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Sequences

Sequence NM_004795

CGCGCAGCATGCCCGCCAGCGCCCCGCCGCGCCGCCCGCGGCCGCCGCCGCCGTCGCTGTCGCTGCTGCTGGTGCTGCTGGGCCTGGGCGGCCGCCGCCT
GCGTGCGGAGCCGGGCGACGGCGCGCAGACCTGGGCCCGTTTCTCGCGGCCTCCTGCCCCCGAGGCCGCGGGCCTCTTCCAGGGCACCTTCCCCGACGGC
TTCCTCTGGGCCGTGGGCAGCGCCGCCTACCAGACCGAGGGCGGCTGGCAGCAGCACGGCAAGGGTGCGTCCATCTGGGATACGTTCACCCACCACCCCC
TGGCACCCCCGGGAGACTCCCGGAACGCCAGTCTGCCGTTGGGCGCCCCGTCGCCGCTGCAGCCCGCCACCGGGGACGTAGCCAGCGACAGCTACAACAA
CGTCTTCCGCGACACGGAGGCGCTGCGCGAGCTCGGGGTCACTCACTACCGCTTCTCCATCTCGTGGGCGCGAGTGCTCCCCAATGGCAGCGCGGGCGTC
CCCAACCGCGAGGGGCTGCGCTACTACCGGCGCCTGCTGGAGCGGCTGCGGGAGCTGGGCGTGCAGCCCGTGGTCACCCTGTACCACTGGGACCTGCCCC
AGCGCCTGCAGGACGCCTACGGCGGCTGGGCCAACCGCGCCCTGGCCGACCACTTCAGGGATTACGCGGAGCTCTGCTTCCGCCACTTCGGCGGTCAGGT
CAAGTACTGGATCACCATCGACAACCCCTACGTGGTGGCCTGGCACGGCTACGCCACCGGGCGCCTGGCCCCCGGCATCCGGGGCAGCCCGCGGCTCGGG
TACCTGGTGGCGCACAACCTCCTCCTGGCTCATGCCAAAGTCTGGCATCTCTACAATACTTCTTTCCGTCCCACTCAGGGAGGTCAGGTGTCCATTGCCC
TAAGCTCTCACTGGATCAATCCTCGAAGAATGACCGACCACAGCATCAAAGAATGTCAAAAATCTCTGGACTTTGTACTAGGTTGGTTTGCCAAACCCGT
ATTTATTGATGGTGACTATCCCGAGAGCATGAAGAATAACCTTTCATCTATTCTGCCTGATTTTACTGAATCTGAGAAAAAGTTCATCAAAGGAACTGCT
GACTTTTTTGCTCTTTGCTTTGGACCCACCTTGAGTTTTCAACTTTTGGACCCTCACATGAAGTTCCGCCAATTGGAATCTCCCAACCTGAGGCAACTGC
TTTCCTGGATTGACCTTGAATTTAACCATCCTCAAATATTTATTGTGGAAAATGGCTGGTTTGTCTCAGGGACCACCAAGAGAGATGATGCCAAATATAT
GTATTACCTCAAAAAGTTCATCATGGAAACCTTAAAAGCCATCAAGCTGGATGGGGTGGATGTCATCGGGTATACCGCATGGTCCCTCATGGATGGTTTC
GAGTGGCACAGAGGTTACAGCATCAGGCGTGGACTCTTCTATGTTGACTTTCTAAGCCAGGACAAGATGTTGTTGCCAAAGTCTTCAGCCTTGTTCTACC
AAAAGCTGATAGAGAAAAATGGCTTCCCTCCTTTACCTGAAAATCAGCCCCTAGAAGGGACATTTCCCTGTGACTTTGCTTGGGGAGTTGTTGACAACTA
CATTCAAGTAGATACCACTCTGTCTCAGTTTACCGACCTGAATGTTTACCTGTGGGATGTCCACCACAGTAAAAGGCTTATTAAAGTGGATGGGGTTGTG
ACCAAGAAGAGGAAATCCTACTGTGTTGACTTTGCTGCCATCCAGCCCCAGATCGCTTTACTCCAGGAAATGCACGTTACACATTTTCGCTTCTCCCTGG
ACTGGGCCCTGATTCTCCCTCTGGGTAACCAGTCCCAGGTGAACCACACCATCCTGCAGTACTATCGCTGCATGGCCAGCGAGCTTGTCCGTGTCAACAT
CACCCCAGTGGTGGCCCTGTGGCAGCCTATGGCCCCGAACCAAGGACTGCCGCGCCTCCTGGCCAGGCAGGGCGCCTGGGAGAACCCCTACACTGCCCTG
GCCTTTGCAGAGTATGCCCGACTGTGCTTTCAAGAGCTCGGCCATCACGTCAAGCTTTGGATAACGATGAATGAGCCGTATACAAGGAATATGACATACA
GTGCTGGCCACAACCTTCTGAAGGCCCATGCCCTGGCTTGGCATGTGTACAATGAAAAGTTTAGGCATGCTCAGAATGGGAAAATATCCATAGCCTTGCA
GGCTGATTGGATAGAACCTGCCTGCCCTTTCTCCCAAAAGGACAAAGAGGTGGCTGAGAGAGTTTTGGAATTTGACATTGGCTGGCTGGCTGAGCCCATT
TTCGGCTCTGGAGATTATCCATGGGTGATGAGGGACTGGCTGAACCAAAGAAACAATTTTCTTCTTCCTTATTTCACTGAAGATGAAAAAAAGCTAATCC
AGGGTACCTTTGACTTTTTGGCTTTAAGCCATTATACCACCATCCTTGTAGACTCAGAAAAAGAAGATCCAATAAAATACAATGATTACCTAGAAGTGCA
AGAAATGACCGACATCACGTGGCTCAACTCCCCCAGTCAGGTGGCGGTAGTGCCCTGGGGGTTGCGCAAAGTGCTGAACTGGCTGAAGTTCAAGTACGGA
GACCTCCCCATGTACATAATATCCAATGGAATCGATGACGGGCTGCATGCTGAGGACGACCAGCTGAGGGTGTATTATATGCAGAATTACATAAACGAAG
CTCTCAAAGCCCACATACTGGATGGTATCAATCTTTGCGGATACTTTGCTTATTCGTTTAACGACCGCACAGCTCCGAGGTTTGGCCTCTATCGTTATGC
TGCAGATCAGTTTGAGCCCAAGGCATCCATGAAACATTACAGGAAAATTATTGACAGCAATGGTTTCCCGGGCCCAGAAACTCTGGAAAGATTTTGTCCA
GAAGAATTCACCGTGTGTACTGAGTGCAGTTTTTTTCACACCCGAAAGTCTTTACTGGCTTTCATAGCTTTTCTATTTTTTGCTTCTATTATTTCTCTCT
CCCTTATATTTTACTACTCGAAGAAAGGCAGAAGAAGTTACAAATAGTTCTGAACATTTTTCTATTCATTCATTTTGAAATAATTATGCAGACACATCAG
CTGTTAACCATTTGCACCTCTAAGTGTTGTGAAACTGTAAATTTCATACATTTGACTTCTAGAAAACATTTTTGTGGCTTATGACAGAGGTTTTGAAATG
GGCATAGGTGATCGTAAAATATTGAATAATGCGAATAGTGCCTGAATTTGTTCTCTTTTTGGGTGATTAAAAAACTGACAGGCACTATAATTTCTGTAAC
ACACTAACAAAAGCATGAAAAATAGGAACCACACCAATGCAACATTTGTGCAGAAATTTGAATGACAAGATTAGGAATATTTTCTTCTGCACCCACTTCT
AAATTTAATGTTTTTCTGGAAGTAGTAATTGCAAGAGTTCGAATAGAAAGTTATGTACCAAGTAACCATTTCTCAGCTGCCATAATAATGCCTAGTGGCT
TCCCCTCTGTCAAATCTAGTTTCCTATGGAAAAGAAGATGGCAGATACAGGAGAGACGACAGAGGGTCCTAGGCTGGAATGTTCCTTTCGAAAGCAATGC
TTCTATCAAATACTAGTATTAATTTATGTATCTGGTTAATGACATACTTGGAGAGCAAATTATGGAAATGTGTATTTTATATGATTTTTGAGGTCCTGTC
TAAACCCTGTGTCCCTGAGGGATCTGTCTCACTGGCATCTTGTTGAGGGCCTTGCACATAGGAAACTTTTGATAAGTATCTGCGGAAAAACAAACATGAA
TCCTGTGATATTGGGCTCTTCAGGAAGCATAAAGCAATTGTGAAATACAGTATACCGCAGTGGCTCTAGGTGGAGGAAAGGAGGAAAAAGTGCTTATTAT
GTGCAACATTATGATTAATCTGATTATACACCATTTTTGAGCAGATCTTGGAATGAATGACATGACCTTTCCCTAGAGAATAAGGATGAAATAATCACTC
ATTCTATGAACAGTGACACTACTTTCTATTCTTTAGCTGTACTGTAATTTCTTTGAGTTGATAGTTTTACAAATTCTTAATAGGTTCAAAAGCAATCTGG
TCTGAATAACACTGGATTTGTTTCTGTGATCTCTGAGGTCTATTTTATGTTTTTGCTGCTACTTCTGTGGAAGTAGCTTTGAACTAGTTTTACTTTGAAC
TTTCACGCTGAAACATGCTAGTGATATCTAGAAAGGGCTAATTAGGTCTCATCCTTTAATGCCCCTTAAATAAGTCTTGCTGATTTTCAGACAGGGAAGT
CTCTCTATTACACTGGAGCTGTTTTATAGATAAGTCAATATTGTATCAGGCAAGATAAACCAATGTCATAACAGGCATTGCCAACCTCACTGACACAGGG
TCATAGTGTATAATAATATACTGTACTATATAATATATCATCTTTAGAGGTATGATTTTTTCATGAAAGATAAGCTTTTGGTAATATTCATTTTAAAGTG
GACTTATTAAAATTGGATGCTAGAGAATCAAGTTTATTTTATGTATATATTTTTCTGATTATAAGAGTAATATATGTTCATTGTAAAAATTTTTAAAACA
CAGAAACTATATGCAAAGAAAAAATAAAAATTATCTATAATCTCAGAACCCAGAAATAGCCACTATTAACATTTCCTACGTATTTTATTTTACATAGATC
ATATTGTATATAGTTAGTATCTTTATTAATTTTTATTATGAAACTTTCCTTTGTCATTATTAGTCTTCAAAAGCATGATTTTTAATAGTTGTTGAGTATT
CCACCACAGGAATGTATCACAACTTAACCGTTCCCGTTTGTTAGACTAGTTTCTTATTAATGTTGATGAATGTTGTTTAAAAATAATTTTGTTGCTACAT
TTACTTTAATTTCCTTGACTGTAAAGAGAAGTAATTTTGCTCCTTGATAAAGTATTATATTAATAATAAATCTGCCTGCAACTTTTTGCCTTCTTTCATA
ATC
  • refseq_KL.F2 (forward primer)
  • refseq_KL.R2 (reverse primer)
  • Alternative donor and acceptor

Frame 0

RAACPPAPRRAARGRRRRRCRCCWCCWAWAAAA
CVRSRATARRPGPVSRGLLPPRPRASSRAPSPTA
SSGPWAAPPTRPRAAGSSTARVRPSGIRSPTTP
WHPRETPGTPVCRWAPRRRCSPPPGT*PATATT
TSSATRRRCASSGSLTTASPSRGRECSPMAARAS
PTARGCATTGACWSGCGSWACSPWSPCTTGTCP
SACRTPTAAGPTAPWPTTSGITRSSASATSAVR
SSTGSPSTTPTWWPGTATPPGAWPPASGAARGSG
TWWRTTSSWLMPKSGISTILLSVPLREVRCPLP
*ALTGSILEE*PTTASKNVKNLWTLY*VGLPNP
YLLMVTIPRA*RITFHLFCLILLNLRKSSSKELL
TFLLFALDPP*VFNFWTLT*SSANWNLPT*GNC
FPGLTLNLTILKYLLWKMAGLSQGPPREMMPNI
CITSKSSSWKP*KPSSWMGWMSSGIPHGPSWMVS
SGTEVTASGVDSSMLTF*ARTRCCCQSLQPCST
KS**RKMASLLYLKISP*KGHFPVTLLGELLTT
TFK*IPLCLSLPT*MFTCGMSTTVKGLLKWMGL*
PRRGNPTVLTLLPSSPRSLYSRKCTLHIFASPW
TGP*FSLWVTSPR*TTPSCSTIAAWPASLSVST
SPQWWPCGSLWPRTKDCRASWPGRAPGRTPTLPW
PLQSMPDCAFKSSAITSSFG*R*MSRIQGI*HT
VLATTF*RPMPWLGMCTMKSLGMLRMGKYP*PC
RLIG*NLPALSPKRTKRWLREFWNLTLAGWLSPF
SALEIIHG**GTG*TKETIFFFLISLKMKKS*S
RVPLTFWL*AIIPPSL*TQKKKIQ*NTMIT*KC
KK*PTSRGSTPPVRWR*CPGGCAKC*TG*SSSTE
TSPCT*YPMESMTGCMLRTTS*GCIICRIT*TK
LSKPTYWMVSIFADTLLIRLTTAQLRGLASIVM
LQISLSPRHP*NITGKLLTAMVSRAQKLWKDFVQ
KNSPCVLSAVFFTPESLYWLS*LFYFLLLLFLS
PLYFTTRRKAEEVTNSSEHFSIHSF*NNYADTS
AVNHLHL*VL*NCKFHTFDF*KTFLWLMTEVLKW
A*VIVKY*IMRIVPEFVLFLGD*KTDRHYNFCN
TLTKA*KIGTTPMQHLCRNLNDKIRNIFFCTHF
*I*CFSGSSNCKSSNRKLCTK*PFLSCHNNA*WL
PLCQI*FPMEKKMADTGETTEGPRLECSFRKQC
FYQILVLIYVSG**HTWRANYGNVYFI*FLRSC
LNPVSLRDLSHWHLVEGLAHRKLLISICGKTNMN
PVILGSSGSIKQL*NTVYRSGSRWRKGGKSAYY
VQHYD*SDYTPFLSRSWNE*HDLSLENKDEIIT
HSMNSDTTFYSLAVL*FL*VDSFTNS**VQKQSG
LNNTGFVSVISEVYFMFLLLLLWK*L*TSFTLN
FHAETC**YLERAN*VSSFNAP*ISLADFQTGK
SLYYTGAVL*ISQYCIRQDKPMS*QALPTSLTQG
HSV**YTVLYNISSLEV*FFHER*AFGNIHFKV
DLLKLDARESSLFYVYIFLIIRVIYVHCKNF*N
TETICKEKIKIIYNLRTQK*PLLTFPTYFILHRS
YCI*LVSLLIFIMKLSFVIISLQKHDF**LLSI
PPQECITT*PFPFVRLVSY*C**MLFKNNFVAT
FTLISLTVKRSNFAP**SIILIINLPATFCLLS*

Frame 1

AQHARQRPAAPPAAAAAVAVAAAGAAGPGRPPP
ACGAGRRRADLGPFLAASCPRGRGPLPGHLPRR
LPLGRGQRRLPDRGRLAAARQGCVHLGYVHPPPP
GTPGRLPERQSAVGRPVAAAARHRGRSQRQLQQ
RLPRHGGAARARGHSLPLLHLVGASAPQWQRGR
PQPRGAALLPAPAGAAAGAGRAARGHPVPLGPAP
APAGRLRRLGQPRPGRPLQGLRGALLPPLRRSG
QVLDHHRQPLRGGLARLRHRAPGPRHPGQPAAR
VPGGAQPPPGSCQSLASLQYFFPSHSGRSGVHCP
KLSLDQSSKNDRPQHQRMSKISGLCTRLVCQTR
IY*W*LSREHEE*PFIYSA*FY*I*EKVHQRNC
*LFCSLLWTHLEFSTFGPSHEVPPIGISQPEATA
FLD*P*I*PSSNIYCGKWLVCLRDHQER*CQIY
VLPQKVHHGNLKSHQAGWGGCHRVYRMVPHGWF
RVAQRLQHQAWTLLC*LSKPGQDVVAKVFSLVLP
KADREKWLPSFT*KSAPRRDISL*LCLGSC*QL
HSSRYHSVSVYRPECLPVGCPPQ*KAY*SGWGC
DQEEEILLC*LCCHPAPDRFTPGNARYTFSLLPG
LGPDSPSG*PVPGEPHHPAVLSLHGQRACPCQH
HPSGGPVAAYGPEPRTAAPPGQAGRLGEPLHCP
GLCRVCPTVLSRARPSRQALDNDE*AVYKEYDIQ
CWPQPSEGPCPGLACVQ*KV*ACSEWENIHSLA
G*LDRTCLPFLPKGQRGG*ESFGI*HWLAG*AH
FRLWRLSMGDEGLAEPKKQFSSSLFH*R*KKANP
GYL*LFGFKPLYHHPCRLRKRRSNKIQ*LPRSA
RNDRHHVAQLPQSGGGSALGVAQSAELAEVQVR
RPPHVHNIQWNR*RAAC*GRPAEGVLYAELHKRS
SQSPHTGWYQSLRILCLFV*RPHSSEVWPLSLC
CRSV*AQGIHETLQENY*QQWFPGPRNSGKILS
RRIHRVY*VQFFSHPKVFTGFHSFSIFCFYYFSL
PYILLLEERQKKLQIVLNIFLFIHFEIIMQTHQ
LLTICTSKCCETVNFIHLTSRKHFCGL*QRF*N
GHR*S*NIE*CE*CLNLFSFWVIKKLTGTIISVT
H*QKHEK*EPHQCNICAEI*MTRLGIFSSAPTS
KFNVFLEVVIARVRIESYVPSNHFSAAIIMPSG
FPSVKSSFLWKRRWQIQERRQRVLGWNVPFESNA
SIKY*Y*FMYLVNDILGEQIMEMCILYDF*GPV
*TLCP*GICLTGILLRALHIGNF**VSAEKQT*
IL*YWALQEA*SNCEIQYTAVALGGGKEEKVLIM
CNIMINLIIHHF*ADLGMNDMTFP*RIRMK*SL
IL*TVTLLSIL*LYCNFFELIVLQILNRFKSNL
V*ITLDLFL*SLRSILCFCCYFCGSSFELVLL*T
FTLKHASDI*KGLIRSHPLMPLK*VLLIFRQGS
LSITLELFYR*VNIVSGKINQCHNRHCQPH*HR
VIVYNNILYYIIYHL*RYDFFMKDKLLVIFILKW
TY*NWMLENQVYFMYIFF*L*E*YMFIVKIFKT
QKLYAKKK*KLSIISEPRNSHY*HFLRILFYID
HIVYS*YLY*FLL*NFPLSLLVFKSMIFNSC*VF
HHRNVSQLNRSRLLD*FLINVDECCLKIILLLH
LL*FP*L*REVILLLDKVLY***ICLQLFAFFH
N

Frame 2

RSMPASAPPRRPRPPPPSLSLLLVLLGLGGRRL
RAEPGDGAQTWARFSRPPAPEAAGLFQGTFPDG
FLWAVGSAAYQTEGGWQQHGKGASIWDTFTHHP
LAPPGDSRNASLPLGAPSPLQPATGDVASDSYNN
VFRDTEALRELGVTHYRFSISWARVLPNGSAGV
PNREGLRYYRRLLERLRELGVQPVVTLYHWDLP
QRLQDAYGGWANRALADHFRDYAELCFRHFGGQV
KYWITIDNPYVVAWHGYATGRLAPGIRGSPRLG
YLVAHNLLLAHAKVWHLYNTSFRPTQGGQVSIA
LSSHWINPRRMTDHSIKECQKSLDFVLGWFAKPV
FIDGDYPESMKNNLSSILPDFTESEKKFIKGTA
DFFALCFGPTLSFQLLDPHMKFRQLESPNLRQL
LSWIDLEFNHPQIFIVENGWFVSGTTKRDDAKYM
YYLKKFIMETLKAIKLDGVDVIGYTAWSLMDGF
EWHRGYSIRRGLFYVDFLSQDKMLLPKSSALFY
QKLIEKNGFPPLPENQPLEGTFPCDFAWGVVDNY
IQVDTTLSQFTDLNVYLWDVHHSKRLIKVDGVV
TKKRKSYCVDFAAIQPQIALLQEMHVTHFRFSL
DWALILPLGNQSQVNHTILQYYRCMASELVRVNI
TPVVALWQPMAPNQGLPRLLARQGAWENPYTAL
AFAEYARLCFQELGHHVKLWITMNEPYTRNMTY
SAGHNLLKAHALAWHVYNEKFRHAQNGKISIALQ
ADWIEPACPFSQKDKEVAERVLEFDIGWLAEPI
FGSGDYPWVMRDWLNQRNNFLLPYFTEDEKKLI
QGTFDFLALSHYTTILVDSEKEDPIKYNDYLEVQ
EMTDITWLNSPSQVAVVPWGLRKVLNWLKFKYG
DLPMYIISNGIDDGLHAEDDQLRVYYMQNYINE
ALKAHILDGINLCGYFAYSFNDRTAPRFGLYRYA
ADQFEPKASMKHYRKIIDSNGFPGPETLERFCP
EEFTVCTECSFFHTRKSLLAFIAFLFFASIISL
SLIFYYSKKGRRSYK*F*TFFYSFILK*LCRHIS
C*PFAPLSVVKL*ISYI*LLENIFVAYDRGFEM
GIGDRKILNNANSA*ICSLFG*LKN*QAL*FL*
HTNKSMKNRNHTNATFVQKFE*QD*EYFLLHPLL
NLMFFWK**LQEFE*KVMYQVTISQLP**CLVA
SPLSNLVSYGKEDGRYRRDDRGS*AGMFLSKAM
LLSNTSINLCIWLMTYLESKLWKCVFYMIFEVLS
KPCVPEGSVSLASC*GPCT*ETFDKYLRKNKHE
SCDIGLFRKHKAIVKYSIPQWL*VEERRKKCLL
CATL*LI*LYTIFEQILE*MT*PFPRE*G*NNHS
FYEQ*HYFLFFSCTVISLS**FYKFLIGSKAIW
SE*HWICFCDL*GLFYVFAATSVEVALN*FYFE
LSR*NMLVISRKG*LGLIL*CPLNKSC*FSDREV
SLLHWSCFIDKSILYQAR*TNVITGIANLTDTG
S*CIIIYCTI*YIIFRGMIFS*KISFW*YSF*S
GLIKIGC*RIKFILCIYFSDYKSNICSL*KFLKH
RNYMQRKNKNYL*SQNPEIATINISYVFYFT*I
ILYIVSIFINFYYETFLCHY*SSKA*FLIVVEY
STTGMYHNLTVPVC*TSFLLMLMNVV*K*FCCYI
YFNFLDCKEK*FCSLIKYYINNKSACNFLPSFI
I

Sequence NM_153683

CGCGCAGCATGCCCGCCAGCGCCCCGCCGCGCCGCCCGCGGCCGCCGCCGCCGTCGCTGTCGCTGCTGCTGGTGCTGCTGGGCCTGGGCGGCCGCCGCCT
GCGTGCGGAGCCGGGCGACGGCGCGCAGACCTGGGCCCGTTTCTCGCGGCCTCCTGCCCCCGAGGCCGCGGGCCTCTTCCAGGGCACCTTCCCCGACGGC
TTCCTCTGGGCCGTGGGCAGCGCCGCCTACCAGACCGAGGGCGGCTGGCAGCAGCACGGCAAGGGTGCGTCCATCTGGGATACGTTCACCCACCACCCCC
TGGCACCCCCGGGAGACTCCCGGAACGCCAGTCTGCCGTTGGGCGCCCCGTCGCCGCTGCAGCCCGCCACCGGGGACGTAGCCAGCGACAGCTACAACAA
CGTCTTCCGCGACACGGAGGCGCTGCGCGAGCTCGGGGTCACTCACTACCGCTTCTCCATCTCGTGGGCGCGAGTGCTCCCCAATGGCAGCGCGGGCGTC
CCCAACCGCGAGGGGCTGCGCTACTACCGGCGCCTGCTGGAGCGGCTGCGGGAGCTGGGCGTGCAGCCCGTGGTCACCCTGTACCACTGGGACCTGCCCC
AGCGCCTGCAGGACGCCTACGGCGGCTGGGCCAACCGCGCCCTGGCCGACCACTTCAGGGATTACGCGGAGCTCTGCTTCCGCCACTTCGGCGGTCAGGT
CAAGTACTGGATCACCATCGACAACCCCTACGTGGTGGCCTGGCACGGCTACGCCACCGGGCGCCTGGCCCCCGGCATCCGGGGCAGCCCGCGGCTCGGG
TACCTGGTGGCGCACAACCTCCTCCTGGCTCATGCCAAAGTCTGGCATCTCTACAATACTTCTTTCCGTCCCACTCAGGGAGGTCAGGTGTCCATTGCCC
TAAGCTCTCACTGGATCAATCCTCGAAGAATGACCGACCACAGCATCAAAGAATGTCAAAAATCTCTGGACTTTGTACTAGGTTGGTTTGCCAAACCCGT
ATTTATTGATGGTGACTATCCCGAGAGCATGAAGAATAACCTTTCATCTATTCTGCCTGATTTTACTGAATCTGAGAAAAAGTTCATCAAAGGAACTGCT
GACTTTTTTGCTCTTTGCTTTGGACCCACCTTGAGTTTTCAACTTTTGGACCCTCACATGAAGTTCCGCCAATTGGAATCTCCCAACCTGAGGCAACTGC
TTTCCTGGATTGACCTTGAATTTAACCATCCTCAAATATTTATTGTGGAAAATGGCTGGTTTGTCTCAGGGACCACCAAGAGAGATGATGCCAAATATAT
GTATTACCTCAAAAAGTTCATCATGGAAACCTTAAAAGCCATCAAGCTGGATGGGGTGGATGTCATCGGGTATACCGCATGGTCCCTCATGGATGGTTTC
GAGTGGCACAGAGGTTACAGCATCAGGCGTGGACTCTTCTATGTTGACTTTCTAAGCCAGGACAAGATGTTGTTGCCAAAGTCTTCAGCCTTGTTCTACC
AAAAGCTGATAGAGAAAAATGGCTTCCCTCCTTTACCTGAAAATCAGCCCCTAGAAGGGACATTTCCCTGTGACTTTGCTTGGGGAGTTGTTGACAACTA
CATTCAAGTAAGTCAGCTGACAAAACCAATCAGCAGTCTCACCAAGCCCTATCACTAATACCACTCTGTCTCAGTTTACCGACCTGAATGTTTACCTGTG
GGATGTCCACCACAGTAAAAGGCTTATTAAAGTGGATGGGGTTGTGACCAAGAAGAGGAAATCCTACTGTGTTGACTTTGCTGCCATCCAGCCCCAGATC
GCTTTACTCCAGG
AAATGCACGTTACACATTTTCGCTTCTCCCTGGACTGGGCCCTGATTCTCCCTCTGGGTAACCAGTCCCAGGTGAACCACACCATCC
TGCAGTACTATCGCTGCATGGCCAGCGAGCTTGTCCGTGTCAACATCACCCCAGTGGTGGCCCTGTGGCAGCCTATGGCCCCGAACCAAGGACTGCCGCG
CCTCCTGGCCAGGCAGGGCGCCTGGGAGAACCCCTACACTGCCCTGGCCTTTGCAGAGTATGCCCGACTGTGCTTTCAAGAGCTCGGCCATCACGTCAAG
CTTTGGATAACGATGAATGAGCCGTATACAAGGAATATGACATACAGTGCTGGCCACAACCTTCTGAAGGCCCATGCCCTGGCTTGGCATGTGTACAATG
AAAAGTTTAGGCATGCTCAGAATGGGAAAATATCCATAGCCTTGCAGGCTGATTGGATAGAACCTGCCTGCCCTTTCTCCCAAAAGGACAAAGAGGTGGC
TGAGAGAGTTTTGGAATTTGACATTGGCTGGCTGGCTGAGCCCATTTTCGGCTCTGGAGATTATCCATGGGTGATGAGGGACTGGCTGAACCAAAGAAAC
AATTTTCTTCTTCCTTATTTCACTGAAGATGAAAAAAAGCTAATCCAGGGTACCTTTGACTTTTTGGCTTTAAGCCATTATACCACCATCCTTGTAGACT
CAGAAAAAGAAGATCCAATAAAATACAATGATTACCTAGAAGTGCAAGAAATGACCGACATCACGTGGCTCAACTCCCCCAGTCAGGTGGCGGTAGTGCC
CTGGGGGTTGCGCAAAGTGCTGAACTGGCTGAAGTTCAAGTACGGAGACCTCCCCATGTACATAATATCCAATGGAATCGATGACGGGCTGCATGCTGAG
GACGACCAGCTGAGGGTGTATTATATGCAGAATTACATAAACGAAGCTCTCAAAGCCCACATACTGGATGGTATCAATCTTTGCGGATACTTTGCTTATT
CGTTTAACGACCGCACAGCTCCGAGGTTTGGCCTCTATCGTTATGCTGCAGATCAGTTTGAGCCCAAGGCATCCATGAAACATTACAGGAAAATTATTGA
CAGCAATGGTTTCCCGGGCCCAGAAACTCTGGAAAGATTTTGTCCAGAAGAATTCACCGTGTGTACTGAGTGCAGTTTTTTTCACACCCGAAAGTCTTTA
CTGGCTTTCATAGCTTTTCTATTTTTTGCTTCTATTATTTCTCTCTCCCTTATATTTTACTACTCGAAGAAAGGCAGAAGAAGTTACAAATAGTTCTGAA
CATTTTTCTATTCATTCATTTTGAAATAATTATGCAGACACATCAGCTGTTAACCATTTGCACCTCTAAGTGTTGTGAAACTGTAAATTTCATACATTTG
ACTTCTAGAAAACATTTTTGTGGCTTATGACAGAGGTTTTGAAATGGGCATAGGTGATCGTAAAATATTGAATAATGCGAATAGTGCCTGAATTTGTTCT
CTTTTTGGGTGATTAAAAAACTGACAGGCACTATAATTTCTGTAACACACTAACAAAAGCATGAAAAATAGGAACCACACCAATGCAACATTTGTGCAGA
AATTTGAATGACAAGATTAGGAATATTTTCTTCTGCACCCACTTCTAAATTTAATGTTTTTCTGGAAGTAGTAATTGCAAGAGTTCGAATAGAAAGTTAT
GTACCAAGTAACCATTTCTCAGCTGCCATAATAATGCCTAGTGGCTTCCCCTCTGTCAAATCTAGTTTCCTATGGAAAAGAAGATGGCAGATACAGGAGA
GACGACAGAGGGTCCTAGGCTGGAATGTTCCTTTCGAAAGCAATGCTTCTATCAAATACTAGTATTAATTTATGTATCTGGTTAATGACATACTTGGAGA
GCAAATTATGGAAATGTGTATTTTATATGATTTTTGAGGTCCTGTCTAAACCCTGTGTCCCTGAGGGATCTGTCTCACTGGCATCTTGTTGAGGGCCTTG
CACATAGGAAACTTTTGATAAGTATCTGCGGAAAAACAAACATGAATCCTGTGATATTGGGCTCTTCAGGAAGCATAAAGCAATTGTGAAATACAGTATA
CCGCAGTGGCTCTAGGTGGAGGAAAGGAGGAAAAAGTGCTTATTATGTGCAACATTATGATTAATCTGATTATACACCATTTTTGAGCAGATCTTGGAAT
GAATGACATGACCTTTCCCTAGAGAATAAGGATGAAATAATCACTCATTCTATGAACAGTGACACTACTTTCTATTCTTTAGCTGTACTGTAATTTCTTT
GAGTTGATAGTTTTACAAATTCTTAATAGGTTCAAAAGCAATCTGGTCTGAATAACACTGGATTTGTTTCTGTGATCTCTGAGGTCTATTTTATGTTTTT
GCTGCTACTTCTGTGGAAGTAGCTTTGAACTAGTTTTACTTTGAACTTTCACGCTGAAACATGCTAGTGATATCTAGAAAGGGCTAATTAGGTCTCATCC
TTTAATGCCCCTTAAATAAGTCTTGCTGATTTTCAGACAGGGAAGTCTCTCTATTACACTGGAGCTGTTTTATAGATAAGTCAATATTGTATCAGGCAAG
ATAAACCAATGTCATAACAGGCATTGCCAACCTCACTGACACAGGGTCATAGTGTATAATAATATACTGTACTATATAATATATCATCTTTAGAGGTATG
ATTTTTTCATGAAAGATAAGCTTTTGGTAATATTCATTTTAAAGTGGACTTATTAAAATTGGATGCTAGAGAATCAAGTTTATTTTATGTATATATTTTT
CTGATTATAAGAGTAATATATGTTCATTGTAAAAATTTTTAAAACACAGAAACTATATGCAAAGAAAAAATAAAAATTATCTATAATCTCAGAACCCAGA
AATAGCCACTATTAACATTTCCTACGTATTTTATTTTACATAGATCATATTGTATATAGTTAGTATCTTTATTAATTTTTATTATGAAACTTTCCTTTGT
CATTATTAGTCTTCAAAAGCATGATTTTTAATAGTTGTTGAGTATTCCACCACAGGAATGTATCACAACTTAACCGTTCCCGTTTGTTAGACTAGTTTCT
TATTAATGTTGATGAATGTTGTTTAAAAATAATTTTGTTGCTACATTTACTTTAATTTCCTTGACTGTAAAGAGAAGTAATTTTGCTCCTTGATAAAGTA
TTATATTAATAATAAATCTGCCTGCAACTTTTTGCCTTCTTTCATAATC
  • refseq_KL.F2 (forward primer)
  • refseq_KL.R2 (reverse primer)
  • Alternative donor and acceptor

Frame 0

RAACPPAPRRAARGRRRRRCRCCWCCWAWAAAA
CVRSRATARRPGPVSRGLLPPRPRASSRAPSPTA
SSGPWAAPPTRPRAAGSSTARVRPSGIRSPTTP
WHPRETPGTPVCRWAPRRRCSPPPGT*PATATT
TSSATRRRCASSGSLTTASPSRGRECSPMAARAS
PTARGCATTGACWSGCGSWACSPWSPCTTGTCP
SACRTPTAAGPTAPWPTTSGITRSSASATSAVR
SSTGSPSTTPTWWPGTATPPGAWPPASGAARGSG
TWWRTTSSWLMPKSGISTILLSVPLREVRCPLP
*ALTGSILEE*PTTASKNVKNLWTLY*VGLPNP
YLLMVTIPRA*RITFHLFCLILLNLRKSSSKELL
TFLLFALDPP*VFNFWTLT*SSANWNLPT*GNC
FPGLTLNLTILKYLLWKMAGLSQGPPREMMPNI
CITSKSSSWKP*KPSSWMGWMSSGIPHGPSWMVS
SGTEVTASGVDSSMLTF*ARTRCCCQSLQPCST
KS**RKMASLLYLKISP*KGHFPVTLLGELLTT
TFK*VS*QNQSAVSPSPITNTTLSQFTDLNVYLW
DVHHSKRLIKVDGVVTKKRKSYCVDFAAIQPQI
ALLQ
EMHVTHFRFSLDWALILPLGNQSQVNHTI
LQYYRCMASELVRVNITPVVALWQPMAPNQGLPR
LLARQGAWENPYTALAFAEYARLCFQELGHHVK
LWITMNEPYTRNMTYSAGHNLLKAHALAWHVYN
EKFRHAQNGKISIALQADWIEPACPFSQKDKEVA
ERVLEFDIGWLAEPIFGSGDYPWVMRDWLNQRN
NFLLPYFTEDEKKLIQGTFDFLALSHYTTILVD
SEKEDPIKYNDYLEVQEMTDITWLNSPSQVAVVP
WGLRKVLNWLKFKYGDLPMYIISNGIDDGLHAE
DDQLRVYYMQNYINEALKAHILDGINLCGYFAY
SFNDRTAPRFGLYRYAADQFEPKASMKHYRKIID
SNGFPGPETLERFCPEEFTVCTECSFFHTRKSL
LAFIAFLFFASIISLSLIFYYSKKGRRSYK*F*
TFFYSFILK*LCRHISC*PFAPLSVVKL*ISYI*
LLENIFVAYDRGFEMGIGDRKILNNANSA*ICS
LFG*LKN*QAL*FL*HTNKSMKNRNHTNATFVQ
KFE*QD*EYFLLHPLLNLMFFWK**LQEFE*KVM
YQVTISQLP**CLVASPLSNLVSYGKEDGRYRR
DDRGS*AGMFLSKAMLLSNTSINLCIWLMTYLE
SKLWKCVFYMIFEVLSKPCVPEGSVSLASC*GPC
T*ETFDKYLRKNKHESCDIGLFRKHKAIVKYSI
PQWL*VEERRKKCLLCATL*LI*LYTIFEQILE
*MT*PFPRE*G*NNHSFYEQ*HYFLFFSCTVISL
S**FYKFLIGSKAIWSE*HWICFCDL*GLFYVF
AATSVEVALN*FYFELSR*NMLVISRKG*LGLI
L*CPLNKSC*FSDREVSLLHWSCFIDKSILYQAR
*TNVITGIANLTDTGS*CIIIYCTI*YIIFRGM
IFS*KISFW*YSF*SGLIKIGC*RIKFILCIYF
SDYKSNICSL*KFLKHRNYMQRKNKNYL*SQNPE
IATINISYVFYFT*IILYIVSIFINFYYETFLC
HY*SSKA*FLIVVEYSTTGMYHNLTVPVC*TSF
LLMLMNVV*K*FCCYIYFNFLDCKEK*FCSLIKY
YINNKSACNFLPSFII

Frame 1

AQHARQRPAAPPAAAAAVAVAAAGAAGPGRPPP
ACGAGRRRADLGPFLAASCPRGRGPLPGHLPRR
LPLGRGQRRLPDRGRLAAARQGCVHLGYVHPPPP
GTPGRLPERQSAVGRPVAAAARHRGRSQRQLQQ
RLPRHGGAARARGHSLPLLHLVGASAPQWQRGR
PQPRGAALLPAPAGAAAGAGRAARGHPVPLGPAP
APAGRLRRLGQPRPGRPLQGLRGALLPPLRRSG
QVLDHHRQPLRGGLARLRHRAPGPRHPGQPAAR
VPGGAQPPPGSCQSLASLQYFFPSHSGRSGVHCP
KLSLDQSSKNDRPQHQRMSKISGLCTRLVCQTR
IY*W*LSREHEE*PFIYSA*FY*I*EKVHQRNC
*LFCSLLWTHLEFSTFGPSHEVPPIGISQPEATA
FLD*P*I*PSSNIYCGKWLVCLRDHQER*CQIY
VLPQKVHHGNLKSHQAGWGGCHRVYRMVPHGWF
RVAQRLQHQAWTLLC*LSKPGQDVVAKVFSLVLP
KADREKWLPSFT*KSAPRRDISL*LCLGSC*QL
HSSKSADKTNQQSHQALSLIPLCLSLPT*MFTC
GMSTTVKGLLKWMGL*PRRGNPTVLTLLPSSPRS
LYSR
KCTLHIFASPWTGP*FSLWVTSPR*TTPS
CSTIAAWPASLSVSTSPQWWPCGSLWPRTKDCR
ASWPGRAPGRTPTLPWPLQSMPDCAFKSSAITSS
FG*R*MSRIQGI*HTVLATTF*RPMPWLGMCTM
KSLGMLRMGKYP*PCRLIG*NLPALSPKRTKRW
LREFWNLTLAGWLSPFSALEIIHG**GTG*TKET
IFFFLISLKMKKS*SRVPLTFWL*AIIPPSL*T
QKKKIQ*NTMIT*KCKK*PTSRGSTPPVRWR*C
PGGCAKC*TG*SSSTETSPCT*YPMESMTGCMLR
TTS*GCIICRIT*TKLSKPTYWMVSIFADTLLI
RLTTAQLRGLASIVMLQISLSPRHP*NITGKLL
TAMVSRAQKLWKDFVQKNSPCVLSAVFFTPESLY
WLS*LFYFLLLLFLSPLYFTTRRKAEEVTNSSE
HFSIHSF*NNYADTSAVNHLHL*VL*NCKFHTF
DF*KTFLWLMTEVLKWA*VIVKY*IMRIVPEFVL
FLGD*KTDRHYNFCNTLTKA*KIGTTPMQHLCR
NLNDKIRNIFFCTHF*I*CFSGSSNCKSSNRKL
CTK*PFLSCHNNA*WLPLCQI*FPMEKKMADTGE
TTEGPRLECSFRKQCFYQILVLIYVSG**HTWR
ANYGNVYFI*FLRSCLNPVSLRDLSHWHLVEGL
AHRKLLISICGKTNMNPVILGSSGSIKQL*NTVY
RSGSRWRKGGKSAYYVQHYD*SDYTPFLSRSWN
E*HDLSLENKDEIITHSMNSDTTFYSLAVL*FL
*VDSFTNS**VQKQSGLNNTGFVSVISEVYFMFL
LLLLWK*L*TSFTLNFHAETC**YLERAN*VSS
FNAP*ISLADFQTGKSLYYTGAVL*ISQYCIRQ
DKPMS*QALPTSLTQGHSV**YTVLYNISSLEV*
FFHER*AFGNIHFKVDLLKLDARESSLFYVYIF
LIIRVIYVHCKNF*NTETICKEKIKIIYNLRTQ
K*PLLTFPTYFILHRSYCI*LVSLLIFIMKLSFV
IISLQKHDF**LLSIPPQECITT*PFPFVRLVS
Y*C**MLFKNNFVATFTLISLTVKRSNFAP**S
IILIINLPATFCLLS*

Frame 2

RSMPASAPPRRPRPPPPSLSLLLVLLGLGGRRL
RAEPGDGAQTWARFSRPPAPEAAGLFQGTFPDG
FLWAVGSAAYQTEGGWQQHGKGASIWDTFTHHP
LAPPGDSRNASLPLGAPSPLQPATGDVASDSYNN
VFRDTEALRELGVTHYRFSISWARVLPNGSAGV
PNREGLRYYRRLLERLRELGVQPVVTLYHWDLP
QRLQDAYGGWANRALADHFRDYAELCFRHFGGQV
KYWITIDNPYVVAWHGYATGRLAPGIRGSPRLG
YLVAHNLLLAHAKVWHLYNTSFRPTQGGQVSIA
LSSHWINPRRMTDHSIKECQKSLDFVLGWFAKPV
FIDGDYPESMKNNLSSILPDFTESEKKFIKGTA
DFFALCFGPTLSFQLLDPHMKFRQLESPNLRQL
LSWIDLEFNHPQIFIVENGWFVSGTTKRDDAKYM
YYLKKFIMETLKAIKLDGVDVIGYTAWSLMDGF
EWHRGYSIRRGLFYVDFLSQDKMLLPKSSALFY
QKLIEKNGFPPLPENQPLEGTFPCDFAWGVVDNY
IQVSQLTKPISSLTKPYH*YHSVSVYRPECLPV
GCPPQ*KAY*SGWGCDQEEEILLC*LCCHPAPD
RFTPG
NARYTFSLLPGLGPDSPSG*PVPGEPHHP
AVLSLHGQRACPCQHHPSGGPVAAYGPEPRTAA
PPGQAGRLGEPLHCPGLCRVCPTVLSRARPSRQ
ALDNDE*AVYKEYDIQCWPQPSEGPCPGLACVQ*
KV*ACSEWENIHSLAG*LDRTCLPFLPKGQRGG
*ESFGI*HWLAG*AHFRLWRLSMGDEGLAEPKK
QFSSSLFH*R*KKANPGYL*LFGFKPLYHHPCRL
RKRRSNKIQ*LPRSARNDRHHVAQLPQSGGGSA
LGVAQSAELAEVQVRRPPHVHNIQWNR*RAAC*
GRPAEGVLYAELHKRSSQSPHTGWYQSLRILCLF
V*RPHSSEVWPLSLCCRSV*AQGIHETLQENY*
QQWFPGPRNSGKILSRRIHRVY*VQFFSHPKVF
TGFHSFSIFCFYYFSLPYILLLEERQKKLQIVLN
IFLFIHFEIIMQTHQLLTICTSKCCETVNFIHL
TSRKHFCGL*QRF*NGHR*S*NIE*CE*CLNLF
SFWVIKKLTGTIISVTH*QKHEK*EPHQCNICAE
I*MTRLGIFSSAPTSKFNVFLEVVIARVRIESY
VPSNHFSAAIIMPSGFPSVKSSFLWKRRWQIQE
RRQRVLGWNVPFESNASIKY*Y*FMYLVNDILGE
QIMEMCILYDF*GPV*TLCP*GICLTGILLRAL
HIGNF**VSAEKQT*IL*YWALQEA*SNCEIQY
TAVALGGGKEEKVLIMCNIMINLIIHHF*ADLGM
NDMTFP*RIRMK*SLIL*TVTLLSIL*LYCNFF
ELIVLQILNRFKSNLV*ITLDLFL*SLRSILCF
CCYFCGSSFELVLL*TFTLKHASDI*KGLIRSHP
LMPLK*VLLIFRQGSLSITLELFYR*VNIVSGK
INQCHNRHCQPH*HRVIVYNNILYYIIYHL*RY
DFFMKDKLLVIFILKWTY*NWMLENQVYFMYIFF
*L*E*YMFIVKIFKTQKLYAKKK*KLSIISEPR
NSHY*HFLRILFYIDHIVYS*YLY*FLL*NFPL
SLLVFKSMIFNSC*VFHHRNVSQLNRSRLLD*FL
INVDECCLKIILLLHLL*FP*L*REVILLLDKV
LY***ICLQLFAFFHN

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