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Sequences

Sequence NM_153326

AGCGCCACCTGCCTCATTGTGCCCAGGAGTTCTCCAAACCCGCGCTGCGGAGTGAGTGACCAAGTTCCGGCCAGTTCGACCTCGAGGATCCAGAGGTGGA GACGGTACTACCTCCCAGCTCTGTTTTCCATCCCCTTCAGGTCCTTCCTCGGGAGGCGGCGAAGGCGGTCCACCCTGCGCGTGATCCTTTATGCCCGGCC CCTGCCCCTCCCTCCGGGTGGAACTTCCCCCTCACCGCCAGACTTAAGCTGAGGATCGTTGGATCTCTGGCGGGGTGCAGAACTGAGCCCAGGCCACAGT ACCCTATTCACGCTCTGTGCTTGTGCCAAGGGGGCAATGGCGGCTTCCTGTGTTCTACTGCACACTGGGCAGAAGATGCCTCTGATTGGTCTGGGTACCT GGAAGAGTGAGCCTGGTCAGGTAAAAGCAGCTGTTAAGTATGCCCTTAGCGTAGGCTACCGCCACATTGATTGTGCTGCTATCTACGGCAATGAGCCTGA GATTGGGGAGGCCCTGAAGGAGGACGTGGGACCAGGCAAGGCGGTGCCTCGGGAGGAGCTGTTTGTGACATCCAAGCTGTGGAACACCAAGCACCACCCC GAGGATGTGGAGCCTGCCCTCCGGAAGACTCTGGCTGACCTCCAGCTGGAGTATCTGGACCTGTACCTGATGCACTGGCCTTATGCCTTTGAGCGGGGAG ACAACCCCTTCCCCAAGAATGCTGATGGGACTATATGCTACGACTCCACCCACTACAAGGAGACTTGGAAGGCTCTGGAGGCACTGGTGGCTAAGGGGCT GGTGCAGGCGCTGGGCCTGTCCAACTTCAACAGTCGGCAGATTGATGACATACTCAGTGTGGCCTCCGTGCGTCCAGCTGTCTTGCAGGTGGAATGCCAC CCATACTTGGCTCAAAATGAGCTAATTGCCCACTGCCAAGCACGTGGCCTGGAGGTAACTGCTTATAGCCCTTTGGGCTCCTCTGATCGTGCATGGCGTG ATCCTGATGAGCCTGTCCTGCTGGAGGAACCAGTAGTCCTGGCATTGGCTGAAAAGTATGGCCGATCTCCAGCTCAGATCTTGCTCAGGTGGCAGGTCCA GCGGAAAGTGATCTGCATCCCCAAAAGTATCACTCCTTCTCGAATCCTTCAGAACATCAAGGTGTTTGACTTCACCTTTAGCCCAGAAGAGATGAAGCAG CTAAATGCCCTGAACAAAAATTGGAGATATATTGTGCCTATGCTTACGGTGGATGGGAAGAGAGTCCCAAGGGATGCAGGGCATCCTCTGTACCCCTTTA ATGACCCGTACTGAGACCACAGCTTCTTGGCCTCCCTTCCAGCTCTGCAGCTAATGAGGTCCTGCCACAACGGAAAGAGGGAGTTAATAAAGCCATTGGA GCATCCA

refseq_AKR1A1.F2 (forward primer)
refseq_AKR1A1.R2 (reverse primer)
Single exon skipping

Frame 0

SATCLIVPRSSPNPRCGVSDQVPASSTSRIQRW RRYYLPALFSIPFRSFLGRRRRRSTLRVILYARP LPLPPGGTSPSPPDLS*GSLDLWRGAELSPGHS TLFTLCACAKGAMAASCVLLHTGQKMPLIGLGT WKSEPGQVKAAVKYALSVGYRHIDCAAIYGNEPE IGEALKEDVGPGKAVPREELFVTSKLWNTKHHP EDVEPALRKTLADLQLEYLDLYLMHWPYAFERG DNPFPKNADGTICYDSTHYKETWKALEALVAKGL VQALGLSNFNSRQIDDILSVASVRPAVLQVECH PYLAQNELIAHCQARGLEVTAYSPLGSSDRAWR DPDEPVLLEEPVVLALAEKYGRSPAQILLRWQVQ RKVICIPKSITPSRILQNIKVFDFTFSPEEMKQ LNALNKNWRYIVPMLTVDGKRVPRDAGHPLYPF NDPY*DHSFLASLPALQLMRSCHNGKRELIKPLE HP

Frame 1

APPASLCPGVLQTRAAE*VTKFRPVRPRGSRGG DGTTSQLCFPSPSGPSSGGGEGGPPCA*SFMPG PCPSLRVELPPHRQT*AEDRWISGGVQN*AQATV PYSRSVLVPRGQWRLPVFYCTLGRRCL*LVWVP GRVSLVR*KQLLSMPLA*ATATLIVLLSTAMSL RLGRP*RRTWDQARRCLGRSCL*HPSCGTPSTTP RMWSLPSGRLWLTSSWSIWTCT*CTGLMPLSGE TTPSPRMLMGLYATTPPTTRRLGRLWRHWWLRG WCRRWACPTSTVGRLMTYSVWPPCVQLSCRWNAT HTWLKMS*LPTAKHVAWR*LLIALWAPLIVHGV ILMSLSCWRNQ*SWHWLKSMADLQLRSCSGGRS SGK*SASPKVSLLLESFRTSRCLTSPLAQKR*SS *MP*TKIGDILCLCLRWMGRESQGMQGILCTPL MTRTETTASWPPFQLCS**GPATTERGS**SHW SI

Frame 2

RHLPHCAQEFSKPALRSE*PSSGQFDLEDPEVE TVLPPSSVFHPLQVLPREAAKAVHPARDPLCPA PAPPSGWNFPLTARLKLRIVGSLAGCRTEPRPQ YPIHALCLCQGGNGGFLCSTAHWAEDASDWSGYL EE*AWSGKSSC*VCP*RRLPPH*LCCYLRQ*A* DWGGPEGGRGTRQGGASGGAVCDIQAVEHQAPP RGCGACPPEDSG*PPAGVSGPVPDALALCL*AGR QPLPQEC*WDYMLRLHPLQGDLEGSGGTGG*GA GAGAGPVQLQQSAD**HTQCGLRASSCLAGGMP PILGSK*ANCPLPSTWPGGNCL*PFGLL*SCMA* S**ACPAGGTSSPGIG*KVWPISSSDLAQVAGP AESDLHPQKYHSFSNPSEHQGV*LHL*PRRDEA AKCPEQKLEIYCAYAYGGWEESPKGCRASSVPL* *PVLRPQLLGLPSSSAANEVLPQRKEGVNKAIG AS

Sequence NM_006066

AGCGCCACCTGCCTCATTGTGCCCAGGAGTTCTCCAAACCCGCGCTGCGGAGTGAGTGACCAAGTTCCGGCCAGTTCGACCTCGAGGATCCAGAGGTGGA GACGGTACTACCTCCCAGCTCTGTTTTCCATCCCCTTCAGGTCCTTCCTCGGGAGGCGGCGAAGGCGGTCCACCCTGCGCGTGATCCTTTATGCCCGGCC CCTGCCCCTCCCTCCGGGTGGAACTTCCCCCTCACCGCCAGACTTAAGCTGAGGATCGTTGGATCTCTGGCGGGGTGCAGAACTGAGCCCAGGCCACAGT ACCCTATTCACGCTCTGTGCTTGTGCCAAGGTTTCAAGTGATCCTCCCGCCTCAGCCTGCCCAGGTGCTGAGATTACATGTATGAGCCACTGCACCTGGA AAGGAGCCAGAAATGTGAAGTGCTAGCTGAAGGATGAGCAGCAGCTAGCCAGGCAAAGGGGGCAATGGCGGCTTCCTGTGTTCTACTGCACACTGGGCAG AAGATGCCTCTGATTGGTCTGGGTACCTGGAAGAGTGAGCCTGGTCAGGTAAAAGCAGCTGTTAAGTATGCCCTTAGCGTAGGCTACCGCCACATTGATT GTGCTGCTATCTACGGCAATGAGCCTGAGATTGGGGAGGCCCTGAAGGAGGACGTGGGACCAGGCAAGGCGGTGCCTCGGGAGGAGCTGTTTGTGACATC CAAGCTGTGGAACACCAAGCACCACCCCGAGGATGTGGAGCCTGCCCTCCGGAAGACTCTGGCTGACCTCCAGCTGGAGTATCTGGACCTGTACCTGATG CACTGGCCTTATGCCTTTGAGCGGGGAGACAACCCCTTCCCCAAGAATGCTGATGGGACTATATGCTACGACTCCACCCACTACAAGGAGACTTGGAAGG CTCTGGAGGCACTGGTGGCTAAGGGGCTGGTGCAGGCGCTGGGCCTGTCCAACTTCAACAGTCGGCAGATTGATGACATACTCAGTGTGGCCTCCGTGCG TCCAGCTGTCTTGCAGGTGGAATGCCACCCATACTTGGCTCAAAATGAGCTAATTGCCCACTGCCAAGCACGTGGCCTGGAGGTAACTGCTTATAGCCCT TTGGGCTCCTCTGATCGTGCATGGCGTGATCCTGATGAGCCTGTCCTGCTGGAGGAACCAGTAGTCCTGGCATTGGCTGAAAAGTATGGCCGATCTCCAG CTCAGATCTTGCTCAGGTGGCAGGTCCAGCGGAAAGTGATCTGCATCCCCAAAAGTATCACTCCTTCTCGAATCCTTCAGAACATCAAGGTGTTTGACTT CACCTTTAGCCCAGAAGAGATGAAGCAGCTAAATGCCCTGAACAAAAATTGGAGATATATTGTGCCTATGCTTACGGTGGATGGGAAGAGAGTCCCAAGG GATGCAGGGCATCCTCTGTACCCCTTTAATGACCCGTACTGAGACCACAGCTTCTTGGCCTCCCTTCCAGCTCTGCAGCTAATGAGGTCCTGCCACAACG GAAAGAGGGAGTTAATAAAGCCATTGGAGCATCCA

refseq_AKR1A1.F2 (forward primer)
refseq_AKR1A1.R2 (reverse primer)
Single exon skipping

Frame 0

SATCLIVPRSSPNPRCGVSDQVPASSTSRIQRW RRYYLPALFSIPFRSFLGRRRRRSTLRVILYARP LPLPPGGTSPSPPDLS*GSLDLWRGAELSPGHS TLFTLCACAKVSSDPPASACPGAEITCMSHCTW KGARNVKC*LKDEQQLARQRGQWRLPVFYCTLGR RCL*LVWVPGRVSLVR*KQLLSMPLA*ATATLI VLLSTAMSLRLGRP*RRTWDQARRCLGRSCL*H PSCGTPSTTPRMWSLPSGRLWLTSSWSIWTCT*C TGLMPLSGETTPSPRMLMGLYATTPPTTRRLGR LWRHWWLRGWCRRWACPTSTVGRLMTYSVWPPC VQLSCRWNATHTWLKMS*LPTAKHVAWR*LLIAL WAPLIVHGVILMSLSCWRNQ*SWHWLKSMADLQ LRSCSGGRSSGK*SASPKVSLLLESFRTSRCLT SPLAQKR*SS*MP*TKIGDILCLCLRWMGRESQG MQGILCTPLMTRTETTASWPPFQLCS**GPATT ERGS**SHWSI

Frame 1

APPASLCPGVLQTRAAE*VTKFRPVRPRGSRGG DGTTSQLCFPSPSGPSSGGGEGGPPCA*SFMPG PCPSLRVELPPHRQT*AEDRWISGGVQN*AQATV PYSRSVLVPRFQVILPPQPAQVLRLHV*ATAPG KEPEM*SAS*RMSSS*PGKGGNGGFLCSTAHWA EDASDWSGYLEE*AWSGKSSC*VCP*RRLPPH*L CCYLRQ*A*DWGGPEGGRGTRQGGASGGAVCDI QAVEHQAPPRGCGACPPEDSG*PPAGVSGPVPD ALALCL*AGRQPLPQEC*WDYMLRLHPLQGDLEG SGGTGG*GAGAGAGPVQLQQSAD**HTQCGLRA SSCLAGGMPPILGSK*ANCPLPSTWPGGNCL*P FGLL*SCMA*S**ACPAGGTSSPGIG*KVWPISS SDLAQVAGPAESDLHPQKYHSFSNPSEHQGV*L HL*PRRDEAAKCPEQKLEIYCAYAYGGWEESPK GCRASSVPL**PVLRPQLLGLPSSSAANEVLPQR KEGVNKAIGAS

Frame 2

RHLPHCAQEFSKPALRSE*PSSGQFDLEDPEVE TVLPPSSVFHPLQVLPREAAKAVHPARDPLCPA PAPPSGWNFPLTARLKLRIVGSLAGCRTEPRPQ YPIHALCLCQGFK*SSRLSLPRC*DYMYEPLHLE RSQKCEVLAEG*AAASQAKGAMAASCVLLHTGQ KMPLIGLGTWKSEPGQVKAAVKYALSVGYRHID CAAIYGNEPEIGEALKEDVGPGKAVPREELFVTS KLWNTKHHPEDVEPALRKTLADLQLEYLDLYLM HWPYAFERGDNPFPKNADGTICYDSTHYKETWK ALEALVAKGLVQALGLSNFNSRQIDDILSVASVR PAVLQVECHPYLAQNELIAHCQARGLEVTAYSP LGSSDRAWRDPDEPVLLEEPVVLALAEKYGRSP AQILLRWQVQRKVICIPKSITPSRILQNIKVFDF TFSPEEMKQLNALNKNWRYIVPMLTVDGKRVPR DAGHPLYPFNDPY*DHSFLASLPALQLMRSCHN GKRELIKPLEHP