Sequences - Rnomics 📊 Palace

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Sequences

Sequence NM_002401

GAGAACGGTGGCCGGACGGAGAGACTGCGGGTCTGAGGGACTGGCGGGCGGGCGGGCCGAGCGGCGCCGCCGAGGCCGGGCTGGGCCGAGCCCAGGAGCG CCCGGGATGTAGCGGGCCACCCTGCCGATGCCACAGCGCCCGGCCGCGGGCGGAGCCGGAGCCGGAGCCTGGGGAGGCGGCGGGGGCCCAGAGCGCAGCC CGCGCCCCCCGCGCGGAGCCAGGCCCGCTGCCGTCCCCGCCGCCCGGGCCCCCGGCATGCAGCCCCGGCTGCGGAGGTGACACTCACGGACCTTAGCCAC CGCCGCCGCCATCGCCACCATGGACGAACAGGAGGCATTGAACTCAATCATGAACGATCTGGTGGCCCTCCAGATGAACCGACGTCACCGGATGCCTGGA TATGAGACCATGAAGAACAAAGACACAGGTCACTCAAATAGGCAGAGTGACGTCAGAATCAAGTTCGAGCACAACGGGGAGAGGCGAATTATAGCGTTCA GCCGGCCTGTGAAATATGAAGATGTGGAGCACAAGGTGACAACAGTATTTGGACAACCTCTTGATCTACATTACATGAACAATGAGCTCTCCATCCTGCT GAAAAACCAAGATGATCTTGATAAAGCAATTGACATTTTAGATAGAAGCTCAAGCATGAAAAGCCTTAGGATATTGCTGTTGTCCCAGGACAGAAACCAT AACAGTTCCTCTCCCCACTCTGGGGTGTCCAGACAGGTGCGGATCAAGGCTTCCCAGTCCGCAGGGGATATAAATACTATCTACCAGCCCCCCGAGCCCA GAAGCAGGCACCTCTCTGTCAGCTCCCAGAACCCTGGCCGAAGCTCACCTCCCCCTGGCTATGTTCCTGAGCGGCAGCAGCACATTGCCCGGCAGGGGTC CTACACCAGCATCAACAGTGAGGGGGAGTTCATCCCAGAGACCAGCGAGCAGTGCATGCTGGATCCCCTGAGCAGTGCAGAAAATTCCTTGTCTGGAAGC TGCCAATCCTTGGACAGGTCAGCAGACAGCCCATCCTTCCGGAAATCACGAATGTCCCGTGCCCAGAGCTTCCCTGACAACAGACAGGAATACTCAGATC GGGAAACTCAGCTTTATGACAAAGGGGTCAAAGGTGGAACCTACCCCCGGCGCTACCACGTGTCTGTGCACCACAAGGACTACAGTGATGGCAGAAGAAC ATTTCCCCGAATACGGCGTCATCAAGGCAACTTGTTCACCCTGGTGCCCTCCAGCCGCTCCCTGAGCACAAATGGCGAGAACATGGGTCTGGCTGTGCAA TACCTGGACCCCCGTGGGCGCCTGCGGAGTGCGGACAGCGAGAATGCCCTCTCTGTGCAGGAGAGGAATGTGCCAACCAAGTCTCCCAGTGCCCCCATCA ACTGGCGCCGGGGAAAGCTCCTGGGCCAGGGTGCCTTCGGCAGGGTCTATTTGTGCTATGACGTGGACACGGGACGTGAACTTGCTTCCAAGCAGGTCCA ATTTGATCCAGACAGTCCTGAGACAAGCAAGGAGGTGAGTGCTCTGGAGTGCGAGATCCAGTTGCTAAAGAACTTGCAGCATGAGCGCATCGTGCAGTAC TATGGCTGTCTGCGGGACCGCGCTGAGAAGACCCTGACCATCTTCATGGAGTACATGCCAGGGGGCTCGGTGAAAGACCAGTTGAAGGCTTACGGTGCTC TGACAGAGAGCGTGACCCGAAAGTACACGCGGCAGATCCTGGAGGGCATGTCCTACCTGCACAGCAACATGATTGTTCACCGGGACATTAAGGGAGCCAA CATCCTCCGAGACTCTGCTGGGAATGTAAAGCTGGGGGACTTTGGGGCCAGCAAACGCCTGCAGACGATCTGTATGTCGGGGACGGGCATGCGCTCCGTC ACTGGCACACCCTACTGGATGAGCCCTGAGGTGATCAGCGGCGAGGGCTATGGAAGGAAAGCAGACGTGTGGAGCCTGGGCTGCACTGTGGTGGAGATGC TGACAGAGAAACCACCGTGGGCAGAGTATGAAGCTATGGCCGCCATCTTCAAGATTGCCACCCAGCCCACCAATCCTCAGCTGCCCTCCCACATCTCTGA ACATGGCCGGGACTTCCTGAGGCGCATTTTTGTGGAGGCTCGCCAGAGACCTTCAGCTGAGGAGCTGCTCACACACCACTTTGCACAGCTCATGTACTGA GCTCTCACGGCCACACAGCTGCCGGTCGCCCTTTGCTGCATGGCAGGGGGCTGCTGCTGGGCTCAGTGAAGTTGCTGCTTCTCCCAGGCAAGGCTGTGGA CCATGGAGTGGCAGCCCAGCCAGCGTCGGTCTGTGCCCCTTCCGCCACTGGGGCTCAGAGCCGGGGTGGGGTGGCTGCAGCCTCAGGACTGGGAGCCCCC AGCCTGTCAGATCCAGGAGCTCCAGTGTCCTGAGCTCAGCGTGGAGGGGTAGGGGCTGGGAACAGTGTGCAAGGCAGCCGTGGGCCCCACCCTCGGGGAT GTGTCCTGACACTGCAATTGGCACCGAAGCCCAGAGGGTCTGGGGGCACAAGACTGACGCCAGGGTATGAAGAGTGTTATTTTCATTCAAAGTGTTATTT TGTTTTTCCTTCCAATGTCTGGAGACCACCAGGGCATCTCTGGGCTGGATGAGCTCCCACAAGCCTGAGGGAAAGGCCAGCACTCGCTAGCAGTGGCAGG CAGAGGCCCAGGCTGCCGTCCCCTAGAGTCCCAGGTTGGCTCTGCCAGTCCTGTCCTTTACCAAAGATGAATGAAGCAAATGTCATGCTGCCTTATTCAG GGAAGGAGGAGCCTGTCCTGCCTGTGGCCATGACCCTGCCTCTCCCAGGCAGGGGCCCGCGATGTGGAACTGCTGCCACTGAGGGGGGATCCAGTTTTGT CAATGCAGTTGTCTCTGTTTTACAAGTTGGAGTCACTCTTATGCTGTACCCAGTTTCTAAACTGGAGACTGTGTGTGCCCTCTGGGCTCTGAGTACCCCT GCTTTGGGCTTGGGCCTAGGCTGCATTGAAAAGAGCTGAAGGTTGTGGCCTTTGCGCTCCTGGCCCAGCCTTTGTTCCCCACTGGAGCAGAAGGGGAGAT GGACGACACGGTCGGGGCATCTGGCCTGGCCAGTGCCCTGATCCCAGAGAGCCCGAGGAGGTGTCTCAGGCTGCCTGAGTCGTGACCTGCTAGGCCAGAG CCCACTCCATCTGGTAGAAGGGAAAGCCCATATGCTACCACCAGCTGTGTCCAAAACCGCCAGCTCTGTTCTTCCTCAGCCAGCCTCGCCCATCCCCTTG AGGTCTCAGCCCCTTTCCCTTGTAGCTCCTCCCCTGGAGGGGGAATGGCAGCAGGGGTTGGGGAAACAGCATCTCCAAGCAGCTTAGAGTTGGCCATATT TACCTCAGCCTGGGCGCTGGTCCTTTCTTCCGGCCCCTCCCCTCCAAAATGTGCCTATTGCTAGAGCTCCTCCCTCTCAACACCCAGTTTCCTTGGGAGT TGTCATTAAAGGAAAAAAAAAAAAAAAAAAGCCAGTGCCCAGGGATGGGCATCTCCAGGGAGCTGGGGATTAGTGCCAGGCAGCCCTGCCAGCCATGCCT ACATCCCCATGGGCACAGAACAAGCCAAAGCCTTCGTTGTATGTTGACGATGCACTTTTATGAATGTAGTTTCTATCGCTGTTTTTAGCCTTTTCACATC ATGTAATGTGAGGCCTTGTACTTGTTAATTTATATCTCAGATCATATTTGATGGTTTTTATATATATCAATTCTAGACTGTTACAGGTGACGGACGCCTC AAGAGAGAGAAGAGAAAATGAAAGCAGCTGGTTTTGCAGAAGTGTGTGTCGCATGCGCCAGTTGGGCCTGGACCCTCCTGTGTCCATCCCTGTTCCCCCA GGGGCTCTATCAGCCCCTGTACCCCACACTGCCCTCTGAAGACAACACAGGCTCCTGCTTCCACCTCGGCCCTTGCCCAGGGTGGGGCCTGGCCCTCATC TTGACCAAAGCTGCTGTGTGGCAGCTCGGCCTCTCTACGACCCCATCTTGGTGGCTGCACACTCTTCCTGGCCCGCACCCCCATCCCCAGTCCCTGTTCC CCAAGAGGATACAGAGCACGGTGCTGGCTGACTCAACTGTGCGTCCCAGGTTCAGGGTCTTACAGAGCTCCACCCCCTGGGGTCTTACCTCACTGGGAAT GTGTTTTGAAAATGAATTTGAAGACAAGCCAACAAACCCTGCACTCCAAAAAAGCAAAACAGACCCTAATTTTTTTGTGCCAAAAACTGTGGACATGCTG GCTCAGCATCCTCAGGACCAAGTTGTTGCTTAATTTATTGTTTTTTAATAACTAATCCAGATAAAAAGTTGTGGGGCTTCAGGGTGACCTGGGCCCAAAG GTTCTGAAGGGCAGTTCCTGGCAGCCCCAGGCTTGCTGTGGGAAGGGGCCGTGCCGTCACTTTCTCATCATTCCATGGGGTGTGTCTGCCTGGGCCAACT CTGCATGGAGAGGCCAGGGCTGGGGACAGTCCGCACTCTGCCACCCTCCTGCCCCTTCCACCCACCCCAGCTCTATGTCTGTGTCTGAATTGTGGATCGT GCAGCCATGGTTATTGTGGAACTGTGGAACCTGCAGCCATAGTTATTTGACTATATCTTGACCGAGGGCTTGCAGTGCAAAGCCAGGCCAGTGTTGCGCA TTACTTACAATAAAAGGGATCATTTATATCAGA

refseq_MAP3K3.F2 (forward primer)
refseq_MAP3K3.R2 (reverse primer)
Single exon skipping

Frame 0

ENGGRTERLRV*GTGGRAGRAAPPRPGWAEPRS ARDVAGHPADATAPGRGRSRSRSLGRRRGPRAQP APPARSQARCRPRRPGPRHAAPAAEVTLTDLSH RRRHRHHGRTGGIELNHERSGGPPDEPTSPDAW I*DHEEQRHRSLK*AE*RQNQVRAQRGEANYSVQ PACEI*RCGAQGDNSIWTTS*STLHEQ*ALHPA EKPR*S**SN*HFR*KLKHEKP*DIAVVPGQKP *QFLSPLWGVQTGADQGFPVRRGYKYYLPAPRAQ KQAPLCQLPEPWPKLTSPWLCS*AAAAHCPAGV LHQHQQ*GGVHPRDQRAVHAGSPEQCRKFLVWK LPILGQVSRQPILPEITNVPCPELP*QQTGILRS GNSAL*QRGQRWNLPPALPRVCAPQGLQ*WQKN ISPNTASSRQLVHPGALQPLPEHKWREHGSGCA IPGPPWAPAECGQRECPLCAGEECANQVSQCPHQ LAPGKAPGPGCLRQGLFVL*RGHGT*TCFQAGP I*SRQS*DKQGGECSGVRDPVAKELAA*AHRAV LWLSAGPR*EDPDHLHGVHARGLGERPVEGLRCS DRERDPKVHAADPGGHVLPAQQHDCSPGH*GSQ HPPRLCWECKAGGLWGQQTPADDLYVGDGHALR HWHTLLDEP*GDQRRGLWKESRRVEPGLHCGGDA DRETTVGRV*SYGRHLQDCHPAHQSSAALPHL* TWPGLPEAHFCGGSPETFS*GAAHTPLCTAHVL SSHGHTAAGRPLLHGRGLLLGSVKLLLLPGKAVD HGVAAQPASVCAPSATGAQSRGGVAAASGLGAP SLSDPGAPVS*AQRGGVGAGNSVQGSRGPHPRG CVLTLQLAPKPRGSGGTRLTPGYEECYFHSKCYF VFPSNVWRPPGHLWAG*APTSLRERPALASSGR QRPRLPSPRVPGWLCQSCPLPKMNEANVMLPYS GKEEPVLPVAMTLPLPGRGPRCGTAATEGGSSFV NAVVSVLQVGVTLMLYPVSKLETVCALWALSTP ALGLGLGCIEKS*RLWPLRSWPSLCSPLEQKGR WTTRSGHLAWPVP*SQRARGGVSGCLSRDLLGQS PLHLVEGKAHMLPPAVSKTASSVLPQPASPIPL RSQPLSLVAPPLEGEWQQGLGKQHLQAA*SWPY LPQPGRWSFLPAPPLQNVPIARAPPSQHPVSLGV VIKGKKKKKKPVPRDGHLQGAGD*CQAALPAMP TSPWAQNKPKPSLYVDDALL*M*FLSLFLAFSH HVM*GLVLVNLYLRSYLMVFIYINSRLLQVTDAS RERRENESSWFCRSVCRMRQLGLDPPVSIPVPP GALSAPVPHTAL*RQHRLLLPPRPLPRVGPGPH LDQSCCVAARPLYDPILVAAHSSWPAPPSPVPVP QEDTEHGAG*LNCASQVQGLTELHPLGSYLTGN VF*K*I*RQANKPCTPKKQNRP*FFCAKNCGHA GSASSGPSCCLIYCFLITNPDKKLWGFRVTWAQR F*RAVPGSPRLAVGRGRAVTFSSFHGVCLPGPT LHGEARAGDSPHSATLLPLPPTPALCLCLNCGS CSHGYCGTVEPAAIVI*LYLDRGLAVQSQASVAH YLQ*KGSFIS

Frame 1

RTVAGRRDCGSEGLAGGRAERRRRGRAGPSPGA PGM*RATLPMPQRPAAGGAGAGAWGGGGGPERS PRPPRGARPAAVPAARAPGMQPRLRR*HSRTLAT AAAIATMDEQEALNSIMNDLVALQMNRRHRMPG YETMKNKDTGHSNRQSDVRIKFEHNGERRIIAF SRPVKYEDVEHKVTTVFGQPLDLHYMNNELSILL KNQDDLDKAIDILDRSSSMKSLRILLLSQDRNH NSSSPHSGVSRQVRIKASQSAGDINTIYQPPEP RSRHLSVSSQNPGRSSPPPGYVPERQQHIARQGS YTSINSEGEFIPETSEQCMLDPLSSAENSLSGS CQSLDRSADSPSFRKSRMSRAQSFPDNRQEYSD RETQLYDKGVKGGTYPRRYHVSVHHKDYSDGRRT FPRIRRHQGNLFTLVPSSRSLSTNGENMGLAVQ YLDPRGRLRSADSENALSVQERNVPTKSPSAPI NWRRGKLLGQGAFGRVYLCYDVDTGRELASKQVQ FDPDSPETSKEVSALECEIQLLKNLQHERIVQY YGCLRDRAEKTLTIFMEYMPGGSVKDQLKAYGA LTESVTRKYTRQILEGMSYLHSNMIVHRDIKGAN ILRDSAGNVKLGDFGASKRLQTICMSGTGMRSV TGTPYWMSPEVISGEGYGRKADVWSLGCTVVEM LTEKPPWAEYEAMAAIFKIATQPTNPQLPSHISE HGRDFLRRIFVEARQRPSAEELLTHHFAQLMY* ALTATQLPVALCCMAGGCCWAQ*SCCFSQARLW TMEWQPSQRRSVPLPPLGLRAGVGWLQPQDWEPP ACQIQELQCPELSVEG*GLGTVCKAAVGPTLGD VS*HCNWHRSPEGLGAQD*RQGMKSVIFIQSVI LFFLPMSGDHQGISGLDELPQA*GKGQHSLAVAG RGPGCRPLESQVGSASPVLYQR*MKQMSCCLIQ GRRSLSCLWP*PCLSQAGARDVELLPLRGDPVL SMQLSLFYKLESLLCCTQFLNWRLCVPSGL*VPL LWAWA*AALKRAEGCGLCAPGPAFVPHWSRRGD GRHGRGIWPGQCPDPREPEEVSQAA*VVTC*AR AHSIW*KGKPICYHQLCPKPPALFFLSQPRPSP* GLSPFPL*LLPWRGNGSRGWGNSISKQLRVGHI YLSLGAGPFFRPLPSKMCLLLELLPLNTQFPWE LSLKEKKKKKSQCPGMGISRELGISARQPCQPCL HPHGHRTSQSLRCMLTMHFYECSFYRCF*PFHI M*CEALYLLIYISDHI*WFLYISILDCYR*RTP QEREEKMKAAGFAEVCVACASWAWTLLCPSLFPQ GLYQPLYPTLPSEDNTGSCFHLGPCPGWGLALI LTKAAVWQLGLSTTPSWWLHTLPGPHPHPQSLF PKRIQSTVLADSTVRPRFRVLQSSTPWGLTSLGM CFENEFEDKPTNPALQKSKTDPNFFVPKTVDML AQHPQDQVVA*FIVF**LIQIKSCGASG*PGPK GSEGQFLAAPGLLWEGAVPSLSHHSMGCVCLGQL CMERPGLGTVRTLPPSCPFHPPQLYVCV*IVDR AAMVIVELWNLQP*LFDYILTEGLQCKARPVLR ITYNKRDHLYQ

Frame 2

ERWPDGETAGLRDWRAGGPSGAAEAGLGRAQER PGCSGPPCRCHSARPRAEPEPEPGEAAGAQSAA RAPRAEPGPLPSPPPGPPACSPGCGGDTHGP*P PPPPSPPWTNRRH*TQS*TIWWPSR*TDVTGCLD MRP*RTKTQVTQIGRVTSESSSSTTGRGEL*RS AGL*NMKMWSTR*QQYLDNLLIYIT*TMSSPSC *KTKMILIKQLTF*IEAQA*KALGYCCCPRTETI TVPLPTLGCPDRCGSRLPSPQGI*ILSTSPPSP EAGTSLSAPRTLAEAHLPLAMFLSGSSTLPGRG PTPASTVRGSSSQRPASSACWIP*AVQKIPCLEA ANPWTGQQTAHPSGNHECPVPRASLTTDRNTQI GKLSFMTKGSKVEPTPGATTCLCTTRTTVMAEE HFPEYGVIKATCSPWCPPAAP*AQMARTWVWLCN TWTPVGACGVRTARMPSLCRRGMCQPSLPVPPS TGAGESSWARVPSAGSICAMTWTRDVNLLPSRS NLIQTVLRQARR*VLWSARSSC*RTCSMSASCST MAVCGTALRRP*PSSWSTCQGAR*KTS*RLTVL *QRA*PESTRGRSWRACPTCTAT*LFTGTLREP TSSETLLGM*SWGTLGPANACRRSVCRGRACAPS LAHPTG*ALR*SAARAMEGKQTCGAWAALWWRC *QRNHRGQSMKLWPPSSRLPPSPPILSCPPTSL NMAGTS*GAFLWRLARDLQLRSCSHTTLHSSCTE LSRPHSCRSPFAAWQGAAAGLSEVAASPRQGCG PWSGSPASVGLCPFRHWGSEPGWGGCSLRTGSP QPVRSRSSSVLSSAWRGRGWEQCARQPWAPPSGM CPDTAIGTEAQRVWGHKTDARV*RVLFSFKVLF CFSFQCLETTRASLGWMSSHKPEGKASTR*QWQ AEAQAAVP*SPRLALPVLSFTKDE*SKCHAALFR EGGACPACGHDPASPRQGPAMWNCCH*GGIQFC QCSCLCFTSWSHSYAVPSF*TGDCVCPLGSEYP CFGLGPRLH*KELKVVAFALLAQPLFPTGAEGEM DDTVGASGLASALIPESPRRCLRLPES*PARPE PTPSGRRESPYATTSCVQNRQLCSSSASLAHPL EVSAPFPCSSSPGGGMAAGVGETASPSSLELAIF TSAWALVLSSGPSPPKCAYC*SSSLSTPSFLGS CH*RKKKKKKASAQGWASPGSWGLVPGSPASHA YIPMGTEQAKAFVVC*RCTFMNVVSIAVFSLFTS CNVRPCTC*FISQIIFDGFYIYQF*TVTGDGRL KREKRK*KQLVLQKCVSHAPVGPGPSCVHPCSP RGSISPCTPHCPLKTTQAPASTSALAQGGAWPSS *PKLLCGSSASLRPHLGGCTLFLARTPIPSPCS PRGYRARCWLTQLCVPGSGSYRAPPPGVLPHWE CVLKMNLKTSQQTLHSKKAKQTLIFLCQKLWTCW LSILRTKLLLNLLFFNN*SR*KVVGLQGDLGPK VLKGSSWQPQACCGKGPCRHFLIIPWGVSAWAN SAWRGQGWGQSALCHPPAPSTHPSSMSVSELWIV QPWLLWNCGTCSHSYLTIS*PRACSAKPGQCCA LLTIKGIIYIR

Sequence NM_203351

GAGAACGGTGGCCGGACGGAGAGACTGCGGGTCTGAGGGACTGGCGGGCGGGCGGGCCGAGCGGCGCCGCCGAGGCCGGGCTGGGCCGAGCCCAGGAGCG CCCGGGATGTAGCGGGCCACCCTGCCGATGCCACAGCGCCCGGCCGCGGGCGGAGCCGGAGCCGGAGCCTGGGGAGGCGGCGGGGGCCCAGAGCGCAGCC CGCGCCCCCCGCGCGGAGCCAGGCCCGCTGCCGTCCCCGCCGCCCGGGCCCCCGGCATGCAGCCCCGGCTGCGGAGGTGACACTCACGGACCTTAGCCAC CGCCGCCGCCATCGCCACCATGGACGAACAGGAGGCATTGAACTCAATCATGAACGATCTGGTGGCCCTCCAGATGAACCGACGTCACCGGATGCCTGGA TATGAGACCATGAAGAACAAAGACACAGGTCACTCAAATAGGCAGAAAAAACACAACAGCAGCAGCTCAGCCCTTCTGAACAGCCCCACAGTAACAACAA GCTCATGTGCAGGGGCCAGTGAGAAAAAGAAATTTTTGAGTGACGTCAGAATCAAGTTCGAGCACAACGGGGAGAGGCGAATTATAGCGTTCAGCCGGCC TGTGAAATATGAAGATGTGGAGCACAAGGTGACAACAGTATTTGGACAACCTCTTGATCTACATTACATGAACAATGAGCTCTCCATCCTGCTGAAAAAC CAAGATGATCTTGATAAAGCAATTGACATTTTAGATAGAAGCTCAAGCATGAAAAGCCTTAGGATATTGCTGTTGTCCCAGGACAGAAACCATAACAGTT CCTCTCCCCACTCTGGGGTGTCCAGACAGGTGCGGATCAAGGCTTCCCAGTCCGCAGGGGATATAAATACTATCTACCAGCCCCCCGAGCCCAGAAGCAG GCACCTCTCTGTCAGCTCCCAGAACCCTGGCCGAAGCTCACCTCCCCCTGGCTATGTTCCTGAGCGGCAGCAGCACATTGCCCGGCAGGGGTCCTACACC AGCATCAACAGTGAGGGGGAGTTCATCCCAGAGACCAGCGAGCAGTGCATGCTGGATCCCCTGAGCAGTGCAGAAAATTCCTTGTCTGGAAGCTGCCAAT CCTTGGACAGGTCAGCAGACAGCCCATCCTTCCGGAAATCACGAATGTCCCGTGCCCAGAGCTTCCCTGACAACAGACAGGAATACTCAGATCGGGAAAC TCAGCTTTATGACAAAGGGGTCAAAGGTGGAACCTACCCCCGGCGCTACCACGTGTCTGTGCACCACAAGGACTACAGTGATGGCAGAAGAACATTTCCC CGAATACGGCGTCATCAAGGCAACTTGTTCACCCTGGTGCCCTCCAGCCGCTCCCTGAGCACAAATGGCGAGAACATGGGTCTGGCTGTGCAATACCTGG ACCCCCGTGGGCGCCTGCGGAGTGCGGACAGCGAGAATGCCCTCTCTGTGCAGGAGAGGAATGTGCCAACCAAGTCTCCCAGTGCCCCCATCAACTGGCG CCGGGGAAAGCTCCTGGGCCAGGGTGCCTTCGGCAGGGTCTATTTGTGCTATGACGTGGACACGGGACGTGAACTTGCTTCCAAGCAGGTCCAATTTGAT CCAGACAGTCCTGAGACAAGCAAGGAGGTGAGTGCTCTGGAGTGCGAGATCCAGTTGCTAAAGAACTTGCAGCATGAGCGCATCGTGCAGTACTATGGCT GTCTGCGGGACCGCGCTGAGAAGACCCTGACCATCTTCATGGAGTACATGCCAGGGGGCTCGGTGAAAGACCAGTTGAAGGCTTACGGTGCTCTGACAGA GAGCGTGACCCGAAAGTACACGCGGCAGATCCTGGAGGGCATGTCCTACCTGCACAGCAACATGATTGTTCACCGGGACATTAAGGGAGCCAACATCCTC CGAGACTCTGCTGGGAATGTAAAGCTGGGGGACTTTGGGGCCAGCAAACGCCTGCAGACGATCTGTATGTCGGGGACGGGCATGCGCTCCGTCACTGGCA CACCCTACTGGATGAGCCCTGAGGTGATCAGCGGCGAGGGCTATGGAAGGAAAGCAGACGTGTGGAGCCTGGGCTGCACTGTGGTGGAGATGCTGACAGA GAAACCACCGTGGGCAGAGTATGAAGCTATGGCCGCCATCTTCAAGATTGCCACCCAGCCCACCAATCCTCAGCTGCCCTCCCACATCTCTGAACATGGC CGGGACTTCCTGAGGCGCATTTTTGTGGAGGCTCGCCAGAGACCTTCAGCTGAGGAGCTGCTCACACACCACTTTGCACAGCTCATGTACTGAGCTCTCA CGGCCACACAGCTGCCGGTCGCCCTTTGCTGCATGGCAGGGGGCTGCTGCTGGGCTCAGTGAAGTTGCTGCTTCTCCCAGGCAAGGCTGTGGACCATGGA GTGGCAGCCCAGCCAGCGTCGGTCTGTGCCCCTTCCGCCACTGGGGCTCAGAGCCGGGGTGGGGTGGCTGCAGCCTCAGGACTGGGAGCCCCCAGCCTGT CAGATCCAGGAGCTCCAGTGTCCTGAGCTCAGCGTGGAGGGGTAGGGGCTGGGAACAGTGTGCAAGGCAGCCGTGGGCCCCACCCTCGGGGATGTGTCCT GACACTGCAATTGGCACCGAAGCCCAGAGGGTCTGGGGGCACAAGACTGACGCCAGGGTATGAAGAGTGTTATTTTCATTCAAAGTGTTATTTTGTTTTT CCTTCCAATGTCTGGAGACCACCAGGGCATCTCTGGGCTGGATGAGCTCCCACAAGCCTGAGGGAAAGGCCAGCACTCGCTAGCAGTGGCAGGCAGAGGC CCAGGCTGCCGTCCCCTAGAGTCCCAGGTTGGCTCTGCCAGTCCTGTCCTTTACCAAAGATGAATGAAGCAAATGTCATGCTGCCTTATTCAGGGAAGGA GGAGCCTGTCCTGCCTGTGGCCATGACCCTGCCTCTCCCAGGCAGGGGCCCGCGATGTGGAACTGCTGCCACTGAGGGGGGATCCAGTTTTGTCAATGCA GTTGTCTCTGTTTTACAAGTTGGAGTCACTCTTATGCTGTACCCAGTTTCTAAACTGGAGACTGTGTGTGCCCTCTGGGCTCTGAGTACCCCTGCTTTGG GCTTGGGCCTAGGCTGCATTGAAAAGAGCTGAAGGTTGTGGCCTTTGCGCTCCTGGCCCAGCCTTTGTTCCCCACTGGAGCAGAAGGGGAGATGGACGAC ACGGTCGGGGCATCTGGCCTGGCCAGTGCCCTGATCCCAGAGAGCCCGAGGAGGTGTCTCAGGCTGCCTGAGTCGTGACCTGCTAGGCCAGAGCCCACTC CATCTGGTAGAAGGGAAAGCCCATATGCTACCACCAGCTGTGTCCAAAACCGCCAGCTCTGTTCTTCCTCAGCCAGCCTCGCCCATCCCCTTGAGGTCTC AGCCCCTTTCCCTTGTAGCTCCTCCCCTGGAGGGGGAATGGCAGCAGGGGTTGGGGAAACAGCATCTCCAAGCAGCTTAGAGTTGGCCATATTTACCTCA GCCTGGGCGCTGGTCCTTTCTTCCGGCCCCTCCCCTCCAAAATGTGCCTATTGCTAGAGCTCCTCCCTCTCAACACCCAGTTTCCTTGGGAGTTGTCATT AAAGGAAAAAAAAAAAAAAAAAAGCCAGTGCCCAGGGATGGGCATCTCCAGGGAGCTGGGGATTAGTGCCAGGCAGCCCTGCCAGCCATGCCTACATCCC CATGGGCACAGAACAAGCCAAAGCCTTCGTTGTATGTTGACGATGCACTTTTATGAATGTAGTTTCTATCGCTGTTTTTAGCCTTTTCACATCATGTAAT GTGAGGCCTTGTACTTGTTAATTTATATCTCAGATCATATTTGATGGTTTTTATATATATCAATTCTAGACTGTTACAGGTGACGGACGCCTCAAGAGAG AGAAGAGAAAATGAAAGCAGCTGGTTTTGCAGAAGTGTGTGTCGCATGCGCCAGTTGGGCCTGGACCCTCCTGTGTCCATCCCTGTTCCCCCAGGGGCTC TATCAGCCCCTGTACCCCACACTGCCCTCTGAAGACAACACAGGCTCCTGCTTCCACCTCGGCCCTTGCCCAGGGTGGGGCCTGGCCCTCATCTTGACCA AAGCTGCTGTGTGGCAGCTCGGCCTCTCTACGACCCCATCTTGGTGGCTGCACACTCTTCCTGGCCCGCACCCCCATCCCCAGTCCCTGTTCCCCAAGAG GATACAGAGCACGGTGCTGGCTGACTCAACTGTGCGTCCCAGGTTCAGGGTCTTACAGAGCTCCACCCCCTGGGGTCTTACCTCACTGGGAATGTGTTTT GAAAATGAATTTGAAGACAAGCCAACAAACCCTGCACTCCAAAAAAGCAAAACAGACCCTAATTTTTTTGTGCCAAAAACTGTGGACATGCTGGCTCAGC ATCCTCAGGACCAAGTTGTTGCTTAATTTATTGTTTTTTAATAACTAATCCAGATAAAAAGTTGTGGGGCTTCAGGGTGACCTGGGCCCAAAGGTTCTGA AGGGCAGTTCCTGGCAGCCCCAGGCTTGCTGTGGGAAGGGGCCGTGCCGTCACTTTCTCATCATTCCATGGGGTGTGTCTGCCTGGGCCAACTCTGCATG GAGAGGCCAGGGCTGGGGACAGTCCGCACTCTGCCACCCTCCTGCCCCTTCCACCCACCCCAGCTCTATGTCTGTGTCTGAATTGTGGATCGTGCAGCCA TGGTTATTGTGGAACTGTGGAACCTGCAGCCATAGTTATTTGACTATATCTTGACCGAGGGCTTGCAGTGCAAAGCCAGGCCAGTGTTGCGCATTACTTA CAATAAAAGGGATCATTTATATCAGA

refseq_MAP3K3.F2 (forward primer)
refseq_MAP3K3.R2 (reverse primer)
Single exon skipping

Frame 0

ENGGRTERLRV*GTGGRAGRAAPPRPGWAEPRS ARDVAGHPADATAPGRGRSRSRSLGRRRGPRAQP APPARSQARCRPRRPGPRHAAPAAEVTLTDLSH RRRHRHHGRTGGIELNHERSGGPPDEPTSPDAW I*DHEEQRHRSLK*AEKTQQQQLSPSEQPHSNNK LMCRGQ*EKEIFE*RQNQVRAQRGEANYSVQPA CEI*RCGAQGDNSIWTTS*STLHEQ*ALHPAEK PR*S**SN*HFR*KLKHEKP*DIAVVPGQKP*QF LSPLWGVQTGADQGFPVRRGYKYYLPAPRAQKQ APLCQLPEPWPKLTSPWLCS*AAAAHCPAGVLH QHQQ*GGVHPRDQRAVHAGSPEQCRKFLVWKLPI LGQVSRQPILPEITNVPCPELP*QQTGILRSGN SAL*QRGQRWNLPPALPRVCAPQGLQ*WQKNIS PNTASSRQLVHPGALQPLPEHKWREHGSGCAIPG PPWAPAECGQRECPLCAGEECANQVSQCPHQLA PGKAPGPGCLRQGLFVL*RGHGT*TCFQAGPI* SRQS*DKQGGECSGVRDPVAKELAA*AHRAVLWL SAGPR*EDPDHLHGVHARGLGERPVEGLRCSDR ERDPKVHAADPGGHVLPAQQHDCSPGH*GSQHP PRLCWECKAGGLWGQQTPADDLYVGDGHALRHWH TLLDEP*GDQRRGLWKESRRVEPGLHCGGDADR ETTVGRV*SYGRHLQDCHPAHQSSAALPHL*TW PGLPEAHFCGGSPETFS*GAAHTPLCTAHVLSSH GHTAAGRPLLHGRGLLLGSVKLLLLPGKAVDHG VAAQPASVCAPSATGAQSRGGVAAASGLGAPSL SDPGAPVS*AQRGGVGAGNSVQGSRGPHPRGCVL TLQLAPKPRGSGGTRLTPGYEECYFHSKCYFVF PSNVWRPPGHLWAG*APTSLRERPALASSGRQR PRLPSPRVPGWLCQSCPLPKMNEANVMLPYSGKE EPVLPVAMTLPLPGRGPRCGTAATEGGSSFVNA VVSVLQVGVTLMLYPVSKLETVCALWALSTPAL GLGLGCIEKS*RLWPLRSWPSLCSPLEQKGRWTT RSGHLAWPVP*SQRARGGVSGCLSRDLLGQSPL HLVEGKAHMLPPAVSKTASSVLPQPASPIPLRS QPLSLVAPPLEGEWQQGLGKQHLQAA*SWPYLPQ PGRWSFLPAPPLQNVPIARAPPSQHPVSLGVVI KGKKKKKKPVPRDGHLQGAGD*CQAALPAMPTS PWAQNKPKPSLYVDDALL*M*FLSLFLAFSHHVM *GLVLVNLYLRSYLMVFIYINSRLLQVTDASRE RRENESSWFCRSVCRMRQLGLDPPVSIPVPPGA LSAPVPHTAL*RQHRLLLPPRPLPRVGPGPHLDQ SCCVAARPLYDPILVAAHSSWPAPPSPVPVPQE DTEHGAG*LNCASQVQGLTELHPLGSYLTGNVF *K*I*RQANKPCTPKKQNRP*FFCAKNCGHAGSA SSGPSCCLIYCFLITNPDKKLWGFRVTWAQRF* RAVPGSPRLAVGRGRAVTFSSFHGVCLPGPTLH GEARAGDSPHSATLLPLPPTPALCLCLNCGSCSH GYCGTVEPAAIVI*LYLDRGLAVQSQASVAHYL Q*KGSFIS

Frame 1

RTVAGRRDCGSEGLAGGRAERRRRGRAGPSPGA PGM*RATLPMPQRPAAGGAGAGAWGGGGGPERS PRPPRGARPAAVPAARAPGMQPRLRR*HSRTLAT AAAIATMDEQEALNSIMNDLVALQMNRRHRMPG YETMKNKDTGHSNRQKKHNSSSSALLNSPTVTT SSCAGASEKKKFLSDVRIKFEHNGERRIIAFSRP VKYEDVEHKVTTVFGQPLDLHYMNNELSILLKN QDDLDKAIDILDRSSSMKSLRILLLSQDRNHNS SSPHSGVSRQVRIKASQSAGDINTIYQPPEPRSR HLSVSSQNPGRSSPPPGYVPERQQHIARQGSYT SINSEGEFIPETSEQCMLDPLSSAENSLSGSCQ SLDRSADSPSFRKSRMSRAQSFPDNRQEYSDRET QLYDKGVKGGTYPRRYHVSVHHKDYSDGRRTFP RIRRHQGNLFTLVPSSRSLSTNGENMGLAVQYL DPRGRLRSADSENALSVQERNVPTKSPSAPINWR RGKLLGQGAFGRVYLCYDVDTGRELASKQVQFD PDSPETSKEVSALECEIQLLKNLQHERIVQYYG CLRDRAEKTLTIFMEYMPGGSVKDQLKAYGALTE SVTRKYTRQILEGMSYLHSNMIVHRDIKGANIL RDSAGNVKLGDFGASKRLQTICMSGTGMRSVTG TPYWMSPEVISGEGYGRKADVWSLGCTVVEMLTE KPPWAEYEAMAAIFKIATQPTNPQLPSHISEHG RDFLRRIFVEARQRPSAEELLTHHFAQLMY*AL TATQLPVALCCMAGGCCWAQ*SCCFSQARLWTME WQPSQRRSVPLPPLGLRAGVGWLQPQDWEPPAC QIQELQCPELSVEG*GLGTVCKAAVGPTLGDVS *HCNWHRSPEGLGAQD*RQGMKSVIFIQSVILFF LPMSGDHQGISGLDELPQA*GKGQHSLAVAGRG PGCRPLESQVGSASPVLYQR*MKQMSCCLIQGR RSLSCLWP*PCLSQAGARDVELLPLRGDPVLSMQ LSLFYKLESLLCCTQFLNWRLCVPSGL*VPLLW AWA*AALKRAEGCGLCAPGPAFVPHWSRRGDGR HGRGIWPGQCPDPREPEEVSQAA*VVTC*ARAHS IW*KGKPICYHQLCPKPPALFFLSQPRPSP*GL SPFPL*LLPWRGNGSRGWGNSISKQLRVGHIYL SLGAGPFFRPLPSKMCLLLELLPLNTQFPWELSL KEKKKKKSQCPGMGISRELGISARQPCQPCLHP HGHRTSQSLRCMLTMHFYECSFYRCF*PFHIM* CEALYLLIYISDHI*WFLYISILDCYR*RTPQER EEKMKAAGFAEVCVACASWAWTLLCPSLFPQGL YQPLYPTLPSEDNTGSCFHLGPCPGWGLALILT KAAVWQLGLSTTPSWWLHTLPGPHPHPQSLFPKR IQSTVLADSTVRPRFRVLQSSTPWGLTSLGMCF ENEFEDKPTNPALQKSKTDPNFFVPKTVDMLAQ HPQDQVVA*FIVF**LIQIKSCGASG*PGPKGSE GQFLAAPGLLWEGAVPSLSHHSMGCVCLGQLCM ERPGLGTVRTLPPSCPFHPPQLYVCV*IVDRAA MVIVELWNLQP*LFDYILTEGLQCKARPVLRITY NKRDHLYQ

Frame 2

ERWPDGETAGLRDWRAGGPSGAAEAGLGRAQER PGCSGPPCRCHSARPRAEPEPEPGEAAGAQSAA RAPRAEPGPLPSPPPGPPACSPGCGGDTHGP*P PPPPSPPWTNRRH*TQS*TIWWPSR*TDVTGCLD MRP*RTKTQVTQIGRKNTTAAAQPF*TAPQ*QQ AHVQGPVRKRNF*VTSESSSSTTGRGEL*RSAG L*NMKMWSTR*QQYLDNLLIYIT*TMSSPSC*KT KMILIKQLTF*IEAQA*KALGYCCCPRTETITV PLPTLGCPDRCGSRLPSPQGI*ILSTSPPSPEA GTSLSAPRTLAEAHLPLAMFLSGSSTLPGRGPTP ASTVRGSSSQRPASSACWIP*AVQKIPCLEAAN PWTGQQTAHPSGNHECPVPRASLTTDRNTQIGK LSFMTKGSKVEPTPGATTCLCTTRTTVMAEEHFP EYGVIKATCSPWCPPAAP*AQMARTWVWLCNTW TPVGACGVRTARMPSLCRRGMCQPSLPVPPSTG AGESSWARVPSAGSICAMTWTRDVNLLPSRSNLI QTVLRQARR*VLWSARSSC*RTCSMSASCSTMA VCGTALRRP*PSSWSTCQGAR*KTS*RLTVL*Q RA*PESTRGRSWRACPTCTAT*LFTGTLREPTSS ETLLGM*SWGTLGPANACRRSVCRGRACAPSLA HPTG*ALR*SAARAMEGKQTCGAWAALWWRC*Q RNHRGQSMKLWPPSSRLPPSPPILSCPPTSLNMA GTS*GAFLWRLARDLQLRSCSHTTLHSSCTELS RPHSCRSPFAAWQGAAAGLSEVAASPRQGCGPW SGSPASVGLCPFRHWGSEPGWGGCSLRTGSPQPV RSRSSSVLSSAWRGRGWEQCARQPWAPPSGMCP DTAIGTEAQRVWGHKTDARV*RVLFSFKVLFCF SFQCLETTRASLGWMSSHKPEGKASTR*QWQAEA QAAVP*SPRLALPVLSFTKDE*SKCHAALFREG GACPACGHDPASPRQGPAMWNCCH*GGIQFCQC SCLCFTSWSHSYAVPSF*TGDCVCPLGSEYPCFG LGPRLH*KELKVVAFALLAQPLFPTGAEGEMDD TVGASGLASALIPESPRRCLRLPES*PARPEPT PSGRRESPYATTSCVQNRQLCSSSASLAHPLEVS APFPCSSSPGGGMAAGVGETASPSSLELAIFTS AWALVLSSGPSPPKCAYC*SSSLSTPSFLGSCH *RKKKKKKASAQGWASPGSWGLVPGSPASHAYIP MGTEQAKAFVVC*RCTFMNVVSIAVFSLFTSCN VRPCTC*FISQIIFDGFYIYQF*TVTGDGRLKR EKRK*KQLVLQKCVSHAPVGPGPSCVHPCSPRGS ISPCTPHCPLKTTQAPASTSALAQGGAWPSS*P KLLCGSSASLRPHLGGCTLFLARTPIPSPCSPR GYRARCWLTQLCVPGSGSYRAPPPGVLPHWECVL KMNLKTSQQTLHSKKAKQTLIFLCQKLWTCWLS ILRTKLLLNLLFFNN*SR*KVVGLQGDLGPKVL KGSSWQPQACCGKGPCRHFLIIPWGVSAWANSAW RGQGWGQSALCHPPAPSTHPSSMSVSELWIVQP WLLWNCGTCSHSYLTIS*PRACSAKPGQCCALL TIKGIIYIR