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Sequence NM_175743
AACGGCCTGACGTCGGCGGAGGGAAGCAGGCGCAGGCTCCGTGAGGAGGCAAGAATCCGGGCTTTGCCCCTGCAATCAACCCACGGAAGCTCCGGGAATGGCGGCCAAGCACGCGGATCCTGACGTTCACATATCTCAGGGAGTTGATGACCTTGTTTTCAGAAGGTGACTCAGGTCAACACAGGGGCCCCCATCTGGTC
GACAGATGCAGTGGTTCTAGGATCTGCCAAGCATCCAGGTGGAGAGCCTGAGGAATCAGGAGCTCCAGGAACCAGGCAGTGAGGCCTTGGTCTGAGTCAG
TGTCCTCAGGTCACAGAGCAGAGGGGACGCAGACAGTGCCAACACTGAAGGTTCTGAGGGGGACAGGCTGACAAGTAGGACCCGAGGCACTGGAGGAGCA
TTGAAGGAGAAGATCTGCCTGTGGGTCTTCATTGCCCAGCTCCTGCCCGCACTCCTGCCTGCTGCCCTGACCAGAGTCATCATGCCTCTTGAGCAGAGGA
GTCAGCACTGCAAGCCTGAAGAAGGCCTTGAGGCCCGAGGAGAGGCCCTGGGCCTGGTGGGTGCGCAGGCTCCTGCTACTGAGGAGCAGCAGACCGCTTC
TTCCTCTTCTACTCTAGTGGAAGTTACCCTGGGGGAGGTGCCTGCTGCCGACTCACCGAGTCCTCCCCACAGTCCTCAGGGAGCCTCCAGCTTCTCGACT
ACCATCAACTACACTCTTTGGAGACAATCCGATGAGGGCTCCAGCAACCAAGAAGAGGAGGGGCCAAGAATGTTTCCCGACCTGGAGTCCGAGTTCCAAG
CAGCAATCAGTAGGAAGATGGTTGAGTTGGTTCATTTTCTGCTCCTCAAGTATCGAGCCAGGGAGCCGGTCACAAAGGCAGAAATGCTGGAGAGTGTCCT
CAGAAATTGCCAGGACTTCTTTCCCGTGATCTTCAGCAAAGCCTCCGAGTACTTGCAGCTGGTCTTTGGCATCGAGGTGGTGGAAGTGGTCCCCATCAGC
CACTTGTACATCCTTGTCACCTGCCTGGGCCTCTCCTACGATGGCCTGCTGGGCGACAATCAGGTCATGCCCAAGACAGGCCTCCTGATAATCGTCCTGG
CCATAATCGCAATAGAGGGCGACTGTGCCCCTGAGGAGAAAATCTGGGAGGAGCTGAGTATGTTGGAGGTGTTTGAGGGGAGGGAGGACAGTGTCTTCGC
ACATCCCAGGAAGCTGCTCATGCAAGATCTGGTGCAGGAAAACTACCTGGAGTACCGGCAGGTGCCCGGCAGTGATCCTGCATGCTACGAGTTCCTGTGG
GGTCCAAGGGCCCTCATTGAAACCAGCTATGTGAAAGTCCTGCACCATACACTAAAGATCGGTGGAGAACCTCACATTTCCTACCCACCCCTGCATGAAC
GGGCTTTGAGAGAGGGAGAAGAGTGAGTCTCAGCACATGTTGCAGCCAGGGCCAGTGGGAGGGGGTCTGGGCCAGTGCACCTTCCAGGGCCCCATCCATT
AGCTTCCACTGCCTCGTGTGATATGAGGCCCATTCCTGCCTCTTTGAAGAGAGCAGTCAGCATTCTTAGCAGTGAGTTTCTGTTCTGTTGGATGACTTTG
AGATTTATCTTTGTTTCCTGTTGGAATTGTTCAAATGTTCCTTTTAACAAATGGTTGGATGAACTTCAGCATCCAAGTTTATGAATGACAGTAGTCACAC
ATAGTGCTGTTTATATAGTTTAGGGGTAAGAGTCCTGTTTTTTATTCAGATTGGGAAATCCATTCCATTTTGTGAGTTGTCACATAATAACAGCAGTGGA
ATATGTATTTGCCTATATTGTGAACGAATTAGCAGTAAAATACATGATACAAGGAACTCAAAAGATAGTTAATTCTTGCCTTATACCTCAGTCTATTATG
TAAAATTAAAAATATGTGTATGTTTTTGCTTCTTTGAGAATGCAAAAGAAATTAAATCTGAATAAATAATTCTTCCTGTTC
- refseq_MAGEA2.F1 (forward primer)
- refseq_MAGEA2.R1 (reverse primer)
- Single exon skipping
Frame 0
NGLTSAEGSRRRLREEARIRALPLQSTHGSSGNGGQARGS*RSHISGS**PCFQKVTQVNTGAPIWS
TDAVVLGSAKHPGGEPEESGAPGTRQ*GLGLSQ
CPQVTEQRGRRQCQH*RF*GGQADK*DPRHWRS
IEGEDLPVGLHCPAPARTPACCPDQSHHAS*AEE
SALQA*RRP*GPRRGPGPGGCAGSCY*GAADRF
FLFYSSGSYPGGGACCRLTESSPQSSGSLQLLD
YHQLHSLETIR*GLQQPRRGGAKNVSRPGVRVPS
SNQ*EDG*VGSFSAPQVSSQGAGHKGRNAGECP
QKLPGLLSRDLQQSLRVLAAGLWHRGGGSGPHQ
PLVHPCHLPGPLLRWPAGRQSGHAQDRPPDNRPG
HNRNRGRLCP*GENLGGAEYVGGV*GEGGQCLR
TSQEAAHARSGAGKLPGVPAGARQ*SCMLRVPV
GSKGPH*NQLCESPAPYTKDRWRTSHFLPTPA*T
GFERGRRVSLSTCCSQGQWEGVWASAPSRAPSI
SFHCLV*YEAHSCLFEESSQHS*Q*VSVLLDDF
EIYLCFLLELFKCSF*QMVG*TSASKFMNDSSHT
*CCLYSLGVRVLFFIQIGKSIPFCELSHNNSSG
ICICLYCERISSKIHDTRNSKDS*FLPYTSVYY
VKLKICVCFCFFENAKEIKSE*IILPV
Frame 1
TA*RRRREAGAGSVRRQESGLCPCNQPTEAPGMAAKHADPDVHISQGVDDLVFRR*LRSTQGPPSG
RQMQWF*DLPSIQVESLRNQELQEPGSEALV*VS
VLRSQSRGDADSANTEGSEGDRLTSRTRGTGGA
LKEKICLWVFIAQLLPALLPAALTRVIMPLEQR
SQHCKPEEGLEARGEALGLVGAQAPATEEQQTAS
SSSTLVEVTLGEVPAADSPSPPHSPQGASSFST
TINYTLWRQSDEGSSNQEEEGPRMFPDLESEFQ
AAISRKMVELVHFLLLKYRAREPVTKAEMLESVL
RNCQDFFPVIFSKASEYLQLVFGIEVVEVVPIS
HLYILVTCLGLSYDGLLGDNQVMPKTGLLIIVL
AIIAIEGDCAPEEKIWEELSMLEVFEGREDSVFA
HPRKLLMQDLVQENYLEYRQVPGSDPACYEFLW
GPRALIETSYVKVLHHTLKIGGEPHISYPPLHE
RALREGEE*VSAHVAARASGRGSGPVHLPGPHPL
ASTASCDMRPIPASLKRAVSILSSEFLFCWMTL
RFIFVSCWNCSNVPFNKWLDELQHPSL*MTVVT
HSAVYIV*G*ESCFLFRLGNPFHFVSCHIITAVE
YVFAYIVNELAVKYMIQGTQKIVNSCLIPQSIM
*N*KYVYVFASLRMQKKLNLNK*FFLF
Frame 2
RPDVGGGKQAQAP*GGKNPGFAPAINPRKLREWRPSTRILTFTYLRELMTLFSEGDSGQHRGPHLV
DRCSGSRICQASRWRA*GIRSSRNQAVRPWSES
VSSGHRAEGTQTVPTLKVLRGTG*QVGPEALEEH
*RRRSACGSSLPSSCPHSCLLP*PESSCLLSRG
VSTASLKKALRPEERPWAWWVRRLLLLRSSRPL
LPLLL*WKLPWGRCLLPTHRVLPTVLREPPASRL
PSTTLFGDNPMRAPATKKRRGQECFPTWSPSSK
QQSVGRWLSWFIFCSSSIEPGSRSQRQKCWRVS
SEIARTSFP*SSAKPPSTCSWSLASRWWKWSPSA
TCTSLSPAWASPTMACWATIRSCPRQAS**SSW
P*SQ*RATVPLRRKSGRS*VCWRCLRGGRTVSS
HIPGSCSCKIWCRKTTWSTGRCPAVILHATSSCG
VQGPSLKPAM*KSCTIH*RSVENLTFPTHPCMN
GL*EREKSESQHMLQPGPVGGGLGQCTFQGPIH
*LPLPRVI*GPFLPL*REQSAFLAVSFCSVG*L*
DLSLFPVGIVQMFLLTNGWMNFSIQVYE*Q*SH
IVLFI*FRGKSPVFYSDWEIHSIL*VVT**QQW
NMYLPIL*TN*Q*NT*YKELKR*LILALYLSLLC
KIKNMCMFLLL*ECKRN*I*INNSSC
Sequence NM_175742
AACGGCCTGACGTCGGCGGAGGGAAGCAGGCGCAGGCTCCGTGAGGAGGCAAGGGAACTCTGGCGTAAGAGCTTTGTGTGACCAGGGCAGGGCTGGTTAGAAGTGCTCAGGGCCCAGACTCAGCCAGGAATCAAGAATCCGGGCTTTGCCCCTGCAATCAACCCACGGAAGCTCCGGGAATGGCGGCCAAGCACGCGGAT
CCTGACGTTCACATATCTCAGGGAGTTGATGACCTTGTTTTCAGAAGGTGACTCAGGTCAACACAGGGGCCCCCATCTGGTCGACAGATGCAGTGGTTCT
AGGATCTGCCAAGCATCCAGGTGGAGAGCCTGAGGTTCTGAGGGGGACAGGCTGACAAGTAGGACCCGAGGCACTGGAGGAGCATTGAAGGAGAAGATCT
GCCTGTGGGTCTTCATTGCCCAGCTCCTGCCCGCACTCCTGCCTGCTGCCCTGACCAGAGTCATCATGCCTCTTGAGCAGAGGAGTCAGCACTGCAAGCC
TGAAGAAGGCCTTGAGGCCCGAGGAGAGGCCCTGGGCCTGGTGGGTGCGCAGGCTCCTGCTACTGAGGAGCAGCAGACCGCTTCTTCCTCTTCTACTCTA
GTGGAAGTTACCCTGGGGGAGGTGCCTGCTGCCGACTCACCGAGTCCTCCCCACAGTCCTCAGGGAGCCTCCAGCTTCTCGACTACCATCAACTACACTC
TTTGGAGACAATCCGATGAGGGCTCCAGCAACCAAGAAGAGGAGGGGCCAAGAATGTTTCCCGACCTGGAGTCCGAGTTCCAAGCAGCAATCAGTAGGAA
GATGGTTGAGTTGGTTCATTTTCTGCTCCTCAAGTATCGAGCCAGGGAGCCGGTCACAAAGGCAGAAATGCTGGAGAGTGTCCTCAGAAATTGCCAGGAC
TTCTTTCCCGTGATCTTCAGCAAAGCCTCCGAGTACTTGCAGCTGGTCTTTGGCATCGAGGTGGTGGAAGTGGTCCCCATCAGCCACTTGTACATCCTTG
TCACCTGCCTGGGCCTCTCCTACGATGGCCTGCTGGGCGACAATCAGGTCATGCCCAAGACAGGCCTCCTGATAATCGTCCTGGCCATAATCGCAATAGA
GGGCGACTGTGCCCCTGAGGAGAAAATCTGGGAGGAGCTGAGTATGTTGGAGGTGTTTGAGGGGAGGGAGGACAGTGTCTTCGCACATCCCAGGAAGCTG
CTCATGCAAGATCTGGTGCAGGAAAACTACCTGGAGTACCGGCAGGTGCCCGGCAGTGATCCTGCATGCTACGAGTTCCTGTGGGGTCCAAGGGCCCTCA
TTGAAACCAGCTATGTGAAAGTCCTGCACCATACACTAAAGATCGGTGGAGAACCTCACATTTCCTACCCACCCCTGCATGAACGGGCTTTGAGAGAGGG
AGAAGAGTGAGTCTCAGCACATGTTGCAGCCAGGGCCAGTGGGAGGGGGTCTGGGCCAGTGCACCTTCCAGGGCCCCATCCATTAGCTTCCACTGCCTCG
TGTGATATGAGGCCCATTCCTGCCTCTTTGAAGAGAGCAGTCAGCATTCTTAGCAGTGAGTTTCTGTTCTGTTGGATGACTTTGAGATTTATCTTTGTTT
CCTGTTGGAATTGTTCAAATGTTCCTTTTAACAAATGGTTGGATGAACTTCAGCATCCAAGTTTATGAATGACAGTAGTCACACATAGTGCTGTTTATAT
AGTTTAGGGGTAAGAGTCCTGTTTTTTATTCAGATTGGGAAATCCATTCCATTTTGTGAGTTGTCACATAATAACAGCAGTGGAATATGTATTTGCCTAT
ATTGTGAACGAATTAGCAGTAAAATACATGATACAAGGAACTCAAAAGATAGTTAATTCTTGCCTTATACCTCAGTCTATTATGTAAAATTAAAAATATG
TGTATGTTTTTGCTTCTTTGAGAATGCAAAAGAAATTAAATCTGAATAAATAATTCTTCCTGTTC
- refseq_MAGEA2.F1 (forward primer)
- refseq_MAGEA2.R1 (reverse primer)
- Single exon skipping
Frame 0
NGLTSAEGSRRRLREEARELWRKSFV*PGQGWLEVLRAQTQPGIKNPGFAPAINPRKLREWRPSTRI
LTFTYLRELMTLFSEGDSGQHRGPHLVDRCSGS
RICQASRWRA*GSEGDRLTSRTRGTGGALKEKI
CLWVFIAQLLPALLPAALTRVIMPLEQRSQHCKP
EEGLEARGEALGLVGAQAPATEEQQTASSSSTL
VEVTLGEVPAADSPSPPHSPQGASSFSTTINYT
LWRQSDEGSSNQEEEGPRMFPDLESEFQAAISRK
MVELVHFLLLKYRAREPVTKAEMLESVLRNCQD
FFPVIFSKASEYLQLVFGIEVVEVVPISHLYIL
VTCLGLSYDGLLGDNQVMPKTGLLIIVLAIIAIE
GDCAPEEKIWEELSMLEVFEGREDSVFAHPRKL
LMQDLVQENYLEYRQVPGSDPACYEFLWGPRAL
IETSYVKVLHHTLKIGGEPHISYPPLHERALREG
EE*VSAHVAARASGRGSGPVHLPGPHPLASTAS
CDMRPIPASLKRAVSILSSEFLFCWMTLRFIFV
SCWNCSNVPFNKWLDELQHPSL*MTVVTHSAVYI
V*G*ESCFLFRLGNPFHFVSCHIITAVEYVFAY
IVNELAVKYMIQGTQKIVNSCLIPQSIM*N*KY
VYVFASLRMQKKLNLNK*FFLF
Frame 1
TA*RRRREAGAGSVRRQGNSGVRALCDQGRAG*KCSGPRLSQESRIRALPLQSTHGSSGNGGQARG
S*RSHISGS**PCFQKVTQVNTGAPIWSTDAVVL
GSAKHPGGEPEVLRGTG*QVGPEALEEH*RRRS
ACGSSLPSSCPHSCLLP*PESSCLLSRGVSTAS
LKKALRPEERPWAWWVRRLLLLRSSRPLLPLLL*
WKLPWGRCLLPTHRVLPTVLREPPASRLPSTTL
FGDNPMRAPATKKRRGQECFPTWSPSSKQQSVG
RWLSWFIFCSSSIEPGSRSQRQKCWRVSSEIART
SFP*SSAKPPSTCSWSLASRWWKWSPSATCTSL
SPAWASPTMACWATIRSCPRQAS**SSWP*SQ*
RATVPLRRKSGRS*VCWRCLRGGRTVSSHIPGSC
SCKIWCRKTTWSTGRCPAVILHATSSCGVQGPS
LKPAM*KSCTIH*RSVENLTFPTHPCMNGL*ER
EKSESQHMLQPGPVGGGLGQCTFQGPIH*LPLPR
VI*GPFLPL*REQSAFLAVSFCSVG*L*DLSLF
PVGIVQMFLLTNGWMNFSIQVYE*Q*SHIVLFI
*FRGKSPVFYSDWEIHSIL*VVT**QQWNMYLPI
L*TN*Q*NT*YKELKR*LILALYLSLLCKIKNM
CMFLLL*ECKRN*I*INNSSC
Frame 2
RPDVGGGKQAQAP*GGKGTLA*ELCVTRAGLVRSAQGPDSARNQESGLCPCNQPTEAPGMAAKHAD
PDVHISQGVDDLVFRR*LRSTQGPPSGRQMQWF
*DLPSIQVESLRF*GGQADK*DPRHWRSIEGEDL
PVGLHCPAPARTPACCPDQSHHAS*AEESALQA
*RRP*GPRRGPGPGGCAGSCY*GAADRFFLFYS
SGSYPGGGACCRLTESSPQSSGSLQLLDYHQLHS
LETIR*GLQQPRRGGAKNVSRPGVRVPSSNQ*E
DG*VGSFSAPQVSSQGAGHKGRNAGECPQKLPG
LLSRDLQQSLRVLAAGLWHRGGGSGPHQPLVHPC
HLPGPLLRWPAGRQSGHAQDRPPDNRPGHNRNR
GRLCP*GENLGGAEYVGGV*GEGGQCLRTSQEA
AHARSGAGKLPGVPAGARQ*SCMLRVPVGSKGPH
*NQLCESPAPYTKDRWRTSHFLPTPA*TGFERG
RRVSLSTCCSQGQWEGVWASAPSRAPSISFHCL
V*YEAHSCLFEESSQHS*Q*VSVLLDDFEIYLCF
LLELFKCSF*QMVG*TSASKFMNDSSHT*CCLY
SLGVRVLFFIQIGKSIPFCELSHNNSSGICICL
YCERISSKIHDTRNSKDS*FLPYTSVYYVKLKIC
VCFCFFENAKEIKSE*IILPV
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