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Sequence NM_021048
GAGAAGCGAGGTTCTCGTTCTGAGGGACAGGCTTGAGATCGGCTGAAGAGAGCGGGCCCAGGCTCTGTGAGGAGGCAAGGGAGGTGAGAACCTTGCTCTCAGAGGGTGACTCAAGTCAACACAGGGAACCCCTCTTTTCTACAGACACAGTGGGTCGCAGGATCTGACAAGAGTCCAGGTTCTCAGGGGACAGGGAGAGC
AAGAGGTCAAGAGCTGTGGGACACCACAGAGCAGCACTGAAGGAGAAGACCTGCCTGTGGGTCCCCATCGCCCAAGTCCTGCCCACACTCCCACCTGCTA
CCCTGATCAGAGTCATCATGCCTCGAGCTCCAAAGCGTCAGCGCTGCATGCCTGAAGAAGATCTTCAATCCCAAAGTGAGACACAGGGCCTCGAGGGTGC
ACAGGCTCCCCTGGCTGTGGAGGAGGATGCTTCATCATCCACTTCCACCAGCTCCTCTTTTCCATCCTCTTTTCCCTCCTCCTCCTCTTCCTCCTCCTCC
TCCTGCTATCCTCTAATACCAAGCACCCCAGAGGAGGTTTCTGCTGATGATGAGACACCAAATCCTCCCCAGAGTGCTCAGATAGCCTGCTCCTCCCCCT
CGGTCGTTGCTTCCCTTCCATTAGATCAATCTGATGAGGGCTCCAGCAGCCAAAAGGAGGAGAGTCCAAGCACCCTACAGGTCCTGCCAGACAGTGAGTC
TTTACCCAGAAGTGAGATAGATGAAAAGGTGACTGATTTGGTGCAGTTTCTGCTCTTCAAGTATCAAATGAAGGAGCCGATCACAAAGGCAGAAATACTG
GAGAGTGTCATAAGAAATTATGAAGACCACTTCCCTTTGTTGTTTAGTGAAGCCTCCGAGTGCATGCTGCTGGTCTTTGGCATTGATGTAAAGGAAGTGG
ATCCCACTGGCCACTCCTTTGTCCTTGTCACCTCCCTGGGCCTCACCTATGATGGGATGCTGAGTGATGTCCAGAGCATGCCCAAGACTGGCATTCTCAT
ACTTATCCTAAGCATAGTCTTCATAGAGGGCTACTGCACCCCTGAGGAGGTCATCTGGGAAGCACTGAATATGATGGGGCTGTATGATGGGATGGAGCAC
CTCATTTATGGGGAGCCCAGGAAGCTGCTCACCCAAGATTGGGTGCAGGAAAACTACCTGGAGTACCGGCAGGTGCCTGGCAGTGATCCTGCACGGTATG
AGTTTCTGTGGGGTCCAAGGGCTCATGCTGAAATTAGGAAGATGAGTCTCCTGAAATTTTTGGCCAAGGTAAATGGGAGTGATCCAAGATCCTTCCCACT
GTGGTATGAGGAGGCTTTGAAAGATGAGGAAGAGAGAGCCCAGGACAGAATTGCCACCACAGATGATACTACTGCCATGGCCAGTGCAAGTTCTAGCGCT
ACAGGTAGCTTCTCCTACCCTGAATAAAGTAAGACAGATTCTTCACTGTGTTTTAAAAGGCAAGTCAAATACCACATGATTTTACTCATATGTGGAATCT
AAAAAAAAAAAAAAAAAAA
- refseq_MAGEA10.F1 (forward primer)
- refseq_MAGEA10.R1 (reverse primer)
- Single exon skipping
Frame 0
EKRGSRSEGQA*DRLKRAGPGSVRRQGR*EPCSQRVTQVNTGNPSFLQTQWVAGSDKSPGSQGTGRA
RGQELWDTTEQH*RRRPACGSPSPKSCPHSHLL
P*SESSCLELQSVSAACLKKIFNPKVRHRASRV
HRLPWLWRRMLHHPLPPAPLFHPLFPPPPLPPPP
PAIL*YQAPQRRFLLMMRHQILPRVLR*PAPPP
RSLLPFH*INLMRAPAAKRRRVQAPYRSCQTVS
LYPEVR*MKR*LIWCSFCSSSIK*RSRSQRQKYW
RVS*EIMKTTSLCCLVKPPSACCWSLALM*RKW
IPLATPLSLSPPWASPMMGC*VMSRACPRLAFS
YLS*A*SS*RATAPLRRSSGKH*I*WGCMMGWST
SFMGSPGSCSPKIGCRKTTWSTGRCLAVILHGM
SFCGVQGLMLKLGR*VS*NFWPR*MGVIQDPSH
CGMRRL*KMRKREPRTELPPQMILLPWPVQVLAL
QVASPTLNKVRQILHCVLKGKSNTT*FYSYVES
KKKKKK
Frame 1
RSEVLVLRDRLEIG*RERAQAL*GGKGGENLALRG*LKSTQGTPLFYRHSGSQDLTRVQVLRGQGE
QEVKSCGTPQSSTEGEDLPVGPHRPSPAHTPTCY
PDQSHHASSSKASALHA*RRSSIPK*DTGPRGC
TGSPGCGGGCFIIHFHQLLFSILFSLLLLFLLL
LLLSSNTKHPRGGFC***DTKSSPECSDSLLLPL
GRCFPSIRSI**GLQQPKGGESKHPTGPARQ*V
FTQK*DR*KGD*FGAVSALQVSNEGADHKGRNT
GECHKKL*RPLPFVV**SLRVHAAGLWH*CKGSG
SHWPLLCPCHLPGPHL*WDAE*CPEHAQDWHSH
TYPKHSLHRGLLHP*GGHLGSTEYDGAV*WDGA
PHLWGAQEAAHPRLGAGKLPGVPAGAWQ*SCTV*
VSVGSKGSC*N*EDESPEIFGQGKWE*SKILPT
VV*GGFER*GRESPGQNCHHR*YYCHGQCKF*R
YR*LLLP*IK*DRFFTVF*KASQIPHDFTHMWNL
KKKKKK
Frame 2
EARFSF*GTGLRSAEESGPRLCEEAREVRTLLSEGDSSQHREPLFSTDTVGRRI*QESRFSGDRES
KRSRAVGHHRAALKEKTCLWVPIAQVLPTLPPA
TLIRVIMPRAPKRQRCMPEEDLQSQSETQGLEGA
QAPLAVEEDASSSTSTSSSFPSSFPSSSSSSSS
SCYPLIPSTPEEVSADDETPNPPQSAQIACSSP
SVVASLPLDQSDEGSSSQKEESPSTLQVLPDSES
LPRSEIDEKVTDLVQFLLFKYQMKEPITKAEIL
ESVIRNYEDHFPLLFSEASECMLLVFGIDVKEV
DPTGHSFVLVTSLGLTYDGMLSDVQSMPKTGILI
LILSIVFIEGYCTPEEVIWEALNMMGLYDGMEH
LIYGEPRKLLTQDWVQENYLEYRQVPGSDPARY
EFLWGPRAHAEIRKMSLLKFLAKVNGSDPRSFPL
WYEEALKDEEERAQDRIATTDDTTAMASASSSA
TGSFSYPE*SKTDSSLCFKRQVKYHMILLICGI
*KKKKK
Sequence NM_001011543
GAGAAGCGAGGTTCTCGTTCTGAGGGACAGGCTTGAGATCGGCTGAAGAGAGCGGGCCCAGGCTCTGTGAGGAGGCAAGGGAGGTGAGAACCTTGCTCTCAGAGGGTGACTCAAGTCAACACAGGGAACCCCTCTTTTCTACAGACACAGTGGGTCGCAGGATCTGACAAGAGTCCAGGAGCTCCAGGGACTGTGAGGTG
AGGCCTTGGTCTAAGGCAGTGTTTTCAGGTCACAGAGCAGAAAGGGCCCAGACAGTGCCAGGAGTCAAGGTTCTCAGGGGACAGGGAGAGCAAGAGGTCA
AGAGCTGTGGGACACCACAGAGCAGCACTGAAGGAGAAGACCTGCCTGTGGGTCCCCATCGCCCAAGTCCTGCCCACACTCCCACCTGCTACCCTGATCA
GAGTCATCATGCCTCGAGCTCCAAAGCGTCAGCGCTGCATGCCTGAAGAAGATCTTCAATCCCAAAGTGAGACACAGGGCCTCGAGGGTGCACAGGCTCC
CCTGGCTGTGGAGGAGGATGCTTCATCATCCACTTCCACCAGCTCCTCTTTTCCATCCTCTTTTCCCTCCTCCTCCTCTTCCTCCTCCTCCTCCTGCTAT
CCTCTAATACCAAGCACCCCAGAGGAGGTTTCTGCTGATGATGAGACACCAAATCCTCCCCAGAGTGCTCAGATAGCCTGCTCCTCCCCCTCGGTCGTTG
CTTCCCTTCCATTAGATCAATCTGATGAGGGCTCCAGCAGCCAAAAGGAGGAGAGTCCAAGCACCCTACAGGTCCTGCCAGACAGTGAGTCTTTACCCAG
AAGTGAGATAGATGAAAAGGTGACTGATTTGGTGCAGTTTCTGCTCTTCAAGTATCAAATGAAGGAGCCGATCACAAAGGCAGAAATACTGGAGAGTGTC
ATAAGAAATTATGAAGACCACTTCCCTTTGTTGTTTAGTGAAGCCTCCGAGTGCATGCTGCTGGTCTTTGGCATTGATGTAAAGGAAGTGGATCCCACTG
GCCACTCCTTTGTCCTTGTCACCTCCCTGGGCCTCACCTATGATGGGATGCTGAGTGATGTCCAGAGCATGCCCAAGACTGGCATTCTCATACTTATCCT
AAGCATAGTCTTCATAGAGGGCTACTGCACCCCTGAGGAGGTCATCTGGGAAGCACTGAATATGATGGGGCTGTATGATGGGATGGAGCACCTCATTTAT
GGGGAGCCCAGGAAGCTGCTCACCCAAGATTGGGTGCAGGAAAACTACCTGGAGTACCGGCAGGTGCCTGGCAGTGATCCTGCACGGTATGAGTTTCTGT
GGGGTCCAAGGGCTCATGCTGAAATTAGGAAGATGAGTCTCCTGAAATTTTTGGCCAAGGTAAATGGGAGTGATCCAAGATCCTTCCCACTGTGGTATGA
GGAGGCTTTGAAAGATGAGGAAGAGAGAGCCCAGGACAGAATTGCCACCACAGATGATACTACTGCCATGGCCAGTGCAAGTTCTAGCGCTACAGGTAGC
TTCTCCTACCCTGAATAAAGTAAGACAGATTCTTCACTGTGTTTTAAAAGGCAAGTCAAATACCACATGATTTTACTCATATGTGGAATCTAAAAAAAAA
AAAAAAAAAA
- refseq_MAGEA10.F1 (forward primer)
- refseq_MAGEA10.R1 (reverse primer)
- Single exon skipping
Frame 0
EKRGSRSEGQA*DRLKRAGPGSVRRQGR*EPCSQRVTQVNTGNPSFLQTQWVAGSDKSPGAPGTVR*
GLGLRQCFQVTEQKGPRQCQESRFSGDRESKRS
RAVGHHRAALKEKTCLWVPIAQVLPTLPPATLI
RVIMPRAPKRQRCMPEEDLQSQSETQGLEGAQAP
LAVEEDASSSTSTSSSFPSSFPSSSSSSSSSCY
PLIPSTPEEVSADDETPNPPQSAQIACSSPSVV
ASLPLDQSDEGSSSQKEESPSTLQVLPDSESLPR
SEIDEKVTDLVQFLLFKYQMKEPITKAEILESV
IRNYEDHFPLLFSEASECMLLVFGIDVKEVDPT
GHSFVLVTSLGLTYDGMLSDVQSMPKTGILILIL
SIVFIEGYCTPEEVIWEALNMMGLYDGMEHLIY
GEPRKLLTQDWVQENYLEYRQVPGSDPARYEFL
WGPRAHAEIRKMSLLKFLAKVNGSDPRSFPLWYE
EALKDEEERAQDRIATTDDTTAMASASSSATGS
FSYPE*SKTDSSLCFKRQVKYHMILLICGI*KK
KKK
Frame 1
RSEVLVLRDRLEIG*RERAQAL*GGKGGENLALRG*LKSTQGTPLFYRHSGSQDLTRVQELQGL*G
EALV*GSVFRSQSRKGPDSARSQGSQGTGRARGQ
ELWDTTEQH*RRRPACGSPSPKSCPHSHLLP*S
ESSCLELQSVSAACLKKIFNPKVRHRASRVHRL
PWLWRRMLHHPLPPAPLFHPLFPPPPLPPPPPAI
L*YQAPQRRFLLMMRHQILPRVLR*PAPPPRSL
LPFH*INLMRAPAAKRRRVQAPYRSCQTVSLYP
EVR*MKR*LIWCSFCSSSIK*RSRSQRQKYWRVS
*EIMKTTSLCCLVKPPSACCWSLALM*RKWIPL
ATPLSLSPPWASPMMGC*VMSRACPRLAFSYLS
*A*SS*RATAPLRRSSGKH*I*WGCMMGWSTSFM
GSPGSCSPKIGCRKTTWSTGRCLAVILHGMSFC
GVQGLMLKLGR*VS*NFWPR*MGVIQDPSHCGM
RRL*KMRKREPRTELPPQMILLPWPVQVLALQVA
SPTLNKVRQILHCVLKGKSNTT*FYSYVESKKK
KKK
Frame 2
EARFSF*GTGLRSAEESGPRLCEEAREVRTLLSEGDSSQHREPLFSTDTVGRRI*QESRSSRDCEV
RPWSKAVFSGHRAERAQTVPGVKVLRGQGEQEV
KSCGTPQSSTEGEDLPVGPHRPSPAHTPTCYPDQ
SHHASSSKASALHA*RRSSIPK*DTGPRGCTGS
PGCGGGCFIIHFHQLLFSILFSLLLLFLLLLLL
SSNTKHPRGGFC***DTKSSPECSDSLLLPLGRC
FPSIRSI**GLQQPKGGESKHPTGPARQ*VFTQ
K*DR*KGD*FGAVSALQVSNEGADHKGRNTGEC
HKKL*RPLPFVV**SLRVHAAGLWH*CKGSGSHW
PLLCPCHLPGPHL*WDAE*CPEHAQDWHSHTYP
KHSLHRGLLHP*GGHLGSTEYDGAV*WDGAPHL
WGAQEAAHPRLGAGKLPGVPAGAWQ*SCTV*VSV
GSKGSC*N*EDESPEIFGQGKWE*SKILPTVV*
GGFER*GRESPGQNCHHR*YYCHGQCKF*RYR*
LLLP*IK*DRFFTVF*KASQIPHDFTHMWNLKKK
KKK
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