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Sequences

Sequence NM_001037969

CACCTGTCATTCGTTCGTCCTCAGTGCAGGGCAACAGATTCTCCATCTTGGGCGAAGGGCCACTGTCCCTTCATGCTGTCGGAACTCCAGGATGGGAAGA
GCAAGATTGTGCAGAAGGCAGCCCCAGAAGTCCAGGAAGATGTGAAGGCTTTCAAGACAGATCTGCCTAGCTCCCTGGTCTCAGTCAGCCTAAGGAAGCC
ATTTTCCGGTCCCCAGGAGCAGGAGCAGATCTCTGGGAAGGTCACACACCTGATTCAGAACAATATGCCTGGAAACTATGTCTTCAGTTATGACCAGTTT
TTCAGGGACAAGATCATGGAGAAGAAACAGGATCACACCTACCGTGTGTTCAAGACTGTGAACCGCTGGGCTGATGCATATCCCTTTGCCCAACATTTCT
CTGAGGCATCTGTGGCCTCAAAGGATGTGTCCGTCTGGTGTAGTAATGATTACCTGGGCATGAGCCGACACCCTCAGGTCTTGCAAGCCACACAGGAGAC
CCTGCAGCGTCATGGTGCTGGAGCTGGTGGCACCCGCAACATCTCAGGCACCAGTAAGTTTCATGTGGAGCTTGAGCAGGAGCTGGCTGAGCTGCACCAG
AAGGACTCAGCCCTGCTCTTCTCCTCCTGCTTTGTTGCCAATGACTCTACTCTCTTCACCTTGGCCAAGATCCTGCCAGGGTGCGAGATTTACTCAGACG
CAGGCAACCATGCTTCCATGATCCAAGGTATCCGTAACAGTGGAGCAGCCAAGTTTGTCTTCAGGCACAATGACCCTGACCACCTAAAGAAACTTCTAGA
GAAGTCTAACCCTAAGATACCCAAAATTGTGGCCTTTGAGACTGTCCACTCCATGGATGGTGCCATCTGTCCCCTCGAGGAGTTGTGTGATGTGTCCCAC
CAGTATGGGGCCCTGACCTTCGTGGATGAGGTCCATGCTGTAGGACTGTATGGGTCCCGGGGCGCTGGGATTGGGGAGCGTGATGGAATTATGCATAAGA
TTGACATCATCTCTGGAACTCTTGGCAAGGCCTTTGGCTGTGTGGGCGGCTACATTGCCAGCACCCGTGACTTGGTGGACATGGTGCGCTCCTATGCTGC
AGGCTTCATCTTTACCACTTCTCTGCCCCCCATGGTGCTCTCTGGAGCTCTAGAATCTGTGCGGCTGCTCAAGGGAGAGGAGGGCCAAGCCCTGAGGCGA
GCCCACCAGCGCAATGTCAAGCACATGCGCCAGCTACTCATGGACAGGGGCCTTCCTGTCATCCCCTGCCCCAGCCACATCATCCCCATCCGGGTGGGCA
ATGCAGCACTCAACAGCAAGCTCTGTGATCTCCTGCTCTCCAAGCATGGCATCTATGTGCAGGCCATCAACTACCCAACTGTCCCCCGGGGTGAAGAGCT
CCTGCGCTTGGCACCCTCCCCCCACCACAGCCCTCAGATGATGGAAGATTTTGTGGAGAAGCTGCTGCTGGCTTGGACTGCGGTGGGGCTGCCCCTCCAG
GATGTGTCTGTGGCTGCCTGCAATTTCTGTCGCCGTCCTGTACACTTTGAGCTCATGAGTGAGTGGGAACGTTCCTACTTCGGGAACATGGGGCCCCAGT
ATGTCACCACCTATGCCTGAGAAGCCAGCTGCCTAGGATTCACACCCCACCTGCGCTTCACTTGGGTCCAGGCCTACTCCTGTCTTCTGCTTTGTTGTGT
GCCTCTAGCTGAATTGAGCCTAAAAATAAAGCACAAACCACAGCA
  • refseq_ALAS2.F1 (forward primer)
  • refseq_ALAS2.R1 (reverse primer)
  • Single exon skipping

Frame 0

HLSFVRPQCRATDSPSWAKGHCPFMLSELQDGK
SKIVQKAAPEVQEDVKAFKTDLPSSLVSVSLRKP
FSGPQEQEQISGKVTHLIQNNMPGNYVFSYDQF
FRDKIMEKKQDHTYRVFKTVNRWADAYPFAQHF
SEASVASKDVSVWCSNDYLGMSRHPQVLQATQET
LQRHGAGAGGTRNISGTSKFHVELEQELAELHQ
KDSALLFSSCFVANDSTLFTLAKILPGCEIYSD
AGNHASMIQGIRNSGAAKFVFRHNDPDHLKKLLE
KSNPKIPKIVAFETVHSMDGAICPLEELCDVSH
QYGALTFVDEVHAVGLYGSRGAGIGERDGIMHK
IDIISGTLGKAFGCVGGYIASTRDLVDMVRSYAA
GFIFTTSLPPMVLSGALESVRLLKGEEGQALRR
AHQRNVKHMRQLLMDRGLPVIPCPSHIIPIRVG
NAALNSKLCDLLLSKHGIYVQAINYPTVPRGEEL
LRLAPSPHHSPQMMEDFVEKLLLAWTAVGLPLQ
DVSVAACNFCRRPVHFELMSEWERSYFGNMGPQ
YVTTYA*EASCLGFTPHLRFTWVQAYSCLLLCCV
PLAELSLKIKHKPQ

Frame 1

TCHSFVLSAGQQILHLGRRATVPSCCRNSRMGR
ARLCRRQPQKSRKM*RLSRQICLAPWSQSA*GS
HFPVPRSRSRSLGRSHT*FRTICLETMSSVMTSF
SGTRSWRRNRITPTVCSRL*TAGLMHIPLPNIS
LRHLWPQRMCPSGVVMITWA*ADTLRSCKPHRR
PCSVMVLELVAPATSQAPVSFMWSLSRSWLSCTR
RTQPCSSPPALLPMTLLSSPWPRSCQGARFTQT
QATMLP*SKVSVTVEQPSLSSGTMTLTT*RNF*
RSLTLRYPKLWPLRLSTPWMVPSVPSRSCVMCPT
SMGP*PSWMRSML*DCMGPGALGLGSVMELCIR
LTSSLELLARPLAVWAATLPAPVTWWTWCAPML
QASSLPLLCPPWCSLEL*NLCGCSRERRAKP*GE
PTSAMSSTCASYSWTGAFLSSPAPATSSPSGWA
MQHSTASSVISCSPSMASMCRPSTTQLSPGVKS
SCAWHPPPTTALR*WKILWRSCCWLGLRWGCPSR
MCLWLPAISVAVLYTLSS*VSGNVPTSGTWGPS
MSPPMPEKPAA*DSHPTCASLGSRPTPVFCFVV
CL*LN*A*K*STNHS

Frame 2

PVIRSSSVQGNRFSILGEGPLSLHAVGTPGWEE
QDCAEGSPRSPGRCEGFQDRSA*LPGLSQPKEA
IFRSPGAGADLWEGHTPDSEQYAWKLCLQL*PV
FQGQDHGEETGSHLPCVQDCEPLG*CISLCPTFL
*GICGLKGCVRLV***LPGHEPTPSGLASHTGD
PAASWCWSWWHPQHLRHQ*VSCGA*AGAG*AAP
EGLSPALLLLLCCQ*LYSLHLGQDPARVRDLLRR
RQPCFHDPRYP*QWSSQVCLQAQ*P*PPKETSR
EV*P*DTQNCGL*DCPLHGWCHLSPRGVV*CVP
PVWGPDLRG*GPCCRTVWVPGRWDWGA*WNYA*D
*HHLWNSWQGLWLCGRLHCQHP*LGGHGALLCC
RLHLYHFSAPHGALWSSRICAAAQGRGGPSPEA
SPPAQCQAHAPATHGQGPSCHPLPQPHHPHPGGQ
CSTQQQAL*SPALQAWHLCAGHQLPNCPPG*RA
PALGTLPPPQPSDDGRFCGEAAAGLDCGGAAPP
GCVCGCLQFLSPSCTL*AHE*VGTFLLREHGAPV
CHHLCLRSQLPRIHTPPALHLGPGLLLSSALLC
ASS*IEPKNKAQTTA

Sequence NM_000032

CACCTGTCATTCGTTCGTCCTCAGTGCAGGGCAACAGGACTTTAGGTTCAAGATGGTGACTGCAGCCATGCTGCTACAGTGCTGCCCAGTGCTTGCCCGG
GGCCCCACAAGCCTCCTAGGCAAGGTGGTTAAGACTCACCAGTTCCTGTTTGGTATTGGACGCTGTCCCATCCTGGCTACCCAAGGACCAAACTGTTCTC
AAATCCACCTTAAGGCAACAAAGGCTGGAGGAGATTCTCCATCTTGGGCGAAGGGCCACTGTCCCTTCATGCTGTCGGAACTCCAGGATGGGAAGAGCAA
GATTGTGCAGAAGGCAGCCCCAGAAGTCCAGGAAGATGTGAAGGCTTTCAAGACAGATCTGCCTAGCTCCCTGGTCTCAGTCAGCCTAAGGAAGCCATTT
TCCGGTCCCCAGGAGCAGGAGCAGATCTCTGGGAAGGTCACACACCTGATTCAGAACAATATGCCTGGAAACTATGTCTTCAGTTATGACCAGTTTTTCA
GGGACAAGATCATGGAGAAGAAACAGGATCACACCTACCGTGTGTTCAAGACTGTGAACCGCTGGGCTGATGCATATCCCTTTGCCCAACATTTCTCTGA
GGCATCTGTGGCCTCAAAGGATGTGTCCGTCTGGTGTAGTAATGATTACCTGGGCATGAGCCGACACCCTCAGGTCTTGCAAGCCACACAGGAGACCCTG
CAGCGTCATGGTGCTGGAGCTGGTGGCACCCGCAACATCTCAGGCACCAGTAAGTTTCATGTGGAGCTTGAGCAGGAGCTGGCTGAGCTGCACCAGAAGG
ACTCAGCCCTGCTCTTCTCCTCCTGCTTTGTTGCCAATGACTCTACTCTCTTCACCTTGGCCAAGATCCTGCCAGGGTGCGAGATTTACTCAGACGCAGG
CAACCATGCTTCCATGATCCAAGGTATCCGTAACAGTGGAGCAGCCAAGTTTGTCTTCAGGCACAATGACCCTGACCACCTAAAGAAACTTCTAGAGAAG
TCTAACCCTAAGATACCCAAAATTGTGGCCTTTGAGACTGTCCACTCCATGGATGGTGCCATCTGTCCCCTCGAGGAGTTGTGTGATGTGTCCCACCAGT
ATGGGGCCCTGACCTTCGTGGATGAGGTCCATGCTGTAGGACTGTATGGGTCCCGGGGCGCTGGGATTGGGGAGCGTGATGGAATTATGCATAAGATTGA
CATCATCTCTGGAACTCTTGGCAAGGCCTTTGGCTGTGTGGGCGGCTACATTGCCAGCACCCGTGACTTGGTGGACATGGTGCGCTCCTATGCTGCAGGC
TTCATCTTTACCACTTCTCTGCCCCCCATGGTGCTCTCTGGAGCTCTAGAATCTGTGCGGCTGCTCAAGGGAGAGGAGGGCCAAGCCCTGAGGCGAGCCC
ACCAGCGCAATGTCAAGCACATGCGCCAGCTACTCATGGACAGGGGCCTTCCTGTCATCCCCTGCCCCAGCCACATCATCCCCATCCGGGTGGGCAATGC
AGCACTCAACAGCAAGCTCTGTGATCTCCTGCTCTCCAAGCATGGCATCTATGTGCAGGCCATCAACTACCCAACTGTCCCCCGGGGTGAAGAGCTCCTG
CGCTTGGCACCCTCCCCCCACCACAGCCCTCAGATGATGGAAGATTTTGTGGAGAAGCTGCTGCTGGCTTGGACTGCGGTGGGGCTGCCCCTCCAGGATG
TGTCTGTGGCTGCCTGCAATTTCTGTCGCCGTCCTGTACACTTTGAGCTCATGAGTGAGTGGGAACGTTCCTACTTCGGGAACATGGGGCCCCAGTATGT
CACCACCTATGCCTGAGAAGCCAGCTGCCTAGGATTCACACCCCACCTGCGCTTCACTTGGGTCCAGGCCTACTCCTGTCTTCTGCTTTGTTGTGTGCCT
CTAGCTGAATTGAGCCTAAAAATAAAGCACAAACCACAGCA
  • refseq_ALAS2.F1 (forward primer)
  • refseq_ALAS2.R1 (reverse primer)
  • Single exon skipping

Frame 0

HLSFVRPQCRATGL*VQDGDCSHAATVLPSACP
GPHKPPRQGG*DSPVPVWYWTLSHPGYPRTKLFS
NPP*GNKGWRRFSILGEGPLSLHAVGTPGWEEQ
DCAEGSPRSPGRCEGFQDRSA*LPGLSQPKEAI
FRSPGAGADLWEGHTPDSEQYAWKLCLQL*PVFQ
GQDHGEETGSHLPCVQDCEPLG*CISLCPTFL*
GICGLKGCVRLV***LPGHEPTPSGLASHTGDP
AASWCWSWWHPQHLRHQ*VSCGA*AGAG*AAPEG
LSPALLLLLCCQ*LYSLHLGQDPARVRDLLRRR
QPCFHDPRYP*QWSSQVCLQAQ*P*PPKETSRE
V*P*DTQNCGL*DCPLHGWCHLSPRGVV*CVPPV
WGPDLRG*GPCCRTVWVPGRWDWGA*WNYA*D*
HHLWNSWQGLWLCGRLHCQHP*LGGHGALLCCR
LHLYHFSAPHGALWSSRICAAAQGRGGPSPEASP
PAQCQAHAPATHGQGPSCHPLPQPHHPHPGGQC
STQQQAL*SPALQAWHLCAGHQLPNCPPG*RAP
ALGTLPPPQPSDDGRFCGEAAAGLDCGGAAPPGC
VCGCLQFLSPSCTL*AHE*VGTFLLREHGAPVC
HHLCLRSQLPRIHTPPALHLGPGLLLSSALLCA
SS*IEPKNKAQTTA

Frame 1

TCHSFVLSAGQQDFRFKMVTAAMLLQCCPVLAR
GPTSLLGKVVKTHQFLFGIGRCPILATQGPNCS
QIHLKATKAGGDSPSWAKGHCPFMLSELQDGKSK
IVQKAAPEVQEDVKAFKTDLPSSLVSVSLRKPF
SGPQEQEQISGKVTHLIQNNMPGNYVFSYDQFF
RDKIMEKKQDHTYRVFKTVNRWADAYPFAQHFSE
ASVASKDVSVWCSNDYLGMSRHPQVLQATQETL
QRHGAGAGGTRNISGTSKFHVELEQELAELHQK
DSALLFSSCFVANDSTLFTLAKILPGCEIYSDAG
NHASMIQGIRNSGAAKFVFRHNDPDHLKKLLEK
SNPKIPKIVAFETVHSMDGAICPLEELCDVSHQ
YGALTFVDEVHAVGLYGSRGAGIGERDGIMHKID
IISGTLGKAFGCVGGYIASTRDLVDMVRSYAAG
FIFTTSLPPMVLSGALESVRLLKGEEGQALRRA
HQRNVKHMRQLLMDRGLPVIPCPSHIIPIRVGNA
ALNSKLCDLLLSKHGIYVQAINYPTVPRGEELL
RLAPSPHHSPQMMEDFVEKLLLAWTAVGLPLQD
VSVAACNFCRRPVHFELMSEWERSYFGNMGPQYV
TTYA*EASCLGFTPHLRFTWVQAYSCLLLCCVP
LAELSLKIKHKPQ

Frame 2

PVIRSSSVQGNRTLGSRW*LQPCCYSAAQCLPG
APQAS*ARWLRLTSSCLVLDAVPSWLPKDQTVL
KSTLRQQRLEEILHLGRRATVPSCCRNSRMGRA
RLCRRQPQKSRKM*RLSRQICLAPWSQSA*GSHF
PVPRSRSRSLGRSHT*FRTICLETMSSVMTSFS
GTRSWRRNRITPTVCSRL*TAGLMHIPLPNISL
RHLWPQRMCPSGVVMITWA*ADTLRSCKPHRRPC
SVMVLELVAPATSQAPVSFMWSLSRSWLSCTRR
TQPCSSPPALLPMTLLSSPWPRSCQGARFTQTQ
ATMLP*SKVSVTVEQPSLSSGTMTLTT*RNF*RS
LTLRYPKLWPLRLSTPWMVPSVPSRSCVMCPTS
MGP*PSWMRSML*DCMGPGALGLGSVMELCIRL
TSSLELLARPLAVWAATLPAPVTWWTWCAPMLQA
SSLPLLCPPWCSLEL*NLCGCSRERRAKP*GEP
TSAMSSTCASYSWTGAFLSSPAPATSSPSGWAM
QHSTASSVISCSPSMASMCRPSTTQLSPGVKSSC
AWHPPPTTALR*WKILWRSCCWLGLRWGCPSRM
CLWLPAISVAVLYTLSS*VSGNVPTSGTWGPSM
SPPMPEKPAA*DSHPTCASLGSRPTPVFCFVVCL
*LN*A*K*STNHS

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