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Sequences

Sequence NM_018388

AATTCATTTTTAATCCTTTAATAGTCCACAGTAATATTGTCCTAAAGAGGGTACATTGGATTTTAATTTTGCTTTCAATATGACGGCTGTCAATGTTGCC
CTGATTCGTGATACCAAGTGGCTGACTTTAGAAGTCTGTAGAGAATTTCAGAGAGGAACTTGCTCTCGAGCTGATGCAGATTGCAAGTTTGCCCATCCAC
CAAGAGTTTGCCATGTGGAAAATGGTCGTGTGGTGGCCTGTTTTGATTCTCTAAAGGGTCGGTGTACCCGAGAGAACTGCAAGTACCTTCACCCTCCTCC
ACACTTAAAAACGCAGCTGGAGATTAATGGGCGGAACAATCTGATTCAACAGAAGACTGCCGCAGCCATGTTCGCCCAGCAGATGCAGCTTATGCTCCAA
AACGCTCAAATGTCATCACTTGGTTCTTTTCCTATGACTCCATCAATTCCAGCTAATCCTCCCATGGCTTTCAATCCTTACATACCACATCCTGGGATGG
GCCTCGTTCCTGCAGAACTTGTACCAAATACACCTGTTCTGATTCCTGGAAACCCACCTCTTGCAATGCCAGGAGCTGTTGGCCCAAAACTGATGCGTTC
AGATAAACTGGAGGTTTGCCGAGAATTTCAGCGTGGAAATTGTACCCGTGGGGAGAATGATTGCCGCTATGCTCACCCTACTGATGCTTCCATGATTGAA
GCGAGTGATAATACTGTGACAATCTGCATGGATTACATCAAAGGTCGATGCTCGCGGGAGAAATGCAAGTACTTTCATCCTCCTGCACACTTGCAAGCCA
GACTCAAGGCAGCTCATCATCAGATGAACCATTCAGCTGCCTCTGCCATGGCCCTGCAGCCTGGTACACTGCAACTGATACCAAAGAGATCAGCACTGGA
AAAGCCCAATGGTGCCACCCCGGTCTTTAATCCCACTGTTTTCCACTGCCAACAGGCTCTGACTAACCTGCAGCTCCCACAGCCGGCATTTATCCCTGCA
GGGCCAATACTGTGCATGGCACCCGCTTCAAATATTGTGCCCATGATGCACGGTGCTACACCTACCACTGTGTCTGCAGCAACAACACCTGCCACCAGCG
TTCCGTTCGCTGCACCAACTACAGGCAATCAGCTGAAATTCTGAACAGCAGAGTTATGGAGTATCAGAATCTTTCCATGGAAACCTCCATATGGCCTTTC
TATATATATTCTCGTATGTCTTATTCTACCAACACAACAATAAGCGTGTTGCAGTCAATGTATTAAGCAAAGCAAACCTGCCAGCCAGCAAATTCAAATA
AAAAATAAAGCATTAAAAATCAATGGAGATGTTAAAACAACACAAATAGAAAACTAGTAACTACCATCCATCCTATTTGAATTATCAAGCAGAACATGAC
CATAAAATTTGGTAACTTGTTACATTACTCTTTGTGATTTTCTAATAACCATGCTAAGTGTATTTCCACAGTGAGCTTTTGGCTTACTATATACATTCTT
GGTGGATAAATTGTTCATCTGTTTTTGAAGTGTTACCTTACTATTTTGTTTACAAGATAGTCTATTGGGTTGATTCAGGATGTAACAAATATATTCAGTA
CCATTTCTTGTGTTGTATTGTGTTGTGCTGTGTTAGGTTTTTACATACTGTAGTGTTTTGCTGTATATGTGTGGTGTTTGATTTCAACTAAAGTGTTATT
AGTGGGGAACAGAAGTATATGTGCTTAAGAACATGACAGGTTCATGCAAATATGCTCTCTTTCTTTAGAATATTTCTGTAGGTTTCTTGGGACTGACATT
TAAAACGCCTCACTTTTGAATGTGCACAAAACCTGCTCATTAACATGCATGTGTATAATTTGTACCTGCAGATCTGATGTTGCATAATACAATCAAATTA
CTAGATTTTTTAAAGAGAGAAATAATTACCTGCACAAAGCAGAGAACTTCATAAAACATTAACCCCTAATTCACTCTTCTTAAATAGCTTGGCAAATAAG
ACTTTACCTTTAAATGAATTTCTCAGCATTTATACTAAAAATTATGTAAAGTGCTCATTAGATTTTTTGTGTGTGTGGCTTGAGAATCCCATCTCCTAAA
TTGAGTGTCTAAAACTGAGCCATTTGTCATCTTCAGCTGAGAAACTGGTACTTGGGAGCTTAAAAATATGCTAATTACAAGTTATAAATCAAACGGAGAG
ATGGGGGCATGGAGATAGTTTTTACGTACTGGAGGAAAGTGTGTAAAACCATGGCAATGTCACCTTTTACACAAATGCCATTTTCCAAATGCAAATGGCT
CATGCTCTTTAGACTACTCTTTGAATAACAAGTAAGATGCAATCTAGCAAAAGTCAGTCAGGGTGAAAGAGAATTGGTTGCAAATGAGGACTTCCCTCCC
CAAATGGACAGTCTTCTCTGTTGATCACAGAGGGAGCCTGAGTACAGGCTTGGAGAAATGGCTAGGACAGGGAACAGGGAAGCACTTACAATTATTCCTT
GATTTATTCAAAAGAACTGGGAAAGATGGTTGTAGTTGTCTTTAGCTTCGGTTCAACTGAGTTTCGTTTTGTTAAACAGTTCAGTGAAGGAGAAAGCACC
TGTGATATATGGCAAGTGTCCCCCTGCCCAAACTTTAACATCAGACCCTCTCACATCATAA
  • refseq_MBNL3.F2 (forward primer)
  • refseq_MBNL3.R2 (reverse primer)
  • Single exon skipping

Frame 0

NSFLIL**STVILS*RGYIGF*FCFQYDGCQCC
PDS*YQVADFRSL*RISERNLLSS*CRLQVCPST
KSLPCGKWSCGGLF*FSKGSVYPRELQVPSPSS
TLKNAAGD*WAEQSDSTEDCRSHVRPADAAYAP
KRSNVITWFFSYDSINSS*SSHGFQSLHTTSWDG
PRSCRTCTKYTCSDSWKPTSCNARSCWPKTDAF
R*TGGLPRISAWKLYPWGE*LPLCSPY*CFHD*
SE**YCDNLHGLHQRSMLAGEMQVLSSSCTLASQ
TQGSSSSDEPFSCLCHGPAAWYTATDTKEISTG
KAQWCHPGL*SHCFPLPTGSD*PAAPTAGIYPC
RANTVHGTRFKYCAHDARCYTYHCVCSNNTCHQR
SVRCTNYRQSAEILNSRVMEYQNLSMETSIWPF
YIYSRMSYSTNTTISVLQSMY*AKQTCQPANSN
KK*SIKNQWRC*NNTNRKLVTTIHPI*IIKQNMT
IKFGNLLHYSL*FSNNHAKCISTVSFWLTIYIL
GG*IVHLFLKCYLTILFTR*SIGLIQDVTNIFS
TISCVVLCCAVLGFYIL*CFAVYVWCLISTKVLL
VGNRSICA*EHDRFMQICSLSLEYFCRFLGTDI
*NASLLNVHKTCSLTCMCIICTCRSDVA*YNQI
TRFFKERNNYLHKAENFIKH*PLIHSS*IAWQIR
LYL*MNFSAFILKIM*SAH*IFCVCGLRIPSPK
LSV*N*AICHLQLRNWYLGA*KYANYKL*IKRR
DGGMEIVFTYWRKVCKTMAMSPFTQMPFSKCKWL
MLFRLLFE*QVRCNLAKVSQGERELVANEDFPP
QMDSLLC*SQREPEYRLGEMARTGNREALTIIP
*FIQKNWERWL*LSLASVQLSFVLLNSSVKEKAP
VIYGKCPPAQTLTSDPLTS*

Frame 1

IHF*SFNSPQ*YCPKEGTLDFNFAFNMTAVNVA
LIRDTKWLTLEVCREFQRGTCSRADADCKFAHP
PRVCHVENGRVVACFDSLKGRCTRENCKYLHPPP
HLKTQLEINGRNNLIQQKTAAAMFAQQMQLMLQ
NAQMSSLGSFPMTPSIPANPPMAFNPYIPHPGM
GLVPAELVPNTPVLIPGNPPLAMPGAVGPKLMRS
DKLEVCREFQRGNCTRGENDCRYAHPTDASMIE
ASDNTVTICMDYIKGRCSREKCKYFHPPAHLQA
RLKAAHHQMNHSAASAMALQPGTLQLIPKRSALE
KPNGATPVFNPTVFHCQQALTNLQLPQPAFIPA
GPILCMAPASNIVPMMHGATPTTVSAATTPATS
VPFAAPTTGNQLKF*TAELWSIRIFPWKPPYGLS
IYILVCLILPTQQ*ACCSQCIKQSKPASQQIQI
KNKALKINGDVKTTQIEN**LPSILFELSSRT*
P*NLVTCYITLCDFLITMLSVFPQ*AFGLLYTFL
VDKLFICF*SVTLLFCLQDSLLG*FRM*QIYSV
PFLVLYCVVLC*VFTYCSVLLYMCGV*FQLKCY
*WGTEVYVLKNMTGSCKYALFL*NISVGFLGLTF
KTPHF*MCTKPAH*HACV*FVPADLMLHNTIKL
LDFLKREIITCTKQRTS*NINP*FTLLK*LGK*
DFTFK*ISQHLY*KLCKVLIRFFVCVA*ESHLLN
*VSKTEPFVIFS*ETGTWELKNMLITSYKSNGE
MGAWR*FLRTGGKCVKPWQCHLLHKCHFPNANG
SCSLDYSLNNK*DAI*QKSVRVKENWLQMRTSLP
KWTVFSVDHRGSLSTGLEKWLGQGTGKHLQLFL
DLFKRTGKDGCSCL*LRFN*VSFC*TVQ*RRKH
L*YMASVPLPKL*HQTLSHH

Frame 2

FIFNPLIVHSNIVLKRVHWILILLSI*RLSMLP
*FVIPSG*L*KSVENFREELALELMQIASLPIH
QEFAMWKMVVWWPVLIL*RVGVPERTASTFTLL
HT*KRSWRLMGGTI*FNRRLPQPCSPSRCSLCSK
TLKCHHLVLFL*LHQFQLILPWLSILTYHILGW
ASFLQNLYQIHLF*FLETHLLQCQELLAQN*CV
QINWRFAENFSVEIVPVGRMIAAMLTLLMLP*LK
RVIIL*QSAWITSKVDARGRNASTFILLHTCKP
DSRQLIIR*TIQLPLPWPCSLVHCN*YQRDQHW
KSPMVPPRSLIPLFSTANRL*LTCSSHSRHLSLQ
GQYCAWHPLQILCP*CTVLHLPLCLQQQHLPPA
FRSLHQLQAIS*NSEQQSYGVSESFHGNLHMAF
LYIFSYVLFYQHNNKRVAVNVLSKANLPASKFK*
KIKH*KSMEMLKQHK*KTSNYHPSYLNYQAEHD
HKIW*LVTLLFVIF**PC*VYFHSELLAYYIHS
WWINCSSVFEVLPYYFVYKIVYWVDSGCNKYIQY
HFLCCIVLCCVRFLHTVVFCCICVVFDFN*SVI
SGEQKYMCLRT*QVHANMLSFFRIFL*VSWD*H
LKRLTFECAQNLLINMHVYNLYLQI*CCIIQSNY
*IF*REK*LPAQSRELHKTLTPNSLFLNSLANK
TLPLNEFLSIYTKNYVKCSLDFLCVWLENPIS*
IECLKLSHLSSSAEKLVLGSLKIC*LQVINQTER
WGHGDSFYVLEESV*NHGNVTFYTNAIFQMQMA
HAL*TTL*ITSKMQSSKSQSG*KRIGCK*GLPS
PNGQSSLLITEGA*VQAWRNG*DREQGSTYNYSL
IYSKELGKMVVVVFSFGSTEFRFVKQFSEGEST
CDIWQVSPCPNFNIRPSHII

Sequence NM_133486

AATTCATTTTTAATCCTTTAATAGTCCACAGTAATATTGTCCTAAAGAGGGTACATTGGATTTTAATTTTGCTTTCAATATGACGGCTGTCAATGTTGCC
CTGATTCGTGATACCAAGTGGCTGACTTTAGAAGTCTGTAGAGAATTTCAGAGAGGAACTTGCTCTCGAGCTGATGCAGATTGCAAGTTTGCCCATCCAC
CAAGAGTTTGCCATGTGGAAAATGGTCGTGTGGTGGCCTGTTTTGATTCTCTAAAGGGTCGGTGTACCCGAGAGAACTGCAAGTACCTTCACCCTCCTCC
ACACTTAAAAACGCAGCTGGAGATTAATGGGCGGAACAATCTGATTCAACAGAAGACTGCCGCAGCCATGTTCGCCCAGCAGATGCAGCTTATGCTCCAA
AACGCTCAAATGTCATCACTTGGTTCTTTTCCTATGACTCCATCAATTCCAGCTAATCCTCCCATGGCTTTCAATCCTTACATACCACATCCTGGGATGG
GCCTCGTTCCTGCAGAACTTGTACCAAATACACCTGTTCTGATTCCTGGAAACCCACCTCTTGCAATGCCAGGAGCTGTTGGCCCAAAACTGATGCGTTC
AGATAAACTGGAGGTTTGCCGAGAATTTCAGCGTGGAAATTGTACCCGTGGGGAGAATGATTGCCGCTATGCTCACCCTACTGATGCTTCCATGATTGAA
GCGAGTGATAATACTGTGACAATCTGCATGGATTACATCAAAGGTCGATGCTCGCGGGAGAAATGCAAGTACTTTCATCCTCCTGCACACTTGCAAGCCA
GACTCAAGGCAGCTCATCATCAGATGAACCATTCAGCTGCCTCTGCCATGGCTCTGACTAACCTGCAGCTCCCACAGCCGGCATTTATCCCTGCAGGGCC
AATACTGTGCATGGCACCCGCTTCAAATATTGTGCCCATGATGCACGGTGCTACACCTACCACTGTGTCTGCAGCAACAACACCTGCCACCAGCGTTCCG
TTCGCTGCACCAACTACAGGCAATCAGATACCCCAATTATCAATAGATGAACTGAATAGCAGCATGTTTGTTTCACAGATGTAGAAGTTCTCAGTAGAAC
ACTGAAATTCTGAACAGCAGAGTTATGGAGTATCAGAATCTTTCCATGGAAACCTCCATATGGCCTTTCTATATATATTCTCGTATGTCTTATTCTACCA
ACACAACAATAAGCGTGTTGCAGTCAATGTATTAAGCAAAGCAAACCTGCCAGCCAGCAAATTCAAATAAAAAATAAAGCATTAAAAATCAATGGAGATG
TTAAAACAACACAAATAGAAAACTAGTAACTACCATCCATCCTATTTGAATTATCAAGCAGAACATGACCATAAAATTTGGTAACTTGTTACATTACTCT
TTGTGATTTTCTAATAACCATGCTAAGTGTATTTCCACAGTGAGCTTTTGGCTTACTATATACATTCTTGGTGGATAAATTGTTCATCTGTTTTTGAAGT
GTTACCTTACTATTTTGTTTACAAGATAGTCTATTGGGTTGATTCAGGATGTAACAAATATATTCAGTACCATTTCTTGTGTTGTATTGTGTTGTGCTGT
GTTAGGTTTTTACATACTGTAGTGTTTTGCTGTATATGTGTGGTGTTTGATTTCAACTAAAGTGTTATTAGTGGGGAACAGAAGTATATGTGCTTAAGAA
CATGACAGGTTCATGCAAATATGCTCTCTTTCTTTAGAATATTTCTGTAGGTTTCTTGGGACTGACATTTAAAACGCCTCACTTTTGAATGTGCACAAAA
CCTGCTCATTAACATGCATGTGTATAATTTGTACCTGCAGATCTGATGTTGCATAATACAATCAAATTACTAGATTTTTTAAAGAGAGAAATAATTACCT
GCACAAAGCAGAGAACTTCATAAAACATTAACCCCTAATTCACTCTTCTTAAATAGCTTGGCAAATAAGACTTTACCTTTAAATGAATTTCTCAGCATTT
ATACTAAAAATTATGTAAAGTGCTCATTAGATTTTTTGTGTGTGTGGCTTGAGAATCCCATCTCCTAAATTGAGTGTCTAAAACTGAGCCATTTGTCATC
TTCAGCTGAGAAACTGGTACTTGGGAGCTTAAAAATATGCTAATTACAAGTTATAAATCAAACGGAGAGATGGGGGCATGGAGATAGTTTTTACGTACTG
GAGGAAAGTGTGTAAAACCATGGCAATGTCACCTTTTACACAAATGCCATTTTCCAAATGCAAATGGCTCATGCTCTTTAGACTACTCTTTGAATAACAA
GTAAGATGCAATCTAGCAAAAGTCAGTCAGGGTGAAAGAGAATTGGTTGCAAATGAGGACTTCCCTCCCCAAATGGACAGTCTTCTCTGTTGATCACAGA
GGGAGCCTGAGTACAGGCTTGGAGAAATGGCTAGGACAGGGAACAGGGAAGCACTTACAATTATTCCTTGATTTATTCAAAAGAACTGGGAAAGATGGTT
GTAGTTGTCTTTAGCTTCGGTTCAACTGAGTTTCGTTTTGTTAAACAGTTCAGTGAAGGAGAAAGCACCTGTGATATATGGCAAGTGTCCCCCTGCCCAA
ACTTTAACATCAGACCCTCTCACATCATAA
  • refseq_MBNL3.F2 (forward primer)
  • refseq_MBNL3.R2 (reverse primer)
  • Single exon skipping

Frame 0

NSFLIL**STVILS*RGYIGF*FCFQYDGCQCC
PDS*YQVADFRSL*RISERNLLSS*CRLQVCPST
KSLPCGKWSCGGLF*FSKGSVYPRELQVPSPSS
TLKNAAGD*WAEQSDSTEDCRSHVRPADAAYAP
KRSNVITWFFSYDSINSS*SSHGFQSLHTTSWDG
PRSCRTCTKYTCSDSWKPTSCNARSCWPKTDAF
R*TGGLPRISAWKLYPWGE*LPLCSPY*CFHD*
SE**YCDNLHGLHQRSMLAGEMQVLSSSCTLASQ
TQGSSSSDEPFSCLCHGSD*PAAPTAGIYPCRA
NTVHGTRFKYCAHDARCYTYHCVCSNNTCHQRS
VRCTNYRQSDTPIINR*TE*QHVCFTDVEVLSRT
LKF*TAELWSIRIFPWKPPYGLSIYILVCLILP
TQQ*ACCSQCIKQSKPASQQIQIKNKALKINGD
VKTTQIEN**LPSILFELSSRT*P*NLVTCYITL
CDFLITMLSVFPQ*AFGLLYTFLVDKLFICF*S
VTLLFCLQDSLLG*FRM*QIYSVPFLVLYCVVL
C*VFTYCSVLLYMCGV*FQLKCY*WGTEVYVLKN
MTGSCKYALFL*NISVGFLGLTFKTPHF*MCTK
PAH*HACV*FVPADLMLHNTIKLLDFLKREIIT
CTKQRTS*NINP*FTLLK*LGK*DFTFK*ISQHL
Y*KLCKVLIRFFVCVA*ESHLLN*VSKTEPFVI
FS*ETGTWELKNMLITSYKSNGEMGAWR*FLRT
GGKCVKPWQCHLLHKCHFPNANGSCSLDYSLNNK
*DAI*QKSVRVKENWLQMRTSLPKWTVFSVDHR
GSLSTGLEKWLGQGTGKHLQLFLDLFKRTGKDG
CSCL*LRFN*VSFC*TVQ*RRKHL*YMASVPLPK
L*HQTLSHH

Frame 1

IHF*SFNSPQ*YCPKEGTLDFNFAFNMTAVNVA
LIRDTKWLTLEVCREFQRGTCSRADADCKFAHP
PRVCHVENGRVVACFDSLKGRCTRENCKYLHPPP
HLKTQLEINGRNNLIQQKTAAAMFAQQMQLMLQ
NAQMSSLGSFPMTPSIPANPPMAFNPYIPHPGM
GLVPAELVPNTPVLIPGNPPLAMPGAVGPKLMRS
DKLEVCREFQRGNCTRGENDCRYAHPTDASMIE
ASDNTVTICMDYIKGRCSREKCKYFHPPAHLQA
RLKAAHHQMNHSAASAMALTNLQLPQPAFIPAGP
ILCMAPASNIVPMMHGATPTTVSAATTPATSVP
FAAPTTGNQIPQLSIDELNSSMFVSQM*KFSVE
H*NSEQQSYGVSESFHGNLHMAFLYIFSYVLFYQ
HNNKRVAVNVLSKANLPASKFK*KIKH*KSMEM
LKQHK*KTSNYHPSYLNYQAEHDHKIW*LVTLL
FVIF**PC*VYFHSELLAYYIHSWWINCSSVFEV
LPYYFVYKIVYWVDSGCNKYIQYHFLCCIVLCC
VRFLHTVVFCCICVVFDFN*SVISGEQKYMCLR
T*QVHANMLSFFRIFL*VSWD*HLKRLTFECAQN
LLINMHVYNLYLQI*CCIIQSNY*IF*REK*LP
AQSRELHKTLTPNSLFLNSLANKTLPLNEFLSI
YTKNYVKCSLDFLCVWLENPIS*IECLKLSHLSS
SAEKLVLGSLKIC*LQVINQTERWGHGDSFYVL
EESV*NHGNVTFYTNAIFQMQMAHAL*TTL*IT
SKMQSSKSQSG*KRIGCK*GLPSPNGQSSLLITE
GA*VQAWRNG*DREQGSTYNYSLIYSKELGKMV
VVVFSFGSTEFRFVKQFSEGESTCDIWQVSPCP
NFNIRPSHII

Frame 2

FIFNPLIVHSNIVLKRVHWILILLSI*RLSMLP
*FVIPSG*L*KSVENFREELALELMQIASLPIH
QEFAMWKMVVWWPVLIL*RVGVPERTASTFTLL
HT*KRSWRLMGGTI*FNRRLPQPCSPSRCSLCSK
TLKCHHLVLFL*LHQFQLILPWLSILTYHILGW
ASFLQNLYQIHLF*FLETHLLQCQELLAQN*CV
QINWRFAENFSVEIVPVGRMIAAMLTLLMLP*LK
RVIIL*QSAWITSKVDARGRNASTFILLHTCKP
DSRQLIIR*TIQLPLPWL*LTCSSHSRHLSLQG
QYCAWHPLQILCP*CTVLHLPLCLQQQHLPPAFR
SLHQLQAIRYPNYQ*MN*IAACLFHRCRSSQ*N
TEILNSRVMEYQNLSMETSIWPFYIYSRMSYST
NTTISVLQSMY*AKQTCQPANSNKK*SIKNQWRC
*NNTNRKLVTTIHPI*IIKQNMTIKFGNLLHYS
L*FSNNHAKCISTVSFWLTIYILGG*IVHLFLK
CYLTILFTR*SIGLIQDVTNIFSTISCVVLCCAV
LGFYIL*CFAVYVWCLISTKVLLVGNRSICA*E
HDRFMQICSLSLEYFCRFLGTDI*NASLLNVHK
TCSLTCMCIICTCRSDVA*YNQITRFFKERNNYL
HKAENFIKH*PLIHSS*IAWQIRLYL*MNFSAF
ILKIM*SAH*IFCVCGLRIPSPKLSV*N*AICH
LQLRNWYLGA*KYANYKL*IKRRDGGMEIVFTYW
RKVCKTMAMSPFTQMPFSKCKWLMLFRLLFE*Q
VRCNLAKVSQGERELVANEDFPPQMDSLLC*SQ
REPEYRLGEMARTGNREALTIIP*FIQKNWERWL
*LSLASVQLSFVLLNSSVKEKAPVIYGKCPPAQ
TLTSDPLTS*

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