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Sequence NM_023033
CCGAGACCAGGAGTAAGGGGACAGGAGCGAGGACTGATGCCACTGTAGAAGGCGAGGTCTCTAGACCCCTCTCACCTGTGGGGTGCACAGGTGCCTGCAACCAGGATGGTAACCCAGACCAGGCAGAAGCAAGCTAAGAGAAAACGTTGGGTCCCTGGGCCTGGCCTCGCCCTCCCAGTTGGAGGCTAACGGTGCGGAGG
TTCCCTCCAGCACTCGCTCCTTACAGCCCTCCCCACCTAGCCCAGCCCAGCCCAGCCCAGCCCAGCTGAATTTGCTCTTGGGTCTTTAAGGGGCGGGAAC
TGCCACCAACGCGCATGGCTGCGTCATTAACTTGCGCCTCTGGCCTGCGCCTCCACGTGGATTTGGTAGAGAAACCCCGGACTGGGATCATGGCAGCCGA
GACTCGGAACGTGGCCGGAGCAGAGGCCCCACCGCCCCAGAAGCGCTACTACCGGCAACGTGCTCACTCCAACCCCATGGCGGACCACACGCTGCGCTAC
CCTGTGAAGCCAGAGGAGATGGACTGGTCTGAGCTATACCCAGAGTTCTTCGCTCCACTCACTCAAAATCAGAGCCACGATGACCCAAAGGATAAGAAAG
AAAAGAGAGCTCAGGCCCAAGTGGAGTTTGCAGACATAGGCTGTGGCTATGGTGGCCTGTTAGCTGACAAAGATGTTCTTCCTCTTCCCCGACCCACATT
TCAAGCGGACAAAGCACAAGTGGCGAATCATCAGTCCCACCCTGCTAGCAGAATATGCCTACGTGCTAAGAGTTGGGGGGCTGGTGTATACCATAACCGA
TGTGCTGGAGCTACACGACTGGATGTGCACTCATTTCGAAGAGCACCCACTGTTTGAGCGTGTGCCTCTGGAGGACCTGAGTGAAGACCCCGTTGTGGGA
CATCTAGGCACCTCAACTGAGGAGGGGAAGAAAGTTCTACGTAATGGAGGGAAGAATTTCCCAGCCATCTTCCGAAGAATACAAGATCCCGTCCTCCAGG
CAGTGACCTCCCAAACCAGCCTGCCTGGTCACTGACTGCTTACTCTACCTTAGCTGGACCTCGTCTCCCAGGGATTAGAGAAAAGAGCAGGAGTCCTGGG
TCTTCCCAGTTGAGACTGCTGGAGCTGAGACACAGTACTCTCTTAAAGAAGGTGGGGAGCTGCCCAGGGCAGAACCCACTGGTGTTCATGACTACCCCTG
GCTCCTCTCACCTTGTCCCTCCACTGCCAACAGAAAACAAAGCAGCTGACTGAGATGGTCAAAGGACTTTGGACCATAGGGGATCTTTGGAAGGCTGTGG
GGTCTTGCTCTTCCTTAGCACTCCTTTCTCCTTGTGAGATCTCTCCTCAGCTGGAATGAGGAATGTAGTCCATCTAAACTGCTTGCTAGGCTCAATTACC
ACTTCTGTTTGCTTTGTGGATCCTGGGATAACATGTATATGTGTACACATGCCTATTCTGCCATTTCTCAGGAAAGGGTGGAGTATGTATCATATGCCTT
CTACCATCCTCCTGTCCTCTCCCTGCGTAAGACTTGACCTGGGGAATCTGTGTTCCCTAA
- refseq_METTL1.F1 (forward primer)
- refseq_METTL1.R1 (reverse primer)
- Single exon skipping
Frame 0
PRPGVRGQERGLMPL*KARSLDPSHLWGAQVPATRMVTQTRQKQAKRKRWVPGPGLALPVGG*RCGG
SLQHSLLTALPT*PSPAQPSPAEFALGSLRGGN
CHQRAWLRH*LAPLACASTWIW*RNPGLGSWQP
RLGTWPEQRPHRPRSATTGNVLTPTPWRTTRCAT
L*SQRRWTGLSYTQSSSLHSLKIRATMTQRIRK
KRELRPKWSLQT*AVAMVAC*LTKMFFLFPDPH
FKRTKHKWRIISPTLLAEYAYVLRVGGLVYTITD
VLELHDWMCTHFEEHPLFERVPLEDLSEDPVVG
HLGTSTEEGKKVLRNGGKNFPAIFRRIQDPVLQ
AVTSQTSLPGH*LLTLP*LDLVSQGLEKRAGVLG
LPS*DCWS*DTVLS*RRWGAAQGRTHWCS*LPL
APLTLSLHCQQKTKQLTEMVKGLWTIGDLWKAV
GSCSSLALLSPCEISPQLE*GM*SI*TAC*AQLP
LLFALWILG*HVYVYTCLFCHFSGKGGVCIICL
LPSSCPLPA*DLTWGICVP*
Frame 1
RDQE*GDRSED*CHCRRRGL*TPLTCGVHRCLQPGW*PRPGRSKLRENVGSLGLASPSQLEANGAE
VPSSTRSLQPSPPSPAQPSPAQLNLLLGL*GAGT
ATNAHGCVINLRLWPAPPRGFGRETPDWDHGSR
DSERGRSRGPTAPEALLPATCSLQPHGGPHAAL
PCEARGDGLV*AIPRVLRSTHSKSEPR*PKG*ER
KESSGPSGVCRHRLWLWWPVS*QRCSSSSPTHI
SSGQSTSGESSVPPC*QNMPTC*ELGGWCIP*P
MCWSYTTGCALISKSTHCLSVCLWRT*VKTPLWD
I*APQLRRGRKFYVMEGRISQPSSEEYKIPSSR
Q*PPKPACLVTDCLLYLSWTSSPRD*RKEQESW
VFPVETAGAETQYSLKEGGELPRAEPTGVHDYPW
LLSPCPSTANRKQSS*LRWSKDFGP*GIFGRLW
GLALP*HSFLLVRSLLSWNEECSPSKLLARLNY
HFCLLCGSWDNMYMCTHAYSAISQERVEYVSYAF
YHPPVLSLRKT*PGESVFP
Frame 2
ETRSKGTGARTDATVEGEVSRPLSPVGCTGACNQDGNPDQAEAS*EKTLGPWAWPRPPSWRLTVRR
FPPALAPYSPPHLAQPSPAQPS*ICSWVFKGRE
LPPTRMAASLTCASGLRLHVDLVEKPRTGIMAAE
TRNVAGAEAPPPQKRYYRQRAHSNPMADHTLRY
PVKPEEMDWSELYPEFFAPLTQNQSHDDPKDKK
EKRAQAQVEFADIGCGYGGLLADKDVLPLPRPTF
QADKAQVANHQSHPASRICLRAKSWGAGVYHNR
CAGATRLDVHSFRRAPTV*ACASGGPE*RPRCG
TSRHLN*GGEESST*WREEFPSHLPKNTRSRPPG
SDLPNQPAWSLTAYSTLAGPRLPGIREKSRSPG
SSQLRLLELRHSTLLKKVGSCPGQNPLVFMTTP
GSSHLVPPLPTENKAAD*DGQRTLDHRGSLEGCG
VLLFLSTPFSL*DLSSAGMRNVVHLNCLLGSIT
TSVCFVDPGITCICVHMPILPFLRKGWSMYHMP
STILLSSPCVRLDLGNLCSL
Sequence NM_005371
CCGAGACCAGGAGTAAGGGGACAGGAGCGAGGACTGATGCCACTGTAGAAGGCGAGGTCTCTAGACCCCTCTCACCTGTGGGGTGCACAGGTGCCTGCAACCAGGATGGTAACCCAGACCAGGCAGAAGCAAGCTAAGAGAAAACGTTGGGTCCCTGGGCCTGGCCTCGCCCTCCCAGTTGGAGGCTAACGGTGCGGAGG
TTCCCTCCAGCACTCGCTCCTTACAGCCCTCCCCACCTAGCCCAGCCCAGCCCAGCCCAGCCCAGCTGAATTTGCTCTTGGGTCTTTAAGGGGCGGGAAC
TGCCACCAACGCGCATGGCTGCGTCATTAACTTGCGCCTCTGGCCTGCGCCTCCACGTGGATTTGGTAGAGAAACCCCGGACTGGGATCATGGCAGCCGA
GACTCGGAACGTGGCCGGAGCAGAGGCCCCACCGCCCCAGAAGCGCTACTACCGGCAACGTGCTCACTCCAACCCCATGGCGGACCACACGCTGCGCTAC
CCTGTGAAGCCAGAGGAGATGGACTGGTCTGAGCTATACCCAGAGTTCTTCGCTCCACTCACTCAAAATCAGAGCCACGATGACCCAAAGGATAAGAAAG
AAAAGAGAGCTCAGGCCCAAGTGGAGTTTGCAGACATAGGCTGTGGCTATGGTGGCCTGTTAGTGGAACTGTCACCGCTGTTCCCAGACACACTTATTCT
GGGTCTGGAGATCCGGGTGAAGGTCTCAGACTATGTACAAGACCGGATTCGGGCCCTACGCGCAGCTCCTGCAGGTGGCTTCCAGAACATCGCCTGTCTC
CGTAGCAATGCCATGAAGCACCTTCCTAACTTCTTCTACAAGGGCCAGCTGACAAAGATGTTCTTCCTCTTCCCCGACCCACATTTCAAGCGGACAAAGC
ACAAGTGGCGAATCATCAGTCCCACCCTGCTAGCAGAATATGCCTACGTGCTAAGAGTTGGGGGGCTGGTGTATACCATAACCGATGTGCTGGAGCTACA
CGACTGGATGTGCACTCATTTCGAAGAGCACCCACTGTTTGAGCGTGTGCCTCTGGAGGACCTGAGTGAAGACCCCGTTGTGGGACATCTAGGCACCTCA
ACTGAGGAGGGGAAGAAAGTTCTACGTAATGGAGGGAAGAATTTCCCAGCCATCTTCCGAAGAATACAAGATCCCGTCCTCCAGGCAGTGACCTCCCAAA
CCAGCCTGCCTGGTCACTGACTGCTTACTCTACCTTAGCTGGACCTCGTCTCCCAGGGATTAGAGAAAAGAGCAGGAGTCCTGGGTCTTCCCAGTTGAGA
CTGCTGGAGCTGAGACACAGTACTCTCTTAAAGAAGGTGGGGAGCTGCCCAGGGCAGAACCCACTGGTGTTCATGACTACCCCTGGCTCCTCTCACCTTG
TCCCTCCACTGCCAACAGAAAACAAAGCAGCTGACTGAGATGGTCAAAGGACTTTGGACCATAGGGGATCTTTGGAAGGCTGTGGGGTCTTGCTCTTCCT
TAGCACTCCTTTCTCCTTGTGAGATCTCTCCTCAGCTGGAATGAGGAATGTAGTCCATCTAAACTGCTTGCTAGGCTCAATTACCACTTCTGTTTGCTTT
GTGGATCCTGGGATAACATGTATATGTGTACACATGCCTATTCTGCCATTTCTCAGGAAAGGGTGGAGTATGTATCATATGCCTTCTACCATCCTCCTGT
CCTCTCCCTGCGTAAGACTTGACCTGGGGAATCTGTGTTCCCTAA
- refseq_METTL1.F1 (forward primer)
- refseq_METTL1.R1 (reverse primer)
- Single exon skipping
Frame 0
PRPGVRGQERGLMPL*KARSLDPSHLWGAQVPATRMVTQTRQKQAKRKRWVPGPGLALPVGG*RCGG
SLQHSLLTALPT*PSPAQPSPAEFALGSLRGGN
CHQRAWLRH*LAPLACASTWIW*RNPGLGSWQP
RLGTWPEQRPHRPRSATTGNVLTPTPWRTTRCAT
L*SQRRWTGLSYTQSSSLHSLKIRATMTQRIRK
KRELRPKWSLQT*AVAMVAC*WNCHRCSQTHLF
WVWRSG*RSQTMYKTGFGPYAQLLQVASRTSPVS
VAMP*STFLTSSTRAS*QRCSSSSPTHISSGQS
TSGESSVPPC*QNMPTC*ELGGWCIP*PMCWSY
TTGCALISKSTHCLSVCLWRT*VKTPLWDI*APQ
LRRGRKFYVMEGRISQPSSEEYKIPSSRQ*PPK
PACLVTDCLLYLSWTSSPRD*RKEQESWVFPVE
TAGAETQYSLKEGGELPRAEPTGVHDYPWLLSPC
PSTANRKQSS*LRWSKDFGP*GIFGRLWGLALP
*HSFLLVRSLLSWNEECSPSKLLARLNYHFCLL
CGSWDNMYMCTHAYSAISQERVEYVSYAFYHPPV
LSLRKT*PGESVFP
Frame 1
RDQE*GDRSED*CHCRRRGL*TPLTCGVHRCLQPGW*PRPGRSKLRENVGSLGLASPSQLEANGAE
VPSSTRSLQPSPPSPAQPSPAQLNLLLGL*GAGT
ATNAHGCVINLRLWPAPPRGFGRETPDWDHGSR
DSERGRSRGPTAPEALLPATCSLQPHGGPHAAL
PCEARGDGLV*AIPRVLRSTHSKSEPR*PKG*ER
KESSGPSGVCRHRLWLWWPVSGTVTAVPRHTYS
GSGDPGEGLRLCTRPDSGPTRSSCRWLPEHRLS
P*QCHEAPS*LLLQGPADKDVLPLPRPTFQADKA
QVANHQSHPASRICLRAKSWGAGVYHNRCAGAT
RLDVHSFRRAPTV*ACASGGPE*RPRCGTSRHL
N*GGEESST*WREEFPSHLPKNTRSRPPGSDLPN
QPAWSLTAYSTLAGPRLPGIREKSRSPGSSQLR
LLELRHSTLLKKVGSCPGQNPLVFMTTPGSSHL
VPPLPTENKAAD*DGQRTLDHRGSLEGCGVLLFL
STPFSL*DLSSAGMRNVVHLNCLLGSITTSVCF
VDPGITCICVHMPILPFLRKGWSMYHMPSTILL
SSPCVRLDLGNLCSL
Frame 2
ETRSKGTGARTDATVEGEVSRPLSPVGCTGACNQDGNPDQAEAS*EKTLGPWAWPRPPSWRLTVRR
FPPALAPYSPPHLAQPSPAQPS*ICSWVFKGRE
LPPTRMAASLTCASGLRLHVDLVEKPRTGIMAAE
TRNVAGAEAPPPQKRYYRQRAHSNPMADHTLRY
PVKPEEMDWSELYPEFFAPLTQNQSHDDPKDKK
EKRAQAQVEFADIGCGYGGLLVELSPLFPDTLIL
GLEIRVKVSDYVQDRIRALRAAPAGGFQNIACL
RSNAMKHLPNFFYKGQLTKMFFLFPDPHFKRTK
HKWRIISPTLLAEYAYVLRVGGLVYTITDVLELH
DWMCTHFEEHPLFERVPLEDLSEDPVVGHLGTS
TEEGKKVLRNGGKNFPAIFRRIQDPVLQAVTSQ
TSLPGH*LLTLP*LDLVSQGLEKRAGVLGLPS*D
CWS*DTVLS*RRWGAAQGRTHWCS*LPLAPLTL
SLHCQQKTKQLTEMVKGLWTIGDLWKAVGSCSS
LALLSPCEISPQLE*GM*SI*TAC*AQLPLLFAL
WILG*HVYVYTCLFCHFSGKGGVCIICLLPSSC
PLPA*DLTWGICVP*
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