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Sequences

Sequence NM_005355

TTGTTTACAAAAACAGGCGGCAGGCTGGGGTTGGTGGGGCTTGTGTTTAATTAACGCAGCACACAAAGTCTTATGACGTCGTTTTACTCTTTTCTCTCAG
GAAACAATTCTGGTGAAATGTGATCGTCCTGGCCACCCTCTGATTGATAAGACATATCATTTTGAAAGATATACTGGCCTTCCCAGCTGACATGGACCTC
AGGTCAGCTTCAGCGTGAGAAGCAGGCCAGGCCTGGGTCTGGAGCCGTCCTGGCCTTCCCAGATGACATGGACCTCAGGTCAGCTTCAGCGTGAGAAGCA
GGCCAGGCCTGGGTCTGGAGCCGTCCTGGCCTTCCCAGATGACAAGGACCTCAGGGTTTATGGTCCAGCAGAGTCTCAGAGCGCGGTCTTTGGAGATGTG
TGCCCCCTACTCACTTCTCTCTTGGATGGGTACAATGTTTGTGTTATGGCGTATGGACAGACGGGCAGCGGAAAGAGCTATACCATGCTGGGACGCCATT
CGGACGACGGCCCTGTTCTGCCGCTTGACCCACAGAGTGACTTAGGAATTATCCCTAGAGTGGCTGAGGAGCTCTTCAGGCTCATTTTGGAAAATACCTC
AAGAAGCCCAAAGGTTGAAGTCTCCATAGTGGAAGTTTACAATAATGACATTTTTGACCTTCTGGCCAAAGACAGCATTGCAGCAGTGTCGGGGGTCAAG
CGTGAGGTGGTGACAGCCAAGGATGGACGGACAGAGGTTGCGCTGCTGGCCTCTGAGGCTGTCGGCAGCGCCTCGAAACTGATGGAGCTCGTTCATGGAG
GTCTGCAGCTCAGGGCGAAGCACCCCACCCTGGTGCACGCGGATTCCTCCAGGTCTCACCTGATAATTACGGTGACTCTAACCACAGCCTCCTGCTCTGA
CAGCACTGCAGACCAAGCCTGCAGTGCCACCCTCCCCAGGGAGCAAACAGAGGCAGGAAGGGCAGGAAGGAGCCGCAGAGCTTCTCAAGGGGCCTTGGCT
CCACAGCTGGTTCCTGGGAACCCCGCAGGGCATGCGGAGCAGGTGCAGGCTCGACTACAGCTCGTGGACTCGGCCGGCAGCGAGTGCGTTGGAGGCGATG
CGAAGTTACTGGTGA
TTCTCTGCATTTCTCCCAGCCAGAGGCACCTGGCACAGACGTTGCAGGGCCTGGGTTTCGGGATCCGAGCTCGGCAAGTCCAGCG
AGGCCCTGCCCGAAAGAAGCCGCCCAGCTCCCAAACGGAGGGGAAGAGGAGGCCGGATTGAATGCATTAACAAGTTTTTCTCCTAAAACTGTGTTTCTTG
TCCTTGCTTTATAATGCATATGTGCTTAGAAATAAACAGGTTTCACGTGGACTC
  • refseq_KIF25.F1 (forward primer)
  • refseq_KIF25.R1 (reverse primer)
  • Single exon skipping

Frame 0

LFTKTGGRLGLVGLVFN*RSTQSLMTSFYSFLS
GNNSGEM*SSWPPSD**DISF*KIYWPSQLTWTS
GQLQREKQARPGSGAVLAFPDDMDLRSASA*EA
GQAWVWSRPGLPR*QGPQGLWSSRVSERGLWRC
VPPTHFSLGWVQCLCYGVWTDGQRKELYHAGTPF
GRRPCSAA*PTE*LRNYP*SG*GALQAHFGKYL
KKPKG*SLHSGSLQ**HF*PSGQRQHCSSVGGQ
A*GGDSQGWTDRGCAAGL*GCRQRLETDGARSWR
SAAQGEAPHPGARGFLQVSPDNYGDSNHSLLL*
QHCRPSLQCHPPQGANRGRKGRKEPQSFSRGLG
STAGSWEPRRACGAGAGSTTARGLGRQRVRWRRC
EVTGD
SLHFSQPEAPGTDVAGPGFRDPSSASPA
RPCPKEAAQLPNGGEEEAGLNALTSFSPKTVFL
VLAL*CICA*K*TGFTWT

Frame 1

CLQKQAAGWGWWGLCLINAAHKVL*RRFTLFSQ
ETILVKCDRPGHPLIDKTYHFERYTGLPS*HGP
QVSFSVRSRPGLGLEPSWPSQMTWTSGQLQREKQ
ARPGSGAVLAFPDDKDLRVYGPAESQSAVFGDV
CPLLTSLLDGYNVCVMAYGQTGSGKSYTMLGRH
SDDGPVLPLDPQSDLGIIPRVAEELFRLILENTS
RSPKVEVSIVEVYNNDIFDLLAKDSIAAVSGVK
REVVTAKDGRTEVALLASEAVGSASKLMELVHG
GLQLRAKHPTLVHADSSRSHLIITVTLTTASCSD
STADQACSATLPREQTEAGRAGRSRRASQGALA
PQLVPGNPAGHAEQVQARLQLVDSAGSECVGGD
AKLLV
ILCISPSQRHLAQTLQGLGFGIRARQVQR
GPARKKPPSSQTEGKRRPD*MH*QVFLLKLCFL
SLLYNAYVLRNKQVSRGL

Frame 2

VYKNRRQAGVGGACV*LTQHTKSYDVVLLFSLR
KQFW*NVIVLATL*LIRHIILKDILAFPADMDL
RSASA*EAGQAWVWSRPGLPR*HGPQVSFSVRS
RPGLGLEPSWPSQMTRTSGFMVQQSLRARSLEMC
APYSLLSWMGTMFVLWRMDRRAAERAIPCWDAI
RTTALFCRLTHRVT*ELSLEWLRSSSGSFWKIP
QEAQRLKSP*WKFTIMTFLTFWPKTALQQCRGSS
VRW*QPRMDGQRLRCWPLRLSAAPRN*WSSFME
VCSSGRSTPPWCTRIPPGLT**LR*L*PQPPAL
TALQTKPAVPPSPGSKQRQEGQEGAAELLKGPWL
HSWFLGTPQGMRSRCRLDYSSWTRPAASALEAM
RSYW*
FSAFLPARGTWHRRCRAWVSGSELGKSS
EALPERSRPAPKRRGRGGRIECINKFFS*NCVSC
PCFIMHMCLEINRFHVD

Sequence NM_030615

TTGTTTACAAAAACAGGCGGCAGGCTGGGGTTGGTGGGGCTTGTGTTTAATTAACGCAGCACACAAAGTCTTATGACGTCGTTTTACTCTTTTCTCTCAG
GAAACAATTCTGGTGAAATGTGATCGTCCTGGCCACCCTCTGATTGATAAGACATATCATTTTGAAAGATATACTGGCCTTCCCAGCTGACATGGACCTC
AGGTCAGCTTCAGCGTGAGAAGCAGGCCAGGCCTGGGTCTGGAGCCGTCCTGGCCTTCCCAGATGACATGGACCTCAGGTCAGCTTCAGCGTGAGAAGCA
GGCCAGGCCTGGGTCTGGAGCCGTCCTGGCCTTCCCAGATGACAAGGACCTCAGGGTTTATGGTCCAGCAGAGTCTCAGAGCGCGGTCTTTGGAGATGTG
TGCCCCCTACTCACTTCTCTCTTGGATGGGTACAATGTTTGTGTTATGGCGTATGGACAGACGGGCAGCGGAAAGAGCTATACCATGCTGGGACGCCATT
CGGACGACGGCCCTGTTCTGCCGCTTGACCCACAGAGTGACTTAGGAATTATCCCTAGAGTGGCTGAGGAGCTCTTCAGGCTCATTTTGGAAAATACCTC
AAGAAGCCCAAAGGTTGAAGTCTCCATAGTGGAAGTTTACAATAATGACATTTTTGACCTTCTGGCCAAAGACAGCATTGCAGCAGTGTCGGGGGTCAAG
CGTGAGGTGGTGACAGCCAAGGATGGACGGACAGAGGTTGCGCTGCTGGCCTCTGAGGCTGTCGGCAGCGCCTCGAAACTGATGGAGCTCGTTCATGGAG
GTCTGCAGCTCAGGGCGAAGCACCCCACCCTGGTGCACGCGGATTCCTCCAGGTCTCACCTGATAATTACGGTGACTCTAACCACAGCCTCCTGCTCTGA
CAGCACTGCAGACCAAGCCTGCAGTGCCACCCTCCCCAGGGAGCAAACAGAGGCAGGAAGGGCAGGAAGGAGCCGCAGAGCTTCTCAAGGGGCCTTGGCT
CCACAGCTGGTTCCTGGGAACCCCGCAGGGCATGCGGAGCAGGTGCAGGCTCGACTACAGCTCGTGGACTCGGCCGGCAGCGAGTGCGTTGGTGTGTCTG
GAGTGACCGGGTTGGCCCTGAGGGAGATGGCGTGCATCAGCCGCAGCCTTGCGGCCCTGGCAGGCGTCCTGGGGGCTTTGTTGGAGCACCGTGGCCATGC
CCCGTACCGGAACAGCAGGCTCACCCACCTCCTTCAGGACTGCCTCG
GAGGCGATGCGAAGTTACTGGTGATTCTCTGCATTTCTCCCAGCCAGAGGCAC
CTGGCACAGACGTTGCAGGGCCTGGGTTTCGGGATCCGAGCTCGGCAAGTCCAGCGAGGCCCTGCCCGAAAGAAGCCGCCCAGCTCCCAAACGGAGGGGA
AGAGGAGGCCGGATTGAATGCATTAACAAGTTTTTCTCCTAAAACTGTGTTTCTTGTCCTTGCTTTATAATGCATATGTGCTTAGAAATAAACAGGTTTC
ACGTGGACTC
  • refseq_KIF25.F1 (forward primer)
  • refseq_KIF25.R1 (reverse primer)
  • Single exon skipping

Frame 0

LFTKTGGRLGLVGLVFN*RSTQSLMTSFYSFLS
GNNSGEM*SSWPPSD**DISF*KIYWPSQLTWTS
GQLQREKQARPGSGAVLAFPDDMDLRSASA*EA
GQAWVWSRPGLPR*QGPQGLWSSRVSERGLWRC
VPPTHFSLGWVQCLCYGVWTDGQRKELYHAGTPF
GRRPCSAA*PTE*LRNYP*SG*GALQAHFGKYL
KKPKG*SLHSGSLQ**HF*PSGQRQHCSSVGGQ
A*GGDSQGWTDRGCAAGL*GCRQRLETDGARSWR
SAAQGEAPHPGARGFLQVSPDNYGDSNHSLLL*
QHCRPSLQCHPPQGANRGRKGRKEPQSFSRGLG
STAGSWEPRRACGAGAGSTTARGLGRQRVRWCVW
SDRVGPEGDGVHQPQPCGPGRRPGGFVGAPWPC
PVPEQQAHPPPSGLPR
RRCEVTGDSLHFSQPEA
PGTDVAGPGFRDPSSASPARPCPKEAAQLPNGGE
EEAGLNALTSFSPKTVFLVLAL*CICA*K*TGF
TWT

Frame 1

CLQKQAAGWGWWGLCLINAAHKVL*RRFTLFSQ
ETILVKCDRPGHPLIDKTYHFERYTGLPS*HGP
QVSFSVRSRPGLGLEPSWPSQMTWTSGQLQREKQ
ARPGSGAVLAFPDDKDLRVYGPAESQSAVFGDV
CPLLTSLLDGYNVCVMAYGQTGSGKSYTMLGRH
SDDGPVLPLDPQSDLGIIPRVAEELFRLILENTS
RSPKVEVSIVEVYNNDIFDLLAKDSIAAVSGVK
REVVTAKDGRTEVALLASEAVGSASKLMELVHG
GLQLRAKHPTLVHADSSRSHLIITVTLTTASCSD
STADQACSATLPREQTEAGRAGRSRRASQGALA
PQLVPGNPAGHAEQVQARLQLVDSAGSECVGVS
GVTGLALREMACISRSLAALAGVLGALLEHRGHA
PYRNSRLTHLLQDCL
GGDAKLLVILCISPSQRH
LAQTLQGLGFGIRARQVQRGPARKKPPSSQTEG
KRRPD*MH*QVFLLKLCFLSLLYNAYVLRNKQVS
RGL

Frame 2

VYKNRRQAGVGGACV*LTQHTKSYDVVLLFSLR
KQFW*NVIVLATL*LIRHIILKDILAFPADMDL
RSASA*EAGQAWVWSRPGLPR*HGPQVSFSVRS
RPGLGLEPSWPSQMTRTSGFMVQQSLRARSLEMC
APYSLLSWMGTMFVLWRMDRRAAERAIPCWDAI
RTTALFCRLTHRVT*ELSLEWLRSSSGSFWKIP
QEAQRLKSP*WKFTIMTFLTFWPKTALQQCRGSS
VRW*QPRMDGQRLRCWPLRLSAAPRN*WSSFME
VCSSGRSTPPWCTRIPPGLT**LR*L*PQPPAL
TALQTKPAVPPSPGSKQRQEGQEGAAELLKGPWL
HSWFLGTPQGMRSRCRLDYSSWTRPAASALVCL
E*PGWP*GRWRASAAALRPWQASWGLCWSTVAM
PRTGTAGSPTSFRTAS
EAMRSYW*FSAFLPARGT
WHRRCRAWVSGSELGKSSEALPERSRPAPKRRG
RGGRIECINKFFS*NCVSCPCFIMHMCLEINRF
HVD

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