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Sequences

Sequence NM_032485

CTTTGAGCGGAAGTTGGATCACTGAAGCGCCAAAAAAGAATTTAGGGGAAGACCTGATTTTCGCTTGAGGCATTTTTGCGGCGCTGTGCGCTACAGACAC
CTTCTGGAAGCTGCGGTGGGGAAACTGAGTTTCCCGAGCCGTTGAGACAGATGGGGTTCAGCACCGCGCGGGGACGACAGGAAAGCTGCGGCTGAAGAGA
ACTGCGGACTTGGCAGGAGTCGCCTGAGGAGCTAAGATCCCGGAGACTCTTAGCCCCGTGGTGCTTCTCTACGGGAGGGAGGGAGGGAGAATCCGGCCGG
ACAGATCTGCGCGTATCCTGGAGCCGGCCCAGTTGTGAACTAGGAGAGCTTTGGGACCTCTGTCCCAAGCAAGAGAGATGAATGGAGAGTATAGAGGCAG
AGGATTTGGACGAGGAAGATTTCAAAGCTGGAAAAGGGGAAGAGGTGGTGGGAACTTCTCAGGAAAATGGAGAGAAAGAGAACACAGACCTGATCTGAGT
AAAACCACAGGAAAACGTACTTCTGAACAAACCCCACAGTTTTTGCTTTCAACAAAGACCCCACAGTCAATGCAGTCAACATTGGATCGATTCATACCAT
ATAAAGGCTGGAAGCTTTATTTCTCTGAAGTTTACAGCGATAGCTCTCCTTTGATTGAGAAGATTCAAGCATTTGAAAAATTTTTCACAAGGCATATTGA
TTTGTATGACAAGGATGAAATAGAAAGAAAGGGAAGTATTTTGGTAGATTTTAAAGAACTGACAGAAGGTGGTGAAGTAACTAACTTGATACCAGATATA
GCAACTGAACTAAGAGATGCACCTGAGAAAACCTTGGCTTGCATGGGTTTGGCAATACATCAGGTGTTAACTAAGGACCTTGAAAGGCATGCAGCTGAGT
TACAAGCCCAGGAAGGATTGTCTAATGATGGAGAAACAATGGTAAATGTGCCACATATTCATGCAAGGGTGTACAACTATGAGCCTTTGACACAGCTCAA
GAATGTCAGAGCAAATTACTATGGAAAATACATTGCTCTAAGAGGGACAGTGGTTCGTGTCAGTAATATAAAGCCTCTTTGCACCAAGATGGCTTTTCTT
TGTGCTGCATGTGGAGAAATTCAGAGCTTTCCTCTTCCAGATGGAAAATACAGTCTTCCCACAAAGTGTCCTGTGCCTGTGTGTCGAGGCAGGTCATTTA
CTGCTCTCCGCAGCTCTCCTCTCACAGTTACGATGGACTGGCAGTCAATCAAAATCCAGGAATTGATGTCTGATGATCAGAGAGAAGCAGGTCGGATTCC
ACGAACAATAGAATGTGAGCTTGTTCATGATCTTGTGGATAGCTGTGTCCCGGGAGACACAGTGACTATTACTGGAATTGTCAAAGTCTCAAATGCGGAA
GAAGGTTCTCGAAATAAGAATGACAAGTGTATGTTCCTTTTGTATATTGAAGCAAATTCTATTAGTAATAGCAAAGGACAGAAAACAAAGAGTTCTGAGG
ATGGGTGTAAGCATGGAATGTTGATGGAGTTCTCACTTAAAGACCTTTATGCCATCCAAGAGATTCAAGCTGAAGAAAACCTGTTTAAACTCATTGTCAA
CTCGCTTTGCCCTGTCATTTTTGGTCATGAACTTGTTAAAGCAGGTTTGGCATTAGCACTCTTTGGAGGAAGCCAGAAATACGCAGATGACAAAAACAGA
ATTCCAATTCGGGGAGACCCCCACATCCTTGTTGTTGGAGATCCAGGCCTAGGAAAAAGTCAAATGCTACAGGCAGCGTGCAATGTTGCCCCACGTGGCG
TGTATGTTTGTGGTAACACCACGACCACCTCTGGTCTGACGGTAACTCTTTCAAAAGATAGTTCCTCTGGAGATTTTGCTTTGGAAGCTGGTGCCCTGGT
ACTTGGTGATCAAGGTATTTGTGGAATCGATGAATTTGATAAGATGGGGAATCAACATCAAGCCTTGTTGGAAGCCATGGAGCAGCAAAGTATTAGTCTT
GCTAAGGCTGGTGTGGTTTGTAGCCTTCCTGCAAGAACTTCCATTATTGCTGCTGCAAATCCAGTTGGAGGACATTACAATAAAGCCAAAACAGTTTCTG
AGAATTTAAAAATGGGGAGTGCACTACTATCCAGATTTGATTTGGTCTTTATCCTGTTAGATACTCCAAATGAGCATCATGATCACTTACTCTCTGAACA
TGTGATTGCAATAAGAGCTGGAAAGCAGAGAACCATTAGCAGTGCCACAGTAGCTCGTATGAATAGTCAAGATTCAAATACTTCCGTACTTGAAGTAGTT
TCTGAGAAGCCATTATCAGAAAGACTAAAGGTGGTTCCTGGAGAAACAATAGATCCCATTCCCCACCAGCTATTGAGAAAGTACATTGGCTATGCTCGGC
AGTATGTGTACCCAAGGCTATCCACAGAAGCTGCTCGAGTTCTTCAAGATTTTTACCTTGAGCTCCGGAAACAGAGCCAGAGGTTAAATAGCTCACCAAT
CACTACCAGGCAGCTGGAATCTTTGATTCGTCTGACAGAGGCACGAGCAAGGTTGGAATTGAGAGAGGAAGCAACCAAAGAAGACGCTGAGGATATAGTG
GAAATTATGAAATATAGCATGCTAGGAACTTACTCTGATGAATTTGGGAACCTAGATTTTGAGCGATCCCAGCATGGTTCTGGAATGAGCAACAGGTCAA
CAGCGAAAAGATTTATTTCTGCTCTCAACAACGTTGCTGAAAGAACTTATAATAATATATTTCAATTTCATCAACTTCGGCAGATTGCCAAAGAACTAAA
CATTCAGGTTGCTGATTTTGAAAATTTTATTGGATCACTAAATGACCAGGGTTACCTCTTGAAAAAAGGCCCAAAAGTTTACCAGCTTCAAACTATGTAA
AAGGACTTCACCAAGTTAGGGCCTCCTGGGTTTATTGCAGATTAAAGCCATCTCAGTGAAGATATGCGTGCACGCACAGACAGACAGACACACACACACA
CACACACACACACACACACACACACACACACAGTCAAATACTGTTCTCTGAAAAATGATGTCCCAAAAGTATTATAATAGGAAAAAAGCATTAAATATAA
TAAACTAATTTAAGAAGTGATAAAGTCTCCAGATGCAGTAGCTCACACTGTAATCACAGTGACTCAGGAGGCTGAGGTGAGAGGATTCCTTGAGGCCAGG
GTTCGAGACCAACCTTGGGCAACATAGCAAGACCCCATTTCTTAAAAAAAAAAAAAAAAAATTTAAACTTAGCTGGGTATGGTGGCACATGCCTATAGTC
TCAGCTACTTGTGAGGCTGAGGCAGGAGGATTCTTTGAGCCCAGGAGTTTGAGGTTACAGTGAGCCACAATCACACCAATCACTGCACTCCAGCCTGGGC
AATAAAGTAACTCTTGACTCAAAAAAATAAAAAAAATTGTAGTGGTAGCCATGTGTTAATTGTTAAATAAATTCTCCAAAGGGCTAAAAGTAAATTACTT
ATAAATTTTTTATAGTTGTATTTTTGACCTGCCTTTTATATGTATGAATATTTCATAGTTTTGCATATCAGATGTAGGCATACAGACAAATACATAAACC
AATGAATATATTACATATTCTGTGTTCCAATAAAACTTTATTTATGGACACTAAAATTTGAATTTCATAAAATTTTCCCATGTCAAGAATACAAAA
  • refseq_MCM8.F2 (forward primer)
  • refseq_MCM8.R2 (reverse primer)
  • Alternative donor

Frame 0

L*AEVGSLKRQKRI*GKT*FSLEAFLRRCALQT
PSGSCGGETEFPEPLRQMGFSTARGRQESCG*RE
LRTWQESPEELRSRRLLAPWCFSTGGREGESGR
TDLRVSWSRPSCELGELWDLCPKQER*MESIEA
EDLDEEDFKAGKGEEVVGTSQENGEKENTDLI*V
KPQENVLLNKPHSFCFQQRPHSQCSQHWIDSYH
IKAGSFISLKFTAIALL*LRRFKHLKNFSQGIL
ICMTRMK*KEREVFW*ILKN*QKVVK*LT*YQI*
QLN*EMHLRKPWLAWVWQYIRC*LRTLKGMQLS
YKPRKDCLMMEKQW*MCHIFMQGCTTMSL*HSS
RMSEQITMENTLL*EGQWFVSVI*SLFAPRWLFF
VLHVEKFRAFLFQMENTVFPQSVLCLCVEAGHL
LLSAALLSQLRWTGSQSKSRN*CLMIREKQVGF
HEQ*NVSLFMILWIAVSRETQ*LLLELSKSQMRK
KVLEIRMTSVCSFCILKQILLVIAKDRKQRVLR
MGVSMEC*WSSHLKTFMPSKRFKLKKTCLNSLS
TRFALSFLVMNLLKQVWH*HSLEEARNTQMTKTE
FQFGETPTSLLLEIQA*EKVKCYRQRAMLPHVA
CMFVVTPRPPLV*R*LFQKIVPLEILLWKLVPW
YLVIKVFVESMNLIRWGINIKPCWKPWSSKVLVL
LRLVWFVAFLQELPLLLLQIQLEDITIKPKQFL
RI*KWGVHYYPDLIWSLSC*ILQMSIMITYSLN
M*LQ*ELESREPLAVPQ*LV*IVKIQILPYLK*F
LRSHYQKD*RWFLEKQ*IPFPTSY*ESTLAMLG
SMCTQGYPQKLLEFFKIFTLSSGNRARG*IAHQ
SLPGSWNL*FV*QRHEQGWN*ERKQPKKTLRI*W
KL*NIAC*ELTLMNLGT*ILSDPSMVLE*ATGQ
QRKDLFLLSTTLLKELIIIYFNFINFGRLPKN*
TFRLLILKILLDH*MTRVTS*KKAQKFTSFKLCK
RTSPS*GLLGLLQIKAISVKICVHAQTDRHTHT
HTHTHTHTHTQSNTVL*KMMSQKYYNRKKALNI
IN*FKK**SLQMQ*LTL*SQ*LRRLR*EDSLRPG
FETNLGQHSKTPFLKKKKKKFKLSWVWWHMPIV
SATCEAEAGGFFEPRSLRLQ*ATITPITALQPG
Q*SNS*LKKIKKIVVVAMC*LLNKFSKGLKVNYL
*IFYSCIFDLPFICMNIS*FCISDVGIQTNT*T
NEYITYSVFQ*NFIYGH*NLNFIKFSHVKNTK

Frame 1

FERKLDH*SAKKEFRGRPDFRLRHFCGAVRYRH
LLEAAVGKLSFPSR*DRWGSAPRGDDRKAAAEE
NCGLGRSRLRS*DPGDS*PRGASLREGGRENPAG
QICAYPGAGPVVN*ESFGTSVPSKRDEWRV*RQ
RIWTRKISKLEKGKRWWELLRKMERKRTQT*SE
*NHRKTYF*TNPTVFAFNKDPTVNAVNIGSIHTI
*RLEALFL*SLQR*LSFD*EDSSI*KIFHKAY*
FV*QG*NRKKGKYFGRF*RTDRRW*SN*LDTRY
SN*TKRCT*ENLGLHGFGNTSGVN*GP*KACS*V
TSPGRIV**WRNNGKCATYSCKGVQL*AFDTAQ
ECQSKLLWKIHCSKRDSGSCQ*YKASLHQDGFS
LCCMWRNSELSSSRWKIQSSHKVSCACVSRQVIY
CSPQLSSHSYDGLAVNQNPGIDV**SERSRSDS
TNNRM*ACS*SCG*LCPGRHSDYYWNCQSLKCG
RRFSK*E*QVYVPFVY*SKFY***QRTENKEF*G
WV*AWNVDGVLT*RPLCHPRDSS*RKPV*THCQ
LALPCHFWS*TC*SRFGISTLWRKPEIRR*QKQ
NSNSGRPPHPCCWRSRPRKKSNATGSVQCCPTWR
VCLW*HHDHLWSDGNSFKR*FLWRFCFGSWCPG
TW*SRYLWNR*I**DGESTSSLVGSHGAAKY*S
C*GWCGL*PSCKNFHYCCCKSSWRTLQ*SQNSF*
EFKNGECTTIQI*FGLYPVRYSK*AS*SLTL*T
CDCNKSWKAENH*QCHSSSYE*SRFKYFRT*SS
F*EAIIRKTKGGSWRNNRSHSPPAIEKVHWLCSA
VCVPKAIHRSCSSSSRFLP*APETEPEVK*LTN
HYQAAGIFDSSDRGTSKVGIERGSNQRRR*GYS
GNYEI*HARNLL**IWEPRF*AIPAWFWNEQQVN
SEKIYFCSQQRC*KNL**YISISSTSADCQRTK
HSGC*F*KFYWITK*PGLPLEKRPKSLPASNYV
KGLHQVRASWVYCRLKPSQ*RYACTHRQTDTHTH
THTHTHTHTHSQILFSEK*CPKSIIIGKKH*I*
*TNLRSDKVSRCSSSHCNHSDSGG*GERIP*GQ
GSRPTLGNIARPHFLKKKKKNLNLAGYGGTCL*S
QLLVRLRQEDSLSPGV*GYSEPQSHQSLHSSLG
NKVTLDSKK*KKL*W*PCVNC*INSPKG*K*IT
YKFFIVVFLTCLLYV*IFHSFAYQM*AYRQIHKP
MNILHILCSNKTLFMDTKI*IS*NFPMSRIQ

Frame 2

LSGSWITEAPKKNLGEDLIFA*GIFAALCATDT
FWKLRWGN*VSRAVETDGVQHRAGTTGKLRLKR
TADLAGVA*GAKIPETLSPVVLLYGREGGRIRP
DRSARILEPAQL*TRRALGPLSQAREMNGEYRGR
GFGRGRFQSWKRGRGGGNFSGKWREREHRPDLS
KTTGKRTSEQTPQFLLSTKTPQSMQSTLDRFIP
YKGWKLYFSEVYSDSSPLIEKIQAFEKFFTRHID
LYDKDEIERKGSILVDFKELTEGGEVTNLIPDI
ATELRDAPEKTLACMGLAIHQVLTKDLERHAAE
LQAQEGLSNDGETMVNVPHIHARVYNYEPLTQLK
NVRANYYGKYIALRGTVVRVSNIKPLCTKMAFL
CAACGEIQSFPLPDGKYSLPTKCPVPVCRGRSF
TALRSSPLTVTMDWQSIKIQELMSDDQREAGRIP
RTIECELVHDLVDSCVPGDTVTITGIVKVSNAE
EGSRNKNDKCMFLLYIEANSISNSKGQKTKSSE
DGCKHGMLMEFSLKDLYAIQEIQAEENLFKLIVN
SLCPVIFGHELVKAGLALALFGGSQKYADDKNR
IPIRGDPHILVVGDPGLGKSQMLQAACNVAPRG
VYVCGNTTTTSGLTVTLSKDSSSGDFALEAGALV
LGDQGICGIDEFDKMGNQHQALLEAMEQQSISL
AKAGVVCSLPARTSIIAAANPVGGHYNKAKTVS
ENLKMGSALLSRFDLVFILLDTPNEHHDHLLSEH
VIAIRAGKQRTISSATVARMNSQDSNTSVLEVV
SEKPLSERLKVVPGETIDPIPHQLLRKYIGYAR
QYVYPRLSTEAARVLQDFYLELRKQSQRLNSSPI
TTRQLESLIRLTEARARLELREEATKEDAEDIV
EIMKYSMLGTYSDEFGNLDFERSQHGSGMSNRS
TAKRFISALNNVAERTYNNIFQFHQLRQIAKELN
IQVADFENFIGSLNDQGYLLKKGPKVYQLQTM*
KDFTKLGPPGFIAD*SHLSEDMRARTDRQTHTH
THTHTHTHTHTVKYCSLKNDVPKVL**EKSIKYN
KLI*EVIKSPDAVAHTVITVTQEAEVRGFLEAR
VRDQPWAT*QDPIS*KKKKKI*T*LGMVAHAYS
LSYL*G*GRRIL*AQEFEVTVSHNHTNHCTPAWA
IK*LLTQKNKKNCSGSHVLIVK*ILQRAKSKLL
INFL*LYF*PAFYMYEYFIVLHIRCRHTDKYIN
Q*IYYIFCVPIKLYLWTLKFEFHKIFPCQEYK

Sequence NM_182802

CTTTGAGCGGAAGTTGGATCACTGAAGCGCCAAAAAAGAATTTAGGGGAAGACCTGATTTTCGCTTGAGGCATTTTTGCGGCGCTGTGCGCTACAGACAC
CTTCTGGAAGCTGCGGTGGGGAAACTGAGTTTCCCGAGCCGTTGAGACAGATGGGGTTCAGCACCGCGCGGGGACGACAGGAAAGCTGCGGCTGAAGAGA
ACTGCGGACTTGGCAGGAGTCGCCTGAGGAGCTAAGATCCCGGAGACTCTTAGCCCCGTGGTGCTTCTCTACGGGAGGGAGGGAGGGAGAATCCGGCCGG
ACAGATCTGCGCGTATCCTGGAGCCGGCCCAGTTGTGAACTAGGAGAGCTTTGGGACCTCTGTCCCAAGCAAGTAAGAACTTTAGCCTTAGGAGATGTAA
ATAAAGTAGTATTTGAAGAAGCACAG
GAGAGATGAATGGAGAGTATAGAGGCAGAGGATTTGGACGAGGAAGATTTCAAAGCTGGAAAAGGGGAAGAGGT
GGTGGGAACTTCTCAGGAAAATGGAGAGAAAGAGAACACAGACCTGATCTGAGTAAAACCACAGGAAAACGTACTTCTGAACAAACCCCACAGTTTTTGC
TTTCAACAAAGACCCCACAGTCAATGCAGTCAACATTGGATCGATTCATACCATATAAAGGCTGGAAGCTTTATTTCTCTGAAGTTTACAGCGATAGCTC
TCCTTTGATTGAGAAGATTCAAGCATTTGAAAAATTTTTCACAAGGCATATTGATTTGTATGACAAGGATGAAATAGAAAGAAAGGGAAGTATTTTGGTA
GATTTTAAAGAACTGACAGAAGGTGGTGAAGTAACTAACTTGATACCAGATATAGCAACTGAACTAAGAGATGCACCTGAGAAAACCTTGGCTTGCATGG
GTTTGGCAATACATCAGGTGTTAACTAAGGACCTTGAAAGGCATGCAGCTGAGTTACAAGCCCAGGAAGGATTGTCTAATGATGGAGAAACAATGGTAAA
TGTGCCACATATTCATGCAAGGGTGTACAACTATGAGCCTTTGACACAGCTCAAGAATGTCAGAGCAAATTACTATGGAAAATACATTGCTCTAAGAGGG
ACAGTGGTTCGTGTCAGTAATATAAAGCCTCTTTGCACCAAGATGGCTTTTCTTTGTGCTGCATGTGGAGAAATTCAGAGCTTTCCTCTTCCAGATGGAA
AATACAGTCTTCCCACAAAGTGTCCTGTGCCTGTGTGTCGAGGCAGGTCATTTACTGCTCTCCGCAGCTCTCCTCTCACAGTTACGATGGACTGGCAGTC
AATCAAAATCCAGGAATTGATGTCTGATGATCAGAGAGAAGCAGGTCGGATTCCACGAACAATAGAATGTGAGCTTGTTCATGATCTTGTGGATAGCTGT
GTCCCGGGAGACACAGTGACTATTACTGGAATTGTCAAAGTCTCAAATGCGGAAGAAGCAAATTCTATTAGTAATAGCAAAGGACAGAAAACAAAGAGTT
CTGAGGATGGGTGTAAGCATGGAATGTTGATGGAGTTCTCACTTAAAGACCTTTATGCCATCCAAGAGATTCAAGCTGAAGAAAACCTGTTTAAACTCAT
TGTCAACTCGCTTTGCCCTGTCATTTTTGGTCATGAACTTGTTAAAGCAGGTTTGGCATTAGCACTCTTTGGAGGAAGCCAGAAATACGCAGATGACAAA
AACAGAATTCCAATTCGGGGAGACCCCCACATCCTTGTTGTTGGAGATCCAGGCCTAGGAAAAAGTCAAATGCTACAGGCAGCGTGCAATGTTGCCCCAC
GTGGCGTGTATGTTTGTGGTAACACCACGACCACCTCTGGTCTGACGGTAACTCTTTCAAAAGATAGTTCCTCTGGAGATTTTGCTTTGGAAGCTGGTGC
CCTGGTACTTGGTGATCAAGGTATTTGTGGAATCGATGAATTTGATAAGATGGGGAATCAACATCAAGCCTTGTTGGAAGCCATGGAGCAGCAAAGTATT
AGTCTTGCTAAGGCTGGTGTGGTTTGTAGCCTTCCTGCAAGAACTTCCATTATTGCTGCTGCAAATCCAGTTGGAGGACATTACAATAAAGCCAAAACAG
TTTCTGAGAATTTAAAAATGGGGAGTGCACTACTATCCAGATTTGATTTGGTCTTTATCCTGTTAGATACTCCAAATGAGCATCATGATCACTTACTCTC
TGAACATGTGATTGCAATAAGAGCTGGAAAGCAGAGAACCATTAGCAGTGCCACAGTAGCTCGTATGAATAGTCAAGATTCAAATACTTCCGTACTTGAA
GTAGTTTCTGAGAAGCCATTATCAGAAAGACTAAAGGTGGTTCCTGGAGAAACAATAGATCCCATTCCCCACCAGCTATTGAGAAAGTACATTGGCTATG
CTCGGCAGTATGTGTACCCAAGGCTATCCACAGAAGCTGCTCGAGTTCTTCAAGATTTTTACCTTGAGCTCCGGAAACAGAGCCAGAGGTTAAATAGCTC
ACCAATCACTACCAGGCAGCTGGAATCTTTGATTCGTCTGACAGAGGCACGAGCAAGGTTGGAATTGAGAGAGGAAGCAACCAAAGAAGACGCTGAGGAT
ATAGTGGAAATTATGAAATATAGCATGCTAGGAACTTACTCTGATGAATTTGGGAACCTAGATTTTGAGCGATCCCAGCATGGTTCTGGAATGAGCAACA
GGTCAACAGCGAAAAGATTTATTTCTGCTCTCAACAACGTTGCTGAAAGAACTTATAATAATATATTTCAATTTCATCAACTTCGGCAGATTGCCAAAGA
ACTAAACATTCAGGTTGCTGATTTTGAAAATTTTATTGGATCACTAAATGACCAGGGTTACCTCTTGAAAAAAGGCCCAAAAGTTTACCAGCTTCAAACT
ATGTAAAAGGACTTCACCAAGTTAGGGCCTCCTGGGTTTATTGCAGATTAAAGCCATCTCAGTGAAGATATGCGTGCACGCACAGACAGACAGACACACA
CACACACACACACACACACACACACACACACACACACAGTCAAATACTGTTCTCTGAAAAATGATGTCCCAAAAGTATTATAATAGGAAAAAAGCATTAA
ATATAATAAACTAATTTAAGAAGTGATAAAGTCTCCAGATGCAGTAGCTCACACTGTAATCACAGTGACTCAGGAGGCTGAGGTGAGAGGATTCCTTGAG
GCCAGGGTTCGAGACCAACCTTGGGCAACATAGCAAGACCCCATTTCTTAAAAAAAAAAAAAAAAAATTTAAACTTAGCTGGGTATGGTGGCACATGCCT
ATAGTCTCAGCTACTTGTGAGGCTGAGGCAGGAGGATTCTTTGAGCCCAGGAGTTTGAGGTTACAGTGAGCCACAATCACACCAATCACTGCACTCCAGC
CTGGGCAATAAAGTAACTCTTGACTCAAAAAAATAAAAAAAATTGTAGTGGTAGCCATGTGTTAATTGTTAAATAAATTCTCCAAAGGGCTAAAAGTAAA
TTACTTATAAATTTTTTATAGTTGTATTTTTGACCTGCCTTTTATATGTATGAATATTTCATAGTTTTGCATATCAGATGTAGGCATACAGACAAATACA
TAAACCAATGAATATATTACATATTCTGTGTTCCAATAAAACTTTATTTATGGACACTAAAATTTGAATTTCATAAAATTTTCCCATGTCAAGAATACAA
AA
  • refseq_MCM8.F2 (forward primer)
  • refseq_MCM8.R2 (reverse primer)
  • Alternative donor

Frame 0

L*AEVGSLKRQKRI*GKT*FSLEAFLRRCALQT
PSGSCGGETEFPEPLRQMGFSTARGRQESCG*RE
LRTWQESPEELRSRRLLAPWCFSTGGREGESGR
TDLRVSWSRPSCELGELWDLCPKQVRTLALGDV
NKVVFEEAQ
ER*MESIEAEDLDEEDFKAGKGEEV
VGTSQENGEKENTDLI*VKPQENVLLNKPHSFC
FQQRPHSQCSQHWIDSYHIKAGSFISLKFTAIA
LL*LRRFKHLKNFSQGILICMTRMK*KEREVFW*
ILKN*QKVVK*LT*YQI*QLN*EMHLRKPWLAW
VWQYIRC*LRTLKGMQLSYKPRKDCLMMEKQW*
MCHIFMQGCTTMSL*HSSRMSEQITMENTLL*EG
QWFVSVI*SLFAPRWLFFVLHVEKFRAFLFQME
NTVFPQSVLCLCVEAGHLLLSAALLSQLRWTGS
QSKSRN*CLMIREKQVGFHEQ*NVSLFMILWIAV
SRETQ*LLLELSKSQMRKKQILLVIAKDRKQRV
LRMGVSMEC*WSSHLKTFMPSKRFKLKKTCLNS
LSTRFALSFLVMNLLKQVWH*HSLEEARNTQMTK
TEFQFGETPTSLLLEIQA*EKVKCYRQRAMLPH
VACMFVVTPRPPLV*R*LFQKIVPLEILLWKLV
PWYLVIKVFVESMNLIRWGINIKPCWKPWSSKVL
VLLRLVWFVAFLQELPLLLLQIQLEDITIKPKQ
FLRI*KWGVHYYPDLIWSLSC*ILQMSIMITYS
LNM*LQ*ELESREPLAVPQ*LV*IVKIQILPYLK
*FLRSHYQKD*RWFLEKQ*IPFPTSY*ESTLAM
LGSMCTQGYPQKLLEFFKIFTLSSGNRARG*IA
HQSLPGSWNL*FV*QRHEQGWN*ERKQPKKTLRI
*WKL*NIAC*ELTLMNLGT*ILSDPSMVLE*AT
GQQRKDLFLLSTTLLKELIIIYFNFINFGRLPK
N*TFRLLILKILLDH*MTRVTS*KKAQKFTSFKL
CKRTSPS*GLLGLLQIKAISVKICVHAQTDRHT
HTHTHTHTHTHTQSNTVL*KMMSQKYYNRKKAL
NIIN*FKK**SLQMQ*LTL*SQ*LRRLR*EDSLR
PGFETNLGQHSKTPFLKKKKKKFKLSWVWWHMP
IVSATCEAEAGGFFEPRSLRLQ*ATITPITALQ
PGQ*SNS*LKKIKKIVVVAMC*LLNKFSKGLKVN
YL*IFYSCIFDLPFICMNIS*FCISDVGIQTNT
*TNEYITYSVFQ*NFIYGH*NLNFIKFSHVKNT
K

Frame 1

FERKLDH*SAKKEFRGRPDFRLRHFCGAVRYRH
LLEAAVGKLSFPSR*DRWGSAPRGDDRKAAAEE
NCGLGRSRLRS*DPGDS*PRGASLREGGRENPAG
QICAYPGAGPVVN*ESFGTSVPSK*EL*P*EM*
IK*YLKKHR
RDEWRV*RQRIWTRKISKLEKGKR
WWELLRKMERKRTQT*SE*NHRKTYF*TNPTVFA
FNKDPTVNAVNIGSIHTI*RLEALFL*SLQR*L
SFD*EDSSI*KIFHKAY*FV*QG*NRKKGKYFG
RF*RTDRRW*SN*LDTRYSN*TKRCT*ENLGLHG
FGNTSGVN*GP*KACS*VTSPGRIV**WRNNGK
CATYSCKGVQL*AFDTAQECQSKLLWKIHCSKR
DSGSCQ*YKASLHQDGFSLCCMWRNSELSSSRWK
IQSSHKVSCACVSRQVIYCSPQLSSHSYDGLAV
NQNPGIDV**SERSRSDSTNNRM*ACS*SCG*L
CPGRHSDYYWNCQSLKCGRSKFY***QRTENKEF
*GWV*AWNVDGVLT*RPLCHPRDSS*RKPV*TH
CQLALPCHFWS*TC*SRFGISTLWRKPEIRR*Q
KQNSNSGRPPHPCCWRSRPRKKSNATGSVQCCPT
WRVCLW*HHDHLWSDGNSFKR*FLWRFCFGSWC
PGTW*SRYLWNR*I**DGESTSSLVGSHGAAKY
*SC*GWCGL*PSCKNFHYCCCKSSWRTLQ*SQNS
F*EFKNGECTTIQI*FGLYPVRYSK*AS*SLTL
*TCDCNKSWKAENH*QCHSSSYE*SRFKYFRT*
SSF*EAIIRKTKGGSWRNNRSHSPPAIEKVHWLC
SAVCVPKAIHRSCSSSSRFLP*APETEPEVK*L
TNHYQAAGIFDSSDRGTSKVGIERGSNQRRR*G
YSGNYEI*HARNLL**IWEPRF*AIPAWFWNEQQ
VNSEKIYFCSQQRC*KNL**YISISSTSADCQR
TKHSGC*F*KFYWITK*PGLPLEKRPKSLPASN
YVKGLHQVRASWVYCRLKPSQ*RYACTHRQTDTH
THTHTHTHTHTHSQILFSEK*CPKSIIIGKKH*
I**TNLRSDKVSRCSSSHCNHSDSGG*GERIP*
GQGSRPTLGNIARPHFLKKKKKNLNLAGYGGTCL
*SQLLVRLRQEDSLSPGV*GYSEPQSHQSLHSS
LGNKVTLDSKK*KKL*W*PCVNC*INSPKG*K*
ITYKFFIVVFLTCLLYV*IFHSFAYQM*AYRQIH
KPMNILHILCSNKTLFMDTKI*IS*NFPMSRIQ

Frame 2

LSGSWITEAPKKNLGEDLIFA*GIFAALCATDT
FWKLRWGN*VSRAVETDGVQHRAGTTGKLRLKR
TADLAGVA*GAKIPETLSPVVLLYGREGGRIRP
DRSARILEPAQL*TRRALGPLSQASKNFSLRRCK
*SSI*RST
GEMNGEYRGRGFGRGRFQSWKRGRG
GGNFSGKWREREHRPDLSKTTGKRTSEQTPQFL
LSTKTPQSMQSTLDRFIPYKGWKLYFSEVYSDSS
PLIEKIQAFEKFFTRHIDLYDKDEIERKGSILV
DFKELTEGGEVTNLIPDIATELRDAPEKTLACM
GLAIHQVLTKDLERHAAELQAQEGLSNDGETMVN
VPHIHARVYNYEPLTQLKNVRANYYGKYIALRG
TVVRVSNIKPLCTKMAFLCAACGEIQSFPLPDG
KYSLPTKCPVPVCRGRSFTALRSSPLTVTMDWQS
IKIQELMSDDQREAGRIPRTIECELVHDLVDSC
VPGDTVTITGIVKVSNAEEANSISNSKGQKTKS
SEDGCKHGMLMEFSLKDLYAIQEIQAEENLFKLI
VNSLCPVIFGHELVKAGLALALFGGSQKYADDK
NRIPIRGDPHILVVGDPGLGKSQMLQAACNVAP
RGVYVCGNTTTTSGLTVTLSKDSSSGDFALEAGA
LVLGDQGICGIDEFDKMGNQHQALLEAMEQQSI
SLAKAGVVCSLPARTSIIAAANPVGGHYNKAKT
VSENLKMGSALLSRFDLVFILLDTPNEHHDHLLS
EHVIAIRAGKQRTISSATVARMNSQDSNTSVLE
VVSEKPLSERLKVVPGETIDPIPHQLLRKYIGY
ARQYVYPRLSTEAARVLQDFYLELRKQSQRLNSS
PITTRQLESLIRLTEARARLELREEATKEDAED
IVEIMKYSMLGTYSDEFGNLDFERSQHGSGMSN
RSTAKRFISALNNVAERTYNNIFQFHQLRQIAKE
LNIQVADFENFIGSLNDQGYLLKKGPKVYQLQT
M*KDFTKLGPPGFIAD*SHLSEDMRARTDRQTH
THTHTHTHTHTHTVKYCSLKNDVPKVL**EKSIK
YNKLI*EVIKSPDAVAHTVITVTQEAEVRGFLE
ARVRDQPWAT*QDPIS*KKKKKI*T*LGMVAHA
YSLSYL*G*GRRIL*AQEFEVTVSHNHTNHCTPA
WAIK*LLTQKNKKNCSGSHVLIVK*ILQRAKSK
LLINFL*LYF*PAFYMYEYFIVLHIRCRHTDKY
INQ*IYYIFCVPIKLYLWTLKFEFHKIFPCQEYK

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