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Sequences

Sequence NM_130473

ACCGCGGCCGCGCGCCCCCCTCTGCCCCCGCTGCCCAGGATTGGTAGGCTCCACCGCTCGGCAGCCGGCTTCCCTGCTCGGACGCCGAGCACCGCCAAAG CGCACTTCGATTTTCAGAATTCCTCCTGGGAATGCTGACTCCTTGCTTGGTGCCCTGATGCTTCTCTGAGATAAACTGATGAATTGGAACCATGGTGCAA AAGAAGAAGTTCTGTCCTCGGTTACTTGACTATCTAGTGATCGTAGGGGCCAGGCACCCGAGCAGTGATAGCGTGGCCCAGACTCCTGAATTGCTACGGC GATACCCCTTGGAGGATCACACTGAGTTTCCCCTGCCCCCAGATGTAGTGTTCTTCTGCCAGCCCGAGGGCTGCCTGAGCGTGCGGCAGCGGCGCATGAG CCTTCGGGATGATACCTCTTTTGTCTTCACCCTCACTGACAAGGACACTGGAGTCACGCGATATGGCATCTGTGTTAACTTCTACCGCTCCTTCCAAAAG CGAATCTCTAAGGAGAAGGGGGAAGGTGGGGCAGGGTCCCGTGGGAAGGAAGGAACCCATGCCACCTGTGCCTCAGAAGAGGGTGGCACTGAGAGCTCAG AGAGTGGCTCATCCCTGCAGCCTCTCAGTGCTGACTCTACCCCTGATGTGAACCAGTCTCCTCGGGGCAAACGCCGGGCCAAGGCGGGGAGCCGCTCCCG CAACAGTACTCTCACGTCCCTGTGCGTGCTCAGCCACTACCCTTTCTTCTCCACCTTCCGAGAGTGTTTGTATACTCTCAAGCGCCTGGTGGACTGCTGT AGTGAGCGCCTTCTGGGCAAGAAACTGGGCATCCCTCGAGGCGTACAAAGGGACACCATGTGGCGGATCTTTACTGGATCGCTGCTGGTAGAGGAGAAGT CAAGTGCCCTTCTGCATGACCTTCGAGAGATTGAGGCCTGGATCTATCGATTGCTGCGCTCCCCAGTACCCGTCTCTGGGCAGAAGCGAGTAGACATCGA GGTCCTACCCCAAGAGCTCCAGCCAGCTCTGACCTTTGCTCTTCCAGACCCATCTCGATTCACCCTAGTGGATTTCCCACTGCACCTTCCCTTGGAACTT CTAGGTGTGGACGCCTGTCTCCAGGTGCTAACCTGCATTCTGTTAGAGCACAAGGTGGTGCTACAGTCCCGAGACTACAATGCACTCTCCATGTCTGTGA TGGCATTCGTGGCAATGATCTACCCACTGGAGTATATGTTTCCTGTCATCCCGCTGCTACCCACCTGCATGGCATCAGCAGAGCAGCTGCTGTTGGCTCC AACCCCGTACATCATTGGGGTTCCTGCCAGCTTCTTCCTCTACAAACTGGACTTCAAAATGCCTGATGATGTATGGCTAGTGGATCTGGACAGCAATAGG GTGATTGCCCCCACCAATGCAGAAGTGCTGCCTATCCTGCCAGAACCAGAATCACTAGAGCTGAAAAAGCATTTAAAGCAGGCCTTGGCCAGCATGAGTC TCAACACCCAGCCCATCCTCAATCTGGAGAAATTTCATGAGGGCCAGGAGATCCCCCTTCTCTTGGGAAGGCCTTCTAATGACCTGCAGTCCACACCGTC CACTGAATTCAACCCACTCATCTATGGCAATGATGTGGATTCTGTGGATGTTGCAACCAGGGTTGCCATGGTACGGTTCTTCAATTCCGCCAACGTGCTG CAGGGATTTCAGATGCACACGCGTACCCTGCGCCTCTTTCCTCGGCCTGTGGTAGCTTTTCAAGCTGGCTCCTTTCTAGCCTCACGTCCCCGGCAGACTC CTTTTGCCGAGAAATTGGCCAGGACTCAGGCTGTGGAGTACTTTGGGGAATGGATCCTTAACCCCACCAACTATGCCTTTCAGCGAATTCACAACAATAT GTTTGATCCAGCCCTGATTGGTGACAAGCCAAAGTGGTATGCTCATCAGCTGCAGCCTATCCACTATCGCGTCTATGACAGCAATTCCCAGCTGGCTGAG GCCCTGAGTGTACCACCAGAGCGGGACTCTGACTCCGAACCTACTGATGATAGTGGCAGTGATAGTATGGATTATGACGATTCAAGCTCTTCTTACTCCT CCCTTGGTGACTTTGTCAGTGAAATGATGAAATGTGACATTAATGGTGATACTCCCAATGTGGACCCTCTGACACATGCAGCACTGGGGGATGCCAGCGA GGTGGAGATTGACGAGCTGCAGAATCAGAAGGAAGCAGAAGAGCCTGGCCCAGACAGTGAGAACTCTCAGGAAAACCCCCCACTGCGCTCCAGCTCTAGC ACCACAGCCAGCAGCAGCCCCAGCACTGTCATCCACGGAGCCAACTCTGAACCTGCTGACTCTACGGAGATGGATGATAAGGCAGCAGTAGGCGTCTCCA AGCCCCTCCCTTCCGTGCCTCCCAGCATTGGCAAATCGAACGTGGACAGACGTCAGGCAGAAATTGGAGAGGGGTCAGTGCGCCGGCGAATCTATGACAA TCCATACTTCGAGCCCCAATATGGCTTTCCCCCTGAGGAAGATGAGGATGAGCAGGGGGAAAGTTACACTCCCCGATTCAGCCAACATGTCAGTGGCAAT CGGGCTCAAAAGCTGCTGCGGCCCAACAGCTTGAGACTGGCAAGTGACTCAGATGCAGAGTCAGACTCTCGGGCAAGCTCTCCCAACTCCACCGTCTCCA ACACCAGCACCGAGGGCTTCGGGGGCATCATGTCTTTTGCCAGCAGCCTCTATCGGAACCACAGTACCAGCTTCAGTCTTTCAAACCTCACACTGCCCAC CAAAGGTGCCCGAGAGAAGGCCACGCCCTTCCCCAGTCTGAAAGTATTTGGGCTAAATACTCTAATGGAGATTGTTACTGAAGCCGGCCCCGGGAGTGGT GAAGGAAACAGGAGGGCGTTAGTGGATCAGAAGTCATCTGTCATTAAACACAGCCCAACAGTGAAAAGAGAACCTCCATCACCCCAGGGTCGATCCAGCA ATTCTAGTGAGAACCAGCAGTTCCTGAAGGAGGTGGTGCACAGCGTGCTGGACGGCCAGGGAGTTGGCTGGCTCAACATGAAAAAGGTGCGCCGGCTGCT GGAGAGCGAGCAGCTGCGAGTCTTTGTCCTGAGCAAGCTGAACCGCATGGTGCAGTCAGAGGACGATGCCCGGCAGGACATCATCCCGGATGTGGAGATC AGTCGGAAGGTGTACAAGGGAATGTTAGACCTCCTCAAGTGTACAGTCCTCAGCTTGGAGCAGTCCTATGCCCACGCGGGTCTGGGTGGCATGGCCAGCA TCTTTGGGCTTTTGGAGATTGCCCAGACCCACTACTATAGTAAAGAACCAGACAAGCGGAAGAGAAGTCCAACAGAAAGTGTAAATACCCCAGTTGGCAA GGATCCTGGCCTAGCTGGGCGGGGGGACCCAAAGGCTATGGCACAACTGAGAGTTCCACAACTGGGACCTCGGGCACCAAGTGCCACAGGAAAGGGTCCT AAGGAACTGGACACCAGAAGTTTAAAGGAAGAAAATTTTATAGCATCTATTGGGCCTGAAGTAATCAAACCTGTCTTTGACCTTGGTGAGACAGAGGAGA AAAAGTCCCAGATCAGCGCAGACAGTGGTGTGAGCCTGACGTCTAGTTCCCAGAGGACTGATCAAGACTCTGTCATCGGCGTGAGTCCAGCTGTTATGAT CCGCAGCTCAAGTCAGGATTCTGAAGTTAGCACCGTGGTGAGTAATAGCTCTGGAGAGACCCTTGGAGCTGACAGTGACTTGAGCAGCAATGCAGGTGAT GGACCAGGTGGCGAGGGCAGTGTTCACCTGGCAAGCTCTCGGGGCACTTTGTCTGATAGTGAAATTGAGACCAACTCTGCCACAAGCACCATCTTTGGTA AAGCCCACAGCTTGAAGCCAAGCATAAAGGAGAAGCTGGCAGGCAGCCCCATTCGTACTTCTGAAGATGTGAGCCAGCGAGTCTATCTCTATGAGGGACT CCTAGGCAAAGAGCGTTCTACTTTATGGGACCAAATGCAATTCTGGGAAGATGCCTTCTTAGATGCTGTGATGTTGGAGAGAGAAGGGATGGGTATGGAC CAGGGTCCCCAGGAAATGATCGACAGGTACCTGTCCCTTGGAGAACATGACCGGAAGCGCCTGGAAGATGATGAAGATCGCTTGTTGGCCACACTTCTGC ACAACCTCATCTCCTACATGCTGCTGATGAAGGTAAATAAGAATGACATCCGCAAGAAGGTGAGGCGCCTAATGGGAAAGTCGCACATTGGGCTTGTGTA CAGCCAGCAAATCAATGAGGTGCTTGATCAGCTGGCGAACCTGAATGGACGCGATCTCTCTATCTGGTCCAGTGGCAGCCGGCACATGAAGAAGCAGACA TTTGTGGTACATGCAGGGACAGATACAAACGGAGATATCTTTTTCATGGAGGTGTGCGATGACTGTGTGGTGTTGCGTAGTAACATCGGAACAGTGTATG AGCGCTGGTGGTACGAGAAGCTCATCAACATGACCTACTGTCCCAAGACGAAGGTGTTGTGCTTGTGGCGTAGAAATGGCTCTGAGACCCAGCTCAACAA GTTCTATACTAAAAAGTGTCGGGAGCTGTACTACTGTGTGAAGGACAGCATGGAGCGCGCTGCCGCCCGACAGCAAAGCATCAAACCCGGACCTGAATTG GGTGGCGAGTTCCCTGTGCAGGACCTGAAGACTGGTGAGGGTGGCCTGCTGCAGGTGACCCTGGAAGGGATCAACCTCAAATTCATGCACAATCAGGTTT TCATAGAGCTGAATCACATTAAAAAGTGCAATACAGTTCGAGGCGTCTTTGTCCTGGAGGAATTTGTTCCTGAAATTAAAGAAGTGGTGAGCCACAAGTA CAAGACACCAATGGCCCACGAAATCTGCTACTCCGTATTATGTCTCTTCTCGTACGTGGCTGCAGTTCATAGCAGTGAGGAAGATCTCAGAACCCCGCCC CGGCCTGTCTCTAGCTGATGGAGAGGGGCTACGCAGCTGCCCCAGCCCAGGGCACGCCCCTGGCCCCTTGCTGTTCCCAAGTGCACGATGCTGCTGTGAC TGAGGAGTGGATGATGCTCGTGTGTCCTCTGCAAGCCCCCTGCTGTGGCTTGGTTGGTTACCGGTTATGTGTCCCTCTGAGTGTGTCTTGAGCGTGTCCA CCTTCTCCCTCTCCACTCCCAGAAGACCAAACTGCCTTCCCCTCAGGGCTCAAGAATGTGTACAGTCTGTGGGGCCGGTGTGAACCCACTATTTTGTGTC CTTGAGACATTTGTGTTGTGGTTCCTTGTCCTTGTCCCTGGCGTTATAACTGTCCACTGCAAGAGTCTGGCTCTCCCTTCTCTGTGACCCGGCATGACTG GGCGCCTGGAGCAGTTTCACTCTGTGAGGAGTGAGGGAACCCTGGGGCTCACCCTCTCAGAGGAAGGGCACAGAGAGGAAGGGAAGAATTGGGGGGCAGC CGGAGTGAGTGGCAGCCTCCCTGCTTCCTTCTGCATTCCCAAGCCGGCAGCTACTGCCCAGGGCCCGCAGTGTTGGCTGCTGCCTGCCACAGCCTCTGTG ACTGCAGTGGAGCGGCGAATTCCCTGTGGCCTGCCATGCCTTCGGCATCAGAGGATGGAGTGGTCGAGGCTAGTGGAGTCCCAGGGACCGCTGGCTGCTC TGCCTGAGCATCAGGGAGGGGGCAGGAAAGACCAAGCTGGGTTTGCACATCTGTCTGCAGGCTGTCTCTCCAGGCACGGGGTGTCAGGAGGGAGAGACAG CCTGGGTATGGGCAAGAAATGACTGTAAATATTTCAGCCCCACATTATTTATAGAAAATGTACAGTTGTGTGAATGTGAAATAAATGTCCTCAACTCCC

refseq_MADD.F8 (forward primer)
refseq_MADD.R8 (reverse primer)
Single exon skipping

Frame 0

TAAARPPLPPLPRIGRLHRSAAGFPARTPSTAK AHFDFQNSSWEC*LLAWCPDASLR*TDELEPWCK RRSSVLGYLTI**S*GPGTRAVIAWPRLLNCYG DTPWRITLSFPCPQM*CSSASPRAA*ACGSGA* AFGMIPLLSSPSLTRTLESRDMASVLTSTAPSKS ESLRRRGKVGQGPVGRKEPMPPVPQKRVALRAQ RVAHPCSLSVLTLPLM*TSLLGANAGPRRGAAP ATVLSRPCACSATTLSSPPSESVCILSSAWWTAV VSAFWARNWASLEAYKGTPCGGSLLDRCW*RRS QVPFCMTFERLRPGSIDCCAPQYPSLGRSE*TS RSYPKSSSQL*PLLFQTHLDSP*WISHCTFPWNF *VWTPVSRC*PAFC*STRWCYSPETTMHSPCL* WHSWQ*STHWSICFLSSRCYPPAWHQQSSCCWL QPRTSLGFLPASSSTNWTSKCLMMYG*WIWTAIG *LPPPMQKCCLSCQNQNH*S*KSI*SRPWPA*V STPSPSSIWRNFMRARRSPFSWEGLLMTCSPHR PLNSTHSSMAMMWILWMLQPGLPWYGSSIPPTCC RDFRCTRVPCASFLGLW*LFKLAPF*PHVPGRL LLPRNWPGLRLWSTLGNGSLTPPTMPFSEFTTI CLIQP*LVTSQSGMLISCSLSTIASMTAIPSWLR P*VYHQSGTLTPNLLMIVAVIVWIMTIQALLTP PLVTLSVK**NVTLMVILPMWTL*HMQHWGMPA RWRLTSCRIRRKQKSLAQTVRTLRKTPHCAPALA PQPAAAPALSSTEPTLNLLTLRRWMIRQQ*ASP SPSLPCLPALANRTWTDVRQKLERGQCAGESMT IHTSSPNMAFPLRKMRMSRGKVTLPDSANMSVAI GLKSCCGPTA*DWQVTQMQSQTLGQALPTPPSP TPAPRASGASCLLPAASIGTTVPASVFQTSHCP PKVPERRPRPSPV*KYLG*IL*WRLLLKPAPGVV KETGGR*WIRSHLSLNTAQQ*KENLHHPRVDPA ILVRTSSS*RRWCTACWTARELAGST*KRCAGC WRASSCESLS*AS*TAWCSQRTMPGRTSSRMWRS VGRCTREC*TSSSVQSSAWSSPMPTRVWVAWPA SLGFWRLPRPTTIVKNQTSGREVQQKV*IPQLA RILA*LGGGTQRLWHN*EFHNWDLGHQVPQERVL RNWTPEV*RKKIL*HLLGLK*SNLSLTLVRQRR KSPRSAQTVV*A*RLVPRGLIKTLSSA*VQLL* SAAQVRILKLAPW*VIALERPLELTVT*AAMQVM DQVARAVFTWQALGALCLIVKLRPTLPQAPSLV KPTA*SQA*RRSWQAAPFVLLKM*ASESISMRD S*AKSVLLYGTKCNSGKMPS*ML*CWREKGWVWT RVPRK*STGTCPLENMTGSAWKMMKIACWPHFC TTSSPTCC**R*IRMTSARR*GA*WESRTLGLC TASKSMRCLISWRT*MDAISLSGPVAAGT*RSRH LWYMQGQIQTEISFSWRCAMTVWCCVVTSEQCM SAGGTRSSST*PTVPRRRCCACGVEMALRPSST SSILKSVGSCTTV*RTAWSALPPDSKASNPDLNW VASSLCRT*RLVRVACCR*PWKGSTSNSCTIRF S*S*ITLKSAIQFEASLSWRNLFLKLKKW*ATS TRHQWPTKSATPYYVSSRTWLQFIAVRKISEPRP GLSLADGEGLRSCPSPGHAPGPLLFPSARCCCD *GVDDARVSSASPLLWLGWLPVMCPSECVLSVS TFSLSTPRRPNCLPLRAQECVQSVGPV*THYFVS LRHLCCGSLSLSLAL*LSTARVWLSLLCDPA*L GAWSSFTL*GVREPWGSPSQRKGTERKGRIGGQ PE*VAASLLPSAFPSRQLLPRARSVGCCLPQPL* LQWSGEFPVACHAFGIRGWSGRG*WSPRDRWLL CLSIREGAGKTKLGLHICLQAVSPGTGCQEGET AWVWARNDCKYFSPTLFIENVQLCECEINVLNS

Frame 1

PRPRAPLCPRCPGLVGSTARQPASLLGRRAPPK RTSIFRIPPGNADSLLGALMLL*DKLMNWNHGA KEEVLSSVT*LSSDRRGQAPEQ**RGPDS*IATA IPLGGSH*VSPAPRCSVLLPARGLPERAAAAHE PSG*YLFCLHPH*QGHWSHAIWHLC*LLPLLPK ANL*GEGGRWGRVPWEGRNPCHLCLRRGWH*ELR EWLIPAASQC*LYP*CEPVSSGQTPGQGGEPLP QQYSHVPVRAQPLPFLLHLPRVFVYSQAPGGLL **APSGQETGHPSRRTKGHHVADLYWIAAGRGEV KCPSA*PSRD*GLDLSIAALPSTRLWAEASRHR GPTPRAPASSDLCSSRPISIHPSGFPTAPSLGT SRCGRLSPGANLHSVRAQGGATVPRLQCTLHVCD GIRGNDLPTGVYVSCHPAATHLHGISRAAAVGS NPVHHWGSCQLLPLQTGLQNA**CMASGSGQQ* GDCPHQCRSAAYPARTRITRAEKAFKAGLGQHES QHPAHPQSGEIS*GPGDPPSLGKAF**PAVHTV H*IQPTHLWQ*CGFCGCCNQGCHGTVLQFRQRA AGISDAHAYPAPLSSACGSFSSWLLSSLTSPADS FCREIGQDSGCGVLWGMDP*PHQLCLSANSQQY V*SSPDW*QAKVVCSSAAAYPLSRL*QQFPAG* GPECTTRAGL*LRTY***WQ**YGL*RFKLFLLL PW*LCQ*NDEM*H*W*YSQCGPSDTCSTGGCQR GGD*RAAESEGSRRAWPRQ*ELSGKPPTALQL* HHSQQQPQHCHPRSQL*TC*LYGDG**GSSRRLQ APPFRASQHWQIERGQTSGRNWRGVSAPANL*Q SILRAPIWLSP*GR*G*AGGKLHSPIQPTCQWQ SGSKAAAAQQLETGK*LRCRVRLSGKLSQLHRLQ HQHRGLRGHHVFCQQPLSEPQYQLQSFKPHTAH QRCPREGHALPQSESIWAKYSNGDCY*SRPREW *RKQEGVSGSEVICH*TQPNSEKRTSITPGSIQQ F**EPAVPEGGGAQRAGRPGSWLAQHEKGAPAA GERAAASLCPEQAEPHGAVRGRCPAGHHPGCGD QSEGVQGNVRPPQVYSPQLGAVLCPRGSGWHGQH LWAFGDCPDPLL**RTRQAEEKSNRKCKYPSWQ GSWPSWAGGPKGYGTTESSTTGTSGTKCHRKGS *GTGHQKFKGRKFYSIYWA*SNQTCL*PW*DRGE KVPDQRRQWCEPDV*FPED*SRLCHRRESSCYD PQLKSGF*S*HRGE**LWRDPWS*Q*LEQQCR* WTRWRGQCSPGKLSGHFV***N*DQLCHKHHLW* SPQLEAKHKGEAGRQPHSYF*RCEPASLSL*GT PRQRAFYFMGPNAILGRCLLRCCDVGERRDGYG PGSPGNDRQVPVPWRT*PEAPGR**RSLVGHTSA QPHLLHAADEGK*E*HPQEGEAPNGKVAHWACV QPANQ*GA*SAGEPEWTRSLYLVQWQPAHEEAD ICGTCRDRYKRRYLFHGGVR*LCGVA**HRNSV* ALVVREAHQHDLLSQDEGVVLVA*KWL*DPAQQ VLY*KVSGAVLLCEGQHGARCRPTAKHQTRT*I GWRVPCAGPEDW*GWPAAGDPGRDQPQIHAQSGF HRAESH*KVQYSSRRLCPGGICS*N*RSGEPQV QDTNGPRNLLLRIMSLLVRGCSS*Q*GRSQNPA PACL*LMERGYAAAPAQGTPLAPCCSQVHDAAVT EEWMMLVCPLQAPCCGLVGYRLCVPLSVS*ACP PSPSPLPEDQTAFPSGLKNVYSLWGRCEPTILC P*DICVVVPCPCPWRYNCPLQESGSPFSVTRHDW APGAVSLCEE*GNPGAHPLRGRAQRGREELGGS RSEWQPPCFLLHSQAGSYCPGPAVLAAACHSLC DCSGAANSLWPAMPSASEDGVVEASGVPGTAGCS A*ASGRGQERPSWVCTSVCRLSLQARGVRRERQ PGYGQEMTVNISAPHYL*KMYSCVNVK*MSSTP

Frame 2

RGRAPPSAPAAQDW*APPLGSRLPCSDAEHRQS ALRFSEFLLGMLTPCLVP*CFSEIN**IGTMVQ KKKFCPRLLDYLVIVGARHPSSDSVAQTPELLR RYPLEDHTEFPLPPDVVFFCQPEGCLSVRQRRMS LRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQK RISKEKGEGGAGSRGKEGTHATCASEEGGTESS ESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSR NSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCC SERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEK SSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIE VLPQELQPALTFALPDPSRFTLVDFPLHLPLEL LGVDACLQVLTCILLEHKVVLQSRDYNALSMSV MAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAP TPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNR VIAPTNAEVLPILPEPESLELKKHLKQALASMS LNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPS TEFNPLIYGNDVDSVDVATRVAMVRFFNSANVL QGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQT PFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNM FDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAE ALSVPPERDSDSEPTDDSGSDSMDYDDSSSSYS SLGDFVSEMMKCDINGDTPNVDPLTHAALGDASE VEIDELQNQKEAEEPGPDSENSQENPPLRSSSS TTASSSPSTVIHGANSEPADSTEMDDKAAVGVS KPLPSVPPSIGKSNVDRRQAEIGEGSVRRRIYDN PYFEPQYGFPPEEDEDEQGESYTPRFSQHVSGN RAQKLLRPNSLRLASDSDAESDSRASSPNSTVS NTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPT KGAREKATPFPSLKVFGLNTLMEIVTEAGPGSG EGNRRALVDQKSSVIKHSPTVKREPPSPQGRSS NSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLL ESEQLRVFVLSKLNRMVQSEDDARQDIIPDVEI SRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMAS IFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGK DPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGP KELDTRSLKEENFIASIGPEVIKPVFDLGETEE KKSQISADSGVSLTSSSQRTDQDSVIGVSPAVMI RSSSQDSEVSTVVSNSSGETLGADSDLSSNAGD GPGGEGSVHLASSRGTLSDSEIETNSATSTIFG KAHSLKPSIKEKLAGSPIRTSEDVSQRVYLYEGL LGKERSTLWDQMQFWEDAFLDAVMLEREGMGMD QGPQEMIDRYLSLGEHDRKRLEDDEDRLLATLL HNLISYMLLMKVNKNDIRKKVRRLMGKSHIGLVY SQQINEVLDQLANLNGRDLSIWSSGSRHMKKQT FVVHAGTDTNGDIFFMEVCDDCVVLRSNIGTVY ERWWYEKLINMTYCPKTKVLCLWRRNGSETQLNK FYTKKCRELYYCVKDSMERAAARQQSIKPGPEL GGEFPVQDLKTGEGGLLQVTLEGINLKFMHNQV FIELNHIKKCNTVRGVFVLEEFVPEIKEVVSHKY KTPMAHEICYSVLCLFSYVAAVHSSEEDLRTPP RPVSS*WRGATQLPQPRARPWPLAVPKCTMLL* LRSG*CSCVLCKPPAVAWLVTGYVSL*VCLERVH LLPLHSQKTKLPSPQGSRMCTVCGAGVNPLFCV LETFVLWFLVLVPGVITVHCKSLALPSL*PGMT GRLEQFHSVRSEGTLGLTLSEEGHREEGKNWGAA GVSGSLPASFCIPKPAATAQGPQCWLLPATASV TAVERRIPCGLPCLRHQRMEWSRLVESQGPLAA LPEHQGGGRKDQAGFAHLSAGCLSRHGVSGGRDS LGMGKK*L*IFQPHIIYRKCTVV*M*NKCPQL

Sequence NM_130476

AGTGCAGACGCGGAACTGGCGCCCGCTCGGAGCGGCGCCGCGCTGGGGAGCGACTGACGCCCCGCTGCCGGGGGACGTCGGGCTGGGCCTGGCCAGTCCC CCAGAGCTTGGGAGACTTCGATTTTCAGAATTCCTCCTGGGAATGCTGACTCCTTGCTTGGTGCCCTGATGCTTCTCTGAGATAAACTGATGAATTGGAA CCATGGTGCAAAAGAAGAAGTTCTGTCCTCGGTTACTTGACTATCTAGTGATCGTAGGGGCCAGGCACCCGAGCAGTGATAGCGTGGCCCAGACTCCTGA ATTGCTACGGCGATACCCCTTGGAGGATCACACTGAGTTTCCCCTGCCCCCAGATGTAGTGTTCTTCTGCCAGCCCGAGGGCTGCCTGAGCGTGCGGCAG CGGCGCATGAGCCTTCGGGATGATACCTCTTTTGTCTTCACCCTCACTGACAAGGACACTGGAGTCACGCGATATGGCATCTGTGTTAACTTCTACCGCT CCTTCCAAAAGCGAATCTCTAAGGAGAAGGGGGAAGGTGGGGCAGGGTCCCGTGGGAAGGAAGGAACCCATGCCACCTGTGCCTCAGAAGAGGGTGGCAC TGAGAGCTCAGAGAGTGGCTCATCCCTGCAGCCTCTCAGTGCTGACTCTACCCCTGATGTGAACCAGTCTCCTCGGGGCAAACGCCGGGCCAAGGCGGGG AGCCGCTCCCGCAACAGTACTCTCACGTCCCTGTGCGTGCTCAGCCACTACCCTTTCTTCTCCACCTTCCGAGAGTGTTTGTATACTCTCAAGCGCCTGG TGGACTGCTGTAGTGAGCGCCTTCTGGGCAAGAAACTGGGCATCCCTCGAGGCGTACAAAGGGACACCATGTGGCGGATCTTTACTGGATCGCTGCTGGT AGAGGAGAAGTCAAGTGCCCTTCTGCATGACCTTCGAGAGATTGAGGCCTGGATCTATCGATTGCTGCGCTCCCCAGTACCCGTCTCTGGGCAGAAGCGA GTAGACATCGAGGTCCTACCCCAAGAGCTCCAGCCAGCTCTGACCTTTGCTCTTCCAGACCCATCTCGATTCACCCTAGTGGATTTCCCACTGCACCTTC CCTTGGAACTTCTAGGTGTGGACGCCTGTCTCCAGGTGCTAACCTGCATTCTGTTAGAGCACAAGGTGGTGCTACAGTCCCGAGACTACAATGCACTCTC CATGTCTGTGATGGCATTCGTGGCAATGATCTACCCACTGGAGTATATGTTTCCTGTCATCCCGCTGCTACCCACCTGCATGGCATCAGCAGAGCAGCTG CTGTTGGCTCCAACCCCGTACATCATTGGGGTTCCTGCCAGCTTCTTCCTCTACAAACTGGACTTCAAAATGCCTGATGATGTATGGCTAGTGGATCTGG ACAGCAATAGGGTGATTGCCCCCACCAATGCAGAAGTGCTGCCTATCCTGCCAGAACCAGAATCACTAGAGCTGAAAAAGCATTTAAAGCAGGCCTTGGC CAGCATGAGTCTCAACACCCAGCCCATCCTCAATCTGGAGAAATTTCATGAGGGCCAGGAGATCCCCCTTCTCTTGGGAAGGCCTTCTAATGACCTGCAG TCCACACCGTCCACTGAATTCAACCCACTCATCTATGGCAATGATGTGGATTCTGTGGATGTTGCAACCAGGGTTGCCATGGTACGGTTCTTCAATTCCG CCAACGTGCTGCAGGGATTTCAGATGCACACGCGTACCCTGCGCCTCTTTCCTCGGCCTGTGGTAGCTTTTCAAGCTGGCTCCTTTCTAGCCTCACGTCC CCGGCAGACTCCTTTTGCCGAGAAATTGGCCAGGACTCAGGCTGTGGAGTACTTTGGGGAATGGATCCTTAACCCCACCAACTATGCCTTTCAGCGAATT CACAACAATATGTTTGATCCAGCCCTGATTGGTGACAAGCCAAAGTGGTATGCTCATCAGCTGCAGCCTATCCACTATCGCGTCTATGACAGCAATTCCC AGCTGGCTGAGGCCCTGAGTGTACCACCAGAGCGGGACTCTGACTCCGAACCTACTGATGATAGTGGCAGTGATAGTATGGATTATGACGATTCAAGCTC TTCTTACTCCTCCCTTGGTGACTTTGTCAGTGAAATGATGAAATGTGACATTAATGGTGATACTCCCAATGTGGACCCTCTGACACATGCAGCACTGGGG GATGCCAGCGAGGTGGAGATTGACGAGCTGCAGAATCAGAAGGAAGCAGAAGAGCCTGGCCCAGACAGTGAGAACTCTCAGGAAAACCCCCCACTGCGCT CCAGCTCTAGCACCACAGCCAGCAGCAGCCCCAGCACTGTCATCCACGGAGCCAACTCTGAACCTGCTGACTCTACGGAGATGGATGATAAGGCAGCAGT AGGCGTCTCCAAGCCCCTCCCTTCCGTGCCTCCCAGCATTGGCAAATCGAACGTGGACAGACGTCAGGCAGAAATTGGAGAGGGGTCAGTGCGCCGGCGA ATCTATGACAATCCATACTTCGAGCCCCAATATGGCTTTCCCCCTGAGGAAGATGAGGATGAGCAGGGGGAAAGTTACACTCCCCGATTCAGCCAACATG TCAGTGGCAATCGGGCTCAAAAGCTGCTGCGGCCCAACAGCTTGAGACTGGCAAGTGACTCAGATGCAGAGTCAGACTCTCGGGCAAGCTCTCCCAACTC CACCGTCTCCAACACCAGCACCGAGGGCTTCGGGGGCATCATGTCTTTTGCCAGCAGCCTCTATCGGAACCACAGTACCAGCTTCAGTCTTTCAAACCTC ACACTGCCCACCAAAGGTGCCCGAGAGAAGGCCACGCCCTTCCCCAGTCTGAAAGGAAACAGGAGGGCGTTAGTGGATCAGAAGTCATCTGTCATTAAAC ACAGCCCAACAGTGAAAAGAGAACCTCCATCACCCCAGGGTCGATCCAGCAATTCTAGTGAGAACCAGCAGTTCCTGAAGGAGGTGGTGCACAGCGTGCT GGACGGCCAGGGAGTTGGCTGGCTCAACATGAAAAAGGTGCGCCGGCTGCTGGAGAGCGAGCAGCTGCGAGTCTTTGTCCTGAGCAAGCTGAACCGCATG GTGCAGTCAGAGGACGATGCCCGGCAGGACATCATCCCGGATGTGGAGATCAGTCGGAAGGTGTACAAGGGAATGTTAGACCTCCTCAAGTGTACAGTCC TCAGCTTGGAGCAGTCCTATGCCCACGCGGGTCTGGGTGGCATGGCCAGCATCTTTGGGCTTTTGGAGATTGCCCAGACCCACTACTATAGTAAAGAACC AGACAAGCGGAAGAGAAGTCCAACAGAAAGTGTAAATACCCCAGTTGGCAAGGATCCTGGCCTAGCTGGGCGGGGGGACCCAAAGGCTATGGCACAACTG AGAGTTCCACAACTGGGACCTCGGGCACCAAGTGCCACAGGAAAGGGTCCTAAGGAACTGGACACCAGAAGTTTAAAGGAAGAAAATTTTATAGCATCTA TTGGGCCTGAAGTAATCAAACCTGTCTTTGACCTTGGTGAGACAGAGGAGAAAAAGTCCCAGATCAGCGCAGACAGTGGTGTGAGCCTGACGTCTAGTTC CCAGAGGACTGATCAAGACTCTGTCATCGGCGTGAGTCCAGCTGTTATGATCCGCAGCTCAAGTCAGGATTCTGAAGTTAGCACCGTGGTGAGTAATAGC TCTGGAGAGACCCTTGGAGCTGACAGTGACTTGAGCAGCAATGCAGGTGATGGACCAGGTGGCGAGGGCAGTGTTCACCTGGCAAGCTCTCGGGGCACTT TGTCTGATAGTGAAATTGAGACCAACTCTGCCACAAGCACCATCTTTGGTAAAGCCCACAGCTTGAAGCCAAGCATAAAGGAGAAGCTGGCAGGCAGCCC CATTCGTACTTCTGAAGATGTGAGCCAGCGAGTCTATCTCTATGAGGGACTCCTAGGCAAAGAGCGTTCTACTTTATGGGACCAAATGCAATTCTGGGAA GATGCCTTCTTAGATGCTGTGATGTTGGAGAGAGAAGGGATGGGTATGGACCAGGGTCCCCAGGAAATGATCGACAGGTACCTGTCCCTTGGAGAACATG ACCGGAAGCGCCTGGAAGATGATGAAGATCGCTTGTTGGCCACACTTCTGCACAACCTCATCTCCTACATGCTGCTGATGAAGGTAAATAAGAATGACAT CCGCAAGAAGGTGAGGCGCCTAATGGGAAAGTCGCACATTGGGCTTGTGTACAGCCAGCAAATCAATGAGGTGCTTGATCAGCTGGCGAACCTGAATGGA CGCGATCTCTCTATCTGGTCCAGTGGCAGCCGGCACATGAAGAAGCAGACATTTGTGGTACATGCAGGGACAGATACAAACGGAGATATCTTTTTCATGG AGGTGTGCGATGACTGTGTGGTGTTGCGTAGTAACATCGGAACAGTGTATGAGCGCTGGTGGTACGAGAAGCTCATCAACATGACCTACTGTCCCAAGAC GAAGGTGTTGTGCTTGTGGCGTAGAAATGGCTCTGAGACCCAGCTCAACAAGTTCTATACTAAAAAGTGTCGGGAGCTGTACTACTGTGTGAAGGACAGC ATGGAGCGCGCTGCCGCCCGACAGCAAAGCATCAAACCCGGACCTGAATTGGGTGGCGAGTTCCCTGTGCAGGACCTGAAGACTGGTGAGGGTGGCCTGC TGCAGGTGACCCTGGAAGGGATCAACCTCAAATTCATGCACAATCAGGTTTTCATAGAGCTGAATCACATTAAAAAGTGCAATACAGTTCGAGGCGTCTT TGTCCTGGAGGAATTTGTTCCTGAAATTAAAGAAGTGGTGAGCCACAAGTACAAGACACCAATGGCCCACGAAATCTGCTACTCCGTATTATGTCTCTTC TCGTACGTGGCTGCAGTTCATAGCAGTGAGGAAGATCTCAGAACCCCGCCCCGGCCTGTCTCTAGCTGATGGAGAGGGGCTACGCAGCTGCCCCAGCCCA GGGCACGCCCCTGGCCCCTTGCTGTTCCCAAGTGCACGATGCTGCTGTGACTGAGGAGTGGATGATGCTCGTGTGTCCTCTGCAAGCCCCCTGCTGTGGC TTGGTTGGTTACCGGTTATGTGTCCCTCTGAGTGTGTCTTGAGCGTGTCCACCTTCTCCCTCTCCACTCCCAGAAGACCAAACTGCCTTCCCCTCAGGGC TCAAGAATGTGTACAGTCTGTGGGGCCGGTGTGAACCCACTATTTTGTGTCCTTGAGACATTTGTGTTGTGGTTCCTTGTCCTTGTCCCTGGCGTTATAA CTGTCCACTGCAAGAGTCTGGCTCTCCCTTCTCTGTGACCCGGCATGACTGGGCGCCTGGAGCAGTTTCACTCTGTGAGGAGTGAGGGAACCCTGGGGCT CACCCTCTCAGAGGAAGGGCACAGAGAGGAAGGGAAGAATTGGGGGGCAGCCGGAGTGAGTGGCAGCCTCCCTGCTTCCTTCTGCATTCCCAAGCCGGCA GCTACTGCCCAGGGCCCGCAGTGTTGGCTGCTGCCTGCCACAGCCTCTGTGACTGCAGTGGAGCGGCGAATTCCCTGTGGCCTGCCATGCCTTCGGCATC AGAGGATGGAGTGGTCGAGGCTAGTGGAGTCCCAGGGACCGCTGGCTGCTCTGCCTGAGCATCAGGGAGGGGGCAGGAAAGACCAAGCTGGGTTTGCACA TCTGTCTGCAGGCTGTCTCTCCAGGCACGGGGTGTCAGGAGGGAGAGACAGCCTGGGTATGGGCAAGAAATGACTGTAAATATTTCAGCCCCACATTATT TATAGAAAATGTACAGTTGTGTGAATGTGAAATAAATGTCCTCAACTCCC

refseq_MADD.F8 (forward primer)
refseq_MADD.R8 (reverse primer)
Single exon skipping

Frame 0

SADAELAPARSGAALGSD*RPAAGGRRAGPGQS PRAWETSIFRIPPGNADSLLGALMLL*DKLMNWN HGAKEEVLSSVT*LSSDRRGQAPEQ**RGPDS* IATAIPLGGSH*VSPAPRCSVLLPARGLPERAA AAHEPSG*YLFCLHPH*QGHWSHAIWHLC*LLPL LPKANL*GEGGRWGRVPWEGRNPCHLCLRRGWH *ELREWLIPAASQC*LYP*CEPVSSGQTPGQGG EPLPQQYSHVPVRAQPLPFLLHLPRVFVYSQAPG GLL**APSGQETGHPSRRTKGHHVADLYWIAAG RGEVKCPSA*PSRD*GLDLSIAALPSTRLWAEA SRHRGPTPRAPASSDLCSSRPISIHPSGFPTAPS LGTSRCGRLSPGANLHSVRAQGGATVPRLQCTL HVCDGIRGNDLPTGVYVSCHPAATHLHGISRAA AVGSNPVHHWGSCQLLPLQTGLQNA**CMASGSG QQ*GDCPHQCRSAAYPARTRITRAEKAFKAGLG QHESQHPAHPQSGEIS*GPGDPPSLGKAF**PA VHTVH*IQPTHLWQ*CGFCGCCNQGCHGTVLQFR QRAAGISDAHAYPAPLSSACGSFSSWLLSSLTS PADSFCREIGQDSGCGVLWGMDP*PHQLCLSAN SQQYV*SSPDW*QAKVVCSSAAAYPLSRL*QQFP AG*GPECTTRAGL*LRTY***WQ**YGL*RFKL FLLLPW*LCQ*NDEM*H*W*YSQCGPSDTCSTG GCQRGGD*RAAESEGSRRAWPRQ*ELSGKPPTAL QL*HHSQQQPQHCHPRSQL*TC*LYGDG**GSS RRLQAPPFRASQHWQIERGQTSGRNWRGVSAPA NL*QSILRAPIWLSP*GR*G*AGGKLHSPIQPTC QWQSGSKAAAAQQLETGK*LRCRVRLSGKLSQL HRLQHQHRGLRGHHVFCQQPLSEPQYQLQSFKP HTAHQRCPREGHALPQSERKQEGVSGSEVICH*T QPNSEKRTSITPGSIQQF**EPAVPEGGGAQRA GRPGSWLAQHEKGAPAAGERAAASLCPEQAEPH GAVRGRCPAGHHPGCGDQSEGVQGNVRPPQVYSP QLGAVLCPRGSGWHGQHLWAFGDCPDPLL**RT RQAEEKSNRKCKYPSWQGSWPSWAGGPKGYGTT ESSTTGTSGTKCHRKGS*GTGHQKFKGRKFYSIY WA*SNQTCL*PW*DRGEKVPDQRRQWCEPDV*F PED*SRLCHRRESSCYDPQLKSGF*S*HRGE** LWRDPWS*Q*LEQQCR*WTRWRGQCSPGKLSGHF V***N*DQLCHKHHLW*SPQLEAKHKGEAGRQP HSYF*RCEPASLSL*GTPRQRAFYFMGPNAILG RCLLRCCDVGERRDGYGPGSPGNDRQVPVPWRT* PEAPGR**RSLVGHTSAQPHLLHAADEGK*E*H PQEGEAPNGKVAHWACVQPANQ*GA*SAGEPEW TRSLYLVQWQPAHEEADICGTCRDRYKRRYLFHG GVR*LCGVA**HRNSV*ALVVREAHQHDLLSQD EGVVLVA*KWL*DPAQQVLY*KVSGAVLLCEGQ HGARCRPTAKHQTRT*IGWRVPCAGPEDW*GWPA AGDPGRDQPQIHAQSGFHRAESH*KVQYSSRRL CPGGICS*N*RSGEPQVQDTNGPRNLLLRIMSL LVRGCSS*Q*GRSQNPAPACL*LMERGYAAAPAQ GTPLAPCCSQVHDAAVTEEWMMLVCPLQAPCCG LVGYRLCVPLSVS*ACPPSPSPLPEDQTAFPSG LKNVYSLWGRCEPTILCP*DICVVVPCPCPWRYN CPLQESGSPFSVTRHDWAPGAVSLCEE*GNPGA HPLRGRAQRGREELGGSRSEWQPPCFLLHSQAG SYCPGPAVLAAACHSLCDCSGAANSLWPAMPSAS EDGVVEASGVPGTAGCSA*ASGRGQERPSWVCT SVCRLSLQARGVRRERQPGYGQEMTVNISAPHY L*KMYSCVNVK*MSSTP

Frame 1

VQTRNWRPLGAAPRWGATDAPLPGDVGLGLASP PELGRLRFSEFLLGMLTPCLVP*CFSEIN**IG TMVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPE LLRRYPLEDHTEFPLPPDVVFFCQPEGCLSVRQ RRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYR SFQKRISKEKGEGGAGSRGKEGTHATCASEEGGT ESSESGSSLQPLSADSTPDVNQSPRGKRRAKAG SRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRL VDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLV EEKSSALLHDLREIEAWIYRLLRSPVPVSGQKR VDIEVLPQELQPALTFALPDPSRFTLVDFPLHL PLELLGVDACLQVLTCILLEHKVVLQSRDYNALS MSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQL LLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDL DSNRVIAPTNAEVLPILPEPESLELKKHLKQALA SMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQ STPSTEFNPLIYGNDVDSVDVATRVAMVRFFNS ANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRP RQTPFAEKLARTQAVEYFGEWILNPTNYAFQRI HNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNS QLAEALSVPPERDSDSEPTDDSGSDSMDYDDSSS SYSSLGDFVSEMMKCDINGDTPNVDPLTHAALG DASEVEIDELQNQKEAEEPGPDSENSQENPPLR SSSSTTASSSPSTVIHGANSEPADSTEMDDKAAV GVSKPLPSVPPSIGKSNVDRRQAEIGEGSVRRR IYDNPYFEPQYGFPPEEDEDEQGESYTPRFSQH VSGNRAQKLLRPNSLRLASDSDAESDSRASSPNS TVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNL TLPTKGAREKATPFPSLKGNRRALVDQKSSVIK HSPTVKREPPSPQGRSSNSSENQQFLKEVVHSVL DGQGVGWLNMKKVRRLLESEQLRVFVLSKLNRM VQSEDDARQDIIPDVEISRKVYKGMLDLLKCTV LSLEQSYAHAGLGGMASIFGLLEIAQTHYYSKEP DKRKRSPTESVNTPVGKDPGLAGRGDPKAMAQL RVPQLGPRAPSATGKGPKELDTRSLKEENFIAS IGPEVIKPVFDLGETEEKKSQISADSGVSLTSSS QRTDQDSVIGVSPAVMIRSSSQDSEVSTVVSNS SGETLGADSDLSSNAGDGPGGEGSVHLASSRGT LSDSEIETNSATSTIFGKAHSLKPSIKEKLAGSP IRTSEDVSQRVYLYEGLLGKERSTLWDQMQFWE DAFLDAVMLEREGMGMDQGPQEMIDRYLSLGEH DRKRLEDDEDRLLATLLHNLISYMLLMKVNKNDI RKKVRRLMGKSHIGLVYSQQINEVLDQLANLNG RDLSIWSSGSRHMKKQTFVVHAGTDTNGDIFFM EVCDDCVVLRSNIGTVYERWWYEKLINMTYCPKT KVLCLWRRNGSETQLNKFYTKKCRELYYCVKDS MERAAARQQSIKPGPELGGEFPVQDLKTGEGGL LQVTLEGINLKFMHNQVFIELNHIKKCNTVRGVF VLEEFVPEIKEVVSHKYKTPMAHEICYSVLCLF SYVAAVHSSEEDLRTPPRPVSS*WRGATQLPQP RARPWPLAVPKCTMLL*LRSG*CSCVLCKPPAVA WLVTGYVSL*VCLERVHLLPLHSQKTKLPSPQG SRMCTVCGAGVNPLFCVLETFVLWFLVLVPGVI TVHCKSLALPSL*PGMTGRLEQFHSVRSEGTLGL TLSEEGHREEGKNWGAAGVSGSLPASFCIPKPA ATAQGPQCWLLPATASVTAVERRIPCGLPCLRH QRMEWSRLVESQGPLAALPEHQGGGRKDQAGFAH LSAGCLSRHGVSGGRDSLGMGKK*L*IFQPHII YRKCTVV*M*NKCPQL

Frame 2

CRRGTGARSERRRAGERLTPRCRGTSGWAWPVP QSLGDFDFQNSSWEC*LLAWCPDASLR*TDELE PWCKRRSSVLGYLTI**S*GPGTRAVIAWPRLL NCYGDTPWRITLSFPCPQM*CSSASPRAA*ACGS GA*AFGMIPLLSSPSLTRTLESRDMASVLTSTA PSKSESLRRRGKVGQGPVGRKEPMPPVPQKRVA LRAQRVAHPCSLSVLTLPLM*TSLLGANAGPRRG AAPATVLSRPCACSATTLSSPPSESVCILSSAW WTAVVSAFWARNWASLEAYKGTPCGGSLLDRCW *RRSQVPFCMTFERLRPGSIDCCAPQYPSLGRSE *TSRSYPKSSSQL*PLLFQTHLDSP*WISHCTF PWNF*VWTPVSRC*PAFC*STRWCYSPETTMHS PCL*WHSWQ*STHWSICFLSSRCYPPAWHQQSSC CWLQPRTSLGFLPASSSTNWTSKCLMMYG*WIW TAIG*LPPPMQKCCLSCQNQNH*S*KSI*SRPW PA*VSTPSPSSIWRNFMRARRSPFSWEGLLMTCS PHRPLNSTHSSMAMMWILWMLQPGLPWYGSSIP PTCCRDFRCTRVPCASFLGLW*LFKLAPF*PHV PGRLLLPRNWPGLRLWSTLGNGSLTPPTMPFSEF TTICLIQP*LVTSQSGMLISCSLSTIASMTAIP SWLRP*VYHQSGTLTPNLLMIVAVIVWIMTIQA LLTPPLVTLSVK**NVTLMVILPMWTL*HMQHWG MPARWRLTSCRIRRKQKSLAQTVRTLRKTPHCA PALAPQPAAAPALSSTEPTLNLLTLRRWMIRQQ *ASPSPSLPCLPALANRTWTDVRQKLERGQCAGE SMTIHTSSPNMAFPLRKMRMSRGKVTLPDSANM SVAIGLKSCCGPTA*DWQVTQMQSQTLGQALPT PPSPTPAPRASGASCLLPAASIGTTVPASVFQTS HCPPKVPERRPRPSPV*KETGGR*WIRSHLSLN TAQQ*KENLHHPRVDPAILVRTSSS*RRWCTAC WTARELAGST*KRCAGCWRASSCESLS*AS*TAW CSQRTMPGRTSSRMWRSVGRCTREC*TSSSVQS SAWSSPMPTRVWVAWPASLGFWRLPRPTTIVKN QTSGREVQQKV*IPQLARILA*LGGGTQRLWHN* EFHNWDLGHQVPQERVLRNWTPEV*RKKIL*HL LGLK*SNLSLTLVRQRRKSPRSAQTVV*A*RLV PRGLIKTLSSA*VQLL*SAAQVRILKLAPW*VIA LERPLELTVT*AAMQVMDQVARAVFTWQALGAL CLIVKLRPTLPQAPSLVKPTA*SQA*RRSWQAA PFVLLKM*ASESISMRDS*AKSVLLYGTKCNSGK MPS*ML*CWREKGWVWTRVPRK*STGTCPLENM TGSAWKMMKIACWPHFCTTSSPTCC**R*IRMT SARR*GA*WESRTLGLCTASKSMRCLISWRT*MD AISLSGPVAAGT*RSRHLWYMQGQIQTEISFSW RCAMTVWCCVVTSEQCMSAGGTRSSST*PTVPR RRCCACGVEMALRPSSTSSILKSVGSCTTV*RTA WSALPPDSKASNPDLNWVASSLCRT*RLVRVAC CR*PWKGSTSNSCTIRFS*S*ITLKSAIQFEAS LSWRNLFLKLKKW*ATSTRHQWPTKSATPYYVSS RTWLQFIAVRKISEPRPGLSLADGEGLRSCPSP GHAPGPLLFPSARCCCD*GVDDARVSSASPLLW LGWLPVMCPSECVLSVSTFSLSTPRRPNCLPLRA QECVQSVGPV*THYFVSLRHLCCGSLSLSLAL* LSTARVWLSLLCDPA*LGAWSSFTL*GVREPWG SPSQRKGTERKGRIGGQPE*VAASLLPSAFPSRQ LLPRARSVGCCLPQPL*LQWSGEFPVACHAFGI RGWSGRG*WSPRDRWLLCLSIREGAGKTKLGLH ICLQAVSPGTGCQEGETAWVWARNDCKYFSPTLF IENVQLCECEINVLNS

Sequence NM_130472

ACCGCGGCCGCGCGCCCCCCTCTGCCCCCGCTGCCCAGGATTGGTAGGCTCCACCGCTCGGCAGCCGGCTTCCCTGCTCGGACGCCGAGCACCGCCAAAG CGCACTTCGATTTTCAGAATTCCTCCTGGGAATGCTGACTCCTTGCTTGGTGCCCTGATGCTTCTCTGAGATAAACTGATGAATTGGAACCATGGTGCAA AAGAAGAAGTTCTGTCCTCGGTTACTTGACTATCTAGTGATCGTAGGGGCCAGGCACCCGAGCAGTGATAGCGTGGCCCAGACTCCTGAATTGCTACGGC GATACCCCTTGGAGGATCACACTGAGTTTCCCCTGCCCCCAGATGTAGTGTTCTTCTGCCAGCCCGAGGGCTGCCTGAGCGTGCGGCAGCGGCGCATGAG CCTTCGGGATGATACCTCTTTTGTCTTCACCCTCACTGACAAGGACACTGGAGTCACGCGATATGGCATCTGTGTTAACTTCTACCGCTCCTTCCAAAAG CGAATCTCTAAGGAGAAGGGGGAAGGTGGGGCAGGGTCCCGTGGGAAGGAAGGAACCCATGCCACCTGTGCCTCAGAAGAGGGTGGCACTGAGAGCTCAG AGAGTGGCTCATCCCTGCAGCCTCTCAGTGCTGACTCTACCCCTGATGTGAACCAGTCTCCTCGGGGCAAACGCCGGGCCAAGGCGGGGAGCCGCTCCCG CAACAGTACTCTCACGTCCCTGTGCGTGCTCAGCCACTACCCTTTCTTCTCCACCTTCCGAGAGTGTTTGTATACTCTCAAGCGCCTGGTGGACTGCTGT AGTGAGCGCCTTCTGGGCAAGAAACTGGGCATCCCTCGAGGCGTACAAAGGGACACCATGTGGCGGATCTTTACTGGATCGCTGCTGGTAGAGGAGAAGT CAAGTGCCCTTCTGCATGACCTTCGAGAGATTGAGGCCTGGATCTATCGATTGCTGCGCTCCCCAGTACCCGTCTCTGGGCAGAAGCGAGTAGACATCGA GGTCCTACCCCAAGAGCTCCAGCCAGCTCTGACCTTTGCTCTTCCAGACCCATCTCGATTCACCCTAGTGGATTTCCCACTGCACCTTCCCTTGGAACTT CTAGGTGTGGACGCCTGTCTCCAGGTGCTAACCTGCATTCTGTTAGAGCACAAGGTGGTGCTACAGTCCCGAGACTACAATGCACTCTCCATGTCTGTGA TGGCATTCGTGGCAATGATCTACCCACTGGAGTATATGTTTCCTGTCATCCCGCTGCTACCCACCTGCATGGCATCAGCAGAGCAGCTGCTGTTGGCTCC AACCCCGTACATCATTGGGGTTCCTGCCAGCTTCTTCCTCTACAAACTGGACTTCAAAATGCCTGATGATGTATGGCTAGTGGATCTGGACAGCAATAGG GTGATTGCCCCCACCAATGCAGAAGTGCTGCCTATCCTGCCAGAACCAGAATCACTAGAGCTGAAAAAGCATTTAAAGCAGGCCTTGGCCAGCATGAGTC TCAACACCCAGCCCATCCTCAATCTGGAGAAATTTCATGAGGGCCAGGAGATCCCCCTTCTCTTGGGAAGGCCTTCTAATGACCTGCAGTCCACACCGTC CACTGAATTCAACCCACTCATCTATGGCAATGATGTGGATTCTGTGGATGTTGCAACCAGGGTTGCCATGGTACGGTTCTTCAATTCCGCCAACGTGCTG CAGGGATTTCAGATGCACACGCGTACCCTGCGCCTCTTTCCTCGGCCTGTGGTAGCTTTTCAAGCTGGCTCCTTTCTAGCCTCACGTCCCCGGCAGACTC CTTTTGCCGAGAAATTGGCCAGGACTCAGGCTGTGGAGTACTTTGGGGAATGGATCCTTAACCCCACCAACTATGCCTTTCAGCGAATTCACAACAATAT GTTTGATCCAGCCCTGATTGGTGACAAGCCAAAGTGGTATGCTCATCAGCTGCAGCCTATCCACTATCGCGTCTATGACAGCAATTCCCAGCTGGCTGAG GCCCTGAGTGTACCACCAGAGCGGGACTCTGACTCCGAACCTACTGATGATAGTGGCAGTGATAGTATGGATTATGACGATTCAAGCTCTTCTTACTCCT CCCTTGGTGACTTTGTCAGTGAAATGATGAAATGTGACATTAATGGTGATACTCCCAATGTGGACCCTCTGACACATGCAGCACTGGGGGATGCCAGCGA GGTGGAGATTGACGAGCTGCAGAATCAGAAGGAAGCAGAAGAGCCTGGCCCAGACAGTGAGAACTCTCAGGAAAACCCCCCACTGCGCTCCAGCTCTAGC ACCACAGCCAGCAGCAGCCCCAGCACTGTCATCCACGGAGCCAACTCTGAACCTGCTGACTCTACGGAGATGGATGATAAGGCAGCAGTAGGCGTCTCCA AGCCCCTCCCTTCCGTGCCTCCCAGCATTGGCAAATCGAACGTGGACAGACGTCAGGCAGAAATTGGAGAGGGGGCTCAAAAGCTGCTGCGGCCCAACAG CTTGAGACTGGCAAGTGACTCAGATGCAGAGTCAGACTCTCGGGCAAGCTCTCCCAACTCCACCGTCTCCAACACCAGCACCGAGGGCTTCGGGGGCATC ATGTCTTTTGCCAGCAGCCTCTATCGGAACCACAGTACCAGCTTCAGTCTTTCAAACCTCACACTGCCCACCAAAGGTGCCCGAGAGAAGGCCACGCCCT TCCCCAGTCTGAAAGGAAACAGGAGGGCGTTAGTGGATCAGAAGTCATCTGTCATTAAACACAGCCCAACAGTGAAAAGAGAACCTCCATCACCCCAGGG TCGATCCAGCAATTCTAGTGAGAACCAGCAGTTCCTGAAGGAGGTGGTGCACAGCGTGCTGGACGGCCAGGGAGTTGGCTGGCTCAACATGAAAAAGGTG CGCCGGCTGCTGGAGAGCGAGCAGCTGCGAGTCTTTGTCCTGAGCAAGCTGAACCGCATGGTGCAGTCAGAGGACGATGCCCGGCAGGACATCATCCCGG ATGTGGAGATCAGTCGGAAGGTGTACAAGGGAATGTTAGACCTCCTCAAGTGTACAGTCCTCAGCTTGGAGCAGTCCTATGCCCACGCGGGTCTGGGTGG CATGGCCAGCATCTTTGGGCTTTTGGAGATTGCCCAGACCCACTACTATAGTAAAGAACCAGACAAGCGGAAGAGAAGTCCAACAGAAAGTGTAAATACC CCAGTTGGCAAGGATCCTGGCCTAGCTGGGCGGGGGGACCCAAAGGCTATGGCACAACTGAGAGTTCCACAACTGGGACCTCGGGCACCAAGTGCCACAG GAAAGGGTCCTAAGGAACTGGACACCAGAAGTTTAAAGGAAGAAAATTTTATAGCATCTATTGGGCCTGAAGTAATCAAACCTGTCTTTGACCTTGGTGA GACAGAGGAGAAAAAGTCCCAGATCAGCGCAGACAGTGGTGTGAGCCTGACGTCTAGTTCCCAGAGGACTGATCAAGACTCTGTCATCGGCGTGAGTCCA GCTGTTATGATCCGCAGCTCAAGTCAGGATTCTGAAGTTAGCACCGTGGTGAGTAATAGCTCTGGAGAGACCCTTGGAGCTGACAGTGACTTGAGCAGCA ATGCAGGTGATGGACCAGGTGGCGAGGGCAGTGTTCACCTGGCAAGCTCTCGGGGCACTTTGTCTGATAGTGAAATTGAGACCAACTCTGCCACAAGCAC CATCTTTGGTAAAGCCCACAGCTTGAAGCCAAGCATAAAGGAGAAGCTGGCAGGCAGCCCCATTCGTACTTCTGAAGATGTGAGCCAGCGAGTCTATCTC TATGAGGGACTCCTAGGCAAAGAGCGTTCTACTTTATGGGACCAAATGCAATTCTGGGAAGATGCCTTCTTAGATGCTGTGATGTTGGAGAGAGAAGGGA TGGGTATGGACCAGGGTCCCCAGGAAATGATCGACAGGTACCTGTCCCTTGGAGAACATGACCGGAAGCGCCTGGAAGATGATGAAGATCGCTTGTTGGC CACACTTCTGCACAACCTCATCTCCTACATGCTGCTGATGAAGGTAAATAAGAATGACATCCGCAAGAAGGTGAGGCGCCTAATGGGAAAGTCGCACATT GGGCTTGTGTACAGCCAGCAAATCAATGAGGTGCTTGATCAGCTGGCGAACCTGAATGGACGCGATCTCTCTATCTGGTCCAGTGGCAGCCGGCACATGA AGAAGCAGACATTTGTGGTACATGCAGGGACAGATACAAACGGAGATATCTTTTTCATGGAGGTGTGCGATGACTGTGTGGTGTTGCGTAGTAACATCGG AACAGTGTATGAGCGCTGGTGGTACGAGAAGCTCATCAACATGACCTACTGTCCCAAGACGAAGGTGTTGTGCTTGTGGCGTAGAAATGGCTCTGAGACC CAGCTCAACAAGTTCTATACTAAAAAGTGTCGGGAGCTGTACTACTGTGTGAAGGACAGCATGGAGCGCGCTGCCGCCCGACAGCAAAGCATCAAACCCG GACCTGAATTGGGTGGCGAGTTCCCTGTGCAGGACCTGAAGACTGGTGAGGGTGGCCTGCTGCAGGTGACCCTGGAAGGGATCAACCTCAAATTCATGCA CAATCAGGTTTTCATAGAGCTGAATCACATTAAAAAGTGCAATACAGTTCGAGGCGTCTTTGTCCTGGAGGAATTTGTTCCTGAAATTAAAGAAGTGGTG AGCCACAAGTACAAGACACCAATGGCCCACGAAATCTGCTACTCCGTATTATGTCTCTTCTCGTACGTGGCTGCAGTTCATAGCAGTGAGGAAGATCTCA GAACCCCGCCCCGGCCTGTCTCTAGCTGATGGAGAGGGGCTACGCAGCTGCCCCAGCCCAGGGCACGCCCCTGGCCCCTTGCTGTTCCCAAGTGCACGAT GCTGCTGTGACTGAGGAGTGGATGATGCTCGTGTGTCCTCTGCAAGCCCCCTGCTGTGGCTTGGTTGGTTACCGGTTATGTGTCCCTCTGAGTGTGTCTT GAGCGTGTCCACCTTCTCCCTCTCCACTCCCAGAAGACCAAACTGCCTTCCCCTCAGGGCTCAAGAATGTGTACAGTCTGTGGGGCCGGTGTGAACCCAC TATTTTGTGTCCTTGAGACATTTGTGTTGTGGTTCCTTGTCCTTGTCCCTGGCGTTATAACTGTCCACTGCAAGAGTCTGGCTCTCCCTTCTCTGTGACC CGGCATGACTGGGCGCCTGGAGCAGTTTCACTCTGTGAGGAGTGAGGGAACCCTGGGGCTCACCCTCTCAGAGGAAGGGCACAGAGAGGAAGGGAAGAAT TGGGGGGCAGCCGGAGTGAGTGGCAGCCTCCCTGCTTCCTTCTGCATTCCCAAGCCGGCAGCTACTGCCCAGGGCCCGCAGTGTTGGCTGCTGCCTGCCA CAGCCTCTGTGACTGCAGTGGAGCGGCGAATTCCCTGTGGCCTGCCATGCCTTCGGCATCAGAGGATGGAGTGGTCGAGGCTAGTGGAGTCCCAGGGACC GCTGGCTGCTCTGCCTGAGCATCAGGGAGGGGGCAGGAAAGACCAAGCTGGGTTTGCACATCTGTCTGCAGGCTGTCTCTCCAGGCACGGGGTGTCAGGA GGGAGAGACAGCCTGGGTATGGGCAAGAAATGACTGTAAATATTTCAGCCCCACATTATTTATAGAAAATGTACAGTTGTGTGAATGTGAAATAAATGTC CTCAACTCCC

refseq_MADD.F8 (forward primer)
refseq_MADD.R8 (reverse primer)
Single exon skipping

Frame 0

TAAARPPLPPLPRIGRLHRSAAGFPARTPSTAK AHFDFQNSSWEC*LLAWCPDASLR*TDELEPWCK RRSSVLGYLTI**S*GPGTRAVIAWPRLLNCYG DTPWRITLSFPCPQM*CSSASPRAA*ACGSGA* AFGMIPLLSSPSLTRTLESRDMASVLTSTAPSKS ESLRRRGKVGQGPVGRKEPMPPVPQKRVALRAQ RVAHPCSLSVLTLPLM*TSLLGANAGPRRGAAP ATVLSRPCACSATTLSSPPSESVCILSSAWWTAV VSAFWARNWASLEAYKGTPCGGSLLDRCW*RRS QVPFCMTFERLRPGSIDCCAPQYPSLGRSE*TS RSYPKSSSQL*PLLFQTHLDSP*WISHCTFPWNF *VWTPVSRC*PAFC*STRWCYSPETTMHSPCL* WHSWQ*STHWSICFLSSRCYPPAWHQQSSCCWL QPRTSLGFLPASSSTNWTSKCLMMYG*WIWTAIG *LPPPMQKCCLSCQNQNH*S*KSI*SRPWPA*V STPSPSSIWRNFMRARRSPFSWEGLLMTCSPHR PLNSTHSSMAMMWILWMLQPGLPWYGSSIPPTCC RDFRCTRVPCASFLGLW*LFKLAPF*PHVPGRL LLPRNWPGLRLWSTLGNGSLTPPTMPFSEFTTI CLIQP*LVTSQSGMLISCSLSTIASMTAIPSWLR P*VYHQSGTLTPNLLMIVAVIVWIMTIQALLTP PLVTLSVK**NVTLMVILPMWTL*HMQHWGMPA RWRLTSCRIRRKQKSLAQTVRTLRKTPHCAPALA PQPAAAPALSSTEPTLNLLTLRRWMIRQQ*ASP SPSLPCLPALANRTWTDVRQKLERGLKSCCGPT A*DWQVTQMQSQTLGQALPTPPSPTPAPRASGAS CLLPAASIGTTVPASVFQTSHCPPKVPERRPRP SPV*KETGGR*WIRSHLSLNTAQQ*KENLHHPR VDPAILVRTSSS*RRWCTACWTARELAGST*KRC AGCWRASSCESLS*AS*TAWCSQRTMPGRTSSR MWRSVGRCTREC*TSSSVQSSAWSSPMPTRVWV AWPASLGFWRLPRPTTIVKNQTSGREVQQKV*IP QLARILA*LGGGTQRLWHN*EFHNWDLGHQVPQ ERVLRNWTPEV*RKKIL*HLLGLK*SNLSLTLV RQRRKSPRSAQTVV*A*RLVPRGLIKTLSSA*VQ LL*SAAQVRILKLAPW*VIALERPLELTVT*AA MQVMDQVARAVFTWQALGALCLIVKLRPTLPQA PSLVKPTA*SQA*RRSWQAAPFVLLKM*ASESIS MRDS*AKSVLLYGTKCNSGKMPS*ML*CWREKG WVWTRVPRK*STGTCPLENMTGSAWKMMKIACW PHFCTTSSPTCC**R*IRMTSARR*GA*WESRTL GLCTASKSMRCLISWRT*MDAISLSGPVAAGT* RSRHLWYMQGQIQTEISFSWRCAMTVWCCVVTS EQCMSAGGTRSSST*PTVPRRRCCACGVEMALRP SSTSSILKSVGSCTTV*RTAWSALPPDSKASNP DLNWVASSLCRT*RLVRVACCR*PWKGSTSNSC TIRFS*S*ITLKSAIQFEASLSWRNLFLKLKKW* ATSTRHQWPTKSATPYYVSSRTWLQFIAVRKIS EPRPGLSLADGEGLRSCPSPGHAPGPLLFPSAR CCCD*GVDDARVSSASPLLWLGWLPVMCPSECVL SVSTFSLSTPRRPNCLPLRAQECVQSVGPV*TH YFVSLRHLCCGSLSLSLAL*LSTARVWLSLLCD PA*LGAWSSFTL*GVREPWGSPSQRKGTERKGRI GGQPE*VAASLLPSAFPSRQLLPRARSVGCCLP QPL*LQWSGEFPVACHAFGIRGWSGRG*WSPRD RWLLCLSIREGAGKTKLGLHICLQAVSPGTGCQE GETAWVWARNDCKYFSPTLFIENVQLCECEINV LNS

Frame 1

PRPRAPLCPRCPGLVGSTARQPASLLGRRAPPK RTSIFRIPPGNADSLLGALMLL*DKLMNWNHGA KEEVLSSVT*LSSDRRGQAPEQ**RGPDS*IATA IPLGGSH*VSPAPRCSVLLPARGLPERAAAAHE PSG*YLFCLHPH*QGHWSHAIWHLC*LLPLLPK ANL*GEGGRWGRVPWEGRNPCHLCLRRGWH*ELR EWLIPAASQC*LYP*CEPVSSGQTPGQGGEPLP QQYSHVPVRAQPLPFLLHLPRVFVYSQAPGGLL **APSGQETGHPSRRTKGHHVADLYWIAAGRGEV KCPSA*PSRD*GLDLSIAALPSTRLWAEASRHR GPTPRAPASSDLCSSRPISIHPSGFPTAPSLGT SRCGRLSPGANLHSVRAQGGATVPRLQCTLHVCD GIRGNDLPTGVYVSCHPAATHLHGISRAAAVGS NPVHHWGSCQLLPLQTGLQNA**CMASGSGQQ* GDCPHQCRSAAYPARTRITRAEKAFKAGLGQHES QHPAHPQSGEIS*GPGDPPSLGKAF**PAVHTV H*IQPTHLWQ*CGFCGCCNQGCHGTVLQFRQRA AGISDAHAYPAPLSSACGSFSSWLLSSLTSPADS FCREIGQDSGCGVLWGMDP*PHQLCLSANSQQY V*SSPDW*QAKVVCSSAAAYPLSRL*QQFPAG* GPECTTRAGL*LRTY***WQ**YGL*RFKLFLLL PW*LCQ*NDEM*H*W*YSQCGPSDTCSTGGCQR GGD*RAAESEGSRRAWPRQ*ELSGKPPTALQL* HHSQQQPQHCHPRSQL*TC*LYGDG**GSSRRLQ APPFRASQHWQIERGQTSGRNWRGGSKAAAAQQ LETGK*LRCRVRLSGKLSQLHRLQHQHRGLRGH HVFCQQPLSEPQYQLQSFKPHTAHQRCPREGHAL PQSERKQEGVSGSEVICH*TQPNSEKRTSITPG SIQQF**EPAVPEGGGAQRAGRPGSWLAQHEKG APAAGERAAASLCPEQAEPHGAVRGRCPAGHHPG CGDQSEGVQGNVRPPQVYSPQLGAVLCPRGSGW HGQHLWAFGDCPDPLL**RTRQAEEKSNRKCKY PSWQGSWPSWAGGPKGYGTTESSTTGTSGTKCHR KGS*GTGHQKFKGRKFYSIYWA*SNQTCL*PW* DRGEKVPDQRRQWCEPDV*FPED*SRLCHRRES SCYDPQLKSGF*S*HRGE**LWRDPWS*Q*LEQQ CR*WTRWRGQCSPGKLSGHFV***N*DQLCHKH HLW*SPQLEAKHKGEAGRQPHSYF*RCEPASLS L*GTPRQRAFYFMGPNAILGRCLLRCCDVGERRD GYGPGSPGNDRQVPVPWRT*PEAPGR**RSLVG HTSAQPHLLHAADEGK*E*HPQEGEAPNGKVAH WACVQPANQ*GA*SAGEPEWTRSLYLVQWQPAHE EADICGTCRDRYKRRYLFHGGVR*LCGVA**HR NSV*ALVVREAHQHDLLSQDEGVVLVA*KWL*D PAQQVLY*KVSGAVLLCEGQHGARCRPTAKHQTR T*IGWRVPCAGPEDW*GWPAAGDPGRDQPQIHA QSGFHRAESH*KVQYSSRRLCPGGICS*N*RSG EPQVQDTNGPRNLLLRIMSLLVRGCSS*Q*GRSQ NPAPACL*LMERGYAAAPAQGTPLAPCCSQVHD AAVTEEWMMLVCPLQAPCCGLVGYRLCVPLSVS *ACPPSPSPLPEDQTAFPSGLKNVYSLWGRCEPT ILCP*DICVVVPCPCPWRYNCPLQESGSPFSVT RHDWAPGAVSLCEE*GNPGAHPLRGRAQRGREE LGGSRSEWQPPCFLLHSQAGSYCPGPAVLAAACH SLCDCSGAANSLWPAMPSASEDGVVEASGVPGT AGCSA*ASGRGQERPSWVCTSVCRLSLQARGVR RERQPGYGQEMTVNISAPHYL*KMYSCVNVK*MS STP

Frame 2

RGRAPPSAPAAQDW*APPLGSRLPCSDAEHRQS ALRFSEFLLGMLTPCLVP*CFSEIN**IGTMVQ KKKFCPRLLDYLVIVGARHPSSDSVAQTPELLR RYPLEDHTEFPLPPDVVFFCQPEGCLSVRQRRMS LRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQK RISKEKGEGGAGSRGKEGTHATCASEEGGTESS ESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSR NSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCC SERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEK SSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIE VLPQELQPALTFALPDPSRFTLVDFPLHLPLEL LGVDACLQVLTCILLEHKVVLQSRDYNALSMSV MAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAP TPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNR VIAPTNAEVLPILPEPESLELKKHLKQALASMS LNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPS TEFNPLIYGNDVDSVDVATRVAMVRFFNSANVL QGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQT PFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNM FDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAE ALSVPPERDSDSEPTDDSGSDSMDYDDSSSSYS SLGDFVSEMMKCDINGDTPNVDPLTHAALGDASE VEIDELQNQKEAEEPGPDSENSQENPPLRSSSS TTASSSPSTVIHGANSEPADSTEMDDKAAVGVS KPLPSVPPSIGKSNVDRRQAEIGEGAQKLLRPNS LRLASDSDAESDSRASSPNSTVSNTSTEGFGGI MSFASSLYRNHSTSFSLSNLTLPTKGAREKATP FPSLKGNRRALVDQKSSVIKHSPTVKREPPSPQG RSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKV RRLLESEQLRVFVLSKLNRMVQSEDDARQDIIP DVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGG MASIFGLLEIAQTHYYSKEPDKRKRSPTESVNT PVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSAT GKGPKELDTRSLKEENFIASIGPEVIKPVFDLGE TEEKKSQISADSGVSLTSSSQRTDQDSVIGVSP AVMIRSSSQDSEVSTVVSNSSGETLGADSDLSS NAGDGPGGEGSVHLASSRGTLSDSEIETNSATST IFGKAHSLKPSIKEKLAGSPIRTSEDVSQRVYL YEGLLGKERSTLWDQMQFWEDAFLDAVMLEREG MGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLA TLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHI GLVYSQQINEVLDQLANLNGRDLSIWSSGSRHM KKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIG TVYERWWYEKLINMTYCPKTKVLCLWRRNGSET QLNKFYTKKCRELYYCVKDSMERAAARQQSIKP GPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMH NQVFIELNHIKKCNTVRGVFVLEEFVPEIKEVV SHKYKTPMAHEICYSVLCLFSYVAAVHSSEEDL RTPPRPVSS*WRGATQLPQPRARPWPLAVPKCTM LL*LRSG*CSCVLCKPPAVAWLVTGYVSL*VCL ERVHLLPLHSQKTKLPSPQGSRMCTVCGAGVNP LFCVLETFVLWFLVLVPGVITVHCKSLALPSL*P GMTGRLEQFHSVRSEGTLGLTLSEEGHREEGKN WGAAGVSGSLPASFCIPKPAATAQGPQCWLLPA TASVTAVERRIPCGLPCLRHQRMEWSRLVESQGP LAALPEHQGGGRKDQAGFAHLSAGCLSRHGVSG GRDSLGMGKK*L*IFQPHIIYRKCTVV*M*NKC PQL

Sequence NM_130470

CACGTGCATGTGTAGCATGCCTTGGTTTTTCCTTTGGCATCTGAAAAAGGCACAACCTGAAAGACCTAGAACCCAGTGTCGGTCCCCAGGCCCTTTGGGA CAGGAAGAGAAGAGCCGTGTGGCCGCGGGGAGGATGTCCTGAGGCGGGGCTGTCCTCGCGGACTGACTGGACTCCATCTCCCAGCGGGCGCCGCGGCGCG GCCACGCCCCCCCACTCCCCGCGCGCGCCCGGTGGAGACTTCGATTTTCAGAATTCCTCCTGGGAATGCTGACTCCTTGCTTGGTGCCCTGATGCTTCTC TGAGATAAACTGATGAATTGGAACCATGGTGCAAAAGAAGAAGTTCTGTCCTCGGTTACTTGACTATCTAGTGATCGTAGGGGCCAGGCACCCGAGCAGT GATAGCGTGGCCCAGACTCCTGAATTGCTACGGCGATACCCCTTGGAGGATCACACTGAGTTTCCCCTGCCCCCAGATGTAGTGTTCTTCTGCCAGCCCG AGGGCTGCCTGAGCGTGCGGCAGCGGCGCATGAGCCTTCGGGATGATACCTCTTTTGTCTTCACCCTCACTGACAAGGACACTGGAGTCACGCGATATGG CATCTGTGTTAACTTCTACCGCTCCTTCCAAAAGCGAATCTCTAAGGAGAAGGGGGAAGGTGGGGCAGGGTCCCGTGGGAAGGAAGGAACCCATGCCACC TGTGCCTCAGAAGAGGGTGGCACTGAGAGCTCAGAGAGTGGCTCATCCCTGCAGCCTCTCAGTGCTGACTCTACCCCTGATGTGAACCAGTCTCCTCGGG GCAAACGCCGGGCCAAGGCGGGGAGCCGCTCCCGCAACAGTACTCTCACGTCCCTGTGCGTGCTCAGCCACTACCCTTTCTTCTCCACCTTCCGAGAGTG TTTGTATACTCTCAAGCGCCTGGTGGACTGCTGTAGTGAGCGCCTTCTGGGCAAGAAACTGGGCATCCCTCGAGGCGTACAAAGGGACACCATGTGGCGG ATCTTTACTGGATCGCTGCTGGTAGAGGAGAAGTCAAGTGCCCTTCTGCATGACCTTCGAGAGATTGAGGCCTGGATCTATCGATTGCTGCGCTCCCCAG TACCCGTCTCTGGGCAGAAGCGAGTAGACATCGAGGTCCTACCCCAAGAGCTCCAGCCAGCTCTGACCTTTGCTCTTCCAGACCCATCTCGATTCACCCT AGTGGATTTCCCACTGCACCTTCCCTTGGAACTTCTAGGTGTGGACGCCTGTCTCCAGGTGCTAACCTGCATTCTGTTAGAGCACAAGGTGGTGCTACAG TCCCGAGACTACAATGCACTCTCCATGTCTGTGATGGCATTCGTGGCAATGATCTACCCACTGGAGTATATGTTTCCTGTCATCCCGCTGCTACCCACCT GCATGGCATCAGCAGAGCAGCTGCTGTTGGCTCCAACCCCGTACATCATTGGGGTTCCTGCCAGCTTCTTCCTCTACAAACTGGACTTCAAAATGCCTGA TGATGTATGGCTAGTGGATCTGGACAGCAATAGGGTGATTGCCCCCACCAATGCAGAAGTGCTGCCTATCCTGCCAGAACCAGAATCACTAGAGCTGAAA AAGCATTTAAAGCAGGCCTTGGCCAGCATGAGTCTCAACACCCAGCCCATCCTCAATCTGGAGAAATTTCATGAGGGCCAGGAGATCCCCCTTCTCTTGG GAAGGCCTTCTAATGACCTGCAGTCCACACCGTCCACTGAATTCAACCCACTCATCTATGGCAATGATGTGGATTCTGTGGATGTTGCAACCAGGGTTGC CATGGTACGGTTCTTCAATTCCGCCAACGTGCTGCAGGGATTTCAGATGCACACGCGTACCCTGCGCCTCTTTCCTCGGCCTGTGGTAGCTTTTCAAGCT GGCTCCTTTCTAGCCTCACGTCCCCGGCAGACTCCTTTTGCCGAGAAATTGGCCAGGACTCAGGCTGTGGAGTACTTTGGGGAATGGATCCTTAACCCCA CCAACTATGCCTTTCAGCGAATTCACAACAATATGTTTGATCCAGCCCTGATTGGTGACAAGCCAAAGTGGTATGCTCATCAGCTGCAGCCTATCCACTA TCGCGTCTATGACAGCAATTCCCAGCTGGCTGAGGCCCTGAGTGTACCACCAGAGCGGGACTCTGACTCCGAACCTACTGATGATAGTGGCAGTGATAGT ATGGATTATGACGATTCAAGCTCTTCTTACTCCTCCCTTGGTGACTTTGTCAGTGAAATGATGAAATGTGACATTAATGGTGATACTCCCAATGTGGACC CTCTGACACATGCAGCACTGGGGGATGCCAGCGAGGTGGAGATTGACGAGCTGCAGAATCAGAAGGAAGCAGAAGAGCCTGGCCCAGACAGTGAGAACTC TCAGGAAAACCCCCCACTGCGCTCCAGCTCTAGCACCACAGCCAGCAGCAGCCCCAGCACTGTCATCCACGGAGCCAACTCTGAACCTGCTGACTCTACG GAGATGGATGATAAGGCAGCAGTAGGCGTCTCCAAGCCCCTCCCTTCCGTGCCTCCCAGCATTGGCAAATCGAACGTGGACAGACGTCAGGCAGAAATTG GAGAGGGGTCAGTGCGCCGGCGAATCTATGACAATCCATACTTCGAGCCCCAATATGGCTTTCCCCCTGAGGAAGATGAGGATGAGCAGGGGGAAAGTTA CACTCCCCGATTCAGCCAACATGTCAGTGGCAATCGGGCTCAAAAGCTGCTGCGGCCCAACAGCTTGAGACTGGCAAGTGACTCAGATGCAGAGTCAGAC TCTCGGGCAAGCTCTCCCAACTCCACCGTCTCCAACACCAGCACCGAGGGCTTCGGGGGCATCATGTCTTTTGCCAGCAGCCTCTATCGGAACCACAGTA CCAGCTTCAGTCTTTCAAACCTCACACTGCCCACCAAAGGTGCCCGAGAGAAGGCCACGCCCTTCCCCAGTCTGAAAGGAAACAGGAGGGCGTTAGTGGA TCAGAAGTCATCTGTCATTAAACACAGCCCAACAGTGAAAAGAGAACCTCCATCACCCCAGGGTCGATCCAGCAATTCTAGTGAGAACCAGCAGTTCCTG AAGGAGGTGGTGCACAGCGTGCTGGACGGCCAGGGAGTTGGCTGGCTCAACATGAAAAAGGTGCGCCGGCTGCTGGAGAGCGAGCAGCTGCGAGTCTTTG TCCTGAGCAAGCTGAACCGCATGGTGCAGTCAGAGGACGATGCCCGGCAGGACATCATCCCGGATGTGGAGATCAGTCGGAAGGTGTACAAGGGAATGTT AGACCTCCTCAAGTGTACAGTCCTCAGCTTGGAGCAGTCCTATGCCCACGCGGGTCTGGGTGGCATGGCCAGCATCTTTGGGCTTTTGGAGATTGCCCAG ACCCACTACTATAGTAAAGAACCAGACAAGCGGAAGAGAAGTCCAACAGAAAGTGTAAATACCCCAGTTGGCAAGGATCCTGGCCTAGCTGGGCGGGGGG ACCCAAAGGCTATGGCACAACTGAGAGTTCCACAACTGGGACCTCGGGCACCAAGTGCCACAGGAAAGGGTCCTAAGGAACTGGACACCAGAAGTTTAAA GGAAGAAAATTTTATAGCATCTATTGGGCCTGAAGTAATCAAACCTGTCTTTGACCTTGGTGAGACAGAGGAGAAAAAGTCCCAGATCAGCGCAGACAGT GGTGTGAGCCTGACGTCTAGTTCCCAGAGGACTGATCAAGACTCTGTCATCGGCGTGAGTCCAGCTGTTATGATCCGCAGCTCAAGTCAGGATTCTGAAG TTAGCACCGTGGTGAGTAATAGCTCTGGAGAGACCCTTGGAGCTGACAGTGACTTGAGCAGCAATGCAGGTGATGGACCAGGTGGCGAGGGCAGTGTTCA CCTGGCAAGCTCTCGGGGCACTTTGTCTGATAGTGAAATTGAGACCAACTCTGCCACAAGCACCATCTTTGGTAAAGCCCACAGCTTGAAGCCAAGCATA AAGGAGAAGCTGGCAGGCAGCCCCATTCGTACTTCTGAAGATGTGAGCCAGCGAGTCTATCTCTATGAGGGACTCCTAGGCAAAGAGCGTTCTACTTTAT GGGACCAAATGCAATTCTGGGAAGATGCCTTCTTAGATGCTGTGATGTTGGAGAGAGAAGGGATGGGTATGGACCAGGGTCCCCAGGAAATGATCGACAG GTACCTGTCCCTTGGAGAACATGACCGGAAGCGCCTGGAAGATGATGAAGATCGCTTGTTGGCCACACTTCTGCACAACCTCATCTCCTACATGCTGCTG ATGAAGGTAAATAAGAATGACATCCGCAAGAAGGTGAGGCGCCTAATGGGAAAGTCGCACATTGGGCTTGTGTACAGCCAGCAAATCAATGAGGTGCTTG ATCAGCTGGCGAACCTGAATGGACGCGATCTCTCTATCTGGTCCAGTGGCAGCCGGCACATGAAGAAGCAGACATTTGTGGTACATGCAGGGACAGATAC AAACGGAGATATCTTTTTCATGGAGGTGTGCGATGACTGTGTGGTGTTGCGTAGTAACATCGGAACAGTGTATGAGCGCTGGTGGTACGAGAAGCTCATC AACATGACCTACTGTCCCAAGACGAAGGTGTTGTGCTTGTGGCGTAGAAATGGCTCTGAGACCCAGCTCAACAAGTTCTATACTAAAAAGTGTCGGGAGC TGTACTACTGTGTGAAGGACAGCATGGAGCGCGCTGCCGCCCGACAGCAAAGCATCAAACCCGGACCTGAATTGGGTGGCGAGTTCCCTGTGCAGGACCT GAAGACTGGTGAGGGTGGCCTGCTGCAGGTGACCCTGGAAGGGATCAACCTCAAATTCATGCACAATCAGGTTTTCATAGAGCTGAATCACATTAAAAAG TGCAATACAGTTCGAGGCGTCTTTGTCCTGGAGGAATTTGTTCCTGAAATTAAAGAAGTGGTGAGCCACAAGTACAAGACACCAATGGCCCACGAAATCT GCTACTCCGTATTATGTCTCTTCTCGTACGTGGCTGCAGTTCATAGCAGTGAGGAAGATCTCAGAACCCCGCCCCGGCCTGTCTCTAGCTGATGGAGAGG GGCTACGCAGCTGCCCCAGCCCAGGGCACGCCCCTGGCCCCTTGCTGTTCCCAAGTGCACGATGCTGCTGTGACTGAGGAGTGGATGATGCTCGTGTGTC CTCTGCAAGCCCCCTGCTGTGGCTTGGTTGGTTACCGGTTATGTGTCCCTCTGAGTGTGTCTTGAGCGTGTCCACCTTCTCCCTCTCCACTCCCAGAAGA CCAAACTGCCTTCCCCTCAGGGCTCAAGAATGTGTACAGTCTGTGGGGCCGGTGTGAACCCACTATTTTGTGTCCTTGAGACATTTGTGTTGTGGTTCCT TGTCCTTGTCCCTGGCGTTATAACTGTCCACTGCAAGAGTCTGGCTCTCCCTTCTCTGTGACCCGGCATGACTGGGCGCCTGGAGCAGTTTCACTCTGTG AGGAGTGAGGGAACCCTGGGGCTCACCCTCTCAGAGGAAGGGCACAGAGAGGAAGGGAAGAATTGGGGGGCAGCCGGAGTGAGTGGCAGCCTCCCTGCTT CCTTCTGCATTCCCAAGCCGGCAGCTACTGCCCAGGGCCCGCAGTGTTGGCTGCTGCCTGCCACAGCCTCTGTGACTGCAGTGGAGCGGCGAATTCCCTG TGGCCTGCCATGCCTTCGGCATCAGAGGATGGAGTGGTCGAGGCTAGTGGAGTCCCAGGGACCGCTGGCTGCTCTGCCTGAGCATCAGGGAGGGGGCAGG AAAGACCAAGCTGGGTTTGCACATCTGTCTGCAGGCTGTCTCTCCAGGCACGGGGTGTCAGGAGGGAGAGACAGCCTGGGTATGGGCAAGAAATGACTGT AAATATTTCAGCCCCACATTATTTATAGAAAATGTACAGTTGTGTGAATGTGAAATAAATGTCCTCAACTCCC

refseq_MADD.F8 (forward primer)
refseq_MADD.R8 (reverse primer)
Single exon skipping

Frame 0

HVHV*HALVFPLASEKGTT*KT*NPVSVPRPFG TGREEPCGRGEDVLRRGCPRGLTGLHLPAGAAAR PRPPTPRARPVETSIFRIPPGNADSLLGALMLL *DKLMNWNHGAKEEVLSSVT*LSSDRRGQAPEQ **RGPDS*IATAIPLGGSH*VSPAPRCSVLLPAR GLPERAAAAHEPSG*YLFCLHPH*QGHWSHAIW HLC*LLPLLPKANL*GEGGRWGRVPWEGRNPCH LCLRRGWH*ELREWLIPAASQC*LYP*CEPVSSG QTPGQGGEPLPQQYSHVPVRAQPLPFLLHLPRV FVYSQAPGGLL**APSGQETGHPSRRTKGHHVA DLYWIAAGRGEVKCPSA*PSRD*GLDLSIAALPS TRLWAEASRHRGPTPRAPASSDLCSSRPISIHP SGFPTAPSLGTSRCGRLSPGANLHSVRAQGGAT VPRLQCTLHVCDGIRGNDLPTGVYVSCHPAATHL HGISRAAAVGSNPVHHWGSCQLLPLQTGLQNA* *CMASGSGQQ*GDCPHQCRSAAYPARTRITRAE KAFKAGLGQHESQHPAHPQSGEIS*GPGDPPSLG KAF**PAVHTVH*IQPTHLWQ*CGFCGCCNQGC HGTVLQFRQRAAGISDAHAYPAPLSSACGSFSS WLLSSLTSPADSFCREIGQDSGCGVLWGMDP*PH QLCLSANSQQYV*SSPDW*QAKVVCSSAAAYPL SRL*QQFPAG*GPECTTRAGL*LRTY***WQ** YGL*RFKLFLLLPW*LCQ*NDEM*H*W*YSQCGP SDTCSTGGCQRGGD*RAAESEGSRRAWPRQ*EL SGKPPTALQL*HHSQQQPQHCHPRSQL*TC*LY GDG**GSSRRLQAPPFRASQHWQIERGQTSGRNW RGVSAPANL*QSILRAPIWLSP*GR*G*AGGKL HSPIQPTCQWQSGSKAAAAQQLETGK*LRCRVR LSGKLSQLHRLQHQHRGLRGHHVFCQQPLSEPQY QLQSFKPHTAHQRCPREGHALPQSERKQEGVSG SEVICH*TQPNSEKRTSITPGSIQQF**EPAVP EGGGAQRAGRPGSWLAQHEKGAPAAGERAAASLC PEQAEPHGAVRGRCPAGHHPGCGDQSEGVQGNV RPPQVYSPQLGAVLCPRGSGWHGQHLWAFGDCP DPLL**RTRQAEEKSNRKCKYPSWQGSWPSWAGG PKGYGTTESSTTGTSGTKCHRKGS*GTGHQKFK GRKFYSIYWA*SNQTCL*PW*DRGEKVPDQRRQ WCEPDV*FPED*SRLCHRRESSCYDPQLKSGF*S *HRGE**LWRDPWS*Q*LEQQCR*WTRWRGQCS PGKLSGHFV***N*DQLCHKHHLW*SPQLEAKH KGEAGRQPHSYF*RCEPASLSL*GTPRQRAFYFM GPNAILGRCLLRCCDVGERRDGYGPGSPGNDRQ VPVPWRT*PEAPGR**RSLVGHTSAQPHLLHAA DEGK*E*HPQEGEAPNGKVAHWACVQPANQ*GA* SAGEPEWTRSLYLVQWQPAHEEADICGTCRDRY KRRYLFHGGVR*LCGVA**HRNSV*ALVVREAH QHDLLSQDEGVVLVA*KWL*DPAQQVLY*KVSGA VLLCEGQHGARCRPTAKHQTRT*IGWRVPCAGP EDW*GWPAAGDPGRDQPQIHAQSGFHRAESH*K VQYSSRRLCPGGICS*N*RSGEPQVQDTNGPRNL LLRIMSLLVRGCSS*Q*GRSQNPAPACL*LMER GYAAAPAQGTPLAPCCSQVHDAAVTEEWMMLVC PLQAPCCGLVGYRLCVPLSVS*ACPPSPSPLPED QTAFPSGLKNVYSLWGRCEPTILCP*DICVVVP CPCPWRYNCPLQESGSPFSVTRHDWAPGAVSLC EE*GNPGAHPLRGRAQRGREELGGSRSEWQPPCF LLHSQAGSYCPGPAVLAAACHSLCDCSGAANSL WPAMPSASEDGVVEASGVPGTAGCSA*ASGRGQ ERPSWVCTSVCRLSLQARGVRRERQPGYGQEMTV NISAPHYL*KMYSCVNVK*MSSTP

Frame 1

TCMCSMPWFFLWHLKKAQPERPRTQCRSPGPLG QEEKSRVAAGRMS*GGAVLAD*LDSISQRAPRR GHAPPLPARARWRLRFSEFLLGMLTPCLVP*CFS EIN**IGTMVQKKKFCPRLLDYLVIVGARHPSS DSVAQTPELLRRYPLEDHTEFPLPPDVVFFCQP EGCLSVRQRRMSLRDDTSFVFTLTDKDTGVTRYG ICVNFYRSFQKRISKEKGEGGAGSRGKEGTHAT CASEEGGTESSESGSSLQPLSADSTPDVNQSPR GKRRAKAGSRSRNSTLTSLCVLSHYPFFSTFREC LYTLKRLVDCCSERLLGKKLGIPRGVQRDTMWR IFTGSLLVEEKSSALLHDLREIEAWIYRLLRSP VPVSGQKRVDIEVLPQELQPALTFALPDPSRFTL VDFPLHLPLELLGVDACLQVLTCILLEHKVVLQ SRDYNALSMSVMAFVAMIYPLEYMFPVIPLLPT CMASAEQLLLAPTPYIIGVPASFFLYKLDFKMPD DVWLVDLDSNRVIAPTNAEVLPILPEPESLELK KHLKQALASMSLNTQPILNLEKFHEGQEIPLLL GRPSNDLQSTPSTEFNPLIYGNDVDSVDVATRVA MVRFFNSANVLQGFQMHTRTLRLFPRPVVAFQA GSFLASRPRQTPFAEKLARTQAVEYFGEWILNP TNYAFQRIHNNMFDPALIGDKPKWYAHQLQPIHY RVYDSNSQLAEALSVPPERDSDSEPTDDSGSDS MDYDDSSSSYSSLGDFVSEMMKCDINGDTPNVD PLTHAALGDASEVEIDELQNQKEAEEPGPDSENS QENPPLRSSSSTTASSSPSTVIHGANSEPADST EMDDKAAVGVSKPLPSVPPSIGKSNVDRRQAEI GEGSVRRRIYDNPYFEPQYGFPPEEDEDEQGESY TPRFSQHVSGNRAQKLLRPNSLRLASDSDAESD SRASSPNSTVSNTSTEGFGGIMSFASSLYRNHS TSFSLSNLTLPTKGAREKATPFPSLKGNRRALVD QKSSVIKHSPTVKREPPSPQGRSSNSSENQQFL KEVVHSVLDGQGVGWLNMKKVRRLLESEQLRVF VLSKLNRMVQSEDDARQDIIPDVEISRKVYKGML DLLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQ THYYSKEPDKRKRSPTESVNTPVGKDPGLAGRG DPKAMAQLRVPQLGPRAPSATGKGPKELDTRSLK EENFIASIGPEVIKPVFDLGETEEKKSQISADS GVSLTSSSQRTDQDSVIGVSPAVMIRSSSQDSE VSTVVSNSSGETLGADSDLSSNAGDGPGGEGSVH LASSRGTLSDSEIETNSATSTIFGKAHSLKPSI KEKLAGSPIRTSEDVSQRVYLYEGLLGKERSTL WDQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDR YLSLGEHDRKRLEDDEDRLLATLLHNLISYMLL MKVNKNDIRKKVRRLMGKSHIGLVYSQQINEVL DQLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDT NGDIFFMEVCDDCVVLRSNIGTVYERWWYEKLI NMTYCPKTKVLCLWRRNGSETQLNKFYTKKCRE LYYCVKDSMERAAARQQSIKPGPELGGEFPVQDL KTGEGGLLQVTLEGINLKFMHNQVFIELNHIKK CNTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEI CYSVLCLFSYVAAVHSSEEDLRTPPRPVSS*WRG ATQLPQPRARPWPLAVPKCTMLL*LRSG*CSCV LCKPPAVAWLVTGYVSL*VCLERVHLLPLHSQK TKLPSPQGSRMCTVCGAGVNPLFCVLETFVLWFL VLVPGVITVHCKSLALPSL*PGMTGRLEQFHSV RSEGTLGLTLSEEGHREEGKNWGAAGVSGSLPA SFCIPKPAATAQGPQCWLLPATASVTAVERRIPC GLPCLRHQRMEWSRLVESQGPLAALPEHQGGGR KDQAGFAHLSAGCLSRHGVSGGRDSLGMGKK*L *IFQPHIIYRKCTVV*M*NKCPQL

Frame 2

RACVACLGFSFGI*KRHNLKDLEPSVGPQALWD RKRRAVWPRGGCPEAGLSSRTDWTPSPSGRRGA ATPPHSPRAPGGDFDFQNSSWEC*LLAWCPDAS LR*TDELEPWCKRRSSVLGYLTI**S*GPGTRAV IAWPRLLNCYGDTPWRITLSFPCPQM*CSSASP RAA*ACGSGA*AFGMIPLLSSPSLTRTLESRDM ASVLTSTAPSKSESLRRRGKVGQGPVGRKEPMPP VPQKRVALRAQRVAHPCSLSVLTLPLM*TSLLG ANAGPRRGAAPATVLSRPCACSATTLSSPPSES VCILSSAWWTAVVSAFWARNWASLEAYKGTPCGG SLLDRCW*RRSQVPFCMTFERLRPGSIDCCAPQ YPSLGRSE*TSRSYPKSSSQL*PLLFQTHLDSP *WISHCTFPWNF*VWTPVSRC*PAFC*STRWCYS PETTMHSPCL*WHSWQ*STHWSICFLSSRCYPP AWHQQSSCCWLQPRTSLGFLPASSSTNWTSKCL MMYG*WIWTAIG*LPPPMQKCCLSCQNQNH*S*K SI*SRPWPA*VSTPSPSSIWRNFMRARRSPFSW EGLLMTCSPHRPLNSTHSSMAMMWILWMLQPGL PWYGSSIPPTCCRDFRCTRVPCASFLGLW*LFKL APF*PHVPGRLLLPRNWPGLRLWSTLGNGSLTP PTMPFSEFTTICLIQP*LVTSQSGMLISCSLST IASMTAIPSWLRP*VYHQSGTLTPNLLMIVAVIV WIMTIQALLTPPLVTLSVK**NVTLMVILPMWT L*HMQHWGMPARWRLTSCRIRRKQKSLAQTVRT LRKTPHCAPALAPQPAAAPALSSTEPTLNLLTLR RWMIRQQ*ASPSPSLPCLPALANRTWTDVRQKL ERGQCAGESMTIHTSSPNMAFPLRKMRMSRGKV TLPDSANMSVAIGLKSCCGPTA*DWQVTQMQSQT LGQALPTPPSPTPAPRASGASCLLPAASIGTTV PASVFQTSHCPPKVPERRPRPSPV*KETGGR*W IRSHLSLNTAQQ*KENLHHPRVDPAILVRTSSS* RRWCTACWTARELAGST*KRCAGCWRASSCESL S*AS*TAWCSQRTMPGRTSSRMWRSVGRCTREC *TSSSVQSSAWSSPMPTRVWVAWPASLGFWRLPR PTTIVKNQTSGREVQQKV*IPQLARILA*LGGG TQRLWHN*EFHNWDLGHQVPQERVLRNWTPEV* RKKIL*HLLGLK*SNLSLTLVRQRRKSPRSAQTV V*A*RLVPRGLIKTLSSA*VQLL*SAAQVRILK LAPW*VIALERPLELTVT*AAMQVMDQVARAVF TWQALGALCLIVKLRPTLPQAPSLVKPTA*SQA* RRSWQAAPFVLLKM*ASESISMRDS*AKSVLLY GTKCNSGKMPS*ML*CWREKGWVWTRVPRK*ST GTCPLENMTGSAWKMMKIACWPHFCTTSSPTCC* *R*IRMTSARR*GA*WESRTLGLCTASKSMRCL ISWRT*MDAISLSGPVAAGT*RSRHLWYMQGQI QTEISFSWRCAMTVWCCVVTSEQCMSAGGTRSSS T*PTVPRRRCCACGVEMALRPSSTSSILKSVGS CTTV*RTAWSALPPDSKASNPDLNWVASSLCRT *RLVRVACCR*PWKGSTSNSCTIRFS*S*ITLKS AIQFEASLSWRNLFLKLKKW*ATSTRHQWPTKS ATPYYVSSRTWLQFIAVRKISEPRPGLSLADGE GLRSCPSPGHAPGPLLFPSARCCCD*GVDDARVS SASPLLWLGWLPVMCPSECVLSVSTFSLSTPRR PNCLPLRAQECVQSVGPV*THYFVSLRHLCCGS LSLSLAL*LSTARVWLSLLCDPA*LGAWSSFTL* GVREPWGSPSQRKGTERKGRIGGQPE*VAASLL PSAFPSRQLLPRARSVGCCLPQPL*LQWSGEFP VACHAFGIRGWSGRG*WSPRDRWLLCLSIREGAG KTKLGLHICLQAVSPGTGCQEGETAWVWARNDC KYFSPTLFIENVQLCECEINVLNS

Sequence NM_130474

ACCGCGGCCGCGCGCCCCCCTCTGCCCCCGCTGCCCAGGATTGGTAGGCTCCACCGCTCGGCAGCCGGCTTCCCTGCTCGGACGCCGAGCACCGCCAAAG CGCACTTCGATTTTCAGAATTCCTCCTGGGAATGCTGACTCCTTGCTTGGTGCCCTGATGCTTCTCTGAGATAAACTGATGAATTGGAACCATGGTGCAA AAGAAGAAGTTCTGTCCTCGGTTACTTGACTATCTAGTGATCGTAGGGGCCAGGCACCCGAGCAGTGATAGCGTGGCCCAGACTCCTGAATTGCTACGGC GATACCCCTTGGAGGATCACACTGAGTTTCCCCTGCCCCCAGATGTAGTGTTCTTCTGCCAGCCCGAGGGCTGCCTGAGCGTGCGGCAGCGGCGCATGAG CCTTCGGGATGATACCTCTTTTGTCTTCACCCTCACTGACAAGGACACTGGAGTCACGCGATATGGCATCTGTGTTAACTTCTACCGCTCCTTCCAAAAG CGAATCTCTAAGGAGAAGGGGGAAGGTGGGGCAGGGTCCCGTGGGAAGGAAGGAACCCATGCCACCTGTGCCTCAGAAGAGGGTGGCACTGAGAGCTCAG AGAGTGGCTCATCCCTGCAGCCTCTCAGTGCTGACTCTACCCCTGATGTGAACCAGTCTCCTCGGGGCAAACGCCGGGCCAAGGCGGGGAGCCGCTCCCG CAACAGTACTCTCACGTCCCTGTGCGTGCTCAGCCACTACCCTTTCTTCTCCACCTTCCGAGAGTGTTTGTATACTCTCAAGCGCCTGGTGGACTGCTGT AGTGAGCGCCTTCTGGGCAAGAAACTGGGCATCCCTCGAGGCGTACAAAGGGACACCATGTGGCGGATCTTTACTGGATCGCTGCTGGTAGAGGAGAAGT CAAGTGCCCTTCTGCATGACCTTCGAGAGATTGAGGCCTGGATCTATCGATTGCTGCGCTCCCCAGTACCCGTCTCTGGGCAGAAGCGAGTAGACATCGA GGTCCTACCCCAAGAGCTCCAGCCAGCTCTGACCTTTGCTCTTCCAGACCCATCTCGATTCACCCTAGTGGATTTCCCACTGCACCTTCCCTTGGAACTT CTAGGTGTGGACGCCTGTCTCCAGGTGCTAACCTGCATTCTGTTAGAGCACAAGGTGGTGCTACAGTCCCGAGACTACAATGCACTCTCCATGTCTGTGA TGGCATTCGTGGCAATGATCTACCCACTGGAGTATATGTTTCCTGTCATCCCGCTGCTACCCACCTGCATGGCATCAGCAGAGCAGCTGCTGTTGGCTCC AACCCCGTACATCATTGGGGTTCCTGCCAGCTTCTTCCTCTACAAACTGGACTTCAAAATGCCTGATGATGTATGGCTAGTGGATCTGGACAGCAATAGG GTGATTGCCCCCACCAATGCAGAAGTGCTGCCTATCCTGCCAGAACCAGAATCACTAGAGCTGAAAAAGCATTTAAAGCAGGCCTTGGCCAGCATGAGTC TCAACACCCAGCCCATCCTCAATCTGGAGAAATTTCATGAGGGCCAGGAGATCCCCCTTCTCTTGGGAAGGCCTTCTAATGACCTGCAGTCCACACCGTC CACTGAATTCAACCCACTCATCTATGGCAATGATGTGGATTCTGTGGATGTTGCAACCAGGGTTGCCATGGTACGGTTCTTCAATTCCGCCAACGTGCTG CAGGGATTTCAGATGCACACGCGTACCCTGCGCCTCTTTCCTCGGCCTGTGGTAGCTTTTCAAGCTGGCTCCTTTCTAGCCTCACGTCCCCGGCAGACTC CTTTTGCCGAGAAATTGGCCAGGACTCAGGCTGTGGAGTACTTTGGGGAATGGATCCTTAACCCCACCAACTATGCCTTTCAGCGAATTCACAACAATAT GTTTGATCCAGCCCTGATTGGTGACAAGCCAAAGTGGTATGCTCATCAGCTGCAGCCTATCCACTATCGCGTCTATGACAGCAATTCCCAGCTGGCTGAG GCCCTGAGTGTACCACCAGAGCGGGACTCTGACTCCGAACCTACTGATGATAGTGGCAGTGATAGTATGGATTATGACGATTCAAGCTCTTCTTACTCCT CCCTTGGTGACTTTGTCAGTGAAATGATGAAATGTGACATTAATGGTGATACTCCCAATGTGGACCCTCTGACACATGCAGCACTGGGGGATGCCAGCGA GGTGGAGATTGACGAGCTGCAGAATCAGAAGGAAGCAGAAGAGCCTGGCCCAGACAGTGAGAACTCTCAGGAAAACCCCCCACTGCGCTCCAGCTCTAGC ACCACAGCCAGCAGCAGCCCCAGCACTGTCATCCACGGAGCCAACTCTGAACCTGCTGACTCTACGGAGATGGATGATAAGGCAGCAGTAGGCGTCTCCA AGCCCCTCCCTTCCGTGCCTCCCAGCATTGGCAAATCGAACGTGGACAGACGTCAGGCAGAAATTGGAGAGGGGGCTCAAAAGCTGCTGCGGCCCAACAG CTTGAGACTGGCAAGTGACTCAGATGCAGAGTCAGACTCTCGGGCAAGCTCTCCCAACTCCACCGTCTCCAACACCAGCACCGAGGGCTTCGGGGGCATC ATGTCTTTTGCCAGCAGCCTCTATCGGAACCACAGTACCAGCTTCAGTCTTTCAAACCTCACACTGCCCACCAAAGGTGCCCGAGAGAAGGCCACGCCCT TCCCCAGTCTGAAAGGAAACAGGAGGGCGTTAGTGGATCAGAAGTCATCTGTCATTAAACACAGCCCAACAGTGAAAAGAGAACCTCCATCACCCCAGGG TCGATCCAGCAATTCTAGTGAGAACCAGCAGTTCCTGAAGGAGGTGGTGCACAGCGTGCTGGACGGCCAGGGAGTTGGCTGGCTCAACATGAAAAAGGTG CGCCGGCTGCTGGAGAGCGAGCAGCTGCGAGTCTTTGTCCTGAGCAAGCTGAACCGCATGGTGCAGTCAGAGGACGATGCCCGGCAGGACATCATCCCGG ATGTGGAGATCAGTCGGAAGGTGTACAAGGGAATGTTAGACCTCCTCAAGTGTACAGTCCTCAGCTTGGAGCAGTCCTATGCCCACGCGGGTCTGGGTGG CATGGCCAGCATCTTTGGGCTTTTGGAGATTGCCCAGACCCACTACTATAGTAAAGAACCAGACAAGCGGAAGAGAAGTCCAACAGAAAGTGTAAATACC CCAGTTGGCAAGGATCCTGGCCTAGCTGGGCGGGGGGACCCAAAGGCTATGGCACAACTGAGAGTTCCACAACTGGGACCTCGGGCACCAAGTGCCACAG GAAAGGGTCCTAAGGAACTGGACACCAGAAGTTTAAAGGAAGAAAATTTTATAGCATCTATTGGGCCTGAAGTAATCAAACCTGTCTTTGACCTTGGTGA GACAGAGGAGAAAAAGTCCCAGATCAGCGCAGACAGTGGTGTGAGCCTGACGTCTAGTTCCCAGAGGACTGATCAAGACTCTGTCATCGGCGTGAGTCCA GCTGTTATGATCCGCAGCTCAAGTCAGGATTCTGAAGTTAGCACCGTGGTGAGTAATAGCTCTGGAGAGACCCTTGGAGCTGACAGTGACTTGAGCAGCA ATGCAGGTGATGGACCAGGTGGCGAGGGCAGTGTTCACCTGGCAAGCTCTCGGGGCACTTTGTCTGATAGTGAAATTGAGACCAACTCTGCCACAAGCAC CATCTTTGGTAAAGCCCACAGCTTGAAGCCAAGCATAAAGGAGAAGCTGGCAGGCAGCCCCATTCGTACTTCTGAAGATGTGAGCCAGCGAGTCTATCTC TATGAGGGACTCCTAGGCAAAGAGCGTTCTACTTTATGGGACCAAATGCAATTCTGGGAAGATGCCTTCTTAGATGCTGTGATGTTGGAGAGAGAAGGGA TGGGTATGGACCAGGGTCCCCAGGAAATGATCGACAGGTACCTGTCCCTTGGAGAACATGACCGGAAGCGCCTGGAAGATGATGAAGATCGCTTGTTGGC CACACTTCTGCACAACCTCATCTCCTACATGCTGCTGATGAAGGTAAATAAGAATGACATCCGCAAGAAGGTGAGGCGCCTAATGGGAAAGTCGCACATT GGGCTTGTGTACAGCCAGCAAATCAATGAGGTGCTTGATCAGCTGGCGAACCTGAATGGACGCGATCTCTCTATCTGGTCCAGTGGCAGCCGGCACATGA AGAAGCAGACATTTGTGGTACATGCAGGGACAGATACAAACGGAGATATCTTTTTCATGGAGGTGTGCGATGACTGTGTGGTGTTGCGTAGTAACATCGG AACAGTGTATGAGCGCTGGTGGTACGAGAAGCTCATCAACATGACCTACTGTCCCAAGACGAAGGTGTTGTGCTTGTGGCGTAGAAATGGCTCTGAGACC CAGCTCAACAAGTTCTATACTAAAAAGTGTCGGGAGCTGTACTACTGTGTGAAGGACAGCATGGAGCGCGCTGCCGCCCGACAGCAAAGCATCAAACCCG GACCTGAATTGGGTGGCGAGTTCCCTGTGCAGGACCTGAAGACTGGTGAGGGTGGCCTGCTGCAGGTGACCCTGGAAGGGATCAACCTCAAATTCATGCA CAATCAGTTCCTGAAATTAAAGAAGTGGTGAGCCACAAGTACAAGACACCAATGGCCCACGAAATCTGCTACTCCGTATTATGTCTCTTCTCGTACGTGG CTGCAGTTCATAGCAGTGAGGAAGATCTCAGAACCCCGCCCCGGCCTGTCTCTAGCTGATGGAGAGGGGCTACGCAGCTGCCCCAGCCCAGGGCACGCCC CTGGCCCCTTGCTGTTCCCAAGTGCACGATGCTGCTGTGACTGAGGAGTGGATGATGCTCGTGTGTCCTCTGCAAGCCCCCTGCTGTGGCTTGGTTGGTT ACCGGTTATGTGTCCCTCTGAGTGTGTCTTGAGCGTGTCCACCTTCTCCCTCTCCACTCCCAGAAGACCAAACTGCCTTCCCCTCAGGGCTCAAGAATGT GTACAGTCTGTGGGGCCGGTGTGAACCCACTATTTTGTGTCCTTGAGACATTTGTGTTGTGGTTCCTTGTCCTTGTCCCTGGCGTTATAACTGTCCACTG CAAGAGTCTGGCTCTCCCTTCTCTGTGACCCGGCATGACTGGGCGCCTGGAGCAGTTTCACTCTGTGAGGAGTGAGGGAACCCTGGGGCTCACCCTCTCA GAGGAAGGGCACAGAGAGGAAGGGAAGAATTGGGGGGCAGCCGGAGTGAGTGGCAGCCTCCCTGCTTCCTTCTGCATTCCCAAGCCGGCAGCTACTGCCC AGGGCCCGCAGTGTTGGCTGCTGCCTGCCACAGCCTCTGTGACTGCAGTGGAGCGGCGAATTCCCTGTGGCCTGCCATGCCTTCGGCATCAGAGGATGGA GTGGTCGAGGCTAGTGGAGTCCCAGGGACCGCTGGCTGCTCTGCCTGAGCATCAGGGAGGGGGCAGGAAAGACCAAGCTGGGTTTGCACATCTGTCTGCA GGCTGTCTCTCCAGGCACGGGGTGTCAGGAGGGAGAGACAGCCTGGGTATGGGCAAGAAATGACTGTAAATATTTCAGCCCCACATTATTTATAGAAAAT GTACAGTTGTGTGAATGTGAAATAAATGTCCTCAACTCCC

refseq_MADD.F8 (forward primer)
refseq_MADD.R8 (reverse primer)
Single exon skipping

Frame 0

TAAARPPLPPLPRIGRLHRSAAGFPARTPSTAK AHFDFQNSSWEC*LLAWCPDASLR*TDELEPWCK RRSSVLGYLTI**S*GPGTRAVIAWPRLLNCYG DTPWRITLSFPCPQM*CSSASPRAA*ACGSGA* AFGMIPLLSSPSLTRTLESRDMASVLTSTAPSKS ESLRRRGKVGQGPVGRKEPMPPVPQKRVALRAQ RVAHPCSLSVLTLPLM*TSLLGANAGPRRGAAP ATVLSRPCACSATTLSSPPSESVCILSSAWWTAV VSAFWARNWASLEAYKGTPCGGSLLDRCW*RRS QVPFCMTFERLRPGSIDCCAPQYPSLGRSE*TS RSYPKSSSQL*PLLFQTHLDSP*WISHCTFPWNF *VWTPVSRC*PAFC*STRWCYSPETTMHSPCL* WHSWQ*STHWSICFLSSRCYPPAWHQQSSCCWL QPRTSLGFLPASSSTNWTSKCLMMYG*WIWTAIG *LPPPMQKCCLSCQNQNH*S*KSI*SRPWPA*V STPSPSSIWRNFMRARRSPFSWEGLLMTCSPHR PLNSTHSSMAMMWILWMLQPGLPWYGSSIPPTCC RDFRCTRVPCASFLGLW*LFKLAPF*PHVPGRL LLPRNWPGLRLWSTLGNGSLTPPTMPFSEFTTI CLIQP*LVTSQSGMLISCSLSTIASMTAIPSWLR P*VYHQSGTLTPNLLMIVAVIVWIMTIQALLTP PLVTLSVK**NVTLMVILPMWTL*HMQHWGMPA RWRLTSCRIRRKQKSLAQTVRTLRKTPHCAPALA PQPAAAPALSSTEPTLNLLTLRRWMIRQQ*ASP SPSLPCLPALANRTWTDVRQKLERGLKSCCGPT A*DWQVTQMQSQTLGQALPTPPSPTPAPRASGAS CLLPAASIGTTVPASVFQTSHCPPKVPERRPRP SPV*KETGGR*WIRSHLSLNTAQQ*KENLHHPR VDPAILVRTSSS*RRWCTACWTARELAGST*KRC AGCWRASSCESLS*AS*TAWCSQRTMPGRTSSR MWRSVGRCTREC*TSSSVQSSAWSSPMPTRVWV AWPASLGFWRLPRPTTIVKNQTSGREVQQKV*IP QLARILA*LGGGTQRLWHN*EFHNWDLGHQVPQ ERVLRNWTPEV*RKKIL*HLLGLK*SNLSLTLV RQRRKSPRSAQTVV*A*RLVPRGLIKTLSSA*VQ LL*SAAQVRILKLAPW*VIALERPLELTVT*AA MQVMDQVARAVFTWQALGALCLIVKLRPTLPQA PSLVKPTA*SQA*RRSWQAAPFVLLKM*ASESIS MRDS*AKSVLLYGTKCNSGKMPS*ML*CWREKG WVWTRVPRK*STGTCPLENMTGSAWKMMKIACW PHFCTTSSPTCC**R*IRMTSARR*GA*WESRTL GLCTASKSMRCLISWRT*MDAISLSGPVAAGT* RSRHLWYMQGQIQTEISFSWRCAMTVWCCVVTS EQCMSAGGTRSSST*PTVPRRRCCACGVEMALRP SSTSSILKSVGSCTTV*RTAWSALPPDSKASNP DLNWVASSLCRT*RLVRVACCR*PWKGSTSNSC TISS*N*RSGEPQVQDTNGPRNLLLRIMSLLVRG CSS*Q*GRSQNPAPACL*LMERGYAAAPAQGTP LAPCCSQVHDAAVTEEWMMLVCPLQAPCCGLVG YRLCVPLSVS*ACPPSPSPLPEDQTAFPSGLKNV YSLWGRCEPTILCP*DICVVVPCPCPWRYNCPL QESGSPFSVTRHDWAPGAVSLCEE*GNPGAHPL RGRAQRGREELGGSRSEWQPPCFLLHSQAGSYCP GPAVLAAACHSLCDCSGAANSLWPAMPSASEDG VVEASGVPGTAGCSA*ASGRGQERPSWVCTSVC RLSLQARGVRRERQPGYGQEMTVNISAPHYL*KM YSCVNVK*MSSTP

Frame 1

PRPRAPLCPRCPGLVGSTARQPASLLGRRAPPK RTSIFRIPPGNADSLLGALMLL*DKLMNWNHGA KEEVLSSVT*LSSDRRGQAPEQ**RGPDS*IATA IPLGGSH*VSPAPRCSVLLPARGLPERAAAAHE PSG*YLFCLHPH*QGHWSHAIWHLC*LLPLLPK ANL*GEGGRWGRVPWEGRNPCHLCLRRGWH*ELR EWLIPAASQC*LYP*CEPVSSGQTPGQGGEPLP QQYSHVPVRAQPLPFLLHLPRVFVYSQAPGGLL **APSGQETGHPSRRTKGHHVADLYWIAAGRGEV KCPSA*PSRD*GLDLSIAALPSTRLWAEASRHR GPTPRAPASSDLCSSRPISIHPSGFPTAPSLGT SRCGRLSPGANLHSVRAQGGATVPRLQCTLHVCD GIRGNDLPTGVYVSCHPAATHLHGISRAAAVGS NPVHHWGSCQLLPLQTGLQNA**CMASGSGQQ* GDCPHQCRSAAYPARTRITRAEKAFKAGLGQHES QHPAHPQSGEIS*GPGDPPSLGKAF**PAVHTV H*IQPTHLWQ*CGFCGCCNQGCHGTVLQFRQRA AGISDAHAYPAPLSSACGSFSSWLLSSLTSPADS FCREIGQDSGCGVLWGMDP*PHQLCLSANSQQY V*SSPDW*QAKVVCSSAAAYPLSRL*QQFPAG* GPECTTRAGL*LRTY***WQ**YGL*RFKLFLLL PW*LCQ*NDEM*H*W*YSQCGPSDTCSTGGCQR GGD*RAAESEGSRRAWPRQ*ELSGKPPTALQL* HHSQQQPQHCHPRSQL*TC*LYGDG**GSSRRLQ APPFRASQHWQIERGQTSGRNWRGGSKAAAAQQ LETGK*LRCRVRLSGKLSQLHRLQHQHRGLRGH HVFCQQPLSEPQYQLQSFKPHTAHQRCPREGHAL PQSERKQEGVSGSEVICH*TQPNSEKRTSITPG SIQQF**EPAVPEGGGAQRAGRPGSWLAQHEKG APAAGERAAASLCPEQAEPHGAVRGRCPAGHHPG CGDQSEGVQGNVRPPQVYSPQLGAVLCPRGSGW HGQHLWAFGDCPDPLL**RTRQAEEKSNRKCKY PSWQGSWPSWAGGPKGYGTTESSTTGTSGTKCHR KGS*GTGHQKFKGRKFYSIYWA*SNQTCL*PW* DRGEKVPDQRRQWCEPDV*FPED*SRLCHRRES SCYDPQLKSGF*S*HRGE**LWRDPWS*Q*LEQQ CR*WTRWRGQCSPGKLSGHFV***N*DQLCHKH HLW*SPQLEAKHKGEAGRQPHSYF*RCEPASLS L*GTPRQRAFYFMGPNAILGRCLLRCCDVGERRD GYGPGSPGNDRQVPVPWRT*PEAPGR**RSLVG HTSAQPHLLHAADEGK*E*HPQEGEAPNGKVAH WACVQPANQ*GA*SAGEPEWTRSLYLVQWQPAHE EADICGTCRDRYKRRYLFHGGVR*LCGVA**HR NSV*ALVVREAHQHDLLSQDEGVVLVA*KWL*D PAQQVLY*KVSGAVLLCEGQHGARCRPTAKHQTR T*IGWRVPCAGPEDW*GWPAAGDPGRDQPQIHA QSVPEIKEVVSHKYKTPMAHEICYSVLCLFSYV AAVHSSEEDLRTPPRPVSS*WRGATQLPQPRARP WPLAVPKCTMLL*LRSG*CSCVLCKPPAVAWLV TGYVSL*VCLERVHLLPLHSQKTKLPSPQGSRM CTVCGAGVNPLFCVLETFVLWFLVLVPGVITVHC KSLALPSL*PGMTGRLEQFHSVRSEGTLGLTLS EEGHREEGKNWGAAGVSGSLPASFCIPKPAATA QGPQCWLLPATASVTAVERRIPCGLPCLRHQRME WSRLVESQGPLAALPEHQGGGRKDQAGFAHLSA GCLSRHGVSGGRDSLGMGKK*L*IFQPHIIYRK CTVV*M*NKCPQL

Frame 2

RGRAPPSAPAAQDW*APPLGSRLPCSDAEHRQS ALRFSEFLLGMLTPCLVP*CFSEIN**IGTMVQ KKKFCPRLLDYLVIVGARHPSSDSVAQTPELLR RYPLEDHTEFPLPPDVVFFCQPEGCLSVRQRRMS LRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQK RISKEKGEGGAGSRGKEGTHATCASEEGGTESS ESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSR NSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCC SERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEK SSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIE VLPQELQPALTFALPDPSRFTLVDFPLHLPLEL LGVDACLQVLTCILLEHKVVLQSRDYNALSMSV MAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAP TPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNR VIAPTNAEVLPILPEPESLELKKHLKQALASMS LNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPS TEFNPLIYGNDVDSVDVATRVAMVRFFNSANVL QGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQT PFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNM FDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAE ALSVPPERDSDSEPTDDSGSDSMDYDDSSSSYS SLGDFVSEMMKCDINGDTPNVDPLTHAALGDASE VEIDELQNQKEAEEPGPDSENSQENPPLRSSSS TTASSSPSTVIHGANSEPADSTEMDDKAAVGVS KPLPSVPPSIGKSNVDRRQAEIGEGAQKLLRPNS LRLASDSDAESDSRASSPNSTVSNTSTEGFGGI MSFASSLYRNHSTSFSLSNLTLPTKGAREKATP FPSLKGNRRALVDQKSSVIKHSPTVKREPPSPQG RSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKV RRLLESEQLRVFVLSKLNRMVQSEDDARQDIIP DVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGG MASIFGLLEIAQTHYYSKEPDKRKRSPTESVNT PVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSAT GKGPKELDTRSLKEENFIASIGPEVIKPVFDLGE TEEKKSQISADSGVSLTSSSQRTDQDSVIGVSP AVMIRSSSQDSEVSTVVSNSSGETLGADSDLSS NAGDGPGGEGSVHLASSRGTLSDSEIETNSATST IFGKAHSLKPSIKEKLAGSPIRTSEDVSQRVYL YEGLLGKERSTLWDQMQFWEDAFLDAVMLEREG MGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRLLA TLLHNLISYMLLMKVNKNDIRKKVRRLMGKSHI GLVYSQQINEVLDQLANLNGRDLSIWSSGSRHM KKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSNIG TVYERWWYEKLINMTYCPKTKVLCLWRRNGSET QLNKFYTKKCRELYYCVKDSMERAAARQQSIKP GPELGGEFPVQDLKTGEGGLLQVTLEGINLKFMH NQFLKLKKW*ATSTRHQWPTKSATPYYVSSRTW LQFIAVRKISEPRPGLSLADGEGLRSCPSPGHA PGPLLFPSARCCCD*GVDDARVSSASPLLWLGWL PVMCPSECVLSVSTFSLSTPRRPNCLPLRAQEC VQSVGPV*THYFVSLRHLCCGSLSLSLAL*LST ARVWLSLLCDPA*LGAWSSFTL*GVREPWGSPSQ RKGTERKGRIGGQPE*VAASLLPSAFPSRQLLP RARSVGCCLPQPL*LQWSGEFPVACHAFGIRGW SGRG*WSPRDRWLLCLSIREGAGKTKLGLHICLQ AVSPGTGCQEGETAWVWARNDCKYFSPTLFIEN VQLCECEINVLNS

Sequence NM_130471

ACCGCGGCCGCGCGCCCCCCTCTGCCCCCGCTGCCCAGGATTGGTAGGCTCCACCGCTCGGCAGCCGGCTTCCCTGCTCGGACGCCGAGCACCGCCAAAG CGCACTTCGATTTTCAGAATTCCTCCTGGGAATGCTGACTCCTTGCTTGGTGCCCTGATGCTTCTCTGAGATAAACTGATGAATTGGAACCATGGTGCAA AAGAAGAAGTTCTGTCCTCGGTTACTTGACTATCTAGTGATCGTAGGGGCCAGGCACCCGAGCAGTGATAGCGTGGCCCAGACTCCTGAATTGCTACGGC GATACCCCTTGGAGGATCACACTGAGTTTCCCCTGCCCCCAGATGTAGTGTTCTTCTGCCAGCCCGAGGGCTGCCTGAGCGTGCGGCAGCGGCGCATGAG CCTTCGGGATGATACCTCTTTTGTCTTCACCCTCACTGACAAGGACACTGGAGTCACGCGATATGGCATCTGTGTTAACTTCTACCGCTCCTTCCAAAAG CGAATCTCTAAGGAGAAGGGGGAAGGTGGGGCAGGGTCCCGTGGGAAGGAAGGAACCCATGCCACCTGTGCCTCAGAAGAGGGTGGCACTGAGAGCTCAG AGAGTGGCTCATCCCTGCAGCCTCTCAGTGCTGACTCTACCCCTGATGTGAACCAGTCTCCTCGGGGCAAACGCCGGGCCAAGGCGGGGAGCCGCTCCCG CAACAGTACTCTCACGTCCCTGTGCGTGCTCAGCCACTACCCTTTCTTCTCCACCTTCCGAGAGTGTTTGTATACTCTCAAGCGCCTGGTGGACTGCTGT AGTGAGCGCCTTCTGGGCAAGAAACTGGGCATCCCTCGAGGCGTACAAAGGGACACCATGTGGCGGATCTTTACTGGATCGCTGCTGGTAGAGGAGAAGT CAAGTGCCCTTCTGCATGACCTTCGAGAGATTGAGGCCTGGATCTATCGATTGCTGCGCTCCCCAGTACCCGTCTCTGGGCAGAAGCGAGTAGACATCGA GGTCCTACCCCAAGAGCTCCAGCCAGCTCTGACCTTTGCTCTTCCAGACCCATCTCGATTCACCCTAGTGGATTTCCCACTGCACCTTCCCTTGGAACTT CTAGGTGTGGACGCCTGTCTCCAGGTGCTAACCTGCATTCTGTTAGAGCACAAGGTGGTGCTACAGTCCCGAGACTACAATGCACTCTCCATGTCTGTGA TGGCATTCGTGGCAATGATCTACCCACTGGAGTATATGTTTCCTGTCATCCCGCTGCTACCCACCTGCATGGCATCAGCAGAGCAGCTGCTGTTGGCTCC AACCCCGTACATCATTGGGGTTCCTGCCAGCTTCTTCCTCTACAAACTGGACTTCAAAATGCCTGATGATGTATGGCTAGTGGATCTGGACAGCAATAGG GTGATTGCCCCCACCAATGCAGAAGTGCTGCCTATCCTGCCAGAACCAGAATCACTAGAGCTGAAAAAGCATTTAAAGCAGGCCTTGGCCAGCATGAGTC TCAACACCCAGCCCATCCTCAATCTGGAGAAATTTCATGAGGGCCAGGAGATCCCCCTTCTCTTGGGAAGGCCTTCTAATGACCTGCAGTCCACACCGTC CACTGAATTCAACCCACTCATCTATGGCAATGATGTGGATTCTGTGGATGTTGCAACCAGGGTTGCCATGGTACGGTTCTTCAATTCCGCCAACGTGCTG CAGGGATTTCAGATGCACACGCGTACCCTGCGCCTCTTTCCTCGGCCTGTGGTAGCTTTTCAAGCTGGCTCCTTTCTAGCCTCACGTCCCCGGCAGACTC CTTTTGCCGAGAAATTGGCCAGGACTCAGGCTGTGGAGTACTTTGGGGAATGGATCCTTAACCCCACCAACTATGCCTTTCAGCGAATTCACAACAATAT GTTTGATCCAGCCCTGATTGGTGACAAGCCAAAGTGGTATGCTCATCAGCTGCAGCCTATCCACTATCGCGTCTATGACAGCAATTCCCAGCTGGCTGAG GCCCTGAGTGTACCACCAGAGCGGGACTCTGACTCCGAACCTACTGATGATAGTGGCAGTGATAGTATGGATTATGACGATTCAAGCTCTTCTTACTCCT CCCTTGGTGACTTTGTCAGTGAAATGATGAAATGTGACATTAATGGTGATACTCCCAATGTGGACCCTCTGACACATGCAGCACTGGGGGATGCCAGCGA GGTGGAGATTGACGAGCTGCAGAATCAGAAGGAAGCAGAAGAGCCTGGCCCAGACAGTGAGAACTCTCAGGAAAACCCCCCACTGCGCTCCAGCTCTAGC ACCACAGCCAGCAGCAGCCCCAGCACTGTCATCCACGGAGCCAACTCTGAACCTGCTGACTCTACGGAGATGGATGATAAGGCAGCAGTAGGCGTCTCCA AGCCCCTCCCTTCCGTGCCTCCCAGCATTGGCAAATCGAACGTGGACAGACGTCAGGCAGAAATTGGAGAGGGGGCTCAAAAGCTGCTGCGGCCCAACAG CTTGAGACTGGCAAGTGACTCAGATGCAGAGTCAGACTCTCGGGCAAGCTCTCCCAACTCCACCGTCTCCAACACCAGCACCGAGGGCTTCGGGGGCATC ATGTCTTTTGCCAGCAGCCTCTATCGGAACCACAGTACCAGCTTCAGTCTTTCAAACCTCACACTGCCCACCAAAGGTGCCCGAGAGAAGGCCACGCCCT TCCCCAGTCTGAAAGTATTTGGGCTAAATACTCTAATGGAGATTGTTACTGAAGCCGGCCCCGGGAGTGGTGAAGGAAACAGGAGGGCGTTAGTGGATCA GAAGTCATCTGTCATTAAACACAGCCCAACAGTGAAAAGAGAACCTCCATCACCCCAGGGTCGATCCAGCAATTCTAGTGAGAACCAGCAGTTCCTGAAG GAGGTGGTGCACAGCGTGCTGGACGGCCAGGGAGTTGGCTGGCTCAACATGAAAAAGGTGCGCCGGCTGCTGGAGAGCGAGCAGCTGCGAGTCTTTGTCC TGAGCAAGCTGAACCGCATGGTGCAGTCAGAGGACGATGCCCGGCAGGACATCATCCCGGATGTGGAGATCAGTCGGAAGGTGTACAAGGGAATGTTAGA CCTCCTCAAGTGTACAGTCCTCAGCTTGGAGCAGTCCTATGCCCACGCGGGTCTGGGTGGCATGGCCAGCATCTTTGGGCTTTTGGAGATTGCCCAGACC CACTACTATAGTAAAGAACCAGACAAGCGGAAGAGAAGTCCAACAGAAAGTGTAAATACCCCAGTTGGCAAGGATCCTGGCCTAGCTGGGCGGGGGGACC CAAAGGCTATGGCACAACTGAGAGTTCCACAACTGGGACCTCGGGCACCAAGTGCCACAGGAAAGGGTCCTAAGGAACTGGACACCAGAAGTTTAAAGGA AGAAAATTTTATAGCATCTATTGGGCCTGAAGTAATCAAACCTGTCTTTGACCTTGGTGAGACAGAGGAGAAAAAGTCCCAGATCAGCGCAGACAGTGGT GTGAGCCTGACGTCTAGTTCCCAGAGGACTGATCAAGACTCTGTCATCGGCGTGAGTCCAGCTGTTATGATCCGCAGCTCAAGTCAGGATTCTGAAGTTA GCACCGTGGTGAGTAATAGCTCTGGAGAGACCCTTGGAGCTGACAGTGACTTGAGCAGCAATGCAGGTGATGGACCAGGTGGCGAGGGCAGTGTTCACCT GGCAAGCTCTCGGGGCACTTTGTCTGATAGTGAAATTGAGACCAACTCTGCCACAAGCACCATCTTTGGTAAAGCCCACAGCTTGAAGCCAAGCATAAAG GAGAAGCTGGCAGGCAGCCCCATTCGTACTTCTGAAGATGTGAGCCAGCGAGTCTATCTCTATGAGGGACTCCTAGGCAAAGAGCGTTCTACTTTATGGG ACCAAATGCAATTCTGGGAAGATGCCTTCTTAGATGCTGTGATGTTGGAGAGAGAAGGGATGGGTATGGACCAGGGTCCCCAGGAAATGATCGACAGGTA CCTGTCCCTTGGAGAACATGACCGGAAGCGCCTGGAAGATGATGAAGATCGCTTGTTGGCCACACTTCTGCACAACCTCATCTCCTACATGCTGCTGATG AAGGTAAATAAGAATGACATCCGCAAGAAGGTGAGGCGCCTAATGGGAAAGTCGCACATTGGGCTTGTGTACAGCCAGCAAATCAATGAGGTGCTTGATC AGCTGGCGAACCTGAATGGACGCGATCTCTCTATCTGGTCCAGTGGCAGCCGGCACATGAAGAAGCAGACATTTGTGGTACATGCAGGGACAGATACAAA CGGAGATATCTTTTTCATGGAGGTGTGCGATGACTGTGTGGTGTTGCGTAGTAACATCGGAACAGTGTATGAGCGCTGGTGGTACGAGAAGCTCATCAAC ATGACCTACTGTCCCAAGACGAAGGTGTTGTGCTTGTGGCGTAGAAATGGCTCTGAGACCCAGCTCAACAAGTTCTATACTAAAAAGTGTCGGGAGCTGT ACTACTGTGTGAAGGACAGCATGGAGCGCGCTGCCGCCCGACAGCAAAGCATCAAACCCGGACCTGAATTGGGTGGCGAGTTCCCTGTGCAGGACCTGAA GACTGGTGAGGGTGGCCTGCTGCAGGTGACCCTGGAAGGGATCAACCTCAAATTCATGCACAATCAGGTTTTCATAGAGCTGAATCACATTAAAAAGTGC AATACAGTTCGAGGCGTCTTTGTCCTGGAGGAATTTGTTCCTGAAATTAAAGAAGTGGTGAGCCACAAGTACAAGACACCAATGGCCCACGAAATCTGCT ACTCCGTATTATGTCTCTTCTCGTACGTGGCTGCAGTTCATAGCAGTGAGGAAGATCTCAGAACCCCGCCCCGGCCTGTCTCTAGCTGATGGAGAGGGGC TACGCAGCTGCCCCAGCCCAGGGCACGCCCCTGGCCCCTTGCTGTTCCCAAGTGCACGATGCTGCTGTGACTGAGGAGTGGATGATGCTCGTGTGTCCTC TGCAAGCCCCCTGCTGTGGCTTGGTTGGTTACCGGTTATGTGTCCCTCTGAGTGTGTCTTGAGCGTGTCCACCTTCTCCCTCTCCACTCCCAGAAGACCA AACTGCCTTCCCCTCAGGGCTCAAGAATGTGTACAGTCTGTGGGGCCGGTGTGAACCCACTATTTTGTGTCCTTGAGACATTTGTGTTGTGGTTCCTTGT CCTTGTCCCTGGCGTTATAACTGTCCACTGCAAGAGTCTGGCTCTCCCTTCTCTGTGACCCGGCATGACTGGGCGCCTGGAGCAGTTTCACTCTGTGAGG AGTGAGGGAACCCTGGGGCTCACCCTCTCAGAGGAAGGGCACAGAGAGGAAGGGAAGAATTGGGGGGCAGCCGGAGTGAGTGGCAGCCTCCCTGCTTCCT TCTGCATTCCCAAGCCGGCAGCTACTGCCCAGGGCCCGCAGTGTTGGCTGCTGCCTGCCACAGCCTCTGTGACTGCAGTGGAGCGGCGAATTCCCTGTGG CCTGCCATGCCTTCGGCATCAGAGGATGGAGTGGTCGAGGCTAGTGGAGTCCCAGGGACCGCTGGCTGCTCTGCCTGAGCATCAGGGAGGGGGCAGGAAA GACCAAGCTGGGTTTGCACATCTGTCTGCAGGCTGTCTCTCCAGGCACGGGGTGTCAGGAGGGAGAGACAGCCTGGGTATGGGCAAGAAATGACTGTAAA TATTTCAGCCCCACATTATTTATAGAAAATGTACAGTTGTGTGAATGTGAAATAAATGTCCTCAACTCCC

refseq_MADD.F8 (forward primer)
refseq_MADD.R8 (reverse primer)
Single exon skipping

Frame 0

TAAARPPLPPLPRIGRLHRSAAGFPARTPSTAK AHFDFQNSSWEC*LLAWCPDASLR*TDELEPWCK RRSSVLGYLTI**S*GPGTRAVIAWPRLLNCYG DTPWRITLSFPCPQM*CSSASPRAA*ACGSGA* AFGMIPLLSSPSLTRTLESRDMASVLTSTAPSKS ESLRRRGKVGQGPVGRKEPMPPVPQKRVALRAQ RVAHPCSLSVLTLPLM*TSLLGANAGPRRGAAP ATVLSRPCACSATTLSSPPSESVCILSSAWWTAV VSAFWARNWASLEAYKGTPCGGSLLDRCW*RRS QVPFCMTFERLRPGSIDCCAPQYPSLGRSE*TS RSYPKSSSQL*PLLFQTHLDSP*WISHCTFPWNF *VWTPVSRC*PAFC*STRWCYSPETTMHSPCL* WHSWQ*STHWSICFLSSRCYPPAWHQQSSCCWL QPRTSLGFLPASSSTNWTSKCLMMYG*WIWTAIG *LPPPMQKCCLSCQNQNH*S*KSI*SRPWPA*V STPSPSSIWRNFMRARRSPFSWEGLLMTCSPHR PLNSTHSSMAMMWILWMLQPGLPWYGSSIPPTCC RDFRCTRVPCASFLGLW*LFKLAPF*PHVPGRL LLPRNWPGLRLWSTLGNGSLTPPTMPFSEFTTI CLIQP*LVTSQSGMLISCSLSTIASMTAIPSWLR P*VYHQSGTLTPNLLMIVAVIVWIMTIQALLTP PLVTLSVK**NVTLMVILPMWTL*HMQHWGMPA RWRLTSCRIRRKQKSLAQTVRTLRKTPHCAPALA PQPAAAPALSSTEPTLNLLTLRRWMIRQQ*ASP SPSLPCLPALANRTWTDVRQKLERGLKSCCGPT A*DWQVTQMQSQTLGQALPTPPSPTPAPRASGAS CLLPAASIGTTVPASVFQTSHCPPKVPERRPRP SPV*KYLG*IL*WRLLLKPAPGVVKETGGR*WI RSHLSLNTAQQ*KENLHHPRVDPAILVRTSSS*R RWCTACWTARELAGST*KRCAGCWRASSCESLS *AS*TAWCSQRTMPGRTSSRMWRSVGRCTREC* TSSSVQSSAWSSPMPTRVWVAWPASLGFWRLPRP TTIVKNQTSGREVQQKV*IPQLARILA*LGGGT QRLWHN*EFHNWDLGHQVPQERVLRNWTPEV*R KKIL*HLLGLK*SNLSLTLVRQRRKSPRSAQTVV *A*RLVPRGLIKTLSSA*VQLL*SAAQVRILKL APW*VIALERPLELTVT*AAMQVMDQVARAVFT WQALGALCLIVKLRPTLPQAPSLVKPTA*SQA*R RSWQAAPFVLLKM*ASESISMRDS*AKSVLLYG TKCNSGKMPS*ML*CWREKGWVWTRVPRK*STG TCPLENMTGSAWKMMKIACWPHFCTTSSPTCC** R*IRMTSARR*GA*WESRTLGLCTASKSMRCLI SWRT*MDAISLSGPVAAGT*RSRHLWYMQGQIQ TEISFSWRCAMTVWCCVVTSEQCMSAGGTRSSST *PTVPRRRCCACGVEMALRPSSTSSILKSVGSC TTV*RTAWSALPPDSKASNPDLNWVASSLCRT* RLVRVACCR*PWKGSTSNSCTIRFS*S*ITLKSA IQFEASLSWRNLFLKLKKW*ATSTRHQWPTKSA TPYYVSSRTWLQFIAVRKISEPRPGLSLADGEG LRSCPSPGHAPGPLLFPSARCCCD*GVDDARVSS ASPLLWLGWLPVMCPSECVLSVSTFSLSTPRRP NCLPLRAQECVQSVGPV*THYFVSLRHLCCGSL SLSLAL*LSTARVWLSLLCDPA*LGAWSSFTL*G VREPWGSPSQRKGTERKGRIGGQPE*VAASLLP SAFPSRQLLPRARSVGCCLPQPL*LQWSGEFPV ACHAFGIRGWSGRG*WSPRDRWLLCLSIREGAGK TKLGLHICLQAVSPGTGCQEGETAWVWARNDCK YFSPTLFIENVQLCECEINVLNS

Frame 1

PRPRAPLCPRCPGLVGSTARQPASLLGRRAPPK RTSIFRIPPGNADSLLGALMLL*DKLMNWNHGA KEEVLSSVT*LSSDRRGQAPEQ**RGPDS*IATA IPLGGSH*VSPAPRCSVLLPARGLPERAAAAHE PSG*YLFCLHPH*QGHWSHAIWHLC*LLPLLPK ANL*GEGGRWGRVPWEGRNPCHLCLRRGWH*ELR EWLIPAASQC*LYP*CEPVSSGQTPGQGGEPLP QQYSHVPVRAQPLPFLLHLPRVFVYSQAPGGLL **APSGQETGHPSRRTKGHHVADLYWIAAGRGEV KCPSA*PSRD*GLDLSIAALPSTRLWAEASRHR GPTPRAPASSDLCSSRPISIHPSGFPTAPSLGT SRCGRLSPGANLHSVRAQGGATVPRLQCTLHVCD GIRGNDLPTGVYVSCHPAATHLHGISRAAAVGS NPVHHWGSCQLLPLQTGLQNA**CMASGSGQQ* GDCPHQCRSAAYPARTRITRAEKAFKAGLGQHES QHPAHPQSGEIS*GPGDPPSLGKAF**PAVHTV H*IQPTHLWQ*CGFCGCCNQGCHGTVLQFRQRA AGISDAHAYPAPLSSACGSFSSWLLSSLTSPADS FCREIGQDSGCGVLWGMDP*PHQLCLSANSQQY V*SSPDW*QAKVVCSSAAAYPLSRL*QQFPAG* GPECTTRAGL*LRTY***WQ**YGL*RFKLFLLL PW*LCQ*NDEM*H*W*YSQCGPSDTCSTGGCQR GGD*RAAESEGSRRAWPRQ*ELSGKPPTALQL* HHSQQQPQHCHPRSQL*TC*LYGDG**GSSRRLQ APPFRASQHWQIERGQTSGRNWRGGSKAAAAQQ LETGK*LRCRVRLSGKLSQLHRLQHQHRGLRGH HVFCQQPLSEPQYQLQSFKPHTAHQRCPREGHAL PQSESIWAKYSNGDCY*SRPREW*RKQEGVSGS EVICH*TQPNSEKRTSITPGSIQQF**EPAVPE GGGAQRAGRPGSWLAQHEKGAPAAGERAAASLCP EQAEPHGAVRGRCPAGHHPGCGDQSEGVQGNVR PPQVYSPQLGAVLCPRGSGWHGQHLWAFGDCPD PLL**RTRQAEEKSNRKCKYPSWQGSWPSWAGGP KGYGTTESSTTGTSGTKCHRKGS*GTGHQKFKG RKFYSIYWA*SNQTCL*PW*DRGEKVPDQRRQW CEPDV*FPED*SRLCHRRESSCYDPQLKSGF*S* HRGE**LWRDPWS*Q*LEQQCR*WTRWRGQCSP GKLSGHFV***N*DQLCHKHHLW*SPQLEAKHK GEAGRQPHSYF*RCEPASLSL*GTPRQRAFYFMG PNAILGRCLLRCCDVGERRDGYGPGSPGNDRQV PVPWRT*PEAPGR**RSLVGHTSAQPHLLHAAD EGK*E*HPQEGEAPNGKVAHWACVQPANQ*GA*S AGEPEWTRSLYLVQWQPAHEEADICGTCRDRYK RRYLFHGGVR*LCGVA**HRNSV*ALVVREAHQ HDLLSQDEGVVLVA*KWL*DPAQQVLY*KVSGAV LLCEGQHGARCRPTAKHQTRT*IGWRVPCAGPE DW*GWPAAGDPGRDQPQIHAQSGFHRAESH*KV QYSSRRLCPGGICS*N*RSGEPQVQDTNGPRNLL LRIMSLLVRGCSS*Q*GRSQNPAPACL*LMERG YAAAPAQGTPLAPCCSQVHDAAVTEEWMMLVCP LQAPCCGLVGYRLCVPLSVS*ACPPSPSPLPEDQ TAFPSGLKNVYSLWGRCEPTILCP*DICVVVPC PCPWRYNCPLQESGSPFSVTRHDWAPGAVSLCE E*GNPGAHPLRGRAQRGREELGGSRSEWQPPCFL LHSQAGSYCPGPAVLAAACHSLCDCSGAANSLW PAMPSASEDGVVEASGVPGTAGCSA*ASGRGQE RPSWVCTSVCRLSLQARGVRRERQPGYGQEMTVN ISAPHYL*KMYSCVNVK*MSSTP

Frame 2

RGRAPPSAPAAQDW*APPLGSRLPCSDAEHRQS ALRFSEFLLGMLTPCLVP*CFSEIN**IGTMVQ KKKFCPRLLDYLVIVGARHPSSDSVAQTPELLR RYPLEDHTEFPLPPDVVFFCQPEGCLSVRQRRMS LRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQK RISKEKGEGGAGSRGKEGTHATCASEEGGTESS ESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSR NSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCC SERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEK SSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIE VLPQELQPALTFALPDPSRFTLVDFPLHLPLEL LGVDACLQVLTCILLEHKVVLQSRDYNALSMSV MAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAP TPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNR VIAPTNAEVLPILPEPESLELKKHLKQALASMS LNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPS TEFNPLIYGNDVDSVDVATRVAMVRFFNSANVL QGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQT PFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNM FDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAE ALSVPPERDSDSEPTDDSGSDSMDYDDSSSSYS SLGDFVSEMMKCDINGDTPNVDPLTHAALGDASE VEIDELQNQKEAEEPGPDSENSQENPPLRSSSS TTASSSPSTVIHGANSEPADSTEMDDKAAVGVS KPLPSVPPSIGKSNVDRRQAEIGEGAQKLLRPNS LRLASDSDAESDSRASSPNSTVSNTSTEGFGGI MSFASSLYRNHSTSFSLSNLTLPTKGAREKATP FPSLKVFGLNTLMEIVTEAGPGSGEGNRRALVDQ KSSVIKHSPTVKREPPSPQGRSSNSSENQQFLK EVVHSVLDGQGVGWLNMKKVRRLLESEQLRVFV LSKLNRMVQSEDDARQDIIPDVEISRKVYKGMLD LLKCTVLSLEQSYAHAGLGGMASIFGLLEIAQT HYYSKEPDKRKRSPTESVNTPVGKDPGLAGRGD PKAMAQLRVPQLGPRAPSATGKGPKELDTRSLKE ENFIASIGPEVIKPVFDLGETEEKKSQISADSG VSLTSSSQRTDQDSVIGVSPAVMIRSSSQDSEV STVVSNSSGETLGADSDLSSNAGDGPGGEGSVHL ASSRGTLSDSEIETNSATSTIFGKAHSLKPSIK EKLAGSPIRTSEDVSQRVYLYEGLLGKERSTLW DQMQFWEDAFLDAVMLEREGMGMDQGPQEMIDRY LSLGEHDRKRLEDDEDRLLATLLHNLISYMLLM KVNKNDIRKKVRRLMGKSHIGLVYSQQINEVLD QLANLNGRDLSIWSSGSRHMKKQTFVVHAGTDTN GDIFFMEVCDDCVVLRSNIGTVYERWWYEKLIN MTYCPKTKVLCLWRRNGSETQLNKFYTKKCREL YYCVKDSMERAAARQQSIKPGPELGGEFPVQDLK TGEGGLLQVTLEGINLKFMHNQVFIELNHIKKC NTVRGVFVLEEFVPEIKEVVSHKYKTPMAHEIC YSVLCLFSYVAAVHSSEEDLRTPPRPVSS*WRGA TQLPQPRARPWPLAVPKCTMLL*LRSG*CSCVL CKPPAVAWLVTGYVSL*VCLERVHLLPLHSQKT KLPSPQGSRMCTVCGAGVNPLFCVLETFVLWFLV LVPGVITVHCKSLALPSL*PGMTGRLEQFHSVR SEGTLGLTLSEEGHREEGKNWGAAGVSGSLPAS FCIPKPAATAQGPQCWLLPATASVTAVERRIPCG LPCLRHQRMEWSRLVESQGPLAALPEHQGGGRK DQAGFAHLSAGCLSRHGVSGGRDSLGMGKK*L* IFQPHIIYRKCTVV*M*NKCPQL

Sequence NM_130475

AGTGCAGACGCGGAACTGGCGCCCGCTCGGAGCGGCGCCGCGCTGGGGAGCGACTGACGCCCCGCTGCCGGGGGACGTCGGGCTGGGCCTGGCCAGTCCC CCAGAGCTTGGGAGACTTCGATTTTCAGAATTCCTCCTGGGAATGCTGACTCCTTGCTTGGTGCCCTGATGCTTCTCTGAGATAAACTGATGAATTGGAA CCATGGTGCAAAAGAAGAAGTTCTGTCCTCGGTTACTTGACTATCTAGTGATCGTAGGGGCCAGGCACCCGAGCAGTGATAGCGTGGCCCAGACTCCTGA ATTGCTACGGCGATACCCCTTGGAGGATCACACTGAGTTTCCCCTGCCCCCAGATGTAGTGTTCTTCTGCCAGCCCGAGGGCTGCCTGAGCGTGCGGCAG CGGCGCATGAGCCTTCGGGATGATACCTCTTTTGTCTTCACCCTCACTGACAAGGACACTGGAGTCACGCGATATGGCATCTGTGTTAACTTCTACCGCT CCTTCCAAAAGCGAATCTCTAAGGAGAAGGGGGAAGGTGGGGCAGGGTCCCGTGGGAAGGAAGGAACCCATGCCACCTGTGCCTCAGAAGAGGGTGGCAC TGAGAGCTCAGAGAGTGGCTCATCCCTGCAGCCTCTCAGTGCTGACTCTACCCCTGATGTGAACCAGTCTCCTCGGGGCAAACGCCGGGCCAAGGCGGGG AGCCGCTCCCGCAACAGTACTCTCACGTCCCTGTGCGTGCTCAGCCACTACCCTTTCTTCTCCACCTTCCGAGAGTGTTTGTATACTCTCAAGCGCCTGG TGGACTGCTGTAGTGAGCGCCTTCTGGGCAAGAAACTGGGCATCCCTCGAGGCGTACAAAGGGACACCATGTGGCGGATCTTTACTGGATCGCTGCTGGT AGAGGAGAAGTCAAGTGCCCTTCTGCATGACCTTCGAGAGATTGAGGCCTGGATCTATCGATTGCTGCGCTCCCCAGTACCCGTCTCTGGGCAGAAGCGA GTAGACATCGAGGTCCTACCCCAAGAGCTCCAGCCAGCTCTGACCTTTGCTCTTCCAGACCCATCTCGATTCACCCTAGTGGATTTCCCACTGCACCTTC CCTTGGAACTTCTAGGTGTGGACGCCTGTCTCCAGGTGCTAACCTGCATTCTGTTAGAGCACAAGGTGGTGCTACAGTCCCGAGACTACAATGCACTCTC CATGTCTGTGATGGCATTCGTGGCAATGATCTACCCACTGGAGTATATGTTTCCTGTCATCCCGCTGCTACCCACCTGCATGGCATCAGCAGAGCAGCTG CTGTTGGCTCCAACCCCGTACATCATTGGGGTTCCTGCCAGCTTCTTCCTCTACAAACTGGACTTCAAAATGCCTGATGATGTATGGCTAGTGGATCTGG ACAGCAATAGGGTGATTGCCCCCACCAATGCAGAAGTGCTGCCTATCCTGCCAGAACCAGAATCACTAGAGCTGAAAAAGCATTTAAAGCAGGCCTTGGC CAGCATGAGTCTCAACACCCAGCCCATCCTCAATCTGGAGAAATTTCATGAGGGCCAGGAGATCCCCCTTCTCTTGGGAAGGCCTTCTAATGACCTGCAG TCCACACCGTCCACTGAATTCAACCCACTCATCTATGGCAATGATGTGGATTCTGTGGATGTTGCAACCAGGGTTGCCATGGTACGGTTCTTCAATTCCG CCAACGTGCTGCAGGGATTTCAGATGCACACGCGTACCCTGCGCCTCTTTCCTCGGCCTGTGGTAGCTTTTCAAGCTGGCTCCTTTCTAGCCTCACGTCC CCGGCAGACTCCTTTTGCCGAGAAATTGGCCAGGACTCAGGCTGTGGAGTACTTTGGGGAATGGATCCTTAACCCCACCAACTATGCCTTTCAGCGAATT CACAACAATATGTTTGATCCAGCCCTGATTGGTGACAAGCCAAAGTGGTATGCTCATCAGCTGCAGCCTATCCACTATCGCGTCTATGACAGCAATTCCC AGCTGGCTGAGGCCCTGAGTGTACCACCAGAGCGGGACTCTGACTCCGAACCTACTGATGATAGTGGCAGTGATAGTATGGATTATGACGATTCAAGCTC TTCTTACTCCTCCCTTGGTGACTTTGTCAGTGAAATGATGAAATGTGACATTAATGGTGATACTCCCAATGTGGACCCTCTGACACATGCAGCACTGGGG GATGCCAGCGAGGTGGAGATTGACGAGCTGCAGAATCAGAAGGAAGCAGAAGAGCCTGGCCCAGACAGTGAGAACTCTCAGGAAAACCCCCCACTGCGCT CCAGCTCTAGCACCACAGCCAGCAGCAGCCCCAGCACTGTCATCCACGGAGCCAACTCTGAACCTGCTGACTCTACGGAGATGGATGATAAGGCAGCAGT AGGCGTCTCCAAGCCCCTCCCTTCCGTGCCTCCCAGCATTGGCAAATCGAACGTGGACAGACGTCAGGCAGAAATTGGAGAGGGGTCAGTGCGCCGGCGA ATCTATGACAATCCATACTTCGAGCCCCAATATGGCTTTCCCCCTGAGGAAGATGAGGATGAGCAGGGGGAAAGTTACACTCCCCGATTCAGCCAACATG TCAGTGGCAATCGGGCTCAAAAGCTGCTGCGGCCCAACAGCTTGAGACTGGCAAGTGACTCAGATGCAGAGTCAGACTCTCGGGCAAGCTCTCCCAACTC CACCGTCTCCAACACCAGCACCGAGGGCTTCGGGGGCATCATGTCTTTTGCCAGCAGCCTCTATCGGAACCACAGTACCAGCTTCAGTCTTTCAAACCTC ACACTGCCCACCAAAGGTGCCCGAGAGAAGGCCACGCCCTTCCCCAGTCTGAAAGTATTTGGGCTAAATACTCTAATGGAGATTGTTACTGAAGCCGGCC CCGGGAGTGGTGAAGGAAACAGGAGGGCGTTAGTGGATCAGAAGTCATCTGTCATTAAACACAGCCCAACAGTGAAAAGAGAACCTCCATCACCCCAGGG TCGATCCAGCAATTCTAGTGAGAACCAGCAGTTCCTGAAGGAGGTGGTGCACAGCGTGCTGGACGGCCAGGGAGTTGGCTGGCTCAACATGAAAAAGGTG CGCCGGCTGCTGGAGAGCGAGCAGCTGCGAGTCTTTGTCCTGAGCAAGCTGAACCGCATGGTGCAGTCAGAGGACGATGCCCGGCAGGACATCATCCCGG ATGTGGAGATCAGTCGGAAGGTGTACAAGGGAATGTTAGACCTCCTCAAGTGTACAGTCCTCAGCTTGGAGCAGTCCTATGCCCACGCGGGTCTGGGTGG CATGGCCAGCATCTTTGGGCTTTTGGAGATTGCCCAGACCCACTACTATAGTAAAGAACCAGACAAGCGGAAGAGAAGTCCAACAGAAAGTGTAAATACC CCAGTTGGCAAGGATCCTGGCCTAGCTGGGCGGGGGGACCCAAAGGCTATGGCACAACTGAGAGTTCCACAACTGGGACCTCGGGCACCAAGTGCCACAG GAAAGGGTCCTAAGGAACTGGACACCAGAAGTTTAAAGGAAGAAAATTTTATAGCATCTATTGAATTGTGGAACAAGCACCAGGAAGTGAAAAAGCAAAA AGCTTTGGAAAAACAGAGGCCTGAAGTAATCAAACCTGTCTTTGACCTTGGTGAGACAGAGGAGAAAAAGTCCCAGATCAGCGCAGACAGTGGTGTGAGC CTGACGTCTAGTTCCCAGAGGACTGATCAAGACTCTGTCATCGGCGTGAGTCCAGCTGTTATGATCCGCAGCTCAAGTCAGGATTCTGAAGTTAGCACCG TGGTGAGTAATAGCTCTGGAGAGACCCTTGGAGCTGACAGTGACTTGAGCAGCAATGCAGGTGATGGACCAGGTGGCGAGGGCAGTGTTCACCTGGCAAG CTCTCGGGGCACTTTGTCTGATAGTGAAATTGAGACCAACTCTGCCACAAGCACCATCTTTGGTAAAGCCCACAGCTTGAAGCCAAGCATAAAGGAGAAG CTGGCAGGCAGCCCCATTCGTACTTCTGAAGATGTGAGCCAGCGAGTCTATCTCTATGAGGGACTCCTAGGAAGGGACAAAGGATCCATGTGGGACCAGT TAGAGGATGCAGCTATGGAGACCTTTTCTATAAGCAAAGAGCGTTCTACTTTATGGGACCAAATGCAATTCTGGGAAGATGCCTTCTTAGATGCTGTGAT GTTGGAGAGAGAAGGGATGGGTATGGACCAGGGTCCCCAGGAAATGATCGACAGGTACCTGTCCCTTGGAGAACATGACCGGAAGCGCCTGGAAGATGAT GAAGATCGCTTGTTGGCCACACTTCTGCACAACCTCATCTCCTACATGCTGCTGATGAAGGTAAATAAGAATGACATCCGCAAGAAGGTGAGGCGCCTAA TGGGAAAGTCGCACATTGGGCTTGTGTACAGCCAGCAAATCAATGAGGTGCTTGATCAGCTGGCGAACCTGAATGGACGCGATCTCTCTATCTGGTCCAG TGGCAGCCGGCACATGAAGAAGCAGACATTTGTGGTACATGCAGGGACAGATACAAACGGAGATATCTTTTTCATGGAGGTGTGCGATGACTGTGTGGTG TTGCGTAGTAACATCGGAACAGTGTATGAGCGCTGGTGGTACGAGAAGCTCATCAACATGACCTACTGTCCCAAGACGAAGGTGTTGTGCTTGTGGCGTA GAAATGGCTCTGAGACCCAGCTCAACAAGTTCTATACTAAAAAGTGTCGGGAGCTGTACTACTGTGTGAAGGACAGCATGGAGCGCGCTGCCGCCCGACA GCAAAGCATCAAACCCGGACCTGAATTGGGTGGCGAGTTCCCTGTGCAGGACCTGAAGACTGGTGAGGGTGGCCTGCTGCAGGTGACCCTGGAAGGGATC AACCTCAAATTCATGCACAATCAGTTCCTGAAATTAAAGAAGTGGTGAGCCACAAGTACAAGACACCAATGGCCCACGAAATCTGCTACTCCGTATTATG TCTCTTCTCGTACGTGGCTGCAGTTCATAGCAGTGAGGAAGATCTCAGAACCCCGCCCCGGCCTGTCTCTAGCTGATGGAGAGGGGCTACGCAGCTGCCC CAGCCCAGGGCACGCCCCTGGCCCCTTGCTGTTCCCAAGTGCACGATGCTGCTGTGACTGAGGAGTGGATGATGCTCGTGTGTCCTCTGCAAGCCCCCTG CTGTGGCTTGGTTGGTTACCGGTTATGTGTCCCTCTGAGTGTGTCTTGAGCGTGTCCACCTTCTCCCTCTCCACTCCCAGAAGACCAAACTGCCTTCCCC TCAGGGCTCAAGAATGTGTACAGTCTGTGGGGCCGGTGTGAACCCACTATTTTGTGTCCTTGAGACATTTGTGTTGTGGTTCCTTGTCCTTGTCCCTGGC GTTATAACTGTCCACTGCAAGAGTCTGGCTCTCCCTTCTCTGTGACCCGGCATGACTGGGCGCCTGGAGCAGTTTCACTCTGTGAGGAGTGAGGGAACCC TGGGGCTCACCCTCTCAGAGGAAGGGCACAGAGAGGAAGGGAAGAATTGGGGGGCAGCCGGAGTGAGTGGCAGCCTCCCTGCTTCCTTCTGCATTCCCAA GCCGGCAGCTACTGCCCAGGGCCCGCAGTGTTGGCTGCTGCCTGCCACAGCCTCTGTGACTGCAGTGGAGCGGCGAATTCCCTGTGGCCTGCCATGCCTT CGGCATCAGAGGATGGAGTGGTCGAGGCTAGTGGAGTCCCAGGGACCGCTGGCTGCTCTGCCTGAGCATCAGGGAGGGGGCAGGAAAGACCAAGCTGGGT TTGCACATCTGTCTGCAGGCTGTCTCTCCAGGCACGGGGTGTCAGGAGGGAGAGACAGCCTGGGTATGGGCAAGAAATGACTGTAAATATTTCAGCCCCA CATTATTTATAGAAAATGTACAGTTGTGTGAATGTGAAATAAATGTCCTCAACTCCC

refseq_MADD.F8 (forward primer)
refseq_MADD.R8 (reverse primer)
Single exon skipping

Frame 0

SADAELAPARSGAALGSD*RPAAGGRRAGPGQS PRAWETSIFRIPPGNADSLLGALMLL*DKLMNWN HGAKEEVLSSVT*LSSDRRGQAPEQ**RGPDS* IATAIPLGGSH*VSPAPRCSVLLPARGLPERAA AAHEPSG*YLFCLHPH*QGHWSHAIWHLC*LLPL LPKANL*GEGGRWGRVPWEGRNPCHLCLRRGWH *ELREWLIPAASQC*LYP*CEPVSSGQTPGQGG EPLPQQYSHVPVRAQPLPFLLHLPRVFVYSQAPG GLL**APSGQETGHPSRRTKGHHVADLYWIAAG RGEVKCPSA*PSRD*GLDLSIAALPSTRLWAEA SRHRGPTPRAPASSDLCSSRPISIHPSGFPTAPS LGTSRCGRLSPGANLHSVRAQGGATVPRLQCTL HVCDGIRGNDLPTGVYVSCHPAATHLHGISRAA AVGSNPVHHWGSCQLLPLQTGLQNA**CMASGSG QQ*GDCPHQCRSAAYPARTRITRAEKAFKAGLG QHESQHPAHPQSGEIS*GPGDPPSLGKAF**PA VHTVH*IQPTHLWQ*CGFCGCCNQGCHGTVLQFR QRAAGISDAHAYPAPLSSACGSFSSWLLSSLTS PADSFCREIGQDSGCGVLWGMDP*PHQLCLSAN SQQYV*SSPDW*QAKVVCSSAAAYPLSRL*QQFP AG*GPECTTRAGL*LRTY***WQ**YGL*RFKL FLLLPW*LCQ*NDEM*H*W*YSQCGPSDTCSTG GCQRGGD*RAAESEGSRRAWPRQ*ELSGKPPTAL QL*HHSQQQPQHCHPRSQL*TC*LYGDG**GSS RRLQAPPFRASQHWQIERGQTSGRNWRGVSAPA NL*QSILRAPIWLSP*GR*G*AGGKLHSPIQPTC QWQSGSKAAAAQQLETGK*LRCRVRLSGKLSQL HRLQHQHRGLRGHHVFCQQPLSEPQYQLQSFKP HTAHQRCPREGHALPQSESIWAKYSNGDCY*SRP REW*RKQEGVSGSEVICH*TQPNSEKRTSITPG SIQQF**EPAVPEGGGAQRAGRPGSWLAQHEKG APAAGERAAASLCPEQAEPHGAVRGRCPAGHHPG CGDQSEGVQGNVRPPQVYSPQLGAVLCPRGSGW HGQHLWAFGDCPDPLL**RTRQAEEKSNRKCKY PSWQGSWPSWAGGPKGYGTTESSTTGTSGTKCHR KGS*GTGHQKFKGRKFYSIY*IVEQAPGSEKAK SFGKTEA*SNQTCL*PW*DRGEKVPDQRRQWCE PDV*FPED*SRLCHRRESSCYDPQLKSGF*S*HR GE**LWRDPWS*Q*LEQQCR*WTRWRGQCSPGK LSGHFV***N*DQLCHKHHLW*SPQLEAKHKGE AGRQPHSYF*RCEPASLSL*GTPRKGQRIHVGPV RGCSYGDLFYKQRAFYFMGPNAILGRCLLRCCD VGERRDGYGPGSPGNDRQVPVPWRT*PEAPGR* *RSLVGHTSAQPHLLHAADEGK*E*HPQEGEAPN GKVAHWACVQPANQ*GA*SAGEPEWTRSLYLVQ WQPAHEEADICGTCRDRYKRRYLFHGGVR*LCG VA**HRNSV*ALVVREAHQHDLLSQDEGVVLVA* KWL*DPAQQVLY*KVSGAVLLCEGQHGARCRPT AKHQTRT*IGWRVPCAGPEDW*GWPAAGDPGRD QPQIHAQSVPEIKEVVSHKYKTPMAHEICYSVLC LFSYVAAVHSSEEDLRTPPRPVSS*WRGATQLP QPRARPWPLAVPKCTMLL*LRSG*CSCVLCKPP AVAWLVTGYVSL*VCLERVHLLPLHSQKTKLPSP QGSRMCTVCGAGVNPLFCVLETFVLWFLVLVPG VITVHCKSLALPSL*PGMTGRLEQFHSVRSEGT LGLTLSEEGHREEGKNWGAAGVSGSLPASFCIPK PAATAQGPQCWLLPATASVTAVERRIPCGLPCL RHQRMEWSRLVESQGPLAALPEHQGGGRKDQAG FAHLSAGCLSRHGVSGGRDSLGMGKK*L*IFQPH IIYRKCTVV*M*NKCPQL

Frame 1

VQTRNWRPLGAAPRWGATDAPLPGDVGLGLASP PELGRLRFSEFLLGMLTPCLVP*CFSEIN**IG TMVQKKKFCPRLLDYLVIVGARHPSSDSVAQTPE LLRRYPLEDHTEFPLPPDVVFFCQPEGCLSVRQ RRMSLRDDTSFVFTLTDKDTGVTRYGICVNFYR SFQKRISKEKGEGGAGSRGKEGTHATCASEEGGT ESSESGSSLQPLSADSTPDVNQSPRGKRRAKAG SRSRNSTLTSLCVLSHYPFFSTFRECLYTLKRL VDCCSERLLGKKLGIPRGVQRDTMWRIFTGSLLV EEKSSALLHDLREIEAWIYRLLRSPVPVSGQKR VDIEVLPQELQPALTFALPDPSRFTLVDFPLHL PLELLGVDACLQVLTCILLEHKVVLQSRDYNALS MSVMAFVAMIYPLEYMFPVIPLLPTCMASAEQL LLAPTPYIIGVPASFFLYKLDFKMPDDVWLVDL DSNRVIAPTNAEVLPILPEPESLELKKHLKQALA SMSLNTQPILNLEKFHEGQEIPLLLGRPSNDLQ STPSTEFNPLIYGNDVDSVDVATRVAMVRFFNS ANVLQGFQMHTRTLRLFPRPVVAFQAGSFLASRP RQTPFAEKLARTQAVEYFGEWILNPTNYAFQRI HNNMFDPALIGDKPKWYAHQLQPIHYRVYDSNS QLAEALSVPPERDSDSEPTDDSGSDSMDYDDSSS SYSSLGDFVSEMMKCDINGDTPNVDPLTHAALG DASEVEIDELQNQKEAEEPGPDSENSQENPPLR SSSSTTASSSPSTVIHGANSEPADSTEMDDKAAV GVSKPLPSVPPSIGKSNVDRRQAEIGEGSVRRR IYDNPYFEPQYGFPPEEDEDEQGESYTPRFSQH VSGNRAQKLLRPNSLRLASDSDAESDSRASSPNS TVSNTSTEGFGGIMSFASSLYRNHSTSFSLSNL TLPTKGAREKATPFPSLKVFGLNTLMEIVTEAG PGSGEGNRRALVDQKSSVIKHSPTVKREPPSPQG RSSNSSENQQFLKEVVHSVLDGQGVGWLNMKKV RRLLESEQLRVFVLSKLNRMVQSEDDARQDIIP DVEISRKVYKGMLDLLKCTVLSLEQSYAHAGLGG MASIFGLLEIAQTHYYSKEPDKRKRSPTESVNT PVGKDPGLAGRGDPKAMAQLRVPQLGPRAPSAT GKGPKELDTRSLKEENFIASIELWNKHQEVKKQK ALEKQRPEVIKPVFDLGETEEKKSQISADSGVS LTSSSQRTDQDSVIGVSPAVMIRSSSQDSEVST VVSNSSGETLGADSDLSSNAGDGPGGEGSVHLAS SRGTLSDSEIETNSATSTIFGKAHSLKPSIKEK LAGSPIRTSEDVSQRVYLYEGLLGRDKGSMWDQ LEDAAMETFSISKERSTLWDQMQFWEDAFLDAVM LEREGMGMDQGPQEMIDRYLSLGEHDRKRLEDD EDRLLATLLHNLISYMLLMKVNKNDIRKKVRRL MGKSHIGLVYSQQINEVLDQLANLNGRDLSIWSS GSRHMKKQTFVVHAGTDTNGDIFFMEVCDDCVV LRSNIGTVYERWWYEKLINMTYCPKTKVLCLWR RNGSETQLNKFYTKKCRELYYCVKDSMERAAARQ QSIKPGPELGGEFPVQDLKTGEGGLLQVTLEGI NLKFMHNQFLKLKKW*ATSTRHQWPTKSATPYY VSSRTWLQFIAVRKISEPRPGLSLADGEGLRSCP SPGHAPGPLLFPSARCCCD*GVDDARVSSASPL LWLGWLPVMCPSECVLSVSTFSLSTPRRPNCLP LRAQECVQSVGPV*THYFVSLRHLCCGSLSLSLA L*LSTARVWLSLLCDPA*LGAWSSFTL*GVREP WGSPSQRKGTERKGRIGGQPE*VAASLLPSAFP SRQLLPRARSVGCCLPQPL*LQWSGEFPVACHAF GIRGWSGRG*WSPRDRWLLCLSIREGAGKTKLG LHICLQAVSPGTGCQEGETAWVWARNDCKYFSP TLFIENVQLCECEINVLNS

Frame 2

CRRGTGARSERRRAGERLTPRCRGTSGWAWPVP QSLGDFDFQNSSWEC*LLAWCPDASLR*TDELE PWCKRRSSVLGYLTI**S*GPGTRAVIAWPRLL NCYGDTPWRITLSFPCPQM*CSSASPRAA*ACGS GA*AFGMIPLLSSPSLTRTLESRDMASVLTSTA PSKSESLRRRGKVGQGPVGRKEPMPPVPQKRVA LRAQRVAHPCSLSVLTLPLM*TSLLGANAGPRRG AAPATVLSRPCACSATTLSSPPSESVCILSSAW WTAVVSAFWARNWASLEAYKGTPCGGSLLDRCW *RRSQVPFCMTFERLRPGSIDCCAPQYPSLGRSE *TSRSYPKSSSQL*PLLFQTHLDSP*WISHCTF PWNF*VWTPVSRC*PAFC*STRWCYSPETTMHS PCL*WHSWQ*STHWSICFLSSRCYPPAWHQQSSC CWLQPRTSLGFLPASSSTNWTSKCLMMYG*WIW TAIG*LPPPMQKCCLSCQNQNH*S*KSI*SRPW PA*VSTPSPSSIWRNFMRARRSPFSWEGLLMTCS PHRPLNSTHSSMAMMWILWMLQPGLPWYGSSIP PTCCRDFRCTRVPCASFLGLW*LFKLAPF*PHV PGRLLLPRNWPGLRLWSTLGNGSLTPPTMPFSEF TTICLIQP*LVTSQSGMLISCSLSTIASMTAIP SWLRP*VYHQSGTLTPNLLMIVAVIVWIMTIQA LLTPPLVTLSVK**NVTLMVILPMWTL*HMQHWG MPARWRLTSCRIRRKQKSLAQTVRTLRKTPHCA PALAPQPAAAPALSSTEPTLNLLTLRRWMIRQQ *ASPSPSLPCLPALANRTWTDVRQKLERGQCAGE SMTIHTSSPNMAFPLRKMRMSRGKVTLPDSANM SVAIGLKSCCGPTA*DWQVTQMQSQTLGQALPT PPSPTPAPRASGASCLLPAASIGTTVPASVFQTS HCPPKVPERRPRPSPV*KYLG*IL*WRLLLKPA PGVVKETGGR*WIRSHLSLNTAQQ*KENLHHPR VDPAILVRTSSS*RRWCTACWTARELAGST*KRC AGCWRASSCESLS*AS*TAWCSQRTMPGRTSSR MWRSVGRCTREC*TSSSVQSSAWSSPMPTRVWV AWPASLGFWRLPRPTTIVKNQTSGREVQQKV*IP QLARILA*LGGGTQRLWHN*EFHNWDLGHQVPQ ERVLRNWTPEV*RKKIL*HLLNCGTSTRK*KSK KLWKNRGLK*SNLSLTLVRQRRKSPRSAQTVV*A *RLVPRGLIKTLSSA*VQLL*SAAQVRILKLAP W*VIALERPLELTVT*AAMQVMDQVARAVFTWQ ALGALCLIVKLRPTLPQAPSLVKPTA*SQA*RRS WQAAPFVLLKM*ASESISMRDS*EGTKDPCGTS *RMQLWRPFL*AKSVLLYGTKCNSGKMPS*ML* CWREKGWVWTRVPRK*STGTCPLENMTGSAWKMM KIACWPHFCTTSSPTCC**R*IRMTSARR*GA* WESRTLGLCTASKSMRCLISWRT*MDAISLSGP VAAGT*RSRHLWYMQGQIQTEISFSWRCAMTVWC CVVTSEQCMSAGGTRSSST*PTVPRRRCCACGV EMALRPSSTSSILKSVGSCTTV*RTAWSALPPD SKASNPDLNWVASSLCRT*RLVRVACCR*PWKGS TSNSCTISS*N*RSGEPQVQDTNGPRNLLLRIM SLLVRGCSS*Q*GRSQNPAPACL*LMERGYAAA PAQGTPLAPCCSQVHDAAVTEEWMMLVCPLQAPC CGLVGYRLCVPLSVS*ACPPSPSPLPEDQTAFP SGLKNVYSLWGRCEPTILCP*DICVVVPCPCPW RYNCPLQESGSPFSVTRHDWAPGAVSLCEE*GNP GAHPLRGRAQRGREELGGSRSEWQPPCFLLHSQ AGSYCPGPAVLAAACHSLCDCSGAANSLWPAMP SASEDGVVEASGVPGTAGCSA*ASGRGQERPSWV CTSVCRLSLQARGVRRERQPGYGQEMTVNISAP HYL*KMYSCVNVK*MSSTP

Sequence NM_003682

ACCGCGGCCGCGCGCCCCCCTCTGCCCCCGCTGCCCAGGATTGGTAGGCTCCACCGCTCGGCAGCCGGCTTCCCTGCTCGGACGCCGAGCACCGCCAAAG CGCACTTCGATTTTCAGAATTCCTCCTGGGAATGCTGACTCCTTGCTTGGTGCCCTGATGCTTCTCTGAGATAAACTGATGAATTGGAACCATGGTGCAA AAGAAGAAGTTCTGTCCTCGGTTACTTGACTATCTAGTGATCGTAGGGGCCAGGCACCCGAGCAGTGATAGCGTGGCCCAGACTCCTGAATTGCTACGGC GATACCCCTTGGAGGATCACACTGAGTTTCCCCTGCCCCCAGATGTAGTGTTCTTCTGCCAGCCCGAGGGCTGCCTGAGCGTGCGGCAGCGGCGCATGAG CCTTCGGGATGATACCTCTTTTGTCTTCACCCTCACTGACAAGGACACTGGAGTCACGCGATATGGCATCTGTGTTAACTTCTACCGCTCCTTCCAAAAG CGAATCTCTAAGGAGAAGGGGGAAGGTGGGGCAGGGTCCCGTGGGAAGGAAGGAACCCATGCCACCTGTGCCTCAGAAGAGGGTGGCACTGAGAGCTCAG AGAGTGGCTCATCCCTGCAGCCTCTCAGTGCTGACTCTACCCCTGATGTGAACCAGTCTCCTCGGGGCAAACGCCGGGCCAAGGCGGGGAGCCGCTCCCG CAACAGTACTCTCACGTCCCTGTGCGTGCTCAGCCACTACCCTTTCTTCTCCACCTTCCGAGAGTGTTTGTATACTCTCAAGCGCCTGGTGGACTGCTGT AGTGAGCGCCTTCTGGGCAAGAAACTGGGCATCCCTCGAGGCGTACAAAGGGACACCATGTGGCGGATCTTTACTGGATCGCTGCTGGTAGAGGAGAAGT CAAGTGCCCTTCTGCATGACCTTCGAGAGATTGAGGCCTGGATCTATCGATTGCTGCGCTCCCCAGTACCCGTCTCTGGGCAGAAGCGAGTAGACATCGA GGTCCTACCCCAAGAGCTCCAGCCAGCTCTGACCTTTGCTCTTCCAGACCCATCTCGATTCACCCTAGTGGATTTCCCACTGCACCTTCCCTTGGAACTT CTAGGTGTGGACGCCTGTCTCCAGGTGCTAACCTGCATTCTGTTAGAGCACAAGGTGGTGCTACAGTCCCGAGACTACAATGCACTCTCCATGTCTGTGA TGGCATTCGTGGCAATGATCTACCCACTGGAGTATATGTTTCCTGTCATCCCGCTGCTACCCACCTGCATGGCATCAGCAGAGCAGCTGCTGTTGGCTCC AACCCCGTACATCATTGGGGTTCCTGCCAGCTTCTTCCTCTACAAACTGGACTTCAAAATGCCTGATGATGTATGGCTAGTGGATCTGGACAGCAATAGG GTGATTGCCCCCACCAATGCAGAAGTGCTGCCTATCCTGCCAGAACCAGAATCACTAGAGCTGAAAAAGCATTTAAAGCAGGCCTTGGCCAGCATGAGTC TCAACACCCAGCCCATCCTCAATCTGGAGAAATTTCATGAGGGCCAGGAGATCCCCCTTCTCTTGGGAAGGCCTTCTAATGACCTGCAGTCCACACCGTC CACTGAATTCAACCCACTCATCTATGGCAATGATGTGGATTCTGTGGATGTTGCAACCAGGGTTGCCATGGTACGGTTCTTCAATTCCGCCAACGTGCTG CAGGGATTTCAGATGCACACGCGTACCCTGCGCCTCTTTCCTCGGCCTGTGGTAGCTTTTCAAGCTGGCTCCTTTCTAGCCTCACGTCCCCGGCAGACTC CTTTTGCCGAGAAATTGGCCAGGACTCAGGCTGTGGAGTACTTTGGGGAATGGATCCTTAACCCCACCAACTATGCCTTTCAGCGAATTCACAACAATAT GTTTGATCCAGCCCTGATTGGTGACAAGCCAAAGTGGTATGCTCATCAGCTGCAGCCTATCCACTATCGCGTCTATGACAGCAATTCCCAGCTGGCTGAG GCCCTGAGTGTACCACCAGAGCGGGACTCTGACTCCGAACCTACTGATGATAGTGGCAGTGATAGTATGGATTATGACGATTCAAGCTCTTCTTACTCCT CCCTTGGTGACTTTGTCAGTGAAATGATGAAATGTGACATTAATGGTGATACTCCCAATGTGGACCCTCTGACACATGCAGCACTGGGGGATGCCAGCGA GGTGGAGATTGACGAGCTGCAGAATCAGAAGGAAGCAGAAGAGCCTGGCCCAGACAGTGAGAACTCTCAGGAAAACCCCCCACTGCGCTCCAGCTCTAGC ACCACAGCCAGCAGCAGCCCCAGCACTGTCATCCACGGAGCCAACTCTGAACCTGCTGACTCTACGGAGATGGATGATAAGGCAGCAGTAGGCGTCTCCA AGCCCCTCCCTTCCGTGCCTCCCAGCATTGGCAAATCGAACGTGGACAGACGTCAGGCAGAAATTGGAGAGGGGTCAGTGCGCCGGCGAATCTATGACAA TCCATACTTCGAGCCCCAATATGGCTTTCCCCCTGAGGAAGATGAGGATGAGCAGGGGGAAAGTTACACTCCCCGATTCAGCCAACATGTCAGTGGCAAT CGGGCTCAAAAGCTGCTGCGGCCCAACAGCTTGAGACTGGCAAGTGACTCAGATGCAGAGTCAGACTCTCGGGCAAGCTCTCCCAACTCCACCGTCTCCA ACACCAGCACCGAGGGCTTCGGGGGCATCATGTCTTTTGCCAGCAGCCTCTATCGGAACCACAGTACCAGCTTCAGTCTTTCAAACCTCACACTGCCCAC CAAAGGTGCCCGAGAGAAGGCCACGCCCTTCCCCAGTCTGAAAGTATTTGGGCTAAATACTCTAATGGAGATTGTTACTGAAGCCGGCCCCGGGAGTGGT GAAGGAAACAGGAGGGCGTTAGTGGATCAGAAGTCATCTGTCATTAAACACAGCCCAACAGTGAAAAGAGAACCTCCATCACCCCAGGGTCGATCCAGCA ATTCTAGTGAGAACCAGCAGTTCCTGAAGGAGGTGGTGCACAGCGTGCTGGACGGCCAGGGAGTTGGCTGGCTCAACATGAAAAAGGTGCGCCGGCTGCT GGAGAGCGAGCAGCTGCGAGTCTTTGTCCTGAGCAAGCTGAACCGCATGGTGCAGTCAGAGGACGATGCCCGGCAGGACATCATCCCGGATGTGGAGATC AGTCGGAAGGTGTACAAGGGAATGTTAGACCTCCTCAAGTGTACAGTCCTCAGCTTGGAGCAGTCCTATGCCCACGCGGGTCTGGGTGGCATGGCCAGCA TCTTTGGGCTTTTGGAGATTGCCCAGACCCACTACTATAGTAAAGAACCAGACAAGCGGAAGAGAAGTCCAACAGAAAGTGTAAATACCCCAGTTGGCAA GGATCCTGGCCTAGCTGGGCGGGGGGACCCAAAGGCTATGGCACAACTGAGAGTTCCACAACTGGGACCTCGGGCACCAAGTGCCACAGGAAAGGGTCCT AAGGAACTGGACACCAGAAGTTTAAAGGAAGAAAATTTTATAGCATCTATTGAATTGTGGAACAAGCACCAGGAAGTGAAAAAGCAAAAAGCTTTGGAAA AACAGAGGCCTGAAGTAATCAAACCTGTCTTTGACCTTGGTGAGACAGAGGAGAAAAAGTCCCAGATCAGCGCAGACAGTGGTGTGAGCCTGACGTCTAG TTCCCAGAGGACTGATCAAGACTCTGTCATCGGCGTGAGTCCAGCTGTTATGATCCGCAGCTCAAGTCAGGATTCTGAAGTTAGCACCGTGGTGAGTAAT AGCTCTGGAGAGACCCTTGGAGCTGACAGTGACTTGAGCAGCAATGCAGGTGATGGACCAGGTGGCGAGGGCAGTGTTCACCTGGCAAGCTCTCGGGGCA CTTTGTCTGATAGTGAAATTGAGACCAACTCTGCCACAAGCACCATCTTTGGTAAAGCCCACAGCTTGAAGCCAAGCATAAAGGAGAAGCTGGCAGGCAG CCCCATTCGTACTTCTGAAGATGTGAGCCAGCGAGTCTATCTCTATGAGGGACTCCTAGGAAGGGACAAAGGATCCATGTGGGACCAGTTAGAGGATGCA GCTATGGAGACCTTTTCTATAAGCAAAGAGCGTTCTACTTTATGGGACCAAATGCAATTCTGGGAAGATGCCTTCTTAGATGCTGTGATGTTGGAGAGAG AAGGGATGGGTATGGACCAGGGTCCCCAGGAAATGATCGACAGGTACCTGTCCCTTGGAGAACATGACCGGAAGCGCCTGGAAGATGATGAAGATCGCTT GTTGGCCACACTTCTGCACAACCTCATCTCCTACATGCTGCTGATGAAGGTAAATAAGAATGACATCCGCAAGAAGGTGAGGCGCCTAATGGGAAAGTCG CACATTGGGCTTGTGTACAGCCAGCAAATCAATGAGGTGCTTGATCAGCTGGCGAACCTGAATGGACGCGATCTCTCTATCTGGTCCAGTGGCAGCCGGC ACATGAAGAAGCAGACATTTGTGGTACATGCAGGGACAGATACAAACGGAGATATCTTTTTCATGGAGGTGTGCGATGACTGTGTGGTGTTGCGTAGTAA CATCGGAACAGTGTATGAGCGCTGGTGGTACGAGAAGCTCATCAACATGACCTACTGTCCCAAGACGAAGGTGTTGTGCTTGTGGCGTAGAAATGGCTCT GAGACCCAGCTCAACAAGTTCTATACTAAAAAGTGTCGGGAGCTGTACTACTGTGTGAAGGACAGCATGGAGCGCGCTGCCGCCCGACAGCAAAGCATCA AACCCGGACCTGAATTGGGTGGCGAGTTCCCTGTGCAGGACCTGAAGACTGGTGAGGGTGGCCTGCTGCAGGTGACCCTGGAAGGGATCAACCTCAAATT CATGCACAATCAGGTTTTCATAGAGCTGAATCACATTAAAAAGTGCAATACAGTTCGAGGCGTCTTTGTCCTGGAGGAATTTGTTCCTGAAATTAAAGAA GTGGTGAGCCACAAGTACAAGACACCAATGGCCCACGAAATCTGCTACTCCGTATTATGTCTCTTCTCGTACGTGGCTGCAGTTCATAGCAGTGAGGAAG ATCTCAGAACCCCGCCCCGGCCTGTCTCTAGCTGATGGAGAGGGGCTACGCAGCTGCCCCAGCCCAGGGCACGCCCCTGGCCCCTTGCTGTTCCCAAGTG CACGATGCTGCTGTGACTGAGGAGTGGATGATGCTCGTGTGTCCTCTGCAAGCCCCCTGCTGTGGCTTGGTTGGTTACCGGTTATGTGTCCCTCTGAGTG TGTCTTGAGCGTGTCCACCTTCTCCCTCTCCACTCCCAGAAGACCAAACTGCCTTCCCCTCAGGGCTCAAGAATGTGTACAGTCTGTGGGGCCGGTGTGA ACCCACTATTTTGTGTCCTTGAGACATTTGTGTTGTGGTTCCTTGTCCTTGTCCCTGGCGTTATAACTGTCCACTGCAAGAGTCTGGCTCTCCCTTCTCT GTGACCCGGCATGACTGGGCGCCTGGAGCAGTTTCACTCTGTGAGGAGTGAGGGAACCCTGGGGCTCACCCTCTCAGAGGAAGGGCACAGAGAGGAAGGG AAGAATTGGGGGGCAGCCGGAGTGAGTGGCAGCCTCCCTGCTTCCTTCTGCATTCCCAAGCCGGCAGCTACTGCCCAGGGCCCGCAGTGTTGGCTGCTGC CTGCCACAGCCTCTGTGACTGCAGTGGAGCGGCGAATTCCCTGTGGCCTGCCATGCCTTCGGCATCAGAGGATGGAGTGGTCGAGGCTAGTGGAGTCCCA GGGACCGCTGGCTGCTCTGCCTGAGCATCAGGGAGGGGGCAGGAAAGACCAAGCTGGGTTTGCACATCTGTCTGCAGGCTGTCTCTCCAGGCACGGGGTG TCAGGAGGGAGAGACAGCCTGGGTATGGGCAAGAAATGACTGTAAATATTTCAGCCCCACATTATTTATAGAAAATGTACAGTTGTGTGAATGTGAAATA AATGTCCTCAACTCCC

refseq_MADD.F8 (forward primer)
refseq_MADD.R8 (reverse primer)
Single exon skipping

Frame 0

TAAARPPLPPLPRIGRLHRSAAGFPARTPSTAK AHFDFQNSSWEC*LLAWCPDASLR*TDELEPWCK RRSSVLGYLTI**S*GPGTRAVIAWPRLLNCYG DTPWRITLSFPCPQM*CSSASPRAA*ACGSGA* AFGMIPLLSSPSLTRTLESRDMASVLTSTAPSKS ESLRRRGKVGQGPVGRKEPMPPVPQKRVALRAQ RVAHPCSLSVLTLPLM*TSLLGANAGPRRGAAP ATVLSRPCACSATTLSSPPSESVCILSSAWWTAV VSAFWARNWASLEAYKGTPCGGSLLDRCW*RRS QVPFCMTFERLRPGSIDCCAPQYPSLGRSE*TS RSYPKSSSQL*PLLFQTHLDSP*WISHCTFPWNF *VWTPVSRC*PAFC*STRWCYSPETTMHSPCL* WHSWQ*STHWSICFLSSRCYPPAWHQQSSCCWL QPRTSLGFLPASSSTNWTSKCLMMYG*WIWTAIG *LPPPMQKCCLSCQNQNH*S*KSI*SRPWPA*V STPSPSSIWRNFMRARRSPFSWEGLLMTCSPHR PLNSTHSSMAMMWILWMLQPGLPWYGSSIPPTCC RDFRCTRVPCASFLGLW*LFKLAPF*PHVPGRL LLPRNWPGLRLWSTLGNGSLTPPTMPFSEFTTI CLIQP*LVTSQSGMLISCSLSTIASMTAIPSWLR P*VYHQSGTLTPNLLMIVAVIVWIMTIQALLTP PLVTLSVK**NVTLMVILPMWTL*HMQHWGMPA RWRLTSCRIRRKQKSLAQTVRTLRKTPHCAPALA PQPAAAPALSSTEPTLNLLTLRRWMIRQQ*ASP SPSLPCLPALANRTWTDVRQKLERGQCAGESMT IHTSSPNMAFPLRKMRMSRGKVTLPDSANMSVAI GLKSCCGPTA*DWQVTQMQSQTLGQALPTPPSP TPAPRASGASCLLPAASIGTTVPASVFQTSHCP PKVPERRPRPSPV*KYLG*IL*WRLLLKPAPGVV KETGGR*WIRSHLSLNTAQQ*KENLHHPRVDPA ILVRTSSS*RRWCTACWTARELAGST*KRCAGC WRASSCESLS*AS*TAWCSQRTMPGRTSSRMWRS VGRCTREC*TSSSVQSSAWSSPMPTRVWVAWPA SLGFWRLPRPTTIVKNQTSGREVQQKV*IPQLA RILA*LGGGTQRLWHN*EFHNWDLGHQVPQERVL RNWTPEV*RKKIL*HLLNCGTSTRK*KSKKLWK NRGLK*SNLSLTLVRQRRKSPRSAQTVV*A*RL VPRGLIKTLSSA*VQLL*SAAQVRILKLAPW*VI ALERPLELTVT*AAMQVMDQVARAVFTWQALGA LCLIVKLRPTLPQAPSLVKPTA*SQA*RRSWQA APFVLLKM*ASESISMRDS*EGTKDPCGTS*RMQ LWRPFL*AKSVLLYGTKCNSGKMPS*ML*CWRE KGWVWTRVPRK*STGTCPLENMTGSAWKMMKIA CWPHFCTTSSPTCC**R*IRMTSARR*GA*WESR TLGLCTASKSMRCLISWRT*MDAISLSGPVAAG T*RSRHLWYMQGQIQTEISFSWRCAMTVWCCVV TSEQCMSAGGTRSSST*PTVPRRRCCACGVEMAL RPSSTSSILKSVGSCTTV*RTAWSALPPDSKAS NPDLNWVASSLCRT*RLVRVACCR*PWKGSTSN SCTIRFS*S*ITLKSAIQFEASLSWRNLFLKLKK W*ATSTRHQWPTKSATPYYVSSRTWLQFIAVRK ISEPRPGLSLADGEGLRSCPSPGHAPGPLLFPS ARCCCD*GVDDARVSSASPLLWLGWLPVMCPSEC VLSVSTFSLSTPRRPNCLPLRAQECVQSVGPV* THYFVSLRHLCCGSLSLSLAL*LSTARVWLSLL CDPA*LGAWSSFTL*GVREPWGSPSQRKGTERKG RIGGQPE*VAASLLPSAFPSRQLLPRARSVGCC LPQPL*LQWSGEFPVACHAFGIRGWSGRG*WSP RDRWLLCLSIREGAGKTKLGLHICLQAVSPGTGC QEGETAWVWARNDCKYFSPTLFIENVQLCECEI NVLNS

Frame 1

PRPRAPLCPRCPGLVGSTARQPASLLGRRAPPK RTSIFRIPPGNADSLLGALMLL*DKLMNWNHGA KEEVLSSVT*LSSDRRGQAPEQ**RGPDS*IATA IPLGGSH*VSPAPRCSVLLPARGLPERAAAAHE PSG*YLFCLHPH*QGHWSHAIWHLC*LLPLLPK ANL*GEGGRWGRVPWEGRNPCHLCLRRGWH*ELR EWLIPAASQC*LYP*CEPVSSGQTPGQGGEPLP QQYSHVPVRAQPLPFLLHLPRVFVYSQAPGGLL **APSGQETGHPSRRTKGHHVADLYWIAAGRGEV KCPSA*PSRD*GLDLSIAALPSTRLWAEASRHR GPTPRAPASSDLCSSRPISIHPSGFPTAPSLGT SRCGRLSPGANLHSVRAQGGATVPRLQCTLHVCD GIRGNDLPTGVYVSCHPAATHLHGISRAAAVGS NPVHHWGSCQLLPLQTGLQNA**CMASGSGQQ* GDCPHQCRSAAYPARTRITRAEKAFKAGLGQHES QHPAHPQSGEIS*GPGDPPSLGKAF**PAVHTV H*IQPTHLWQ*CGFCGCCNQGCHGTVLQFRQRA AGISDAHAYPAPLSSACGSFSSWLLSSLTSPADS FCREIGQDSGCGVLWGMDP*PHQLCLSANSQQY V*SSPDW*QAKVVCSSAAAYPLSRL*QQFPAG* GPECTTRAGL*LRTY***WQ**YGL*RFKLFLLL PW*LCQ*NDEM*H*W*YSQCGPSDTCSTGGCQR GGD*RAAESEGSRRAWPRQ*ELSGKPPTALQL* HHSQQQPQHCHPRSQL*TC*LYGDG**GSSRRLQ APPFRASQHWQIERGQTSGRNWRGVSAPANL*Q SILRAPIWLSP*GR*G*AGGKLHSPIQPTCQWQ SGSKAAAAQQLETGK*LRCRVRLSGKLSQLHRLQ HQHRGLRGHHVFCQQPLSEPQYQLQSFKPHTAH QRCPREGHALPQSESIWAKYSNGDCY*SRPREW *RKQEGVSGSEVICH*TQPNSEKRTSITPGSIQQ F**EPAVPEGGGAQRAGRPGSWLAQHEKGAPAA GERAAASLCPEQAEPHGAVRGRCPAGHHPGCGD QSEGVQGNVRPPQVYSPQLGAVLCPRGSGWHGQH LWAFGDCPDPLL**RTRQAEEKSNRKCKYPSWQ GSWPSWAGGPKGYGTTESSTTGTSGTKCHRKGS *GTGHQKFKGRKFYSIY*IVEQAPGSEKAKSFGK TEA*SNQTCL*PW*DRGEKVPDQRRQWCEPDV* FPED*SRLCHRRESSCYDPQLKSGF*S*HRGE* *LWRDPWS*Q*LEQQCR*WTRWRGQCSPGKLSGH FV***N*DQLCHKHHLW*SPQLEAKHKGEAGRQ PHSYF*RCEPASLSL*GTPRKGQRIHVGPVRGC SYGDLFYKQRAFYFMGPNAILGRCLLRCCDVGER RDGYGPGSPGNDRQVPVPWRT*PEAPGR**RSL VGHTSAQPHLLHAADEGK*E*HPQEGEAPNGKV AHWACVQPANQ*GA*SAGEPEWTRSLYLVQWQPA HEEADICGTCRDRYKRRYLFHGGVR*LCGVA** HRNSV*ALVVREAHQHDLLSQDEGVVLVA*KWL *DPAQQVLY*KVSGAVLLCEGQHGARCRPTAKHQ TRT*IGWRVPCAGPEDW*GWPAAGDPGRDQPQI HAQSGFHRAESH*KVQYSSRRLCPGGICS*N*R SGEPQVQDTNGPRNLLLRIMSLLVRGCSS*Q*GR SQNPAPACL*LMERGYAAAPAQGTPLAPCCSQV HDAAVTEEWMMLVCPLQAPCCGLVGYRLCVPLS VS*ACPPSPSPLPEDQTAFPSGLKNVYSLWGRCE PTILCP*DICVVVPCPCPWRYNCPLQESGSPFS VTRHDWAPGAVSLCEE*GNPGAHPLRGRAQRGR EELGGSRSEWQPPCFLLHSQAGSYCPGPAVLAAA CHSLCDCSGAANSLWPAMPSASEDGVVEASGVP GTAGCSA*ASGRGQERPSWVCTSVCRLSLQARG VRRERQPGYGQEMTVNISAPHYL*KMYSCVNVK* MSSTP

Frame 2

RGRAPPSAPAAQDW*APPLGSRLPCSDAEHRQS ALRFSEFLLGMLTPCLVP*CFSEIN**IGTMVQ KKKFCPRLLDYLVIVGARHPSSDSVAQTPELLR RYPLEDHTEFPLPPDVVFFCQPEGCLSVRQRRMS LRDDTSFVFTLTDKDTGVTRYGICVNFYRSFQK RISKEKGEGGAGSRGKEGTHATCASEEGGTESS ESGSSLQPLSADSTPDVNQSPRGKRRAKAGSRSR NSTLTSLCVLSHYPFFSTFRECLYTLKRLVDCC SERLLGKKLGIPRGVQRDTMWRIFTGSLLVEEK SSALLHDLREIEAWIYRLLRSPVPVSGQKRVDIE VLPQELQPALTFALPDPSRFTLVDFPLHLPLEL LGVDACLQVLTCILLEHKVVLQSRDYNALSMSV MAFVAMIYPLEYMFPVIPLLPTCMASAEQLLLAP TPYIIGVPASFFLYKLDFKMPDDVWLVDLDSNR VIAPTNAEVLPILPEPESLELKKHLKQALASMS LNTQPILNLEKFHEGQEIPLLLGRPSNDLQSTPS TEFNPLIYGNDVDSVDVATRVAMVRFFNSANVL QGFQMHTRTLRLFPRPVVAFQAGSFLASRPRQT PFAEKLARTQAVEYFGEWILNPTNYAFQRIHNNM FDPALIGDKPKWYAHQLQPIHYRVYDSNSQLAE ALSVPPERDSDSEPTDDSGSDSMDYDDSSSSYS SLGDFVSEMMKCDINGDTPNVDPLTHAALGDASE VEIDELQNQKEAEEPGPDSENSQENPPLRSSSS TTASSSPSTVIHGANSEPADSTEMDDKAAVGVS KPLPSVPPSIGKSNVDRRQAEIGEGSVRRRIYDN PYFEPQYGFPPEEDEDEQGESYTPRFSQHVSGN RAQKLLRPNSLRLASDSDAESDSRASSPNSTVS NTSTEGFGGIMSFASSLYRNHSTSFSLSNLTLPT KGAREKATPFPSLKVFGLNTLMEIVTEAGPGSG EGNRRALVDQKSSVIKHSPTVKREPPSPQGRSS NSSENQQFLKEVVHSVLDGQGVGWLNMKKVRRLL ESEQLRVFVLSKLNRMVQSEDDARQDIIPDVEI SRKVYKGMLDLLKCTVLSLEQSYAHAGLGGMAS IFGLLEIAQTHYYSKEPDKRKRSPTESVNTPVGK DPGLAGRGDPKAMAQLRVPQLGPRAPSATGKGP KELDTRSLKEENFIASIELWNKHQEVKKQKALE KQRPEVIKPVFDLGETEEKKSQISADSGVSLTSS SQRTDQDSVIGVSPAVMIRSSSQDSEVSTVVSN SSGETLGADSDLSSNAGDGPGGEGSVHLASSRG TLSDSEIETNSATSTIFGKAHSLKPSIKEKLAGS PIRTSEDVSQRVYLYEGLLGRDKGSMWDQLEDA AMETFSISKERSTLWDQMQFWEDAFLDAVMLER EGMGMDQGPQEMIDRYLSLGEHDRKRLEDDEDRL LATLLHNLISYMLLMKVNKNDIRKKVRRLMGKS HIGLVYSQQINEVLDQLANLNGRDLSIWSSGSR HMKKQTFVVHAGTDTNGDIFFMEVCDDCVVLRSN IGTVYERWWYEKLINMTYCPKTKVLCLWRRNGS ETQLNKFYTKKCRELYYCVKDSMERAAARQQSI KPGPELGGEFPVQDLKTGEGGLLQVTLEGINLKF MHNQVFIELNHIKKCNTVRGVFVLEEFVPEIKE VVSHKYKTPMAHEICYSVLCLFSYVAAVHSSEE DLRTPPRPVSS*WRGATQLPQPRARPWPLAVPKC TMLL*LRSG*CSCVLCKPPAVAWLVTGYVSL*V CLERVHLLPLHSQKTKLPSPQGSRMCTVCGAGV NPLFCVLETFVLWFLVLVPGVITVHCKSLALPSL *PGMTGRLEQFHSVRSEGTLGLTLSEEGHREEG KNWGAAGVSGSLPASFCIPKPAATAQGPQCWLL PATASVTAVERRIPCGLPCLRHQRMEWSRLVESQ GPLAALPEHQGGGRKDQAGFAHLSAGCLSRHGV SGGRDSLGMGKK*L*IFQPHIIYRKCTVV*M*N KCPQL