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Sequence NM_213595
AGGCGCAAGCCGGCAAGATGGCGGCGGCTGGGGCTTTCCGTCTGAGGCGGGCGGCATCGGCTCTGCTGCTGCGGAGCCCCCGCCTGCCCGCCCGGAGCTGTCGGCCCCGGCCCGACTCTATCACAAGAAGGTTGTTGATCATTATGAAAATCCTAGAAACGTGGGGTCCCTTGACAAGACATCTAAAAATGTTGGAACTG
GACTGGTGGGGGCTCCAGCATGTGGTGACGTAATGAAATTACAGATTCAAGTGGATGAAAAGGGGAAGATTGTGGATGCTAGGTTTAAAACATTTGGCTG
TGGTTCCGCAATTGCCTCCAGCTCATTAGCCACTGAATGGGTGAAAGGAAAGACGGTGGAGGAAGCCTTGACTATCAAAAACACAGATATCGCCAAGGAG
CTCTGCCTTCCTCCCGTGAAACTGCACTGCTCCATGCTGGCTGAAGATGCAATCAAGGCCGCCCTGGCTGATTACAAATTGAAACAAGAACCCAAAAAAG
GAGAGGCAGAGAAGAAATGAGCCCTCCCTCGGCGAAGCCTCCAGCAGGCCACACCAGCTGTTTCCCACCTGCTGTGCAGTCACCTTAGATGTTCAGAAGC
CGCTTCCTCTCCACTGAAGAGCTATGAGATACGCACAATACTTGCTGTTCACGTTATGACTCTCATGCAAGCAAAATACACAGTTTCATTGTTCTGAATC
CTGTGGTTTCTTTCAGCCCACTTTTATCGCCTTAACCTAGTTAATGTATATTTTGAATTGTGTGTATGACCTCAGAACTGAAATTGATAATGAAGTTGCA
AGTTTTGATAGCCCGTGAAGTGCATAAGTATCTAATTTTACCTGAATTGATTTGGGGGGAAATTACCAGTAGAATGCCTTGGTCTGAATATTTGATAGAA
CCAATTGTTGTACATAAAACAGATTGCGCATATATATATATGTATAAAAAATAATAAAATAATGGAAGATGA
- refseq_NIFUN.F1 (forward primer)
- refseq_NIFUN.R1 (reverse primer)
- Single exon skipping
Frame 0
RRKPARWRRLGLSV*GGRHRLCCCGAPACPPGAVGPGPTLSQEGC*SL*KS*KRGVP*QDI*KCWNW
TGGGSSMW*RNEITDSSG*KGEDCGC*V*NIWL
WFRNCLQLISH*MGERKDGGGSLDYQKHRYRQG
ALPSSRETALLHAG*RCNQGRPG*LQIETRTQKR
RGREEMSPPSAKPPAGHTSCFPPAVQSP*MFRS
RFLSTEEL*DTHNTCCSRYDSHASKIHSFIVLN
PVVSFSPLLSP*PS*CIF*IVCMTSELKLIMKLQ
VLIAREVHKYLILPELIWGEITSRMPWSEYLIE
PIVVHKTDCAYIYMYKK**NNGR*
Frame 1
GASRQDGGGWGFPSEAGGIGSAAAEPPPARPELSAPARLYHKKVVDHYENPRNVGSLDKTSKNVGT
GLVGAPACGDVMKLQIQVDEKGKIVDARFKTFGC
GSAIASSSLATEWVKGKTVEEALTIKNTDIAKE
LCLPPVKLHCSMLAEDAIKAALADYKLKQEPKK
GEAEKK*ALPRRSLQQATPAVSHLLCSHLRCSEA
ASSPLKSYEIRTILAVHVMTLMQAKYTVSLF*I
LWFLSAHFYRLNLVNVYFELCV*PQN*N***SC
KF**PVKCISI*FYLN*FGGKLPVECLGLNI**N
QLLYIKQIAHIYICIKNNKIMED
Frame 2
AQAGKMAAAGAFRLRRAASALLLRSPRLPARSCRPRPDSITRRLLIIMKILETWGPLTRHLKMLEL
DWWGLQHVVT**NYRFKWMKRGRLWMLGLKHLA
VVPQLPPAH*PLNG*KERRWRKP*LSKTQISPRS
SAFLP*NCTAPCWLKMQSRPPWLITN*NKNPKK
ERQRRNEPSLGEASSRPHQLFPTCCAVTLDVQK
PLPLH*RAMRYAQYLLFTL*LSCKQNTQFHCSES
CGFFQPTFIALT*LMYILNCVYDLRTEIDNEVA
SFDSP*SA*VSNFT*IDLGGNYQ*NALV*IFDR
TNCCT*NRLRIYIYV*KIIK*WKM
Sequence NM_014301
AGGCGCAAGCCGGCAAGATGGCGGCGGCTGGGGCTTTCCGTCTGAGGCGGGCGGCATCGGCTCTGCTGCTGCGGAGCCCCCGCCTGCCCGCCCGGAGCTGTCGGCCCCGGCCCGACTCTATCACAAGAAGGTATCTCAAATCTGTGAAGTATTGTAGAGGAGACACAAAAGGAATTGGGGGTCACAAATGGTTCTCATTG
ACATGAGTGTAGACCTTTCTACTCAGGTTGTTGATCATTATGAAAATCCTAGAAACGTGGGGTCCCTTGACAAGACATCTAAAAATGTTGGAACTGGACT
GGTGGGGGCTCCAGCATGTGGTGACGTAATGAAATTACAGATTCAAGTGGATGAAAAGGGGAAGATTGTGGATGCTAGGTTTAAAACATTTGGCTGTGGT
TCCGCAATTGCCTCCAGCTCATTAGCCACTGAATGGGTGAAAGGAAAGACGGTGGAGGAAGCCTTGACTATCAAAAACACAGATATCGCCAAGGAGCTCT
GCCTTCCTCCCGTGAAACTGCACTGCTCCATGCTGGCTGAAGATGCAATCAAGGCCGCCCTGGCTGATTACAAATTGAAACAAGAACCCAAAAAAGGAGA
GGCAGAGAAGAAATGAGCCCTCCCTCGGCGAAGCCTCCAGCAGGCCACACCAGCTGTTTCCCACCTGCTGTGCAGTCACCTTAGATGTTCAGAAGCCGCT
TCCTCTCCACTGAAGAGCTATGAGATACGCACAATACTTGCTGTTCACGTTATGACTCTCATGCAAGCAAAATACACAGTTTCATTGTTCTGAATCCTGT
GGTTTCTTTCAGCCCACTTTTATCGCCTTAACCTAGTTAATGTATATTTTGAATTGTGTGTATGACCTCAGAACTGAAATTGATAATGAAGTTGCAAGTT
TTGATAGCCCGTGAAGTGCATAAGTATCTAATTTTACCTGAATTGATTTGGGGGGAAATTACCAGTAGAATGCCTTGGTCTGAATATTTGATAGAACCAA
TTGTTGTACATAAAACAGATTGCGCATATATATATATGTATAAAAAATAATAAAATAATGGAAGATGA
- refseq_NIFUN.F1 (forward primer)
- refseq_NIFUN.R1 (reverse primer)
- Single exon skipping
Frame 0
RRKPARWRRLGLSV*GGRHRLCCCGAPACPPGAVGPGPTLSQEGISNL*SIVEETQKELGVTNGSH*
HECRPFYSGC*SL*KS*KRGVP*QDI*KCWNWT
GGGSSMW*RNEITDSSG*KGEDCGC*V*NIWLW
FRNCLQLISH*MGERKDGGGSLDYQKHRYRQGAL
PSSRETALLHAG*RCNQGRPG*LQIETRTQKRR
GREEMSPPSAKPPAGHTSCFPPAVQSP*MFRSR
FLSTEEL*DTHNTCCSRYDSHASKIHSFIVLNPV
VSFSPLLSP*PS*CIF*IVCMTSELKLIMKLQV
LIAREVHKYLILPELIWGEITSRMPWSEYLIEP
IVVHKTDCAYIYMYKK**NNGR*
Frame 1
GASRQDGGGWGFPSEAGGIGSAAAEPPPARPELSAPARLYHKKVSQICEVL*RRHKRNWGSQMVLI
DMSVDLSTQVVDHYENPRNVGSLDKTSKNVGTGL
VGAPACGDVMKLQIQVDEKGKIVDARFKTFGCG
SAIASSSLATEWVKGKTVEEALTIKNTDIAKEL
CLPPVKLHCSMLAEDAIKAALADYKLKQEPKKGE
AEKK*ALPRRSLQQATPAVSHLLCSHLRCSEAA
SSPLKSYEIRTILAVHVMTLMQAKYTVSLF*IL
WFLSAHFYRLNLVNVYFELCV*PQN*N***SCKF
**PVKCISI*FYLN*FGGKLPVECLGLNI**NQ
LLYIKQIAHIYICIKNNKIMED
Frame 2
AQAGKMAAAGAFRLRRAASALLLRSPRLPARSCRPRPDSITRRYLKSVKYCRGDTKGIGGHKWFSL
T*V*TFLLRLLIIMKILETWGPLTRHLKMLELD
WWGLQHVVT**NYRFKWMKRGRLWMLGLKHLAVV
PQLPPAH*PLNG*KERRWRKP*LSKTQISPRSS
AFLP*NCTAPCWLKMQSRPPWLITN*NKNPKKE
RQRRNEPSLGEASSRPHQLFPTCCAVTLDVQKPL
PLH*RAMRYAQYLLFTL*LSCKQNTQFHCSESC
GFFQPTFIALT*LMYILNCVYDLRTEIDNEVAS
FDSP*SA*VSNFT*IDLGGNYQ*NALV*IFDRTN
CCT*NRLRIYIYV*KIIK*WKM
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