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Sequences

Sequence NM_152298

refseq_NASP.F1 (forward primer)
refseq_NASP.R1 (reverse primer)
Single exon skipping

Frame 0

NLPFSVPE*VSGVPNCLFFSQALFRSLVRHLRG TMAMESTATAAVAAELVSADKIEDVPAPSTSADK VESLDVDSEAKKLLGLGQKHLVMGDIPAAVNAF QEAASLLGKKYGETANECGEAFFFYGKSLLELA RMENGVLGNALEGVHVEEEEGEKTEDESLVENND NIDETEGSEEDDKENDKTEEMPNDSVLENKSLQ ENEEEEIGNLELAWDMLDLAKIIFKRQETKEAQ LYAAQAHLKLGEVSVESENYVQAVEEFQSCLNLQ EQYLEAHDRLLAETHYQLGLAYGYNSQYDEAVA QFSKSIEVIENRMAVLNEQVKEAEGSSAEYKKE IEELKELLPEIREKIEDAKESQRSGNVAELALKA TLVESSTSGFTPGGGGSSVSMIASRKPTDGASS SNCVTDISHLVRKKRKPEEESPRKDDAKKAKQE PEVNGGSGDAVPSGNEVSENMEEEAENQAESRAA VEGTVEAGATVESTAC*EGAQPSSQGKVFLYIM YFFTFGGFFLYNFNKDCKQRLRL*WFFS*LLAE SALEHCWFYILAKGFVLAFCTDLCS*S*FLSV*R GGDQVWL*AFVFQWCYILFSNTSLNPAVLQTFS GAVPGWGLQKQELVKILLFSAENG*WTLAVSQA *DGSCPIST*SSPGAIILSWKKNSENLGQGNDS* GKRSAFELWFESSQGD*WWTLQMWLEAYVGRLA G*VLLFSV*KG*DISTLGIVTHLQN*LLK*RC* KS

Frame 1

ICHFLSLSESLASQIACFSRRLYSVRWFATSGE RWPWSPQPLPPSPRSWFLPTKLKMFLLLLHLQI KWRVWMWIVKLRNYWV*DRNIW*WGIFQQLSMHS RKQLVF*VRSMERQLMSVEKPSFSMGNHFWSWQ EWRMVCWETPWKVCMWKRKKEKKQKMNLW*KIM IT*MKLKAQKRMIKKMIRPKKCQMIQSLKTSLFK KMRRRRLGT*SLPGICWI*QRSFLKGKKQKKHS FMLPRHILNSEKLVLNLKTMCKLWRSSSPALTC RNSTWKPTTVSLQRPTTSWAWLMGTTLSMMRQWH SSANLLKSLRTEWLY*TSR*RRLKDRLLNTRKK LRN*RNCYPKLERR*KMQRSLSVVGM*LNWL*K LLWWRVLLQVSLLVEEALQSP*LPVESQQTVLPH QIV*LIFPTLSERRGNQRKRVPGKMMQRKPNKS RR*TEAVGMLSPVEMKFRKTWRRRLRIRLKAGQ QWRGQWRLELQLKALHVKRGHSPPPKGKCFCI*C IFSLLEDSFCITSIKIVSKG*GFDGFFLNYWLN LPWSTAGFIY*PKVLFWPSVLICVPDPNSYLSN VEVIKCGCRPLFSNGAIFCFQTLH*TQLSCKLSV VLSLDGGYKNKNW*RSCSSVLKMDDGLWL*ARP RMVLVLYPPSLHLGL*FCPGKRTLKTWVRGMIP KENGLHLSSGLKVAKGTDGGHSRCGWKHMWGGWL VEFCYFLYRKVEIYQHLELLPICRIDFSNKDAK NL

Frame 2

SAIFCP*VSLWRPKLPVFLAGSIPFAGSPPQGN DGHGVHSHCRRRRGAGFCRQN*RCSCSFYICR* SGESGCG**S*ETIGFRTETSGDGGYSSSCQCI PGSS*SFR*EVWRDS**VWRSLLFLWEITSGVGK NGEWCVGKRLGRCACGRGRRRKNRR*ISGRK** *HR*N*RLRRG**RK**DRRNAK*FSP*KQVSS RK*GGGDWEPRACLGYAGFSKDHF*KARNKRSTA LCCPGTS*TRRS*C*I*KLCASCGGVPVLP*PA GTVPGSPRPSPCRDPLPAGLGLWVQLSV**GSG TVQQIY*SH*EQNGCTKRAGEGG*RIVC*IQERN *GTKGTATRN*REDRRCKGVSA*WECS*TGSES YSGGEFYFRFHSWWRRLFSLHDCQ*KANRRCFL IKLCD*YFPPCQKEEETRGRESPER*CKESQTRA GGERRQWGCCPQWK*SFGKHGGGG*ESG*KPGS SGGDSGGWSYS*KHCMLRGGTALLPRESVFVYN VFFHFWRILFV*LQ*RL*AKVEALMVFFLIIG*I CLGALLVLYISQRFCSGLLY*SVFLILIPICLT WR*SSVAVGLCFPMVLYSVFKHFTEPSCLANFQ WCCPWMGATKTRIGEDLALQC*KWMMDFGCEPGL GWFLSYIHLVFTWGYNSVLEKEL*KPGSGE*FL RKTVCI*ALV*K*PRGLMVDTPDVVGSICGEAG WLSFVIFCIERLRYINTWNCYPSAELTSQIKMLK I

Sequence NM_002482

AATCTGCCATTTTCTGTCCCTGAGTGAGTCTCTGGCGTCCCAAATTGCCTGTTTTTCTCGCAGGCTCTATTCCGTTCGCTGGTTCGCCACCTCAGGGGAA CGATGGCCATGGAGTCCACAGCCACTGCCGCCGTCGCCGCGGAGCTGGTTTCTGCCGACAAAATTGAAGATGTTCCTGCTCCTTCTACATCTGCAGATAA AGTGGAGAGTCTGGATGTGGATAGTGAAGCTAAGAAACTATTGGGTTTAGGACAGAAACATCTGGTGATGGGGGATATTCCAGCAGCTGTCAATGCATTC CAGGAAGCAGCTAGTCTTTTAGGTAAGAAGTATGGAGAGACAGCTAATGAGTGTGGAGAAGCCTTCTTTTTCTATGGGAAATCACTTCTGGAGTTGGCAA GAATGGAGAATGGTGTGTTGGGAAACGCCTTGGAAGGTGTGCATGTGGAAGAGGAAGAAGGAGAAAAAACAGAAGATGAATCTCTGGTAGAAAATAATGA TAACATAGATGAGGAAGCAAGGGAAGAGTTGAGAGAACAGGTTTATGACGCCATGGGAGAAAAAGAAGAAGCCAAAAAAACAGAAGACAAGTCTTTGGCA AAGCCTGAAACTGATAAAGAACAGGACAGTGAAATGGAGAAGGGTGGAAGAGAAGATATGGATATAAGTAAATCTGCAGAGGAGCCACAGGAAAAAGTTG ACTTGACTCTAGATTGGTTAACTGAAACCTCTGAAGAGGCAAAAGGAGGAGCAGCACCAGAAGGACCGAATGAAGCTGAGGTCACTTCTGGGAAGCCAGA ACAGGAAGTACCAGATGCTGAGGAAGAAAAATCAGTTTCTGGAACTGATGTCCAAGAAGAGTGCAGAGAAAAAGGAGGTCAGGAGAAGCAGGGAGAGGTA ATTGTGAGCATAGAGGAGAAGCCAAAAGAAGTTTCAGAAGAGCAGCCTGTGGTGACTCTAGAAAAGCAGGGCACTGCAGTGGAGGTAGAAGCAGAGTCTT TAGACCCGACAGTCAAGCCAGTGGATGTGGGTGGGGACGAGCCAGAGGAGAAGGTAGTTACCTCTGAAAACGAGGCAGGAAAGGCGGTTCTTGAACAACT GGTAGGTCAAGAAGTACCACCTGCTGAAGAGTCACCAGAGGTGACAACAGAGGCTGCAGAGGCCTCAGCTGTAGAGGCTGGATCAGAAGTCTCTGAAAAG CCTGGGCAGGAGGCTCCAGTTCTCCCTAAGGATGGTGCAGTCAATGGACCGTCAGTTGTAGGAGATCAGACTCCTATTGAACCACAGACTTCTATAGAAA GACTGACAGAAACAAAAGATGGCTCAGGACTAGAGGAGAAGGTCAGGGCAAAGCTGGTTCCTAGTCAGGAGGAGACTAAGCTGTCTGTAGAAGAGTCTGA GGCAGCTGGAGATGGGGTTGATACCAAGGTAGCCCAGGGAGCTACTGAGAAATCACCTGAAGACAAAGTTCAGATAGCTGCTAATGAAGAGACACAAGAG AGAGAAGAACAGATGAAAGAGGGTGAAGAAACTGAAGGCTCAGAAGAGGATGATAAAGAAAATGATAAGACCGAAGAAATGCCAAATGATTCAGTCCTTG AAAACAAGTCTCTTCAAGAAAATGAGGAGGAGGAGATTGGGAACCTAGAGCTTGCCTGGGATATGCTGGATTTAGCAAAGATCATTTTTAAAAGGCAAGA AACAAAAGAAGCACAGCTTTATGCTGCCCAGGCACATCTTAAACTCGGAGAAGTTAGTGTTGAATCTGAAAACTATGTGCAAGCTGTGGAGGAGTTCCAG TCCTGCCTTAACCTGCAGGAACAGTACCTGGAAGCCCACGACCGTCTCCTTGCAGAGACCCACTACCAGCTGGGCTTGGCTTATGGGTACAACTCTCAGT ATGATGAGGCAGTGGCACAGTTCAGCAAATCTATTGAAGTCATTGAGAACAGAATGGCTGTACTAAACGAGCAGGTGAAGGAGGCTGAAGGATCGTCTGC TGAATACAAGAAAGAAATTGAGGAACTAAAGGAACTGCTACCCGAAATTAGAGAGAAGATAGAAGATGCAAAGGAGTCTCAGCGTAGTGGGAATGTAGCT GAACTGGCTCTGAAAGCTACTCTGGTGGAGAGTTCTACTTCAGGTTTCACTCCTGGTGGAGGAGGCTCTTCAGTCTCCATGATTGCCAGTAGAAAGCCAA CAGACGGTGCTTCCTCATCAAATTGTGTGACTGATATTTCCCACCTTGTCAGAAAGAAGAGGAAACCAGAGGAAGAGAGTCCCCGGAAAGATGATGCAAA GAAAGCCAAACAAGAGCCGGAGGTGAACGGAGGCAGTGGGGATGCTGTCCCCAGTGGAAATGAAGTTTCGGAAAACATGGAGGAGGAGGCTGAGAATCAG GCTGAAAGCCGGGCAGCAGTGGAGGGGACAGTGGAGGCTGGAGCTACAGTTGAAAGCACTGCATGTTAAGAGGGGGCACAGCCCTCCTCCCAAGGGAAAG TGTTTTTGTATATAATGTATTTTTTCACTTTTGGAGGATTCTTTTTGTATAACTTCAATAAAGATTGTAAGCAAAGGTTGAGGCTTTGATGGTTTTTTTC TTAATTATTGGCTGAATCTGCCTTGGAGCACTGCTGGTTTTATATATTAGCCAAAGGTTTTGTTCTGGCCTTCTGTACTGATCTGTGTTCCTGATCCTAA TTCCTATCTGTCTAACGTGGAGGTGATCAAGTGTGGCTGTAGGCCTTTGTTTTCCAATGGTGCTATATTCTGTTTTCAAACACTTCACTGAACCCAGCTG TCTTGCAAACTTTCAGTGGTGCTGTCCCTGGATGGGGGCTACAAAAACAAGAATTGGTGAAGATCTTGCTCTTCAGTGCTGAAAATGGATGATGGACTTT GGCTGTGAGCCAGGCCTAGGATGGTTCTTGTCCTATATCCACCTAGTCTTCACCTGGGGCTATAATTCTGTCCTGGAAAAAGAACTCTGAAAACCTGGGT CAGGGGAATGATTCCTAAGGAAAACGGTCTGCATTTGAGCTCTGGTTTGAAAGTAGCCAAGGGGACTGATGGTGGACACTCCAGATGTGGTTGGAAGCAT ATGTGGGGAGGCTGGCTGGTTGAGTTTTGTTATTTTCTGTATAGAAAGGTTGAGATATATCAACACTTGGAATTGTTACCCATCTGCAGAATTGACTTCT CAAATAAAGATGCTAAAAATCTC

refseq_NASP.F1 (forward primer)
refseq_NASP.R1 (reverse primer)
Single exon skipping

Frame 0

NLPFSVPE*VSGVPNCLFFSQALFRSLVRHLRG TMAMESTATAAVAAELVSADKIEDVPAPSTSADK VESLDVDSEAKKLLGLGQKHLVMGDIPAAVNAF QEAASLLGKKYGETANECGEAFFFYGKSLLELA RMENGVLGNALEGVHVEEEEGEKTEDESLVENND NIDEEAREELREQVYDAMGEKEEAKKTEDKSLA KPETDKEQDSEMEKGGREDMDISKSAEEPQEKV DLTLDWLTETSEEAKGGAAPEGPNEAEVTSGKPE QEVPDAEEEKSVSGTDVQEECREKGGQEKQGEV IVSIEEKPKEVSEEQPVVTLEKQGTAVEVEAES LDPTVKPVDVGGDEPEEKVVTSENEAGKAVLEQL VGQEVPPAEESPEVTTEAAEASAVEAGSEVSEK PGQEAPVLPKDGAVNGPSVVGDQTPIEPQTSIE RLTETKDGSGLEEKVRAKLVPSQEETKLSVEESE AAGDGVDTKVAQGATEKSPEDKVQIAANEETQE REEQMKEGEETEGSEEDDKENDKTEEMPNDSVL ENKSLQENEEEEIGNLELAWDMLDLAKIIFKRQE TKEAQLYAAQAHLKLGEVSVESENYVQAVEEFQ SCLNLQEQYLEAHDRLLAETHYQLGLAYGYNSQ YDEAVAQFSKSIEVIENRMAVLNEQVKEAEGSSA EYKKEIEELKELLPEIREKIEDAKESQRSGNVA ELALKATLVESSTSGFTPGGGGSSVSMIASRKP TDGASSSNCVTDISHLVRKKRKPEEESPRKDDAK KAKQEPEVNGGSGDAVPSGNEVSENMEEEAENQ AESRAAVEGTVEAGATVESTAC*EGAQPSSQGK VFLYIMYFFTFGGFFLYNFNKDCKQRLRL*WFFS *LLAESALEHCWFYILAKGFVLAFCTDLCS*S* FLSV*RGGDQVWL*AFVFQWCYILFSNTSLNPA VLQTFSGAVPGWGLQKQELVKILLFSAENG*WTL AVSQA*DGSCPIST*SSPGAIILSWKKNSENLG QGNDS*GKRSAFELWFESSQGD*WWTLQMWLEA YVGRLAG*VLLFSV*KG*DISTLGIVTHLQN*LL K*RC*KS

Frame 1

ICHFLSLSESLASQIACFSRRLYSVRWFATSGE RWPWSPQPLPPSPRSWFLPTKLKMFLLLLHLQI KWRVWMWIVKLRNYWV*DRNIW*WGIFQQLSMHS RKQLVF*VRSMERQLMSVEKPSFSMGNHFWSWQ EWRMVCWETPWKVCMWKRKKEKKQKMNLW*KIM IT*MRKQGKS*ENRFMTPWEKKKKPKKQKTSLWQ SLKLIKNRTVKWRRVEEKIWI*VNLQRSHRKKL T*L*IG*LKPLKRQKEEQHQKDRMKLRSLLGSQ NRKYQMLRKKNQFLELMSKKSAEKKEVRRSRER* L*A*RRSQKKFQKSSLW*L*KSRALQWR*KQSL *TRQSSQWMWVGTSQRRR*LPLKTRQERRFLNN W*VKKYHLLKSHQR*QQRLQRPQL*RLDQKSLKS LGRRLQFSLRMVQSMDRQL*EIRLLLNHRLL*K D*QKQKMAQD*RRRSGQSWFLVRRRLSCL*KSL RQLEMGLIPR*PRELLRNHLKTKFR*LLMKRHKR EKNR*KRVKKLKAQKRMIKKMIRPKKCQMIQSL KTSLFKKMRRRRLGT*SLPGICWI*QRSFLKGK KQKKHSFMLPRHILNSEKLVLNLKTMCKLWRSSS PALTCRNSTWKPTTVSLQRPTTSWAWLMGTTLS MMRQWHSSANLLKSLRTEWLY*TSR*RRLKDRL LNTRKKLRN*RNCYPKLERR*KMQRSLSVVGM*L NWL*KLLWWRVLLQVSLLVEEALQSP*LPVESQ QTVLPHQIV*LIFPTLSERRGNQRKRVPGKMMQ RKPNKSRR*TEAVGMLSPVEMKFRKTWRRRLRIR LKAGQQWRGQWRLELQLKALHVKRGHSPPPKGK CFCI*CIFSLLEDSFCITSIKIVSKG*GFDGFF LNYWLNLPWSTAGFIY*PKVLFWPSVLICVPDPN SYLSNVEVIKCGCRPLFSNGAIFCFQTLH*TQL SCKLSVVLSLDGGYKNKNW*RSCSSVLKMDDGL WL*ARPRMVLVLYPPSLHLGL*FCPGKRTLKTWV RGMIPKENGLHLSSGLKVAKGTDGGHSRCGWKH MWGGWLVEFCYFLYRKVEIYQHLELLPICRIDF SNKDAKNL

Frame 2

SAIFCP*VSLWRPKLPVFLAGSIPFAGSPPQGN DGHGVHSHCRRRRGAGFCRQN*RCSCSFYICR* SGESGCG**S*ETIGFRTETSGDGGYSSSCQCI PGSS*SFR*EVWRDS**VWRSLLFLWEITSGVGK NGEWCVGKRLGRCACGRGRRRKNRR*ISGRK** *HR*GSKGRVERTGL*RHGRKRRSQKNRRQVFG KA*N**RTGQ*NGEGWKRRYGYK*ICRGATGKS* LDSRLVN*NL*RGKRRSSTRRTE*S*GHFWEAR TGSTRC*GRKISFWN*CPRRVQRKRRSGEAGRG NCEHRGEAKRSFRRAACGDSRKAGHCSGGRSRVF RPDSQASGCGWGRARGEGSYL*KRGRKGGS*TT GRSRSTTC*RVTRGDNRGCRGLSCRGWIRSL*K AWAGGSSSP*GWCSQWTVSCRRSDSY*TTDFYRK TDRNKRWLRTRGEGQGKAGS*SGGD*AVCRRV* GSWRWG*YQGSPGSY*EIT*RQSSDSC**RDTR ERRTDERG*RN*RLRRG**RK**DRRNAK*FSP* KQVSSRK*GGGDWEPRACLGYAGFSKDHF*KAR NKRSTALCCPGTS*TRRS*C*I*KLCASCGGVP VLP*PAGTVPGSPRPSPCRDPLPAGLGLWVQLSV **GSGTVQQIY*SH*EQNGCTKRAGEGG*RIVC *IQERN*GTKGTATRN*REDRRCKGVSA*WECS *TGSESYSGGEFYFRFHSWWRRLFSLHDCQ*KAN RRCFLIKLCD*YFPPCQKEEETRGRESPER*CK ESQTRAGGERRQWGCCPQWK*SFGKHGGGG*ES G*KPGSSGGDSGGWSYS*KHCMLRGGTALLPRES VFVYNVFFHFWRILFV*LQ*RL*AKVEALMVFF LIIG*ICLGALLVLYISQRFCSGLLY*SVFLIL IPICLTWR*SSVAVGLCFPMVLYSVFKHFTEPSC LANFQWCCPWMGATKTRIGEDLALQC*KWMMDF GCEPGLGWFLSYIHLVFTWGYNSVLEKEL*KPG SGE*FLRKTVCI*ALV*K*PRGLMVDTPDVVGSI CGEAGWLSFVIFCIERLRYINTWNCYPSAELTS QIKMLKI

Sequence NM_172164

AATCTGCCATTTTCTGTCCCTGAGTGAGTCTCTGGCGTCCCAAATTGCCTGTTTTTCTCGCAGGCTCTATTCCGTTCGCTGGTTCGCCACCTCAGGGGAA CGATGGCCATGGAGTCCACAGCCACTGCCGCCGTCGCCGCGGAGCTGGTTTCTGCCGACAAAATTGAAGATGTTCCTGCTCCTTCTACATCTGCAGATAA AGTGGAGAGCAACCATTAATCTGCTTTCTGTCACTGAAGATGGGTTGCATTTTGTAGAATATTATCTAAATAGAATCATACATCTGGATGTGGATAGTGA AGCTAAGAAACTATTGGGTTTAGGACAGAAACATCTGGTGATGGGGGATATTCCAGCAGCTGTCAATGCATTCCAGGAAGCAGCTAGTCTTTTAGGTAAG AAGTATGGAGAGACAGCTAATGAGTGTGGAGAAGCCTTCTTTTTCTATGGGAAATCACTTCTGGAGTTGGCAAGAATGGAGAATGGTGTGTTGGGAAACG CCTTGGAAGGTGTGCATGTGGAAGAGGAAGAAGGAGAAAAAACAGAAGATGAATCTCTGGTAGAAAATAATGATAACATAGATGAGGAAGCAAGGGAAGA GTTGAGAGAACAGGTTTATGACGCCATGGGAGAAAAAGAAGAAGCCAAAAAAACAGAAGACAAGTCTTTGGCAAAGCCTGAAACTGATAAAGAACAGGAC AGTGAAATGGAGAAGGGTGGAAGAGAAGATATGGATATAAGTAAATCTGCAGAGGAGCCACAGGAAAAAGTTGACTTGACTCTAGATTGGTTAACTGAAA CCTCTGAAGAGGCAAAAGGAGGAGCAGCACCAGAAGGACCGAATGAAGCTGAGGTCACTTCTGGGAAGCCAGAACAGGAAGTACCAGATGCTGAGGAAGA AAAATCAGTTTCTGGAACTGATGTCCAAGAAGAGTGCAGAGAAAAAGGAGGTCAGGAGAAGCAGGGAGAGGTAATTGTGAGCATAGAGGAGAAGCCAAAA GAAGTTTCAGAAGAGCAGCCTGTGGTGACTCTAGAAAAGCAGGGCACTGCAGTGGAGGTAGAAGCAGAGTCTTTAGACCCGACAGTCAAGCCAGTGGATG TGGGTGGGGACGAGCCAGAGGAGAAGGTAGTTACCTCTGAAAACGAGGCAGGAAAGGCGGTTCTTGAACAACTGGTAGGTCAAGAAGTACCACCTGCTGA AGAGTCACCAGAGGTGACAACAGAGGCTGCAGAGGCCTCAGCTGTAGAGGCTGGATCAGAAGTCTCTGAAAAGCCTGGGCAGGAGGCTCCAGTTCTCCCT AAGGATGGTGCAGTCAATGGACCGTCAGTTGTAGGAGATCAGACTCCTATTGAACCACAGACTTCTATAGAAAGACTGACAGAAACAAAAGATGGCTCAG GACTAGAGGAGAAGGTCAGGGCAAAGCTGGTTCCTAGTCAGGAGGAGACTAAGCTGTCTGTAGAAGAGTCTGAGGCAGCTGGAGATGGGGTTGATACCAA GGTAGCCCAGGGAGCTACTGAGAAATCACCTGAAGACAAAGTTCAGATAGCTGCTAATGAAGAGACACAAGAGAGAGAAGAACAGATGAAAGAGGGTGAA GAAACTGAAGGCTCAGAAGAGGATGATAAAGAAAATGATAAGACCGAAGAAATGCCAAATGATTCAGTCCTTGAAAACAAGTCTCTTCAAGAAAATGAGG AGGAGGAGATTGGGAACCTAGAGCTTGCCTGGGATATGCTGGATTTAGCAAAGATCATTTTTAAAAGGCAAGAAACAAAAGAAGCACAGCTTTATGCTGC CCAGGCACATCTTAAACTCGGAGAAGTTAGTGTTGAATCTGAAAACTATGTGCAAGCTGTGGAGGAGTTCCAGTCCTGCCTTAACCTGCAGGAACAGTAC CTGGAAGCCCACGACCGTCTCCTTGCAGAGACCCACTACCAGCTGGGCTTGGCTTATGGGTACAACTCTCAGTATGATGAGGCAGTGGCACAGTTCAGCA AATCTATTGAAGTCATTGAGAACAGAATGGCTGTACTAAACGAGCAGGTGAAGGAGGCTGAAGGATCGTCTGCTGAATACAAGAAAGAAATTGAGGAACT AAAGGAACTGCTACCCGAAATTAGAGAGAAGATAGAAGATGCAAAGGAGTCTCAGCGTAGTGGGAATGTAGCTGAACTGGCTCTGAAAGCTACTCTGGTG GAGAGTTCTACTTCAGGTTTCACTCCTGGTGGAGGAGGCTCTTCAGTCTCCATGATTGCCAGTAGAAAGCCAACAGACGGTGCTTCCTCATCAAATTGTG TGACTGATATTTCCCACCTTGTCAGAAAGAAGAGGAAACCAGAGGAAGAGAGTCCCCGGAAAGATGATGCAAAGAAAGCCAAACAAGAGCCGGAGGTGAA CGGAGGCAGTGGGGATGCTGTCCCCAGTGGAAATGAAGTTTCGGAAAACATGGAGGAGGAGGCTGAGAATCAGGCTGAAAGCCGGGCAGCAGTGGAGGGG ACAGTGGAGGCTGGAGCTACAGTTGAAAGCACTGCATGTTAAGAGGGGGCACAGCCCTCCTCCCAAGGGAAAGTGTTTTTGTATATAATGTATTTTTTCA CTTTTGGAGGATTCTTTTTGTATAACTTCAATAAAGATTGTAAGCAAAGGTTGAGGCTTTGATGGTTTTTTTCTTAATTATTGGCTGAATCTGCCTTGGA GCACTGCTGGTTTTATATATTAGCCAAAGGTTTTGTTCTGGCCTTCTGTACTGATCTGTGTTCCTGATCCTAATTCCTATCTGTCTAACGTGGAGGTGAT CAAGTGTGGCTGTAGGCCTTTGTTTTCCAATGGTGCTATATTCTGTTTTCAAACACTTCACTGAACCCAGCTGTCTTGCAAACTTTCAGTGGTGCTGTCC CTGGATGGGGGCTACAAAAACAAGAATTGGTGAAGATCTTGCTCTTCAGTGCTGAAAATGGATGATGGACTTTGGCTGTGAGCCAGGCCTAGGATGGTTC TTGTCCTATATCCACCTAGTCTTCACCTGGGGCTATAATTCTGTCCTGGAAAAAGAACTCTGAAAACCTGGGTCAGGGGAATGATTCCTAAGGAAAACGG TCTGCATTTGAGCTCTGGTTTGAAAGTAGCCAAGGGGACTGATGGTGGACACTCCAGATGTGGTTGGAAGCATATGTGGGGAGGCTGGCTGGTTGAGTTT TGTTATTTTCTGTATAGAAAGGTTGAGATATATCAACACTTGGAATTGTTACCCATCTGCAGAATTGACTTCTCAAATAAAGATGCTAAAAATCTC

refseq_NASP.F1 (forward primer)
refseq_NASP.R1 (reverse primer)
Single exon skipping

Frame 0

NLPFSVPE*VSGVPNCLFFSQALFRSLVRHLRG TMAMESTATAAVAAELVSADKIEDVPAPSTSADK VESNH*SAFCH*RWVAFCRILSK*NHTSGCG** S*ETIGFRTETSGDGGYSSSCQCIPGSS*SFR* EVWRDS**VWRSLLFLWEITSGVGKNGEWCVGKR LGRCACGRGRRRKNRR*ISGRK***HR*GSKGR VERTGL*RHGRKRRSQKNRRQVFGKA*N**RTG Q*NGEGWKRRYGYK*ICRGATGKS*LDSRLVN*N L*RGKRRSSTRRTE*S*GHFWEARTGSTRC*GR KISFWN*CPRRVQRKRRSGEAGRGNCEHRGEAK RSFRRAACGDSRKAGHCSGGRSRVFRPDSQASGC GWGRARGEGSYL*KRGRKGGS*TTGRSRSTTC* RVTRGDNRGCRGLSCRGWIRSL*KAWAGGSSSP *GWCSQWTVSCRRSDSY*TTDFYRKTDRNKRWLR TRGEGQGKAGS*SGGD*AVCRRV*GSWRWG*YQ GSPGSY*EIT*RQSSDSC**RDTRERRTDERG* RN*RLRRG**RK**DRRNAK*FSP*KQVSSRK*G GGDWEPRACLGYAGFSKDHF*KARNKRSTALCC PGTS*TRRS*C*I*KLCASCGGVPVLP*PAGTV PGSPRPSPCRDPLPAGLGLWVQLSV**GSGTVQQ IY*SH*EQNGCTKRAGEGG*RIVC*IQERN*GT KGTATRN*REDRRCKGVSA*WECS*TGSESYSG GEFYFRFHSWWRRLFSLHDCQ*KANRRCFLIKLC D*YFPPCQKEEETRGRESPER*CKESQTRAGGE RRQWGCCPQWK*SFGKHGGGG*ESG*KPGSSGG DSGGWSYS*KHCMLRGGTALLPRESVFVYNVFFH FWRILFV*LQ*RL*AKVEALMVFFLIIG*ICLG ALLVLYISQRFCSGLLY*SVFLILIPICLTWR* SSVAVGLCFPMVLYSVFKHFTEPSCLANFQWCCP WMGATKTRIGEDLALQC*KWMMDFGCEPGLGWF LSYIHLVFTWGYNSVLEKEL*KPGSGE*FLRKT VCI*ALV*K*PRGLMVDTPDVVGSICGEAGWLSF VIFCIERLRYINTWNCYPSAELTSQIKMLKI

Frame 1

ICHFLSLSESLASQIACFSRRLYSVRWFATSGE RWPWSPQPLPPSPRSWFLPTKLKMFLLLLHLQI KWRATINLLSVTEDGLHFVEYYLNRIIHLDVDSE AKKLLGLGQKHLVMGDIPAAVNAFQEAASLLGK KYGETANECGEAFFFYGKSLLELARMENGVLGN ALEGVHVEEEEGEKTEDESLVENNDNIDEEAREE LREQVYDAMGEKEEAKKTEDKSLAKPETDKEQD SEMEKGGREDMDISKSAEEPQEKVDLTLDWLTE TSEEAKGGAAPEGPNEAEVTSGKPEQEVPDAEEE KSVSGTDVQEECREKGGQEKQGEVIVSIEEKPK EVSEEQPVVTLEKQGTAVEVEAESLDPTVKPVD VGGDEPEEKVVTSENEAGKAVLEQLVGQEVPPAE ESPEVTTEAAEASAVEAGSEVSEKPGQEAPVLP KDGAVNGPSVVGDQTPIEPQTSIERLTETKDGS GLEEKVRAKLVPSQEETKLSVEESEAAGDGVDTK VAQGATEKSPEDKVQIAANEETQEREEQMKEGE ETEGSEEDDKENDKTEEMPNDSVLENKSLQENE EEEIGNLELAWDMLDLAKIIFKRQETKEAQLYAA QAHLKLGEVSVESENYVQAVEEFQSCLNLQEQY LEAHDRLLAETHYQLGLAYGYNSQYDEAVAQFS KSIEVIENRMAVLNEQVKEAEGSSAEYKKEIEEL KELLPEIREKIEDAKESQRSGNVAELALKATLV ESSTSGFTPGGGGSSVSMIASRKPTDGASSSNC VTDISHLVRKKRKPEEESPRKDDAKKAKQEPEVN GGSGDAVPSGNEVSENMEEEAENQAESRAAVEG TVEAGATVESTAC*EGAQPSSQGKVFLYIMYFF TFGGFFLYNFNKDCKQRLRL*WFFS*LLAESALE HCWFYILAKGFVLAFCTDLCS*S*FLSV*RGGD QVWL*AFVFQWCYILFSNTSLNPAVLQTFSGAV PGWGLQKQELVKILLFSAENG*WTLAVSQA*DGS CPIST*SSPGAIILSWKKNSENLGQGNDS*GKR SAFELWFESSQGD*WWTLQMWLEAYVGRLAG*V LLFSV*KG*DISTLGIVTHLQN*LLK*RC*KS

Frame 2

SAIFCP*VSLWRPKLPVFLAGSIPFAGSPPQGN DGHGVHSHCRRRRGAGFCRQN*RCSCSFYICR* SGEQPLICFLSLKMGCIL*NII*IESYIWMWIV KLRNYWV*DRNIW*WGIFQQLSMHSRKQLVF*VR SMERQLMSVEKPSFSMGNHFWSWQEWRMVCWET PWKVCMWKRKKEKKQKMNLW*KIMIT*MRKQGK S*ENRFMTPWEKKKKPKKQKTSLWQSLKLIKNRT VKWRRVEEKIWI*VNLQRSHRKKLT*L*IG*LK PLKRQKEEQHQKDRMKLRSLLGSQNRKYQMLRK KNQFLELMSKKSAEKKEVRRSRER*L*A*RRSQK KFQKSSLW*L*KSRALQWR*KQSL*TRQSSQWM WVGTSQRRR*LPLKTRQERRFLNNW*VKKYHLL KSHQR*QQRLQRPQL*RLDQKSLKSLGRRLQFSL RMVQSMDRQL*EIRLLLNHRLL*KD*QKQKMAQ D*RRRSGQSWFLVRRRLSCL*KSLRQLEMGLIP R*PRELLRNHLKTKFR*LLMKRHKREKNR*KRVK KLKAQKRMIKKMIRPKKCQMIQSLKTSLFKKMR RRRLGT*SLPGICWI*QRSFLKGKKQKKHSFML PRHILNSEKLVLNLKTMCKLWRSSSPALTCRNST WKPTTVSLQRPTTSWAWLMGTTLSMMRQWHSSA NLLKSLRTEWLY*TSR*RRLKDRLLNTRKKLRN *RNCYPKLERR*KMQRSLSVVGM*LNWL*KLLWW RVLLQVSLLVEEALQSP*LPVESQQTVLPHQIV *LIFPTLSERRGNQRKRVPGKMMQRKPNKSRR* TEAVGMLSPVEMKFRKTWRRRLRIRLKAGQQWRG QWRLELQLKALHVKRGHSPPPKGKCFCI*CIFS LLEDSFCITSIKIVSKG*GFDGFFLNYWLNLPW STAGFIY*PKVLFWPSVLICVPDPNSYLSNVEVI KCGCRPLFSNGAIFCFQTLH*TQLSCKLSVVLS LDGGYKNKNW*RSCSSVLKMDDGLWL*ARPRMV LVLYPPSLHLGL*FCPGKRTLKTWVRGMIPKENG LHLSSGLKVAKGTDGGHSRCGWKHMWGGWLVEF CYFLYRKVEIYQHLELLPICRIDFSNKDAKNL