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Sequences

Sequence NM_172163

AGCCATGCGTCTCTGAGCGCCCCGAGCGCGCCCCCGCCCCGGACCGTGCCCGGGCCCCGGCGCCCCCAGCCCGGCGCCGCCCATGGCCGAGGCCCCCCCG CGCCGCCTCGGCCTGGGTCCCCCGCCCGGGGACGCCCCCCGCGCGGAGCTAGTGGCGCTCACGGCCGTGCAGAGCGAACAGGGCGAGGCGGGCGGGGGCG GCTCCCCGCGCCGCCTCGGCCTCCTGGGCAGCCCCCTGCCGCCGGGCGCGCCCCTCCCTGGGCCGGGCTCCGGCTCGGGCTCCGCCTGCGGCCAGCGCTC CTCGGCCGCGCACAAGCGCTACCGCCGCCTGCAGAACTGGGTCTACAACGTGCTGGAGCGGCCCCGCGGCTGGGCCTTCGTCTACCACGTCTTCATATTT TTGCTGGTCTTCAGCTGCCTGGTGCTGTCTGTGCTGTCCACTATCCAGGAGCACCAGGAACTTGCCAACGAGTGTCTCCTCATCTTGGAATTCGTGATGA TCGTGGTTTTCGGCTTGGAGTACATCGTCCGGGTCTGGTCCGCCGGATGCTGCTGCCGCTACCGAGGATGGCAGGGTCGCTTCCGCTTTGCCAGAAAGCC CTTCTGTGTCATCGACTTCATCGTGTTCGTGGCCTCGGTGGCCGTCATCGCCGCGGGTACCCAGGGCAACATCTTCGCCACGTCCGCGCTGCGCAGCATG CGCTTCCTGCAGATCCTGCGCATGGTGCGCATGGACCGCCGCGGCGGCACCTGGAAGCTGCTGGGCTCAGTGGTCTACGCGCATAGCAAGGAGCTGATCA CCGCCTGGTACATCGGGTTCCTGGTGCTCATCTTCGCCTCCTTCCTGGTCTACCTGGCTGAGAAGGACGCCAACTCCGACTTCTCCTCCTACGCCGACTC GCTCTGGTGGGGGACGATTACATTGACAACCATCGGCTATGGTGACAAGACACCGCACACATGGCTGGGCAGGGTCCTGGCTGCTGGCTTCGCCTTACTG GGCATCTCTTTCTTTGCCCTGCCTGCCGGCATCCTAGGCTCCGGCTTTGCCCTGAAGGTCCAGGAGCAGCACCGGCAGAAGCACTTCGAGAAGCGGAGGA TGCCGGCAGCCAACCTCATCCAGGCTGCCTGGCGCCTGTACTCCACCGATATGAGCCGGGCCTACCTGACAGCCACCTGGTACTACTATGACAGTATCCT CCCATCCTTCAGCAGCCGGATGGGCATCAAAGACCGCATCCGCATGGGCAGCTCCCAGCGGCGGACGGGTCCTTCCAAGCAGCATCTGGCACCTCCAACA ATGCCCACCTCCCCAAGCAGCGAGCAGGTGGGTGAGGCCACCAGCCCCACCAAGGTGCAAAAGAGCTGGAGCTTCAATGACCGCACCCGCTTCCGGGCAT CTCTGAGACTCAAACCCCGCACCTCTGCTGAGGATGCCCCCTCAGAGGAAGTAGCAGAGGAGAAGAGCTACCAGTGTGAGCTCACGGTGGACGACATCAT GCCTGCTGTGAAGACAGTCATCCGCTCCATCAGGATTCTCAAGTTCCTGGTGGCCAAAAGGAAATTCAAGGAGACACTGCGACCGTACGACGTGAAGGAC GTCATTGAGCAGTACTCAGCAGGCCACCTGGACATGCTGGGCCGGATCAAGAGCCTGCAAACTCGGGTGGACCAAATTGTGGGTCGGGGGCCCGGGGACA GGAAGGCCCGGGAGAAGGGCGACAAGGGGCCCTCCGACGCGGAGGTGGTGGATGAAATCAGCATGATGGGACGCGTGGTCAAGGTGGAGAAGCAGGTGCA GTCCATCGAGCACAAGCTGGACCTGCTGTTGGGCTTCTATTCGCGCTGCCTGCGCTCTGGCACCTCGGCCAGCCTGGGCGCCGTGCAAGTGCCGCTGTTC GACCCCGACATCACCTCCGACTACCACAGCCCTGTGGACCACGAGGACATCTCCGTCTCCGCACAGACGCTCAGCATCTCCCGCTCGGTCAGCACCAACA TGGACTGAGGGACTTCTCAGAGGCAGGGCAGCACACGGCCAGCCCCGCGGCCTGGCGCTCCGACTGCCCTCTGAGGCCTCCGGACTCCTCTCGTACTTGA ACTCACTCCCTCACGGGGAGAGAGACCACACGCAGTATTGAGCTGCCTGAGTGGGCGTGGTACCTGCTGTGGG

refseq_KCNQ4.F1 (forward primer)
refseq_KCNQ4.R1 (reverse primer)
Single exon skipping

Frame 0

SHASLSAPSAPPPRTVPGPRRPQPGAAHGRGPP APPRPGSPARGRPPRGASGAHGRAERTGRGGRGR LPAPPRPPGQPPAAGRAPPWAGLRLGLRLRPAL LGRAQALPPPAELGLQRAGAAPRLGLRLPRLHI FAGLQLPGAVCAVHYPGAPGTCQRVSPHLGIRDD RGFRLGVHRPGLVRRMLLPLPRMAGSLPLCQKA LLCHRLHRVRGLGGRHRRGYPGQHLRHVRAAQH ALPADPAHGAHGPPRRHLEAAGLSGLRA*QGADH RLVHRVPGAHLRLLPGLPG*EGRQLRLLLLRRL ALVGDDYIDNHRLW*QDTAHMAGQGPGCWLRLT GHLFLCPACRHPRLRLCPEGPGAAPAEALREAED AGSQPHPGCLAPVLHRYEPGLPDSHLVLL*QYP PILQQPDGHQRPHPHGQLPAADGSFQAASGTSN NAHLPKQRAGG*GHQPHQGAKELELQ*PHPLPGI SETQTPHLC*GCPLRGSSRGEELPV*AHGGRHH ACCEDSHPLHQDSQVPGGQKEIQGDTATVRREG RH*AVLSRPPGHAGPDQEPANSGGPNCGSGARGQ EGPGEGRQGALRRGGGG*NQHDGTRGQGGEAGA VHRAQAGPAVGLLFALPALWHLGQPGRRASAAV RPRHHLRLPQPCGPRGHLRLRTDAQHLPLGQHQH GLRDFSEAGQHTASPAAWRSDCPLRPPDSSRT* THSLTGRETTRSIELPEWAWYLLW

Frame 1

AMRL*APRARPRPGPCPGPGAPSPAPPMAEAPP RRLGLGPPPGDAPRAELVALTAVQSEQGEAGGG GSPRRLGLLGSPLPPGAPLPGPGSGSGSACGQRS SAAHKRYRRLQNWVYNVLERPRGWAFVYHVFIF LLVFSCLVLSVLSTIQEHQELANECLLILEFVM IVVFGLEYIVRVWSAGCCCRYRGWQGRFRFARKP FCVIDFIVFVASVAVIAAGTQGNIFATSALRSM RFLQILRMVRMDRRGGTWKLLGSVVYAHSKELI TAWYIGFLVLIFASFLVYLAEKDANSDFSSYADS LWWGTITLTTIGYGDKTPHTWLGRVLAAGFALL GISFFALPAGILGSGFALKVQEQHRQKHFEKRR MPAANLIQAAWRLYSTDMSRAYLTATWYYYDSIL PSFSSRMGIKDRIRMGSSQRRTGPSKQHLAPPT MPTSPSSEQVGEATSPTKVQKSWSFNDRTRFRA SLRLKPRTSAEDAPSEEVAEEKSYQCELTVDDIM PAVKTVIRSIRILKFLVAKRKFKETLRPYDVKD VIEQYSAGHLDMLGRIKSLQTRVDQIVGRGPGD RKAREKGDKGPSDAEVVDEISMMGRVVKVEKQVQ SIEHKLDLLLGFYSRCLRSGTSASLGAVQVPLF DPDITSDYHSPVDHEDISVSAQTLSISRSVSTN MD*GTSQRQGSTRPAPRPGAPTAL*GLRTPLVLE LTPSRGERPHAVLSCLSGRGTCCG

Frame 2

PCVSERPERAPAPDRARAPAPPARRRPWPRPPR AASAWVPRPGTPPARS*WRSRPCRANRARRAGA APRAASASWAAPCRRARPSLGRAPARAPPAASA PRPRTSATAACRTGSTTCWSGPAAGPSSTTSSYF CWSSAAWCCLCCPLSRSTRNLPTSVSSSWNS** SWFSAWSTSSGSGPPDAAAATEDGRVASALPES PSVSSTSSCSWPRWPSSPRVPRATSSPRPRCAAC ASCRSCAWCAWTAAAAPGSCWAQWSTRIARS*S PPGTSGSWCSSSPPSWSTWLRRTPTPTSPPTPT RSGGGRLH*QPSAMVTRHRTHGWAGSWLLASPYW ASLSLPCLPAS*APALP*RSRSSTGRSTSRSGG CRQPTSSRLPGACTPPI*AGPT*QPPGTTMTVS SHPSAAGWASKTASAWAAPSGGRVLPSSIWHLQQ CPPPQAASRWVRPPAPPRCKRAGASMTAPASGH L*DSNPAPLLRMPPQRK*QRRRATSVSSRWTTS CLL*RQSSAPSGFSSSWWPKGNSRRHCDRTT*RT SLSSTQQATWTCWAGSRACKLGWTKLWVGGPGT GRPGRRATRGPPTRRWWMKSA*WDAWSRWRSRC SPSSTSWTCCWASIRAACALAPRPAWAPCKCRCS TPTSPPTTTALWTTRTSPSPHRRSASPARSAPT WTEGLLRGRAAHGQPRGLALRLPSEASGLLSYL NSLPHGERDHTQY*AA*VGVVPAV

Sequence NM_004700

AGCCATGCGTCTCTGAGCGCCCCGAGCGCGCCCCCGCCCCGGACCGTGCCCGGGCCCCGGCGCCCCCAGCCCGGCGCCGCCCATGGCCGAGGCCCCCCCG CGCCGCCTCGGCCTGGGTCCCCCGCCCGGGGACGCCCCCCGCGCGGAGCTAGTGGCGCTCACGGCCGTGCAGAGCGAACAGGGCGAGGCGGGCGGGGGCG GCTCCCCGCGCCGCCTCGGCCTCCTGGGCAGCCCCCTGCCGCCGGGCGCGCCCCTCCCTGGGCCGGGCTCCGGCTCGGGCTCCGCCTGCGGCCAGCGCTC CTCGGCCGCGCACAAGCGCTACCGCCGCCTGCAGAACTGGGTCTACAACGTGCTGGAGCGGCCCCGCGGCTGGGCCTTCGTCTACCACGTCTTCATATTT TTGCTGGTCTTCAGCTGCCTGGTGCTGTCTGTGCTGTCCACTATCCAGGAGCACCAGGAACTTGCCAACGAGTGTCTCCTCATCTTGGAATTCGTGATGA TCGTGGTTTTCGGCTTGGAGTACATCGTCCGGGTCTGGTCCGCCGGATGCTGCTGCCGCTACCGAGGATGGCAGGGTCGCTTCCGCTTTGCCAGAAAGCC CTTCTGTGTCATCGACTTCATCGTGTTCGTGGCCTCGGTGGCCGTCATCGCCGCGGGTACCCAGGGCAACATCTTCGCCACGTCCGCGCTGCGCAGCATG CGCTTCCTGCAGATCCTGCGCATGGTGCGCATGGACCGCCGCGGCGGCACCTGGAAGCTGCTGGGCTCAGTGGTCTACGCGCATAGCAAGGAGCTGATCA CCGCCTGGTACATCGGGTTCCTGGTGCTCATCTTCGCCTCCTTCCTGGTCTACCTGGCTGAGAAGGACGCCAACTCCGACTTCTCCTCCTACGCCGACTC GCTCTGGTGGGGGACGATTACATTGACAACCATCGGCTATGGTGACAAGACACCGCACACATGGCTGGGCAGGGTCCTGGCTGCTGGCTTCGCCTTACTG GGCATCTCTTTCTTTGCCCTGCCTGCCGGCATCCTAGGCTCCGGCTTTGCCCTGAAGGTCCAGGAGCAGCACCGGCAGAAGCACTTCGAGAAGCGGAGGA TGCCGGCAGCCAACCTCATCCAGGCTGCCTGGCGCCTGTACTCCACCGATATGAGCCGGGCCTACCTGACAGCCACCTGGTACTACTATGACAGTATCCT CCCATCCTTCAGAGAGCTGGCCCTCTTGTTTGAGCACGTGCAACGGGCCCGCAATGGGGGCCTACGGCCCCTGGAGGTGCGGCGGGCGCCGGTACCCGAC GGAGCACCCTCCCGTTACCCGCCCGTTGCCACCTGCCACCGGCCGGGCAGCACCTCCTTCTGCCCTGGGGAAAGCAGCCGGATGGGCATCAAAGACCGCA TCCGCATGGGCAGCTCCCAGCGGCGGACGGGTCCTTCCAAGCAGCATCTGGCACCTCCAACAATGCCCACCTCCCCAAGCAGCGAGCAGGTGGGTGAGGC CACCAGCCCCACCAAGGTGCAAAAGAGCTGGAGCTTCAATGACCGCACCCGCTTCCGGGCATCTCTGAGACTCAAACCCCGCACCTCTGCTGAGGATGCC CCCTCAGAGGAAGTAGCAGAGGAGAAGAGCTACCAGTGTGAGCTCACGGTGGACGACATCATGCCTGCTGTGAAGACAGTCATCCGCTCCATCAGGATTC TCAAGTTCCTGGTGGCCAAAAGGAAATTCAAGGAGACACTGCGACCGTACGACGTGAAGGACGTCATTGAGCAGTACTCAGCAGGCCACCTGGACATGCT GGGCCGGATCAAGAGCCTGCAAACTCGGGTGGACCAAATTGTGGGTCGGGGGCCCGGGGACAGGAAGGCCCGGGAGAAGGGCGACAAGGGGCCCTCCGAC GCGGAGGTGGTGGATGAAATCAGCATGATGGGACGCGTGGTCAAGGTGGAGAAGCAGGTGCAGTCCATCGAGCACAAGCTGGACCTGCTGTTGGGCTTCT ATTCGCGCTGCCTGCGCTCTGGCACCTCGGCCAGCCTGGGCGCCGTGCAAGTGCCGCTGTTCGACCCCGACATCACCTCCGACTACCACAGCCCTGTGGA CCACGAGGACATCTCCGTCTCCGCACAGACGCTCAGCATCTCCCGCTCGGTCAGCACCAACATGGACTGAGGGACTTCTCAGAGGCAGGGCAGCACACGG CCAGCCCCGCGGCCTGGCGCTCCGACTGCCCTCTGAGGCCTCCGGACTCCTCTCGTACTTGAACTCACTCCCTCACGGGGAGAGAGACCACACGCAGTAT TGAGCTGCCTGAGTGGGCGTGGTACCTGCTGTGGG

refseq_KCNQ4.F1 (forward primer)
refseq_KCNQ4.R1 (reverse primer)
Single exon skipping

Frame 0

SHASLSAPSAPPPRTVPGPRRPQPGAAHGRGPP APPRPGSPARGRPPRGASGAHGRAERTGRGGRGR LPAPPRPPGQPPAAGRAPPWAGLRLGLRLRPAL LGRAQALPPPAELGLQRAGAAPRLGLRLPRLHI FAGLQLPGAVCAVHYPGAPGTCQRVSPHLGIRDD RGFRLGVHRPGLVRRMLLPLPRMAGSLPLCQKA LLCHRLHRVRGLGGRHRRGYPGQHLRHVRAAQH ALPADPAHGAHGPPRRHLEAAGLSGLRA*QGADH RLVHRVPGAHLRLLPGLPG*EGRQLRLLLLRRL ALVGDDYIDNHRLW*QDTAHMAGQGPGCWLRLT GHLFLCPACRHPRLRLCPEGPGAAPAEALREAED AGSQPHPGCLAPVLHRYEPGLPDSHLVLL*QYP PILQRAGPLV*ARATGPQWGPTAPGGAAGAGTR RSTLPLPARCHLPPAGQHLLLPWGKQPDGHQRPH PHGQLPAADGSFQAASGTSNNAHLPKQRAGG*G HQPHQGAKELELQ*PHPLPGISETQTPHLC*GC PLRGSSRGEELPV*AHGGRHHACCEDSHPLHQDS QVPGGQKEIQGDTATVRREGRH*AVLSRPPGHA GPDQEPANSGGPNCGSGARGQEGPGEGRQGALR RGGGG*NQHDGTRGQGGEAGAVHRAQAGPAVGLL FALPALWHLGQPGRRASAAVRPRHHLRLPQPCG PRGHLRLRTDAQHLPLGQHQHGLRDFSEAGQHT ASPAAWRSDCPLRPPDSSRT*THSLTGRETTRSI ELPEWAWYLLW

Frame 1

AMRL*APRARPRPGPCPGPGAPSPAPPMAEAPP RRLGLGPPPGDAPRAELVALTAVQSEQGEAGGG GSPRRLGLLGSPLPPGAPLPGPGSGSGSACGQRS SAAHKRYRRLQNWVYNVLERPRGWAFVYHVFIF LLVFSCLVLSVLSTIQEHQELANECLLILEFVM IVVFGLEYIVRVWSAGCCCRYRGWQGRFRFARKP FCVIDFIVFVASVAVIAAGTQGNIFATSALRSM RFLQILRMVRMDRRGGTWKLLGSVVYAHSKELI TAWYIGFLVLIFASFLVYLAEKDANSDFSSYADS LWWGTITLTTIGYGDKTPHTWLGRVLAAGFALL GISFFALPAGILGSGFALKVQEQHRQKHFEKRR MPAANLIQAAWRLYSTDMSRAYLTATWYYYDSIL PSFRELALLFEHVQRARNGGLRPLEVRRAPVPD GAPSRYPPVATCHRPGSTSFCPGESSRMGIKDR IRMGSSQRRTGPSKQHLAPPTMPTSPSSEQVGEA TSPTKVQKSWSFNDRTRFRASLRLKPRTSAEDA PSEEVAEEKSYQCELTVDDIMPAVKTVIRSIRI LKFLVAKRKFKETLRPYDVKDVIEQYSAGHLDML GRIKSLQTRVDQIVGRGPGDRKAREKGDKGPSD AEVVDEISMMGRVVKVEKQVQSIEHKLDLLLGF YSRCLRSGTSASLGAVQVPLFDPDITSDYHSPVD HEDISVSAQTLSISRSVSTNMD*GTSQRQGSTR PAPRPGAPTAL*GLRTPLVLELTPSRGERPHAV LSCLSGRGTCCG

Frame 2

PCVSERPERAPAPDRARAPAPPARRRPWPRPPR AASAWVPRPGTPPARS*WRSRPCRANRARRAGA APRAASASWAAPCRRARPSLGRAPARAPPAASA PRPRTSATAACRTGSTTCWSGPAAGPSSTTSSYF CWSSAAWCCLCCPLSRSTRNLPTSVSSSWNS** SWFSAWSTSSGSGPPDAAAATEDGRVASALPES PSVSSTSSCSWPRWPSSPRVPRATSSPRPRCAAC ASCRSCAWCAWTAAAAPGSCWAQWSTRIARS*S PPGTSGSWCSSSPPSWSTWLRRTPTPTSPPTPT RSGGGRLH*QPSAMVTRHRTHGWAGSWLLASPYW ASLSLPCLPAS*APALP*RSRSSTGRSTSRSGG CRQPTSSRLPGACTPPI*AGPT*QPPGTTMTVS SHPSESWPSCLSTCNGPAMGAYGPWRCGGRRYPT EHPPVTRPLPPATGRAAPPSALGKAAGWASKTA SAWAAPSGGRVLPSSIWHLQQCPPPQAASRWVR PPAPPRCKRAGASMTAPASGHL*DSNPAPLLRMP PQRK*QRRRATSVSSRWTTSCLL*RQSSAPSGF SSSWWPKGNSRRHCDRTT*RTSLSSTQQATWTC WAGSRACKLGWTKLWVGGPGTGRPGRRATRGPPT RRWWMKSA*WDAWSRWRSRCSPSSTSWTCCWAS IRAACALAPRPAWAPCKCRCSTPTSPPTTTALW TTRTSPSPHRRSASPARSAPTWTEGLLRGRAAHG QPRGLALRLPSEASGLLSYLNSLPHGERDHTQY *AA*VGVVPAV