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Sequence NM_005606
GCACAGTGGCCCTTAAGCGAGGAGCGGCGGCGCCCGCAGCAATCACAGCAGTGCCGACGTCGTGGGTGTTTGGTGTGAGGCTGCGAGCCGCCGCGAGTTCTCACGGTCCCGCCGGCGCCACCACCGCGGTCACTCACCGCCGCCGCCGCCACCACTGCCACCACGGTCGCCTGCCACAGGTGTCTGCAATTGAACTCCAA
GGTGCAGAATGGTTTGGAAAGTAGCTGTATTCCTCAGTGTGGCCCTGGGCATTGGTGCCGTTCCTATAGATGATCCTGAAGATGGAGGCAAGCACTGGGT
GGTGATCGTGGCAGGTTCAAATGGCTGGTATAATTATAGGCACCAGGCAGACGCGTGCCATGCCTACCAGATCATTCACCGCAATGGGATTCCTGACGAA
CAGATCGTTGTGATGATGTACGATGACATTGCTTACTCTGAAGACAATCCCACTCCAGGAATTGTGATCAACAGGCCCAATGGCACAGATGTCTATCAGG
GAGTCCCGAAGGACTACACTGGAGAGGATGTTACCCCACAAAATTTCCTTGCTGTGTTGAGAGGCGATGCAGAAGCAGTGAAGGGCATAGGATCCGGCAA
AGTCCTGAAGAGTGGCCCCCAGGATCACGTGTTCATTTACTTCACTGACCATGGATCTACTGGAATACTGGTTTTTCCCAATGAAGATCTTCATGTAAAG
GACCTGAATGAGACCATCCATTACATGTACAAACACAAAATGTACCGAAAGATGGTGTTCTACATTGAAGCCTGTGAGTCTGGGTCCATGATGAACCACC
TGCCGGATAACATCAATGTTTATGCAACTACTGCTGCCAACCCCAGAGAGTCGTCCTACGCCTGTTACTATGATGAGAAGAGGTCCACGTACCTGGGGGA
CTGGTACAGCGTCAACTGGATGGAAGATTCGGACGTGGAAGATCTGACTAAAGAGACCCTGCACAAGCAGTACCACCTGGTAAAATCGCACACCAACACC
AGCCACGTCATGCAGTATGGAAACAAAACAATCTCCACCATGAAAGTGATGCAGTTTCAGGGTATGAAACGCAAAGCCAGTTCTCCCGTCCCCCTACCTC
CAGTCACACACCTTGACCTCACCCCCAGCCCTGATGTGCCTCTCACCATCATGAAAAGGAAACTGATGAACACCAATGATCTGGAGGAGTCCAGGCAGCT
CACGGAGGAGATCCAGCGGCATCTGGATGCCAGGCACCTCATTGAGAAGTCAGTGCGTAAGATCGTCTCCTTGCTGGCAGCGTCCGAGGCTGAGGTGGAG
CAGCTCCTGTCCGAGAGAGCCCCGCTCACGGGGCACAGCTGCTACCCAGAGGCCCTGCTGCACTTCCGGACCCACTGCTTCAACTGGCACTCCCCCACGT
ACGAGTATGCGTTGAGACATTTGTACGTGCTGGTCAACCTTTGTGAGAAGCCGTATCCGCTTCACAGGATAAAATTGTCCATGGACCACGTGTGCCTTGG
TCACTACTGAAGAGCTGCCTCCTGGAAGCTTTTCCAAGTGTGAGCGCCCCACCGACTGTGTGCTGATCAGAGACTGGAGAGGTGGAGTGAGAAGTCTCCG
CTGCTCGGGCCCTCCTGGGGAGCCCCCGCTCCAGGGCTCGCTCCAGGACCTTCTTCACAAGATGACTTGCTCGCTGTTACCTGCTTCCCCAGTCTTTTCT
GAAAAACTACAAATTAGGGTGGGAAAAGCTCTGTATTGAGAAGGGTCATATTTGCTTTCTAGGAGGTTTGTTGTTTTGCCTGTTAGTTTTGAGGAGCAGG
AAGCTCATGGGGGCTTCTGTAGCCCCTCTCAAAAGGAGTCTTTATTCTGAGAATTTGAAGCTGAAACCTCTTTAAATCTTCAGAATGATTTTATTGAAGA
GGGCCGCAAGCCCCAAATGGAAAACTGTTTTTAGAAAATATGATGATTTTTGATTGCTTTTGTATTTAATTCTGCAGGTGTTCAAGTCTTAAAAAATAAA
GATTTATAACAGAACCCAAA
- refseq_LGMN.F2 (forward primer)
- refseq_LGMN.R2 (reverse primer)
- Single exon skipping
Frame 0
AQWPLSEERRRPQQSQQCRRRGCLV*GCEPPRVLTVPPAPPPRSLTAAAATTATTVACHRCLQLNSK
VQNGLESSCIPQCGPGHWCRSYR*S*RWRQALG
GDRGRFKWLV*L*APGRRVPCLPDHSPQWDS*R
TDRCDDVR*HCLL*RQSHSRNCDQQAQWHRCLSG
SPEGLHWRGCYPTKFPCCVERRCRSSEGHRIRQ
SPEEWPPGSRVHLLH*PWIYWNTGFSQ*RSSCK
GPE*DHPLHVQTQNVPKDGVLH*SL*VWVHDEPP
AG*HQCLCNYCCQPQRVVLRLLL**EEVHVPGG
LVQRQLDGRFGRGRSD*RDPAQAVPPGKIAHQH
QPRHAVWKQNNLHHESDAVSGYETQSQFSRPPTS
SHTP*PHPQP*CASHHHEKETDEHQ*SGGVQAA
HGGDPAASGCQAPH*EVSA*DRLLAGSVRG*GG
AAPVRESPAHGAQLLPRGPAALPDPLLQLALPHV
RVCVETFVRAGQPL*EAVSASQDKIVHGPRVPW
SLLKSCLLEAFPSVSAPPTVC*SETGEVE*EVS
AARALLGSPRSRARSRTFFTR*LARCYLLPQSFL
KNYKLGWEKLCIEKGHICFLGGLLFCLLVLRSR
KLMGASVAPLKRSLYSENLKLKPL*IFRMILLK
RAASPKWKTVFRKYDDF*LLLYLILQVFKS*KIK
IYNRTQ
Frame 1
HSGP*ARSGGARSNHSSADVVGVWCEAASRREFSRSRRRHHRGHSPPPPPPLPPRSPATGVCN*TP
RCRMVWKVAVFLSVALGIGAVPIDDPEDGGKHWV
VIVAGSNGWYNYRHQADACHAYQIIHRNGIPDE
QIVVMMYDDIAYSEDNPTPGIVINRPNGTDVYQ
GVPKDYTGEDVTPQNFLAVLRGDAEAVKGIGSGK
VLKSGPQDHVFIYFTDHGSTGILVFPNEDLHVK
DLNETIHYMYKHKMYRKMVFYIEACESGSMMNH
LPDNINVYATTAANPRESSYACYYDEKRSTYLGD
WYSVNWMEDSDVEDLTKETLHKQYHLVKSHTNT
SHVMQYGNKTISTMKVMQFQGMKRKASSPVPLP
PVTHLDLTPSPDVPLTIMKRKLMNTNDLEESRQL
TEEIQRHLDARHLIEKSVRKIVSLLAASEAEVE
QLLSERAPLTGHSCYPEALLHFRTHCFNWHSPT
YEYALRHLYVLVNLCEKPYPLHRIKLSMDHVCLG
HY*RAASWKLFQV*APHRLCADQRLERWSEKSP
LLGPSWGAPAPGLAPGPSSQDDLLAVTCFPSLF
*KTTN*GGKSSVLRRVIFAF*EVCCFAC*F*GAG
SSWGLL*PLSKGVFILRI*S*NLFKSSE*FY*R
GPQAPNGKLFLENMMIFDCFCI*FCRCSSLKK*
RFITEPK
Frame 2
TVALKRGAAAPAAITAVPTSWVFGVRLRAAASSHGPAGATTAVTHRRRRHHCHHGRLPQVSAIELQ
GAEWFGK*LYSSVWPWALVPFL*MILKMEASTG
W*SWQVQMAGIIIGTRQTRAMPTRSFTAMGFLTN
RSL**CTMTLLTLKTIPLQEL*STGPMAQMSIR
ESRRTTLERMLPHKISLLC*EAMQKQ*RA*DPA
KS*RVAPRITCSFTSLTMDLLEYWFFPMKIFM*R
T*MRPSITCTNTKCTERWCSTLKPVSLGP**TT
CRITSMFMQLLLPTPESRPTPVTMMRRGPRTWG
TGTASTGWKIRTWKI*LKRPCTSSTTW*NRTPTP
ATSCSMETKQSPP*K*CSFRV*NAKPVLPSPYL
QSHTLTSPPALMCLSPS*KGN**TPMIWRSPGS
SRRRSSGIWMPGTSLRSQCVRSSPCWQRPRLRWS
SSCPREPRSRGTAATQRPCCTSGPTASTGTPPR
TSMR*DICTCWSTFVRSRIRFTG*NCPWTTCAL
VTTEELPPGSFSKCERPTDCVLIRDWRGGVRSLR
CSGPPGEPPLQGSLQDLLHKMTCSLLPASPVFS
EKLQIRVGKALY*EGSYLLSRRFVVLPVSFEEQ
EAHGGFCSPSQKESLF*EFEAETSLNLQNDFIEE
GRKPQMENCF*KI**FLIAFVFNSAGVQVLKNK
DL*QNP
Sequence NM_001008530
GCACAGTGGCCCTTAAGCGAGGAGCGGCGGCGCCCGCAGCAATCACAGCAGTGCCGACGTCGTGGGTGTTTGGTGTGAGGCTGCGAGCCGCCGCGAGTTCTCACGGTCCCGCCGGCGCCACCACCGCGGTCACTCACCGCCGCCGCCGCCACCACTGCCACCACGGTCGCCTGCCACAGGGTCTTACTCTGTTGCCCAGG
CTGGAGTGCAGTGGCACAATCTTGGCTCACTGCAACCTCTGCCTCCCGGGTTCAAGCAATTCTCCTGCCTCAGCCTCCCGAGTAGCTGGGATTACAGGTG
TCTGCAATTGAACTCCAAGGTGCAGAATGGTTTGGAAAGTAGCTGTATTCCTCAGTGTGGCCCTGGGCATTGGTGCCGTTCCTATAGATGATCCTGAAGA
TGGAGGCAAGCACTGGGTGGTGATCGTGGCAGGTTCAAATGGCTGGTATAATTATAGGCACCAGGCAGACGCGTGCCATGCCTACCAGATCATTCACCGC
AATGGGATTCCTGACGAACAGATCGTTGTGATGATGTACGATGACATTGCTTACTCTGAAGACAATCCCACTCCAGGAATTGTGATCAACAGGCCCAATG
GCACAGATGTCTATCAGGGAGTCCCGAAGGACTACACTGGAGAGGATGTTACCCCACAAAATTTCCTTGCTGTGTTGAGAGGCGATGCAGAAGCAGTGAA
GGGCATAGGATCCGGCAAAGTCCTGAAGAGTGGCCCCCAGGATCACGTGTTCATTTACTTCACTGACCATGGATCTACTGGAATACTGGTTTTTCCCAAT
GAAGATCTTCATGTAAAGGACCTGAATGAGACCATCCATTACATGTACAAACACAAAATGTACCGAAAGATGGTGTTCTACATTGAAGCCTGTGAGTCTG
GGTCCATGATGAACCACCTGCCGGATAACATCAATGTTTATGCAACTACTGCTGCCAACCCCAGAGAGTCGTCCTACGCCTGTTACTATGATGAGAAGAG
GTCCACGTACCTGGGGGACTGGTACAGCGTCAACTGGATGGAAGATTCGGACGTGGAAGATCTGACTAAAGAGACCCTGCACAAGCAGTACCACCTGGTA
AAATCGCACACCAACACCAGCCACGTCATGCAGTATGGAAACAAAACAATCTCCACCATGAAAGTGATGCAGTTTCAGGGTATGAAACGCAAAGCCAGTT
CTCCCGTCCCCCTACCTCCAGTCACACACCTTGACCTCACCCCCAGCCCTGATGTGCCTCTCACCATCATGAAAAGGAAACTGATGAACACCAATGATCT
GGAGGAGTCCAGGCAGCTCACGGAGGAGATCCAGCGGCATCTGGATGCCAGGCACCTCATTGAGAAGTCAGTGCGTAAGATCGTCTCCTTGCTGGCAGCG
TCCGAGGCTGAGGTGGAGCAGCTCCTGTCCGAGAGAGCCCCGCTCACGGGGCACAGCTGCTACCCAGAGGCCCTGCTGCACTTCCGGACCCACTGCTTCA
ACTGGCACTCCCCCACGTACGAGTATGCGTTGAGACATTTGTACGTGCTGGTCAACCTTTGTGAGAAGCCGTATCCGCTTCACAGGATAAAATTGTCCAT
GGACCACGTGTGCCTTGGTCACTACTGAAGAGCTGCCTCCTGGAAGCTTTTCCAAGTGTGAGCGCCCCACCGACTGTGTGCTGATCAGAGACTGGAGAGG
TGGAGTGAGAAGTCTCCGCTGCTCGGGCCCTCCTGGGGAGCCCCCGCTCCAGGGCTCGCTCCAGGACCTTCTTCACAAGATGACTTGCTCGCTGTTACCT
GCTTCCCCAGTCTTTTCTGAAAAACTACAAATTAGGGTGGGAAAAGCTCTGTATTGAGAAGGGTCATATTTGCTTTCTAGGAGGTTTGTTGTTTTGCCTG
TTAGTTTTGAGGAGCAGGAAGCTCATGGGGGCTTCTGTAGCCCCTCTCAAAAGGAGTCTTTATTCTGAGAATTTGAAGCTGAAACCTCTTTAAATCTTCA
GAATGATTTTATTGAAGAGGGCCGCAAGCCCCAAATGGAAAACTGTTTTTAGAAAATATGATGATTTTTGATTGCTTTTGTATTTAATTCTGCAGGTGTT
CAAGTCTTAAAAAATAAAGATTTATAACAGAACCCAAA
- refseq_LGMN.F2 (forward primer)
- refseq_LGMN.R2 (reverse primer)
- Single exon skipping
Frame 0
AQWPLSEERRRPQQSQQCRRRGCLV*GCEPPRVLTVPPAPPPRSLTAAAATTATTVACHRVLLCCPG
WSAVAQSWLTATSASRVQAILLPQPPE*LGLQV
SAIELQGAEWFGK*LYSSVWPWALVPFL*MILK
MEASTGW*SWQVQMAGIIIGTRQTRAMPTRSFTA
MGFLTNRSL**CTMTLLTLKTIPLQEL*STGPM
AQMSIRESRRTTLERMLPHKISLLC*EAMQKQ*
RA*DPAKS*RVAPRITCSFTSLTMDLLEYWFFPM
KIFM*RT*MRPSITCTNTKCTERWCSTLKPVSL
GP**TTCRITSMFMQLLLPTPESRPTPVTMMRR
GPRTWGTGTASTGWKIRTWKI*LKRPCTSSTTW*
NRTPTPATSCSMETKQSPP*K*CSFRV*NAKPV
LPSPYLQSHTLTSPPALMCLSPS*KGN**TPMI
WRSPGSSRRRSSGIWMPGTSLRSQCVRSSPCWQR
PRLRWSSSCPREPRSRGTAATQRPCCTSGPTAS
TGTPPRTSMR*DICTCWSTFVRSRIRFTG*NCP
WTTCALVTTEELPPGSFSKCERPTDCVLIRDWRG
GVRSLRCSGPPGEPPLQGSLQDLLHKMTCSLLP
ASPVFSEKLQIRVGKALY*EGSYLLSRRFVVLP
VSFEEQEAHGGFCSPSQKESLF*EFEAETSLNLQ
NDFIEEGRKPQMENCF*KI**FLIAFVFNSAGV
QVLKNKDL*QNP
Frame 1
HSGP*ARSGGARSNHSSADVVGVWCEAASRREFSRSRRRHHRGHSPPPPPPLPPRSPATGSYSVAQ
AGVQWHNLGSLQPLPPGFKQFSCLSLPSSWDYRC
LQLNSKVQNGLESSCIPQCGPGHWCRSYR*S*R
WRQALGGDRGRFKWLV*L*APGRRVPCLPDHSP
QWDS*RTDRCDDVR*HCLL*RQSHSRNCDQQAQW
HRCLSGSPEGLHWRGCYPTKFPCCVERRCRSSE
GHRIRQSPEEWPPGSRVHLLH*PWIYWNTGFSQ
*RSSCKGPE*DHPLHVQTQNVPKDGVLH*SL*VW
VHDEPPAG*HQCLCNYCCQPQRVVLRLLL**EE
VHVPGGLVQRQLDGRFGRGRSD*RDPAQAVPPG
KIAHQHQPRHAVWKQNNLHHESDAVSGYETQSQF
SRPPTSSHTP*PHPQP*CASHHHEKETDEHQ*S
GGVQAAHGGDPAASGCQAPH*EVSA*DRLLAGS
VRG*GGAAPVRESPAHGAQLLPRGPAALPDPLLQ
LALPHVRVCVETFVRAGQPL*EAVSASQDKIVH
GPRVPWSLLKSCLLEAFPSVSAPPTVC*SETGE
VE*EVSAARALLGSPRSRARSRTFFTR*LARCYL
LPQSFLKNYKLGWEKLCIEKGHICFLGGLLFCL
LVLRSRKLMGASVAPLKRSLYSENLKLKPL*IF
RMILLKRAASPKWKTVFRKYDDF*LLLYLILQVF
KS*KIKIYNRTQ
Frame 2
TVALKRGAAAPAAITAVPTSWVFGVRLRAAASSHGPAGATTAVTHRRRRHHCHHGRLPQGLTLLPR
LECSGTILAHCNLCLPGSSNSPASASRVAGITG
VCN*TPRCRMVWKVAVFLSVALGIGAVPIDDPED
GGKHWVVIVAGSNGWYNYRHQADACHAYQIIHR
NGIPDEQIVVMMYDDIAYSEDNPTPGIVINRPN
GTDVYQGVPKDYTGEDVTPQNFLAVLRGDAEAVK
GIGSGKVLKSGPQDHVFIYFTDHGSTGILVFPN
EDLHVKDLNETIHYMYKHKMYRKMVFYIEACES
GSMMNHLPDNINVYATTAANPRESSYACYYDEKR
STYLGDWYSVNWMEDSDVEDLTKETLHKQYHLV
KSHTNTSHVMQYGNKTISTMKVMQFQGMKRKAS
SPVPLPPVTHLDLTPSPDVPLTIMKRKLMNTNDL
EESRQLTEEIQRHLDARHLIEKSVRKIVSLLAA
SEAEVEQLLSERAPLTGHSCYPEALLHFRTHCF
NWHSPTYEYALRHLYVLVNLCEKPYPLHRIKLSM
DHVCLGHY*RAASWKLFQV*APHRLCADQRLER
WSEKSPLLGPSWGAPAPGLAPGPSSQDDLLAVT
CFPSLF*KTTN*GGKSSVLRRVIFAF*EVCCFAC
*F*GAGSSWGLL*PLSKGVFILRI*S*NLFKSS
E*FY*RGPQAPNGKLFLENMMIFDCFCI*FCRC
SSLKK*RFITEPK
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