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Sequences

Sequence NM_006175

AAACTAGAAGGTCAGCAAAGGAAAGTGGAAGTTGGATTCTGAAAGATCGAGGTGCCCACAGGAATTTTATGGTCGTCGGATTTTGAAGACTTGAACTAGA CTGGGGGTTCTCCTTGCATTTCTTGCCTGTTGCCTATCTTTGTCCTCTCTCTTCCGGCTTCGAGATGAATGTGCAGCCCTGTTCTAGGTGTGGGTATGGG GTTTATCCTGCCGAGAAGATCAGCTGTATAGATCAGATATGGCATAAAGCCTGTTTTCACTGTGAAGTTTGCAAGATGATGCTGTCTGTTAATAACTTTG TGAGTCACCAGAAAAAGCCGTACTGTCACGCCCATAACCCTAAGAACAACACTTTCACCAGTGTCTATCACACTCCATTAAATCTAAATGTGAGGACATT TCCAGAGGCCATCAGTGGGATCCATGACCAAGAAGATGGTGAACAGTGTAAATCAGTTTTTCACTGGGACATGAAATCCAAGGATAAGGAAGGTGCACCT AACAGGCAGCCACTGGCAAATGAGAGAGCCTATTGGACTGGATATGGGGAAGGGAATGCTTGGTGCCCAGGAGCTCTGCCAGACCCCGAAATTGTAAGGA TGGTTGAGGCTCGAAAGTCTCTTGGTGAGGAATATACAGAAGACTATGAGCAACCCAGGGGCAAGGGGAGCTTTCCAGCCATGATCACACCTGCTTATCA AAGGGCCAAGAAAGCCAACCAGCTGGCCAGCCAAGTGGAGTATAAGAGAGGGCATGATGAACGCATCTCCAGGTTCTCCACGGTGGTGGATACTCCTGAG CTGCTACGGAGCAAGGCTGGGGCACAGCTTCAAAGTGATGTGAGATACACAGAGGACTATGAACAACAAAGAGGGAAAGGCAGTTTCCCTGCGATGATCA CACCCGCCTATCAGATAGCCAAAAGAGCCAATGAGCTGGCAAGTGATGTGAGGTACCATCAACAATATCAAAAAGAAATGAGGGGAATGGCTGGTCCAGC CATTGGAGCTGAGGGCATCTTGACAAGGGAATGTGCAGACCAATATGGCCAGGGTTACCCGGAGGAGTATGAGGAGCACAGGGGAAAGGGCAGCTTCCCA GCTATGATCACTCCAGCATATCAGAACGCCAAGAAAGCTCACGAACTCGCTAGTGACATAAAATACAGGCAGGACTTCAATAAGATGAAAGGCGCTGCAC ATTATCACTCGCTTCCAGCTCAAGACAACTTGGTTCTCAAACAGGCTCAGAGCGTAAACAAACTCGTGAGTGAGAATAAATATAAAGAAAACTACCAGAA CCACATGAGAGGCCGCTATGAAGGAGTTGGTATGGACAGACGCACTCTGCATGCTATGAAAGTTGGCAGCCTGGCAAGCAACGTTGCCTACAAAGCTGAT TATAAACATGATATTGTCGACTACAACTACCCAGCCACTCTCACGCCTTCCTATCAAACAGCTATGAAACTGGTGCCCTTGAAAGATGCCAATTATAGGC AGAGCATCGACAAGTTGAAGTACAGCTCGGTGACTGACACCCCACAGATTGTTCAAGCCAAAATCAATGCCCAGCAGCTGAGTCATGTGAATTACCGTGC TGACTATGAGAAAAATAAGTTGAATTACACATTGCCCCAGGATGTTCCTCAGCTGGTGAAGGCCAAAACCAATGCCAAACTCTTCAGTGAGGTTAAGTAT AAAGAAGGCTGGGAGAAGACAAAGGGGAAAGGATTTGAGATGAAGCTGGATGCCATGTCTCTGCTGGCCGCCAAAGCCTCTGGGGAGCTTGCTAGCAATA TTAAATATAAAGAAGAATATGAAAAGACAAAAGGCAAAGCCATGGGAACAGCCGACTCTAGGCTTCTGCACTCCCTGCAGATTGCTAAGATGAGCAGTGA GGTTGAATATAAGAAAGGCTTTGAAGAGAGTAAGACCCGGTTTCACCTGCCCATGGATATGGTAAACATCAGGCATGCTAAGAAGGCCCAAACTCTCGCC AGTGACCTGGACTACAGGAAGAAACTGCATGAATACACTGTGCTGCCTGAAGATATGAAGACTCAGTGGGCCAAGAAGGCCTATGGGCTCCAGAGCGAGC TGCAGTACAAGGCTGACCTGGCATGGATGAAGGGAGTTGGGTGGCTGACAGAGGGGAGTCTCAACTTGGAACAAGCCAAGAAGGCTGGACAGCTGGTCAG CGAGAAAAACTACCGGCAGAGGGTCGATGAGCTGAAGTTCACCAGCGTGACCGACAGCTCCCAGATGGAGCACGCCAAGAAGAGCCAGGAGCTACAGAGC GGGGTGGCCTACAAGGCAGGAAATGAGCAGTCTGTCCATCAGTATACCATCAGCAAAGACGAGCCTCTCTTCCTGCAGGCCCGAGCCAATGCTGCAAATC TCAGCGAGAAACTGTATAAGAGCAGCTGGGAAAACCAGAAAGCAAAAGGGTTTGAGCTGCGTCTTGATTCCTTGACCTTCCTGGCAGCCAAAGCCAAGAG GGACCTGGCTAGCGAGGTGAAGTACAAGGAGGATTATGAGAGATCCAGAGGGAAGCTCATTGGGGCAAAAGATGTACAGGGAGATTCGCAAATGAGCCAC TCACTGCAAATGTCCAAGCTGCAGAGTGAGCTGGAGTACAAGAAGGGATTCGAGGACACCAAATCCCAATGCCACGTCTCCCTGGACATGGTCCACCTCG TGCATGCCCGCAAAGCTCAGCATTTAGCCACAGACGTAGGCTACAAGACAGCGGAACATCACTTTACGGCTTTGCCCACAGACATGAAGGTGGAATGGGC CAAGAAGGCTTATGGCTTACAGAGTGATAACCAATACAGGGCAGATGTGAAGTGGATGAAAGGCATGGGCTGGGTCGCCACCGGGTCATTAAATGTGGAG CAGGCGAAGAAGGCAGGAGAACTCATTAGCGAGAAGAAGTACCGTCAGCATCCAGATGCTTTGAAGTTTACCAGTATTAAAGACACTCCGGAGATGGTCC AGGCCAGAATTAGTTATACCCAAGCAGTGGATAGATTATACAGGGAACAAGGAGAAAACATAAAGCATCATTACACACCGACTGCTGACCTCCCTGAAGT CCTGCTGGCCAAGCTGAATGCCATGAATATCAGTGAGACGCGTTATAAGGAATCCTGGAGCAAACTTCGAGATGGTGGCTATAAACTGAGGTTGGATGCC CTTCCATTCCAAGCAGCAAAGGCTTCTGGTGAAATCATAAGTGATTACAAATACAAAGAGGCATTTGAGAAAATGAAAGGACAGATGCTTGGTTCCCGGA GCTTGGAAGATGATATCAGCCTTGCACATTCAGTGTATGCGACCTCACTGCAGAGTGATGTGAATTACAAGAAAGGCTTTGAACACTCAAAGGCGCAGTT CCATCTGCCGTTGGACATGGCCGCCCTGGTGCATGCCAAGAAGGCTCAGACCCTGGCCAGCAATCAGGACTACAAACATCCACTGCCCCAGTACACTTCC TTGGCAGAAGACCTGAGGCTGAGCTGTGCCAAGAAAGCTCACAAATTGCAGAGTGAGAATTTGTACCGGTCAGACCTGAACTTTATGCGAGGTGTTGCAT GTGTCATTCCAGGCACGTTAGAGATTGAAGGGAGGAAGAAAGCATCGGAACTCATCAGTGAGAGTAAATATCGGCAGCATCCCCACTCATTCAAGTACAC AGCTGTGACAGACACTCCCAACCTCCTTCATGCGAAATTCAGCAACCAGATAACGAATGAGCGCCTCTATAAAGCAGCTGGAGAGGATGCAAGACACGAG TATACAATGACCCTGGGTCTGCCCGAGTTCATCCGAGCAAAAACGAATGCAGCCAACCTGAGTGACGCAAGATACAAAGAGTCCTGGCGTAATCTTCGTG CTCAAGGCTACAAGCTGACAATAGAAGCGCTCCCCTTCCAGGCTGCCCGGGCCTCTGGAGATATAGCCAGTGATTTTCTCTACAGACATGACTTTGTGAA GGAGCGAGGGAAACTCATAGGGCCCCAGAGTGTAAGAGACGACCCCCGGATCCAGCACTGCCGGCGCATGGGCCAGCTGCAGAGCGAGCTTCAGTACAGG AGGGGGGCGACCAGCAGCCAAGCCCAGTTCCATCTGCCCATGGACATGGTGCACCTGGTCCATGCCAAGAATGCCCAGGCTCTGGCCAGCGACCACGACT ACAGGACACAGTATCACAAGTTCACAGCACTGCCCGAGGACCTGAAGATGGCCTGGGCCAAGAAAGCCCATGCCCTGCAGAGTGAGTTGCGCTACAAGTC AGACCTGATCGGCATGAAGGGCATAGGATGGCTGGCGCTGAGATCCCCACAGATGGAGAGTGCAAAGAAGGCTGGAGAACTCATCAGCGAGACCAAGTAC CGTAAAAAACCAGACAGTATCAAGTTCACCACAGTGGTTGACTCCCCAGACCTGGTTCATGCCAAGAACAGCTATATGCACTGCAATGAGCGCATGTATA GATCTGGAGATGCAGAATCCCTGCACAGATACACCCTGATCCCCGACCATCCCGATTTCACCCGAGCTCGCCTCAATGCGCTGCATCTGAGTGACAAAGT CTACAGAAACTCCTGGGAGCAGACCCGGGCTGGCAGTTATGACTTCAGGCTGGATGCCATCCCCTTCCAGACTGCCCGGGCATCTAGGGAGATCGCCAGT GATTTCCGGTACAAAGAGGCTTTCCTGCGGGACCGAGGCCTGCAGATCGGGTACCGCAGTGTCGACGATGACCCAAGGATGAAGCATTTCCTCAACGTTG GCAGGCTCCAGAGTGACAATGAGTACAAGAAGGACTTTGCCAAGAGTCGGTCCCAGTTTCACAGCAGCACAGACCAGCCCGGCCTCCTTCAGGCCAAGAG GAGCCAGCAGCTGGCCAGTGATGTGCACTACAGGCAGCCCCTGCCCCAGCCCACCTGCGACCCGGAGCAGCTGGGCCTCAGGCATGCTCAGAAGGCCCAC CAGCTGCAGAGTGATGTCAAGTATAAATCAGACTTGAACCTGACCAGAGGTGTTGGCTGGACCCCTCCTGGCTCCTACAAAGTGGAAATGGCTCGGCGGG CTGCGGAACTGGCCAACGCAAGGGGCCTGGGTCTCCAGGGAGCTTACCGGGGGGCAGAAGCAGTGGAGGCTGGAGATCATCAGAGTGGGGAGGTGAACCC AGATGCCACTGAGATTCTGCACGTCAAAAAGAAGAAGGCCCTGCTGTTGTGAGCCATGTCCACCCTGATTCCTGAGAGGCCAGAGAGGAAGTTTGTTCAC CAGAGACAGGCTTCAGATGGCTTTGATTTCGGCAAGCTGGAATGGCCCACCAGCCCATCCCAGATCTTCCTTTATTAAAATAATAACTCTGAAAGCAGAT GGA

refseq_NRAP.F2 (forward primer)
refseq_NRAP.R2 (reverse primer)
Single exon skipping

Frame 0

KLEGQQRKVEVGF*KIEVPTGILWSSDFEDLN* TGGSPCISCLLPIFVLSLPASR*MCSPVLGVGMG FILPRRSAV*IRYGIKPVFTVKFAR*CCLLITL *VTRKSRTVTPITLRTTLSPVSITLH*I*M*GH FQRPSVGSMTKKMVNSVNQFFTGT*NPRIRKVHL TGSHWQMREPIGLDMGKGMLGAQELCQTPKL*G WLRLESLLVRNIQKTMSNPGARGAFQP*SHLLI KGPRKPTSWPAKWSIREGMMNASPGSPRWWILLS CYGARLGHSFKVM*DTQRTMNNKEGKAVSLR*S HPPIR*PKEPMSWQVM*GTINNIKKK*GEWLVQ PLELRAS*QGNVQTNMARVTRRSMRSTGERAASQ L*SLQHIRTPRKLTNSLVT*NTGRTSIR*KALH IITRFQLKTTWFSNRLRA*TNS*VRINIKKTTR TT*EAAMKELVWTDALCML*KLAAWQATLPTKLI INMILSTTTTQPLSRLPIKQL*NWCP*KMPIIG RASTS*STAR*LTPHRLFKPKSMPSS*VM*ITV LTMRKIS*ITHCPRMFLSW*RPKPMPNSSVRLSI KKAGRRQRGKDLR*SWMPCLCWPPKPLGSLLAI LNIKKNMKRQKAKPWEQPTLGFCTPCRLLR*AV RLNIRKALKRVRPGFTCPWIW*TSGMLRRPKLSP VTWTTGRNCMNTLCCLKI*RLSGPRRPMGSRAS CSTRLTWHG*RELGG*QRGVSTWNKPRRLDSWS ARKTTGRGSMS*SSPA*PTAPRWSTPRRARSYRA GWPTRQEMSSLSISIPSAKTSLSSCRPEPMLQI SARNCIRAAGKTRKQKGLSCVLIP*PSWQPKPR GTWLAR*STRRIMRDPEGSSLGQKMYREIRK*AT HCKCPSCRVSWSTRRDSRTPNPNATSPWTWSTS CMPAKLSI*PQT*ATRQRNITLRLCPQT*RWNG PRRLMAYRVITNTGQM*SG*KAWAGSPPGH*MWS RRRRQENSLARRSTVSIQML*SLPVLKTLRRWS RPELVIPKQWIDYTGNKEKT*SIITHRLLTSLK SCWPS*MP*ISVRRVIRNPGANFEMVAIN*GWMP FHSKQQRLLVKS*VITNTKRHLRK*KDRCLVPG AWKMISALHIQCMRPHCRVM*ITRKALNTQRRS SICRWTWPPWCMPRRLRPWPAIRTTNIHCPSTLP WQKT*G*AVPRKLTNCRVRICTGQT*TLCEVLH VSFQAR*RLKGGRKHRNSSVRVNIGSIPTHSST QL*QTLPTSFMRNSATR*RMSASIKQLERMQDTS IQ*PWVCPSSSEQKRMQPT*VTQDTKSPGVIFV LKATS*Q*KRSPSRLPGPLEI*PVIFSTDMTL* RSEGNS*GPRV*ETTPGSSTAGAWASCRASFSTG GGRPAAKPSSICPWTWCTWSMPRMPRLWPATTT TGHSITSSQHCPRT*RWPGPRKPMPCRVSCATS QT*SA*RA*DGWR*DPHRWRVQRRLENSSARPST VKNQTVSSSPQWLTPQTWFMPRTAICTAMSACI DLEMQNPCTDTP*SPTIPISPELASMRCI*VTK STETPGSRPGLAVMTSGWMPSPSRLPGHLGRSPV ISGTKRLSCGTEACRSGTAVSTMTQG*SISSTL AGSRVTMSTRRTLPRVGPSFTAAQTSPASFRPR GASSWPVMCTTGSPCPSPPATRSSWASGMLRRPT SCRVMSSINQT*T*PEVLAGPLLAPTKWKWLGG LRNWPTQGAWVSRELTGGQKQWRLEIIRVGR*T QMPLRFCTSKRRRPCCCEPCPP*FLRGQRGSLFT RDRLQMALISASWNGPPAHPRSSFIKIITLKAD G

Frame 1

N*KVSKGKWKLDSERSRCPQEFYGRRILKT*TR LGVLLAFLACCLSLSSLFRLRDECAALF*VWVW GLSCREDQLYRSDMA*SLFSL*SLQDDAVC**LC ESPEKAVLSRP*P*EQHFHQCLSHSIKSKCEDI SRGHQWDP*PRRW*TV*ISFSLGHEIQG*GRCT *QAATGK*ESLLDWIWGRECLVPRSSARPRNCKD G*GSKVSW*GIYRRL*ATQGQGELSSHDHTCLS KGQESQPAGQPSGV*ERA**THLQVLHGGGYS* AATEQGWGTASK*CEIHRGL*TTKRERQFPCDDH TRLSDSQKSQ*AGK*CEVPSTISKRNEGNGWSS HWS*GHLDKGMCRPIWPGLPGGV*GAQGKGQLP SYDHSSISERQESSRTR**HKIQAGLQ*DERRCT LSLASSSRQLGSQTGSERKQTRE*E*I*RKLPE PHERPL*RSWYGQTHSACYESWQPGKQRCLQS* L*T*YCRLQLPSHSHAFLSNSYETGALERCQL*A EHRQVEVQLGD*HPTDCSSQNQCPAAESCELPC *L*EK*VELHIAPGCSSAGEGQNQCQTLQ*G*V *RRLGEDKGERI*DEAGCHVSAGRQSLWGAC*QY *I*RRI*KDKRQSHGNSRL*ASALPADC*DEQ* G*I*ERL*RE*DPVSPAHGYGKHQAC*EGPNSR Q*PGLQEETA*IHCAA*RYEDSVGQEGLWAPERA AVQG*PGMDEGSWVADRGESQLGTSQEGWTAGQ REKLPAEGR*AEVHQRDRQLPDGARQEEPGATE RGGLQGRK*AVCPSVYHQQRRASLPAGPSQCCKS QRETV*EQLGKPESKRV*AAS*FLDLPGSQSQE GPG*RGEVQGGL*EIQREAHWGKRCTGRFANEP LTANVQAAE*AGVQEGIRGHQIPMPRLPGHGPPR ACPQSSAFSHRRRLQDSGTSLYGFAHRHEGGMG QEGLWLTE**PIQGRCEVDERHGLGRHRVIKCG AGEEGRRTH*REEVPSASRCFEVYQY*RHSGDGP GQN*LYPSSG*IIQGTRRKHKASLHTDC*PP*S PAGQAECHEYQ*DAL*GILEQTSRWWL*TEVGC PSIPSSKGFW*NHK*LQIQRGI*ENERTDAWFPE LGR*YQPCTFSVCDLTAE*CELQERL*TLKGAV PSAVGHGRPGACQEGSDPGQQSGLQTSTAPVHF LGRRPEAELCQESSQIAE*EFVPVRPELYARCCM CHSRHVRD*REEESIGTHQ*E*ISAASPLIQVH SCDRHSQPPSCEIQQPDNE*APL*SSWRGCKTR VYNDPGSARVHPSKNECSQPE*RKIQRVLA*SSC SRLQADNRSAPLPGCPGLWRYSQ*FSLQT*LCE GARETHRAPECKRRPPDPALPAHGPAAERASVQ EGGDQQPSPVPSAHGHGAPGPCQECPGSGQRPRL QDTVSQVHSTARGPEDGLGQESPCPAE*VALQV RPDRHEGHRMAGAEIPTDGECKEGWRTHQRDQV P*KTRQYQVHHSG*LPRPGSCQEQLYALQ*AHV* IWRCRIPAQIHPDPRPSRFHPSSPQCAASE*QS LQKLLGADPGWQL*LQAGCHPLPDCPGI*GDRQ *FPVQRGFPAGPRPADRVPQCRR*PKDEAFPQRW QAPE*Q*VQEGLCQESVPVSQQHRPARPPSGQE EPAAGQ*CALQAAPAPAHLRPGAAGPQACSEGP PAAE*CQV*IRLEPDQRCWLDPSWLLQSGNGSAG CGTGQRKGPGSPGSLPGGRSSGGWRSSEWGGEP RCH*DSARQKEEGPAVVSHVHPDS*EAREEVCS PETGFRWL*FRQAGMAHQPIPDLPLLK**L*KQM

Frame 2

TRRSAKESGSWILKDRGAHRNFMVVGF*RLELD WGFSLHFLPVAYLCPLSSGFEMNVQPCSRCGYG VYPAEKISCIDQIWHKACFHCEVCKMMLSVNNF VSHQKKPYCHAHNPKNNTFTSVYHTPLNLNVRTF PEAISGIHDQEDGEQCKSVFHWDMKSKDKEGAP NRQPLANERAYWTGYGEGNAWCPGALPDPEIVR MVEARKSLGEEYTEDYEQPRGKGSFPAMITPAYQ RAKKANQLASQVEYKRGHDERISRFSTVVDTPE LLRSKAGAQLQSDVRYTEDYEQQRGKGSFPAMI TPAYQIAKRANELASDVRYHQQYQKEMRGMAGPA IGAEGILTRECADQYGQGYPEEYEEHRGKGSFP AMITPAYQNAKKAHELASDIKYRQDFNKMKGAA HYHSLPAQDNLVLKQAQSVNKLVSENKYKENYQN HMRGRYEGVGMDRRTLHAMKVGSLASNVAYKAD YKHDIVDYNYPATLTPSYQTAMKLVPLKDANYR QSIDKLKYSSVTDTPQIVQAKINAQQLSHVNYRA DYEKNKLNYTLPQDVPQLVKAKTNAKLFSEVKY KEGWEKTKGKGFEMKLDAMSLLAAKASGELASN IKYKEEYEKTKGKAMGTADSRLLHSLQIAKMSSE VEYKKGFEESKTRFHLPMDMVNIRHAKKAQTLA SDLDYRKKLHEYTVLPEDMKTQWAKKAYGLQSE LQYKADLAWMKGVGWLTEGSLNLEQAKKAGQLVS EKNYRQRVDELKFTSVTDSSQMEHAKKSQELQS GVAYKAGNEQSVHQYTISKDEPLFLQARANAAN LSEKLYKSSWENQKAKGFELRLDSLTFLAAKAKR DLASEVKYKEDYERSRGKLIGAKDVQGDSQMSH SLQMSKLQSELEYKKGFEDTKSQCHVSLDMVHL VHARKAQHLATDVGYKTAEHHFTALPTDMKVEWA KKAYGLQSDNQYRADVKWMKGMGWVATGSLNVE QAKKAGELISEKKYRQHPDALKFTSIKDTPEMV QARISYTQAVDRLYREQGENIKHHYTPTADLPEV LLAKLNAMNISETRYKESWSKLRDGGYKLRLDA LPFQAAKASGEIISDYKYKEAFEKMKGQMLGSR SLEDDISLAHSVYATSLQSDVNYKKGFEHSKAQF HLPLDMAALVHAKKAQTLASNQDYKHPLPQYTS LAEDLRLSCAKKAHKLQSENLYRSDLNFMRGVA CVIPGTLEIEGRKKASELISESKYRQHPHSFKYT AVTDTPNLLHAKFSNQITNERLYKAAGEDARHE YTMTLGLPEFIRAKTNAANLSDARYKESWRNLR AQGYKLTIEALPFQAARASGDIASDFLYRHDFVK ERGKLIGPQSVRDDPRIQHCRRMGQLQSELQYR RGATSSQAQFHLPMDMVHLVHAKNAQALASDHD YRTQYHKFTALPEDLKMAWAKKAHALQSELRYKS DLIGMKGIGWLALRSPQMESAKKAGELISETKY RKKPDSIKFTTVVDSPDLVHAKNSYMHCNERMY RSGDAESLHRYTLIPDHPDFTRARLNALHLSDKV YRNSWEQTRAGSYDFRLDAIPFQTARASREIAS DFRYKEAFLRDRGLQIGYRSVDDDPRMKHFLNV GRLQSDNEYKKDFAKSRSQFHSSTDQPGLLQAKR SQQLASDVHYRQPLPQPTCDPEQLGLRHAQKAH QLQSDVKYKSDLNLTRGVGWTPPGSYKVEMARR AAELANARGLGLQGAYRGAEAVEAGDHQSGEVNP DATEILHVKKKKALLL*AMSTLIPERPERKFVH QRQASDGFDFGKLEWPTSPSQIFLY*NNNSESR W

Sequence NM_198060

AAACTAGAAGGTCAGCAAAGGAAAGTGGAAGTTGGATTCTGAAAGATCGAGGTGCCCACAGGAATTTTATGGTCGTCGGATTTTGAAGACTTGAACTAGA CTGGGGGTTCTCCTTGCATTTCTTGCCTGTTGCCTATCTTTGTCCTCTCTCTTCCGGCTTCGAGATGAATGTGCAGCCCTGTTCTAGGTGTGGGTATGGG GTTTATCCTGCCGAGAAGATCAGCTGTATAGATCAGATATGGCATAAAGCCTGTTTTCACTGTGAAGTTTGCAAGATGATGCTGTCTGTTAATAACTTTG TGAGTCACCAGAAAAAGCCGTACTGTCACGCCCATAACCCTAAGAACAACACTTTCACCAGTGTCTATCACACTCCATTAAATCTAAATGTGAGGACATT TCCAGAGGCCATCAGTGGGATCCATGACCAAGAAGATGGTGAACAGTGTAAATCAGTTTTTCACTGGGACATGAAATCCAAGGATAAGGAAGGTGCACCT AACAGGCAGCCACTGGCAAATGAGAGAGCCTATTGGACTGGATATGGGGAAGGGAATGCTTGGTGCCCAGGAGCTCTGCCAGACCCCGAAATTGTAAGGA TGGTTGAGGCTCGAAAGTCTCTTGGTGAGGAATATACAGAAGACTATGAGCAACCCAGGGGCAAGGGGAGCTTTCCAGCCATGATCACACCTGCTTATCA AAGGGCCAAGAAAGCCAACCAGCTGGCCAGCCAAGTGGAGTATAAGAGAGGGCATGATGAACGCATCTCCAGGTTCTCCACGGTGGTGGATACTCCTGAG CTGCTACGGAGCAAGGCTGGGGCACAGCTTCAAAGTGATGTGAGATACACAGAGGACTATGAACAACAAAGAGGGAAAGGCAGTTTCCCTGCGATGATCA CACCCGCCTATCAGATAGCCAAAAGAGCCAATGAGCTGGCAAGTGATGTGAGGTACCATCAACAATATCAAAAAGAAATGAGGGGAATGGCTGGTCCAGC CATTGGAGCTGAGGGCATCTTGACAAGGGAATGTGCAGACCAATATGGCCAGGGTTACCCGGAGGAGTATGAGGAGCACAGGGGAAAGGGCAGCTTCCCA GCTATGATCACTCCAGCATATCAGAACGCCAAGAAAGCTCACGAACTCGCTAGTGACATAAAATACAGGCAGGACTTCAATAAGATGAAAGGCGCTGCAC ATTATCACTCGCTTCCAGCTCAAGACAACTTGGTTCTCAAACAGGCTCAGAGCGTAAACAAACTCGTGAGTGAGGTGGAGTATAAGAAGGATCTGGAAAG TAGTAGAGGTCACAGTATCAACTACTGTGAAACACCTCAATTCAGGAACGTGAGCAAGATCTCAAAATTTACCAGTGATAATAAATATAAAGAAAACTAC CAGAACCACATGAGAGGCCGCTATGAAGGAGTTGGTATGGACAGACGCACTCTGCATGCTATGAAAGTTGGCAGCCTGGCAAGCAACGTTGCCTACAAAG CTGATTATAAACATGATATTGTCGACTACAACTACCCAGCCACTCTCACGCCTTCCTATCAAACAGCTATGAAACTGGTGCCCTTGAAAGATGCCAATTA TAGGCAGAGCATCGACAAGTTGAAGTACAGCTCGGTGACTGACACCCCACAGATTGTTCAAGCCAAAATCAATGCCCAGCAGCTGAGTCATGTGAATTAC CGTGCTGACTATGAGAAAAATAAGTTGAATTACACATTGCCCCAGGATGTTCCTCAGCTGGTGAAGGCCAAAACCAATGCCAAACTCTTCAGTGAGGTTA AGTATAAAGAAGGCTGGGAGAAGACAAAGGGGAAAGGATTTGAGATGAAGCTGGATGCCATGTCTCTGCTGGCCGCCAAAGCCTCTGGGGAGCTTGCTAG CAATATTAAATATAAAGAAGAATATGAAAAGACAAAAGGCAAAGCCATGGGAACAGCCGACTCTAGGCTTCTGCACTCCCTGCAGATTGCTAAGATGAGC AGTGAGGTTGAATATAAGAAAGGCTTTGAAGAGAGTAAGACCCGGTTTCACCTGCCCATGGATATGGTAAACATCAGGCATGCTAAGAAGGCCCAAACTC TCGCCAGTGACCTGGACTACAGGAAGAAACTGCATGAATACACTGTGCTGCCTGAAGATATGAAGACTCAGTGGGCCAAGAAGGCCTATGGGCTCCAGAG CGAGCTGCAGTACAAGGCTGACCTGGCATGGATGAAGGGAGTTGGGTGGCTGACAGAGGGGAGTCTCAACTTGGAACAAGCCAAGAAGGCTGGACAGCTG GTCAGCGAGAAAAACTACCGGCAGAGGGTCGATGAGCTGAAGTTCACCAGCGTGACCGACAGCTCCCAGATGGAGCACGCCAAGAAGAGCCAGGAGCTAC AGAGCGGGGTGGCCTACAAGGCAGGAAATGAGCAGTCTGTCCATCAGTATACCATCAGCAAAGACGAGCCTCTCTTCCTGCAGGCCCGAGCCAATGCTGC AAATCTCAGCGAGAAACTGTATAAGAGCAGCTGGGAAAACCAGAAAGCAAAAGGGTTTGAGCTGCGTCTTGATTCCTTGACCTTCCTGGCAGCCAAAGCC AAGAGGGACCTGGCTAGCGAGGTGAAGTACAAGGAGGATTATGAGAGATCCAGAGGGAAGCTCATTGGGGCAAAAGATGTACAGGGAGATTCGCAAATGA GCCACTCACTGCAAATGTCCAAGCTGCAGAGTGAGCTGGAGTACAAGAAGGGATTCGAGGACACCAAATCCCAATGCCACGTCTCCCTGGACATGGTCCA CCTCGTGCATGCCCGCAAAGCTCAGCATTTAGCCACAGACGTAGGCTACAAGACAGCGGAACATCACTTTACGGCTTTGCCCACAGACATGAAGGTGGAA TGGGCCAAGAAGGCTTATGGCTTACAGAGTGATAACCAATACAGGGCAGATGTGAAGTGGATGAAAGGCATGGGCTGGGTCGCCACCGGGTCATTAAATG TGGAGCAGGCGAAGAAGGCAGGAGAACTCATTAGCGAGAAGAAGTACCGTCAGCATCCAGATGCTTTGAAGTTTACCAGTATTAAAGACACTCCGGAGAT GGTCCAGGCCAGAATTAGTTATACCCAAGCAGTGGATAGATTATACAGGGAACAAGGAGAAAACATAAAGCATCATTACACACCGACTGCTGACCTCCCT GAAGTCCTGCTGGCCAAGCTGAATGCCATGAATATCAGTGAGACGCGTTATAAGGAATCCTGGAGCAAACTTCGAGATGGTGGCTATAAACTGAGGTTGG ATGCCCTTCCATTCCAAGCAGCAAAGGCTTCTGGTGAAATCATAAGTGATTACAAATACAAAGAGGCATTTGAGAAAATGAAAGGACAGATGCTTGGTTC CCGGAGCTTGGAAGATGATATCAGCCTTGCACATTCAGTGTATGCGACCTCACTGCAGAGTGATGTGAATTACAAGAAAGGCTTTGAACACTCAAAGGCG CAGTTCCATCTGCCGTTGGACATGGCCGCCCTGGTGCATGCCAAGAAGGCTCAGACCCTGGCCAGCAATCAGGACTACAAACATCCACTGCCCCAGTACA CTTCCTTGGCAGAAGACCTGAGGCTGAGCTGTGCCAAGAAAGCTCACAAATTGCAGAGTGAGAATTTGTACCGGTCAGACCTGAACTTTATGCGAGGTGT TGCATGTGTCATTCCAGGCACGTTAGAGATTGAAGGGAGGAAGAAAGCATCGGAACTCATCAGTGAGAGTAAATATCGGCAGCATCCCCACTCATTCAAG TACACAGCTGTGACAGACACTCCCAACCTCCTTCATGCGAAATTCAGCAACCAGATAACGAATGAGCGCCTCTATAAAGCAGCTGGAGAGGATGCAAGAC ACGAGTATACAATGACCCTGGGTCTGCCCGAGTTCATCCGAGCAAAAACGAATGCAGCCAACCTGAGTGACGCAAGATACAAAGAGTCCTGGCGTAATCT TCGTGCTCAAGGCTACAAGCTGACAATAGAAGCGCTCCCCTTCCAGGCTGCCCGGGCCTCTGGAGATATAGCCAGTGATTTTCTCTACAGACATGACTTT GTGAAGGAGCGAGGGAAACTCATAGGGCCCCAGAGTGTAAGAGACGACCCCCGGATCCAGCACTGCCGGCGCATGGGCCAGCTGCAGAGCGAGCTTCAGT ACAGGAGGGGGGCGACCAGCAGCCAAGCCCAGTTCCATCTGCCCATGGACATGGTGCACCTGGTCCATGCCAAGAATGCCCAGGCTCTGGCCAGCGACCA CGACTACAGGACACAGTATCACAAGTTCACAGCACTGCCCGAGGACCTGAAGATGGCCTGGGCCAAGAAAGCCCATGCCCTGCAGAGTGAGTTGCGCTAC AAGTCAGACCTGATCGGCATGAAGGGCATAGGATGGCTGGCGCTGAGATCCCCACAGATGGAGAGTGCAAAGAAGGCTGGAGAACTCATCAGCGAGACCA AGTACCGTAAAAAACCAGACAGTATCAAGTTCACCACAGTGGTTGACTCCCCAGACCTGGTTCATGCCAAGAACAGCTATATGCACTGCAATGAGCGCAT GTATAGATCTGGAGATGCAGAATCCCTGCACAGATACACCCTGATCCCCGACCATCCCGATTTCACCCGAGCTCGCCTCAATGCGCTGCATCTGAGTGAC AAAGTCTACAGAAACTCCTGGGAGCAGACCCGGGCTGGCAGTTATGACTTCAGGCTGGATGCCATCCCCTTCCAGACTGCCCGGGCATCTAGGGAGATCG CCAGTGATTTCCGGTACAAAGAGGCTTTCCTGCGGGACCGAGGCCTGCAGATCGGGTACCGCAGTGTCGACGATGACCCAAGGATGAAGCATTTCCTCAA CGTTGGCAGGCTCCAGAGTGACAATGAGTACAAGAAGGACTTTGCCAAGAGTCGGTCCCAGTTTCACAGCAGCACAGACCAGCCCGGCCTCCTTCAGGCC AAGAGGAGCCAGCAGCTGGCCAGTGATGTGCACTACAGGCAGCCCCTGCCCCAGCCCACCTGCGACCCGGAGCAGCTGGGCCTCAGGCATGCTCAGAAGG CCCACCAGCTGCAGAGTGATGTCAAGTATAAATCAGACTTGAACCTGACCAGAGGTGTTGGCTGGACCCCTCCTGGCTCCTACAAAGTGGAAATGGCTCG GCGGGCTGCGGAACTGGCCAACGCAAGGGGCCTGGGTCTCCAGGGAGCTTACCGGGGGGCAGAAGCAGTGGAGGCTGGAGATCATCAGAGTGGGGAGGTG AACCCAGATGCCACTGAGATTCTGCACGTCAAAAAGAAGAAGGCCCTGCTGTTGTGAGCCATGTCCACCCTGATTCCTGAGAGGCCAGAGAGGAAGTTTG TTCACCAGAGACAGGCTTCAGATGGCTTTGATTTCGGCAAGCTGGAATGGCCCACCAGCCCATCCCAGATCTTCCTTTATTAAAATAATAACTCTGAAAG CAGATGGA

refseq_NRAP.F2 (forward primer)
refseq_NRAP.R2 (reverse primer)
Single exon skipping

Frame 0

KLEGQQRKVEVGF*KIEVPTGILWSSDFEDLN* TGGSPCISCLLPIFVLSLPASR*MCSPVLGVGMG FILPRRSAV*IRYGIKPVFTVKFAR*CCLLITL *VTRKSRTVTPITLRTTLSPVSITLH*I*M*GH FQRPSVGSMTKKMVNSVNQFFTGT*NPRIRKVHL TGSHWQMREPIGLDMGKGMLGAQELCQTPKL*G WLRLESLLVRNIQKTMSNPGARGAFQP*SHLLI KGPRKPTSWPAKWSIREGMMNASPGSPRWWILLS CYGARLGHSFKVM*DTQRTMNNKEGKAVSLR*S HPPIR*PKEPMSWQVM*GTINNIKKK*GEWLVQ PLELRAS*QGNVQTNMARVTRRSMRSTGERAASQ L*SLQHIRTPRKLTNSLVT*NTGRTSIR*KALH IITRFQLKTTWFSNRLRA*TNS*VRWSIRRIWK VVEVTVSTTVKHLNSGT*ARSQNLPVIINIKKTT RTT*EAAMKELVWTDALCML*KLAAWQATLPTK LIINMILSTTTTQPLSRLPIKQL*NWCP*KMPI IGRASTS*STAR*LTPHRLFKPKSMPSS*VM*IT VLTMRKIS*ITHCPRMFLSW*RPKPMPNSSVRL SIKKAGRRQRGKDLR*SWMPCLCWPPKPLGSLL AILNIKKNMKRQKAKPWEQPTLGFCTPCRLLR*A VRLNIRKALKRVRPGFTCPWIW*TSGMLRRPKL SPVTWTTGRNCMNTLCCLKI*RLSGPRRPMGSR ASCSTRLTWHG*RELGG*QRGVSTWNKPRRLDSW SARKTTGRGSMS*SSPA*PTAPRWSTPRRARSY RAGWPTRQEMSSLSISIPSAKTSLSSCRPEPML QISARNCIRAAGKTRKQKGLSCVLIP*PSWQPKP RGTWLAR*STRRIMRDPEGSSLGQKMYREIRK* ATHCKCPSCRVSWSTRRDSRTPNPNATSPWTWS TSCMPAKLSI*PQT*ATRQRNITLRLCPQT*RWN GPRRLMAYRVITNTGQM*SG*KAWAGSPPGH*M WSRRRRQENSLARRSTVSIQML*SLPVLKTLRR WSRPELVIPKQWIDYTGNKEKT*SIITHRLLTSL KSCWPS*MP*ISVRRVIRNPGANFEMVAIN*GW MPFHSKQQRLLVKS*VITNTKRHLRK*KDRCLV PGAWKMISALHIQCMRPHCRVM*ITRKALNTQRR SSICRWTWPPWCMPRRLRPWPAIRTTNIHCPST LPWQKT*G*AVPRKLTNCRVRICTGQT*TLCEV LHVSFQAR*RLKGGRKHRNSSVRVNIGSIPTHSS TQL*QTLPTSFMRNSATR*RMSASIKQLERMQD TSIQ*PWVCPSSSEQKRMQPT*VTQDTKSPGVI FVLKATS*Q*KRSPSRLPGPLEI*PVIFSTDMTL *RSEGNS*GPRV*ETTPGSSTAGAWASCRASFS TGGGRPAAKPSSICPWTWCTWSMPRMPRLWPAT TTTGHSITSSQHCPRT*RWPGPRKPMPCRVSCAT SQT*SA*RA*DGWR*DPHRWRVQRRLENSSARP STVKNQTVSSSPQWLTPQTWFMPRTAICTAMSA CIDLEMQNPCTDTP*SPTIPISPELASMRCI*VT KSTETPGSRPGLAVMTSGWMPSPSRLPGHLGRS PVISGTKRLSCGTEACRSGTAVSTMTQG*SISS TLAGSRVTMSTRRTLPRVGPSFTAAQTSPASFRP RGASSWPVMCTTGSPCPSPPATRSSWASGMLRR PTSCRVMSSINQT*T*PEVLAGPLLAPTKWKWL GGLRNWPTQGAWVSRELTGGQKQWRLEIIRVGR* TQMPLRFCTSKRRRPCCCEPCPP*FLRGQRGSL FTRDRLQMALISASWNGPPAHPRSSFIKIITLK ADG

Frame 1

N*KVSKGKWKLDSERSRCPQEFYGRRILKT*TR LGVLLAFLACCLSLSSLFRLRDECAALF*VWVW GLSCREDQLYRSDMA*SLFSL*SLQDDAVC**LC ESPEKAVLSRP*P*EQHFHQCLSHSIKSKCEDI SRGHQWDP*PRRW*TV*ISFSLGHEIQG*GRCT *QAATGK*ESLLDWIWGRECLVPRSSARPRNCKD G*GSKVSW*GIYRRL*ATQGQGELSSHDHTCLS KGQESQPAGQPSGV*ERA**THLQVLHGGGYS* AATEQGWGTASK*CEIHRGL*TTKRERQFPCDDH TRLSDSQKSQ*AGK*CEVPSTISKRNEGNGWSS HWS*GHLDKGMCRPIWPGLPGGV*GAQGKGQLP SYDHSSISERQESSRTR**HKIQAGLQ*DERRCT LSLASSSRQLGSQTGSERKQTRE*GGV*EGSGK **RSQYQLL*NTSIQEREQDLKIYQ***I*RKL PEPHERPL*RSWYGQTHSACYESWQPGKQRCLQS *L*T*YCRLQLPSHSHAFLSNSYETGALERCQL *AEHRQVEVQLGD*HPTDCSSQNQCPAAESCEL PC*L*EK*VELHIAPGCSSAGEGQNQCQTLQ*G* V*RRLGEDKGERI*DEAGCHVSAGRQSLWGAC* QY*I*RRI*KDKRQSHGNSRL*ASALPADC*DE Q*G*I*ERL*RE*DPVSPAHGYGKHQAC*EGPNS RQ*PGLQEETA*IHCAA*RYEDSVGQEGLWAPE RAAVQG*PGMDEGSWVADRGESQLGTSQEGWTA GQREKLPAEGR*AEVHQRDRQLPDGARQEEPGAT ERGGLQGRK*AVCPSVYHQQRRASLPAGPSQCC KSQRETV*EQLGKPESKRV*AAS*FLDLPGSQS QEGPG*RGEVQGGL*EIQREAHWGKRCTGRFANE PLTANVQAAE*AGVQEGIRGHQIPMPRLPGHGP PRACPQSSAFSHRRRLQDSGTSLYGFAHRHEGG MGQEGLWLTE**PIQGRCEVDERHGLGRHRVIKC GAGEEGRRTH*REEVPSASRCFEVYQY*RHSGD GPGQN*LYPSSG*IIQGTRRKHKASLHTDC*PP *SPAGQAECHEYQ*DAL*GILEQTSRWWL*TEVG CPSIPSSKGFW*NHK*LQIQRGI*ENERTDAWF PELGR*YQPCTFSVCDLTAE*CELQERL*TLKG AVPSAVGHGRPGACQEGSDPGQQSGLQTSTAPVH FLGRRPEAELCQESSQIAE*EFVPVRPELYARC CMCHSRHVRD*REEESIGTHQ*E*ISAASPLIQ VHSCDRHSQPPSCEIQQPDNE*APL*SSWRGCKT RVYNDPGSARVHPSKNECSQPE*RKIQRVLA*S SCSRLQADNRSAPLPGCPGLWRYSQ*FSLQT*L CEGARETHRAPECKRRPPDPALPAHGPAAERASV QEGGDQQPSPVPSAHGHGAPGPCQECPGSGQRP RLQDTVSQVHSTARGPEDGLGQESPCPAE*VAL QVRPDRHEGHRMAGAEIPTDGECKEGWRTHQRDQ VP*KTRQYQVHHSG*LPRPGSCQEQLYALQ*AH V*IWRCRIPAQIHPDPRPSRFHPSSPQCAASE* QSLQKLLGADPGWQL*LQAGCHPLPDCPGI*GDR Q*FPVQRGFPAGPRPADRVPQCRR*PKDEAFPQ RWQAPE*Q*VQEGLCQESVPVSQQHRPARPPSG QEEPAAGQ*CALQAAPAPAHLRPGAAGPQACSEG PPAAE*CQV*IRLEPDQRCWLDPSWLLQSGNGS AGCGTGQRKGPGSPGSLPGGRSSGGWRSSEWGG EPRCH*DSARQKEEGPAVVSHVHPDS*EAREEVC SPETGFRWL*FRQAGMAHQPIPDLPLLK**L*K QM

Frame 2

TRRSAKESGSWILKDRGAHRNFMVVGF*RLELD WGFSLHFLPVAYLCPLSSGFEMNVQPCSRCGYG VYPAEKISCIDQIWHKACFHCEVCKMMLSVNNF VSHQKKPYCHAHNPKNNTFTSVYHTPLNLNVRTF PEAISGIHDQEDGEQCKSVFHWDMKSKDKEGAP NRQPLANERAYWTGYGEGNAWCPGALPDPEIVR MVEARKSLGEEYTEDYEQPRGKGSFPAMITPAYQ RAKKANQLASQVEYKRGHDERISRFSTVVDTPE LLRSKAGAQLQSDVRYTEDYEQQRGKGSFPAMI TPAYQIAKRANELASDVRYHQQYQKEMRGMAGPA IGAEGILTRECADQYGQGYPEEYEEHRGKGSFP AMITPAYQNAKKAHELASDIKYRQDFNKMKGAA HYHSLPAQDNLVLKQAQSVNKLVSEVEYKKDLES SRGHSINYCETPQFRNVSKISKFTSDNKYKENY QNHMRGRYEGVGMDRRTLHAMKVGSLASNVAYK ADYKHDIVDYNYPATLTPSYQTAMKLVPLKDANY RQSIDKLKYSSVTDTPQIVQAKINAQQLSHVNY RADYEKNKLNYTLPQDVPQLVKAKTNAKLFSEV KYKEGWEKTKGKGFEMKLDAMSLLAAKASGELAS NIKYKEEYEKTKGKAMGTADSRLLHSLQIAKMS SEVEYKKGFEESKTRFHLPMDMVNIRHAKKAQT LASDLDYRKKLHEYTVLPEDMKTQWAKKAYGLQS ELQYKADLAWMKGVGWLTEGSLNLEQAKKAGQL VSEKNYRQRVDELKFTSVTDSSQMEHAKKSQEL QSGVAYKAGNEQSVHQYTISKDEPLFLQARANAA NLSEKLYKSSWENQKAKGFELRLDSLTFLAAKA KRDLASEVKYKEDYERSRGKLIGAKDVQGDSQM SHSLQMSKLQSELEYKKGFEDTKSQCHVSLDMVH LVHARKAQHLATDVGYKTAEHHFTALPTDMKVE WAKKAYGLQSDNQYRADVKWMKGMGWVATGSLN VEQAKKAGELISEKKYRQHPDALKFTSIKDTPEM VQARISYTQAVDRLYREQGENIKHHYTPTADLP EVLLAKLNAMNISETRYKESWSKLRDGGYKLRL DALPFQAAKASGEIISDYKYKEAFEKMKGQMLGS RSLEDDISLAHSVYATSLQSDVNYKKGFEHSKA QFHLPLDMAALVHAKKAQTLASNQDYKHPLPQY TSLAEDLRLSCAKKAHKLQSENLYRSDLNFMRGV ACVIPGTLEIEGRKKASELISESKYRQHPHSFK YTAVTDTPNLLHAKFSNQITNERLYKAAGEDAR HEYTMTLGLPEFIRAKTNAANLSDARYKESWRNL RAQGYKLTIEALPFQAARASGDIASDFLYRHDF VKERGKLIGPQSVRDDPRIQHCRRMGQLQSELQ YRRGATSSQAQFHLPMDMVHLVHAKNAQALASDH DYRTQYHKFTALPEDLKMAWAKKAHALQSELRY KSDLIGMKGIGWLALRSPQMESAKKAGELISET KYRKKPDSIKFTTVVDSPDLVHAKNSYMHCNERM YRSGDAESLHRYTLIPDHPDFTRARLNALHLSD KVYRNSWEQTRAGSYDFRLDAIPFQTARASREI ASDFRYKEAFLRDRGLQIGYRSVDDDPRMKHFLN VGRLQSDNEYKKDFAKSRSQFHSSTDQPGLLQA KRSQQLASDVHYRQPLPQPTCDPEQLGLRHAQK AHQLQSDVKYKSDLNLTRGVGWTPPGSYKVEMAR RAAELANARGLGLQGAYRGAEAVEAGDHQSGEV NPDATEILHVKKKKALLL*AMSTLIPERPERKF VHQRQASDGFDFGKLEWPTSPSQIFLY*NNNSES RW