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Sequences

Sequence NM_013436

AGGTGACCGAGGGCCCGTCCGGGGCTGCGGGAAGCGGCCTCGTTCTCAGCCGCCGGAGACGCCGCCGCCGCCGCCGCCGCCGCCACACCTAGTGGAGCAG CCGGGGAAGGCGGCTCGTTGGGGGCTGGGGCGGAGAGCGCCGGGGGTGGGGGCGGAAGGGCGGCGGGCGGAAGGCAGGAGGCTGCCGGGGCGCGGGCTGC TGCGGGAGAAGGGGCTCCGAGGAGTCCGCCGCGGCTCGCTCTGTCGCCGGCGCGGGATTGGGGCGCGAGGGCCATGGGCGCGCTCTCCTAAGGCGGAGGT CGCGGGCGGGAGGGGAGGAGGCCCGAGAGAGGCTGCTGCGAAGGCCGCGGGCCCGTGACTGGGCGCGAGGCGGCCGGCGGCGGCGGCGGCACCAGCACCA CCATGTCGCGCTCAGTGCTGCAGCCCAGTCAGCAGAAGCTGGCGGAGAAGCTCACCATCCTCAACGACCGGGGCGTCGGCATGCTCACCCGCCTCTACAA CATCAAGAAGGCATGTGGAGACCCCAAGGCAAAACCATCCTATCTTATCGACAAAAACCTGGAATCTGCTGTGAAATTCATAGTCAGAAAATTCCCTGCT GTAGAAACCCGCAACAACAATCAACAGCTTGCACAACTACAGAAAGAAAAATCAGAGATTCTGAAAAATCTGGCATTATATTACTTCACATTTGTAGATG TTATGGAATTTAAGGACCATGTTTGTGAATTGCTGAATACTATTGACGTTTGCCAAGTCTTCTTTGATATTACTGTAAACTTTGATTTAACAAAGAACTA CTTAGATTTAATTATAACCTATACAACACTAATGATACTGCTGTCTCGAATTGAAGAAAGGAAGGCAATCATTGGATTATACAACTATGCCCATGAAATG ACTCATGGAGCAAGTGACAGAGAATACCCACGCCTTGGCCAGATGATTGTGGATTATGAAAACCCTTTAAAGAAGATGATGGAAGAATTTGTACCCCATA GCAAGTCTCTTTCAGATGCACTAATTTCTCTTCAAATGGTATATCCTCGAAGGAATCTTTCAGCTGACCAGTGGAGAAATGCCCAGTTATTGAGCCTCAT CAGTGCACCTAGTACAATGCTTAATCCAGCACAGTCCGACACTATGCCTTGTGAATACCTCTCTTTGGATGCAATGGAAAAGTGGATTATCTTTGGCTTT ATTTTGTGCCATGGGATCCTAAATACTGACGCTACAGCACTGAACCTTTGGAAACTAGCTCTTCAAAGTAGCTCTTGCCTCTCTCTCTTTCGGGATGAAG TTTTCCACATTCACAAAGCTGCAGAAGACTTATTTGTAAACATACGAGGCTATAATAAACGTATTAATGACATAAGAGAATGCAAGGAGGCAGCCGTGTC ACATGCTGGTTCAATGCACAGAGAAAGACGCAAGTTTTTAAGATCTGCACTGAAGGAATTGGCTACTGTCCTCTCTGATCAACCTGGATTGCTAGGTCCC AAGGCACTTTTTGTTTTTATGGCATTATCCTTTGCCCGTGATGAAATCATCTGGCTACTTCGTCATGCAGATAACATGCCAAAGAAGAGTGCAGACGACT TTATAGATAAGCACATTGCTGAATTAATATTTTACATGGAAGAACTTAGAGCACATGTGAGGAAATACGGACCTGTAATGCAGAGGTATTACGTGCAGTA CCTTTCTGGCTTTGATGCTGTTGTCCTCAATGAACTCGTGCAGAATCTTTCTGTTTGCCCTGAAGATGAATCAATCATCATGTCCTCTTTTGTTAACACT ATGACTTCCCTAAGTGTAAAACAAGTTGAAGATGGGGAAGTATTTGATTTCAGAGGAATGAGATTAGATTGGTTTAGGTTACAGGCATATACTAGTGTCT CAAAGGCTTCACTTGGCCTTGCAGATCACAGAGAACTTGGAAAGATGATGAATACAATAATTTTTCATACAAAAATGGTAGATTCCTTGGTGGAAATGTT GGTGGAAACATCAGATCTCTCCATATTTTGTTTTTATAGTCGTGCTTTTGAGAAGATGTTTCAACAGTGTTTGGAGTTACCCTCTCAATCAAGATACTCA ATTGCATTTCCACTACTTTGCACTCATTTTATGAGTTGCACGCATGAACTATGTCCAGAAGAGCGACATCATATTGGAGATCGCAGTCTTTCCTTATGTA ATATGTTCCTAGATGAAATGGCCAAACAAGCTCGAAATCTCATCACTGATATTTGCACAGAACAGTGTACCCTTAGTGACCAGTTGCTACCCAAGCATTG TGCCAAAACTATCAGTCAAGCAGTGAATAAGAAATCAAAAAAGCAGACTGGTAAGAAAGGGGAACCTGAAAGGGAGAAACCAGGTGTTGAGAGCATGAGG AAAAACAGGCTGGTTGTGACCAACCTTGATAAATTGCACACTGCACTTTCTGAGTTATGCTTCTCTATAAATTATGTACCAAACATGGTGGTATGGGAAC ATACCTTTACCCCACGAGAATATTTGACTTCTCATCTGGAAATACGCTTTACCAAGTCAATTGTTGGGATGACTATGTATAATCAAGCCACACAGGAAAT TGCAAAACCTTCAGAACTTCTAACAAGTGTAAGAGCATACATGACCGTACTCCAGTCAATAGAAAACTATGTGCAGATTGATATTACAAGAGTATTTAAT AATGTGCTTCTTCAACAAACACAACATTTAGACAGTCATGGAGAGCCAACCATTACAAGTCTATACACAAATTGGTATTTGGAAACTTTGTTACGACAAG TCAGCAATGGCCATATAGCATATTTTCCTGCAATGAAAGCGTTTGTGAACTTACCTACAGAAAATGAATTAACATTCAATGCAGAGGAATATTCTGACAT ATCAGAAATGAGGTCATTATCAGAACTACTAGGCCCATATGGTATGAAGTTTCTAAGTGAAAGCCTTATGTGGCATATTTCATCACAAGTTGCTGAACTT AAGAAACTTGTGGTGGAGAATGTTGATGTGTTAACACAAATGAGGACCAGCTTTGACAAACCAGACCAGATGGCTGCACTGTTTAAAAGATTATCATCTG TTGACAGTGTCTTGAAGAGGATGACAATAATTGGTGTAATTTTATCCTTCCGATCATTGGCACAAGAAGCACTTAGAGATGTCTTATCCTACCACATTCC TTTTCTTGTAAGTTCAATTGAAGATTTTAAGGATCACATTCCAAGGGAAACTGATATGAAGGTTGCAATGAATGTGTATGAGTTATCATCAGCTGCCGGA TTACCTTGTGAGATTGATCCTGCATTGGTCGTAGCTCTTTCTTCACAAAAATCGGAAAACATTAGTCCAGAAGAAGAGTATAAAATTGCCTGCCTTCTCA TGGTGTTTGTGGCAGTTTCTTTGCCAACACTGGCCAGTAATGTGATGTCTCAGTACAGCCCTGCTATAGAAGGGCATTGCAACAACATACATTGCTTGGC CAAAGCCATCAACCAGATTGCTGCAGCTTTGTTTACAATTCACAAAGGAAGCATTGAAGACCGTCTTAAAGAATTTCTGGCGCTTGCATCCTCCAGTCTA CTGAAAATTGGCCAGGAGACAGATAAAACTACAACAAGAAATAGAGAATCTGTTTATTTACTGCTAGATATGATTGTACAAGAATCTCCATTCCTTACAA TGGATCTTTTGGAATCTTGTTTTCCTTATGTCTTGCTGAGAAATGCATACCATGCTGTCTACAAACAAAGTGTTACATCTTCTGCATAAAATTACCTACT TAATCAAGATAAGCACGCATTTTTGTTGCCTTGGTTTTACCTGTAGACTGTGGAACTATTTTACCTTAAGACCTGAAAAAGTTTTGTGGATTATAAATTT CTTTCATACGGTTGTATTTTCTGATCATTGGTTTCTTAATATGGTTGTACTACAGTATACTTGGTTGATTTAGGTTGCACATTCACTGAATTCACTGAGA TTATTCCTATAATTTTAAAGTATCATTTATTTGAAAAACATACATTATCAACATGTTTTTGATATTTGATAATGAAAAAAATCTTTGCTTGTTTATTTCT GAAAAAGAACTGTATTTAGTGATTATTTTAGATAGTGATATTATAGTATTCATCTGTGTGTAAATTATTTCATATAGGGAAGAGTTCTGATCTGTACCTA TGGTTCTTATTGAAAACAACATTGGATGTGCATTTCTGTGATGTTATGAATACATTTCTACTTTATTTTGAAACATTTGCCAAACTAAATACTGTAACAC TGTATAACATTTAAAAATGTTAAAGAACTGCTTAGTATTAGAAGCAGATCATTTCCCAAAATTCTAAGAGCAGCAGCATATGTTGTTGCTTGTATAAAGC CTAGCGATAATTTTTAGACTAACTTCCATGGTGCCCTGTTGGCATTAGCACTACCATTGTACCCTGCTGTATAATAAACAATCTTAGACATTTATCAACT GTTGATACAAATGTTAGTCCCTAACCACTTTTTATATATGTTTTAAATTTTTGAAATTCAAGTGTACCTGCCATAACATAAAATAAACACTAGACTGTAT CACACTTCGATTGA

refseq_NCKAP1.F1 (forward primer)
refseq_NCKAP1.R1 (reverse primer)
Single exon skipping

Frame 0

R*PRARPGLREAASFSAAGDAAAAAAAATPSGA AGEGGSLGAGAESAGGGGGRAAGGRQEAAGARAA AGEGAPRSPPRLALSPARDWGARAMGALS*GGG RGREGRRPERGCCEGRGPVTGREAAGGGGGTST TMSRSVLQPSQQKLAEKLTILNDRGVGMLTRLYN IKKACGDPKAKPSYLIDKNLESAVKFIVRKFPA VETRNNNQQLAQLQKEKSEILKNLALYYFTFVD VMEFKDHVCELLNTIDVCQVFFDITVNFDLTKNY LDLIITYTTLMILLSRIEERKAIIGLYNYAHEM THGASDREYPRLGQMIVDYENPLKKMMEEFVPH SKSLSDALISLQMVYPRRNLSADQWRNAQLLSLI SAPSTMLNPAQSDTMPCEYLSLDAMEKWIIFGF ILCHGILNTDATALNLWKLALQSSSCLSLFRDE VFHIHKAAEDLFVNIRGYNKRINDIRECKEAAVS HAGSMHRERRKFLRSALKELATVLSDQPGLLGP KALFVFMALSFARDEIIWLLRHADNMPKKSADD FIDKHIAELIFYMEELRAHVRKYGPVMQRYYVQY LSGFDAVVLNELVQNLSVCPEDESIIMSSFVNT MTSLSVKQVEDGEVFDFRGMRLDWFRLQAYTSV SKASLGLADHRELGKMMNTIIFHTKMVDSLVEML VETSDLSIFCFYSRAFEKMFQQCLELPSQSRYS IAFPLLCTHFMSCTHELCPEERHHIGDRSLSLC NMFLDEMAKQARNLITDICTEQCTLSDQLLPKHC AKTISQAVNKKSKKQTGKKGEPEREKPGVESMR KNRLVVTNLDKLHTALSELCFSINYVPNMVVWE HTFTPREYLTSHLEIRFTKSIVGMTMYNQATQEI AKPSELLTSVRAYMTVLQSIENYVQIDITRVFN NVLLQQTQHLDSHGEPTITSLYTNWYLETLLRQ VSNGHIAYFPAMKAFVNLPTENELTFNAEEYSDI SEMRSLSELLGPYGMKFLSESLMWHISSQVAEL KKLVVENVDVLTQMRTSFDKPDQMAALFKRLSS VDSVLKRMTIIGVILSFRSLAQEALRDVLSYHIP FLVSSIEDFKDHIPRETDMKVAMNVYELSSAAG LPCEIDPALVVALSSQKSENISPEEEYKIACLL MVFVAVSLPTLASNVMSQYSPAIEGHCNNIHCLA KAINQIAAALFTIHKGSIEDRLKEFLALASSSL LKIGQETDKTTTRNRESVYLLLDMIVQESPFLT MDLLESCFPYVLLRNAYHAVYKQSVTSSA*NYLL NQDKHAFLLPWFYL*TVELFYLKT*KSFVDYKF LSYGCIF*SLVS*YGCTTVYLVDLGCTFTEFTE IIPIILKYHLFEKHTLSTCF*YLIMKKIFACLFL KKNCI**LF*IVIL*YSSVCKLFHIGKSSDLYL WFLLKTTLDVHFCDVMNTFLLYFETFAKLNTVT LYNI*KC*RTA*Y*KQIISQNSKSSSICCCLYKA *R*FLD*LPWCPVGISTTIVPCCIINNLRHLST VDTNVSP*PLFIYVLNF*NSSVPAIT*NKH*TV SHFD*

Frame 1

GDRGPVRGCGKRPRSQPPETPPPPPPPPHLVEQ PGKAARWGLGRRAPGVGAEGRRAEGRRLPGRGL LREKGLRGVRRGSLCRRRGIGARGPWARSPKAEV AGGRGGGPREAAAKAAGP*LGARRPAAAAAPAP PCRAQCCSPVSRSWRRSSPSSTTGASACSPAST TSRRHVETPRQNHPILSTKTWNLL*NS*SENSLL *KPATTINSLHNYRKKNQRF*KIWHYITSHL*M LWNLRTMFVNC*ILLTFAKSSLILL*TLI*QRT T*I*L*PIQH**YCCLELKKGRQSLDYTTMPMK* LMEQVTENTHALAR*LWIMKTL*RR*WKNLYPI ASLFQMH*FLFKWYILEGIFQLTSGEMPSY*AS SVHLVQCLIQHSPTLCLVNTSLWMQWKSGLSLAL FCAMGS*ILTLQH*TFGN*LFKVALASLSFGMK FSTFTKLQKTYL*TYEAIINVLMT*ENARRQPC HMLVQCTEKDASF*DLH*RNWLLSSLINLDC*VP RHFLFLWHYPLPVMKSSGYFVMQITCQRRVQTT L*ISTLLN*YFTWKNLEHM*GNTDL*CRGITCS TFLALMLLSSMNSCRIFLFALKMNQSSCPLLLTL *LP*V*NKLKMGKYLISEE*D*IGLGYRHILVS QRLHLALQITENLER**IQ*FFIQKW*IPWWKC WWKHQISPYFVFIVVLLRRCFNSVWSYPLNQDTQ LHFHYFALIL*VARMNYVQKSDIILEIAVFPYV ICS*MKWPNKLEISSLIFAQNSVPLVTSCYPSI VPKLSVKQ*IRNQKSRLVRKGNLKGRNQVLRA*G KTGWL*PTLINCTLHFLSYASL*IMYQTWWYGN IPLPHENI*LLIWKYALPSQLLG*LCIIKPHRK LQNLQNF*QV*EHT*PYSSQ*KTMCRLILQEYLI MCFFNKHNI*TVMESQPLQVYTQIGIWKLCYDK SAMAI*HIFLQ*KRL*TYLQKMN*HSMQRNILT YQK*GHYQNY*AHMV*SF*VKALCGIFHHKLLNL RNLWWRMLMC*HK*GPALTNQTRWLHCLKDYHL LTVS*RG*Q*LV*FYPSDHWHKKHLEMSYPTTF LFL*VQLKILRITFQGKLI*RLQ*MCMSYHQLPD YLVRLILHWS*LFLHKNRKTLVQKKSIKLPAFS WCLWQFLCQHWPVM*CLSTALL*KGIATTYIAW PKPSTRLLQLCLQFTKEALKTVLKNFWRLHPPVY *KLARRQIKLQQEIENLFIYC*I*LYKNLHSLQ WIFWNLVFLMSC*EMHTMLSTNKVLHLLHKITY LIKISTHFCCLGFTCRLWNYFTLRPEKVLWIINF FHTVVFSDHWFLNMVVLQYTWLI*VAHSLNSLR LFL*F*SIIYLKNIHYQHVFDI***KKSLLVYF *KRTVFSDYFR**YYSIHLCVNYFI*GRVLICTY GSY*KQHWMCISVML*IHFYFILKHLPN*IL*H CITFKNVKELLSIRSRSFPKILRAAAYVVACIK PSDNF*TNFHGALLALALPLYPAV**TILDIYQL LIQMLVPNHFLYMF*IFEIQVYLP*HKINTRLY HTSI

Frame 2

VTEGPSGAAGSGLVLSRRRRRRRRRRRHT*WSS RGRRLVGGWGGERRGWGRKGGGRKAGGCRGAGC CGRRGSEESAAARSVAGAGLGREGHGRALLRRR SRAGGEEARERLLRRPRARDWARGGRRRRRHQHH HVALSAAAQSAEAGGEAHHPQRPGRRHAHPPLQ HQEGMWRPQGKTILSYRQKPGICCEIHSQKIPC CRNPQQQSTACTTTERKIRDSEKSGIILLHICRC YGI*GPCL*IAEYY*RLPSLL*YYCKL*FNKEL LRFNYNLYNTNDTAVSN*RKEGNHWIIQLCP*N DSWSK*QRIPTPWPDDCGL*KPFKEDDGRICTP* QVSFRCTNFSSNGISSKESFS*PVEKCPVIEPH QCT*YNA*SSTVRHYAL*IPLFGCNGKVDYLWL YFVPWDPKY*RYSTEPLETSSSK*LLPLSLSG*S FPHSQSCRRLICKHTRL**TY**HKRMQGGSRV TCWFNAQRKTQVFKICTEGIGYCPL*STWIARS QGTFCFYGIILCP**NHLATSSCR*HAKEECRRL YR*AHC*INILHGRT*STCEEIRTCNAEVLRAV PFWL*CCCPQ*TRAESFCLP*R*INHHVLFC*H YDFPKCKTS*RWGSI*FQRNEIRLV*VTGIY*CL KGFTWPCRSQRTWKDDEYNNFSYKNGRFLGGNV GGNIRSLHILFL*SCF*EDVSTVFGVTLSIKIL NCISTTLHSFYELHA*TMSRRATSYWRSQSFLM* YVPR*NGQTSSKSHH*YLHRTVYP**PVATQAL CQNYQSSSE*EIKKADW*ERGT*KGETRC*EHE EKQAGCDQP**IAHCTF*VMLLYKLCTKHGGMGT YLYPTRIFDFSSGNTLYQVNCWDDYV*SSHTGN CKTFRTSNKCKSIHDRTPVNRKLCAD*YYKSI* *CASSTNTTFRQSWRANHYKSIHKLVFGNFVTTS QQWPYSIFSCNESVCELTYRK*INIQCRGIF*H IRNEVIIRTTRPIWYEVSK*KPYVAYFITSC*T *ETCGGEC*CVNTNEDQL*QTRPDGCTV*KIIIC *QCLEEDDNNWCNFILPIIGTRST*RCLILPHS FSCKFN*RF*GSHSKGN*YEGCNECV*VIISCR ITL*D*SCIGRSSFFTKIGKH*SRRRV*NCLPSH GVCGSFFANTGQ*CDVSVQPCYRRALQQHTLLG QSHQPDCCSFVYNSQRKH*RPS*RISGACILQS TENWPGDR*NYNKK*RICLFTARYDCTRISIPYN GSFGILFSLCLAEKCIPCCLQTKCYIFCIKLPT *SR*ARIFVALVLPVDCGTILP*DLKKFCGL*I SFIRLYFLIIGFLIWLYYSILG*FRLHIH*IH*D YSYNFKVSFI*KTYIINMFLIFDNEKNLCLFIS EKELYLVIILDSDIIVFICV*IISYREEF*SVP MVLIENNIGCAFL*CYEYISTLF*NICQTKYCNT V*HLKMLKNCLVLEADHFPKF*EQQHMLLLV*S LAIIFRLTSMVPCWH*HYHCTLLYNKQS*TFIN C*YKC*SLTTFYICFKFLKFKCTCHNIK*TLDCI TLRL

Sequence NM_205842

AGGTGACCGAGGGCCCGTCCGGGGCTGCGGGAAGCGGCCTCGTTCTCAGCCGCCGGAGACGCCGCCGCCGCCGCCGCCGCCGCCACACCTAGTGGAGCAG CCGGGGAAGGCGGCTCGTTGGGGGCTGGGGCGGAGAGCGCCGGGGGTGGGGGCGGAAGGGCGGCGGGCGGAAGGCAGGAGGCTGCCGGGGCGCGGGCTGC TGCGGGAGAAGGGGCTCCGAGGAGTCCGCCGCGGCTCGCTCTGTCGCCGGCGCGGGATTGGGGCGCGAGGGCCATGGGCGCGCTCTCCTAAGGCGGAGGT CGCGGGCGGGAGGGGAGGAGGCCCGAGAGAGGCTGCTGCGAAGGCCGCGGGCCCGTGACTGGGCGCGAGGCGGCCGGCGGCGGCGGCGGCACCAGCACCA CCATGTCGCGCTCAGTGCTGCAGCCCAGTCAGCAGAAGCTGGCGGAGAAGCTCACCATCCTCAACGACCGGGGCGTCGGCATGCTCACCCGCCTCTACAA CATCAAGAAGCAAGGACAAGTTTGGAAGGCATGTGGAGACCCCAAGGCAAAACCATCCTATCTTATCGACAAAAACCTGGAATCTGCTGTGAAATTCATA GTCAGAAAATTCCCTGCTGTAGAAACCCGCAACAACAATCAACAGCTTGCACAACTACAGAAAGAAAAATCAGAGATTCTGAAAAATCTGGCATTATATT ACTTCACATTTGTAGATGTTATGGAATTTAAGGACCATGTTTGTGAATTGCTGAATACTATTGACGTTTGCCAAGTCTTCTTTGATATTACTGTAAACTT TGATTTAACAAAGAACTACTTAGATTTAATTATAACCTATACAACACTAATGATACTGCTGTCTCGAATTGAAGAAAGGAAGGCAATCATTGGATTATAC AACTATGCCCATGAAATGACTCATGGAGCAAGTGACAGAGAATACCCACGCCTTGGCCAGATGATTGTGGATTATGAAAACCCTTTAAAGAAGATGATGG AAGAATTTGTACCCCATAGCAAGTCTCTTTCAGATGCACTAATTTCTCTTCAAATGGTATATCCTCGAAGGAATCTTTCAGCTGACCAGTGGAGAAATGC CCAGTTATTGAGCCTCATCAGTGCACCTAGTACAATGCTTAATCCAGCACAGTCCGACACTATGCCTTGTGAATACCTCTCTTTGGATGCAATGGAAAAG TGGATTATCTTTGGCTTTATTTTGTGCCATGGGATCCTAAATACTGACGCTACAGCACTGAACCTTTGGAAACTAGCTCTTCAAAGTAGCTCTTGCCTCT CTCTCTTTCGGGATGAAGTTTTCCACATTCACAAAGCTGCAGAAGACTTATTTGTAAACATACGAGGCTATAATAAACGTATTAATGACATAAGAGAATG CAAGGAGGCAGCCGTGTCACATGCTGGTTCAATGCACAGAGAAAGACGCAAGTTTTTAAGATCTGCACTGAAGGAATTGGCTACTGTCCTCTCTGATCAA CCTGGATTGCTAGGTCCCAAGGCACTTTTTGTTTTTATGGCATTATCCTTTGCCCGTGATGAAATCATCTGGCTACTTCGTCATGCAGATAACATGCCAA AGAAGAGTGCAGACGACTTTATAGATAAGCACATTGCTGAATTAATATTTTACATGGAAGAACTTAGAGCACATGTGAGGAAATACGGACCTGTAATGCA GAGGTATTACGTGCAGTACCTTTCTGGCTTTGATGCTGTTGTCCTCAATGAACTCGTGCAGAATCTTTCTGTTTGCCCTGAAGATGAATCAATCATCATG TCCTCTTTTGTTAACACTATGACTTCCCTAAGTGTAAAACAAGTTGAAGATGGGGAAGTATTTGATTTCAGAGGAATGAGATTAGATTGGTTTAGGTTAC AGGCATATACTAGTGTCTCAAAGGCTTCACTTGGCCTTGCAGATCACAGAGAACTTGGAAAGATGATGAATACAATAATTTTTCATACAAAAATGGTAGA TTCCTTGGTGGAAATGTTGGTGGAAACATCAGATCTCTCCATATTTTGTTTTTATAGTCGTGCTTTTGAGAAGATGTTTCAACAGTGTTTGGAGTTACCC TCTCAATCAAGATACTCAATTGCATTTCCACTACTTTGCACTCATTTTATGAGTTGCACGCATGAACTATGTCCAGAAGAGCGACATCATATTGGAGATC GCAGTCTTTCCTTATGTAATATGTTCCTAGATGAAATGGCCAAACAAGCTCGAAATCTCATCACTGATATTTGCACAGAACAGTGTACCCTTAGTGACCA GTTGCTACCCAAGCATTGTGCCAAAACTATCAGTCAAGCAGTGAATAAGAAATCAAAAAAGCAGACTGGTAAGAAAGGGGAACCTGAAAGGGAGAAACCA GGTGTTGAGAGCATGAGGAAAAACAGGCTGGTTGTGACCAACCTTGATAAATTGCACACTGCACTTTCTGAGTTATGCTTCTCTATAAATTATGTACCAA ACATGGTGGTATGGGAACATACCTTTACCCCACGAGAATATTTGACTTCTCATCTGGAAATACGCTTTACCAAGTCAATTGTTGGGATGACTATGTATAA TCAAGCCACACAGGAAATTGCAAAACCTTCAGAACTTCTAACAAGTGTAAGAGCATACATGACCGTACTCCAGTCAATAGAAAACTATGTGCAGATTGAT ATTACAAGAGTATTTAATAATGTGCTTCTTCAACAAACACAACATTTAGACAGTCATGGAGAGCCAACCATTACAAGTCTATACACAAATTGGTATTTGG AAACTTTGTTACGACAAGTCAGCAATGGCCATATAGCATATTTTCCTGCAATGAAAGCGTTTGTGAACTTACCTACAGAAAATGAATTAACATTCAATGC AGAGGAATATTCTGACATATCAGAAATGAGGTCATTATCAGAACTACTAGGCCCATATGGTATGAAGTTTCTAAGTGAAAGCCTTATGTGGCATATTTCA TCACAAGTTGCTGAACTTAAGAAACTTGTGGTGGAGAATGTTGATGTGTTAACACAAATGAGGACCAGCTTTGACAAACCAGACCAGATGGCTGCACTGT TTAAAAGATTATCATCTGTTGACAGTGTCTTGAAGAGGATGACAATAATTGGTGTAATTTTATCCTTCCGATCATTGGCACAAGAAGCACTTAGAGATGT CTTATCCTACCACATTCCTTTTCTTGTAAGTTCAATTGAAGATTTTAAGGATCACATTCCAAGGGAAACTGATATGAAGGTTGCAATGAATGTGTATGAG TTATCATCAGCTGCCGGATTACCTTGTGAGATTGATCCTGCATTGGTCGTAGCTCTTTCTTCACAAAAATCGGAAAACATTAGTCCAGAAGAAGAGTATA AAATTGCCTGCCTTCTCATGGTGTTTGTGGCAGTTTCTTTGCCAACACTGGCCAGTAATGTGATGTCTCAGTACAGCCCTGCTATAGAAGGGCATTGCAA CAACATACATTGCTTGGCCAAAGCCATCAACCAGATTGCTGCAGCTTTGTTTACAATTCACAAAGGAAGCATTGAAGACCGTCTTAAAGAATTTCTGGCG CTTGCATCCTCCAGTCTACTGAAAATTGGCCAGGAGACAGATAAAACTACAACAAGAAATAGAGAATCTGTTTATTTACTGCTAGATATGATTGTACAAG AATCTCCATTCCTTACAATGGATCTTTTGGAATCTTGTTTTCCTTATGTCTTGCTGAGAAATGCATACCATGCTGTCTACAAACAAAGTGTTACATCTTC TGCATAAAATTACCTACTTAATCAAGATAAGCACGCATTTTTGTTGCCTTGGTTTTACCTGTAGACTGTGGAACTATTTTACCTTAAGACCTGAAAAAGT TTTGTGGATTATAAATTTCTTTCATACGGTTGTATTTTCTGATCATTGGTTTCTTAATATGGTTGTACTACAGTATACTTGGTTGATTTAGGTTGCACAT TCACTGAATTCACTGAGATTATTCCTATAATTTTAAAGTATCATTTATTTGAAAAACATACATTATCAACATGTTTTTGATATTTGATAATGAAAAAAAT CTTTGCTTGTTTATTTCTGAAAAAGAACTGTATTTAGTGATTATTTTAGATAGTGATATTATAGTATTCATCTGTGTGTAAATTATTTCATATAGGGAAG AGTTCTGATCTGTACCTATGGTTCTTATTGAAAACAACATTGGATGTGCATTTCTGTGATGTTATGAATACATTTCTACTTTATTTTGAAACATTTGCCA AACTAAATACTGTAACACTGTATAACATTTAAAAATGTTAAAGAACTGCTTAGTATTAGAAGCAGATCATTTCCCAAAATTCTAAGAGCAGCAGCATATG TTGTTGCTTGTATAAAGCCTAGCGATAATTTTTAGACTAACTTCCATGGTGCCCTGTTGGCATTAGCACTACCATTGTACCCTGCTGTATAATAAACAAT CTTAGACATTTATCAACTGTTGATACAAATGTTAGTCCCTAACCACTTTTTATATATGTTTTAAATTTTTGAAATTCAAGTGTACCTGCCATAACATAAA ATAAACACTAGACTGTATCACACTTCGATTGA

refseq_NCKAP1.F1 (forward primer)
refseq_NCKAP1.R1 (reverse primer)
Single exon skipping

Frame 0

R*PRARPGLREAASFSAAGDAAAAAAAATPSGA AGEGGSLGAGAESAGGGGGRAAGGRQEAAGARAA AGEGAPRSPPRLALSPARDWGARAMGALS*GGG RGREGRRPERGCCEGRGPVTGREAAGGGGGTST TMSRSVLQPSQQKLAEKLTILNDRGVGMLTRLYN IKKQGQVWKACGDPKAKPSYLIDKNLESAVKFI VRKFPAVETRNNNQQLAQLQKEKSEILKNLALY YFTFVDVMEFKDHVCELLNTIDVCQVFFDITVNF DLTKNYLDLIITYTTLMILLSRIEERKAIIGLY NYAHEMTHGASDREYPRLGQMIVDYENPLKKMM EEFVPHSKSLSDALISLQMVYPRRNLSADQWRNA QLLSLISAPSTMLNPAQSDTMPCEYLSLDAMEK WIIFGFILCHGILNTDATALNLWKLALQSSSCL SLFRDEVFHIHKAAEDLFVNIRGYNKRINDIREC KEAAVSHAGSMHRERRKFLRSALKELATVLSDQ PGLLGPKALFVFMALSFARDEIIWLLRHADNMP KKSADDFIDKHIAELIFYMEELRAHVRKYGPVMQ RYYVQYLSGFDAVVLNELVQNLSVCPEDESIIM SSFVNTMTSLSVKQVEDGEVFDFRGMRLDWFRL QAYTSVSKASLGLADHRELGKMMNTIIFHTKMVD SLVEMLVETSDLSIFCFYSRAFEKMFQQCLELP SQSRYSIAFPLLCTHFMSCTHELCPEERHHIGD RSLSLCNMFLDEMAKQARNLITDICTEQCTLSDQ LLPKHCAKTISQAVNKKSKKQTGKKGEPEREKP GVESMRKNRLVVTNLDKLHTALSELCFSINYVP NMVVWEHTFTPREYLTSHLEIRFTKSIVGMTMYN QATQEIAKPSELLTSVRAYMTVLQSIENYVQID ITRVFNNVLLQQTQHLDSHGEPTITSLYTNWYL ETLLRQVSNGHIAYFPAMKAFVNLPTENELTFNA EEYSDISEMRSLSELLGPYGMKFLSESLMWHIS SQVAELKKLVVENVDVLTQMRTSFDKPDQMAAL FKRLSSVDSVLKRMTIIGVILSFRSLAQEALRDV LSYHIPFLVSSIEDFKDHIPRETDMKVAMNVYE LSSAAGLPCEIDPALVVALSSQKSENISPEEEY KIACLLMVFVAVSLPTLASNVMSQYSPAIEGHCN NIHCLAKAINQIAAALFTIHKGSIEDRLKEFLA LASSSLLKIGQETDKTTTRNRESVYLLLDMIVQ ESPFLTMDLLESCFPYVLLRNAYHAVYKQSVTSS A*NYLLNQDKHAFLLPWFYL*TVELFYLKT*KS FVDYKFLSYGCIF*SLVS*YGCTTVYLVDLGCT FTEFTEIIPIILKYHLFEKHTLSTCF*YLIMKKI FACLFLKKNCI**LF*IVIL*YSSVCKLFHIGK SSDLYLWFLLKTTLDVHFCDVMNTFLLYFETFA KLNTVTLYNI*KC*RTA*Y*KQIISQNSKSSSIC CCLYKA*R*FLD*LPWCPVGISTTIVPCCIINN LRHLSTVDTNVSP*PLFIYVLNF*NSSVPAIT* NKH*TVSHFD*

Frame 1

GDRGPVRGCGKRPRSQPPETPPPPPPPPHLVEQ PGKAARWGLGRRAPGVGAEGRRAEGRRLPGRGL LREKGLRGVRRGSLCRRRGIGARGPWARSPKAEV AGGRGGGPREAAAKAAGP*LGARRPAAAAAPAP PCRAQCCSPVSRSWRRSSPSSTTGASACSPAST TSRSKDKFGRHVETPRQNHPILSTKTWNLL*NS* SENSLL*KPATTINSLHNYRKKNQRF*KIWHYI TSHL*MLWNLRTMFVNC*ILLTFAKSSLILL*T LI*QRTT*I*L*PIQH**YCCLELKKGRQSLDYT TMPMK*LMEQVTENTHALAR*LWIMKTL*RR*W KNLYPIASLFQMH*FLFKWYILEGIFQLTSGEM PSY*ASSVHLVQCLIQHSPTLCLVNTSLWMQWKS GLSLALFCAMGS*ILTLQH*TFGN*LFKVALAS LSFGMKFSTFTKLQKTYL*TYEAIINVLMT*EN ARRQPCHMLVQCTEKDASF*DLH*RNWLLSSLIN LDC*VPRHFLFLWHYPLPVMKSSGYFVMQITCQ RRVQTTL*ISTLLN*YFTWKNLEHM*GNTDL*C RGITCSTFLALMLLSSMNSCRIFLFALKMNQSSC PLLLTL*LP*V*NKLKMGKYLISEE*D*IGLGY RHILVSQRLHLALQITENLER**IQ*FFIQKW* IPWWKCWWKHQISPYFVFIVVLLRRCFNSVWSYP LNQDTQLHFHYFALIL*VARMNYVQKSDIILEI AVFPYVICS*MKWPNKLEISSLIFAQNSVPLVT SCYPSIVPKLSVKQ*IRNQKSRLVRKGNLKGRNQ VLRA*GKTGWL*PTLINCTLHFLSYASL*IMYQ TWWYGNIPLPHENI*LLIWKYALPSQLLG*LCI IKPHRKLQNLQNF*QV*EHT*PYSSQ*KTMCRLI LQEYLIMCFFNKHNI*TVMESQPLQVYTQIGIW KLCYDKSAMAI*HIFLQ*KRL*TYLQKMN*HSM QRNILTYQK*GHYQNY*AHMV*SF*VKALCGIFH HKLLNLRNLWWRMLMC*HK*GPALTNQTRWLHC LKDYHLLTVS*RG*Q*LV*FYPSDHWHKKHLEM SYPTTFLFL*VQLKILRITFQGKLI*RLQ*MCMS YHQLPDYLVRLILHWS*LFLHKNRKTLVQKKSI KLPAFSWCLWQFLCQHWPVM*CLSTALL*KGIA TTYIAWPKPSTRLLQLCLQFTKEALKTVLKNFWR LHPPVY*KLARRQIKLQQEIENLFIYC*I*LYK NLHSLQWIFWNLVFLMSC*EMHTMLSTNKVLHL LHKITYLIKISTHFCCLGFTCRLWNYFTLRPEKV LWIINFFHTVVFSDHWFLNMVVLQYTWLI*VAH SLNSLRLFL*F*SIIYLKNIHYQHVFDI***KK SLLVYF*KRTVFSDYFR**YYSIHLCVNYFI*GR VLICTYGSY*KQHWMCISVML*IHFYFILKHLP N*IL*HCITFKNVKELLSIRSRSFPKILRAAAY VVACIKPSDNF*TNFHGALLALALPLYPAV**TI LDIYQLLIQMLVPNHFLYMF*IFEIQVYLP*HK INTRLYHTSI

Frame 2

VTEGPSGAAGSGLVLSRRRRRRRRRRRHT*WSS RGRRLVGGWGGERRGWGRKGGGRKAGGCRGAGC CGRRGSEESAAARSVAGAGLGREGHGRALLRRR SRAGGEEARERLLRRPRARDWARGGRRRRRHQHH HVALSAAAQSAEAGGEAHHPQRPGRRHAHPPLQ HQEARTSLEGMWRPQGKTILSYRQKPGICCEIH SQKIPCCRNPQQQSTACTTTERKIRDSEKSGIIL LHICRCYGI*GPCL*IAEYY*RLPSLL*YYCKL *FNKELLRFNYNLYNTNDTAVSN*RKEGNHWII QLCP*NDSWSK*QRIPTPWPDDCGL*KPFKEDDG RICTP*QVSFRCTNFSSNGISSKESFS*PVEKC PVIEPHQCT*YNA*SSTVRHYAL*IPLFGCNGK VDYLWLYFVPWDPKY*RYSTEPLETSSSK*LLPL SLSG*SFPHSQSCRRLICKHTRL**TY**HKRM QGGSRVTCWFNAQRKTQVFKICTEGIGYCPL*S TWIARSQGTFCFYGIILCP**NHLATSSCR*HAK EECRRLYR*AHC*INILHGRT*STCEEIRTCNA EVLRAVPFWL*CCCPQ*TRAESFCLP*R*INHH VLFC*HYDFPKCKTS*RWGSI*FQRNEIRLV*VT GIY*CLKGFTWPCRSQRTWKDDEYNNFSYKNGR FLGGNVGGNIRSLHILFL*SCF*EDVSTVFGVT LSIKILNCISTTLHSFYELHA*TMSRRATSYWRS QSFLM*YVPR*NGQTSSKSHH*YLHRTVYP**P VATQALCQNYQSSSE*EIKKADW*ERGT*KGET RC*EHEEKQAGCDQP**IAHCTF*VMLLYKLCTK HGGMGTYLYPTRIFDFSSGNTLYQVNCWDDYV* SSHTGNCKTFRTSNKCKSIHDRTPVNRKLCAD* YYKSI**CASSTNTTFRQSWRANHYKSIHKLVFG NFVTTSQQWPYSIFSCNESVCELTYRK*INIQC RGIF*HIRNEVIIRTTRPIWYEVSK*KPYVAYF ITSC*T*ETCGGEC*CVNTNEDQL*QTRPDGCTV *KIIIC*QCLEEDDNNWCNFILPIIGTRST*RC LILPHSFSCKFN*RF*GSHSKGN*YEGCNECV* VIISCRITL*D*SCIGRSSFFTKIGKH*SRRRV* NCLPSHGVCGSFFANTGQ*CDVSVQPCYRRALQ QHTLLGQSHQPDCCSFVYNSQRKH*RPS*RISG ACILQSTENWPGDR*NYNKK*RICLFTARYDCTR ISIPYNGSFGILFSLCLAEKCIPCCLQTKCYIF CIKLPT*SR*ARIFVALVLPVDCGTILP*DLKK FCGL*ISFIRLYFLIIGFLIWLYYSILG*FRLHI H*IH*DYSYNFKVSFI*KTYIINMFLIFDNEKN LCLFISEKELYLVIILDSDIIVFICV*IISYRE EF*SVPMVLIENNIGCAFL*CYEYISTLF*NICQ TKYCNTV*HLKMLKNCLVLEADHFPKF*EQQHM LLLV*SLAIIFRLTSMVPCWH*HYHCTLLYNKQ S*TFINC*YKC*SLTTFYICFKFLKFKCTCHNIK *TLDCITLRL