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Sequence NM_181689
TAGGTGGCGGGCGGGTACTTAAGGCGCGGCCACCGCGGCTGCGGCAGTGCGCCCAACAGCGGACTCCGAGACCAGCGGATCTCGGCAAACCCTCTTTCTCGACCACCCACCTACCATTCTTGGAACCATGGCGGCAGTGGCGGCGGCCTCGGCTGAACTGCTCATCATCGGCTGGTACATCTTCCGCGTGCTGCTGCAGG
TGTTCAGGTACTCCCTGCAGAAGCTGGCATACACGGTGTCGCGGACCGGGCGGCAGGTGTTGGGGGAGCGCAGGCAGCGAGCCCCCAACTGAGGCCCCAG
CTCCCAGCCCTGGGCGGCCGTATCATCAGGTGCTCCTGTGCATCTCGGCCAGCACGGGAGCCAGTGCCGCGCAGGAATGTGGGGTCCCCTGTGTTCCCTC
GCCAGAGGAGCACTTGGCAAGGTCAGTGAGGGGCCAGTAGACCCCCGGAGAAGCAGTACCGACAATGACGAAGATACCAGATCCCTTCCCAACCCCTTTG
CACCGGTCCCACTAAGGGGCAGGGTCGAGAGAGGAGGGGGGATAGGGGGAGCAGACCCCTGAGATCTGGGCATAGGCACCGCATTCTGATCTGGACAAAG
TCGGGACAGCACCATCCCAGCCCCGAAGCCAGGGCCATGCCAGCAGGCCCCACCATGGAAATCAAAACACCGCACCAGCCAGCAGAATGGACATTCTGAC
ATCGCCAGCCGACGCCCTGAATCTTGGTGCAGCACCAACCGCGTGCCTGTGTGGCGGGACTGGAGGGCACAGTTGAGGAAGGAGGGTGGTTAAGAAATAC
AGTGGGGCCCTCTCGCTGTCCCTTGCCCAGGGCACTTGCATTCCAGCCTCGCTGCATTTGCTCTCTCGATTCCCCTTTCCTCCTCACTGCCTCCCAAGCC
CACCCTACTCCAAAATAATGTGTCACTTGATTTGGAACTATTCAAGCAGTAAAAGTAAATGAATCCCACCTTTACTAAAACACTTTCTCTGAACCCCCCT
TGCCCCTCACTGATCTTGCTTTTCCCTGGTCTCATGCAGTTGTGGTCAATATTGTGGTAATCGCTAATTGTACTGATTGTTTAAGTGTGCATTAGTTGTG
TCTCCCCAGCTAGATTGTAAGCTCCTGGAGGACAGGGACCACCTCTACAAAAAATAAAAAAAGTACCTCCCCTGTCTCGCACAGTGTCCCAGGACCCTGC
GGTGCAGTAGAGGCGCACCAAAA
- refseq_NNAT.F1 (forward primer)
- refseq_NNAT.R1 (reverse primer)
- Single exon skipping
Frame 0
*VAGGYLRRGHRGCGSAPNSGLRDQRISANPLSRPPTYHSWNHGGSGGGLG*TAHHRLVHLPRAAAG
VQVLPAEAGIHGVADRAAGVGGAQAASPQLRPQ
LPALGGRIIRCSCASRPAREPVPRRNVGSPVFP
RQRSTWQGQ*GASRPPEKQYRQ*RRYQIPSQPLC
TGPTKGQGRERRGDRGSRPLRSGHRHRILIWTK
SGQHHPSPEARAMPAGPTMEIKTPHQPAEWTF*
HRQPTP*ILVQHQPRACVAGLEGTVEEGGWLRNT
VGPSRCPLPRALAFQPRCICSLDSPFLLTASQA
HPTPK*CVT*FGTIQAVKVNESHLY*NTFSEPP
LPLTDLAFPWSHAVVVNIVVIANCTDCLSVH*LC
LPS*IVSSWRTGTTSTKNKKSTSPVSHSVPGPC
GAVEAHQ
Frame 1
RWRAGT*GAATAAAAVRPTADSETSGSRQTLFLDHPPTILGTMAAVAAASAELLIIGWYIFRVLLQ
VFRYSLQKLAYTVSRTGRQVLGERRQRAPN*GPS
SQPWAAVSSGAPVHLGQHGSQCRAGMWGPLCSL
ARGALGKVSEGPVDPRRSSTDNDEDTRSLPNPF
APVPLRGRVERGGGIGGADP*DLGIGTAF*SGQS
RDSTIPAPKPGPCQQAPPWKSKHRTSQQNGHSD
IASRRPESWCSTNRVPVWRDWRAQLRKEGG*EI
QWGPLAVPCPGHLHSSLAAFALSIPLSSSLPPKP
TLLQNNVSLDLELFKQ*K*MNPTFTKTLSLNPP
CPSLILLFPGLMQLWSILW*SLIVLIV*VCISC
VSPARL*APGGQGPPLQKIKKVPPLSRTVSQDPA
VQ*RRTK
Frame 2
GGGRVLKARPPRLRQCAQQRTPRPADLGKPSFSTTHLPFLEPWRQWRRPRLNCSSSAGTSSACCCR
CSGTPCRSWHTRCRGPGGRCWGSAGSEPPTEAP
APSPGRPYHQVLLCISASTGASAAQECGVPCVPS
PEEHLARSVRGQ*TPGEAVPTMTKIPDPFPTPL
HRSH*GAGSREEGG*GEQTPEIWA*APHSDLDK
VGTAPSQPRSQGHASRPHHGNQNTAPASRMDILT
SPADALNLGAAPTACLCGGTGGHS*GRRVVKKY
SGALSLSLAQGTCIPASLHLLSRFPFPPHCLPS
PPYSKIMCHLIWNYSSSKSK*IPPLLKHFL*TPL
APH*SCFSLVSCSCGQYCGNR*LY*LFKCALVV
SPQLDCKLLEDRDHLYKK*KKYLPCLAQCPRTL
RCSRGAPK
Sequence NM_005386
TAGGTGGCGGGCGGGTACTTAAGGCGCGGCCACCGCGGCTGCGGCAGTGCGCCCAACAGCGGACTCCGAGACCAGCGGATCTCGGCAAACCCTCTTTCTCGACCACCCACCTACCATTCTTGGAACCATGGCGGCAGTGGCGGCGGCCTCGGCTGAACTGCTCATCATCGGCTGGTACATCTTCCGCGTGCTGCTGCAGG
TGTTCCTGGAATGCTGCATTTACTGGGTAGGATTCGCTTTTCGAAATCCTCCAGGGACACAGCCCATTGCGAGAAGTGAGGTGTTCAGGTACTCCCTGCA
GAAGCTGGCATACACGGTGTCGCGGACCGGGCGGCAGGTGTTGGGGGAGCGCAGGCAGCGAGCCCCCAACTGAGGCCCCAGCTCCCAGCCCTGGGCGGCC
GTATCATCAGGTGCTCCTGTGCATCTCGGCCAGCACGGGAGCCAGTGCCGCGCAGGAATGTGGGGTCCCCTGTGTTCCCTCGCCAGAGGAGCACTTGGCA
AGGTCAGTGAGGGGCCAGTAGACCCCCGGAGAAGCAGTACCGACAATGACGAAGATACCAGATCCCTTCCCAACCCCTTTGCACCGGTCCCACTAAGGGG
CAGGGTCGAGAGAGGAGGGGGGATAGGGGGAGCAGACCCCTGAGATCTGGGCATAGGCACCGCATTCTGATCTGGACAAAGTCGGGACAGCACCATCCCA
GCCCCGAAGCCAGGGCCATGCCAGCAGGCCCCACCATGGAAATCAAAACACCGCACCAGCCAGCAGAATGGACATTCTGACATCGCCAGCCGACGCCCTG
AATCTTGGTGCAGCACCAACCGCGTGCCTGTGTGGCGGGACTGGAGGGCACAGTTGAGGAAGGAGGGTGGTTAAGAAATACAGTGGGGCCCTCTCGCTGT
CCCTTGCCCAGGGCACTTGCATTCCAGCCTCGCTGCATTTGCTCTCTCGATTCCCCTTTCCTCCTCACTGCCTCCCAAGCCCACCCTACTCCAAAATAAT
GTGTCACTTGATTTGGAACTATTCAAGCAGTAAAAGTAAATGAATCCCACCTTTACTAAAACACTTTCTCTGAACCCCCCTTGCCCCTCACTGATCTTGC
TTTTCCCTGGTCTCATGCAGTTGTGGTCAATATTGTGGTAATCGCTAATTGTACTGATTGTTTAAGTGTGCATTAGTTGTGTCTCCCCAGCTAGATTGTA
AGCTCCTGGAGGACAGGGACCACCTCTACAAAAAATAAAAAAAGTACCTCCCCTGTCTCGCACAGTGTCCCAGGACCCTGCGGTGCAGTAGAGGCGCACC
AAAA
- refseq_NNAT.F1 (forward primer)
- refseq_NNAT.R1 (reverse primer)
- Single exon skipping
Frame 0
*VAGGYLRRGHRGCGSAPNSGLRDQRISANPLSRPPTYHSWNHGGSGGGLG*TAHHRLVHLPRAAAG
VPGMLHLLGRIRFSKSSRDTAHCEK*GVQVLPA
EAGIHGVADRAAGVGGAQAASPQLRPQLPALGG
RIIRCSCASRPAREPVPRRNVGSPVFPRQRSTWQ
GQ*GASRPPEKQYRQ*RRYQIPSQPLCTGPTKG
QGRERRGDRGSRPLRSGHRHRILIWTKSGQHHP
SPEARAMPAGPTMEIKTPHQPAEWTF*HRQPTP*
ILVQHQPRACVAGLEGTVEEGGWLRNTVGPSRC
PLPRALAFQPRCICSLDSPFLLTASQAHPTPK*
CVT*FGTIQAVKVNESHLY*NTFSEPPLPLTDLA
FPWSHAVVVNIVVIANCTDCLSVH*LCLPS*IV
SSWRTGTTSTKNKKSTSPVSHSVPGPCGAVEAH
Q
Frame 1
RWRAGT*GAATAAAAVRPTADSETSGSRQTLFLDHPPTILGTMAAVAAASAELLIIGWYIFRVLLQ
VFLECCIYWVGFAFRNPPGTQPIARSEVFRYSLQ
KLAYTVSRTGRQVLGERRQRAPN*GPSSQPWAA
VSSGAPVHLGQHGSQCRAGMWGPLCSLARGALG
KVSEGPVDPRRSSTDNDEDTRSLPNPFAPVPLRG
RVERGGGIGGADP*DLGIGTAF*SGQSRDSTIP
APKPGPCQQAPPWKSKHRTSQQNGHSDIASRRP
ESWCSTNRVPVWRDWRAQLRKEGG*EIQWGPLAV
PCPGHLHSSLAAFALSIPLSSSLPPKPTLLQNN
VSLDLELFKQ*K*MNPTFTKTLSLNPPCPSLIL
LFPGLMQLWSILW*SLIVLIV*VCISCVSPARL*
APGGQGPPLQKIKKVPPLSRTVSQDPAVQ*RRT
K
Frame 2
GGGRVLKARPPRLRQCAQQRTPRPADLGKPSFSTTHLPFLEPWRQWRRPRLNCSSSAGTSSACCCR
CSWNAAFTG*DSLFEILQGHSPLREVRCSGTPC
RSWHTRCRGPGGRCWGSAGSEPPTEAPAPSPGRP
YHQVLLCISASTGASAAQECGVPCVPSPEEHLA
RSVRGQ*TPGEAVPTMTKIPDPFPTPLHRSH*G
AGSREEGG*GEQTPEIWA*APHSDLDKVGTAPSQ
PRSQGHASRPHHGNQNTAPASRMDILTSPADAL
NLGAAPTACLCGGTGGHS*GRRVVKKYSGALSL
SLAQGTCIPASLHLLSRFPFPPHCLPSPPYSKIM
CHLIWNYSSSKSK*IPPLLKHFL*TPLAPH*SC
FSLVSCSCGQYCGNR*LY*LFKCALVVSPQLDC
KLLEDRDHLYKK*KKYLPCLAQCPRTLRCSRGAP
K
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