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Sequence NM_205839
AGGCTGGGGGAGAAGTTAAAGCCAGAGGAGGGGCAGGAATGTCTGAGGTGGCAACACTTCTCTTCAGCCAGACAGCACTGGCCAGTTTGGAGTCTGTCCATCCTGCAGGCCACAAGCTCTGGATGAGGAACTTGAGGCAAGTCACCAGCCCCTGATCATTTCGCCTAAAAGAGCAAGGACTAGAGTTCCTGACCTCCAGG
CCAGTCCCTGATCCCTGACCTAATGTTATCGCGGAATGATGGCCCAGGGCTCCTCAGAGCAGGAACTCCACTATGCATCTCTGCAGAGGCTGCCAGTGCC
CAGCAGTGAGGGACCTGACCTCAGGGGCAGAGACAAGAGAGGCACCAAGGAGGATCCAAGAGCTGACTATGCCTGCATTGCTGAGAACAAACCCACCTGA
GCACCCCAGACACCTTCCTCAACCCAGGCGGGTGGACAGGGTCCCCCTGTGGTCCAGCCAGTAAAAACCATGGTCCCCCCACTTCTGTGTCTCAGTCCTC
TCAGTCCATCTCGAGCCTCCGTTCAAATTGATCATCATCAAAACTTATGTGGCTTTTTGACCTTTGAATAGGGAATTTTTTAAATTTTTTAAAAATTAAA
ATAAAAAAAACACATGGCTCACCCTTCCACCCA
- refseq_LST1.F2 (forward primer)
- refseq_LST1.R2 (reverse primer)
- Multiple exon skipping
Frame 0
RLGEKLKPEEGQECLRWQHFSSARQHWPVWSLSILQATSSG*GT*GKSPAPDHFA*KSKD*SS*PPG
QSLIPDLMLSRNDGPGLLRAGTPLCISAEAASA
QQ*GT*PQGQRQERHQGGSKS*LCLHC*EQTHL
STPDTFLNPGGWTGSPCGPASKNHGPPTSVSQSS
QSISSLRSN*SSSKLMWLFDL*IGNFLNFLKIK
IKKTHGSPFHP
Frame 1
GWGRS*SQRRGRNV*GGNTSLQPDSTGQFGVCPSCRPQALDEELEASHQPLIISPKRARTRVPDLQ
ASP*SLT*CYRGMMAQGSSEQELHYASLQRLPVP
SSEGPDLRGRDKRGTKEDPRADYACIAENKPT*
APQTPSSTQAGGQGPPVVQPVKTMVPPLLCLSP
LSPSRASVQIDHHQNLCGFLTFE*GIF*IF*KLK
*KKHMAHPST
Frame 2
AGGEVKARGGAGMSEVATLLFSQTALASLESVHPAGHKLWMRNLRQVTSP*SFRLKEQGLEFLTSR
PVPDP*PNVIAE*WPRAPQSRNSTMHLCRGCQC
PAVRDLTSGAETREAPRRIQELTMPALLRTNPPE
HPRHLPQPRRVDRVPLWSSQ*KPWSPHFCVSVL
SVHLEPPFKLIIIKTYVAF*PLNREFFKFFKN*
NKKNTWLTLPP
Sequence NM_205837
CACCCACCCAGGCCCAGGCTCCTTCCCCTCCATCATCCCCTTACCAGCACCTAGAACCATCCAGGGCTGAAAAGTCCCCTCCAAACCACGTGATGAGGAACTTGAGGCAAGTCACCAGCCCCTGATCATTTCGCCTAAAAGAGCAAGGACTAGAGTTCCTGACCTCCAGGCCAGTCCCTGATCCCTGACCTAATGTTATC
GCGGAATGATGGCCCAGGGCTCCTCAGAGCAGGAACTCCACTATGCATCTCTGCAGAGGCTGCCAGTGCCCAGCAGTGAGGGACCTGACCTCAGGGGCAG
AGACAAGAGAGGCACCAAGGAGGATCCAAGAGCTGACTATGCCTGCATTGCTGAGAACAAACCCACCTGAGCACCCCAGACACCTTCCTCAACCCAGGCG
GGTGGACAGGGTCCCCCTGTGGTCCAGCCAGTAAAAACCATGGTCCCCCCACTTCTGTGTCTCAGTCCTCTCAGTCCATCTCGAGCCTCCGTTCAAATTG
ATCATCATCAAAACTTATGTGGCTTTTTGACCTTTGAATAGGGAATTTTTTAAATTTTTTAAAAATTAAAATAAAAAAAACACATGGCTCACCCTTCCAC
CCA
- refseq_LST1.F2 (forward primer)
- refseq_LST1.R2 (reverse primer)
- Multiple exon skipping
Frame 0
HPPRPRLLPLHHPLTST*NHPGLKSPLQTT**GT*GKSPAPDHFA*KSKD*SS*PPGQSLIPDLMLS
RNDGPGLLRAGTPLCISAEAASAQQ*GT*PQGQ
RQERHQGGSKS*LCLHC*EQTHLSTPDTFLNPG
GWTGSPCGPASKNHGPPTSVSQSSQSISSLRSN*
SSSKLMWLFDL*IGNFLNFLKIKIKKTHGSPFH
P
Frame 1
THPGPGSFPSIIPLPAPRTIQG*KVPSKPRDEELEASHQPLIISPKRARTRVPDLQASP*SLT*CY
RGMMAQGSSEQELHYASLQRLPVPSSEGPDLRGR
DKRGTKEDPRADYACIAENKPT*APQTPSSTQA
GGQGPPVVQPVKTMVPPLLCLSPLSPSRASVQI
DHHQNLCGFLTFE*GIF*IF*KLK*KKHMAHPST
Frame 2
PTQAQAPSPPSSPYQHLEPSRAEKSPPNHVMRNLRQVTSP*SFRLKEQGLEFLTSRPVPDP*PNVI
AE*WPRAPQSRNSTMHLCRGCQCPAVRDLTSGA
ETREAPRRIQELTMPALLRTNPPEHPRHLPQPRR
VDRVPLWSSQ*KPWSPHFCVSVLSVHLEPPFKL
IIIKTYVAF*PLNREFFKFFKN*NKKNTWLTLP
P
Sequence NM_007161
AGGCTGGGGGAGAAGTTAAAGCCAGAGGAGGGGCAGGAATGTCTGAGGTGGCAACACTTCTCTTCAGCCAGACAGCACTGGCCAGTTTGGAGTCTGTCCATCCTGCAGGCCACAAGCTCTGGATGAGGAACTTGAGGCAAGTCACCAGCCCCTGATCATTTCGCCTAAAAGAGCAAGGACTAGAGTTCCTGACCTCCAGG
CCAGTCCCTGATCCCTGACCTAATGTTATCGCGGAATGATGATATATGTATCTACGGGGGCCTGGGGCTGGGCGGGCTCCTGCTTCTGGCAGTGGTCCTT
CTGTCCGCCTGCCTGTGTTGGCTGCATCGAAGAGTAAAGAGGCTGGAGAGGAGCTGGGCCCAGGGCTCCTCAGAGCAGGAACTCCACTATGCATCTCTGC
AGAGGCTGCCAGTGCCCAGCAGTGAGGGACCTGACCTCAGGGGCAGAGACAAGAGAGGCACCAAGGAGGATCCAAGAGCTGACTATGCCTGCATTGCTGA
GAACAAACCCACCTGAGCACCCCAGACACCTTCCTCAACCCAGGCGGGTGGACAGGGTCCCCCTGTGGTCCAGCCAGTAAAAACCATGGTCCCCCCACTT
CTGTGTCTCAGTCCTCTCAGTCCATCTCGAGCCTCCGTTCAAATTGATCATCATCAAAACTTATGTGGCTTTTTGACCTTTGAATAGGGAATTTTTTAAA
TTTTTTAAAAATTAAAATAAAAAAAACACATGGCTCACCCTTCCACCCA
- refseq_LST1.F2 (forward primer)
- refseq_LST1.R2 (reverse primer)
- Multiple exon skipping
Frame 0
RLGEKLKPEEGQECLRWQHFSSARQHWPVWSLSILQATSSG*GT*GKSPAPDHFA*KSKD*SS*PPG
QSLIPDLMLSRNDDICIYGGLGLGGLLLLAVVL
LSACLCWLHRRVKRLERSWAQGSSEQELHYASL
QRLPVPSSEGPDLRGRDKRGTKEDPRADYACIAE
NKPT*APQTPSSTQAGGQGPPVVQPVKTMVPPL
LCLSPLSPSRASVQIDHHQNLCGFLTFE*GIF*
IF*KLK*KKHMAHPST
Frame 1
GWGRS*SQRRGRNV*GGNTSLQPDSTGQFGVCPSCRPQALDEELEASHQPLIISPKRARTRVPDLQ
ASP*SLT*CYRGMMIYVSTGAWGWAGSCFWQWSF
CPPACVGCIEE*RGWRGAGPRAPQSRNSTMHLC
RGCQCPAVRDLTSGAETREAPRRIQELTMPALL
RTNPPEHPRHLPQPRRVDRVPLWSSQ*KPWSPHF
CVSVLSVHLEPPFKLIIIKTYVAF*PLNREFFK
FFKN*NKKNTWLTLPP
Frame 2
AGGEVKARGGAGMSEVATLLFSQTALASLESVHPAGHKLWMRNLRQVTSP*SFRLKEQGLEFLTSR
PVPDP*PNVIAE**YMYLRGPGAGRAPASGSGP
SVRLPVLAASKSKEAGEELGPGLLRAGTPLCISA
EAASAQQ*GT*PQGQRQERHQGGSKS*LCLHC*
EQTHLSTPDTFLNPGGWTGSPCGPASKNHGPPT
SVSQSSQSISSLRSN*SSSKLMWLFDL*IGNFLN
FLKIKIKKTHGSPFHP
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