKPRNDCAITHI*C*NPG*GN
SFGSRVP*PLPPTS*RDPPATSVPRTSSVKAPP
LPSDRNH*HLKMSP*NSLWRSGNAWILHSGIYIG
MCC*RTTETWSFWVLLSLSHI*SPVWSKKKSPG
I*KDMRW*PNPQ*CLFILPKTFGQSRT*KILSR
K*H*EDTENVNMRIYS*EKAVNMWMSVRGTKEVI
IQLTNV*QLPQAKYSSVINM*KSLINFQIQIDI
REDIQETNTSNVKNVANHFACFHN*LSIEEFIL
E*IPTNVKNVEKPLTGSQLLLNIREFILEKSPTN
VKNVAKPLTSPHNLLGIR*FILKRNPTNVKNVA
RPLNRPHTLLYIK*FILEKNLTNMKNVAKSLAS
PHTLLHKRYFTLERTSTSVKNVEKLLTYSQILLT
IREFMLGRNPTNVKNVAELLTYPQTLINRRKFI
LEGNSTNVKNVTKLLTDP*NLLHIRKF*WKRNP
TNVKNVAKSLTSSQLLLDIR*FILERNPTNVKNV
AKLLTNLQTLLNIRKFILQRNPINVKNVAKLLT
NTQT*LTIGKFILERNHTNVKNVEKLLIDPQPL
LDIRKFILERNPTNVKNVTELLASLQTLLNIRKF
ILERNPINVRNVAKLLTDSQPLLNIREFILEKN
PINVKNVEKPLINPINLLDIR*FILKRNSTNVK
NLARPLNSPHTVLYIKLFILERNPTNVKNMAKFL
TSPQTLLHKR*FILERTSTNLKNMEKLLTYSQT
LLTIR*FILERNPTNVKNVEKLITDSQT*LYIR
EFIRERNLTNVQNVAKPLTAPQPLIDIR*FILER
NLTNVKNVAKLLTYPQPLLHIRKFILERNPINV
KNVAKLLTNPQTLLHIRKFILQRNPTNVKNVAN
PLTSSHLLIYIR*FILERNPTNVEIMAELSTYPQ
ILLHIRKFILERNPTNVSMAKLN*SYKLTTHRK
IHTGEKLQM*RMC*SL*QVFNFFCTHEVFYTGE
KPYNVKNVAQLLTNLQTLLKVEKIRTGENPTNVK
NVANPLTDPQLLLNIR*FILEKSHTNEKNVAMP
LISPHTFLHIR*FILERNSIVVKNVAKLLTNPH
TLLYRNSYWRESLQL*RMWQSF*PSLTLNCR*EN
SYWKETLQM*SLWQSF*LILNSYYM*EYLYWKE
TLQM*KTWQCL*LVLTPCYI*DSLYFERNPANV
NNVAIL*TNPQTSLNIR*Y*KLYKPE*CDNNFDN
TSNFLT*IILVKNPRNVKNITKPLNGCHT*L*V
RIHTEENSWKCKKCGNTFNKCSPLIAQESIYTS
ERLYKYKECGKAIIICSDFTQH*RVST**KHYKC
NFCQEIFQKI*AFKVKKRSQLCWLTSVMPALWE
AKVGGSPEGRSLRPAWPT*QNPISIKNFTKISC
VWWQAPVIPANWEAEAGELLKPGRRRLQ*AEITP
LHSSQSNKSKNFVSKTKQKKSIHCEDKQYKYEE
GCSTFTCITDTFVPILH*RKTMKQLLKCCSTPE
NLYWRKAL*M*CICENIF*KTTA*KIPEASY*NI
FLQVK*I*K*FNPKLSLYKYQRIHSRNI*GTDT
SDIAEYRIRMLSIENNLKLKLVGK*CVYNFKRS
RIFL*MYNYIQIIFFLEKLVFEKQMM*FNSNSCS
SFLLYSHVKACD*FFMHQRYEKSFLLGRYYL*H
FL*KNKDIKM*DA**KPE*RGCLCLTYNIE*SM
K*VFRAIFFCIIVRENHF*S*LKLK*ISSISFYY
LYFYVIKCNVFK

Sequence NM_016220

GCCTTTGTCTCTGGCTGCAGCCGGAGCTCCTGCTCTCCTCCTCACTGCTCAGTGTCCTCTGCTCCTAGAGGCCCAGCCTCTGTGTCCCTGTGACCTGCAG
ATATTGGGAGATCCACAGCTAAGACGCCGGGACTCCCTGGAAACCTAGAAATGATGGAGTCTCGCTCTGTTGCTCAGGCTGAAGGGCAGTGGTGCGATCT
CGGCTCACTGAAACCTCCGCCTTCCGGGCTCAAGCAATTCTCCTGCCTCAACCTCGCAAGTAGCTGGGATTACAGGCATGCACCACCATGCCCAGACTGT
GCCATAACACACATTTGATGCTGAAATCCGGGATAGGGGAACTCCTTTGGGAGTCGTGTCCCTTAACCCCTTCCCCCGACTTCTTGAAGAGACCCACCCG
CGACCTCGGTACCTCGGACCAGCTCTGTAAAAGCCCCGCCTCTGCCTTCGGATAGGTCTGGCCCCACCCTGGAGTCTTGCCTCACAGAACTGATTAGAAG
AGATCAGAGTTTTGGCTGGTGCCTTTCCGGAGTGGTTGCTAACAATTCCCGAATTCCACAAGGAGATAAATGGGAAAAAAAGGAACCACTGACATTTAAA
GATGTCGCCATAGAATTCTCTCTGGAGGAGTGGCAATGCCTGGATACTGCACAGCGGGATTTATATAGGAATGTGTTGTTAGAGAACTACAGAAACCTGG
TCTTTTTGGGTATTGCTGTCTCTAAGCCATATCTGATCACCTGTCTGGAGCAAAAAAAAGAGCCCTGGAATATAAAAAGACATGAGATGGTAGCCAAACC
CCCAGTAATGTCTTTTCATTTTGCCCAAGACCTTTGGCCAGAGCAGAACATAAAAGATTCTTTCCAGAAAGTGACACTGAGAAGATACGGAAAATGTGAA
TATGAGAATTTACAGTTAAGAAAAGGCTGTAAACATGTGGATGAGTGTACGGGGCACAAAGGAGGTCATAATACAGTTAACCAATGTTTGACAGCTACCC
CAAGCAAAATATTCCAGTGTAATAAATATGTGAAAGTCTTTGATAAATTTTCAAATTCAAATAGATATAAGAGAAGACATACAGGAAACAAACACTTCAA
ATGTAAAGAATGTAGCAAATCATTTTGCGTGCTTTCACAACTAACTCAGCATAGAAGAATTCATACTAGAGTGAATTCCTACAAATGTGAAGAATGTGGA
AAAGCCTTTAACTGGTTCTCAACTCTTACTAAACATAAGAGAATTCATACTGGAGAAAAGCCCTACAAATGTGAAGAATGTGGCAAAGCCTTTAACCAGT
CCTCACAACTTACTAGGCATAAGATAATTCATACTGAAGAGAAACCCAACAAATGTGAAGAATGTGGCAAGGCCTTTAAACAGGCCTCACACCTTACTAT
ACATAAAATAATTCATACTGGAGAAAAACCTTACAAATATGAAGAATGTGGCAAAGTCTTTAGCCAGTCCTCACACCTTACTACACAAAAGATACTTCAC
ACTGGAGAGAACCTCTACAAGTGTAAAGAATGTGGAAAAGCTTTTAACCTATTCTCAAATCTTACTAACCATAAGAGAATTCATGCTGGGGAGAAACCCT
ACAAATGTAAAGAATGTGGCAGAGCTTTTAACATATCCTCAAACCTTAATAAACAGGAGAAAATTCATACTGGAGGGAAACTCAACAAATGTGAAGAATG
TGACAAAGCTTTTAACCGATCCTTAAAACTTACTGCACATAAGAAAATTCTAATGGAAGAGAAACCCTACAAATGTGAAGAATGTGGCAAAGTCTTTAAC
CAGTTCTCAACTCTTACTAGACATAAGATAATTCATACTGGAGAGAAACCCTACAAATGTAAAGAATGTGGCAAAGCTTTTAACCAATCTTCAAACCTTA
CTGAACATAAGAAAATTCATACTGCAGAGAAATCCTATAAATGTGAAGAATGTGGCAAAGCTTTTAACCAACACTCAAACCTAATTAACCATAGGAAAAT
TTATTCTGGAGAGAAACCATACAAATGTGAAGAATGTGGAAAAGCTTTTAATCGATCCTCAACCCTTACTAGACATAAGAAAATTCATACTGGAGAGAAA
CCCTACAAATGTGAAGAATGTGACAGAGCTTTTAGCCAGTCTTCAAACCTTACTGAACATAAGAAAATTCATACTGGAGAGAAACCCTATAAATGTGAGG
AATGTGGCAAAGCTTTTAACCGATTCTCAACCCTTACTAAACATAAGAGAATTCATACTGGAGAAAAACCCTATAAATGTGAAGAATGTGGAAAAGCCTT
TAATCAATCCTATCAACTTACTAGACATAAGATAGTTCATACTAAAGAGAAACTCAACAAATGTGAAGAATTTGGCAAGGCCTTTAAACAGTCCTCACAC
CGTACTATACATAAAATTATTCATACTGGAGAGAAACCCTACAAATGTGAAGAACATGGCAAAGTTTTTAACCAGTCCTCAAACCTTACTACACAAAAGA
TAATTCATACTGGAGAGAACCTCTACAAATTTGAAGAACATGGAAAAGCTTTTAACCTATTCTCAAACATTACTAACCATAAGATAATTTATACTGGAGA
GAAACCCCACAAATGTGAAGAATGTGGAAAAGCTTATAACCGATTCTCAAACCTAACTATACATAAGAGAATTCATACGGGAGAGAAACCTTACCAATGT
GCAGAATGTGGCAAAGCCTTTAACTGCTCCTCAACCCTTAATAGACATAAGATAATTCATACTGGAGAGAAACCTTACAAATGTAAAGAATGTGGCAAAG
CTTTTAACCTATCCTCAACCCTTACTGCACATAAGAAAATTCATACTGGAGAGAAACCCTATAAATGTGAAGAATGTGGCAAAGCTTTTAACCAATCCTC
AAACCTTACTACACATAAGAAAATTCATACTTCAGAGAAACCCTACAAATGTGAAGAATGTGGCAAATCCTTTAACCAGTTCTCATCTCTTAATATACAT
AAGATAATTCATACTGGAGAGAAACCCTACAAATGTGGAGATTATGGCAGAGCTTTCAACCTATCCTCAAATCTTACTACACATAAGAAAATTCATACTG
GAGAGAAACCCTACAAATGTGAGTATGGCAAAACTTAATTGATCCTACAAGCTTACTACACATAGGAAAATTCATACTGGAGAGAAACTACAAATGTGAA
GAATGTGTTAAAGCCTTTAACAAGTCTTCAACTTTTTCTGCACACACGAGGTATTTTATACTGGTGAGAAACCTTACAATGTAAAGAATGTGGCACAGCT
TTTAACTAATCTTCAAACCTTACTGAAAGTTGAGAAAATTCGTACTGGAGAGAATCCTACAAATGTGAAAAATGTGGCAAATCCTTTAACTGATCCTCAA
CTTTTACTAAACATAAGGTAATTCATACTGGAGAAAAGCCATACAAATGAGAAGAATGTGGCAATGCCTTTAATCAGTCCTCACACCTTTCTACACATAA
GATAATTTATACTGGAGAGGAACTCTATAGTTGTGAAGAATGTGGCAAAGCTTTTAACCAATCCTCATACCTTACTATACAGAAATTCATACTGGAGAGA
AAGCCTACAATTGTGAAGAATGTGGCAAAGCTTTTAACCTTCCTTAACCCTTAACTGTAGATAAGAAAATTCATACTGGAAAGAAACCCTACAAATGTGA
AGTCTGTGGCAAAGCTTTTAACTGATTCTCAACTCTTACTACATGTAAGAGTATTTATACTGGAAAGAAACCCTACAAATGTAAAAAACGTGGCAATGCC
TTTAACTGGTCCTCACACCTTGCTACATATAAGACAGTTTATACTTTGAGAGAAACCCTGCAAATGTGAATAATGTGGCAATACTTTAAACCAATCCTCA
AACCTCACTAAACATAAGATAATACTGAAAACTTTACAAACCTGAATGATGTGACAATAATTTTGACAACACCTCGAACTTTCTAACATAAATCATACTG
GTGAAAAATCCTAGAAATGTGAAAAATATCACAAAGCCTTTAAATGGTTGTCACACTTGATTGTAGGTAAGAATCCATACTGAAGAAAACTCCTGGAAGT
GTAAAAAATGTGGCAACACTTTTAATAAATGCTCACCCCTTATTGCACAGGAAAGCATTTATACTTCAGAAAGATTGTACAAATACAAGGAATGTGGAAA
AGCCATTATTATCTGCTCAGATTTTACTCAACATTAGAGAGTTAGTACGTAATAAAAGCATTATAAATGCAATTTTTGTCAAGAGATCTTTCAGAAAATA
TAAGCCTTTAAAGTGAAGAAGAGGAGCCAGTTGTGCTGGCTCACATCTGTAATGCCAGCACTTTGGGAGGCCAAGGTGGGTGGATCACCTGAGGGCAGGA
GTCTGAGACCAGCCTGGCCAACATAGCAAAACCCCATCTCTATTAAAAATTTTACAAAAATTAGCTGCGTGTGGTGGCAGGCCCCTGTAATCCCAGCTAA
TTGGGAGGCTGAAGCAGGAGAATTGCTCAAACCTGGGAGGCGAAGATTGCAGTGAGCTGAAATCACACCACTACACTCCAGCCAAAGCAACAAGAGCAAA
AACTTCGTCTCAAAAACAAAACAAAAAAAGAGTATTCATTGTGAGGACAAACAATACAAATACGAAGAGGGTTGTAGTACCTTTACTTGTATCACAGATA
CTTTTGTACCCATTTTGCACTAGAGGAAAACCATGAAGCAGTTGCTCAAATGTTGTTCAACACCAGAAAATTTATATTGGAGAAAAGCACTGTAAATGTA
ATGCATTTGTGAAAACATTTTTTAAAAAACTACAGCTTAGAAAATACCAGAGGCCTCATACTAAAATATATTTTTGCAAGTGAAGTAAATATAAAAATAA
TTTAATCCAAAATTAAGTCTATATAAATATCAGAGAATTCACAGTAGAAATATCTAAGGTACTGACACTTCAGACATTGCTGAGTATAGAATCAGAATGC
TGAGTATAGAAAATAATTTAAAACTAAAGTTGGTAGGTAAATAATGTGTATATAACTTTAAAAGAAGTAGAATTTTTTTGTAGATGTATAATTACATTCA
AATTATATTTTTTCTTGAAAAATTAGTTTTTGAAAAGCAAATGATGTAATTCAACTCAAATTCATGCTCTTCATTCCTATTGTATTCACATGTGAAAGCA
TGTGATTAATTTTTCATGCATCAAAGATATGAGAAATCATTTCTGTTAGGTCGGTATTATTTATGACACTTTCTATGAAAGAATAAGGACATTAAAATGT
AAGATGCATGATGAAAACCTGAGTGAAGAGGCTGTTTGTGTTTAACTTATAATATTGAGTGATCCATGAAGTAGGTTTTCAGAGCAATATTCTTCTGCAT
TATAGTAAGAGAAAACCATTTTTAATCTTAGTTAAAATTAAAGTGAATTAGTAGTATATCATTTTACTATTTGTACTTTTATGTAATAAAATGCAATGTG
TTTAAAAA
  • refseq_ZNF588.F2 (forward primer)
  • refseq_ZNF588.R2 (reverse primer)
  • Single exon skipping

Frame 0

AFVSGCSRSSCSPPHCSVSSAPRGPASVSL*PA
DIGRSTAKTPGLPGNLEMMESRSVAQAEGQWCDL
GSLKPPPSGLKQFSCLNLASSWDYRHAPPCPDC
AITHI*C*NPG*GNSFGSRVP*PLPPTS*RDPP
ATSVPRTSSVKAPPLPSDRSGPTLESCLTELIRR
DQSFGWCLSGVVANNSRIPQGDKWEKKEPLTFK
DVAIEFSLEEWQCLDTAQRDLYRNVLLENYRNL
VFLGIAVSKPYLITCLEQKKEPWNIKRHEMVAKP
PVMSFHFAQDLWPEQNIKDSFQKVTLRRYGKCE
YENLQLRKGCKHVDECTGHKGGHNTVNQCLTAT
PSKIFQCNKYVKVFDKFSNSNRYKRRHTGNKHFK
CKECSKSFCVLSQLTQHRRIHTRVNSYKCEECG
KAFNWFSTLTKHKRIHTGEKPYKCEECGKAFNQ
SSQLTRHKIIHTEEKPNKCEECGKAFKQASHLTI
HKIIHTGEKPYKYEECGKVFSQSSHLTTQKILH
TGENLYKCKECGKAFNLFSNLTNHKRIHAGEKP
YKCKECGRAFNISSNLNKQEKIHTGGKLNKCEEC
DKAFNRSLKLTAHKKILMEEKPYKCEECGKVFN
QFSTLTRHKIIHTGEKPYKCKECGKAFNQSSNL
TEHKKIHTAEKSYKCEECGKAFNQHSNLINHRKI
YSGEKPYKCEECGKAFNRSSTLTRHKKIHTGEK
PYKCEECDRAFSQSSNLTEHKKIHTGEKPYKCE
ECGKAFNRFSTLTKHKRIHTGEKPYKCEECGKAF
NQSYQLTRHKIVHTKEKLNKCEEFGKAFKQSSH
RTIHKIIHTGEKPYKCEEHGKVFNQSSNLTTQK
IIHTGENLYKFEEHGKAFNLFSNITNHKIIYTGE
KPHKCEECGKAYNRFSNLTIHKRIHTGEKPYQC
AECGKAFNCSSTLNRHKIIHTGEKPYKCKECGK
AFNLSSTLTAHKKIHTGEKPYKCEECGKAFNQSS
NLTTHKKIHTSEKPYKCEECGKSFNQFSSLNIH
KIIHTGEKPYKCGDYGRAFNLSSNLTTHKKIHT
GEKPYKCEYGKT*LILQAYYT*ENSYWRETTNVK
NVLKPLTSLQLFLHTRGILYW*ETLQCKECGTA
FN*SSNLTES*ENSYWRESYKCEKCGKSFN*SS
TFTKHKVIHTGEKPYK*EECGNAFNQSSHLSTHK
IIYTGEELYSCEECGKAFNQSSYLTIQKFILER
KPTIVKNVAKLLTFLNP*L*IRKFILERNPTNV
KSVAKLLTDSQLLLHVRVFILERNPTNVKNVAMP
LTGPHTLLHIRQFIL*EKPCKCE*CGNTLNQSS
NLTKHKIILKTLQT*MM*Q*F*QHLELSNINHT
GEKS*KCEKYHKAFKWLSHLIVGKNPY*RKLLEV
*KMWQHF**MLTPYCTGKHLYFRKIVQIQGMWK
SHYYLLRFYSTLES*YVIKAL*MQFLSRDLSEN
ISL*SEEEEPVVLAHICNASTLGGQGGWIT*GQE
SETSLANIAKPHLY*KFYKN*LRVVAGPCNPS*
LGG*SRRIAQTWEAKIAVS*NHTTTLQPKQQEQ
KLRLKNKTKKEYSL*GQTIQIRRGL*YLYLYHRY
FCTHFALEENHEAVAQMLFNTRKFILEKSTVNV
MHL*KHFLKNYSLENTRGLILKYIFASEVNIKI
I*SKIKSI*ISENSQ*KYLRY*HFRHC*V*NQNA
EYRK*FKTKVGR*IMCI*L*KK*NFFVDV*LHS
NYIFS*KISF*KANDVIQLKFMLFIPIVFTCES
M*LIFHASKI*EIISVRSVLFMTLSMKE*GH*NV
RCMMKT*VKRLFVFNL*Y*VIHEVGFQSNILLH
YSKRKPFLILVKIKVN**YIILLFVLLCNKMQC
V*K

Frame 1

PLSLAAAGAPALLLTAQCPLLLEAQPLCPCDLQ
ILGDPQLRRRDSLET*K*WSLALLLRLKGSGAI
SAH*NLRLPGSSNSPASTSQVAGITGMHHHAQTV
P*HTFDAEIRDRGTPLGVVSLNPFPRLLEETHP
RPRYLGPAL*KPRLCLRIGLAPPWSLASQN*LE
EIRVLAGAFPEWLLTIPEFHKEINGKKRNH*HLK
MSP*NSLWRSGNAWILHSGIYIGMCC*RTTETW
SFWVLLSLSHI*SPVWSKKKSPGI*KDMRW*PN
PQ*CLFILPKTFGQSRT*KILSRK*H*EDTENVN
MRIYS*EKAVNMWMSVRGTKEVIIQLTNV*QLP
QAKYSSVINM*KSLINFQIQIDIREDIQETNTS
NVKNVANHFACFHN*LSIEEFILE*IPTNVKNVE
KPLTGSQLLLNIREFILEKSPTNVKNVAKPLTS
PHNLLGIR*FILKRNPTNVKNVARPLNRPHTLL
YIK*FILEKNLTNMKNVAKSLASPHTLLHKRYFT
LERTSTSVKNVEKLLTYSQILLTIREFMLGRNP
TNVKNVAELLTYPQTLINRRKFILEGNSTNVKN
VTKLLTDP*NLLHIRKF*WKRNPTNVKNVAKSLT
SSQLLLDIR*FILERNPTNVKNVAKLLTNLQTL
LNIRKFILQRNPINVKNVAKLLTNTQT*LTIGK
FILERNHTNVKNVEKLLIDPQPLLDIRKFILERN
PTNVKNVTELLASLQTLLNIRKFILERNPINVR
NVAKLLTDSQPLLNIREFILEKNPINVKNVEKP
LINPINLLDIR*FILKRNSTNVKNLARPLNSPHT
VLYIKLFILERNPTNVKNMAKFLTSPQTLLHKR
*FILERTSTNLKNMEKLLTYSQTLLTIR*FILE
RNPTNVKNVEKLITDSQT*LYIREFIRERNLTNV
QNVAKPLTAPQPLIDIR*FILERNLTNVKNVAK
LLTYPQPLLHIRKFILERNPINVKNVAKLLTNP
QTLLHIRKFILQRNPTNVKNVANPLTSSHLLIYI
R*FILERNPTNVEIMAELSTYPQILLHIRKFIL
ERNPTNVSMAKLN*SYKLTTHRKIHTGEKLQM*
RMC*SL*QVFNFFCTHEVFYTGEKPYNVKNVAQL
LTNLQTLLKVEKIRTGENPTNVKNVANPLTDPQ
LLLNIR*FILEKSHTNEKNVAMPLISPHTFLHI
R*FILERNSIVVKNVAKLLTNPHTLLYRNSYWRE
SLQL*RMWQSF*PSLTLNCR*ENSYWKETLQM*
SLWQSF*LILNSYYM*EYLYWKETLQM*KTWQC
L*LVLTPCYI*DSLYFERNPANVNNVAIL*TNPQ
TSLNIR*Y*KLYKPE*CDNNFDNTSNFLT*IIL
VKNPRNVKNITKPLNGCHT*L*VRIHTEENSWK
CKKCGNTFNKCSPLIAQESIYTSERLYKYKECGK
AIIICSDFTQH*RVST**KHYKCNFCQEIFQKI
*AFKVKKRSQLCWLTSVMPALWEAKVGGSPEGR
SLRPAWPT*QNPISIKNFTKISCVWWQAPVIPAN
WEAEAGELLKPGRRRLQ*AEITPLHSSQSNKSK
NFVSKTKQKKSIHCEDKQYKYEEGCSTFTCITD
TFVPILH*RKTMKQLLKCCSTPENLYWRKAL*M*
CICENIF*KTTA*KIPEASY*NIFLQVK*I*K*
FNPKLSLYKYQRIHSRNI*GTDTSDIAEYRIRM
LSIENNLKLKLVGK*CVYNFKRSRIFL*MYNYIQ
IIFFLEKLVFEKQMM*FNSNSCSSFLLYSHVKA
CD*FFMHQRYEKSFLLGRYYL*HFL*KNKDIKM
*DA**KPE*RGCLCLTYNIE*SMK*VFRAIFFCI
IVRENHF*S*LKLK*ISSISFYYLYFYVIKCNV
FK

Frame 2

LCLWLQPELLLSSSLLSVLCS*RPSLCVPVTCR
YWEIHS*DAGTPWKPRNDGVSLCCSG*RAVVRS
RLTETSAFRAQAILLPQPRK*LGLQACTTMPRL
CHNTHLMLKSGIGELLWESCPLTPSPDFLKRPTR
DLGTSDQLCKSPASAFG*VWPHPGVLPHRTD*K
RSEFWLVPFRSGC*QFPNSTRR*MGKKGTTDI*
RCRHRILSGGVAMPGYCTAGFI*ECVVRELQKPG
LFGYCCL*AISDHLSGAKKRALEYKKT*DGSQT
PSNVFSFCPRPLARAEHKRFFPESDTEKIRKM*
I*EFTVKKRL*TCG*VYGAQRRS*YS*PMFDSYP
KQNIPV**ICESL**IFKFK*I*EKTYRKQTLQ
M*RM*QIILRAFTTNSA*KNSY*SEFLQM*RMW
KSL*LVLNSY*T*ENSYWRKALQM*RMWQSL*PV
LTTY*A*DNSY*RETQQM*RMWQGL*TGLTPYY
T*NNSYWRKTLQI*RMWQSL*PVLTPYYTKDTS
HWREPLQV*RMWKSF*PILKSY*P*ENSCWGETL
QM*RMWQSF*HILKP**TGENSYWRETQQM*RM
*QSF*PILKTYCT*ENSNGRETLQM*RMWQSL*
PVLNSY*T*DNSYWRETLQM*RMWQSF*PIFKPY
*T*ENSYCREIL*M*RMWQSF*PTLKPN*P*EN
LFWRETIQM*RMWKSF*SILNPY*T*ENSYWRE
TLQM*RM*QSF*PVFKPY*T*ENSYWRETL*M*G
MWQSF*PILNPY*T*ENSYWRKTL*M*RMWKSL
*SILSTY*T*DSSY*RETQQM*RIWQGL*TVLT
PYYT*NYSYWRETLQM*RTWQSF*PVLKPYYTKD
NSYWREPLQI*RTWKSF*PILKHY*P*DNLYWR
ETPQM*RMWKSL*PILKPNYT*ENSYGRETLPM
CRMWQSL*LLLNP**T*DNSYWRETLQM*RMWQS
F*PILNPYCT*ENSYWRETL*M*RMWQSF*PIL
KPYYT*ENSYFRETLQM*RMWQIL*PVLIS*YT
*DNSYWRETLQMWRLWQSFQPILKSYYT*ENSYW
RETLQM*VWQNLIDPTSLLHIGKFILERNYKCE
ECVKAFNKSSTFSAHTRYFILVRNLTM*RMWHS
F*LIFKPY*KLRKFVLERILQM*KMWQIL*LILN
FY*T*GNSYWRKAIQMRRMWQCL*SVLTPFYT*
DNLYWRGTL*L*RMWQSF*PILIPYYTEIHTGE
KAYNCEECGKAFNLP*PLTVDKKIHTGKKPYKCE
VCGKAFN*FSTLTTCKSIYTGKKPYKCKKRGNA
FNWSSHLATYKTVYTLRETLQM*IMWQYFKPIL
KPH*T*DNTENFTNLNDVTIILTTPRTF*HKSYW
*KILEM*KISQSL*MVVTLDCR*ESILKKTPGS
VKNVATLLINAHPLLHRKAFILQKDCTNTRNVE
KPLLSAQILLNIRELVRNKSIINAIFVKRSFRKY
KPLK*RRGASCAGSHL*CQHFGRPRWVDHLRAG
V*DQPGQHSKTPSLLKILQKLAACGGRPL*SQL
IGRLKQENCSNLGGEDCSELKSHHYTPAKATRAK
TSSQKQNKKRVFIVRTNNTNTKRVVVPLLVSQI
LLYPFCTRGKP*SSCSNVVQHQKIYIGEKHCKC
NAFVKTFFKKLQLRKYQRPHTKIYFCK*SKYKNN
LIQN*VYINIREFTVEISKVLTLQTLLSIESEC
*V*KII*N*SW*VNNVYITLKEVEFFCRCIITF
KLYFFLKN*FLKSK*CNSTQIHALHSYCIHM*KH
VINFSCIKDMRNHFC*VGIIYDTFYERIRTLKC
KMHDENLSEEAVCV*LIILSDP*SRFSEQYSSA
L**EKTIFNLS*N*SELVVYHFTICTFM**NAMC
LK

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