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Sequence NM_000269
GCAGAAGCGTTCCGTGCGTGCAAGTGCTGCGAACCACGTGGGTCCCGGGCGCGTTTCGGGTGCTGGCGGCTGCAGCCGGAGTTCAAACCTAAGCAGCTGGAAGGAACCATGGCCAACTGTGAGCGTACCTTCATTGCGATCAAACCAGATGGGGTCCAGCGGGGTCTTGTGGGAGAGATTATCAAGCGTTTTGAGCAGAA
AGGATTCCGCCTTGTTGGTCTGAAATTCATGCAAGCTTCCGAAGATCTTCTCAAGGAACACTACGTTGACCTGAAGGACCGTCCATTCTTTGCCGGCCTG
GTGAAATACATGCACTCAGGGCCGGTAGTTGCCATGGTCTGGGAGGGGCTGAATGTGGTGAAGACGGGCCGAGTCATGCTCGGGGAGACCAACCCTGCAG
ACTCCAAGCCTGGGACCATCCGTGGAGACTTCTGCATACAAGTTGGCAGGAACATTATACATGGCAGTGATTCTGTGGAGAGTGCAGAGAAGGAGATCGG
CTTGTGGTTTCACCCTGAGGAACTGGTAGATTACACGAGCTGTGCTCAGAACTGGATCTATGAATGACAGGAGGGCAGACCACATTGCTTTTCACATCCA
TTTCCCCTCCTTCCCATGGGCAGAGGACCAGGCTGTAGGAAATCTAGTTATTTACAGGAACTTCATCATAATTTGGAGGGAAGCTCTTGGAGCTGTGAGT
TCTCCCTGTACAGTGTTACCATCCCCGACCATCTGATTAAAATGCTTCCTCCCAGCATAGGATTCATTGAGTTGGTTACTTCATATTGTTGCATTGCTTT
TTTTTCCTTCT
- refseq_NME1.F2 (forward primer)
- refseq_NME1.R2 (reverse primer)
- Single exon skipping
Frame 0
AEAFRACKCCEPRGSRARFGCWRLQPEFKPKQLEGTMANCERTFIAIKPDGVQRGLVGEIIKRFEQK
GFRLVGLKFMQASEDLLKEHYVDLKDRPFFAGL
VKYMHSGPVVAMVWEGLNVVKTGRVMLGETNPA
DSKPGTIRGDFCIQVGRNIIHGSDSVESAEKEIG
LWFHPEELVDYTSCAQNWIYE*QEGRPHCFSHP
FPLLPMGRGPGCRKSSYLQELHHNLEGSSWSCE
FSLYSVTIPDHLIKMLPPSIGFIELVTSYCCIAF
FSF
Frame 1
QKRSVRASAANHVGPGRVSGAGGCSRSSNLSSWKEPWPTVSVPSLRSNQMGSSGVLWERLSSVLSR
KDSALLV*NSCKLPKIFSRNTTLT*RTVHSLPAW
*NTCTQGR*LPWSGRG*MW*RRAESCSGRPTLQ
TPSLGPSVETSAYKLAGTLYMAVILWRVQRRRS
ACGFTLRNW*ITRAVLRTGSMNDRRADHIAFHIH
FPSFPWAEDQAVGNLVIYRNFIIIWREALGAVS
SPCTVLPSPTI*LKCFLPA*DSLSWLLHIVALL
FFPS
Frame 2
RSVPCVQVLRTTWVPGAFRVLAAAAGVQT*AAGRNHGQL*AYLHCDQTRWGPAGSCGRDYQAF*AE
RIPPCWSEIHASFRRSSQGTLR*PEGPSILCRP
GEIHALRAGSCHGLGGAECGEDGPSHARGDQPCR
LQAWDHPWRLLHTSWQEHYTWQ*FCGECREGDR
LVVSP*GTGRLHELCSELDL*MTGGQTTLLFTS
ISPPSHGQRTRL*EI*LFTGTSS*FGGKLLEL*V
LPVQCYHPRPSD*NASSQHRIH*VGYFILLHCF
FFL
Sequence NM_001018136
GCAGAAGCGTTCCGTGCGTGCAAGTGCTGCGAACCACGTGGGTCCCGGGCGCGTTTCGGGTGCTGGCGGCTGCAGCCGGAGTTCAAACCTAAGCAGCTGGAAGGAACCATGGCCAACTGTGAGCGTACCTTCATTGCGATCAAACCAGATGGGGTCCAGCGGGGTCTTGTGGGAGAGATTATCAAGCGTTTTGAGCAGAA
AGGATTCCGCCTTGTTGGTCTGAAATTCATGCAAGCTTCCGAAGATCTTCTCAAGGAACACTACGTTGACCTGAAGGACCGTCCATTCTTTGCCGGCCTG
GTGAAATACATGCACTCAGGGCCGGTAGTTGCCATGGTCTGGGAGGGGCTGAATGTGGTGAAGACGGGCCGAGTCATGCTCGGGGAGACCAACCCTGCAG
ACTCCAAGCCTGGGACCATCCGTGGAGACTTCTGCATACAAGTTGGCAGGACCATGGCCAACCTGGAGCGCACCTTCATCGCCATCAAGCCGGACGGCGT
GCAGCGCGGCCTGGTGGGCGAGATCATCAAGCGCTTCGAGCAGAAGGGATTCCGCCTCGTGGCCATGAAGTTCCTCCGGGCCTCTGAAGAACACCTGAAG
CAGCACTACATTGACCTGAAAGACCGACCATTCTTCCCTGGGCTGGTGAAGTACATGAACTCAGGGCCGGTTGTGGCCATGGTCTGGGAGGGGCTGAACG
TGGTGAAGACAGGCCGAGTGATGCTTGGGGAGACCAATCCAGCAGATTCAAAGCCAGGCACCATTCGTGGGGACTTCTGCATTCAGGTTGGCAGGAACAT
CATTCATGGCAGTGATTCAGTAAAAAGTGCTGAAAAAGAAATCAGCCTATGGTTTAAGCCTGAAGAACTGGTTGACTACAAGTCTTGTGCTCATGACTGG
GTCTATGAATAAGAGGTGGACACAACAGCAGTCTCCTTCAGCACGGCGTGGTGTGTCCCTGGACACAGCTCTTCATTCCATTGACTTAGAGGCAACAGGA
TTGATCATTCTTTTATAGAGCATATTTGCCAATAAAGCTTTTGGAAGCCGGA
- refseq_NME1.F2 (forward primer)
- refseq_NME1.R2 (reverse primer)
- Single exon skipping
Frame 0
AEAFRACKCCEPRGSRARFGCWRLQPEFKPKQLEGTMANCERTFIAIKPDGVQRGLVGEIIKRFEQK
GFRLVGLKFMQASEDLLKEHYVDLKDRPFFAGL
VKYMHSGPVVAMVWEGLNVVKTGRVMLGETNPA
DSKPGTIRGDFCIQVGRTMANLERTFIAIKPDGV
QRGLVGEIIKRFEQKGFRLVAMKFLRASEEHLK
QHYIDLKDRPFFPGLVKYMNSGPVVAMVWEGLN
VVKTGRVMLGETNPADSKPGTIRGDFCIQVGRNI
IHGSDSVKSAEKEISLWFKPEELVDYKSCAHDW
VYE*EVDTTAVSFSTAWCVPGHSSSFH*LRGNR
IDHSFIEHICQ*SFWKP
Frame 1
QKRSVRASAANHVGPGRVSGAGGCSRSSNLSSWKEPWPTVSVPSLRSNQMGSSGVLWERLSSVLSR
KDSALLV*NSCKLPKIFSRNTTLT*RTVHSLPAW
*NTCTQGR*LPWSGRG*MW*RRAESCSGRPTLQ
TPSLGPSVETSAYKLAGPWPTWSAPSSPSSRTA
CSAAWWARSSSASSRRDSASWP*SSSGPLKNT*S
STTLT*KTDHSSLGW*ST*TQGRLWPWSGRG*T
W*RQAE*CLGRPIQQIQSQAPFVGTSAFRLAGT
SFMAVIQ*KVLKKKSAYGLSLKNWLTTSLVLMTG
SMNKRWTQQQSPSARRGVSLDTALHSIDLEATG
LIILL*SIFANKAFGSR
Frame 2
RSVPCVQVLRTTWVPGAFRVLAAAAGVQT*AAGRNHGQL*AYLHCDQTRWGPAGSCGRDYQAF*AE
RIPPCWSEIHASFRRSSQGTLR*PEGPSILCRP
GEIHALRAGSCHGLGGAECGEDGPSHARGDQPCR
LQAWDHPWRLLHTSWQDHGQPGAHLHRHQAGRR
AARPGGRDHQALRAEGIPPRGHEVPPGL*RTPE
AALH*PERPTILPWAGEVHELRAGCGHGLGGAER
GEDRPSDAWGDQSSRFKARHHSWGLLHSGWQEH
HSWQ*FSKKC*KRNQPMV*A*RTG*LQVLCS*L
GL*IRGGHNSSLLQHGVVCPWTQLFIPLT*RQQD
*SFFYRAYLPIKLLEAG
Sequence NM_198175
GCAGAAGCGTTCCGTGCGTGCAAGTGCTGCGAACCACGTGGGTCCCGGGCGCGTTTCGGGTGCTGGCGGCTGCAGCCGGAGTTCAAACCTAAGCAGCTGGAAGGGCCCTGTGGCTAGGTACCATAGAGTCTCTACACAGGACTAAGTCAGCCTGGTGTGCAGGGGAGGCAGACACACAAACAGAAAATTGGACTACAGTG
CTAAGATGCTGTAAGAAGAGGTTAACTAAAGGACAGGAAGATGGGGCCAAGAGATGGTGCTACTGTCTACTTTAGGGATCGTCTTTCAAGGCGAGGGGCC
TCCTATCTCAAGCTGTGATACAGGAACCATGGCCAACTGTGAGCGTACCTTCATTGCGATCAAACCAGATGGGGTCCAGCGGGGTCTTGTGGGAGAGATT
ATCAAGCGTTTTGAGCAGAAAGGATTCCGCCTTGTTGGTCTGAAATTCATGCAAGCTTCCGAAGATCTTCTCAAGGAACACTACGTTGACCTGAAGGACC
GTCCATTCTTTGCCGGCCTGGTGAAATACATGCACTCAGGGCCGGTAGTTGCCATGGTCTGGGAGGGGCTGAATGTGGTGAAGACGGGCCGAGTCATGCT
CGGGGAGACCAACCCTGCAGACTCCAAGCCTGGGACCATCCGTGGAGACTTCTGCATACAAGTTGGCAGGAACATTATACATGGCAGTGATTCTGTGGAG
AGTGCAGAGAAGGAGATCGGCTTGTGGTTTCACCCTGAGGAACTGGTAGATTACACGAGCTGTGCTCAGAACTGGATCTATGAATGACAGGAGGGCAGAC
CACATTGCTTTTCACATCCATTTCCCCTCCTTCCCATGGGCAGAGGACCAGGCTGTAGGAAATCTAGTTATTTACAGGAACTTCATCATAATTTGGAGGG
AAGCTCTTGGAGCTGTGAGTTCTCCCTGTACAGTGTTACCATCCCCGACCATCTGATTAAAATGCTTCCTCCCAGCATAGGATTCATTGAGTTGGTTACT
TCATATTGTTGCATTGCTTTTTTTTCCTTCT
- refseq_NME1.F2 (forward primer)
- refseq_NME1.R2 (reverse primer)
- Single exon skipping
Frame 0
AEAFRACKCCEPRGSRARFGCWRLQPEFKPKQLEGPCG*VP*SLYTGLSQPGVQGRQTHKQKIGLQC
*DAVRRG*LKDRKMGPRDGATVYFRDRLSRRGA
SYLKL*YRNHGQL*AYLHCDQTRWGPAGSCGRD
YQAF*AERIPPCWSEIHASFRRSSQGTLR*PEGP
SILCRPGEIHALRAGSCHGLGGAECGEDGPSHA
RGDQPCRLQAWDHPWRLLHTSWQEHYTWQ*FCG
ECREGDRLVVSP*GTGRLHELCSELDL*MTGGQT
TLLFTSISPPSHGQRTRL*EI*LFTGTSS*FGG
KLLEL*VLPVQCYHPRPSD*NASSQHRIH*VGY
FILLHCFFFL
Frame 1
QKRSVRASAANHVGPGRVSGAGGCSRSSNLSSWKGPVARYHRVSTQD*VSLVCRGGRHTNRKLDYS
AKML*EEVN*RTGRWGQEMVLLSTLGIVFQGEGP
PISSCDTGTMANCERTFIAIKPDGVQRGLVGEI
IKRFEQKGFRLVGLKFMQASEDLLKEHYVDLKD
RPFFAGLVKYMHSGPVVAMVWEGLNVVKTGRVML
GETNPADSKPGTIRGDFCIQVGRNIIHGSDSVE
SAEKEIGLWFHPEELVDYTSCAQNWIYE*QEGR
PHCFSHPFPLLPMGRGPGCRKSSYLQELHHNLEG
SSWSCEFSLYSVTIPDHLIKMLPPSIGFIELVT
SYCCIAFFSF
Frame 2
RSVPCVQVLRTTWVPGAFRVLAAAAGVQT*AAGRALWLGTIESLHRTKSAWCAGEADTQTENWTTV
LRCCKKRLTKGQEDGAKRWCYCLL*GSSFKARG
LLSQAVIQEPWPTVSVPSLRSNQMGSSGVLWERL
SSVLSRKDSALLV*NSCKLPKIFSRNTTLT*RT
VHSLPAW*NTCTQGR*LPWSGRG*MW*RRAESC
SGRPTLQTPSLGPSVETSAYKLAGTLYMAVILWR
VQRRRSACGFTLRNW*ITRAVLRTGSMNDRRAD
HIAFHIHFPSFPWAEDQAVGNLVIYRNFIIIWR
EALGAVSSPCTVLPSPTI*LKCFLPA*DSLSWLL
HIVALLFFPS
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