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Sequences

Sequence NM_004518

CGCGGAGCGAGGTGGCCGCAGCGTCTCCGCGCGCGGCCCAAGCCCGGCAGGAGTGCGGAACCGCCGCCTCGGCCATGCGGCTCCCGGCCGGGGGGCCTGG GCTGGGGCCCGCGCCGCCCCCCGCGCTCCGCCCCCGCTGAGCCTGAGCCCGACCCGGGGCGCCTCCCGCCAGGCACCATGGTGCAGAAGTCGCGCAACGG CGGCGTATACCCCGGCCCGAGCGGGGAGAAGAAGCTGAAGGTGGGCTTCGTGGGGCTGGACCCCGGCGCGCCCGACTCCACCCGGGACGGGGCGCTGCTG ATCGCCGGCTCCGAGGCCCCCAAGCGCGGCAGCATCCTCAGCAAACCTCGCGCGGGCGGCGCGGGCGCCGGGAAGCCCCCCAAGCGCAACGCCTTCTACC GCAAGCTGCAGAATTTCCTCTACAACGTGCTGGAGCGGCCGCGCGGCTGGGCGTTCATCTACCACGCCTACGTGTTCCTCCTGGTTTTCTCCTGCCTCGT GCTGTCTGTGTTTTCCACCATCAAGGAGTATGAGAAGAGCTCGGAGGGGGCCCTCTACATCCTGGAAATCGTGACTATCGTGGTGTTTGGCGTGGAGTAC TTCGTGCGGATCTGGGCCGCAGGCTGCTGCTGCCGGTACCGTGGCTGGAGGGGGCGGCTCAAGTTTGCCCGGAAACCGTTCTGTGTGATTGACATCATGG TGCTCATCGCCTCCATTGCGGTGCTGGCCGCCGGCTCCCAGGGCAACGTCTTTGCCACATCTGCGCTCCGGAGCCTGCGCTTCCTGCAGATTCTGCGGAT GATCCGCATGGACCGGCGGGGAGGCACCTGGAAGCTGCTGGGCTCTGTGGTCTATGCCCACAGCAAGGAGCTGGTCACTGCCTGGTACATCGGCTTCCTT TGTCTCATCCTGGCCTCGTTCCTGGTGTACTTGGCAGAGAAGGGGGAGAACGACCACTTTGACACCTACGCGGATGCACTCTGGTGGGGCCTGATCACGC TGACCACCATTGGCTACGGGGACAAGTACCCCCAGACCTGGAACGGCAGGCTCCTTGCGGCAACCTTCACCCTCATCGGTGTCTCCTTCTTCGCGCTGCC TGCAGGCATCTTGGGGTCTGGGTTTGCCCTGAAGGTTCAGGAGCAGCACAGGCAGAAGCACTTTGAGAAGAGGCGGAACCCGGCAGCAGGCCTGATCCAG TCGGCCTGGAGATTCTACGCCACCAACCTCTCGCGCACAGACCTGCACTCCACGTGGCAGTACTACGAGCGAACGGTCACCGTGCCCATGTACAGACTTA TCCCCCCGCTGAACCAGCTGGAGCTGCTGAGGAACCTCAAGAGTAAATCTGGACTCGCTTTCAGGAAGGACCCCCCGCCGGAGCCGTCTCCAAGCCAGAA GGTCAGTTTGAAAGATCGTGTCTTCTCCAGCCCCCGAGGCGTGGCTGCCAAGGGGAAGGGGTCCCCGCAGGCCCAGACTGTGAGGCGGTCACCCAGCGCC GACCAGAGCCTCGAGGACAGCCCCAGCAAGGTGCCCAAGAGCTGGAGCTTCGGGGACCGCAGCCGGGCACGCCAGGCTTTCCGCATCAAGGGTGCCGCGT CACGGCAGAACTCAGAAGAAGCAAGCCTCCCCGGAGAGGACATTGTGGATGACAAGAGCTGCCCCTGCGAGTTTGTGACCGAGGACCTGACCCCGGGCCT CAAAGTCAGCATCAGAGCCGTGTGTGTCATGCGGTTCCTGGTGTCCAAGCGGAAGTTCAAGGAGAGCCTGCGGCCCTACGACGTGATGGACGTCATCGAG CAGTACTCAGCCGGCCACCTGGACATGCTGTCCCGAATTAAGAGCCTGCAGTCCAGAGTGGACCAGATCGTGGGGCGGGGCCCAGCGATCACGGACAAGG ACCGCACCAAGGGCCCGGCCGAGGCGGAGCTGCCCGAGGACCCCAGCATGATGGGACGGCTCGGGAAGGTGGAGAAGCAGGTCTTGTCCATGGAGAAGAA GCTGGACTTCCTGGTGAATATCTACATGCAGCGGATGGGCATCCCCCCGACAGAGACCGAGGCCTACTTTGGGGCCAAAGAGCCGGAGCCGGCGCCGCCG TACCACAGCCCGGAAGACAGCCGGGAGCATGTCGACAGGCACGGCTGCATTGTCAAGATCGTGCGCTCCAGCAGCTCCACGGGCCAGAAGAACTTCTCGG CGCCCCCGGCCGCGCCCCCTGTCCAGTGTCCGCCCTCCACCTCCTGGCAGCCACAGAGCCACCCGCGCCAGGGCCACGGCACCTCCCCCGTGGGGGACCA CGGCTCCCTGGTGCGCATCCCGCCGCCGCCTGCCCACGAGCGGTCGCTGTCCGCCTACGGCGGGGGCAACCGCGCCAGCATGGAGTTCCTGCGGCAGGAG GACACCCCGGGCTGCAGGCCCCCCGAGGGGAACCTGCGGGACAGCGACACGTCCATCTCCATCCCGTCCGTGGACCACGAGGAGCTGGAGCGTTCCTTCA GCGGCTTCAGCATCTCCCAGTCCAAGGAGAACCTGGATGCTCTCAACAGCTGCTACGCGGCCGTGGCGCCTTGTGCCAAAGTCAGGCCCTACATTGCGGA GGGAGAGTCAGACACCGACTCCGACCTCTGTACCCCGTGCGGGCCCCCGCCACGCTCGGCCACCGGCGAGGGTCCCTTTGGTGACGTGGGCTGGGCCGGG CCCAGGAAGTGAGGCGGCGCTGGGCCAGTGGACCCGCCCGCGGCCCTCCTCAGCACGGTGCCTCCGAGGTTTTGAGGCGGGAACCCTCTGGGGCCCTTTT CTTACAGTAACTGAGTGTGGCGGGAAGGGTGGGCCCTGGAGGGGCCCATGTGGGCTGAAGGATGGGGGCTCCTGGCAGTGACCTTTTACAAAAGTTATTT TCCAACAGGGGCTGGAGGGCTGGGCAGGGCCCTGTGGCTCCAGGAGCAGCGTGCAGGAGCAAGGCTGCCCTGTCCACTCTGCTCAGGGCCGCGGCCGACA TCAGCCCGGTGTGAGGAGGGGCGGGAGTGATGACGGGGTGTTGCCAGCGTGGCAACAGGCGGGGGGTTGTCTCAGCCGAGCCCAGGGGAGGCACAAAGGG CAGGCCTGTTCCCTGAGGACCTGCGCAAAGGGCGGGCCTGTTTGGTGAGGACCTGCGGCCTTGGGTC

refseq_KCNQ2.F1 (forward primer)
refseq_KCNQ2.R1 (reverse primer)
Single exon skipping

Frame 0

RGARWPQRLRARPKPGRSAEPPPRPCGSRPGGL GWGPRRPPRSAPAEPEPDPGRLPPGTMVQKSRNG GVYPGPSGEKKLKVGFVGLDPGAPDSTRDGALL IAGSEAPKRGSILSKPRAGGAGAGKPPKRNAFY RKLQNFLYNVLERPRGWAFIYHAYVFLLVFSCLV LSVFSTIKEYEKSSEGALYILEIVTIVVFGVEY FVRIWAAGCCCRYRGWRGRLKFARKPFCVIDIM VLIASIAVLAAGSQGNVFATSALRSLRFLQILRM IRMDRRGGTWKLLGSVVYAHSKELVTAWYIGFL CLILASFLVYLAEKGENDHFDTYADALWWGLIT LTTIGYGDKYPQTWNGRLLAATFTLIGVSFFALP AGILGSGFALKVQEQHRQKHFEKRRNPAAGLIQ SAWRFYATNLSRTDLHSTWQYYERTVTVPMYRL IPPLNQLELLRNLKSKSGLAFRKDPPPEPSPSQK VSLKDRVFSSPRGVAAKGKGSPQAQTVRRSPSA DQSLEDSPSKVPKSWSFGDRSRARQAFRIKGAA SRQNSEEASLPGEDIVDDKSCPCEFVTEDLTPGL KVSIRAVCVMRFLVSKRKFKESLRPYDVMDVIE QYSAGHLDMLSRIKSLQSRVDQIVGRGPAITDK DRTKGPAEAELPEDPSMMGRLGKVEKQVLSMEKK LDFLVNIYMQRMGIPPTETEAYFGAKEPEPAPP YHSPEDSREHVDRHGCIVKIVRSSSSTGQKNFS APPAAPPVQCPPSTSWQPQSHPRQGHGTSPVGDH GSLVRIPPPPAHERSLSAYGGGNRASMEFLRQE DTPGCRPPEGNLRDSDTSISIPSVDHEELERSF SGFSISQSKENLDALNSCYAAVAPCAKVRPYIAE GESDTDSDLCTPCGPPPRSATGEGPFGDVGWAG PRK*GGAGPVDPPAALLSTVPPRF*GGNPLGPF SYSN*VWREGWALEGPMWAEGWGLLAVTFYKSYF PTGAGGLGRALWLQEQRAGARLPCPLCSGPRPT SARCEEGRE**RGVASVATGGGLSQPSPGEAQR AGLFPEDLRKGRACLVRTCGLG

Frame 1

AERGGRSVSARGPSPAGVRNRRLGHAAPGRGAW AGARAAPRAPPPLSLSPTRGASRQAPWCRSRAT AAYTPARAGRRS*RWASWGWTPARPTPPGTGRC* SPAPRPPSAAASSANLARAARAPGSPPSATPST ASCRISSTTCWSGRAAGRSSTTPTCSSWFSPAS CCLCFPPSRSMRRARRGPSTSWKS*LSWCLAWST SCGSGPQAAAAGTVAGGGGSSLPGNRSV*LTSW CSSPPLRCWPPAPRATSLPHLRSGACASCRFCG *SAWTGGEAPGSCWALWSMPTARSWSLPGTSASF VSSWPRSWCTWQRRGRTTTLTPTRMHSGGA*SR *PPLATGTSTPRPGTAGSLRQPSPSSVSPSSRC LQASWGLGLP*RFRSSTGRSTLRRGGTRQQA*SS RPGDSTPPTSRAQTCTPRGSTTSERSPCPCTDL SPR*TSWSC*GTSRVNLDSLSGRTPRRSRLQAR RSV*KIVSSPAPEAWLPRGRGPRRPRL*GGHPAP TRASRTAPARCPRAGASGTAAGHARLSASRVPR HGRTQKKQASPERTLWMTRAAPASL*PRT*PRA SKSASEPCVSCGSWCPSGSSRRACGPTT*WTSSS STQPATWTCCPELRACSPEWTRSWGGAQRSRTR TAPRARPRRSCPRTPA*WDGSGRWRSRSCPWRR SWTSW*ISTCSGWASPRQRPRPTLGPKSRSRRRR TTARKTAGSMSTGTAALSRSCAPAAPRARRTSR RPRPRPLSSVRPPPPGSHRATRARATAPPPWGT TAPWCASRRRLPTSGRCPPTAGATAPAWSSCGRR TPRAAGPPRGTCGTATRPSPSRPWTTRSWSVPS AASASPSPRRTWMLSTAATRPWRLVPKSGPTLR RESQTPTPTSVPRAGPRHARPPARVPLVTWAGPG PGSEAALGQWTRPRPSSARCLRGFEAGTLWGPF LTVTECGGKGGPWRGPCGLKDGGSWQ*PFTKVI FQQGLEGWAGPCGSRSSVQEQGCPVHSAQGRGRH QPGVRRGGSDDGVLPAWQQAGGCLSRAQGRHKG QACSLRTCAKGGPVW*GPAALG

Frame 2

RSEVAAASPRAAQARQECGTAASAMRLPAGGPG LGPAPPPALRPR*A*ARPGAPPARHHGAEVAQR RRIPRPERGEEAEGGLRGAGPRRARLHPGRGAA DRRLRGPQARQHPQQTSRGRRGRREAPQAQRLLP QAAEFPLQRAGAAARLGVHLPRLRVPPGFLLPR AVCVFHHQGV*EELGGGPLHPGNRDYRGVWRGV LRADLGRRLLLPVPWLEGAAQVCPETVLCD*HHG AHRLHCGAGRRLPGQRLCHICAPEPALPADSAD DPHGPAGRHLEAAGLCGLCPQQGAGHCLVHRLP LSHPGLVPGVLGREGGERPL*HLRGCTLVGPDHA DHHWLRGQVPPDLERQAPCGNLHPHRCLLLRAA CRHLGVWVCPEGSGAAQAEAL*EEAEPGSRPDP VGLEILRHQPLAHRPALHVAVLRANGHRAHVQTY PPAEPAGAAEEPQE*IWTRFQEGPPAGAVSKPE GQFERSCLLQPPRRGCQGEGVPAGPDCEAVTQR RPEPRGQPQQGAQELELRGPQPGTPGFPHQGCRV TAELRRSKPPRRGHCG*QELPLRVCDRGPDPGP QSQHQSRVCHAVPGVQAEVQGEPAALRRDGRHR AVLSRPPGHAVPN*EPAVQSGPDRGAGPSDHGQG PHQGPGRGGAARGPQHDGTAREGGEAGLVHGEE AGLPGEYLHAADGHPPDRDRGLLWGQRAGAGAA VPQPGRQPGACRQARLHCQDRALQQLHGPEELLG APGRAPCPVSALHLLAATEPPAPGPRHLPRGGP RLPGAHPAAACPRAVAVRLRRGQPRQHGVPAAG GHPGLQAPRGEPAGQRHVHLHPVRGPRGAGAFLQ RLQHLPVQGEPGCSQQLLRGRGALCQSQALHCG GRVRHRLRPLYPVRAPATLGHRRGSLW*RGLGR AQEVRRRWASGPARGPPQHGASEVLRREPSGALF LQ*LSVAGRVGPGGAHVG*RMGAPGSDLLQKLF SNRGWRAGQGPVAPGAACRSKAALSTLLRAAAD ISPV*GGAGVMTGCCQRGNRRGVVSAEPRGGTKG RPVP*GPAQRAGLFGEDLRPWV

Sequence NM_172108

CGCGGAGCGAGGTGGCCGCAGCGTCTCCGCGCGCGGCCCAAGCCCGGCAGGAGTGCGGAACCGCCGCCTCGGCCATGCGGCTCCCGGCCGGGGGGCCTGG GCTGGGGCCCGCGCCGCCCCCCGCGCTCCGCCCCCGCTGAGCCTGAGCCCGACCCGGGGCGCCTCCCGCCAGGCACCATGGTGCAGAAGTCGCGCAACGG CGGCGTATACCCCGGCCCGAGCGGGGAGAAGAAGCTGAAGGTGGGCTTCGTGGGGCTGGACCCCGGCGCGCCCGACTCCACCCGGGACGGGGCGCTGCTG ATCGCCGGCTCCGAGGCCCCCAAGCGCGGCAGCATCCTCAGCAAACCTCGCGCGGGCGGCGCGGGCGCCGGGAAGCCCCCCAAGCGCAACGCCTTCTACC GCAAGCTGCAGAATTTCCTCTACAACGTGCTGGAGCGGCCGCGCGGCTGGGCGTTCATCTACCACGCCTACGTGTTCCTCCTGGTTTTCTCCTGCCTCGT GCTGTCTGTGTTTTCCACCATCAAGGAGTATGAGAAGAGCTCGGAGGGGGCCCTCTACATCCTGGAAATCGTGACTATCGTGGTGTTTGGCGTGGAGTAC TTCGTGCGGATCTGGGCCGCAGGCTGCTGCTGCCGGTACCGTGGCTGGAGGGGGCGGCTCAAGTTTGCCCGGAAACCGTTCTGTGTGATTGACATCATGG TGCTCATCGCCTCCATTGCGGTGCTGGCCGCCGGCTCCCAGGGCAACGTCTTTGCCACATCTGCGCTCCGGAGCCTGCGCTTCCTGCAGATTCTGCGGAT GATCCGCATGGACCGGCGGGGAGGCACCTGGAAGCTGCTGGGCTCTGTGGTCTATGCCCACAGCAAGGAGCTGGTCACTGCCTGGTACATCGGCTTCCTT TGTCTCATCCTGGCCTCGTTCCTGGTGTACTTGGCAGAGAAGGGGGAGAACGACCACTTTGACACCTACGCGGATGCACTCTGGTGGGGCCTGATCACGC TGACCACCATTGGCTACGGGGACAAGTACCCCCAGACCTGGAACGGCAGGCTCCTTGCGGCAACCTTCACCCTCATCGGTGTCTCCTTCTTCGCGCTGCC TGCAGGCATCTTGGGGTCTGGGTTTGCCCTGAAGGTTCAGGAGCAGCACAGGCAGAAGCACTTTGAGAAGAGGCGGAACCCGGCAGCAGGCCTGATCCAG TCGGCCTGGAGATTCTACGCCACCAACCTCTCGCGCACAGACCTGCACTCCACGTGGCAGTACTACGAGCGAACGGTCACCGTGCCCATGTACAGTTCGC AAACTCAAACCTACGGGGCCTCCAGACTTATCCCCCCGCTGAACCAGCTGGAGCTGCTGAGGAACCTCAAGAGTAAATCTGGACTCGCTTTCAGGAAGGA CCCCCCGCCGGAGCCGTCTCCAAGCCCCCGAGGCGTGGCTGCCAAGGGGAAGGGGTCCCCGCAGGCCCAGACTGTGAGGCGGTCACCCAGCGCCGACCAG AGCCTCGAGGACAGCCCCAGCAAGGTGCCCAAGAGCTGGAGCTTCGGGGACCGCAGCCGGGCACGCCAGGCTTTCCGCATCAAGGGTGCCGCGTCACGGC AGAACTCAGAAGCAAGCCTCCCCGGAGAGGACATTGTGGATGACAAGAGCTGCCCCTGCGAGTTTGTGACCGAGGACCTGACCCCGGGCCTCAAAGTCAG CATCAGAGCCGTGTGTGTCATGCGGTTCCTGGTGTCCAAGCGGAAGTTCAAGGAGAGCCTGCGGCCCTACGACGTGATGGACGTCATCGAGCAGTACTCA GCCGGCCACCTGGACATGCTGTCCCGAATTAAGAGCCTGCAGTCCAGAGTGGACCAGATCGTGGGGCGGGGCCCAGCGATCACGGACAAGGACCGCACCA AGGGCCCGGCCGAGGCGGAGCTGCCCGAGGACCCCAGCATGATGGGACGGCTCGGGAAGGTGGAGAAGCAGGTCTTGTCCATGGAGAAGAAGCTGGACTT CCTGGTGAATATCTACATGCAGCGGATGGGCATCCCCCCGACAGAGACCGAGGCCTACTTTGGGGCCAAAGAGCCGGAGCCGGCGCCGCCGTACCACAGC CCGGAAGACAGCCGGGAGCATGTCGACAGGCACGGCTGCATTGTCAAGATCGTGCGCTCCAGCAGCTCCACGGGCCAGAAGAACTTCTCGGCGCCCCCGG CCGCGCCCCCTGTCCAGTGTCCGCCCTCCACCTCCTGGCAGCCACAGAGCCACCCGCGCCAGGGCCACGGCACCTCCCCCGTGGGGGACCACGGCTCCCT GGTGCGCATCCCGCCGCCGCCTGCCCACGAGCGGTCGCTGTCCGCCTACGGCGGGGGCAACCGCGCCAGCATGGAGTTCCTGCGGCAGGAGGACACCCCG GGCTGCAGGCCCCCCGAGGGGAACCTGCGGGACAGCGACACGTCCATCTCCATCCCGTCCGTGGACCACGAGGAGCTGGAGCGTTCCTTCAGCGGCTTCA GCATCTCCCAGTCCAAGGAGAACCTGGATGCTCTCAACAGCTGCTACGCGGCCGTGGCGCCTTGTGCCAAAGTCAGGCCCTACATTGCGGAGGGAGAGTC AGACACCGACTCCGACCTCTGTACCCCGTGCGGGCCCCCGCCACGCTCGGCCACCGGCGAGGGTCCCTTTGGTGACGTGGGCTGGGCCGGGCCCAGGAAG TGAGGCGGCGCTGGGCCAGTGGACCCGCCCGCGGCCCTCCTCAGCACGGTGCCTCCGAGGTTTTGAGGCGGGAACCCTCTGGGGCCCTTTTCTTACAGTA ACTGAGTGTGGCGGGAAGGGTGGGCCCTGGAGGGGCCCATGTGGGCTGAAGGATGGGGGCTCCTGGCAGTGACCTTTTACAAAAGTTATTTTCCAACAGG GGCTGGAGGGCTGGGCAGGGCCCTGTGGCTCCAGGAGCAGCGTGCAGGAGCAAGGCTGCCCTGTCCACTCTGCTCAGGGCCGCGGCCGACATCAGCCCGG TGTGAGGAGGGGCGGGAGTGATGACGGGGTGTTGCCAGCGTGGCAACAGGCGGGGGGTTGTCTCAGCCGAGCCCAGGGGAGGCACAAAGGGCAGGCCTGT TCCCTGAGGACCTGCGCAAAGGGCGGGCCTGTTTGGTGAGGACCTGCGGCCTTGGGTC

refseq_KCNQ2.F1 (forward primer)
refseq_KCNQ2.R1 (reverse primer)
Single exon skipping

Frame 0

RGARWPQRLRARPKPGRSAEPPPRPCGSRPGGL GWGPRRPPRSAPAEPEPDPGRLPPGTMVQKSRNG GVYPGPSGEKKLKVGFVGLDPGAPDSTRDGALL IAGSEAPKRGSILSKPRAGGAGAGKPPKRNAFY RKLQNFLYNVLERPRGWAFIYHAYVFLLVFSCLV LSVFSTIKEYEKSSEGALYILEIVTIVVFGVEY FVRIWAAGCCCRYRGWRGRLKFARKPFCVIDIM VLIASIAVLAAGSQGNVFATSALRSLRFLQILRM IRMDRRGGTWKLLGSVVYAHSKELVTAWYIGFL CLILASFLVYLAEKGENDHFDTYADALWWGLIT LTTIGYGDKYPQTWNGRLLAATFTLIGVSFFALP AGILGSGFALKVQEQHRQKHFEKRRNPAAGLIQ SAWRFYATNLSRTDLHSTWQYYERTVTVPMYSS QTQTYGASRLIPPLNQLELLRNLKSKSGLAFRKD PPPEPSPSPRGVAAKGKGSPQAQTVRRSPSADQ SLEDSPSKVPKSWSFGDRSRARQAFRIKGAASR QNSEASLPGEDIVDDKSCPCEFVTEDLTPGLKVS IRAVCVMRFLVSKRKFKESLRPYDVMDVIEQYS AGHLDMLSRIKSLQSRVDQIVGRGPAITDKDRT KGPAEAELPEDPSMMGRLGKVEKQVLSMEKKLDF LVNIYMQRMGIPPTETEAYFGAKEPEPAPPYHS PEDSREHVDRHGCIVKIVRSSSSTGQKNFSAPP AAPPVQCPPSTSWQPQSHPRQGHGTSPVGDHGSL VRIPPPPAHERSLSAYGGGNRASMEFLRQEDTP GCRPPEGNLRDSDTSISIPSVDHEELERSFSGF SISQSKENLDALNSCYAAVAPCAKVRPYIAEGES DTDSDLCTPCGPPPRSATGEGPFGDVGWAGPRK *GGAGPVDPPAALLSTVPPRF*GGNPLGPFSYS N*VWREGWALEGPMWAEGWGLLAVTFYKSYFPTG AGGLGRALWLQEQRAGARLPCPLCSGPRPTSAR CEEGRE**RGVASVATGGGLSQPSPGEAQRAGL FPEDLRKGRACLVRTCGLG

Frame 1

AERGGRSVSARGPSPAGVRNRRLGHAAPGRGAW AGARAAPRAPPPLSLSPTRGASRQAPWCRSRAT AAYTPARAGRRS*RWASWGWTPARPTPPGTGRC* SPAPRPPSAAASSANLARAARAPGSPPSATPST ASCRISSTTCWSGRAAGRSSTTPTCSSWFSPAS CCLCFPPSRSMRRARRGPSTSWKS*LSWCLAWST SCGSGPQAAAAGTVAGGGGSSLPGNRSV*LTSW CSSPPLRCWPPAPRATSLPHLRSGACASCRFCG *SAWTGGEAPGSCWALWSMPTARSWSLPGTSASF VSSWPRSWCTWQRRGRTTTLTPTRMHSGGA*SR *PPLATGTSTPRPGTAGSLRQPSPSSVSPSSRC LQASWGLGLP*RFRSSTGRSTLRRGGTRQQA*SS RPGDSTPPTSRAQTCTPRGSTTSERSPCPCTVR KLKPTGPPDLSPR*TSWSC*GTSRVNLDSLSGR TPRRSRLQAPEAWLPRGRGPRRPRL*GGHPAPTR ASRTAPARCPRAGASGTAAGHARLSASRVPRHG RTQKQASPERTLWMTRAAPASL*PRT*PRASKS ASEPCVSCGSWCPSGSSRRACGPTT*WTSSSSTQ PATWTCCPELRACSPEWTRSWGGAQRSRTRTAP RARPRRSCPRTPA*WDGSGRWRSRSCPWRRSWT SW*ISTCSGWASPRQRPRPTLGPKSRSRRRRTTA RKTAGSMSTGTAALSRSCAPAAPRARRTSRRPR PRPLSSVRPPPPGSHRATRARATAPPPWGTTAP WCASRRRLPTSGRCPPTAGATAPAWSSCGRRTPR AAGPPRGTCGTATRPSPSRPWTTRSWSVPSAAS ASPSPRRTWMLSTAATRPWRLVPKSGPTLRRES QTPTPTSVPRAGPRHARPPARVPLVTWAGPGPGS EAALGQWTRPRPSSARCLRGFEAGTLWGPFLTV TECGGKGGPWRGPCGLKDGGSWQ*PFTKVIFQQ GLEGWAGPCGSRSSVQEQGCPVHSAQGRGRHQPG VRRGGSDDGVLPAWQQAGGCLSRAQGRHKGQAC SLRTCAKGGPVW*GPAALG

Frame 2

RSEVAAASPRAAQARQECGTAASAMRLPAGGPG LGPAPPPALRPR*A*ARPGAPPARHHGAEVAQR RRIPRPERGEEAEGGLRGAGPRRARLHPGRGAA DRRLRGPQARQHPQQTSRGRRGRREAPQAQRLLP QAAEFPLQRAGAAARLGVHLPRLRVPPGFLLPR AVCVFHHQGV*EELGGGPLHPGNRDYRGVWRGV LRADLGRRLLLPVPWLEGAAQVCPETVLCD*HHG AHRLHCGAGRRLPGQRLCHICAPEPALPADSAD DPHGPAGRHLEAAGLCGLCPQQGAGHCLVHRLP LSHPGLVPGVLGREGGERPL*HLRGCTLVGPDHA DHHWLRGQVPPDLERQAPCGNLHPHRCLLLRAA CRHLGVWVCPEGSGAAQAEAL*EEAEPGSRPDP VGLEILRHQPLAHRPALHVAVLRANGHRAHVQFA NSNLRGLQTYPPAEPAGAAEEPQE*IWTRFQEG PPAGAVSKPPRRGCQGEGVPAGPDCEAVTQRRP EPRGQPQQGAQELELRGPQPGTPGFPHQGCRVTA ELRSKPPRRGHCG*QELPLRVCDRGPDPGPQSQ HQSRVCHAVPGVQAEVQGEPAALRRDGRHRAVL SRPPGHAVPN*EPAVQSGPDRGAGPSDHGQGPHQ GPGRGGAARGPQHDGTAREGGEAGLVHGEEAGL PGEYLHAADGHPPDRDRGLLWGQRAGAGAAVPQ PGRQPGACRQARLHCQDRALQQLHGPEELLGAPG RAPCPVSALHLLAATEPPAPGPRHLPRGGPRLP GAHPAAACPRAVAVRLRRGQPRQHGVPAAGGHP GLQAPRGEPAGQRHVHLHPVRGPRGAGAFLQRLQ HLPVQGEPGCSQQLLRGRGALCQSQALHCGGRV RHRLRPLYPVRAPATLGHRRGSLW*RGLGRAQE VRRRWASGPARGPPQHGASEVLRREPSGALFLQ* LSVAGRVGPGGAHVG*RMGAPGSDLLQKLFSNR GWRAGQGPVAPGAACRSKAALSTLLRAAADISP V*GGAGVMTGCCQRGNRRGVVSAEPRGGTKGRPV P*GPAQRAGLFGEDLRPWV

Sequence NM_172107

CGCGGAGCGAGGTGGCCGCAGCGTCTCCGCGCGCGGCCCAAGCCCGGCAGGAGTGCGGAACCGCCGCCTCGGCCATGCGGCTCCCGGCCGGGGGGCCTGG GCTGGGGCCCGCGCCGCCCCCCGCGCTCCGCCCCCGCTGAGCCTGAGCCCGACCCGGGGCGCCTCCCGCCAGGCACCATGGTGCAGAAGTCGCGCAACGG CGGCGTATACCCCGGCCCGAGCGGGGAGAAGAAGCTGAAGGTGGGCTTCGTGGGGCTGGACCCCGGCGCGCCCGACTCCACCCGGGACGGGGCGCTGCTG ATCGCCGGCTCCGAGGCCCCCAAGCGCGGCAGCATCCTCAGCAAACCTCGCGCGGGCGGCGCGGGCGCCGGGAAGCCCCCCAAGCGCAACGCCTTCTACC GCAAGCTGCAGAATTTCCTCTACAACGTGCTGGAGCGGCCGCGCGGCTGGGCGTTCATCTACCACGCCTACGTGTTCCTCCTGGTTTTCTCCTGCCTCGT GCTGTCTGTGTTTTCCACCATCAAGGAGTATGAGAAGAGCTCGGAGGGGGCCCTCTACATCCTGGAAATCGTGACTATCGTGGTGTTTGGCGTGGAGTAC TTCGTGCGGATCTGGGCCGCAGGCTGCTGCTGCCGGTACCGTGGCTGGAGGGGGCGGCTCAAGTTTGCCCGGAAACCGTTCTGTGTGATTGACATCATGG TGCTCATCGCCTCCATTGCGGTGCTGGCCGCCGGCTCCCAGGGCAACGTCTTTGCCACATCTGCGCTCCGGAGCCTGCGCTTCCTGCAGATTCTGCGGAT GATCCGCATGGACCGGCGGGGAGGCACCTGGAAGCTGCTGGGCTCTGTGGTCTATGCCCACAGCAAGGAGCTGGTCACTGCCTGGTACATCGGCTTCCTT TGTCTCATCCTGGCCTCGTTCCTGGTGTACTTGGCAGAGAAGGGGGAGAACGACCACTTTGACACCTACGCGGATGCACTCTGGTGGGGCCTGATCACGC TGACCACCATTGGCTACGGGGACAAGTACCCCCAGACCTGGAACGGCAGGCTCCTTGCGGCAACCTTCACCCTCATCGGTGTCTCCTTCTTCGCGCTGCC TGCAGGCATCTTGGGGTCTGGGTTTGCCCTGAAGGTTCAGGAGCAGCACAGGCAGAAGCACTTTGAGAAGAGGCGGAACCCGGCAGCAGGCCTGATCCAG TCGGCCTGGAGATTCTACGCCACCAACCTCTCGCGCACAGACCTGCACTCCACGTGGCAGTACTACGAGCGAACGGTCACCGTGCCCATGTACAGTTCGC AAACTCAAACCTACGGGGCCTCCAGACTTATCCCCCCGCTGAACCAGCTGGAGCTGCTGAGGAACCTCAAGAGTAAATCTGGACTCGCTTTCAGGAAGGA CCCCCCGCCGGAGCCGTCTCCAAGTAAAGGCAGCCCGTGCAGAGGGCCCCTGTGTGGATGCTGCCCCGGACGCTCTAGCCAGAAGGTCAGTTTGAAAGAT CGTGTCTTCTCCAGCCCCCGAGGCGTGGCTGCCAAGGGGAAGGGGTCCCCGCAGGCCCAGACTGTGAGGCGGTCACCCAGCGCCGACCAGAGCCTCGAGG ACAGCCCCAGCAAGGTGCCCAAGAGCTGGAGCTTCGGGGACCGCAGCCGGGCACGCCAGGCTTTCCGCATCAAGGGTGCCGCGTCACGGCAGAACTCAGA AGAAGCAAGCCTCCCCGGAGAGGACATTGTGGATGACAAGAGCTGCCCCTGCGAGTTTGTGACCGAGGACCTGACCCCGGGCCTCAAAGTCAGCATCAGA GCCGTGTGTGTCATGCGGTTCCTGGTGTCCAAGCGGAAGTTCAAGGAGAGCCTGCGGCCCTACGACGTGATGGACGTCATCGAGCAGTACTCAGCCGGCC ACCTGGACATGCTGTCCCGAATTAAGAGCCTGCAGTCCAGAGTGGACCAGATCGTGGGGCGGGGCCCAGCGATCACGGACAAGGACCGCACCAAGGGCCC GGCCGAGGCGGAGCTGCCCGAGGACCCCAGCATGATGGGACGGCTCGGGAAGGTGGAGAAGCAGGTCTTGTCCATGGAGAAGAAGCTGGACTTCCTGGTG AATATCTACATGCAGCGGATGGGCATCCCCCCGACAGAGACCGAGGCCTACTTTGGGGCCAAAGAGCCGGAGCCGGCGCCGCCGTACCACAGCCCGGAAG ACAGCCGGGAGCATGTCGACAGGCACGGCTGCATTGTCAAGATCGTGCGCTCCAGCAGCTCCACGGGCCAGAAGAACTTCTCGGCGCCCCCGGCCGCGCC CCCTGTCCAGTGTCCGCCCTCCACCTCCTGGCAGCCACAGAGCCACCCGCGCCAGGGCCACGGCACCTCCCCCGTGGGGGACCACGGCTCCCTGGTGCGC ATCCCGCCGCCGCCTGCCCACGAGCGGTCGCTGTCCGCCTACGGCGGGGGCAACCGCGCCAGCATGGAGTTCCTGCGGCAGGAGGACACCCCGGGCTGCA GGCCCCCCGAGGGGAACCTGCGGGACAGCGACACGTCCATCTCCATCCCGTCCGTGGACCACGAGGAGCTGGAGCGTTCCTTCAGCGGCTTCAGCATCTC CCAGTCCAAGGAGAACCTGGATGCTCTCAACAGCTGCTACGCGGCCGTGGCGCCTTGTGCCAAAGTCAGGCCCTACATTGCGGAGGGAGAGTCAGACACC GACTCCGACCTCTGTACCCCGTGCGGGCCCCCGCCACGCTCGGCCACCGGCGAGGGTCCCTTTGGTGACGTGGGCTGGGCCGGGCCCAGGAAGTGAGGCG GCGCTGGGCCAGTGGACCCGCCCGCGGCCCTCCTCAGCACGGTGCCTCCGAGGTTTTGAGGCGGGAACCCTCTGGGGCCCTTTTCTTACAGTAACTGAGT GTGGCGGGAAGGGTGGGCCCTGGAGGGGCCCATGTGGGCTGAAGGATGGGGGCTCCTGGCAGTGACCTTTTACAAAAGTTATTTTCCAACAGGGGCTGGA GGGCTGGGCAGGGCCCTGTGGCTCCAGGAGCAGCGTGCAGGAGCAAGGCTGCCCTGTCCACTCTGCTCAGGGCCGCGGCCGACATCAGCCCGGTGTGAGG AGGGGCGGGAGTGATGACGGGGTGTTGCCAGCGTGGCAACAGGCGGGGGGTTGTCTCAGCCGAGCCCAGGGGAGGCACAAAGGGCAGGCCTGTTCCCTGA GGACCTGCGCAAAGGGCGGGCCTGTTTGGTGAGGACCTGCGGCCTTGGGTC

refseq_KCNQ2.F1 (forward primer)
refseq_KCNQ2.R1 (reverse primer)
Single exon skipping

Frame 0

RGARWPQRLRARPKPGRSAEPPPRPCGSRPGGL GWGPRRPPRSAPAEPEPDPGRLPPGTMVQKSRNG GVYPGPSGEKKLKVGFVGLDPGAPDSTRDGALL IAGSEAPKRGSILSKPRAGGAGAGKPPKRNAFY RKLQNFLYNVLERPRGWAFIYHAYVFLLVFSCLV LSVFSTIKEYEKSSEGALYILEIVTIVVFGVEY FVRIWAAGCCCRYRGWRGRLKFARKPFCVIDIM VLIASIAVLAAGSQGNVFATSALRSLRFLQILRM IRMDRRGGTWKLLGSVVYAHSKELVTAWYIGFL CLILASFLVYLAEKGENDHFDTYADALWWGLIT LTTIGYGDKYPQTWNGRLLAATFTLIGVSFFALP AGILGSGFALKVQEQHRQKHFEKRRNPAAGLIQ SAWRFYATNLSRTDLHSTWQYYERTVTVPMYSS QTQTYGASRLIPPLNQLELLRNLKSKSGLAFRKD PPPEPSPSKGSPCRGPLCGCCPGRSSQKVSLKD RVFSSPRGVAAKGKGSPQAQTVRRSPSADQSLE DSPSKVPKSWSFGDRSRARQAFRIKGAASRQNSE EASLPGEDIVDDKSCPCEFVTEDLTPGLKVSIR AVCVMRFLVSKRKFKESLRPYDVMDVIEQYSAG HLDMLSRIKSLQSRVDQIVGRGPAITDKDRTKGP AEAELPEDPSMMGRLGKVEKQVLSMEKKLDFLV NIYMQRMGIPPTETEAYFGAKEPEPAPPYHSPE DSREHVDRHGCIVKIVRSSSSTGQKNFSAPPAAP PVQCPPSTSWQPQSHPRQGHGTSPVGDHGSLVR IPPPPAHERSLSAYGGGNRASMEFLRQEDTPGC RPPEGNLRDSDTSISIPSVDHEELERSFSGFSIS QSKENLDALNSCYAAVAPCAKVRPYIAEGESDT DSDLCTPCGPPPRSATGEGPFGDVGWAGPRK*G GAGPVDPPAALLSTVPPRF*GGNPLGPFSYSN*V WREGWALEGPMWAEGWGLLAVTFYKSYFPTGAG GLGRALWLQEQRAGARLPCPLCSGPRPTSARCE EGRE**RGVASVATGGGLSQPSPGEAQRAGLFPE DLRKGRACLVRTCGLG

Frame 1

AERGGRSVSARGPSPAGVRNRRLGHAAPGRGAW AGARAAPRAPPPLSLSPTRGASRQAPWCRSRAT AAYTPARAGRRS*RWASWGWTPARPTPPGTGRC* SPAPRPPSAAASSANLARAARAPGSPPSATPST ASCRISSTTCWSGRAAGRSSTTPTCSSWFSPAS CCLCFPPSRSMRRARRGPSTSWKS*LSWCLAWST SCGSGPQAAAAGTVAGGGGSSLPGNRSV*LTSW CSSPPLRCWPPAPRATSLPHLRSGACASCRFCG *SAWTGGEAPGSCWALWSMPTARSWSLPGTSASF VSSWPRSWCTWQRRGRTTTLTPTRMHSGGA*SR *PPLATGTSTPRPGTAGSLRQPSPSSVSPSSRC LQASWGLGLP*RFRSSTGRSTLRRGGTRQQA*SS RPGDSTPPTSRAQTCTPRGSTTSERSPCPCTVR KLKPTGPPDLSPR*TSWSC*GTSRVNLDSLSGR TPRRSRLQVKAARAEGPCVDAAPDALARRSV*KI VSSPAPEAWLPRGRGPRRPRL*GGHPAPTRASR TAPARCPRAGASGTAAGHARLSASRVPRHGRTQ KKQASPERTLWMTRAAPASL*PRT*PRASKSASE PCVSCGSWCPSGSSRRACGPTT*WTSSSSTQPA TWTCCPELRACSPEWTRSWGGAQRSRTRTAPRA RPRRSCPRTPA*WDGSGRWRSRSCPWRRSWTSW* ISTCSGWASPRQRPRPTLGPKSRSRRRRTTARK TAGSMSTGTAALSRSCAPAAPRARRTSRRPRPR PLSSVRPPPPGSHRATRARATAPPPWGTTAPWCA SRRRLPTSGRCPPTAGATAPAWSSCGRRTPRAA GPPRGTCGTATRPSPSRPWTTRSWSVPSAASAS PSPRRTWMLSTAATRPWRLVPKSGPTLRRESQTP TPTSVPRAGPRHARPPARVPLVTWAGPGPGSEA ALGQWTRPRPSSARCLRGFEAGTLWGPFLTVTE CGGKGGPWRGPCGLKDGGSWQ*PFTKVIFQQGLE GWAGPCGSRSSVQEQGCPVHSAQGRGRHQPGVR RGGSDDGVLPAWQQAGGCLSRAQGRHKGQACSL RTCAKGGPVW*GPAALG

Frame 2

RSEVAAASPRAAQARQECGTAASAMRLPAGGPG LGPAPPPALRPR*A*ARPGAPPARHHGAEVAQR RRIPRPERGEEAEGGLRGAGPRRARLHPGRGAA DRRLRGPQARQHPQQTSRGRRGRREAPQAQRLLP QAAEFPLQRAGAAARLGVHLPRLRVPPGFLLPR AVCVFHHQGV*EELGGGPLHPGNRDYRGVWRGV LRADLGRRLLLPVPWLEGAAQVCPETVLCD*HHG AHRLHCGAGRRLPGQRLCHICAPEPALPADSAD DPHGPAGRHLEAAGLCGLCPQQGAGHCLVHRLP LSHPGLVPGVLGREGGERPL*HLRGCTLVGPDHA DHHWLRGQVPPDLERQAPCGNLHPHRCLLLRAA CRHLGVWVCPEGSGAAQAEAL*EEAEPGSRPDP VGLEILRHQPLAHRPALHVAVLRANGHRAHVQFA NSNLRGLQTYPPAEPAGAAEEPQE*IWTRFQEG PPAGAVSK*RQPVQRAPVWMLPRTL*PEGQFER SCLLQPPRRGCQGEGVPAGPDCEAVTQRRPEPRG QPQQGAQELELRGPQPGTPGFPHQGCRVTAELR RSKPPRRGHCG*QELPLRVCDRGPDPGPQSQHQ SRVCHAVPGVQAEVQGEPAALRRDGRHRAVLSRP PGHAVPN*EPAVQSGPDRGAGPSDHGQGPHQGP GRGGAARGPQHDGTAREGGEAGLVHGEEAGLPG EYLHAADGHPPDRDRGLLWGQRAGAGAAVPQPGR QPGACRQARLHCQDRALQQLHGPEELLGAPGRA PCPVSALHLLAATEPPAPGPRHLPRGGPRLPGA HPAAACPRAVAVRLRRGQPRQHGVPAAGGHPGLQ APRGEPAGQRHVHLHPVRGPRGAGAFLQRLQHL PVQGEPGCSQQLLRGRGALCQSQALHCGGRVRH RLRPLYPVRAPATLGHRRGSLW*RGLGRAQEVRR RWASGPARGPPQHGASEVLRREPSGALFLQ*LS VAGRVGPGGAHVG*RMGAPGSDLLQKLFSNRGW RAGQGPVAPGAACRSKAALSTLLRAAADISPV*G GAGVMTGCCQRGNRRGVVSAEPRGGTKGRPVP* GPAQRAGLFGEDLRPWV

Sequence NM_172106

CGCGGAGCGAGGTGGCCGCAGCGTCTCCGCGCGCGGCCCAAGCCCGGCAGGAGTGCGGAACCGCCGCCTCGGCCATGCGGCTCCCGGCCGGGGGGCCTGG GCTGGGGCCCGCGCCGCCCCCCGCGCTCCGCCCCCGCTGAGCCTGAGCCCGACCCGGGGCGCCTCCCGCCAGGCACCATGGTGCAGAAGTCGCGCAACGG CGGCGTATACCCCGGCCCGAGCGGGGAGAAGAAGCTGAAGGTGGGCTTCGTGGGGCTGGACCCCGGCGCGCCCGACTCCACCCGGGACGGGGCGCTGCTG ATCGCCGGCTCCGAGGCCCCCAAGCGCGGCAGCATCCTCAGCAAACCTCGCGCGGGCGGCGCGGGCGCCGGGAAGCCCCCCAAGCGCAACGCCTTCTACC GCAAGCTGCAGAATTTCCTCTACAACGTGCTGGAGCGGCCGCGCGGCTGGGCGTTCATCTACCACGCCTACGTGTTCCTCCTGGTTTTCTCCTGCCTCGT GCTGTCTGTGTTTTCCACCATCAAGGAGTATGAGAAGAGCTCGGAGGGGGCCCTCTACATCCTGGAAATCGTGACTATCGTGGTGTTTGGCGTGGAGTAC TTCGTGCGGATCTGGGCCGCAGGCTGCTGCTGCCGGTACCGTGGCTGGAGGGGGCGGCTCAAGTTTGCCCGGAAACCGTTCTGTGTGATTGACATCATGG TGCTCATCGCCTCCATTGCGGTGCTGGCCGCCGGCTCCCAGGGCAACGTCTTTGCCACATCTGCGCTCCGGAGCCTGCGCTTCCTGCAGATTCTGCGGAT GATCCGCATGGACCGGCGGGGAGGCACCTGGAAGCTGCTGGGCTCTGTGGTCTATGCCCACAGCAAGGAGCTGGTCACTGCCTGGTACATCGGCTTCCTT TGTCTCATCCTGGCCTCGTTCCTGGTGTACTTGGCAGAGAAGGGGGAGAACGACCACTTTGACACCTACGCGGATGCACTCTGGTGGGGCCTGATCACGC TGACCACCATTGGCTACGGGGACAAGTACCCCCAGACCTGGAACGGCAGGCTCCTTGCGGCAACCTTCACCCTCATCGGTGTCTCCTTCTTCGCGCTGCC TGCAGGCATCTTGGGGTCTGGGTTTGCCCTGAAGGTTCAGGAGCAGCACAGGCAGAAGCACTTTGAGAAGAGGCGGAACCCGGCAGCAGGCCTGATCCAG TCGGCCTGGAGATTCTACGCCACCAACCTCTCGCGCACAGACCTGCACTCCACGTGGCAGTACTACGAGCGAACGGTCACCGTGCCCATGTACAGTTCGC AAACTCAAACCTACGGGGCCTCCAGACTTATCCCCCCGCTGAACCAGCTGGAGCTGCTGAGGAACCTCAAGAGTAAATCTGGACTCGCTTTCAGGAAGGA CCCCCCGCCGGAGCCGTCTCCAAGCCAGAAGGTCAGTTTGAAAGATCGTGTCTTCTCCAGCCCCCGAGGCGTGGCTGCCAAGGGGAAGGGGTCCCCGCAG GCCCAGACTGTGAGGCGGTCACCCAGCGCCGACCAGAGCCTCGAGGACAGCCCCAGCAAGGTGCCCAAGAGCTGGAGCTTCGGGGACCGCAGCCGGGCAC GCCAGGCTTTCCGCATCAAGGGTGCCGCGTCACGGCAGAACTCAGAAGAAGCAAGCCTCCCCGGAGAGGACATTGTGGATGACAAGAGCTGCCCCTGCGA GTTTGTGACCGAGGACCTGACCCCGGGCCTCAAAGTCAGCATCAGAGCCGTGTGTGTCATGCGGTTCCTGGTGTCCAAGCGGAAGTTCAAGGAGAGCCTG CGGCCCTACGACGTGATGGACGTCATCGAGCAGTACTCAGCCGGCCACCTGGACATGCTGTCCCGAATTAAGAGCCTGCAGTCCAGAGTGGACCAGATCG TGGGGCGGGGCCCAGCGATCACGGACAAGGACCGCACCAAGGGCCCGGCCGAGGCGGAGCTGCCCGAGGACCCCAGCATGATGGGACGGCTCGGGAAGGT GGAGAAGCAGGTCTTGTCCATGGAGAAGAAGCTGGACTTCCTGGTGAATATCTACATGCAGCGGATGGGCATCCCCCCGACAGAGACCGAGGCCTACTTT GGGGCCAAAGAGCCGGAGCCGGCGCCGCCGTACCACAGCCCGGAAGACAGCCGGGAGCATGTCGACAGGCACGGCTGCATTGTCAAGATCGTGCGCTCCA GCAGCTCCACGGGCCAGAAGAACTTCTCGGCGCCCCCGGCCGCGCCCCCTGTCCAGTGTCCGCCCTCCACCTCCTGGCAGCCACAGAGCCACCCGCGCCA GGGCCACGGCACCTCCCCCGTGGGGGACCACGGCTCCCTGGTGCGCATCCCGCCGCCGCCTGCCCACGAGCGGTCGCTGTCCGCCTACGGCGGGGGCAAC CGCGCCAGCATGGAGTTCCTGCGGCAGGAGGACACCCCGGGCTGCAGGCCCCCCGAGGGGAACCTGCGGGACAGCGACACGTCCATCTCCATCCCGTCCG TGGACCACGAGGAGCTGGAGCGTTCCTTCAGCGGCTTCAGCATCTCCCAGTCCAAGGAGAACCTGGATGCTCTCAACAGCTGCTACGCGGCCGTGGCGCC TTGTGCCAAAGTCAGGCCCTACATTGCGGAGGGAGAGTCAGACACCGACTCCGACCTCTGTACCCCGTGCGGGCCCCCGCCACGCTCGGCCACCGGCGAG GGTCCCTTTGGTGACGTGGGCTGGGCCGGGCCCAGGAAGTGAGGCGGCGCTGGGCCAGTGGACCCGCCCGCGGCCCTCCTCAGCACGGTGCCTCCGAGGT TTTGAGGCGGGAACCCTCTGGGGCCCTTTTCTTACAGTAACTGAGTGTGGCGGGAAGGGTGGGCCCTGGAGGGGCCCATGTGGGCTGAAGGATGGGGGCT CCTGGCAGTGACCTTTTACAAAAGTTATTTTCCAACAGGGGCTGGAGGGCTGGGCAGGGCCCTGTGGCTCCAGGAGCAGCGTGCAGGAGCAAGGCTGCCC TGTCCACTCTGCTCAGGGCCGCGGCCGACATCAGCCCGGTGTGAGGAGGGGCGGGAGTGATGACGGGGTGTTGCCAGCGTGGCAACAGGCGGGGGGTTGT CTCAGCCGAGCCCAGGGGAGGCACAAAGGGCAGGCCTGTTCCCTGAGGACCTGCGCAAAGGGCGGGCCTGTTTGGTGAGGACCTGCGGCCTTGGGTC

refseq_KCNQ2.F1 (forward primer)
refseq_KCNQ2.R1 (reverse primer)
Single exon skipping

Frame 0

RGARWPQRLRARPKPGRSAEPPPRPCGSRPGGL GWGPRRPPRSAPAEPEPDPGRLPPGTMVQKSRNG GVYPGPSGEKKLKVGFVGLDPGAPDSTRDGALL IAGSEAPKRGSILSKPRAGGAGAGKPPKRNAFY RKLQNFLYNVLERPRGWAFIYHAYVFLLVFSCLV LSVFSTIKEYEKSSEGALYILEIVTIVVFGVEY FVRIWAAGCCCRYRGWRGRLKFARKPFCVIDIM VLIASIAVLAAGSQGNVFATSALRSLRFLQILRM IRMDRRGGTWKLLGSVVYAHSKELVTAWYIGFL CLILASFLVYLAEKGENDHFDTYADALWWGLIT LTTIGYGDKYPQTWNGRLLAATFTLIGVSFFALP AGILGSGFALKVQEQHRQKHFEKRRNPAAGLIQ SAWRFYATNLSRTDLHSTWQYYERTVTVPMYSS QTQTYGASRLIPPLNQLELLRNLKSKSGLAFRKD PPPEPSPSQKVSLKDRVFSSPRGVAAKGKGSPQ AQTVRRSPSADQSLEDSPSKVPKSWSFGDRSRA RQAFRIKGAASRQNSEEASLPGEDIVDDKSCPCE FVTEDLTPGLKVSIRAVCVMRFLVSKRKFKESL RPYDVMDVIEQYSAGHLDMLSRIKSLQSRVDQI VGRGPAITDKDRTKGPAEAELPEDPSMMGRLGKV EKQVLSMEKKLDFLVNIYMQRMGIPPTETEAYF GAKEPEPAPPYHSPEDSREHVDRHGCIVKIVRS SSSTGQKNFSAPPAAPPVQCPPSTSWQPQSHPRQ GHGTSPVGDHGSLVRIPPPPAHERSLSAYGGGN RASMEFLRQEDTPGCRPPEGNLRDSDTSISIPS VDHEELERSFSGFSISQSKENLDALNSCYAAVAP CAKVRPYIAEGESDTDSDLCTPCGPPPRSATGE GPFGDVGWAGPRK*GGAGPVDPPAALLSTVPPR F*GGNPLGPFSYSN*VWREGWALEGPMWAEGWGL LAVTFYKSYFPTGAGGLGRALWLQEQRAGARLP CPLCSGPRPTSARCEEGRE**RGVASVATGGGL SQPSPGEAQRAGLFPEDLRKGRACLVRTCGLG

Frame 1

AERGGRSVSARGPSPAGVRNRRLGHAAPGRGAW AGARAAPRAPPPLSLSPTRGASRQAPWCRSRAT AAYTPARAGRRS*RWASWGWTPARPTPPGTGRC* SPAPRPPSAAASSANLARAARAPGSPPSATPST ASCRISSTTCWSGRAAGRSSTTPTCSSWFSPAS CCLCFPPSRSMRRARRGPSTSWKS*LSWCLAWST SCGSGPQAAAAGTVAGGGGSSLPGNRSV*LTSW CSSPPLRCWPPAPRATSLPHLRSGACASCRFCG *SAWTGGEAPGSCWALWSMPTARSWSLPGTSASF VSSWPRSWCTWQRRGRTTTLTPTRMHSGGA*SR *PPLATGTSTPRPGTAGSLRQPSPSSVSPSSRC LQASWGLGLP*RFRSSTGRSTLRRGGTRQQA*SS RPGDSTPPTSRAQTCTPRGSTTSERSPCPCTVR KLKPTGPPDLSPR*TSWSC*GTSRVNLDSLSGR TPRRSRLQARRSV*KIVSSPAPEAWLPRGRGPRR PRL*GGHPAPTRASRTAPARCPRAGASGTAAGH ARLSASRVPRHGRTQKKQASPERTLWMTRAAPA SL*PRT*PRASKSASEPCVSCGSWCPSGSSRRAC GPTT*WTSSSSTQPATWTCCPELRACSPEWTRS WGGAQRSRTRTAPRARPRRSCPRTPA*WDGSGR WRSRSCPWRRSWTSW*ISTCSGWASPRQRPRPTL GPKSRSRRRRTTARKTAGSMSTGTAALSRSCAP AAPRARRTSRRPRPRPLSSVRPPPPGSHRATRA RATAPPPWGTTAPWCASRRRLPTSGRCPPTAGAT APAWSSCGRRTPRAAGPPRGTCGTATRPSPSRP WTTRSWSVPSAASASPSPRRTWMLSTAATRPWR LVPKSGPTLRRESQTPTPTSVPRAGPRHARPPAR VPLVTWAGPGPGSEAALGQWTRPRPSSARCLRG FEAGTLWGPFLTVTECGGKGGPWRGPCGLKDGG SWQ*PFTKVIFQQGLEGWAGPCGSRSSVQEQGCP VHSAQGRGRHQPGVRRGGSDDGVLPAWQQAGGC LSRAQGRHKGQACSLRTCAKGGPVW*GPAALG

Frame 2

RSEVAAASPRAAQARQECGTAASAMRLPAGGPG LGPAPPPALRPR*A*ARPGAPPARHHGAEVAQR RRIPRPERGEEAEGGLRGAGPRRARLHPGRGAA DRRLRGPQARQHPQQTSRGRRGRREAPQAQRLLP QAAEFPLQRAGAAARLGVHLPRLRVPPGFLLPR AVCVFHHQGV*EELGGGPLHPGNRDYRGVWRGV LRADLGRRLLLPVPWLEGAAQVCPETVLCD*HHG AHRLHCGAGRRLPGQRLCHICAPEPALPADSAD DPHGPAGRHLEAAGLCGLCPQQGAGHCLVHRLP LSHPGLVPGVLGREGGERPL*HLRGCTLVGPDHA DHHWLRGQVPPDLERQAPCGNLHPHRCLLLRAA CRHLGVWVCPEGSGAAQAEAL*EEAEPGSRPDP VGLEILRHQPLAHRPALHVAVLRANGHRAHVQFA NSNLRGLQTYPPAEPAGAAEEPQE*IWTRFQEG PPAGAVSKPEGQFERSCLLQPPRRGCQGEGVPA GPDCEAVTQRRPEPRGQPQQGAQELELRGPQPGT PGFPHQGCRVTAELRRSKPPRRGHCG*QELPLR VCDRGPDPGPQSQHQSRVCHAVPGVQAEVQGEP AALRRDGRHRAVLSRPPGHAVPN*EPAVQSGPDR GAGPSDHGQGPHQGPGRGGAARGPQHDGTAREG GEAGLVHGEEAGLPGEYLHAADGHPPDRDRGLL WGQRAGAGAAVPQPGRQPGACRQARLHCQDRALQ QLHGPEELLGAPGRAPCPVSALHLLAATEPPAP GPRHLPRGGPRLPGAHPAAACPRAVAVRLRRGQ PRQHGVPAAGGHPGLQAPRGEPAGQRHVHLHPVR GPRGAGAFLQRLQHLPVQGEPGCSQQLLRGRGA LCQSQALHCGGRVRHRLRPLYPVRAPATLGHRR GSLW*RGLGRAQEVRRRWASGPARGPPQHGASEV LRREPSGALFLQ*LSVAGRVGPGGAHVG*RMGA PGSDLLQKLFSNRGWRAGQGPVAPGAACRSKAA LSTLLRAAADISPV*GGAGVMTGCCQRGNRRGVV SAEPRGGTKGRPVP*GPAQRAGLFGEDLRPWV