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Sequences

Sequence NM_002474

GGGAGATTTGGACGCTCCGGCCTGGGAGGTGCGTCAGATCCGAGCTCGCCATCCAGTTTCCTCTCCACTAGTCCCCCCAGTTGGAGATCTGGGACCAACA AGGCACCATGGCGCAGAAGGGCCAACTCAGTGACGATGAGAAGTTCCTCTTTGTGGACAAAAACTTCATCAACAGCCCAGTGGCCCAGGCTGACTGGGCC GCCAAGAGACTCGTCTGGGTCCCCTCGGAGAAGCAGGGCTTCGAGGCAGCCAGCATTAAGGAGGAGAAGGGGGATGAGGTGGTTGTGGAGCTGGTGGAGA ATGGCAAGAAGGTCACGGTTGGGAAAGATGACATCCAGAAGATGAACCCACCCAAGTTCTCCAAGGTGGAGGACATGGCGGAGCTGACGTGCCTCAACGA AGCCTCCGTGCTACACAACCTGAGGGAGCGGTACTTCTCAGGGCTAATATATACGTACTCTGGCCTCTTCTGCGTGGTGGTCAACCCCTATAAACACCTG CCCATCTACTCGGAGAAGATCGTCGACATGTACAAGGGCAAGAAGAGGCACGAGATGCCGCCTCACATCTACGCCATCGCAGACACGGCCTACCGGAGCA TGCTTCAAGATCGGGAGGACCAGTCCATTCTATGCACAGGCGAGTCTGGAGCCGGGAAAACCGAAAACACCAAGAAGGTCATTCAGTACCTGGCCGTGGT GGCCTCCTCCCACAAGGGCAAGAAAGACACAAGTATCACGGGAGAGCTGGAAAAGCAGCTTCTACAAGCAAACCCGATTCTGGAGGCTTTCGGCAACGCC AAAACAGTGAAGAACGACAACTCCTCACGATTCGGCAAATTCATCCGCATCAACTTCGACGTCACGGGTTACATCGTGGGAGCCAACATTGAGACCTATC TGCTAGAAAAATCACGGGCAATTCGCCAAGCCAGAGACGAGAGGACATTCCACATCTTTTACTACATGATTGCTGGAGCCAAGGAGAAGATGAGAAGTGA CTTGCTTTTGGAGGGCTTCAACAACTACACCTTCCTCTCCAATGGCTTTGTGCCCATCCCAGCAGCCCAGGATGATGAGATGTTCCAGGAAACCGTGGAG GCCATGGCAATCATGGGTTTCAGCGAGGAGGAGCAGCTATCCATATTGAAGGTGGTATCATCGGTCCTGCAGCTTGGAAATATCGTCTTCAAGAAGGAAA GAAACACAGACCAGGCGTCCATGCCAGATAACACAGCTGCTCAGAAAGTTTGCCACCTCATGGGAATTAATGTGACAGATTTCACCAGATCCATCCTCAC TCCTCGTATCAAGGTTGGGCGAGATGTGGTACAGAAAGCTCAGACAAAAGAACAGGCTGACTTTGCTGTAGAGGCTTTGGCCAAGGCAACATATGAGCGC CTTTTCCGCTGGATACTCACCCGCGTGAACAAAGCCCTGGACAAGACCCATCGGCAAGGGGCTTCCTTCCTGGGGATCCTGGATATAGCTGGATTTGAGA TCTTTGAGGTGAACTCCTTCGAGCAGCTGTGCATCAACTACACCAACGAGAAGCTGCAGCAGCTCTTCAACCACACCATGTTCATCCTGGAGCAGGAGGA GTACCAGCGCGAGGGCATCGAGTGGAACTTCATCGACTTTGGGCTGGACCTACAGCCCTGCATCGAGCTCATCGAGCGACCGAACAACCCTCCAGGTGTG CTGGCCCTGCTGGACGAGGAATGCTGGTTCCCCAAAGCCACGGACAAGTCTTTCGTGGAGAAGCTGTGCACGGAGCAGGGCAGCCACCCCAAGTTCCAGA AGCCCAAGCAGCTCAAGGACAAGACTGAGTTCTCCATCATCCATTATGCTGGGAAGGTGGACTATAATGCGAGTGCCTGGCTGACCAAGAATATGGACCC GCTGAATGACAACGTGACTTCCCTGCTCAATGCCTCCTCCGACAAGTTTGTGGCCGACCTGTGGAAGGACGTGGACCGCATCGTGGGCCTGGACCAGATG GCCAAGATGACGGAGAGCTCGCTGCCCAGCGCCTCCAAGACCAAGAAGGGCATGTTCCGCACAGTGGGGCAGCTGTACAAGGAGCAGCTGGGCAAGCTGA TGACCACGCTACGCAACACCACGCCCAACTTCGTGCGCTGCATCATCCCCAACCACGAGAAGAGGTCCGGCAAGCTGGATGCGTTCCTGGTGCTGGAGCA GCTGCGGTGCAATGGGGTGCTGGAAGGCATTCGCATCTGCCGGCAGGGCTTCCCCAACCGGATCGTCTTCCAGGAGTTCCGCCAACGCTACGAGATCCTG GCGGCGAATGCCATCCCCAAAGGCTTCATGGACGGGAAGCAGGCCTGCATTCTCATGATCAAAGCCCTGGAACTTGACCCCAACTTATACAGGATAGGGC AGAGCAAAATCTTCTTCCGAACTGGCGTCCTGGCCCACCTAGAGGAGGAGCGAGATTTGAAGATCACCGATGTCATCATGGCCTTCCAGGCGATGTGTCG TGGCTACTTGGCCAGAAAGGCTTTTGCCAAGAGGCAGCAGCAGCTGACCGCCATGAAGGTGATTCAGAGGAACTGCGCCGCCTACCTCAAGCTGCGGAAC TGGCAGTGGTGGAGGCTTTTCACCAAAGTGAAGCCACTGCTGCAGGTGACACGGCAGGAGGAGGAGATGCAGGCCAAGGAGGATGAACTGCAGAAGACCA AGGAGCGGCAGCAGAAGGCAGAGAATGAGCTTAAGGAGCTGGAACAGAAGCACTCGCAGCTGACCGAGGAGAAGAACCTGCTACAGGAACAGCTGCAGGC AGAGACAGAGCTGTATGCAGAGGCTGAGGAGATGCGGGTGCGGCTGGCGGCCAAGAAGCAGGAGCTGGAGGAGATACTGCATGAGATGGAGGCCCGCCTG GAGGAGGAGGAAGACAGGGGCCAGCAGCTACAGGCTGAAAGGAAGAAGATGGCCCAGCAGATGCTGGACCTTGAAGAACAGCTGGAGGAGGAGGAAGCTG CCAGGCAGAAGCTGCAACTTGAGAAGGTCACGGCTGAGGCCAAGATCAAGAAACTGGAGGATGAGATCCTGGTCATGGATGATCAGAACAATAAACTATC AAAAGAACGAAAACTCCTTGAGGAGAGGATTAGTGACTTAACGACAAATCTTGCAGAAGAGGAAGAAAAGGCCAAGAATCTTACCAAGCTGAAAAACAAG CATGAATCTATGATTTCAGAACTGGAAGTGCGGCTAAAGAAGGAAGAGAAGAGCCGACAGGAGCTGGAGAAGCTGAAACGGAAGCTGGAGGGTGATGCCA GCGACTTCCACGAGCAGATCGCTGACCTCCAGGCGCAGATCGCAGAGCTCAAGATGCAGCTGGCCAAGAAGGAGGAGGAGCTGCAGGCGGCCCTGGCCAG GCTTGACGATGAAATCGCTCAGAAGAACAATGCCCTGAAGAAGATCCGGGAGCTGGAGGGCCACATCTCAGACCTCCAGGAGGACCTGGACTCAGAGCGG GCCGCCAGGAACAAGGCTGAAAAGCAGAAGCGAGACCTCGGCGAGGAGCTGGAGGCCCTAAAGACAGAGCTGGAAGACACACTGGACAGCACAGCCACTC AGCAGGAGCTCAGGGCCAAGAGGGAGCAGGAGGTGACGGTGCTGAAGAAGGCCCTGGATGAAGAGACGCGGTCCCATGAGGCTCAGGTCCAGGAGATGAG GCAGAAACACGCACAGGCGGTGGAGGAGCTCACAGAGCAGCTTGAGCAGTTCAAGAGGGCCAAGGCGAACCTAGACAAGAATAAGCAGACGCTGGAGAAA GAGAACGCAGACCTGGCCGGGGAGCTGCGGGTCCTGGGCCAGGCCAAGCAGGAGGTGGAACATAAGAAGAAGAAGCTGGAGGCGCAGGTGCAGGAGCTGC AGTCCAAGTGCAGCGATGGGGAGCGGGCCCGGGCGGAGCTCAATGACAAAGTCCACAAGCTGCAGAATGAAGTTGAGAGCGTCACAGGGATGCTTAACGA GGCCGAGGGGAAGGCCATTAAGCTGGCCAAGGACGTGGCGTCCCTCAGTTCCCAGCTCCAGGACACCCAGGAGCTGCTTCAAGAAGAAACCCGGCAGAAG CTCAACGTGTCTACGAAGCTGCGCCAGCTGGAGGAGGAGCGGAACAGCCTGCAAGACCAGCTGGACGAGGAGATGGAGGCCAAGCAGAACCTGGAGCGCC ACATCTCCACTCTCAACATCCAGCTCTCCGACTCGAAGAAGAAGCTGCAGGACTTTGCCAGCACCGTGGAAGCTCTGGAAGAGGGGAAGAAGAGGTTCCA GAAGGAGATCGAGAACCTCACCCAGCAGTACGAGGAGAAGGCGGCCGCTTATGATAAACTGGAAAAGACCAAGAACAGGCTTCAGCAGGAGCTGGACGAC CTGGTTGTTGATTTGGACAACCAGCGGCAACTCGTGTCCAACCTGGAAAAGAAGCAGAGGAAATTTGATCAGTTGTTAGCCGAGGAGAAAAACATCTCTT CCAAATACGCGGATGAGAGGGACAGAGCTGAGGCAGAAGCCAGGGAGAAGGAAACCAAGGCCCTGTCCCTGGCTCGGGCCCTTGAAGAGGCCTTGGAAGC CAAAGAGGAACTCGAGCGGACCAACAAAATGCTCAAAGCCGAAATGGAAGACCTGGTCAGCTCCAAGGATGACGTGGGCAAGAACGTCCATGAGCTGGAG AAGTCCAAGCGGGCCCTGGAGACCCAGATGGAGGAGATGAAGACGCAGCTGGAAGAGCTGGAGGACGAGCTGCAAGCCACGGAGGACGCCAAACTGCGGC TGGAAGTCAACATGCAGGCGCTCAAGGGCCAGTTCGAAAGGGATCTCCAAGCCCGGGACGAGCAGAATGAGGAGAAGAGGAGGCAACTGCAGAGACAGCT TCACGAGTATGAGACGGAACTGGAAGACGAGCGAAAGCAACGTGCCCTGGCAGCTGCAGCAAAGAAGAAGCTGGAAGGGGACCTGAAAGACCTGGAGCTT CAGGCCGACTCTGCCATCAAGGGGAGGGAGGAAGCCATCAAGCAGCTACGCAAACTGCAGGCTCAGATGAAGGACTTTCAAAGAGAGCTGGAAGATGCCC GTGCCTCCAGAGATGAGATCTTTGCCACAGCCAAAGAGAATGAGAAGAAAGCCAAGAGCTTGGAAGCAGACCTCATGCAGCTACAAGAGGACCTCGCCGC CGCTGAGAGGGCTCGCAAACAAGCGGACCTCGAGAAGGAGGAACTGGCAGAGGAGCTGGCCAGTAGCCTGTCGGGAAGGAACGCACTCCAGGACGAGAAG CGCCGCCTGGAGGCCCGGATCGCCCAGCTGGAGGAGGAGCTGGAGGAGGAGCAGGGCAACATGGAGGCCATGAGCGACCGGGTCCGCAAAGCCACACAGC AGGCCGAGCAGCTCAGCAACGAGCTGGCCACAGAGCGCAGCACGGCCCAGAAGAATGAGAGTGCCCGGCAGCAGCTCGAGCGGCAGAACAAGGAGCTCCG GAGCAAGCTCCACGAGATGGAGGGGGCCGTCAAGTCCAAGTTCAAGTCCACCATCGCGGCGCTGGAGGCCAAGATTGCACAGCTGGAGGAGCAGGTCGAG CAGGAGGCCAGAGAGAAACAGGCGGCCACCAAGTCGCTGAAGCAGAAAGACAAGAAGCTGAAGGAAATCTTGCTGCAGGTGGAGGACGAGCGCAAGATGG CCGAGCAGTACAAGGAGCAGGCAGAGAAAGGCAATGCCAGGGTCAAGCAGCTCAAGAGGCAGCTGGAGGAGGCAGAGGAGGAGTCCCAGCGCATCAACGC CAACCGCAGGAAGCTGCAGCGGGAGCTGGATGAGGCCACGGAGAGCAACGAGGCCATGGGCCGCGAGGTGAACGCACTCAAGAGCAAGCTCAGGCGAGGA AACGAGACCTCTTTCGTTCCTTCTAGAAGGTCTGGAGGACGTAGAGTTATTGAAAATGCAGATGGTTCTGAGGAGGAAACGGACACTCGAGACGCAGACT TCAATGGAACCAAGGCCAGTGAATAAGCAACTTTCTACAGTTTTGCACCACGGCAAGAAAACCAAAAACCAAAACAAACAAACAAAAAAAACCCAACAAC AACCCAGAACAAAGCAAAACCCAGCAGACTGTACTTAGCATTGTCTAAATCCATTCTCAAATTCCAAATATCACAGACACCCCTCACACAAGGAATATAA AAACCACCACCCTCCAGCCTGGGCAACGTAGTAAAACCTCATCTATACAAGAATTTAAAAATAAGCTGGGCGTGGTGGTACACACCTGTGGTCCCAGCTA CTAGGGAGGCTGAGCCAGGAAGAACGCTCCAGCCCAGGACTTCGAGGCTGCAATGAGCTATAATTGCATCATTGCACTCCAGCCTGGGCAACAGAGACCC TGTCTCAACCACCACCACCACCACCACCCCTACTACCCCTGTATTCAAGGTAAAAATTGAAGTTTGTATGATGTAAGAGATGAGAAAAACCCAACAGGAA ACACAGACACATCCTCCAGTTCTATCAATGGATTGTGCAGACACTGAGTTTTTAGAAAAACATATCCACGGTAACCGGTCCCTGGCAATTCTGTTTACAT GAAATGGGGAGAAAGTCACCGAAATGGGTGCCGCCGGCCCCCACTCCCAATTCATTCCCTAACCTGCAAACCTTTCCAACTTCTCACGTCAGGCCTTTGA GAATTCTTTCCCCCTCTCCTGGTTTCCACACCTCAGACACGCACAGTTCACCAAGTGCCTTCTGTAGTCACATGAATTGAAAAGGAGACGCTGCTCCCAC GGAGGGGAGCAGGAATGCTGCACTGTTTACACCCTGACTGTGCTTAAAAACACTTTCACTAATAAATGGTTATAAATCACAA

refseq_MYH11.F1 (forward primer)
refseq_MYH11.R1 (reverse primer)
Single exon skipping

Frame 0

GRFGRSGLGGASDPSSPSSFLSTSPPSWRSGTN KAPWRRRANSVTMRSSSLWTKTSSTAQWPRLTGP PRDSSGSPRRSRASRQPALRRRRGMRWLWSWWR MARRSRLGKMTSRR*THPSSPRWRTWRS*RAST KPPCYTT*GSGTSQG*YIRTLASSAWWSTPINTC PSTRRRSSTCTRARRGTRCRLTSTPSQTRPTGA CFKIGRTSPFYAQASLEPGKPKTPRRSFSTWPW WPPPTRARKTQVSRESWKSSFYKQTRFWRLSATP KQ*RTTTPHDSANSSASTSTSRVTSWEPTLRPI C*KNHGQFAKPETRGHSTSFTT*LLEPRRR*EV TCFWRASTTTPSSPMALCPSQQPRMMRCSRKPWR PWQSWVSARRSSYPY*RWYHRSCSLEISSSRRK ETQTRRPCQITQLLRKFATSWELM*QISPDPSS LLVSRLGEMWYRKLRQKNRLTLL*RLWPRQHMSA FSAGYSPA*TKPWTRPIGKGLPSWGSWI*LDLR SLR*TPSSSCASTTPTRSCSSSSTTPCSSWSRR STSARASSGTSSTLGWTYSPASSSSSDRTTLQVC WPCWTRNAGSPKPRTSLSWRSCARSRAATPSSR SPSSSRTRLSSPSSIMLGRWTIMRVPG*PRIWT R*MTT*LPCSMPPPTSLWPTCGRTWTASWAWTRW PR*RRARCPAPPRPRRACSAQWGSCTRSSWAS* *PRYATPRPTSCAASSPTTRRGPASWMRSWCWS SCGAMGCWKAFASAGRASPTGSSSRSSANATRSW RRMPSPKASWTGSRPAFS*SKPWNLTPTYTG*G RAKSSSELASWPT*RRSEI*RSPMSSWPSRRCV VATWPERLLPRGSSS*PP*R*FRGTAPPTSSCGT GSGGGFSPK*SHCCR*HGRRRRCRPRRMNCRRP RSGSRRQRMSLRSWNRSTRS*PRRRTCYRNSCR QRQSCMQRLRRCGCGWRPRSRSWRRYCMRWRPAW RRRKTGASSYRLKGRRWPSRCWTLKNSWRRRKL PGRSCNLRRSRLRPRSRNWRMRSWSWMIRTINY QKNENSLRRGLVT*RQILQKRKKRPRILPS*KTS MNL*FQNWKCG*RRKRRADRSWRS*NGSWRVMP ATSTSRSLTSRRRSQSSRCSWPRRRRSCRRPWP GLTMKSLRRTMP*RRSGSWRATSQTSRRTWTQSG PPGTRLKSRSETSARSWRP*RQSWKTHWTAQPL SRSSGPRGSRR*RC*RRPWMKRRGPMRLRSRR* GRNTHRRWRSSQSSLSSSRGPRRT*TRISRRWRK RTQTWPGSCGSWARPSRRWNIRRRSWRRRCRSC SPSAAMGSGPGRSSMTKSTSCRMKLRASQGCLT RPRGRPLSWPRTWRPSVPSSRTPRSCFKKKPGRS STCLRSCASWRRSGTACKTSWTRRWRPSRTWSA TSPLSTSSSPTRRRSCRTLPAPWKLWKRGRRGS RRRSRTSPSSTRRRRPLMINWKRPRTGFSRSWTT WLLIWTTSGNSCPTWKRSRGNLISC*PRRKTSL PNTRMRGTELRQKPGRRKPRPCPWLGPLKRPWK PKRNSSGPTKCSKPKWKTWSAPRMTWARTSMSWR SPSGPWRPRWRR*RRSWKSWRTSCKPRRTPNCG WKSTCRRSRASSKGISKPGTSRMRRRGGNCRDS FTSMRRNWKTSESNVPWQLQQRRSWKGT*KTWSF RPTLPSRGGRKPSSSYANCRLR*RTFKESWKMP VPPEMRSLPQPKRMRRKPRAWKQTSCSYKRTSP PLRGLANKRTSRRRNWQRSWPVACREGTHSRTRS AAWRPGSPSWRRSWRRSRATWRP*ATGSAKPHS RPSSSATSWPQSAARPRRMRVPGSSSSGRTRSS GASSTRWRGPSSPSSSPPSRRWRPRLHSWRSRSS RRPERNRRPPSR*SRKTRS*RKSCCRWRTSARW PSSTRSRQRKAMPGSSSSRGSWRRQRRSPSAST PTAGSCSGSWMRPRRATRPWAAR*THSRASSGEE TRPLSFLLEGLEDVELLKMQMVLRRKRTLETQT SMEPRPVNKQLSTVLHHGKKTKNQNKQTKKTQQ QPRTKQNPADCT*HCLNPFSNSKYHRHPSHKEYK NHHPPAWAT**NLIYTRI*K*AGRGGTHLWSQL LGRLSQEERSSPGLRGCNEL*LHHCTPAWATET LSQPPPPPPPLLPLYSR*KLKFV*CKR*EKPNRK HRHILQFYQWIVQTLSF*KNISTVTGPWQFCLH EMGRKSPKWVPPAPTPNSFPNLQTFPTSHVRPL RILSPSPGFHTSDTHSSPSAFCSHMN*KGDAAPT EGSRNAALFTP*LCLKTLSLINGYKSQ

Frame 1

GDLDAPAWEVRQIRARHPVSSPLVPPVGDLGPT RHHGAEGPTQ*R*EVPLCGQKLHQQPSGPG*LG RQETRLGPLGEAGLRGSQH*GGEGG*GGCGAGGE WQEGHGWER*HPEDEPTQVLQGGGHGGADVPQR SLRATQPEGAVLLRANIYVLWPLLRGGQPL*TP AHLLGEDRRHVQGQEEARDAASHLRHRRHGLPEH ASRSGGPVHSMHRRVWSRENRKHQEGHSVPGRG GLLPQGQERHKYHGRAGKAASTSKPDSGGFRQR QNSEERQLLTIRQIHPHQLRRHGLHRGSQH*DLS ARKITGNSPSQRREDIPHLLLHDCWSQGEDEK* LAFGGLQQLHLPLQWLCAHPSSPG**DVPGNRG GHGNHGFQRGGAAIHIEGGIIGPAAWKYRLQEGK KHRPGVHAR*HSCSESLPPHGN*CDRFHQIHPH SSYQGWARCGTESSDKRTG*LCCRGFGQGNI*A PFPLDTHPREQSPGQDPSARGFLPGDPGYSWI*D L*GELLRAAVHQLHQREAAAALQPHHVHPGAGG VPARGHRVELHRLWAGPTALHRAHRATEQPSRC AGPAGRGMLVPQSHGQVFRGEAVHGAGQPPQVPE AQAAQGQD*VLHHPLCWEGGL*CECLADQEYGP AE*QRDFPAQCLLRQVCGRPVEGRGPHRGPGPD GQDDGELAAQRLQDQEGHVPHSGAAVQGAAGQAD DHATQHHAQLRALHHPQPREEVRQAGCVPGAGA AAVQWGAGRHSHLPAGLPQPDRLPGVPPTLRDP GGECHPQRLHGREAGLHSHDQSPGT*PQLIQDRA EQNLLPNWRPGPPRGGARFEDHRCHHGLPGDVS WLLGQKGFCQEAAAADRHEGDSEELRRLPQAAE LAVVEAFHQSEATAAGDTAGGGDAGQGG*TAEDQ GAAAEGRE*A*GAGTEALAADRGEEPATGTAAG RDRAVCRG*GDAGAAGGQEAGAGGDTA*DGGPP GGGGRQGPAATG*KEEDGPADAGP*RTAGGGGSC QAEAAT*EGHG*GQDQETGG*DPGHG*SEQ*TI KRTKTP*GED**LNDKSCRRGRKGQESYQAEKQ A*IYDFRTGSAAKEGREEPTGAGEAETEAGG*CQ RLPRADR*PPGADRRAQDAAGQEGGGAAGGPGQ A*R*NRSEEQCPEEDPGAGGPHLRPPGGPGLRA GRQEQG*KAEARPRRGAGGPKDRAGRHTGQHSHS AGAQGQEGAGGDGAEEGPG*RDAVP*GSGPGDE AETRTGGGGAHRAA*AVQEGQGEPRQE*ADAGE RERRPGRGAAGPGPGQAGGGT*EEEAGGAGAGAA VQVQRWGAGPGGAQ*QSPQAAE*S*ERHRDA*R GRGEGH*AGQGRGVPQFPAPGHPGAASRRNPAE AQRVYEAAPAGGGAEQPARPAGRGDGGQAEPGAP HLHSQHPALRLEEEAAGLCQHRGSSGRGEEEVP EGDREPHPAVRGEGGRL**TGKDQEQASAGAGR PGC*FGQPAATRVQPGKEAEEI*SVVSRGEKHLF QIRG*EGQS*GRSQGEGNQGPVPGSGP*RGLGS QRGTRADQQNAQSRNGRPGQLQG*RGQERP*AG EVQAGPGDPDGGDEDAAGRAGGRAASHGGRQTAA GSQHAGAQGPVRKGSPSPGRAE*GEEEATAETA SRV*DGTGRRAKATCPGSCSKEEAGRGPERPGA SGRLCHQGEGGSHQAATQTAGSDEGLSKRAGRCP CLQR*DLCHSQRE*EESQELGSRPHAATRGPRR R*EGSQTSGPREGGTGRGAGQ*PVGKERTPGRE APPGGPDRPAGGGAGGGAGQHGGHERPGPQSHTA GRAAQQRAGHRAQHGPEE*ECPAAARAAEQGAP EQAPRDGGGRQVQVQVHHRGAGGQDCTAGGAGR AGGQRETGGHQVAEAERQEAEGNLAAGGGRAQDG RAVQGAGRERQCQGQAAQEAAGGGRGGVPAHQR QPQEAAAGAG*GHGEQRGHGPRGERTQEQAQAR KRDLFRSF*KVWRT*SY*KCRWF*GGNGHSRRRL QWNQGQ*ISNFLQFCTTARKPKTKTNKQKKPNN NPEQSKTQQTVLSIV*IHSQIPNITDTPHTRNI KTTTLQPGQRSKTSSIQEFKNKLGVVVHTCGPSY *GG*ARKNAPAQDFEAAMSYNCIIALQPGQQRP CLNHHHHHHPYYPCIQGKN*SLYDVRDEKNPTG NTDTSSSSINGLCRH*VFRKTYPR*PVPGNSVYM KWGESHRNGCRRPPLPIHSLTCKPFQLLTSGL* EFFPPLLVSTPQTRTVHQVPSVVT*IEKETLLP RRGAGMLHCLHPDCA*KHFH**MVINH

Frame 2

EIWTLRPGRCVRSELAIQFPLH*SPQLEIWDQQ GTMAQKGQLSDDEKFLFVDKNFINSPVAQADWA AKRLVWVPSEKQGFEAASIKEEKGDEVVVELVE NGKKVTVGKDDIQKMNPPKFSKVEDMAELTCLNE ASVLHNLRERYFSGLIYTYSGLFCVVVNPYKHL PIYSEKIVDMYKGKKRHEMPPHIYAIADTAYRS MLQDREDQSILCTGESGAGKTENTKKVIQYLAVV ASSHKGKKDTSITGELEKQLLQANPILEAFGNA KTVKNDNSSRFGKFIRINFDVTGYIVGANIETY LLEKSRAIRQARDERTFHIFYYMIAGAKEKMRSD LLLEGFNNYTFLSNGFVPIPAAQDDEMFQETVE AMAIMGFSEEEQLSILKVVSSVLQLGNIVFKKE RNTDQASMPDNTAAQKVCHLMGINVTDFTRSILT PRIKVGRDVVQKAQTKEQADFAVEALAKATYER LFRWILTRVNKALDKTHRQGASFLGILDIAGFE IFEVNSFEQLCINYTNEKLQQLFNHTMFILEQEE YQREGIEWNFIDFGLDLQPCIELIERPNNPPGV LALLDEECWFPKATDKSFVEKLCTEQGSHPKFQ KPKQLKDKTEFSIIHYAGKVDYNASAWLTKNMDP LNDNVTSLLNASSDKFVADLWKDVDRIVGLDQM AKMTESSLPSASKTKKGMFRTVGQLYKEQLGKL MTTLRNTTPNFVRCIIPNHEKRSGKLDAFLVLEQ LRCNGVLEGIRICRQGFPNRIVFQEFRQRYEIL AANAIPKGFMDGKQACILMIKALELDPNLYRIG QSKIFFRTGVLAHLEEERDLKITDVIMAFQAMCR GYLARKAFAKRQQQLTAMKVIQRNCAAYLKLRN WQWWRLFTKVKPLLQVTRQEEEMQAKEDELQKT KERQQKAENELKELEQKHSQLTEEKNLLQEQLQA ETELYAEAEEMRVRLAAKKQELEEILHEMEARL EEEEDRGQQLQAERKKMAQQMLDLEEQLEEEEA ARQKLQLEKVTAEAKIKKLEDEILVMDDQNNKLS KERKLLEERISDLTTNLAEEEEKAKNLTKLKNK HESMISELEVRLKKEEKSRQELEKLKRKLEGDA SDFHEQIADLQAQIAELKMQLAKKEEELQAALAR LDDEIAQKNNALKKIRELEGHISDLQEDLDSER AARNKAEKQKRDLGEELEALKTELEDTLDSTAT QQELRAKREQEVTVLKKALDEETRSHEAQVQEMR QKHAQAVEELTEQLEQFKRAKANLDKNKQTLEK ENADLAGELRVLGQAKQEVEHKKKKLEAQVQEL QSKCSDGERARAELNDKVHKLQNEVESVTGMLNE AEGKAIKLAKDVASLSSQLQDTQELLQEETRQK LNVSTKLRQLEEERNSLQDQLDEEMEAKQNLER HISTLNIQLSDSKKKLQDFASTVEALEEGKKRFQ KEIENLTQQYEEKAAAYDKLEKTKNRLQQELDD LVVDLDNQRQLVSNLEKKQRKFDQLLAEEKNIS SKYADERDRAEAEAREKETKALSLARALEEALEA KEELERTNKMLKAEMEDLVSSKDDVGKNVHELE KSKRALETQMEEMKTQLEELEDELQATEDAKLR LEVNMQALKGQFERDLQARDEQNEEKRRQLQRQL HEYETELEDERKQRALAAAAKKKLEGDLKDLEL QADSAIKGREEAIKQLRKLQAQMKDFQRELEDA RASRDEIFATAKENEKKAKSLEADLMQLQEDLAA AERARKQADLEKEELAEELASSLSGRNALQDEK RRLEARIAQLEEELEEEQGNMEAMSDRVRKATQ QAEQLSNELATERSTAQKNESARQQLERQNKELR SKLHEMEGAVKSKFKSTIAALEAKIAQLEEQVE QEAREKQAATKSLKQKDKKLKEILLQVEDERKM AEQYKEQAEKGNARVKQLKRQLEEAEEESQRINA NRRKLQRELDEATESNEAMGREVNALKSKLRRG NETSFVPSRRSGGRRVIENADGSEEETDTRDAD FNGTKASE*ATFYSFAPRQENQKPKQTNKKNPTT TQNKAKPSRLYLALSKSILKFQISQTPLTQGI* KPPPSSLGNVVKPHLYKNLKISWAWWYTPVVPA TREAEPGRTLQPRTSRLQ*AIIASLHSSLGNRDP VSTTTTTTTPTTPVFKVKIEVCMM*EMRKTQQE TQTHPPVLSMDCADTEFLEKHIHGNRSLAILFT *NGEKVTEMGAAGPHSQFIP*PANLSNFSRQAFE NSFPLSWFPHLRHAQFTKCLL*SHELKRRRCSH GGEQECCTVYTLTVLKNTFTNKWL*IT

Sequence NM_001040114

GGGAGATTTGGACGCTCCGGCCTGGGAGGTGCGTCAGATCCGAGCTCGCCATCCAGTTTCCTCTCCACTAGTCCCCCCAGTTGGAGATCTGGGACCAACA AGGCACCATGGCGCAGAAGGGCCAACTCAGTGACGATGAGAAGTTCCTCTTTGTGGACAAAAACTTCATCAACAGCCCAGTGGCCCAGGCTGACTGGGCC GCCAAGAGACTCGTCTGGGTCCCCTCGGAGAAGCAGGGCTTCGAGGCAGCCAGCATTAAGGAGGAGAAGGGGGATGAGGTGGTTGTGGAGCTGGTGGAGA ATGGCAAGAAGGTCACGGTTGGGAAAGATGACATCCAGAAGATGAACCCACCCAAGTTCTCCAAGGTGGAGGACATGGCGGAGCTGACGTGCCTCAACGA AGCCTCCGTGCTACACAACCTGAGGGAGCGGTACTTCTCAGGGCTAATATATACGTACTCTGGCCTCTTCTGCGTGGTGGTCAACCCCTATAAACACCTG CCCATCTACTCGGAGAAGATCGTCGACATGTACAAGGGCAAGAAGAGGCACGAGATGCCGCCTCACATCTACGCCATCGCAGACACGGCCTACCGGAGCA TGCTTCAAGATCGGGAGGACCAGTCCATTCTATGCACAGGCGAGTCTGGAGCCGGGAAAACCGAAAACACCAAGAAGGTCATTCAGTACCTGGCCGTGGT GGCCTCCTCCCACAAGGGCAAGAAAGACACAAGTATCACGCAAGGCCCATCTTTTGCCTACGGAGAGCTGGAAAAGCAGCTTCTACAAGCAAACCCGATT CTGGAGGCTTTCGGCAACGCCAAAACAGTGAAGAACGACAACTCCTCACGATTCGGCAAATTCATCCGCATCAACTTCGACGTCACGGGTTACATCGTGG GAGCCAACATTGAGACCTATCTGCTAGAAAAATCACGGGCAATTCGCCAAGCCAGAGACGAGAGGACATTCCACATCTTTTACTACATGATTGCTGGAGC CAAGGAGAAGATGAGAAGTGACTTGCTTTTGGAGGGCTTCAACAACTACACCTTCCTCTCCAATGGCTTTGTGCCCATCCCAGCAGCCCAGGATGATGAG ATGTTCCAGGAAACCGTGGAGGCCATGGCAATCATGGGTTTCAGCGAGGAGGAGCAGCTATCCATATTGAAGGTGGTATCATCGGTCCTGCAGCTTGGAA ATATCGTCTTCAAGAAGGAAAGAAACACAGACCAGGCGTCCATGCCAGATAACACAGCTGCTCAGAAAGTTTGCCACCTCATGGGAATTAATGTGACAGA TTTCACCAGATCCATCCTCACTCCTCGTATCAAGGTTGGGCGAGATGTGGTACAGAAAGCTCAGACAAAAGAACAGGCTGACTTTGCTGTAGAGGCTTTG GCCAAGGCAACATATGAGCGCCTTTTCCGCTGGATACTCACCCGCGTGAACAAAGCCCTGGACAAGACCCATCGGCAAGGGGCTTCCTTCCTGGGGATCC TGGATATAGCTGGATTTGAGATCTTTGAGGTGAACTCCTTCGAGCAGCTGTGCATCAACTACACCAACGAGAAGCTGCAGCAGCTCTTCAACCACACCAT GTTCATCCTGGAGCAGGAGGAGTACCAGCGCGAGGGCATCGAGTGGAACTTCATCGACTTTGGGCTGGACCTACAGCCCTGCATCGAGCTCATCGAGCGA CCGAACAACCCTCCAGGTGTGCTGGCCCTGCTGGACGAGGAATGCTGGTTCCCCAAAGCCACGGACAAGTCTTTCGTGGAGAAGCTGTGCACGGAGCAGG GCAGCCACCCCAAGTTCCAGAAGCCCAAGCAGCTCAAGGACAAGACTGAGTTCTCCATCATCCATTATGCTGGGAAGGTGGACTATAATGCGAGTGCCTG GCTGACCAAGAATATGGACCCGCTGAATGACAACGTGACTTCCCTGCTCAATGCCTCCTCCGACAAGTTTGTGGCCGACCTGTGGAAGGACGTGGACCGC ATCGTGGGCCTGGACCAGATGGCCAAGATGACGGAGAGCTCGCTGCCCAGCGCCTCCAAGACCAAGAAGGGCATGTTCCGCACAGTGGGGCAGCTGTACA AGGAGCAGCTGGGCAAGCTGATGACCACGCTACGCAACACCACGCCCAACTTCGTGCGCTGCATCATCCCCAACCACGAGAAGAGGTCCGGCAAGCTGGA TGCGTTCCTGGTGCTGGAGCAGCTGCGGTGCAATGGGGTGCTGGAAGGCATTCGCATCTGCCGGCAGGGCTTCCCCAACCGGATCGTCTTCCAGGAGTTC CGCCAACGCTACGAGATCCTGGCGGCGAATGCCATCCCCAAAGGCTTCATGGACGGGAAGCAGGCCTGCATTCTCATGATCAAAGCCCTGGAACTTGACC CCAACTTATACAGGATAGGGCAGAGCAAAATCTTCTTCCGAACTGGCGTCCTGGCCCACCTAGAGGAGGAGCGAGATTTGAAGATCACCGATGTCATCAT GGCCTTCCAGGCGATGTGTCGTGGCTACTTGGCCAGAAAGGCTTTTGCCAAGAGGCAGCAGCAGCTGACCGCCATGAAGGTGATTCAGAGGAACTGCGCC GCCTACCTCAAGCTGCGGAACTGGCAGTGGTGGAGGCTTTTCACCAAAGTGAAGCCACTGCTGCAGGTGACACGGCAGGAGGAGGAGATGCAGGCCAAGG AGGATGAACTGCAGAAGACCAAGGAGCGGCAGCAGAAGGCAGAGAATGAGCTTAAGGAGCTGGAACAGAAGCACTCGCAGCTGACCGAGGAGAAGAACCT GCTACAGGAACAGCTGCAGGCAGAGACAGAGCTGTATGCAGAGGCTGAGGAGATGCGGGTGCGGCTGGCGGCCAAGAAGCAGGAGCTGGAGGAGATACTG CATGAGATGGAGGCCCGCCTGGAGGAGGAGGAAGACAGGGGCCAGCAGCTACAGGCTGAAAGGAAGAAGATGGCCCAGCAGATGCTGGACCTTGAAGAAC AGCTGGAGGAGGAGGAAGCTGCCAGGCAGAAGCTGCAACTTGAGAAGGTCACGGCTGAGGCCAAGATCAAGAAACTGGAGGATGAGATCCTGGTCATGGA TGATCAGAACAATAAACTATCAAAAGAACGAAAACTCCTTGAGGAGAGGATTAGTGACTTAACGACAAATCTTGCAGAAGAGGAAGAAAAGGCCAAGAAT CTTACCAAGCTGAAAAACAAGCATGAATCTATGATTTCAGAACTGGAAGTGCGGCTAAAGAAGGAAGAGAAGAGCCGACAGGAGCTGGAGAAGCTGAAAC GGAAGCTGGAGGGTGATGCCAGCGACTTCCACGAGCAGATCGCTGACCTCCAGGCGCAGATCGCAGAGCTCAAGATGCAGCTGGCCAAGAAGGAGGAGGA GCTGCAGGCGGCCCTGGCCAGGCTTGACGATGAAATCGCTCAGAAGAACAATGCCCTGAAGAAGATCCGGGAGCTGGAGGGCCACATCTCAGACCTCCAG GAGGACCTGGACTCAGAGCGGGCCGCCAGGAACAAGGCTGAAAAGCAGAAGCGAGACCTCGGCGAGGAGCTGGAGGCCCTAAAGACAGAGCTGGAAGACA CACTGGACAGCACAGCCACTCAGCAGGAGCTCAGGGCCAAGAGGGAGCAGGAGGTGACGGTGCTGAAGAAGGCCCTGGATGAAGAGACGCGGTCCCATGA GGCTCAGGTCCAGGAGATGAGGCAGAAACACGCACAGGCGGTGGAGGAGCTCACAGAGCAGCTTGAGCAGTTCAAGAGGGCCAAGGCGAACCTAGACAAG AATAAGCAGACGCTGGAGAAAGAGAACGCAGACCTGGCCGGGGAGCTGCGGGTCCTGGGCCAGGCCAAGCAGGAGGTGGAACATAAGAAGAAGAAGCTGG AGGCGCAGGTGCAGGAGCTGCAGTCCAAGTGCAGCGATGGGGAGCGGGCCCGGGCGGAGCTCAATGACAAAGTCCACAAGCTGCAGAATGAAGTTGAGAG CGTCACAGGGATGCTTAACGAGGCCGAGGGGAAGGCCATTAAGCTGGCCAAGGACGTGGCGTCCCTCAGTTCCCAGCTCCAGGACACCCAGGAGCTGCTT CAAGAAGAAACCCGGCAGAAGCTCAACGTGTCTACGAAGCTGCGCCAGCTGGAGGAGGAGCGGAACAGCCTGCAAGACCAGCTGGACGAGGAGATGGAGG CCAAGCAGAACCTGGAGCGCCACATCTCCACTCTCAACATCCAGCTCTCCGACTCGAAGAAGAAGCTGCAGGACTTTGCCAGCACCGTGGAAGCTCTGGA AGAGGGGAAGAAGAGGTTCCAGAAGGAGATCGAGAACCTCACCCAGCAGTACGAGGAGAAGGCGGCCGCTTATGATAAACTGGAAAAGACCAAGAACAGG CTTCAGCAGGAGCTGGACGACCTGGTTGTTGATTTGGACAACCAGCGGCAACTCGTGTCCAACCTGGAAAAGAAGCAGAGGAAATTTGATCAGTTGTTAG CCGAGGAGAAAAACATCTCTTCCAAATACGCGGATGAGAGGGACAGAGCTGAGGCAGAAGCCAGGGAGAAGGAAACCAAGGCCCTGTCCCTGGCTCGGGC CCTTGAAGAGGCCTTGGAAGCCAAAGAGGAACTCGAGCGGACCAACAAAATGCTCAAAGCCGAAATGGAAGACCTGGTCAGCTCCAAGGATGACGTGGGC AAGAACGTCCATGAGCTGGAGAAGTCCAAGCGGGCCCTGGAGACCCAGATGGAGGAGATGAAGACGCAGCTGGAAGAGCTGGAGGACGAGCTGCAAGCCA CGGAGGACGCCAAACTGCGGCTGGAAGTCAACATGCAGGCGCTCAAGGGCCAGTTCGAAAGGGATCTCCAAGCCCGGGACGAGCAGAATGAGGAGAAGAG GAGGCAACTGCAGAGACAGCTTCACGAGTATGAGACGGAACTGGAAGACGAGCGAAAGCAACGTGCCCTGGCAGCTGCAGCAAAGAAGAAGCTGGAAGGG GACCTGAAAGACCTGGAGCTTCAGGCCGACTCTGCCATCAAGGGGAGGGAGGAAGCCATCAAGCAGCTACGCAAACTGCAGGCTCAGATGAAGGACTTTC AAAGAGAGCTGGAAGATGCCCGTGCCTCCAGAGATGAGATCTTTGCCACAGCCAAAGAGAATGAGAAGAAAGCCAAGAGCTTGGAAGCAGACCTCATGCA GCTACAAGAGGACCTCGCCGCCGCTGAGAGGGCTCGCAAACAAGCGGACCTCGAGAAGGAGGAACTGGCAGAGGAGCTGGCCAGTAGCCTGTCGGGAAGG AACGCACTCCAGGACGAGAAGCGCCGCCTGGAGGCCCGGATCGCCCAGCTGGAGGAGGAGCTGGAGGAGGAGCAGGGCAACATGGAGGCCATGAGCGACC GGGTCCGCAAAGCCACACAGCAGGCCGAGCAGCTCAGCAACGAGCTGGCCACAGAGCGCAGCACGGCCCAGAAGAATGAGAGTGCCCGGCAGCAGCTCGA GCGGCAGAACAAGGAGCTCCGGAGCAAGCTCCACGAGATGGAGGGGGCCGTCAAGTCCAAGTTCAAGTCCACCATCGCGGCGCTGGAGGCCAAGATTGCA CAGCTGGAGGAGCAGGTCGAGCAGGAGGCCAGAGAGAAACAGGCGGCCACCAAGTCGCTGAAGCAGAAAGACAAGAAGCTGAAGGAAATCTTGCTGCAGG TGGAGGACGAGCGCAAGATGGCCGAGCAGTACAAGGAGCAGGCAGAGAAAGGCAATGCCAGGGTCAAGCAGCTCAAGAGGCAGCTGGAGGAGGCAGAGGA GGAGTCCCAGCGCATCAACGCCAACCGCAGGAAGCTGCAGCGGGAGCTGGATGAGGCCACGGAGAGCAACGAGGCCATGGGCCGCGAGGTGAACGCACTC AAGAGCAAGCTCAGGCGAGGAAACGAGACCTCTTTCGTTCCTTCTAGAAGGTCTGGAGGACGTAGAGTTATTGAAAATGCAGATGGTTCTGAGGAGGAAA CGGACACTCGAGACGCAGACTTCAATGGAACCAAGGCCAGTGAATAAGCAACTTTCTACAGTTTTGCACCACGGCAAGAAAACCAAAAACCAAAACAAAC AAACAAAAAAAACCCAACAACAACCCAGAACAAAGCAAAACCCAGCAGACTGTACTTAGCATTGTCTAAATCCATTCTCAAATTCCAAATATCACAGACA CCCCTCACACAAGGAATATAAAAACCACCACCCTCCAGCCTGGGCAACGTAGTAAAACCTCATCTATACAAGAATTTAAAAATAAGCTGGGCGTGGTGGT ACACACCTGTGGTCCCAGCTACTAGGGAGGCTGAGCCAGGAAGAACGCTCCAGCCCAGGACTTCGAGGCTGCAATGAGCTATAATTGCATCATTGCACTC CAGCCTGGGCAACAGAGACCCTGTCTCAACCACCACCACCACCACCACCCCTACTACCCCTGTATTCAAGGTAAAAATTGAAGTTTGTATGATGTAAGAG ATGAGAAAAACCCAACAGGAAACACAGACACATCCTCCAGTTCTATCAATGGATTGTGCAGACACTGAGTTTTTAGAAAAACATATCCACGGTAACCGGT CCCTGGCAATTCTGTTTACATGAAATGGGGAGAAAGTCACCGAAATGGGTGCCGCCGGCCCCCACTCCCAATTCATTCCCTAACCTGCAAACCTTTCCAA CTTCTCACGTCAGGCCTTTGAGAATTCTTTCCCCCTCTCCTGGTTTCCACACCTCAGACACGCACAGTTCACCAAGTGCCTTCTGTAGTCACATGAATTG AAAAGGAGACGCTGCTCCCACGGAGGGGAGCAGGAATGCTGCACTGTTTACACCCTGACTGTGCTTAAAAACACTTTCACTAATAAATGGTTATAAATCA CAA

refseq_MYH11.F1 (forward primer)
refseq_MYH11.R1 (reverse primer)
Single exon skipping

Frame 0

GRFGRSGLGGASDPSSPSSFLSTSPPSWRSGTN KAPWRRRANSVTMRSSSLWTKTSSTAQWPRLTGP PRDSSGSPRRSRASRQPALRRRRGMRWLWSWWR MARRSRLGKMTSRR*THPSSPRWRTWRS*RAST KPPCYTT*GSGTSQG*YIRTLASSAWWSTPINTC PSTRRRSSTCTRARRGTRCRLTSTPSQTRPTGA CFKIGRTSPFYAQASLEPGKPKTPRRSFSTWPW WPPPTRARKTQVSRKAHLLPTESWKSSFYKQTRF WRLSATPKQ*RTTTPHDSANSSASTSTSRVTSW EPTLRPIC*KNHGQFAKPETRGHSTSFTT*LLE PRRR*EVTCFWRASTTTPSSPMALCPSQQPRMMR CSRKPWRPWQSWVSARRSSYPY*RWYHRSCSLE ISSSRRKETQTRRPCQITQLLRKFATSWELM*Q ISPDPSSLLVSRLGEMWYRKLRQKNRLTLL*RLW PRQHMSAFSAGYSPA*TKPWTRPIGKGLPSWGS WI*LDLRSLR*TPSSSCASTTPTRSCSSSSTTP CSSWSRRSTSARASSGTSSTLGWTYSPASSSSSD RTTLQVCWPCWTRNAGSPKPRTSLSWRSCARSR AATPSSRSPSSSRTRLSSPSSIMLGRWTIMRVP G*PRIWTR*MTT*LPCSMPPPTSLWPTCGRTWTA SWAWTRWPR*RRARCPAPPRPRRACSAQWGSCT RSSWAS**PRYATPRPTSCAASSPTTRRGPASW MRSWCWSSCGAMGCWKAFASAGRASPTGSSSRSS ANATRSWRRMPSPKASWTGSRPAFS*SKPWNLT PTYTG*GRAKSSSELASWPT*RRSEI*RSPMSS WPSRRCVVATWPERLLPRGSSS*PP*R*FRGTAP PTSSCGTGSGGGFSPK*SHCCR*HGRRRRCRPR RMNCRRPRSGSRRQRMSLRSWNRSTRS*PRRRT CYRNSCRQRQSCMQRLRRCGCGWRPRSRSWRRYC MRWRPAWRRRKTGASSYRLKGRRWPSRCWTLKN SWRRRKLPGRSCNLRRSRLRPRSRNWRMRSWSW MIRTINYQKNENSLRRGLVT*RQILQKRKKRPRI LPS*KTSMNL*FQNWKCG*RRKRRADRSWRS*N GSWRVMPATSTSRSLTSRRRSQSSRCSWPRRRR SCRRPWPGLTMKSLRRTMP*RRSGSWRATSQTSR RTWTQSGPPGTRLKSRSETSARSWRP*RQSWKT HWTAQPLSRSSGPRGSRR*RC*RRPWMKRRGPM RLRSRR*GRNTHRRWRSSQSSLSSSRGPRRT*TR ISRRWRKRTQTWPGSCGSWARPSRRWNIRRRSW RRRCRSCSPSAAMGSGPGRSSMTKSTSCRMKLR ASQGCLTRPRGRPLSWPRTWRPSVPSSRTPRSCF KKKPGRSSTCLRSCASWRRSGTACKTSWTRRWR PSRTWSATSPLSTSSSPTRRRSCRTLPAPWKLW KRGRRGSRRRSRTSPSSTRRRRPLMINWKRPRTG FSRSWTTWLLIWTTSGNSCPTWKRSRGNLISC* PRRKTSLPNTRMRGTELRQKPGRRKPRPCPWLG PLKRPWKPKRNSSGPTKCSKPKWKTWSAPRMTWA RTSMSWRSPSGPWRPRWRR*RRSWKSWRTSCKP RRTPNCGWKSTCRRSRASSKGISKPGTSRMRRR GGNCRDSFTSMRRNWKTSESNVPWQLQQRRSWKG T*KTWSFRPTLPSRGGRKPSSSYANCRLR*RTF KESWKMPVPPEMRSLPQPKRMRRKPRAWKQTSC SYKRTSPPLRGLANKRTSRRRNWQRSWPVACREG THSRTRSAAWRPGSPSWRRSWRRSRATWRP*AT GSAKPHSRPSSSATSWPQSAARPRRMRVPGSSS SGRTRSSGASSTRWRGPSSPSSSPPSRRWRPRLH SWRSRSSRRPERNRRPPSR*SRKTRS*RKSCCR WRTSARWPSSTRSRQRKAMPGSSSSRGSWRRQR RSPSASTPTAGSCSGSWMRPRRATRPWAAR*THS RASSGEETRPLSFLLEGLEDVELLKMQMVLRRK RTLETQTSMEPRPVNKQLSTVLHHGKKTKNQNK QTKKTQQQPRTKQNPADCT*HCLNPFSNSKYHRH PSHKEYKNHHPPAWAT**NLIYTRI*K*AGRGG THLWSQLLGRLSQEERSSPGLRGCNEL*LHHCT PAWATETLSQPPPPPPPLLPLYSR*KLKFV*CKR *EKPNRKHRHILQFYQWIVQTLSF*KNISTVTG PWQFCLHEMGRKSPKWVPPAPTPNSFPNLQTFP TSHVRPLRILSPSPGFHTSDTHSSPSAFCSHMN* KGDAAPTEGSRNAALFTP*LCLKTLSLINGYKS Q

Frame 1

GDLDAPAWEVRQIRARHPVSSPLVPPVGDLGPT RHHGAEGPTQ*R*EVPLCGQKLHQQPSGPG*LG RQETRLGPLGEAGLRGSQH*GGEGG*GGCGAGGE WQEGHGWER*HPEDEPTQVLQGGGHGGADVPQR SLRATQPEGAVLLRANIYVLWPLLRGGQPL*TP AHLLGEDRRHVQGQEEARDAASHLRHRRHGLPEH ASRSGGPVHSMHRRVWSRENRKHQEGHSVPGRG GLLPQGQERHKYHARPIFCLRRAGKAASTSKPD SGGFRQRQNSEERQLLTIRQIHPHQLRRHGLHRG SQH*DLSARKITGNSPSQRREDIPHLLLHDCWS QGEDEK*LAFGGLQQLHLPLQWLCAHPSSPG** DVPGNRGGHGNHGFQRGGAAIHIEGGIIGPAAWK YRLQEGKKHRPGVHAR*HSCSESLPPHGN*CDR FHQIHPHSSYQGWARCGTESSDKRTG*LCCRGF GQGNI*APFPLDTHPREQSPGQDPSARGFLPGDP GYSWI*DL*GELLRAAVHQLHQREAAAALQPHH VHPGAGGVPARGHRVELHRLWAGPTALHRAHRA TEQPSRCAGPAGRGMLVPQSHGQVFRGEAVHGAG QPPQVPEAQAAQGQD*VLHHPLCWEGGL*CECL ADQEYGPAE*QRDFPAQCLLRQVCGRPVEGRGP HRGPGPDGQDDGELAAQRLQDQEGHVPHSGAAVQ GAAGQADDHATQHHAQLRALHHPQPREEVRQAG CVPGAGAAAVQWGAGRHSHLPAGLPQPDRLPGV PPTLRDPGGECHPQRLHGREAGLHSHDQSPGT*P QLIQDRAEQNLLPNWRPGPPRGGARFEDHRCHH GLPGDVSWLLGQKGFCQEAAAADRHEGDSEELR RLPQAAELAVVEAFHQSEATAAGDTAGGGDAGQG G*TAEDQGAAAEGRE*A*GAGTEALAADRGEEP ATGTAAGRDRAVCRG*GDAGAAGGQEAGAGGDT A*DGGPPGGGGRQGPAATG*KEEDGPADAGP*RT AGGGGSCQAEAAT*EGHG*GQDQETGG*DPGHG *SEQ*TIKRTKTP*GED**LNDKSCRRGRKGQE SYQAEKQA*IYDFRTGSAAKEGREEPTGAGEAET EAGG*CQRLPRADR*PPGADRRAQDAAGQEGGG AAGGPGQA*R*NRSEEQCPEEDPGAGGPHLRPP GGPGLRAGRQEQG*KAEARPRRGAGGPKDRAGRH TGQHSHSAGAQGQEGAGGDGAEEGPG*RDAVP* GSGPGDEAETRTGGGGAHRAA*AVQEGQGEPRQ E*ADAGERERRPGRGAAGPGPGQAGGGT*EEEAG GAGAGAAVQVQRWGAGPGGAQ*QSPQAAE*S*E RHRDA*RGRGEGH*AGQGRGVPQFPAPGHPGAA SRRNPAEAQRVYEAAPAGGGAEQPARPAGRGDGG QAEPGAPHLHSQHPALRLEEEAAGLCQHRGSSG RGEEEVPEGDREPHPAVRGEGGRL**TGKDQEQ ASAGAGRPGC*FGQPAATRVQPGKEAEEI*SVVS RGEKHLFQIRG*EGQS*GRSQGEGNQGPVPGSG P*RGLGSQRGTRADQQNAQSRNGRPGQLQG*RG QERP*AGEVQAGPGDPDGGDEDAAGRAGGRAASH GGRQTAAGSQHAGAQGPVRKGSPSPGRAE*GEE EATAETASRV*DGTGRRAKATCPGSCSKEEAGR GPERPGASGRLCHQGEGGSHQAATQTAGSDEGLS KRAGRCPCLQR*DLCHSQRE*EESQELGSRPHA ATRGPRRR*EGSQTSGPREGGTGRGAGQ*PVGK ERTPGREAPPGGPDRPAGGGAGGGAGQHGGHERP GPQSHTAGRAAQQRAGHRAQHGPEE*ECPAAAR AAEQGAPEQAPRDGGGRQVQVQVHHRGAGGQDC TAGGAGRAGGQRETGGHQVAEAERQEAEGNLAAG GGRAQDGRAVQGAGRERQCQGQAAQEAAGGGRG GVPAHQRQPQEAAAGAG*GHGEQRGHGPRGERT QEQAQARKRDLFRSF*KVWRT*SY*KCRWF*GGN GHSRRRLQWNQGQ*ISNFLQFCTTARKPKTKTN KQKKPNNNPEQSKTQQTVLSIV*IHSQIPNITD TPHTRNIKTTTLQPGQRSKTSSIQEFKNKLGVVV HTCGPSY*GG*ARKNAPAQDFEAAMSYNCIIAL QPGQQRPCLNHHHHHHPYYPCIQGKN*SLYDVR DEKNPTGNTDTSSSSINGLCRH*VFRKTYPR*PV PGNSVYMKWGESHRNGCRRPPLPIHSLTCKPFQ LLTSGL*EFFPPLLVSTPQTRTVHQVPSVVT*I EKETLLPRRGAGMLHCLHPDCA*KHFH**MVINH

Frame 2

EIWTLRPGRCVRSELAIQFPLH*SPQLEIWDQQ GTMAQKGQLSDDEKFLFVDKNFINSPVAQADWA AKRLVWVPSEKQGFEAASIKEEKGDEVVVELVE NGKKVTVGKDDIQKMNPPKFSKVEDMAELTCLNE ASVLHNLRERYFSGLIYTYSGLFCVVVNPYKHL PIYSEKIVDMYKGKKRHEMPPHIYAIADTAYRS MLQDREDQSILCTGESGAGKTENTKKVIQYLAVV ASSHKGKKDTSITQGPSFAYGELEKQLLQANPI LEAFGNAKTVKNDNSSRFGKFIRINFDVTGYIV GANIETYLLEKSRAIRQARDERTFHIFYYMIAGA KEKMRSDLLLEGFNNYTFLSNGFVPIPAAQDDE MFQETVEAMAIMGFSEEEQLSILKVVSSVLQLG NIVFKKERNTDQASMPDNTAAQKVCHLMGINVTD FTRSILTPRIKVGRDVVQKAQTKEQADFAVEAL AKATYERLFRWILTRVNKALDKTHRQGASFLGI LDIAGFEIFEVNSFEQLCINYTNEKLQQLFNHTM FILEQEEYQREGIEWNFIDFGLDLQPCIELIER PNNPPGVLALLDEECWFPKATDKSFVEKLCTEQ GSHPKFQKPKQLKDKTEFSIIHYAGKVDYNASAW LTKNMDPLNDNVTSLLNASSDKFVADLWKDVDR IVGLDQMAKMTESSLPSASKTKKGMFRTVGQLY KEQLGKLMTTLRNTTPNFVRCIIPNHEKRSGKLD AFLVLEQLRCNGVLEGIRICRQGFPNRIVFQEF RQRYEILAANAIPKGFMDGKQACILMIKALELD PNLYRIGQSKIFFRTGVLAHLEEERDLKITDVIM AFQAMCRGYLARKAFAKRQQQLTAMKVIQRNCA AYLKLRNWQWWRLFTKVKPLLQVTRQEEEMQAK EDELQKTKERQQKAENELKELEQKHSQLTEEKNL LQEQLQAETELYAEAEEMRVRLAAKKQELEEIL HEMEARLEEEEDRGQQLQAERKKMAQQMLDLEE QLEEEEAARQKLQLEKVTAEAKIKKLEDEILVMD DQNNKLSKERKLLEERISDLTTNLAEEEEKAKN LTKLKNKHESMISELEVRLKKEEKSRQELEKLK RKLEGDASDFHEQIADLQAQIAELKMQLAKKEEE LQAALARLDDEIAQKNNALKKIRELEGHISDLQ EDLDSERAARNKAEKQKRDLGEELEALKTELED TLDSTATQQELRAKREQEVTVLKKALDEETRSHE AQVQEMRQKHAQAVEELTEQLEQFKRAKANLDK NKQTLEKENADLAGELRVLGQAKQEVEHKKKKL EAQVQELQSKCSDGERARAELNDKVHKLQNEVES VTGMLNEAEGKAIKLAKDVASLSSQLQDTQELL QEETRQKLNVSTKLRQLEEERNSLQDQLDEEME AKQNLERHISTLNIQLSDSKKKLQDFASTVEALE EGKKRFQKEIENLTQQYEEKAAAYDKLEKTKNR LQQELDDLVVDLDNQRQLVSNLEKKQRKFDQLL AEEKNISSKYADERDRAEAEAREKETKALSLARA LEEALEAKEELERTNKMLKAEMEDLVSSKDDVG KNVHELEKSKRALETQMEEMKTQLEELEDELQA TEDAKLRLEVNMQALKGQFERDLQARDEQNEEKR RQLQRQLHEYETELEDERKQRALAAAAKKKLEG DLKDLELQADSAIKGREEAIKQLRKLQAQMKDF QRELEDARASRDEIFATAKENEKKAKSLEADLMQ LQEDLAAAERARKQADLEKEELAEELASSLSGR NALQDEKRRLEARIAQLEEELEEEQGNMEAMSD RVRKATQQAEQLSNELATERSTAQKNESARQQLE RQNKELRSKLHEMEGAVKSKFKSTIAALEAKIA QLEEQVEQEAREKQAATKSLKQKDKKLKEILLQ VEDERKMAEQYKEQAEKGNARVKQLKRQLEEAEE ESQRINANRRKLQRELDEATESNEAMGREVNAL KSKLRRGNETSFVPSRRSGGRRVIENADGSEEE TDTRDADFNGTKASE*ATFYSFAPRQENQKPKQT NKKNPTTTQNKAKPSRLYLALSKSILKFQISQT PLTQGI*KPPPSSLGNVVKPHLYKNLKISWAWW YTPVVPATREAEPGRTLQPRTSRLQ*AIIASLHS SLGNRDPVSTTTTTTTPTTPVFKVKIEVCMM*E MRKTQQETQTHPPVLSMDCADTEFLEKHIHGNR SLAILFT*NGEKVTEMGAAGPHSQFIP*PANLSN FSRQAFENSFPLSWFPHLRHAQFTKCLL*SHEL KRRRCSHGGEQECCTVYTLTVLKNTFTNKWL*I T

Sequence NM_022844

GGGAGATTTGGACGCTCCGGCCTGGGAGGTGCGTCAGATCCGAGCTCGCCATCCAGTTTCCTCTCCACTAGTCCCCCCAGTTGGAGATCTGGGACCAACA AGGCACCATGGCGCAGAAGGGCCAACTCAGTGACGATGAGAAGTTCCTCTTTGTGGACAAAAACTTCATCAACAGCCCAGTGGCCCAGGCTGACTGGGCC GCCAAGAGACTCGTCTGGGTCCCCTCGGAGAAGCAGGGCTTCGAGGCAGCCAGCATTAAGGAGGAGAAGGGGGATGAGGTGGTTGTGGAGCTGGTGGAGA ATGGCAAGAAGGTCACGGTTGGGAAAGATGACATCCAGAAGATGAACCCACCCAAGTTCTCCAAGGTGGAGGACATGGCGGAGCTGACGTGCCTCAACGA AGCCTCCGTGCTACACAACCTGAGGGAGCGGTACTTCTCAGGGCTAATATATACGTACTCTGGCCTCTTCTGCGTGGTGGTCAACCCCTATAAACACCTG CCCATCTACTCGGAGAAGATCGTCGACATGTACAAGGGCAAGAAGAGGCACGAGATGCCGCCTCACATCTACGCCATCGCAGACACGGCCTACCGGAGCA TGCTTCAAGATCGGGAGGACCAGTCCATTCTATGCACAGGCGAGTCTGGAGCCGGGAAAACCGAAAACACCAAGAAGGTCATTCAGTACCTGGCCGTGGT GGCCTCCTCCCACAAGGGCAAGAAAGACACAAGTATCACGGGAGAGCTGGAAAAGCAGCTTCTACAAGCAAACCCGATTCTGGAGGCTTTCGGCAACGCC AAAACAGTGAAGAACGACAACTCCTCACGATTCGGCAAATTCATCCGCATCAACTTCGACGTCACGGGTTACATCGTGGGAGCCAACATTGAGACCTATC TGCTAGAAAAATCACGGGCAATTCGCCAAGCCAGAGACGAGAGGACATTCCACATCTTTTACTACATGATTGCTGGAGCCAAGGAGAAGATGAGAAGTGA CTTGCTTTTGGAGGGCTTCAACAACTACACCTTCCTCTCCAATGGCTTTGTGCCCATCCCAGCAGCCCAGGATGATGAGATGTTCCAGGAAACCGTGGAG GCCATGGCAATCATGGGTTTCAGCGAGGAGGAGCAGCTATCCATATTGAAGGTGGTATCATCGGTCCTGCAGCTTGGAAATATCGTCTTCAAGAAGGAAA GAAACACAGACCAGGCGTCCATGCCAGATAACACAGCTGCTCAGAAAGTTTGCCACCTCATGGGAATTAATGTGACAGATTTCACCAGATCCATCCTCAC TCCTCGTATCAAGGTTGGGCGAGATGTGGTACAGAAAGCTCAGACAAAAGAACAGGCTGACTTTGCTGTAGAGGCTTTGGCCAAGGCAACATATGAGCGC CTTTTCCGCTGGATACTCACCCGCGTGAACAAAGCCCTGGACAAGACCCATCGGCAAGGGGCTTCCTTCCTGGGGATCCTGGATATAGCTGGATTTGAGA TCTTTGAGGTGAACTCCTTCGAGCAGCTGTGCATCAACTACACCAACGAGAAGCTGCAGCAGCTCTTCAACCACACCATGTTCATCCTGGAGCAGGAGGA GTACCAGCGCGAGGGCATCGAGTGGAACTTCATCGACTTTGGGCTGGACCTACAGCCCTGCATCGAGCTCATCGAGCGACCGAACAACCCTCCAGGTGTG CTGGCCCTGCTGGACGAGGAATGCTGGTTCCCCAAAGCCACGGACAAGTCTTTCGTGGAGAAGCTGTGCACGGAGCAGGGCAGCCACCCCAAGTTCCAGA AGCCCAAGCAGCTCAAGGACAAGACTGAGTTCTCCATCATCCATTATGCTGGGAAGGTGGACTATAATGCGAGTGCCTGGCTGACCAAGAATATGGACCC GCTGAATGACAACGTGACTTCCCTGCTCAATGCCTCCTCCGACAAGTTTGTGGCCGACCTGTGGAAGGACGTGGACCGCATCGTGGGCCTGGACCAGATG GCCAAGATGACGGAGAGCTCGCTGCCCAGCGCCTCCAAGACCAAGAAGGGCATGTTCCGCACAGTGGGGCAGCTGTACAAGGAGCAGCTGGGCAAGCTGA TGACCACGCTACGCAACACCACGCCCAACTTCGTGCGCTGCATCATCCCCAACCACGAGAAGAGGTCCGGCAAGCTGGATGCGTTCCTGGTGCTGGAGCA GCTGCGGTGCAATGGGGTGCTGGAAGGCATTCGCATCTGCCGGCAGGGCTTCCCCAACCGGATCGTCTTCCAGGAGTTCCGCCAACGCTACGAGATCCTG GCGGCGAATGCCATCCCCAAAGGCTTCATGGACGGGAAGCAGGCCTGCATTCTCATGATCAAAGCCCTGGAACTTGACCCCAACTTATACAGGATAGGGC AGAGCAAAATCTTCTTCCGAACTGGCGTCCTGGCCCACCTAGAGGAGGAGCGAGATTTGAAGATCACCGATGTCATCATGGCCTTCCAGGCGATGTGTCG TGGCTACTTGGCCAGAAAGGCTTTTGCCAAGAGGCAGCAGCAGCTGACCGCCATGAAGGTGATTCAGAGGAACTGCGCCGCCTACCTCAAGCTGCGGAAC TGGCAGTGGTGGAGGCTTTTCACCAAAGTGAAGCCACTGCTGCAGGTGACACGGCAGGAGGAGGAGATGCAGGCCAAGGAGGATGAACTGCAGAAGACCA AGGAGCGGCAGCAGAAGGCAGAGAATGAGCTTAAGGAGCTGGAACAGAAGCACTCGCAGCTGACCGAGGAGAAGAACCTGCTACAGGAACAGCTGCAGGC AGAGACAGAGCTGTATGCAGAGGCTGAGGAGATGCGGGTGCGGCTGGCGGCCAAGAAGCAGGAGCTGGAGGAGATACTGCATGAGATGGAGGCCCGCCTG GAGGAGGAGGAAGACAGGGGCCAGCAGCTACAGGCTGAAAGGAAGAAGATGGCCCAGCAGATGCTGGACCTTGAAGAACAGCTGGAGGAGGAGGAAGCTG CCAGGCAGAAGCTGCAACTTGAGAAGGTCACGGCTGAGGCCAAGATCAAGAAACTGGAGGATGAGATCCTGGTCATGGATGATCAGAACAATAAACTATC AAAAGAACGAAAACTCCTTGAGGAGAGGATTAGTGACTTAACGACAAATCTTGCAGAAGAGGAAGAAAAGGCCAAGAATCTTACCAAGCTGAAAAACAAG CATGAATCTATGATTTCAGAACTGGAAGTGCGGCTAAAGAAGGAAGAGAAGAGCCGACAGGAGCTGGAGAAGCTGAAACGGAAGCTGGAGGGTGATGCCA GCGACTTCCACGAGCAGATCGCTGACCTCCAGGCGCAGATCGCAGAGCTCAAGATGCAGCTGGCCAAGAAGGAGGAGGAGCTGCAGGCGGCCCTGGCCAG GCTTGACGATGAAATCGCTCAGAAGAACAATGCCCTGAAGAAGATCCGGGAGCTGGAGGGCCACATCTCAGACCTCCAGGAGGACCTGGACTCAGAGCGG GCCGCCAGGAACAAGGCTGAAAAGCAGAAGCGAGACCTCGGCGAGGAGCTGGAGGCCCTAAAGACAGAGCTGGAAGACACACTGGACAGCACAGCCACTC AGCAGGAGCTCAGGGCCAAGAGGGAGCAGGAGGTGACGGTGCTGAAGAAGGCCCTGGATGAAGAGACGCGGTCCCATGAGGCTCAGGTCCAGGAGATGAG GCAGAAACACGCACAGGCGGTGGAGGAGCTCACAGAGCAGCTTGAGCAGTTCAAGAGGGCCAAGGCGAACCTAGACAAGAATAAGCAGACGCTGGAGAAA GAGAACGCAGACCTGGCCGGGGAGCTGCGGGTCCTGGGCCAGGCCAAGCAGGAGGTGGAACATAAGAAGAAGAAGCTGGAGGCGCAGGTGCAGGAGCTGC AGTCCAAGTGCAGCGATGGGGAGCGGGCCCGGGCGGAGCTCAATGACAAAGTCCACAAGCTGCAGAATGAAGTTGAGAGCGTCACAGGGATGCTTAACGA GGCCGAGGGGAAGGCCATTAAGCTGGCCAAGGACGTGGCGTCCCTCAGTTCCCAGCTCCAGGACACCCAGGAGCTGCTTCAAGAAGAAACCCGGCAGAAG CTCAACGTGTCTACGAAGCTGCGCCAGCTGGAGGAGGAGCGGAACAGCCTGCAAGACCAGCTGGACGAGGAGATGGAGGCCAAGCAGAACCTGGAGCGCC ACATCTCCACTCTCAACATCCAGCTCTCCGACTCGAAGAAGAAGCTGCAGGACTTTGCCAGCACCGTGGAAGCTCTGGAAGAGGGGAAGAAGAGGTTCCA GAAGGAGATCGAGAACCTCACCCAGCAGTACGAGGAGAAGGCGGCCGCTTATGATAAACTGGAAAAGACCAAGAACAGGCTTCAGCAGGAGCTGGACGAC CTGGTTGTTGATTTGGACAACCAGCGGCAACTCGTGTCCAACCTGGAAAAGAAGCAGAGGAAATTTGATCAGTTGTTAGCCGAGGAGAAAAACATCTCTT CCAAATACGCGGATGAGAGGGACAGAGCTGAGGCAGAAGCCAGGGAGAAGGAAACCAAGGCCCTGTCCCTGGCTCGGGCCCTTGAAGAGGCCTTGGAAGC CAAAGAGGAACTCGAGCGGACCAACAAAATGCTCAAAGCCGAAATGGAAGACCTGGTCAGCTCCAAGGATGACGTGGGCAAGAACGTCCATGAGCTGGAG AAGTCCAAGCGGGCCCTGGAGACCCAGATGGAGGAGATGAAGACGCAGCTGGAAGAGCTGGAGGACGAGCTGCAAGCCACGGAGGACGCCAAACTGCGGC TGGAAGTCAACATGCAGGCGCTCAAGGGCCAGTTCGAAAGGGATCTCCAAGCCCGGGACGAGCAGAATGAGGAGAAGAGGAGGCAACTGCAGAGACAGCT TCACGAGTATGAGACGGAACTGGAAGACGAGCGAAAGCAACGTGCCCTGGCAGCTGCAGCAAAGAAGAAGCTGGAAGGGGACCTGAAAGACCTGGAGCTT CAGGCCGACTCTGCCATCAAGGGGAGGGAGGAAGCCATCAAGCAGCTACGCAAACTGCAGGCTCAGATGAAGGACTTTCAAAGAGAGCTGGAAGATGCCC GTGCCTCCAGAGATGAGATCTTTGCCACAGCCAAAGAGAATGAGAAGAAAGCCAAGAGCTTGGAAGCAGACCTCATGCAGCTACAAGAGGACCTCGCCGC CGCTGAGAGGGCTCGCAAACAAGCGGACCTCGAGAAGGAGGAACTGGCAGAGGAGCTGGCCAGTAGCCTGTCGGGAAGGAACGCACTCCAGGACGAGAAG CGCCGCCTGGAGGCCCGGATCGCCCAGCTGGAGGAGGAGCTGGAGGAGGAGCAGGGCAACATGGAGGCCATGAGCGACCGGGTCCGCAAAGCCACACAGC AGGCCGAGCAGCTCAGCAACGAGCTGGCCACAGAGCGCAGCACGGCCCAGAAGAATGAGAGTGCCCGGCAGCAGCTCGAGCGGCAGAACAAGGAGCTCCG GAGCAAGCTCCACGAGATGGAGGGGGCCGTCAAGTCCAAGTTCAAGTCCACCATCGCGGCGCTGGAGGCCAAGATTGCACAGCTGGAGGAGCAGGTCGAG CAGGAGGCCAGAGAGAAACAGGCGGCCACCAAGTCGCTGAAGCAGAAAGACAAGAAGCTGAAGGAAATCTTGCTGCAGGTGGAGGACGAGCGCAAGATGG CCGAGCAGTACAAGGAGCAGGCAGAGAAAGGCAATGCCAGGGTCAAGCAGCTCAAGAGGCAGCTGGAGGAGGCAGAGGAGGAGTCCCAGCGCATCAACGC CAACCGCAGGAAGCTGCAGCGGGAGCTGGATGAGGCCACGGAGAGCAACGAGGCCATGGGCCGCGAGGTGAACGCACTCAAGAGCAAGCTCAGAGGGCCC CCCCCACAGGAAACTTCGCAGTGATGCACCAGGCGAGGAAACGAGACCTCTTTCGTTCCTTCTAGAAGGTCTGGAGGACGTAGAGTTATTGAAAATGCAG ATGGTTCTGAGGAGGAAACGGACACTCGAGACGCAGACTTCAATGGAACCAAGGCCAGTGAATAAGCAACTTTCTACAGTTTTGCACCACGGCAAGAAAA CCAAAAACCAAAACAAACAAACAAAAAAAACCCAACAACAACCCAGAACAAAGCAAAACCCAGCAGACTGTACTTAGCATTGTCTAAATCCATTCTCAAA TTCCAAATATCACAGACACCCCTCACACAAGGAATATAAAAACCACCACCCTCCAGCCTGGGCAACGTAGTAAAACCTCATCTATACAAGAATTTAAAAA TAAGCTGGGCGTGGTGGTACACACCTGTGGTCCCAGCTACTAGGGAGGCTGAGCCAGGAAGAACGCTCCAGCCCAGGACTTCGAGGCTGCAATGAGCTAT AATTGCATCATTGCACTCCAGCCTGGGCAACAGAGACCCTGTCTCAACCACCACCACCACCACCACCCCTACTACCCCTGTATTCAAGGTAAAAATTGAA GTTTGTATGATGTAAGAGATGAGAAAAACCCAACAGGAAACACAGACACATCCTCCAGTTCTATCAATGGATTGTGCAGACACTGAGTTTTTAGAAAAAC ATATCCACGGTAACCGGTCCCTGGCAATTCTGTTTACATGAAATGGGGAGAAAGTCACCGAAATGGGTGCCGCCGGCCCCCACTCCCAATTCATTCCCTA ACCTGCAAACCTTTCCAACTTCTCACGTCAGGCCTTTGAGAATTCTTTCCCCCTCTCCTGGTTTCCACACCTCAGACACGCACAGTTCACCAAGTGCCTT CTGTAGTCACATGAATTGAAAAGGAGACGCTGCTCCCACGGAGGGGAGCAGGAATGCTGCACTGTTTACACCCTGACTGTGCTTAAAAACACTTTCACTA ATAAATGGTTATAAATCACAA

refseq_MYH11.F1 (forward primer)
refseq_MYH11.R1 (reverse primer)
Single exon skipping

Frame 0

GRFGRSGLGGASDPSSPSSFLSTSPPSWRSGTN KAPWRRRANSVTMRSSSLWTKTSSTAQWPRLTGP PRDSSGSPRRSRASRQPALRRRRGMRWLWSWWR MARRSRLGKMTSRR*THPSSPRWRTWRS*RAST KPPCYTT*GSGTSQG*YIRTLASSAWWSTPINTC PSTRRRSSTCTRARRGTRCRLTSTPSQTRPTGA CFKIGRTSPFYAQASLEPGKPKTPRRSFSTWPW WPPPTRARKTQVSRESWKSSFYKQTRFWRLSATP KQ*RTTTPHDSANSSASTSTSRVTSWEPTLRPI C*KNHGQFAKPETRGHSTSFTT*LLEPRRR*EV TCFWRASTTTPSSPMALCPSQQPRMMRCSRKPWR PWQSWVSARRSSYPY*RWYHRSCSLEISSSRRK ETQTRRPCQITQLLRKFATSWELM*QISPDPSS LLVSRLGEMWYRKLRQKNRLTLL*RLWPRQHMSA FSAGYSPA*TKPWTRPIGKGLPSWGSWI*LDLR SLR*TPSSSCASTTPTRSCSSSSTTPCSSWSRR STSARASSGTSSTLGWTYSPASSSSSDRTTLQVC WPCWTRNAGSPKPRTSLSWRSCARSRAATPSSR SPSSSRTRLSSPSSIMLGRWTIMRVPG*PRIWT R*MTT*LPCSMPPPTSLWPTCGRTWTASWAWTRW PR*RRARCPAPPRPRRACSAQWGSCTRSSWAS* *PRYATPRPTSCAASSPTTRRGPASWMRSWCWS SCGAMGCWKAFASAGRASPTGSSSRSSANATRSW RRMPSPKASWTGSRPAFS*SKPWNLTPTYTG*G RAKSSSELASWPT*RRSEI*RSPMSSWPSRRCV VATWPERLLPRGSSS*PP*R*FRGTAPPTSSCGT GSGGGFSPK*SHCCR*HGRRRRCRPRRMNCRRP RSGSRRQRMSLRSWNRSTRS*PRRRTCYRNSCR QRQSCMQRLRRCGCGWRPRSRSWRRYCMRWRPAW RRRKTGASSYRLKGRRWPSRCWTLKNSWRRRKL PGRSCNLRRSRLRPRSRNWRMRSWSWMIRTINY QKNENSLRRGLVT*RQILQKRKKRPRILPS*KTS MNL*FQNWKCG*RRKRRADRSWRS*NGSWRVMP ATSTSRSLTSRRRSQSSRCSWPRRRRSCRRPWP GLTMKSLRRTMP*RRSGSWRATSQTSRRTWTQSG PPGTRLKSRSETSARSWRP*RQSWKTHWTAQPL SRSSGPRGSRR*RC*RRPWMKRRGPMRLRSRR* GRNTHRRWRSSQSSLSSSRGPRRT*TRISRRWRK RTQTWPGSCGSWARPSRRWNIRRRSWRRRCRSC SPSAAMGSGPGRSSMTKSTSCRMKLRASQGCLT RPRGRPLSWPRTWRPSVPSSRTPRSCFKKKPGRS STCLRSCASWRRSGTACKTSWTRRWRPSRTWSA TSPLSTSSSPTRRRSCRTLPAPWKLWKRGRRGS RRRSRTSPSSTRRRRPLMINWKRPRTGFSRSWTT WLLIWTTSGNSCPTWKRSRGNLISC*PRRKTSL PNTRMRGTELRQKPGRRKPRPCPWLGPLKRPWK PKRNSSGPTKCSKPKWKTWSAPRMTWARTSMSWR SPSGPWRPRWRR*RRSWKSWRTSCKPRRTPNCG WKSTCRRSRASSKGISKPGTSRMRRRGGNCRDS FTSMRRNWKTSESNVPWQLQQRRSWKGT*KTWSF RPTLPSRGGRKPSSSYANCRLR*RTFKESWKMP VPPEMRSLPQPKRMRRKPRAWKQTSCSYKRTSP PLRGLANKRTSRRRNWQRSWPVACREGTHSRTRS AAWRPGSPSWRRSWRRSRATWRP*ATGSAKPHS RPSSSATSWPQSAARPRRMRVPGSSSSGRTRSS GASSTRWRGPSSPSSSPPSRRWRPRLHSWRSRSS RRPERNRRPPSR*SRKTRS*RKSCCRWRTSARW PSSTRSRQRKAMPGSSSSRGSWRRQRRSPSAST PTAGSCSGSWMRPRRATRPWAAR*THSRASSEGP PHRKLRSDAPGEETRPLSFLLEGLEDVELLKMQ MVLRRKRTLETQTSMEPRPVNKQLSTVLHHGKK TKNQNKQTKKTQQQPRTKQNPADCT*HCLNPFSN SKYHRHPSHKEYKNHHPPAWAT**NLIYTRI*K *AGRGGTHLWSQLLGRLSQEERSSPGLRGCNEL *LHHCTPAWATETLSQPPPPPPPLLPLYSR*KLK FV*CKR*EKPNRKHRHILQFYQWIVQTLSF*KN ISTVTGPWQFCLHEMGRKSPKWVPPAPTPNSFP NLQTFPTSHVRPLRILSPSPGFHTSDTHSSPSAF CSHMN*KGDAAPTEGSRNAALFTP*LCLKTLSL INGYKSQ

Frame 1

GDLDAPAWEVRQIRARHPVSSPLVPPVGDLGPT RHHGAEGPTQ*R*EVPLCGQKLHQQPSGPG*LG RQETRLGPLGEAGLRGSQH*GGEGG*GGCGAGGE WQEGHGWER*HPEDEPTQVLQGGGHGGADVPQR SLRATQPEGAVLLRANIYVLWPLLRGGQPL*TP AHLLGEDRRHVQGQEEARDAASHLRHRRHGLPEH ASRSGGPVHSMHRRVWSRENRKHQEGHSVPGRG GLLPQGQERHKYHGRAGKAASTSKPDSGGFRQR QNSEERQLLTIRQIHPHQLRRHGLHRGSQH*DLS ARKITGNSPSQRREDIPHLLLHDCWSQGEDEK* LAFGGLQQLHLPLQWLCAHPSSPG**DVPGNRG GHGNHGFQRGGAAIHIEGGIIGPAAWKYRLQEGK KHRPGVHAR*HSCSESLPPHGN*CDRFHQIHPH SSYQGWARCGTESSDKRTG*LCCRGFGQGNI*A PFPLDTHPREQSPGQDPSARGFLPGDPGYSWI*D L*GELLRAAVHQLHQREAAAALQPHHVHPGAGG VPARGHRVELHRLWAGPTALHRAHRATEQPSRC AGPAGRGMLVPQSHGQVFRGEAVHGAGQPPQVPE AQAAQGQD*VLHHPLCWEGGL*CECLADQEYGP AE*QRDFPAQCLLRQVCGRPVEGRGPHRGPGPD GQDDGELAAQRLQDQEGHVPHSGAAVQGAAGQAD DHATQHHAQLRALHHPQPREEVRQAGCVPGAGA AAVQWGAGRHSHLPAGLPQPDRLPGVPPTLRDP GGECHPQRLHGREAGLHSHDQSPGT*PQLIQDRA EQNLLPNWRPGPPRGGARFEDHRCHHGLPGDVS WLLGQKGFCQEAAAADRHEGDSEELRRLPQAAE LAVVEAFHQSEATAAGDTAGGGDAGQGG*TAEDQ GAAAEGRE*A*GAGTEALAADRGEEPATGTAAG RDRAVCRG*GDAGAAGGQEAGAGGDTA*DGGPP GGGGRQGPAATG*KEEDGPADAGP*RTAGGGGSC QAEAAT*EGHG*GQDQETGG*DPGHG*SEQ*TI KRTKTP*GED**LNDKSCRRGRKGQESYQAEKQ A*IYDFRTGSAAKEGREEPTGAGEAETEAGG*CQ RLPRADR*PPGADRRAQDAAGQEGGGAAGGPGQ A*R*NRSEEQCPEEDPGAGGPHLRPPGGPGLRA GRQEQG*KAEARPRRGAGGPKDRAGRHTGQHSHS AGAQGQEGAGGDGAEEGPG*RDAVP*GSGPGDE AETRTGGGGAHRAA*AVQEGQGEPRQE*ADAGE RERRPGRGAAGPGPGQAGGGT*EEEAGGAGAGAA VQVQRWGAGPGGAQ*QSPQAAE*S*ERHRDA*R GRGEGH*AGQGRGVPQFPAPGHPGAASRRNPAE AQRVYEAAPAGGGAEQPARPAGRGDGGQAEPGAP HLHSQHPALRLEEEAAGLCQHRGSSGRGEEEVP EGDREPHPAVRGEGGRL**TGKDQEQASAGAGR PGC*FGQPAATRVQPGKEAEEI*SVVSRGEKHLF QIRG*EGQS*GRSQGEGNQGPVPGSGP*RGLGS QRGTRADQQNAQSRNGRPGQLQG*RGQERP*AG EVQAGPGDPDGGDEDAAGRAGGRAASHGGRQTAA GSQHAGAQGPVRKGSPSPGRAE*GEEEATAETA SRV*DGTGRRAKATCPGSCSKEEAGRGPERPGA SGRLCHQGEGGSHQAATQTAGSDEGLSKRAGRCP CLQR*DLCHSQRE*EESQELGSRPHAATRGPRR R*EGSQTSGPREGGTGRGAGQ*PVGKERTPGRE APPGGPDRPAGGGAGGGAGQHGGHERPGPQSHTA GRAAQQRAGHRAQHGPEE*ECPAAARAAEQGAP EQAPRDGGGRQVQVQVHHRGAGGQDCTAGGAGR AGGQRETGGHQVAEAERQEAEGNLAAGGGRAQDG RAVQGAGRERQCQGQAAQEAAGGGRGGVPAHQR QPQEAAAGAG*GHGEQRGHGPRGERTQEQAQRA PPTGNFAVMHQARKRDLFRSF*KVWRT*SY*KCR WF*GGNGHSRRRLQWNQGQ*ISNFLQFCTTARK PKTKTNKQKKPNNNPEQSKTQQTVLSIV*IHSQ IPNITDTPHTRNIKTTTLQPGQRSKTSSIQEFKN KLGVVVHTCGPSY*GG*ARKNAPAQDFEAAMSY NCIIALQPGQQRPCLNHHHHHHPYYPCIQGKN* SLYDVRDEKNPTGNTDTSSSSINGLCRH*VFRKT YPR*PVPGNSVYMKWGESHRNGCRRPPLPIHSL TCKPFQLLTSGL*EFFPPLLVSTPQTRTVHQVP SVVT*IEKETLLPRRGAGMLHCLHPDCA*KHFH* *MVINH

Frame 2

EIWTLRPGRCVRSELAIQFPLH*SPQLEIWDQQ GTMAQKGQLSDDEKFLFVDKNFINSPVAQADWA AKRLVWVPSEKQGFEAASIKEEKGDEVVVELVE NGKKVTVGKDDIQKMNPPKFSKVEDMAELTCLNE ASVLHNLRERYFSGLIYTYSGLFCVVVNPYKHL PIYSEKIVDMYKGKKRHEMPPHIYAIADTAYRS MLQDREDQSILCTGESGAGKTENTKKVIQYLAVV ASSHKGKKDTSITGELEKQLLQANPILEAFGNA KTVKNDNSSRFGKFIRINFDVTGYIVGANIETY LLEKSRAIRQARDERTFHIFYYMIAGAKEKMRSD LLLEGFNNYTFLSNGFVPIPAAQDDEMFQETVE AMAIMGFSEEEQLSILKVVSSVLQLGNIVFKKE RNTDQASMPDNTAAQKVCHLMGINVTDFTRSILT PRIKVGRDVVQKAQTKEQADFAVEALAKATYER LFRWILTRVNKALDKTHRQGASFLGILDIAGFE IFEVNSFEQLCINYTNEKLQQLFNHTMFILEQEE YQREGIEWNFIDFGLDLQPCIELIERPNNPPGV LALLDEECWFPKATDKSFVEKLCTEQGSHPKFQ KPKQLKDKTEFSIIHYAGKVDYNASAWLTKNMDP LNDNVTSLLNASSDKFVADLWKDVDRIVGLDQM AKMTESSLPSASKTKKGMFRTVGQLYKEQLGKL MTTLRNTTPNFVRCIIPNHEKRSGKLDAFLVLEQ LRCNGVLEGIRICRQGFPNRIVFQEFRQRYEIL AANAIPKGFMDGKQACILMIKALELDPNLYRIG QSKIFFRTGVLAHLEEERDLKITDVIMAFQAMCR GYLARKAFAKRQQQLTAMKVIQRNCAAYLKLRN WQWWRLFTKVKPLLQVTRQEEEMQAKEDELQKT KERQQKAENELKELEQKHSQLTEEKNLLQEQLQA ETELYAEAEEMRVRLAAKKQELEEILHEMEARL EEEEDRGQQLQAERKKMAQQMLDLEEQLEEEEA ARQKLQLEKVTAEAKIKKLEDEILVMDDQNNKLS KERKLLEERISDLTTNLAEEEEKAKNLTKLKNK HESMISELEVRLKKEEKSRQELEKLKRKLEGDA SDFHEQIADLQAQIAELKMQLAKKEEELQAALAR LDDEIAQKNNALKKIRELEGHISDLQEDLDSER AARNKAEKQKRDLGEELEALKTELEDTLDSTAT QQELRAKREQEVTVLKKALDEETRSHEAQVQEMR QKHAQAVEELTEQLEQFKRAKANLDKNKQTLEK ENADLAGELRVLGQAKQEVEHKKKKLEAQVQEL QSKCSDGERARAELNDKVHKLQNEVESVTGMLNE AEGKAIKLAKDVASLSSQLQDTQELLQEETRQK LNVSTKLRQLEEERNSLQDQLDEEMEAKQNLER HISTLNIQLSDSKKKLQDFASTVEALEEGKKRFQ KEIENLTQQYEEKAAAYDKLEKTKNRLQQELDD LVVDLDNQRQLVSNLEKKQRKFDQLLAEEKNIS SKYADERDRAEAEAREKETKALSLARALEEALEA KEELERTNKMLKAEMEDLVSSKDDVGKNVHELE KSKRALETQMEEMKTQLEELEDELQATEDAKLR LEVNMQALKGQFERDLQARDEQNEEKRRQLQRQL HEYETELEDERKQRALAAAAKKKLEGDLKDLEL QADSAIKGREEAIKQLRKLQAQMKDFQRELEDA RASRDEIFATAKENEKKAKSLEADLMQLQEDLAA AERARKQADLEKEELAEELASSLSGRNALQDEK RRLEARIAQLEEELEEEQGNMEAMSDRVRKATQ QAEQLSNELATERSTAQKNESARQQLERQNKELR SKLHEMEGAVKSKFKSTIAALEAKIAQLEEQVE QEAREKQAATKSLKQKDKKLKEILLQVEDERKM AEQYKEQAEKGNARVKQLKRQLEEAEEESQRINA NRRKLQRELDEATESNEAMGREVNALKSKLRGP PPQETSQ*CTRRGNETSFVPSRRSGGRRVIENA DGSEEETDTRDADFNGTKASE*ATFYSFAPRQEN QKPKQTNKKNPTTTQNKAKPSRLYLALSKSILK FQISQTPLTQGI*KPPPSSLGNVVKPHLYKNLK ISWAWWYTPVVPATREAEPGRTLQPRTSRLQ*AI IASLHSSLGNRDPVSTTTTTTTPTTPVFKVKIE VCMM*EMRKTQQETQTHPPVLSMDCADTEFLEK HIHGNRSLAILFT*NGEKVTEMGAAGPHSQFIP* PANLSNFSRQAFENSFPLSWFPHLRHAQFTKCL L*SHELKRRRCSHGGEQECCTVYTLTVLKNTFT NKWL*IT

Sequence NM_001040113

GGGAGATTTGGACGCTCCGGCCTGGGAGGTGCGTCAGATCCGAGCTCGCCATCCAGTTTCCTCTCCACTAGTCCCCCCAGTTGGAGATCTGGGACCAACA AGGCACCATGGCGCAGAAGGGCCAACTCAGTGACGATGAGAAGTTCCTCTTTGTGGACAAAAACTTCATCAACAGCCCAGTGGCCCAGGCTGACTGGGCC GCCAAGAGACTCGTCTGGGTCCCCTCGGAGAAGCAGGGCTTCGAGGCAGCCAGCATTAAGGAGGAGAAGGGGGATGAGGTGGTTGTGGAGCTGGTGGAGA ATGGCAAGAAGGTCACGGTTGGGAAAGATGACATCCAGAAGATGAACCCACCCAAGTTCTCCAAGGTGGAGGACATGGCGGAGCTGACGTGCCTCAACGA AGCCTCCGTGCTACACAACCTGAGGGAGCGGTACTTCTCAGGGCTAATATATACGTACTCTGGCCTCTTCTGCGTGGTGGTCAACCCCTATAAACACCTG CCCATCTACTCGGAGAAGATCGTCGACATGTACAAGGGCAAGAAGAGGCACGAGATGCCGCCTCACATCTACGCCATCGCAGACACGGCCTACCGGAGCA TGCTTCAAGATCGGGAGGACCAGTCCATTCTATGCACAGGCGAGTCTGGAGCCGGGAAAACCGAAAACACCAAGAAGGTCATTCAGTACCTGGCCGTGGT GGCCTCCTCCCACAAGGGCAAGAAAGACACAAGTATCACGCAAGGCCCATCTTTTGCCTACGGAGAGCTGGAAAAGCAGCTTCTACAAGCAAACCCGATT CTGGAGGCTTTCGGCAACGCCAAAACAGTGAAGAACGACAACTCCTCACGATTCGGCAAATTCATCCGCATCAACTTCGACGTCACGGGTTACATCGTGG GAGCCAACATTGAGACCTATCTGCTAGAAAAATCACGGGCAATTCGCCAAGCCAGAGACGAGAGGACATTCCACATCTTTTACTACATGATTGCTGGAGC CAAGGAGAAGATGAGAAGTGACTTGCTTTTGGAGGGCTTCAACAACTACACCTTCCTCTCCAATGGCTTTGTGCCCATCCCAGCAGCCCAGGATGATGAG ATGTTCCAGGAAACCGTGGAGGCCATGGCAATCATGGGTTTCAGCGAGGAGGAGCAGCTATCCATATTGAAGGTGGTATCATCGGTCCTGCAGCTTGGAA ATATCGTCTTCAAGAAGGAAAGAAACACAGACCAGGCGTCCATGCCAGATAACACAGCTGCTCAGAAAGTTTGCCACCTCATGGGAATTAATGTGACAGA TTTCACCAGATCCATCCTCACTCCTCGTATCAAGGTTGGGCGAGATGTGGTACAGAAAGCTCAGACAAAAGAACAGGCTGACTTTGCTGTAGAGGCTTTG GCCAAGGCAACATATGAGCGCCTTTTCCGCTGGATACTCACCCGCGTGAACAAAGCCCTGGACAAGACCCATCGGCAAGGGGCTTCCTTCCTGGGGATCC TGGATATAGCTGGATTTGAGATCTTTGAGGTGAACTCCTTCGAGCAGCTGTGCATCAACTACACCAACGAGAAGCTGCAGCAGCTCTTCAACCACACCAT GTTCATCCTGGAGCAGGAGGAGTACCAGCGCGAGGGCATCGAGTGGAACTTCATCGACTTTGGGCTGGACCTACAGCCCTGCATCGAGCTCATCGAGCGA CCGAACAACCCTCCAGGTGTGCTGGCCCTGCTGGACGAGGAATGCTGGTTCCCCAAAGCCACGGACAAGTCTTTCGTGGAGAAGCTGTGCACGGAGCAGG GCAGCCACCCCAAGTTCCAGAAGCCCAAGCAGCTCAAGGACAAGACTGAGTTCTCCATCATCCATTATGCTGGGAAGGTGGACTATAATGCGAGTGCCTG GCTGACCAAGAATATGGACCCGCTGAATGACAACGTGACTTCCCTGCTCAATGCCTCCTCCGACAAGTTTGTGGCCGACCTGTGGAAGGACGTGGACCGC ATCGTGGGCCTGGACCAGATGGCCAAGATGACGGAGAGCTCGCTGCCCAGCGCCTCCAAGACCAAGAAGGGCATGTTCCGCACAGTGGGGCAGCTGTACA AGGAGCAGCTGGGCAAGCTGATGACCACGCTACGCAACACCACGCCCAACTTCGTGCGCTGCATCATCCCCAACCACGAGAAGAGGTCCGGCAAGCTGGA TGCGTTCCTGGTGCTGGAGCAGCTGCGGTGCAATGGGGTGCTGGAAGGCATTCGCATCTGCCGGCAGGGCTTCCCCAACCGGATCGTCTTCCAGGAGTTC CGCCAACGCTACGAGATCCTGGCGGCGAATGCCATCCCCAAAGGCTTCATGGACGGGAAGCAGGCCTGCATTCTCATGATCAAAGCCCTGGAACTTGACC CCAACTTATACAGGATAGGGCAGAGCAAAATCTTCTTCCGAACTGGCGTCCTGGCCCACCTAGAGGAGGAGCGAGATTTGAAGATCACCGATGTCATCAT GGCCTTCCAGGCGATGTGTCGTGGCTACTTGGCCAGAAAGGCTTTTGCCAAGAGGCAGCAGCAGCTGACCGCCATGAAGGTGATTCAGAGGAACTGCGCC GCCTACCTCAAGCTGCGGAACTGGCAGTGGTGGAGGCTTTTCACCAAAGTGAAGCCACTGCTGCAGGTGACACGGCAGGAGGAGGAGATGCAGGCCAAGG AGGATGAACTGCAGAAGACCAAGGAGCGGCAGCAGAAGGCAGAGAATGAGCTTAAGGAGCTGGAACAGAAGCACTCGCAGCTGACCGAGGAGAAGAACCT GCTACAGGAACAGCTGCAGGCAGAGACAGAGCTGTATGCAGAGGCTGAGGAGATGCGGGTGCGGCTGGCGGCCAAGAAGCAGGAGCTGGAGGAGATACTG CATGAGATGGAGGCCCGCCTGGAGGAGGAGGAAGACAGGGGCCAGCAGCTACAGGCTGAAAGGAAGAAGATGGCCCAGCAGATGCTGGACCTTGAAGAAC AGCTGGAGGAGGAGGAAGCTGCCAGGCAGAAGCTGCAACTTGAGAAGGTCACGGCTGAGGCCAAGATCAAGAAACTGGAGGATGAGATCCTGGTCATGGA TGATCAGAACAATAAACTATCAAAAGAACGAAAACTCCTTGAGGAGAGGATTAGTGACTTAACGACAAATCTTGCAGAAGAGGAAGAAAAGGCCAAGAAT CTTACCAAGCTGAAAAACAAGCATGAATCTATGATTTCAGAACTGGAAGTGCGGCTAAAGAAGGAAGAGAAGAGCCGACAGGAGCTGGAGAAGCTGAAAC GGAAGCTGGAGGGTGATGCCAGCGACTTCCACGAGCAGATCGCTGACCTCCAGGCGCAGATCGCAGAGCTCAAGATGCAGCTGGCCAAGAAGGAGGAGGA GCTGCAGGCGGCCCTGGCCAGGCTTGACGATGAAATCGCTCAGAAGAACAATGCCCTGAAGAAGATCCGGGAGCTGGAGGGCCACATCTCAGACCTCCAG GAGGACCTGGACTCAGAGCGGGCCGCCAGGAACAAGGCTGAAAAGCAGAAGCGAGACCTCGGCGAGGAGCTGGAGGCCCTAAAGACAGAGCTGGAAGACA CACTGGACAGCACAGCCACTCAGCAGGAGCTCAGGGCCAAGAGGGAGCAGGAGGTGACGGTGCTGAAGAAGGCCCTGGATGAAGAGACGCGGTCCCATGA GGCTCAGGTCCAGGAGATGAGGCAGAAACACGCACAGGCGGTGGAGGAGCTCACAGAGCAGCTTGAGCAGTTCAAGAGGGCCAAGGCGAACCTAGACAAG AATAAGCAGACGCTGGAGAAAGAGAACGCAGACCTGGCCGGGGAGCTGCGGGTCCTGGGCCAGGCCAAGCAGGAGGTGGAACATAAGAAGAAGAAGCTGG AGGCGCAGGTGCAGGAGCTGCAGTCCAAGTGCAGCGATGGGGAGCGGGCCCGGGCGGAGCTCAATGACAAAGTCCACAAGCTGCAGAATGAAGTTGAGAG CGTCACAGGGATGCTTAACGAGGCCGAGGGGAAGGCCATTAAGCTGGCCAAGGACGTGGCGTCCCTCAGTTCCCAGCTCCAGGACACCCAGGAGCTGCTT CAAGAAGAAACCCGGCAGAAGCTCAACGTGTCTACGAAGCTGCGCCAGCTGGAGGAGGAGCGGAACAGCCTGCAAGACCAGCTGGACGAGGAGATGGAGG CCAAGCAGAACCTGGAGCGCCACATCTCCACTCTCAACATCCAGCTCTCCGACTCGAAGAAGAAGCTGCAGGACTTTGCCAGCACCGTGGAAGCTCTGGA AGAGGGGAAGAAGAGGTTCCAGAAGGAGATCGAGAACCTCACCCAGCAGTACGAGGAGAAGGCGGCCGCTTATGATAAACTGGAAAAGACCAAGAACAGG CTTCAGCAGGAGCTGGACGACCTGGTTGTTGATTTGGACAACCAGCGGCAACTCGTGTCCAACCTGGAAAAGAAGCAGAGGAAATTTGATCAGTTGTTAG CCGAGGAGAAAAACATCTCTTCCAAATACGCGGATGAGAGGGACAGAGCTGAGGCAGAAGCCAGGGAGAAGGAAACCAAGGCCCTGTCCCTGGCTCGGGC CCTTGAAGAGGCCTTGGAAGCCAAAGAGGAACTCGAGCGGACCAACAAAATGCTCAAAGCCGAAATGGAAGACCTGGTCAGCTCCAAGGATGACGTGGGC AAGAACGTCCATGAGCTGGAGAAGTCCAAGCGGGCCCTGGAGACCCAGATGGAGGAGATGAAGACGCAGCTGGAAGAGCTGGAGGACGAGCTGCAAGCCA CGGAGGACGCCAAACTGCGGCTGGAAGTCAACATGCAGGCGCTCAAGGGCCAGTTCGAAAGGGATCTCCAAGCCCGGGACGAGCAGAATGAGGAGAAGAG GAGGCAACTGCAGAGACAGCTTCACGAGTATGAGACGGAACTGGAAGACGAGCGAAAGCAACGTGCCCTGGCAGCTGCAGCAAAGAAGAAGCTGGAAGGG GACCTGAAAGACCTGGAGCTTCAGGCCGACTCTGCCATCAAGGGGAGGGAGGAAGCCATCAAGCAGCTACGCAAACTGCAGGCTCAGATGAAGGACTTTC AAAGAGAGCTGGAAGATGCCCGTGCCTCCAGAGATGAGATCTTTGCCACAGCCAAAGAGAATGAGAAGAAAGCCAAGAGCTTGGAAGCAGACCTCATGCA GCTACAAGAGGACCTCGCCGCCGCTGAGAGGGCTCGCAAACAAGCGGACCTCGAGAAGGAGGAACTGGCAGAGGAGCTGGCCAGTAGCCTGTCGGGAAGG AACGCACTCCAGGACGAGAAGCGCCGCCTGGAGGCCCGGATCGCCCAGCTGGAGGAGGAGCTGGAGGAGGAGCAGGGCAACATGGAGGCCATGAGCGACC GGGTCCGCAAAGCCACACAGCAGGCCGAGCAGCTCAGCAACGAGCTGGCCACAGAGCGCAGCACGGCCCAGAAGAATGAGAGTGCCCGGCAGCAGCTCGA GCGGCAGAACAAGGAGCTCCGGAGCAAGCTCCACGAGATGGAGGGGGCCGTCAAGTCCAAGTTCAAGTCCACCATCGCGGCGCTGGAGGCCAAGATTGCA CAGCTGGAGGAGCAGGTCGAGCAGGAGGCCAGAGAGAAACAGGCGGCCACCAAGTCGCTGAAGCAGAAAGACAAGAAGCTGAAGGAAATCTTGCTGCAGG TGGAGGACGAGCGCAAGATGGCCGAGCAGTACAAGGAGCAGGCAGAGAAAGGCAATGCCAGGGTCAAGCAGCTCAAGAGGCAGCTGGAGGAGGCAGAGGA GGAGTCCCAGCGCATCAACGCCAACCGCAGGAAGCTGCAGCGGGAGCTGGATGAGGCCACGGAGAGCAACGAGGCCATGGGCCGCGAGGTGAACGCACTC AAGAGCAAGCTCAGAGGGCCCCCCCCACAGGAAACTTCGCAGTGATGCACCAGGCGAGGAAACGAGACCTCTTTCGTTCCTTCTAGAAGGTCTGGAGGAC GTAGAGTTATTGAAAATGCAGATGGTTCTGAGGAGGAAACGGACACTCGAGACGCAGACTTCAATGGAACCAAGGCCAGTGAATAAGCAACTTTCTACAG TTTTGCACCACGGCAAGAAAACCAAAAACCAAAACAAACAAACAAAAAAAACCCAACAACAACCCAGAACAAAGCAAAACCCAGCAGACTGTACTTAGCA TTGTCTAAATCCATTCTCAAATTCCAAATATCACAGACACCCCTCACACAAGGAATATAAAAACCACCACCCTCCAGCCTGGGCAACGTAGTAAAACCTC ATCTATACAAGAATTTAAAAATAAGCTGGGCGTGGTGGTACACACCTGTGGTCCCAGCTACTAGGGAGGCTGAGCCAGGAAGAACGCTCCAGCCCAGGAC TTCGAGGCTGCAATGAGCTATAATTGCATCATTGCACTCCAGCCTGGGCAACAGAGACCCTGTCTCAACCACCACCACCACCACCACCCCTACTACCCCT GTATTCAAGGTAAAAATTGAAGTTTGTATGATGTAAGAGATGAGAAAAACCCAACAGGAAACACAGACACATCCTCCAGTTCTATCAATGGATTGTGCAG ACACTGAGTTTTTAGAAAAACATATCCACGGTAACCGGTCCCTGGCAATTCTGTTTACATGAAATGGGGAGAAAGTCACCGAAATGGGTGCCGCCGGCCC CCACTCCCAATTCATTCCCTAACCTGCAAACCTTTCCAACTTCTCACGTCAGGCCTTTGAGAATTCTTTCCCCCTCTCCTGGTTTCCACACCTCAGACAC GCACAGTTCACCAAGTGCCTTCTGTAGTCACATGAATTGAAAAGGAGACGCTGCTCCCACGGAGGGGAGCAGGAATGCTGCACTGTTTACACCCTGACTG TGCTTAAAAACACTTTCACTAATAAATGGTTATAAATCACAA

refseq_MYH11.F1 (forward primer)
refseq_MYH11.R1 (reverse primer)
Single exon skipping

Frame 0

GRFGRSGLGGASDPSSPSSFLSTSPPSWRSGTN KAPWRRRANSVTMRSSSLWTKTSSTAQWPRLTGP PRDSSGSPRRSRASRQPALRRRRGMRWLWSWWR MARRSRLGKMTSRR*THPSSPRWRTWRS*RAST KPPCYTT*GSGTSQG*YIRTLASSAWWSTPINTC PSTRRRSSTCTRARRGTRCRLTSTPSQTRPTGA CFKIGRTSPFYAQASLEPGKPKTPRRSFSTWPW WPPPTRARKTQVSRKAHLLPTESWKSSFYKQTRF WRLSATPKQ*RTTTPHDSANSSASTSTSRVTSW EPTLRPIC*KNHGQFAKPETRGHSTSFTT*LLE PRRR*EVTCFWRASTTTPSSPMALCPSQQPRMMR CSRKPWRPWQSWVSARRSSYPY*RWYHRSCSLE ISSSRRKETQTRRPCQITQLLRKFATSWELM*Q ISPDPSSLLVSRLGEMWYRKLRQKNRLTLL*RLW PRQHMSAFSAGYSPA*TKPWTRPIGKGLPSWGS WI*LDLRSLR*TPSSSCASTTPTRSCSSSSTTP CSSWSRRSTSARASSGTSSTLGWTYSPASSSSSD RTTLQVCWPCWTRNAGSPKPRTSLSWRSCARSR AATPSSRSPSSSRTRLSSPSSIMLGRWTIMRVP G*PRIWTR*MTT*LPCSMPPPTSLWPTCGRTWTA SWAWTRWPR*RRARCPAPPRPRRACSAQWGSCT RSSWAS**PRYATPRPTSCAASSPTTRRGPASW MRSWCWSSCGAMGCWKAFASAGRASPTGSSSRSS ANATRSWRRMPSPKASWTGSRPAFS*SKPWNLT PTYTG*GRAKSSSELASWPT*RRSEI*RSPMSS WPSRRCVVATWPERLLPRGSSS*PP*R*FRGTAP PTSSCGTGSGGGFSPK*SHCCR*HGRRRRCRPR RMNCRRPRSGSRRQRMSLRSWNRSTRS*PRRRT CYRNSCRQRQSCMQRLRRCGCGWRPRSRSWRRYC MRWRPAWRRRKTGASSYRLKGRRWPSRCWTLKN SWRRRKLPGRSCNLRRSRLRPRSRNWRMRSWSW MIRTINYQKNENSLRRGLVT*RQILQKRKKRPRI LPS*KTSMNL*FQNWKCG*RRKRRADRSWRS*N GSWRVMPATSTSRSLTSRRRSQSSRCSWPRRRR SCRRPWPGLTMKSLRRTMP*RRSGSWRATSQTSR RTWTQSGPPGTRLKSRSETSARSWRP*RQSWKT HWTAQPLSRSSGPRGSRR*RC*RRPWMKRRGPM RLRSRR*GRNTHRRWRSSQSSLSSSRGPRRT*TR ISRRWRKRTQTWPGSCGSWARPSRRWNIRRRSW RRRCRSCSPSAAMGSGPGRSSMTKSTSCRMKLR ASQGCLTRPRGRPLSWPRTWRPSVPSSRTPRSCF KKKPGRSSTCLRSCASWRRSGTACKTSWTRRWR PSRTWSATSPLSTSSSPTRRRSCRTLPAPWKLW KRGRRGSRRRSRTSPSSTRRRRPLMINWKRPRTG FSRSWTTWLLIWTTSGNSCPTWKRSRGNLISC* PRRKTSLPNTRMRGTELRQKPGRRKPRPCPWLG PLKRPWKPKRNSSGPTKCSKPKWKTWSAPRMTWA RTSMSWRSPSGPWRPRWRR*RRSWKSWRTSCKP RRTPNCGWKSTCRRSRASSKGISKPGTSRMRRR GGNCRDSFTSMRRNWKTSESNVPWQLQQRRSWKG T*KTWSFRPTLPSRGGRKPSSSYANCRLR*RTF KESWKMPVPPEMRSLPQPKRMRRKPRAWKQTSC SYKRTSPPLRGLANKRTSRRRNWQRSWPVACREG THSRTRSAAWRPGSPSWRRSWRRSRATWRP*AT GSAKPHSRPSSSATSWPQSAARPRRMRVPGSSS SGRTRSSGASSTRWRGPSSPSSSPPSRRWRPRLH SWRSRSSRRPERNRRPPSR*SRKTRS*RKSCCR WRTSARWPSSTRSRQRKAMPGSSSSRGSWRRQR RSPSASTPTAGSCSGSWMRPRRATRPWAAR*THS RASSEGPPHRKLRSDAPGEETRPLSFLLEGLED VELLKMQMVLRRKRTLETQTSMEPRPVNKQLST VLHHGKKTKNQNKQTKKTQQQPRTKQNPADCT*H CLNPFSNSKYHRHPSHKEYKNHHPPAWAT**NL IYTRI*K*AGRGGTHLWSQLLGRLSQEERSSPG LRGCNEL*LHHCTPAWATETLSQPPPPPPPLLPL YSR*KLKFV*CKR*EKPNRKHRHILQFYQWIVQ TLSF*KNISTVTGPWQFCLHEMGRKSPKWVPPA PTPNSFPNLQTFPTSHVRPLRILSPSPGFHTSDT HSSPSAFCSHMN*KGDAAPTEGSRNAALFTP*L CLKTLSLINGYKSQ

Frame 1

GDLDAPAWEVRQIRARHPVSSPLVPPVGDLGPT RHHGAEGPTQ*R*EVPLCGQKLHQQPSGPG*LG RQETRLGPLGEAGLRGSQH*GGEGG*GGCGAGGE WQEGHGWER*HPEDEPTQVLQGGGHGGADVPQR SLRATQPEGAVLLRANIYVLWPLLRGGQPL*TP AHLLGEDRRHVQGQEEARDAASHLRHRRHGLPEH ASRSGGPVHSMHRRVWSRENRKHQEGHSVPGRG GLLPQGQERHKYHARPIFCLRRAGKAASTSKPD SGGFRQRQNSEERQLLTIRQIHPHQLRRHGLHRG SQH*DLSARKITGNSPSQRREDIPHLLLHDCWS QGEDEK*LAFGGLQQLHLPLQWLCAHPSSPG** DVPGNRGGHGNHGFQRGGAAIHIEGGIIGPAAWK YRLQEGKKHRPGVHAR*HSCSESLPPHGN*CDR FHQIHPHSSYQGWARCGTESSDKRTG*LCCRGF GQGNI*APFPLDTHPREQSPGQDPSARGFLPGDP GYSWI*DL*GELLRAAVHQLHQREAAAALQPHH VHPGAGGVPARGHRVELHRLWAGPTALHRAHRA TEQPSRCAGPAGRGMLVPQSHGQVFRGEAVHGAG QPPQVPEAQAAQGQD*VLHHPLCWEGGL*CECL ADQEYGPAE*QRDFPAQCLLRQVCGRPVEGRGP HRGPGPDGQDDGELAAQRLQDQEGHVPHSGAAVQ GAAGQADDHATQHHAQLRALHHPQPREEVRQAG CVPGAGAAAVQWGAGRHSHLPAGLPQPDRLPGV PPTLRDPGGECHPQRLHGREAGLHSHDQSPGT*P QLIQDRAEQNLLPNWRPGPPRGGARFEDHRCHH GLPGDVSWLLGQKGFCQEAAAADRHEGDSEELR RLPQAAELAVVEAFHQSEATAAGDTAGGGDAGQG G*TAEDQGAAAEGRE*A*GAGTEALAADRGEEP ATGTAAGRDRAVCRG*GDAGAAGGQEAGAGGDT A*DGGPPGGGGRQGPAATG*KEEDGPADAGP*RT AGGGGSCQAEAAT*EGHG*GQDQETGG*DPGHG *SEQ*TIKRTKTP*GED**LNDKSCRRGRKGQE SYQAEKQA*IYDFRTGSAAKEGREEPTGAGEAET EAGG*CQRLPRADR*PPGADRRAQDAAGQEGGG AAGGPGQA*R*NRSEEQCPEEDPGAGGPHLRPP GGPGLRAGRQEQG*KAEARPRRGAGGPKDRAGRH TGQHSHSAGAQGQEGAGGDGAEEGPG*RDAVP* GSGPGDEAETRTGGGGAHRAA*AVQEGQGEPRQ E*ADAGERERRPGRGAAGPGPGQAGGGT*EEEAG GAGAGAAVQVQRWGAGPGGAQ*QSPQAAE*S*E RHRDA*RGRGEGH*AGQGRGVPQFPAPGHPGAA SRRNPAEAQRVYEAAPAGGGAEQPARPAGRGDGG QAEPGAPHLHSQHPALRLEEEAAGLCQHRGSSG RGEEEVPEGDREPHPAVRGEGGRL**TGKDQEQ ASAGAGRPGC*FGQPAATRVQPGKEAEEI*SVVS RGEKHLFQIRG*EGQS*GRSQGEGNQGPVPGSG P*RGLGSQRGTRADQQNAQSRNGRPGQLQG*RG QERP*AGEVQAGPGDPDGGDEDAAGRAGGRAASH GGRQTAAGSQHAGAQGPVRKGSPSPGRAE*GEE EATAETASRV*DGTGRRAKATCPGSCSKEEAGR GPERPGASGRLCHQGEGGSHQAATQTAGSDEGLS KRAGRCPCLQR*DLCHSQRE*EESQELGSRPHA ATRGPRRR*EGSQTSGPREGGTGRGAGQ*PVGK ERTPGREAPPGGPDRPAGGGAGGGAGQHGGHERP GPQSHTAGRAAQQRAGHRAQHGPEE*ECPAAAR AAEQGAPEQAPRDGGGRQVQVQVHHRGAGGQDC TAGGAGRAGGQRETGGHQVAEAERQEAEGNLAAG GGRAQDGRAVQGAGRERQCQGQAAQEAAGGGRG GVPAHQRQPQEAAAGAG*GHGEQRGHGPRGERT QEQAQRAPPTGNFAVMHQARKRDLFRSF*KVWRT *SY*KCRWF*GGNGHSRRRLQWNQGQ*ISNFLQ FCTTARKPKTKTNKQKKPNNNPEQSKTQQTVLS IV*IHSQIPNITDTPHTRNIKTTTLQPGQRSKTS SIQEFKNKLGVVVHTCGPSY*GG*ARKNAPAQD FEAAMSYNCIIALQPGQQRPCLNHHHHHHPYYP CIQGKN*SLYDVRDEKNPTGNTDTSSSSINGLCR H*VFRKTYPR*PVPGNSVYMKWGESHRNGCRRP PLPIHSLTCKPFQLLTSGL*EFFPPLLVSTPQT RTVHQVPSVVT*IEKETLLPRRGAGMLHCLHPDC A*KHFH**MVINH

Frame 2

EIWTLRPGRCVRSELAIQFPLH*SPQLEIWDQQ GTMAQKGQLSDDEKFLFVDKNFINSPVAQADWA AKRLVWVPSEKQGFEAASIKEEKGDEVVVELVE NGKKVTVGKDDIQKMNPPKFSKVEDMAELTCLNE ASVLHNLRERYFSGLIYTYSGLFCVVVNPYKHL PIYSEKIVDMYKGKKRHEMPPHIYAIADTAYRS MLQDREDQSILCTGESGAGKTENTKKVIQYLAVV ASSHKGKKDTSITQGPSFAYGELEKQLLQANPI LEAFGNAKTVKNDNSSRFGKFIRINFDVTGYIV GANIETYLLEKSRAIRQARDERTFHIFYYMIAGA KEKMRSDLLLEGFNNYTFLSNGFVPIPAAQDDE MFQETVEAMAIMGFSEEEQLSILKVVSSVLQLG NIVFKKERNTDQASMPDNTAAQKVCHLMGINVTD FTRSILTPRIKVGRDVVQKAQTKEQADFAVEAL AKATYERLFRWILTRVNKALDKTHRQGASFLGI LDIAGFEIFEVNSFEQLCINYTNEKLQQLFNHTM FILEQEEYQREGIEWNFIDFGLDLQPCIELIER PNNPPGVLALLDEECWFPKATDKSFVEKLCTEQ GSHPKFQKPKQLKDKTEFSIIHYAGKVDYNASAW LTKNMDPLNDNVTSLLNASSDKFVADLWKDVDR IVGLDQMAKMTESSLPSASKTKKGMFRTVGQLY KEQLGKLMTTLRNTTPNFVRCIIPNHEKRSGKLD AFLVLEQLRCNGVLEGIRICRQGFPNRIVFQEF RQRYEILAANAIPKGFMDGKQACILMIKALELD PNLYRIGQSKIFFRTGVLAHLEEERDLKITDVIM AFQAMCRGYLARKAFAKRQQQLTAMKVIQRNCA AYLKLRNWQWWRLFTKVKPLLQVTRQEEEMQAK EDELQKTKERQQKAENELKELEQKHSQLTEEKNL LQEQLQAETELYAEAEEMRVRLAAKKQELEEIL HEMEARLEEEEDRGQQLQAERKKMAQQMLDLEE QLEEEEAARQKLQLEKVTAEAKIKKLEDEILVMD DQNNKLSKERKLLEERISDLTTNLAEEEEKAKN LTKLKNKHESMISELEVRLKKEEKSRQELEKLK RKLEGDASDFHEQIADLQAQIAELKMQLAKKEEE LQAALARLDDEIAQKNNALKKIRELEGHISDLQ EDLDSERAARNKAEKQKRDLGEELEALKTELED TLDSTATQQELRAKREQEVTVLKKALDEETRSHE AQVQEMRQKHAQAVEELTEQLEQFKRAKANLDK NKQTLEKENADLAGELRVLGQAKQEVEHKKKKL EAQVQELQSKCSDGERARAELNDKVHKLQNEVES VTGMLNEAEGKAIKLAKDVASLSSQLQDTQELL QEETRQKLNVSTKLRQLEEERNSLQDQLDEEME AKQNLERHISTLNIQLSDSKKKLQDFASTVEALE EGKKRFQKEIENLTQQYEEKAAAYDKLEKTKNR LQQELDDLVVDLDNQRQLVSNLEKKQRKFDQLL AEEKNISSKYADERDRAEAEAREKETKALSLARA LEEALEAKEELERTNKMLKAEMEDLVSSKDDVG KNVHELEKSKRALETQMEEMKTQLEELEDELQA TEDAKLRLEVNMQALKGQFERDLQARDEQNEEKR RQLQRQLHEYETELEDERKQRALAAAAKKKLEG DLKDLELQADSAIKGREEAIKQLRKLQAQMKDF QRELEDARASRDEIFATAKENEKKAKSLEADLMQ LQEDLAAAERARKQADLEKEELAEELASSLSGR NALQDEKRRLEARIAQLEEELEEEQGNMEAMSD RVRKATQQAEQLSNELATERSTAQKNESARQQLE RQNKELRSKLHEMEGAVKSKFKSTIAALEAKIA QLEEQVEQEAREKQAATKSLKQKDKKLKEILLQ VEDERKMAEQYKEQAEKGNARVKQLKRQLEEAEE ESQRINANRRKLQRELDEATESNEAMGREVNAL KSKLRGPPPQETSQ*CTRRGNETSFVPSRRSGG RRVIENADGSEEETDTRDADFNGTKASE*ATFYS FAPRQENQKPKQTNKKNPTTTQNKAKPSRLYLA LSKSILKFQISQTPLTQGI*KPPPSSLGNVVKP HLYKNLKISWAWWYTPVVPATREAEPGRTLQPRT SRLQ*AIIASLHSSLGNRDPVSTTTTTTTPTTP VFKVKIEVCMM*EMRKTQQETQTHPPVLSMDCA DTEFLEKHIHGNRSLAILFT*NGEKVTEMGAAGP HSQFIP*PANLSNFSRQAFENSFPLSWFPHLRH AQFTKCLL*SHELKRRRCSHGGEQECCTVYTLT VLKNTFTNKWL*IT