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Sequences

Sequence NM_001017396

AACGCCAGCTGACTCGATTTCGTCTCGGTCTTCCCCAGAGCTGGACACGAGGCACCATGATCAGAGAGACACATTCCCTGCGGACGGCGCTCCCATCGGA TACTGGAGGGACGCGACACTAGAGGCAGGCAGTACTTCAGGGTGACCATAAGTCTCCCAAACAGCCACAACGGCTCGCTCTCCCAGCTAAGGCGACAGCG CTGCCATTTTGGATCCTGTTCAGCCACGGGAAGCCGGCGTTTGCACACATTTTCGGACGTCATATTAAAGCGCACCGGACGCCGAGACTTCTGGGAGATG AAGTCCTTGCGAAGGCCCCGCATTGATTGGGAGCGCAGTCGCCAGGACCCTGTGCGCAGGCGCGTTGGTTTCCCGACCTGAAGAGGCGCCGTCTTCCCGG GTCCCGAGCACTCTGTGCCGGAGGAATCAGTGACATTCGAAGACGTTGCCGTGGTTTTCACAGATGAAGAGTGGAGTCGTCTGGTCCCCATACAGAGGGA CCTCTACAAGGAGGTGATGCTGGAGAACTATAACAGCATTGTGTCATTGGGCCTTCCAGTTCCTCAACCTGATGTGATTTTCCAATTGAAGAGAGGGGAC AAGCCGTGGATGGTAGATCTTCATGGGTCTGAGGAGAGAGAATGGCCAGAGAGTGTCTCTCTAGACTGGGAAACTAAGCCTGAGATTCACGATGCTTCAG ACAAAAAATCAGAAGGATCATTGAGGGAATGCCTTGGAAGGCAAAGTCCTCTGTGTCCTAAATTTGAAGTTCATACACCCAATGGCAGGATGGGAACAGA AAAGCAAAGCCCTTCAGGGGAGACTCGTAAGAAATCCCTCTCCCGGGACAAAGGCTTGCGGCGGCGACGGTCAGCCCTGTCCAGGGAAATTCTCACTAAA GAGAGACACCAGGAATGCAGTGACTGTGGGAAGACCTTTTTTGACCACTCATCCCTCACCCGCCATCAGAGGACTCACACTGGGGAGAAGCCCTACGACT GCCGCGAGTGTGGGAAAGCCTTCAGCCACAGGAGCAGCCTCAGCAGACATCTGATGTCACACACTGGGGAGAGCCCCTACGAGTGCAGTGTGTGCTCAAA AGCCTTCTTTGACCGTTCGTCCCTAACTGTCCATCAGCGAATTCACACTGGAGAGAAACCCTTTCAGTGCAACGAGTGTGGAAAAGCCTTTTTTGACCGT TCATCCCTTACTCGACACCAGAGAATTCACACTGGAGAAAGTCCTTATGAATGTCATCAGTGTGGGAAAGCCTTTAGCCAGAAAAGTATTCTTACTCGCC ATCAGCTAATCCACACTGGCAGGAAGCCTTATGAGTGTAACGAGTGCGGGAAAGCTTTCTATGGTGTCTCGTCTCTGAATAGACATCAGAAAGCTCATGC TGGGGACCCTCGCTATCAGTGTAACGAGTGTGGCAAAGCTTTCTTTGACCGCTCATCCCTTACACAGCATCAGAAGATCCACACTGGAGACAAGCCATAT GAATGCAGCGAATGCGGGAAAGCCTTTAGCCAGCGGTGCCGGCTCACGCGGCATCAGCGTGTCCACACGGGAGAGAAGCCCTTTGAATGCACTGTGTGTG GGAAAGTTTTCAGTTCAAAATCTTCTGTTATTCAACATCAACGGCGTTACGCCAAACAGGGAATAGACTGAGTTGGGCAAAAGCTTGGGTAGGACAAGAA CTTCCATACAAATTTGAGATAGATCTCGCCTGTCTTAAAGCTGCCATTTGCTCATTCTACCCACTCTGGCTGCCGTTGTCCTGTCCTGCTTCTGCGCCAG AGTGTTTAATAAGCACTCCCTTCCTGCTGTGTTATAGAACTGTAGGGGAGGCCATGGAAATGACTCTAAAGATACAAAATTTTGAAGAGGTTTGTCAGAC AATAGGATAGATGTTAGGAGGAGACACACCTCTTGTCTGAGAACCTAGGCCCCAGGTATCACTGCACTTTAAGAAACTGGAGGCTGCAACAGGAGGGAAA GGCAGAATGATATCACAGCAGTACTATTTTTTTTTTTCAGAACATTTGTTTTGATGGCTGTTAGTCCCCCTCTCTTGGAGCATTTTGGGGCACTAATCTT GTTCTCTTGTGTCCCAGCTTTTAGACCTCTTACTTCCACTGCTAGTAGCTAGCACTTACTGAGCACCTGCGATGAGGTAAAGACTCAGCTAAGGGTTTTA CAGGAAGTAGATTAATTGATCCTGTCACCAACCATATGAAGTAGGCACTGTTACCATCTGCATCTCCAGATGAGCACCCTGAGGTTTAGAGAGATTAAAT CACTTGCCCAAGGTGAACCAGCTGGTAAAAGGTGATGCCAGGTCTTGAAGACAGGTCCACCTTCAAAATCTATTCTCTTTACCATTATGCTCGATGGTTT GTTTGTTTTATAATTTATGTATATGTATTATCCTCTCAACTAGATTGTAAGCACTTGGAGGTGTGACTTACAGGTCTTTGTATCTCACACAGTGCCTTGC TAATAGGTGCTCAATAAATATTTATATGAGTGAATGACTTTTCTACTTACCAAATTCTCTCCATTCAGAACACCTCTTGGATTTGCATACTTTCTCTGAA TTCACAGCCCAGCCTATACTTTTATCATCCCCAGCTCCCACTCCTGGCAGCAGTAGCTTTCTAATTCCAGTTCTTCCTGCTTTATTGAAGAGGGATGTGC TAAGCTGTGGTACAGTAACAAGCCCGCAGAGAGCTATGTCTCCCTTAAAACCTGACGGGAACCATTCTGTCTTATTCTATCCTCATTATTTGGAACATCT GGCTTCCAAAGTCACCACAAGGGCGGAAGAGAAAGCTAGAGGAATTTGTAGGATATTTTCTTAGACCCAGGCTTACATCACTTCTGTCCACATCTAATAA GCCAGAACCCAGCACCTAGAAGCTAGGAAAGTAAACAAGCACCTCGAAAGTTGGTTAATACCAAGAACCTCTCTCACAGTTATATTTTGCATACTACTGT TAAGCCAGTCTTTGCAAATGACCCCTTCCTGCCCATCACTGCCTTCCTCAAGACCTAAAATAGCTCCCTATTTAGTGAAAAATTATCTGAATATTTAAGG TCTGCCTTAACGTGATCCCCATTGCTGAATTTTACCTCCTGACTCCAAAAACTCTTCTCTTCCCTGGGCCCAGTCCTATTTTTTTTTTTTTTTTTTTTTT TGTGAGACAGAGTCTCACTCTGTCACCCAGGTTGGAATGCAATGGCACAATCTCCGCTCACTGCAAGCTCCGCCTCCCGGGTTCACGCCATTCTCCTGCC TCAGCCTCCCGAATAGCTGGGACTACAGGTGCCCGCCAACACGCCCGGCTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTCACCGTGTTAGCCAGGAT GGTCTCAATCTCCTGACCTCGTGATCCGCCCGCCTCGGCCTCCCAAAGTGCTGGGATTACAGACGTAAGTCACCGTGCCCGGCCTATATTTTTATTTTAT TAAAGGTTAGGCACTAGATGACCTTGACTCTAGGCATCTGCATTCTCTTGATCTTTGCTTAGGGTGAAGCTCAGAAAAGGACTTGGTTCTGAAAGGCTGT CTGGAATTAGATTGTCCTACGTGATTCCCAGTGAATTGAGATGAATTTCCTCTGGAAGTTCAAGTCGGATTCGAGAAGCTGAATTACCAAACAGACCCTC CCCAACCTACTAACGACCCTATTACTCTGTTCACCCTAGAGCAGACGAACGGCAGGGAAGACTTGATCTGTGGACTTTTGAAAGCAACTTGAGGAAAAAC ACATAACAGAATCTATAGGTGTGGACAATATGTGATATTGCTAAATAATGTCAAGTAGGAAATAAAAGAGACTATTGACAAA

refseq_ZNF2.F2 (forward primer)
refseq_ZNF2.R2 (reverse primer)
Single exon skipping

Frame 0

NAS*LDFVSVFPRAGHEAP*SERHIPCGRRSHR ILEGRDTRGRQYFRVTISLPNSHNGSLSQLRRQR CHFGSCSATGSRRLHTFSDVILKRTGRRDFWEM KSLRRPRIDWERSRQDPVRRRVGFPT*RGAVFP GPEHSVPEESVTFEDVAVVFTDEEWSRLVPIQRD LYKEVMLENYNSIVSLGLPVPQPDVIFQLKRGD KPWMVDLHGSEEREWPESVSLDWETKPEIHDAS DKKSEGSLRECLGRQSPLCPKFEVHTPNGRMGTE KQSPSGETRKKSLSRDKGLRRRRSALSREILTK ERHQECSDCGKTFFDHSSLTRHQRTHTGEKPYD CRECGKAFSHRSSLSRHLMSHTGESPYECSVCSK AFFDRSSLTVHQRIHTGEKPFQCNECGKAFFDR SSLTRHQRIHTGESPYECHQCGKAFSQKSILTR HQLIHTGRKPYECNECGKAFYGVSSLNRHQKAHA GDPRYQCNECGKAFFDRSSLTQHQKIHTGDKPY ECSECGKAFSQRCRLTRHQRVHTGEKPFECTVC GKVFSSKSSVIQHQRRYAKQGID*VGQKLG*DKN FHTNLR*ISPVLKLPFAHSTHSGCRCPVLLLRQ SV**ALPSCCVIEL*GRPWK*L*RYKILKRFVR Q*DRC*EETHLLSENLGPRYHCTLRNWRLQQEGK AE*YHSSTIFFFQNICFDGC*SPSLGAFWGTNL VLLCPSF*TSYFHC**LALTEHLR*GKDSAKGF TGSRLIDPVTNHMK*ALLPSASPDEHPEV*RD*I TCPR*TSW*KVMPGLEDRSTFKIYSLYHYARWF VCFIIYVYVLSSQLDCKHLEV*LTGLCISHSAL LIGAQ*IFI*VNDFSTYQILSIQNTSWICILSLN SQPSLYFYHPQLPLLAAVAF*FQFFLLY*RGMC *AVVQ*QARRELCLP*NLTGTILSYSILIIWNI WLPKSPQGRKRKLEEFVGYFLRPRLTSLLSTSNK PEPST*KLGK*TSTSKVG*YQEPLSQLYFAYYC *ASLCK*PLPAHHCLPQDLK*LPI**KII*IFK VCLNVIPIAEFYLLTPKTLLFPGPSPIFFFFFFF VRQSLTLSPRLECNGTISAHCKLRLPGSRHSPA SASRIAGTTGARQHARLIFCIFSRDGVSPC*PG WSQSPDLVIRPPRPPKVLGLQT*VTVPGLYFYFI KG*ALDDLDSRHLHSLDLCLG*SSEKDLVLKGC LELDCPT*FPVN*DEFPLEVQVGFEKLNYQTDP PQPTNDPITLFTLEQTNGREDLICGLLKAT*GKT HNRIYRCGQYVILLNNVK*EIKETIDK

Frame 1

TPADSISSRSSPELDTRHHDQRDTFPADGAPIG YWRDATLEAGSTSG*P*VSQTATTARSPS*GDS AAILDPVQPREAGVCTHFRTSY*SAPDAETSGR* SPCEGPALIGSAVARTLCAGALVSRPEEAPSSR VPSTLCRRNQ*HSKTLPWFSQMKSGVVWSPYRG TSTRR*CWRTITALCHWAFQFLNLM*FSN*REGT SRGW*IFMGLRRENGQRVSL*TGKLSLRFTMLQ TKNQKDH*GNALEGKVLCVLNLKFIHPMAGWEQ KSKALQGRLVRNPSPGTKACGGDGQPCPGKFSLK RDTRNAVTVGRPFLTTHPSPAIRGLTLGRSPTT AASVGKPSATGAASADI*CHTLGRAPTSAVCAQ KPSLTVRP*LSISEFTLERNPFSATSVEKPFLTV HPLLDTREFTLEKVLMNVISVGKPLARKVFLLA IS*STLAGSLMSVTSAGKLSMVSRL*IDIRKLM LGTLAISVTSVAKLSLTAHPLHSIRRSTLETSHM NAANAGKPLASGAGSRGISVSTRERSPLNALCV GKFSVQNLLLFNINGVTPNRE*TELGKSLGRTR TSIQI*DRSRLS*SCHLLILPTLAAVVLSCFCAR VFNKHSLPAVL*NCRGGHGNDSKDTKF*RGLSD NRIDVRRRHTSCLRT*APGITAL*ETGGCNRRE RQNDITAVLFFFFRTFVLMAVSPPLLEHFGALIL FSCVPAFRPLTSTASS*HLLSTCDEVKTQLRVL QEVD*LILSPTI*SRHCYHLHLQMSTLRFREIK SLAQGEPAGKR*CQVLKTGPPSKSILFTIMLDGL FVL*FMYMYYPLN*IVSTWRCDLQVFVSHTVPC **VLNKYLYE*MTFLLTKFSPFRTPLGFAYFL* IHSPAYTFIIPSSHSWQQ*LSNSSSSCFIEEGCA KLWYSNKPAESYVSLKT*REPFCLILSSLFGTS GFQSHHKGGRES*RNL*DIFLDPGLHHFCPHLI SQNPAPRS*ESKQAPRKLVNTKNLSHSYILHTTV KPVFANDPFLPITAFLKT*NSSLFSEKLSEYLR SALT*SPLLNFTS*LQKLFSSLGPVLFFFFFFF L*DRVSLCHPGWNAMAQSPLTASSASRVHAILLP QPPE*LGLQVPANTPG*FFVFLVETGFHRVSQD GLNLLTS*SARLGLPKCWDYRRKSPCPAYIFIL LKVRH*MTLTLGICILLIFA*GEAQKRTWF*KAV WN*IVLRDSQ*IEMNFLWKFKSDSRS*ITKQTL PNLLTTLLLCSP*SRRTAGKT*SVDF*KQLEEK HITESIGVDNM*YC*IMSSRK*KRLLT

Frame 2

RQLTRFRLGLPQSWTRGTMIRETHSLRTALPSD TGGTRH*RQAVLQGDHKSPKQPQRLALPAKATA LPFWILFSHGKPAFAHIFGRHIKAHRTPRLLGD EVLAKAPH*LGAQSPGPCAQARWFPDLKRRRLPG SRALCAGGISDIRRRCRGFHR*RVESSGPHTEG PLQGGDAGEL*QHCVIGPSSSST*CDFPIEERG QAVDGRSSWV*GERMARECLSRLGN*A*DSRCFR QKIRRIIEGMPWKAKSSVS*I*SSYTQWQDGNR KAKPFRGDS*EIPLPGQRLAAATVSPVQGNSH* RETPGMQ*LWEDLF*PLIPHPPSEDSHWGEALRL PRVWESLQPQEQPQQTSDVTHWGEPLRVQCVLK SLL*PFVPNCPSANSHWRETLSVQRVWKSLF*P FIPYSTPENSHWRKSL*MSSVWESL*PEKYSYSP SANPHWQEAL*V*RVRESFLWCLVSE*TSESSC WGPSLSV*RVWQSFL*PLIPYTASEDPHWRQAI *MQRMRESL*PAVPAHAASACPHGREAL*MHCVW ESFQFKIFCYSTSTALRQTGNRLSWAKAWVGQE LPYKFEIDLACLKAAICSFYPLWLPLSCPASAP ECLISTPFLLCYRTVGEAMEMTLKIQNFEEVCQT IG*MLGGDTPLV*EPRPQVSLHFKKLEAATGGK GRMISQQYYFFFSEHLF*WLLVPLSWSILGH*S CSLVSQLLDLLLPLLVASTY*APAMR*RLS*GFY RK*IN*SCHQPYEVGTVTICISR*AP*GLERLN HLPKVNQLVKGDARS*RQVHLQNLFSLPLCSMV CLFYNLCICIILSTRL*ALGGVTYRSLYLTQCLA NRCSINIYMSE*LFYLPNSLHSEHLLDLHTFSE FTAQPILLSSPAPTPGSSSFLIPVLPALLKRDV LSCGTVTSPQRAMSPLKPDGNHSVLFYPHYLEHL ASKVTTRAEEKARGICRIFS*TQAYITSVHI** ARTQHLEARKVNKHLESWLIPRTSLTVIFCILL LSQSLQMTPSCPSLPSSRPKIAPYLVKNYLNI*G LP*RDPHC*ILPPDSKNSSLPWAQSYFFFFFFF CETESHSVTQVGMQWHNLRSLQAPPPGFTPFSC LSLPNSWDYRCPPTRPANFLYF**RRGFTVLARM VSIS*PRDPPASASQSAGITDVSHRARPIFLFY *RLGTR*P*L*ASAFS*SLLRVKLRKGLGSERL SGIRLSYVIPSELR*ISSGSSSRIREAELPNRPS PTY*RPYYSVHPRADERQGRLDLWTFESNLRKN T*QNL*VWTICDIAK*CQVGNKRDY*Q

Sequence NM_021088

AACGCCAGCTGACTCGATTTCGTCTCGGTCTTCCCCAGAGCTGGACACGAGGCACCATGATCAGAGAGACACATTCCCTGCGGACGGCGCTCCCATCGGA TACTGGAGGGACGCGACACTAGAGGCAGGCAGTACTTCAGGGTGACCATAAGTCTCCCAAACAGCCACAACGGCTCGCTCTCCCAGCTAAGGCGACAGCG CTGCCATTTTGGATCCTGTTCAGCCACGGGAAGCCGGCGTTTGCACACATTTTCGGACGTCATATTAAAGCGCACCGGACGCCGAGACTTCTGGGAGATG AAGTCCTTGCGAAGGCCCCGCATTGATTGGGAGCGCAGTCGCCAGGACCCTGTGCGCAGGCGCGTTGGTTTCCCGACCTGAAGAGGCGCCGTCTTCCCGG GTCCCGAGCACTCTGTGCCGGAGGACTCTGCCCTTGTCCACAAGGAGAGCACACAGGAGAGAATGGCTGCTGTGTCTCCGACCACCAGATGCCAGGAATC AGTGACATTCGAAGACGTTGCCGTGGTTTTCACAGATGAAGAGTGGAGTCGTCTGGTCCCCATACAGAGGGACCTCTACAAGGAGGTGATGCTGGAGAAC TATAACAGCATTGTGTCATTGGGCCTTCCAGTTCCTCAACCTGATGTGATTTTCCAATTGAAGAGAGGGGACAAGCCGTGGATGGTAGATCTTCATGGGT CTGAGGAGAGAGAATGGCCAGAGAGTGTCTCTCTAGACTGGGAAACTAAGCCTGAGATTCACGATGCTTCAGACAAAAAATCAGAAGGATCATTGAGGGA ATGCCTTGGAAGGCAAAGTCCTCTGTGTCCTAAATTTGAAGTTCATACACCCAATGGCAGGATGGGAACAGAAAAGCAAAGCCCTTCAGGGGAGACTCGT AAGAAATCCCTCTCCCGGGACAAAGGCTTGCGGCGGCGACGGTCAGCCCTGTCCAGGGAAATTCTCACTAAAGAGAGACACCAGGAATGCAGTGACTGTG GGAAGACCTTTTTTGACCACTCATCCCTCACCCGCCATCAGAGGACTCACACTGGGGAGAAGCCCTACGACTGCCGCGAGTGTGGGAAAGCCTTCAGCCA CAGGAGCAGCCTCAGCAGACATCTGATGTCACACACTGGGGAGAGCCCCTACGAGTGCAGTGTGTGCTCAAAAGCCTTCTTTGACCGTTCGTCCCTAACT GTCCATCAGCGAATTCACACTGGAGAGAAACCCTTTCAGTGCAACGAGTGTGGAAAAGCCTTTTTTGACCGTTCATCCCTTACTCGACACCAGAGAATTC ACACTGGAGAAAGTCCTTATGAATGTCATCAGTGTGGGAAAGCCTTTAGCCAGAAAAGTATTCTTACTCGCCATCAGCTAATCCACACTGGCAGGAAGCC TTATGAGTGTAACGAGTGCGGGAAAGCTTTCTATGGTGTCTCGTCTCTGAATAGACATCAGAAAGCTCATGCTGGGGACCCTCGCTATCAGTGTAACGAG TGTGGCAAAGCTTTCTTTGACCGCTCATCCCTTACACAGCATCAGAAGATCCACACTGGAGACAAGCCATATGAATGCAGCGAATGCGGGAAAGCCTTTA GCCAGCGGTGCCGGCTCACGCGGCATCAGCGTGTCCACACGGGAGAGAAGCCCTTTGAATGCACTGTGTGTGGGAAAGTTTTCAGTTCAAAATCTTCTGT TATTCAACATCAACGGCGTTACGCCAAACAGGGAATAGACTGAGTTGGGCAAAAGCTTGGGTAGGACAAGAACTTCCATACAAATTTGAGATAGATCTCG CCTGTCTTAAAGCTGCCATTTGCTCATTCTACCCACTCTGGCTGCCGTTGTCCTGTCCTGCTTCTGCGCCAGAGTGTTTAATAAGCACTCCCTTCCTGCT GTGTTATAGAACTGTAGGGGAGGCCATGGAAATGACTCTAAAGATACAAAATTTTGAAGAGGTTTGTCAGACAATAGGATAGATGTTAGGAGGAGACACA CCTCTTGTCTGAGAACCTAGGCCCCAGGTATCACTGCACTTTAAGAAACTGGAGGCTGCAACAGGAGGGAAAGGCAGAATGATATCACAGCAGTACTATT TTTTTTTTTCAGAACATTTGTTTTGATGGCTGTTAGTCCCCCTCTCTTGGAGCATTTTGGGGCACTAATCTTGTTCTCTTGTGTCCCAGCTTTTAGACCT CTTACTTCCACTGCTAGTAGCTAGCACTTACTGAGCACCTGCGATGAGGTAAAGACTCAGCTAAGGGTTTTACAGGAAGTAGATTAATTGATCCTGTCAC CAACCATATGAAGTAGGCACTGTTACCATCTGCATCTCCAGATGAGCACCCTGAGGTTTAGAGAGATTAAATCACTTGCCCAAGGTGAACCAGCTGGTAA AAGGTGATGCCAGGTCTTGAAGACAGGTCCACCTTCAAAATCTATTCTCTTTACCATTATGCTCGATGGTTTGTTTGTTTTATAATTTATGTATATGTAT TATCCTCTCAACTAGATTGTAAGCACTTGGAGGTGTGACTTACAGGTCTTTGTATCTCACACAGTGCCTTGCTAATAGGTGCTCAATAAATATTTATATG AGTGAATGACTTTTCTACTTACCAAATTCTCTCCATTCAGAACACCTCTTGGATTTGCATACTTTCTCTGAATTCACAGCCCAGCCTATACTTTTATCAT CCCCAGCTCCCACTCCTGGCAGCAGTAGCTTTCTAATTCCAGTTCTTCCTGCTTTATTGAAGAGGGATGTGCTAAGCTGTGGTACAGTAACAAGCCCGCA GAGAGCTATGTCTCCCTTAAAACCTGACGGGAACCATTCTGTCTTATTCTATCCTCATTATTTGGAACATCTGGCTTCCAAAGTCACCACAAGGGCGGAA GAGAAAGCTAGAGGAATTTGTAGGATATTTTCTTAGACCCAGGCTTACATCACTTCTGTCCACATCTAATAAGCCAGAACCCAGCACCTAGAAGCTAGGA AAGTAAACAAGCACCTCGAAAGTTGGTTAATACCAAGAACCTCTCTCACAGTTATATTTTGCATACTACTGTTAAGCCAGTCTTTGCAAATGACCCCTTC CTGCCCATCACTGCCTTCCTCAAGACCTAAAATAGCTCCCTATTTAGTGAAAAATTATCTGAATATTTAAGGTCTGCCTTAACGTGATCCCCATTGCTGA ATTTTACCTCCTGACTCCAAAAACTCTTCTCTTCCCTGGGCCCAGTCCTATTTTTTTTTTTTTTTTTTTTTTTGTGAGACAGAGTCTCACTCTGTCACCC AGGTTGGAATGCAATGGCACAATCTCCGCTCACTGCAAGCTCCGCCTCCCGGGTTCACGCCATTCTCCTGCCTCAGCCTCCCGAATAGCTGGGACTACAG GTGCCCGCCAACACGCCCGGCTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTCACCGTGTTAGCCAGGATGGTCTCAATCTCCTGACCTCGTGATCCG CCCGCCTCGGCCTCCCAAAGTGCTGGGATTACAGACGTAAGTCACCGTGCCCGGCCTATATTTTTATTTTATTAAAGGTTAGGCACTAGATGACCTTGAC TCTAGGCATCTGCATTCTCTTGATCTTTGCTTAGGGTGAAGCTCAGAAAAGGACTTGGTTCTGAAAGGCTGTCTGGAATTAGATTGTCCTACGTGATTCC CAGTGAATTGAGATGAATTTCCTCTGGAAGTTCAAGTCGGATTCGAGAAGCTGAATTACCAAACAGACCCTCCCCAACCTACTAACGACCCTATTACTCT GTTCACCCTAGAGCAGACGAACGGCAGGGAAGACTTGATCTGTGGACTTTTGAAAGCAACTTGAGGAAAAACACATAACAGAATCTATAGGTGTGGACAA TATGTGATATTGCTAAATAATGTCAAGTAGGAAATAAAAGAGACTATTGACAAA

refseq_ZNF2.F2 (forward primer)
refseq_ZNF2.R2 (reverse primer)
Single exon skipping

Frame 0

NAS*LDFVSVFPRAGHEAP*SERHIPCGRRSHR ILEGRDTRGRQYFRVTISLPNSHNGSLSQLRRQR CHFGSCSATGSRRLHTFSDVILKRTGRRDFWEM KSLRRPRIDWERSRQDPVRRRVGFPT*RGAVFP GPEHSVPEDSALVHKESTQERMAAVSPTTRCQES VTFEDVAVVFTDEEWSRLVPIQRDLYKEVMLEN YNSIVSLGLPVPQPDVIFQLKRGDKPWMVDLHG SEEREWPESVSLDWETKPEIHDASDKKSEGSLRE CLGRQSPLCPKFEVHTPNGRMGTEKQSPSGETR KKSLSRDKGLRRRRSALSREILTKERHQECSDC GKTFFDHSSLTRHQRTHTGEKPYDCRECGKAFSH RSSLSRHLMSHTGESPYECSVCSKAFFDRSSLT VHQRIHTGEKPFQCNECGKAFFDRSSLTRHQRI HTGESPYECHQCGKAFSQKSILTRHQLIHTGRKP YECNECGKAFYGVSSLNRHQKAHAGDPRYQCNE CGKAFFDRSSLTQHQKIHTGDKPYECSECGKAF SQRCRLTRHQRVHTGEKPFECTVCGKVFSSKSSV IQHQRRYAKQGID*VGQKLG*DKNFHTNLR*IS PVLKLPFAHSTHSGCRCPVLLLRQSV**ALPSC CVIEL*GRPWK*L*RYKILKRFVRQ*DRC*EETH LLSENLGPRYHCTLRNWRLQQEGKAE*YHSSTI FFFQNICFDGC*SPSLGAFWGTNLVLLCPSF*T SYFHC**LALTEHLR*GKDSAKGFTGSRLIDPVT NHMK*ALLPSASPDEHPEV*RD*ITCPR*TSW* KVMPGLEDRSTFKIYSLYHYARWFVCFIIYVYV LSSQLDCKHLEV*LTGLCISHSALLIGAQ*IFI* VNDFSTYQILSIQNTSWICILSLNSQPSLYFYH PQLPLLAAVAF*FQFFLLY*RGMC*AVVQ*QAR RELCLP*NLTGTILSYSILIIWNIWLPKSPQGRK RKLEEFVGYFLRPRLTSLLSTSNKPEPST*KLG K*TSTSKVG*YQEPLSQLYFAYYC*ASLCK*PL PAHHCLPQDLK*LPI**KII*IFKVCLNVIPIAE FYLLTPKTLLFPGPSPIFFFFFFFVRQSLTLSP RLECNGTISAHCKLRLPGSRHSPASASRIAGTT GARQHARLIFCIFSRDGVSPC*PGWSQSPDLVIR PPRPPKVLGLQT*VTVPGLYFYFIKG*ALDDLD SRHLHSLDLCLG*SSEKDLVLKGCLELDCPT*F PVN*DEFPLEVQVGFEKLNYQTDPPQPTNDPITL FTLEQTNGREDLICGLLKAT*GKTHNRIYRCGQ YVILLNNVK*EIKETIDK

Frame 1

TPADSISSRSSPELDTRHHDQRDTFPADGAPIG YWRDATLEAGSTSG*P*VSQTATTARSPS*GDS AAILDPVQPREAGVCTHFRTSY*SAPDAETSGR* SPCEGPALIGSAVARTLCAGALVSRPEEAPSSR VPSTLCRRTLPLSTRRAHRREWLLCLRPPDARN Q*HSKTLPWFSQMKSGVVWSPYRGTSTRR*CWRT ITALCHWAFQFLNLM*FSN*REGTSRGW*IFMG LRRENGQRVSL*TGKLSLRFTMLQTKNQKDH*G NALEGKVLCVLNLKFIHPMAGWEQKSKALQGRLV RNPSPGTKACGGDGQPCPGKFSLKRDTRNAVTV GRPFLTTHPSPAIRGLTLGRSPTTAASVGKPSA TGAASADI*CHTLGRAPTSAVCAQKPSLTVRP*L SISEFTLERNPFSATSVEKPFLTVHPLLDTREF TLEKVLMNVISVGKPLARKVFLLAIS*STLAGS LMSVTSAGKLSMVSRL*IDIRKLMLGTLAISVTS VAKLSLTAHPLHSIRRSTLETSHMNAANAGKPL ASGAGSRGISVSTRERSPLNALCVGKFSVQNLL LFNINGVTPNRE*TELGKSLGRTRTSIQI*DRSR LS*SCHLLILPTLAAVVLSCFCARVFNKHSLPA VL*NCRGGHGNDSKDTKF*RGLSDNRIDVRRRH TSCLRT*APGITAL*ETGGCNRRERQNDITAVLF FFFRTFVLMAVSPPLLEHFGALILFSCVPAFRP LTSTASS*HLLSTCDEVKTQLRVLQEVD*LILS PTI*SRHCYHLHLQMSTLRFREIKSLAQGEPAGK R*CQVLKTGPPSKSILFTIMLDGLFVL*FMYMY YPLN*IVSTWRCDLQVFVSHTVPC**VLNKYLY E*MTFLLTKFSPFRTPLGFAYFL*IHSPAYTFII PSSHSWQQ*LSNSSSSCFIEEGCAKLWYSNKPA ESYVSLKT*REPFCLILSSLFGTSGFQSHHKGG RES*RNL*DIFLDPGLHHFCPHLISQNPAPRS*E SKQAPRKLVNTKNLSHSYILHTTVKPVFANDPF LPITAFLKT*NSSLFSEKLSEYLRSALT*SPLL NFTS*LQKLFSSLGPVLFFFFFFFL*DRVSLCHP GWNAMAQSPLTASSASRVHAILLPQPPE*LGLQ VPANTPG*FFVFLVETGFHRVSQDGLNLLTS*S ARLGLPKCWDYRRKSPCPAYIFILLKVRH*MTLT LGICILLIFA*GEAQKRTWF*KAVWN*IVLRDS Q*IEMNFLWKFKSDSRS*ITKQTLPNLLTTLLL CSP*SRRTAGKT*SVDF*KQLEEKHITESIGVDN M*YC*IMSSRK*KRLLT

Frame 2

RQLTRFRLGLPQSWTRGTMIRETHSLRTALPSD TGGTRH*RQAVLQGDHKSPKQPQRLALPAKATA LPFWILFSHGKPAFAHIFGRHIKAHRTPRLLGD EVLAKAPH*LGAQSPGPCAQARWFPDLKRRRLPG SRALCAGGLCPCPQGEHTGENGCCVSDHQMPGI SDIRRRCRGFHR*RVESSGPHTEGPLQGGDAGE L*QHCVIGPSSSST*CDFPIEERGQAVDGRSSWV *GERMARECLSRLGN*A*DSRCFRQKIRRIIEG MPWKAKSSVS*I*SSYTQWQDGNRKAKPFRGDS *EIPLPGQRLAAATVSPVQGNSH*RETPGMQ*LW EDLF*PLIPHPPSEDSHWGEALRLPRVWESLQP QEQPQQTSDVTHWGEPLRVQCVLKSLL*PFVPN CPSANSHWRETLSVQRVWKSLF*PFIPYSTPENS HWRKSL*MSSVWESL*PEKYSYSPSANPHWQEA L*V*RVRESFLWCLVSE*TSESSCWGPSLSV*R VWQSFL*PLIPYTASEDPHWRQAI*MQRMRESL* PAVPAHAASACPHGREAL*MHCVWESFQFKIFC YSTSTALRQTGNRLSWAKAWVGQELPYKFEIDL ACLKAAICSFYPLWLPLSCPASAPECLISTPFLL CYRTVGEAMEMTLKIQNFEEVCQTIG*MLGGDT PLV*EPRPQVSLHFKKLEAATGGKGRMISQQYY FFFSEHLF*WLLVPLSWSILGH*SCSLVSQLLDL LLPLLVASTY*APAMR*RLS*GFYRK*IN*SCH QPYEVGTVTICISR*AP*GLERLNHLPKVNQLV KGDARS*RQVHLQNLFSLPLCSMVCLFYNLCICI ILSTRL*ALGGVTYRSLYLTQCLANRCSINIYM SE*LFYLPNSLHSEHLLDLHTFSEFTAQPILLS SPAPTPGSSSFLIPVLPALLKRDVLSCGTVTSPQ RAMSPLKPDGNHSVLFYPHYLEHLASKVTTRAE EKARGICRIFS*TQAYITSVHI**ARTQHLEAR KVNKHLESWLIPRTSLTVIFCILLLSQSLQMTPS CPSLPSSRPKIAPYLVKNYLNI*GLP*RDPHC* ILPPDSKNSSLPWAQSYFFFFFFFCETESHSVT QVGMQWHNLRSLQAPPPGFTPFSCLSLPNSWDYR CPPTRPANFLYF**RRGFTVLARMVSIS*PRDP PASASQSAGITDVSHRARPIFLFY*RLGTR*P* L*ASAFS*SLLRVKLRKGLGSERLSGIRLSYVIP SELR*ISSGSSSRIREAELPNRPSPTY*RPYYS VHPRADERQGRLDLWTFESNLRKNT*QNL*VWT ICDIAK*CQVGNKRDY*Q