Back to Rnomics Platform
Open Session
×
1 warning:
- Access restricted to public data.
Please sign in at Rnomics Platform to access your data.
Sequence NM_207293
GTTGGTACTAAGAAGTGCCTTTCCTGACGTCTCTGCTGCTTGGAACCGCTTCTAGAGCAGTCTCTGCTTTTGCCTTGCTTGCTGCCAGCTAGACTGTGACGACAGCACATCCACCCTCCACCTCTAGCCCAGACACCCCCATTTCTACTTATAATCAAGAGAAAAGCTCTAAGTATCTGGCATTGCCCTAGGCTGCTTTA
GTGTTAAAAGAAAAGTTTGCTGAAAAAGTAAGATATCTTCTGCCAGGAAATCAAGGAGGAAAAAAAAAATCATTTTCTCGATTTTGCTCTAAACTGCTGC
ATCTGTCTATGCCAAACTAATCAATACCGATTGCACCACCAAACTCCATTGCAAATTCAGCTGTGAGGAGATTCCCTTTCAGACAACTTTGCTGAAAGCA
GCTTGGAAATTCGGTGTCGAAGGGTCTGCCACGTTTTCATGCTTGCATTTTGGGCTCCAAATTGGCACTGGGAAGGGGTTACTGAGAGCACAAGGCTGAT
ACCAGGCCCTACTTTTAAACGTTCATCTACTTACAATCCTAGTATTTCTCTAAAAACCAAAACCTCTTTGAATTAACAGTTTCATGCTGTGAATTTCTAG
TGGGAGATCTTTTCCTTGATATTGACGACACAATTTTCCATGTACTTTTAAAGCAGGGAGTGGGGAAAAGTATTTTGAGGGGACATTTTCATCATCAGTT
CAGCTTTTTTTTTTTGGTTGTTGCTCTTTTTTGGGGGGGTTGGGTTTGTTGGTTTCACTGAAACATTTAACTACCTGTAAAATCTAAACATGGCTGTTAG
TGTCACACCAATTCGGGACACAAAATGGCTAACACTGGAAGTATGTAGAGAGTTCCAGAGGGGGACTTGCTCACGGCCAGACACGGAATGTAAATTTGCA
CATCCTTCGAAAAGCTGCCAAGTTGAAAATGGACGAGTAATCGCCTGCTTTGATTCATTGAAAGGCCGTTGCTCCAGGGAGAACTGCAAATATCTTCATC
CACCCCCACATTTAAAAACGCAGTTGGAGATAAATGGACGCAATAACTTGATTCAGCAGAAGAACATGGCCATGTTGGCCCAGCAAATGCAACTAGCCAA
TGCCATGATGCCTGGTGCCCCATTACAACCCGTGCCAATGTTTTCAGTTGCACCAAGCTTAGCCACCAATGCATCAGCAGCCGCCTTTAATCCCTATCTG
GGACCTGTTTCTCCAAGCCTGGTCCCGGCAGAGATCTTGCCGACTGCACCAATGTTGGTTACAGGGAATCCGGGTGTCCCTGTACCTGCAGCTGCTGCAG
CTGCTGCACAGAAATTAATGCGAACAGACAGACTTGAGGTATGTCGAGAGTACCAACGTGGCAATTGCAACCGAGGAGAAAATGATTGTCGGTTTGCTCA
TCCTGCTGACAGCACAATGATTGACACCAATGACAACACAGTCACTGTGTGTATGGATTACATCAAAGGGAGATGCTCTCGGGAAAAGTGCAAATACTTT
CATCCCCCTGCACATTTGCAAGCCAAGATCAAGGCTGCCCAATACCAGGTCAACCAGGCTGCAGCTGCACAGGCTGCAGCCACCGCAGCTGCCATGACTC
AGTCGGCTGTCAAATCACTGAAGCGACCCCTCGAGGCAACCTTTGACCTGGGAATTCCTCAAGCTGTACTTCCCCCATTACCAAAGAGGCCTGCTCTTGA
AAAAACCAACGGTGCCACCGCAGTCTTTAACACTGGTATTTTCCAATACCAACAGGCTCTAGCCAACATGCAGTTACAACAGCATACAGCATTTCTCCCA
CCAGTTCCCATGGTGCACGGTGCTACGCCAGCCACTGTGTCCGCAGCAACAACATCTGCCACAAGTGTTCCCTTCGCTGCAACAGCCACAGCCAACCAGA
TACCCATAATATCTGCCGAACATCTGACTAGCCACAAGTATGTTACCCAGATGTAGAATTTTCATCACTAAACAATCATGCTAAAGAGGAAAGGACAGTG
TGCTTGGTTAGAGTAAAGGACGAGGTCATTAGCCATATTGTATATATCGTCAAGCAACACACACAAAAGTTCCTCAGCCACAAGACATCCACATATTGCA
TGTTAACCAGAAGAAAAGACAACATTTTCCGGAAATCCACTGCACACTGTTGCCTATACACTTTGTACATTTAATTGATATTTGTGCTGAGGTGATATTC
CTGTCTAAAAGAACAACATTGTCTTTCTTTTCTAGCACAGAGTTATGCATTCAAAGATGCATACCTAGTTAGTTTCCTATATATTCATGCCATCTTGAAA
AGACAGACTATGGTGTAACCATGATTCTATTATGTATTGGTACGTCTGTAGACCAAGATATAATTTTTTAAAAATAAGTTTATTTCTTTCAAGGTTTACA
AATAACAAAGGTGCACCTTGTATTTAAAATTGCCATTATAGATGAGAGCGTGCATGCACAGTCATTTTTGTTTAAGAGTAATATTTTTAATGTAATAGAT
TGTAAGACGTGGTGAGGGAGGGATCTGACAGAGATGAATGTGCCAAGCAAAACCACAACTGTGTATATTTTAAAGCACATCATGGCTTTAAGTACCATGT
TGTTAAGGATTCTCATGAAGTGCCATAGACTGTACATCAAATTAGAGTATTATTTCTTCAGTGTTATTGTTTTCAGAGCCACATTTTGTTGCATATTTGC
TAGTACTAATCAGTCAAAGGGCACCATTCTTTTTTTTTTTTTTTGAAACCAAAGCTGTCTCAGAAATGGCCAATTTAACTTTACAGTAACAATAGACAGC
ACAACACAAACTCTCTCAATACAGATAAACTCACACATACTGGAGATATATATATAATAGATATATATAAAATTATTTTAATGCATTGTAGTGTAATATT
TATGCATACTATACTGTATAACATGTTATTCAAAAGGGATTGCCATTTCTGAGACACAGTAACAAAAAAATGAGGAAATTATTTTGCTTCTATTTATAGC
CTCTGTCAAAAGTCAAAAGACTATAAATGCTTTGCAAAAATGGTTTCACGTTTGCTTAAATGCTTCATCACAGTCACATTCAAAATAGTGACTCTAAACA
AAGAAGAAAGCAGCACTGTCATCAGATGCATGATAAACCAAAATATGAAAATGGGAAATGTTTAATTAACCTAGTAATTGGGTGGGTTAAGTACATGGGT
GAATTTTATATGTGATTTTTGTTTTGTTTTGTTTTGTTCAGATTAACTGCTTATAGCCTTAGAAAGCCTTTTACAAAATTAAAAAAAAAATAGATGTGCA
TTCAGTTTTTAAGAATGGAATCATCCAAAGGAATTCCTTTTTTTGAGGTTTGGATGTTGCAGCTAGTAAAGGATATTTTTGCTCTGTTCAGCAGTTCTAA
AAATTGCTGAAGTAGGGGCCAGGTCACTGGTAGTTATAGTATGGAATGGGAGAAGTGAAAGTTCAGTTATAGAACTTTCCATACTTCCAAGTTTACTGCA
AGTTTTTATGCTTGAGAGAGATGCTTTCTAATATAAGACTGATGTGTTGATTTTACTGATTGTACTGTACATCTATTAAAGCCTTAGATTATTACATTAC
GGGTTGGAACCCATACCAATGTAATTTCAATCGTGTTAAGAAAGTAATGGTGACTTCACATGTTATTGTAGTTAGTTACATTATAGAATATTACTTATTT
TTCTTGTTAAAATGTAGTTTTTCATTTCCTACATTTATTAGATTTTCATTTTCTATTAACAATTGAATACCATTTCAGTTTATAGACTTGTTTTATTAGA
TTTTACCAATGAATTTTTCAAAATACAAAAAAAAGTAGTTTTTCCTTCATAACATACTCAGTTTTGAATTACATGTAGTGTCACATGAATATTCGTATTG
TTAACTAAATGATTTATATTTTACTGATTTAATATTACAGTGTAAGAATGTCAGTCATTGTTAGTTCTTGTCTAGTTTTCATTAAAAGAACAAAGATCTT
TTATATGGATATCTTATAAATATATAATCATTGCTAAGTAAGAAGTTAAGTTGTTGCTATCGCAACAATCCTGGCAGACAATTGAGTAATATTTTGATGA
TTTATTTTGTTTGTAATTAGTTATTATAAGAAGATCTAGATCCTAGATATTAGAATAAAATTTATTTTCTACTGTATCCATTTCAAATGTTAAAATATTG
TTTAATATTTTTGAAATCCCTGAGTATCAGGCCTTGTTATAAATAAGCTGCATAATCAATAAATAGAACAAGGGACTTTTTGTTGATAATCCAAATACTC
AAAGTTTACGTAATGAAAATTATAGCGTGTGTGCAAACTCTTGAGGGTTGATTATGCTGCAATTTAGCATGTTGGAACGTCTAGGGAGAAGGTTGACTTT
TTGCACTTCTGTATATAGTCAAAAGAGAGAAACCTGTATAATAGTAAGATCTTATTTTGAATAAAAACGTCTATAATTACAAGGAGTTTTGTTAAGGCTA
ATACAATGACAGACTGAGCAAAATTGCTTGCAAAAGTGGCACAGAGTTAGCACTCCATACCCCTTCAAACATGTTGCTTTGCTTTCTTGTGGACAGCTTG
TAGTTTGCCAGGATTTTTTCAGCTGGAAAGATACGCCATCCTTTCAAACCCTCATGACTGACAAAAACTCCATGGGGCCAAATCTGCCTGAAGATCATTA
CCAAAAATAGCAGGTACTTCTACCATTAAGGTGAAATCATGGATCAGATATTCCTTACATTTTTCAAAACTACTGCATGTTTAAAACTTCAACAAAAAAA
GAGAGAAAGAACTATACTAAGAACATATATTATTCAGATCAGTTTCTGCCAATTTCAGTGGTTTATTGTTCACAAAAAAATCTTCAAAACAAGTATTGAC
TTTCACAAAATTTAAATCATAAACAGGCAAACCAAACAGCACACTGTAGCTATAGTTGTTATGTGATTGTTTTTTAATTGCTGTAGGATCCTGTTCTTTC
AGCAGGTGAAAAATAAAACGCAGTTCAAATTTCATGGTTTTAATTTTCAACTCAGAAGCACTCAAAAATGCAAAATGTGATAATGGGCACTTGTTTAAAA
GAATTAGTGTATCCAGCCTTCACTCCAGCTGGTTAAAAATGTTGCACTTATCAGCAACCCTACCACTTTCATCTGCTGAAAGGACAAATGTGCTTGGTTT
TACTATTATGTAATCACAACTTACTTTCTGCTTGTAGTTGCTTAAAATTATGTATTTTGTCTTGGGCTGCAATTTGTTTTATGCTTATTTTATTATTACT
GCAGTAGTTGACTTTGCTGTATGGAAAAATAAAGTGAAATTGCCCTAATAAAACTTCTCTTTCTTAAGTA
- refseq_MBNL1.F2 (forward primer)
- refseq_MBNL1.R2 (reverse primer)
- Single exon skipping
Frame 0
VGTKKCLS*RLCCLEPLLEQSLLLPCLLPARL*RQHIHPPPLAQTPPFLLIIKRKALSIWHCPRLL*
C*KKSLLKK*DIFCQEIKEEKKNHFLDFALNCC
ICLCQTNQYRLHHQTPLQIQL*GDSLSDNFAES
SLEIRCRRVCHVFMLAFWAPNWHWEGVTESTRLI
PGPTFKRSSTYNPSISLKTKTSLN*QFHAVNF*
WEIFSLILTTQFSMYF*SREWGKVF*GDIFIIS
SAFFFWLLLFFGGVGFVGFTETFNYL*NLNMAVS
VTPIRDTKWLTLEVCREFQRGTCSRPDTECKFA
HPSKSCQVENGRVIACFDSLKGRCSRENCKYLH
PPPHLKTQLEINGRNNLIQQKNMAMLAQQMQLAN
AMMPGAPLQPVPMFSVAPSLATNASAAAFNPYL
GPVSPSLVPAEILPTAPMLVTGNPGVPVPAAAA
AAAQKLMRTDRLEVCREYQRGNCNRGENDCRFAH
PADSTMIDTNDNTVTVCMDYIKGRCSREKCKYF
HPPAHLQAKIKAAQYQVNQAAAAQAAATAAAMT
QSAVKSLKRPLEATFDLGIPQAVLPPLPKRPALE
KTNGATAVFNTGIFQYQQALANMQLQQHTAFLP
PVPMVHGATPATVSAATTSATSVPFAATATANQ
IPIISAEHLTSHKYVTQM*NFHH*TIMLKRKGQC
AWLE*RTRSLAILYISSSNTHKSSSATRHPHIA
C*PEEKTTFSGNPLHTVAYTLCTFN*YLC*GDI
PV*KNNIVFLF*HRVMHSKMHT*LVSYIFMPS*K
DRLWCNHDSIMYWYVCRPRYNFLKISLFLSRFT
NNKGAPCI*NCHYR*ERACTVIFV*E*YF*CNR
L*DVVREGSDRDECAKQNHNCVYFKAHHGFKYHV
VKDSHEVP*TVHQIRVLFLQCYCFQSHILLHIC
*Y*SVKGHHSFFFFLKPKLSQKWPI*LYSNNRQ
HNTNSLNTDKLTHTGDIYIIDIYKIILMHCSVIF
MHTILYNMLFKRDCHF*DTVTKK*GNYFASIYS
LCQKSKDYKCFAKMVSRLLKCFITVTFKIVTLN
KEESSTVIRCMINQNMKMGNV*LT**LGGLSTWV
NFICDFCFVLFCSD*LLIALESLLQN*KKNRCA
FSF*EWNHPKEFLFLRFGCCS**RIFLLCSAVL
KIAEVGARSLVVIVWNGRSESSVIELSILPSLLQ
VFMLERDAF*YKTDVLILLIVLYIY*SLRLLHY
GLEPIPM*FQSC*ESNGDFTCYCS*LHYRILLI
FLVKM*FFISYIY*IFIFY*QLNTISVYRLVLLD
FTNEFFKIQKKVVFPS*HTQF*ITCSVT*IFVL
LTK*FIFY*FNITV*ECQSLLVLV*FSLKEQRS
FIWISYKYIIIAK*EVKLLLSQQSWQTIE*YFDD
LFCL*LVIIRRSRS*ILE*NLFSTVSISNVKIL
FNIFEIPEYQALL*ISCIINK*NKGLFVDNPNT
QSLRNENYSVCANS*GLIMLQFSMLERLGRRLTF
CTSVYSQKRETCIIVRSYFE*KRL*LQGVLLRL
IQ*QTEQNCLQKWHRVSTPYPFKHVALLSCGQL
VVCQDFFSWKDTPSFQTLMTDKNSMGPNLPEDHY
QK*QVLLPLR*NHGSDIPYIFQNYCMFKTSTKK
ERKNYTKNIYYSDQFLPISVVYCSQKNLQNKY*
LSQNLNHKQANQTAHCSYSCYVIVF*LL*DPVLS
AGEK*NAVQISWF*FSTQKHSKMQNVIMGTCLK
ELVYPAFTPAG*KCCTYQQPYHFHLLKGQMCLV
LLLCNHNLLSACSCLKLCILSWAAICFMLILLLL
Q*LTLLYGKIK*NCPNKTSLS*V
Frame 1
LVLRSAFPDVSAAWNRF*SSLCFCLACCQLDCDDSTSTLHL*PRHPHFYL*SREKL*VSGIALGCF
SVKRKVC*KSKISSARKSRRKKKIIFSILL*TAA
SVYAKLINTDCTTKLHCKFSCEEIPFQTTLLKA
AWKFGVEGSATFSCLHFGLQIGTGKGLLRAQG*
YQALLLNVHLLTILVFL*KPKPL*INSFML*ISS
GRSFP*Y*RHNFPCTFKAGSGEKYFEGTFSSSV
QLFFFGCCSFLGGLGLLVSLKHLTTCKI*TWLL
VSHQFGTQNG*HWKYVESSRGGLAHGQTRNVNLH
ILRKAAKLKMDE*SPALIH*KAVAPGRTANIFI
HPHI*KRSWR*MDAIT*FSRRTWPCWPSKCN*P
MP*CLVPHYNPCQCFQLHQA*PPMHQQPPLIPIW
DLFLQAWSRQRSCRLHQCWLQGIRVSLYLQLLQ
LLHRN*CEQTDLRYVESTNVAIATEEKMIVGLL
ILLTAQ*LTPMTTQSLCVWITSKGDALGKSANTF
IPLHICKPRSRLPNTRSTRLQLHRLQPPQLP*L
SRLSNH*SDPSRQPLTWEFLKLYFPHYQRGLLL
KKPTVPPQSLTLVFSNTNRL*PTCSYNSIQHFSH
QFPWCTVLRQPLCPQQQHLPQVFPSLQQPQPTR
YP*YLPNI*LATSMLPRCRIFITKQSC*RGKDS
VLG*SKGRGH*PYCIYRQATHTKVPQPQDIHILH
VNQKKRQHFPEIHCTLLPIHFVHLIDICAEVIF
LSKRTTLSFFSSTELCIQRCIPS*FPIYSCHLE
KTDYGVTMILLCIGTSVDQDIIF*K*VYFFQGLQ
ITKVHLVFKIAIIDESVHAQSFLFKSNIFNVID
CKTW*GRDLTEMNVPSKTTTVYILKHIMALSTM
LLRILMKCHRLYIKLEYYFFSVIVFRATFCCIFA
STNQSKGTILFFFF*NQSCLRNGQFNFTVTIDS
TTQTLSIQINSHILEIYI**IYIKLF*CIVV*Y
LCILYCITCYSKGIAISETQ*QKNEEIILLLFIA
SVKSQKTINALQKWFHVCLNASSQSHSK**L*T
KKKAALSSDA**TKI*KWEMFN*PSNWVG*VHG
*ILYVIFVLFCFVQINCL*P*KAFYKIKKKIDVH
SVFKNGIIQRNSFF*GLDVAASKGYFCSVQQF*
KLLK*GPGHW*L*YGMGEVKVQL*NFPYFQVYC
KFLCLREMLSNIRLMC*FY*LYCTSIKALDYYIT
GWNPYQCNFNRVKKVMVTSHVIVVSYIIEYYLF
FLLKCSFSFPTFIRFSFSINN*IPFQFIDLFY*
ILPMNFSKYKKK*FFLHNILSFELHVVSHEYSYC
*LNDLYFTDLILQCKNVSHC*FLSSFH*KNKDL
LYGYLINI*SLLSKKLSCCYRNNPGRQLSNILM
IYFVCN*LL*EDLDPRY*NKIYFLLYPFQMLKYC
LIFLKSLSIRPCYK*AA*SINRTRDFLLIIQIL
KVYVMKIIACVQTLEG*LCCNLACWNV*GEG*L
FALLYIVKREKPV***DLILNKNVYNYKEFC*G*
YNDRLSKIACKSGTELALHTPSNMLLCFLVDSL
*FARIFSAGKIRHPFKPS*LTKTPWGQICLKII
TKNSRYFYH*GEIMDQIFLTFFKTTACLKLQQKK
RERTILRTYIIQISFCQFQWFIVHKKIFKTSID
FHKI*IINRQTKQHTVAIVVM*LFFNCCRILFF
QQVKNKTQFKFHGFNFQLRSTQKCKM**WALV*K
N*CIQPSLQLVKNVALISNPTTFIC*KDKCAWF
YYYVITTYFLLVVA*NYVFCLGLQFVLCLFYYY
CSS*LCCMEK*SEIALIKLLFLK
Frame 2
WY*EVPFLTSLLLGTASRAVSAFALLAAS*TVTTAHPPSTSSPDTPISTYNQEKSSKYLALP*AAL
VLKEKFAEKVRYLLPGNQGGKKKSFSRFCSKLL
HLSMPN*SIPIAPPNSIANSAVRRFPFRQLC*KQ
LGNSVSKGLPRFHACILGSKLALGRGY*EHKAD
TRPYF*TFIYLQS*YFSKNQNLFELTVSCCEFL
VGDLFLDIDDTIFHVLLKQGVGKSILRGHFHHQF
SFFFLVVALFWGGWVCWFH*NI*LPVKSKHGC*
CHTNSGHKMANTGSM*RVPEGDLLTARHGM*IC
TSFEKLPS*KWTSNRLL*FIERPLLQGELQISSS
TPTFKNAVGDKWTQ*LDSAEEHGHVGPANATSQ
CHDAWCPITTRANVFSCTKLSHQCISSRL*SLS
GTCFSKPGPGRDLADCTNVGYRESGCPCTCSCCS
CCTEINANRQT*GMSRVPTWQLQPRRK*LSVCS
SC*QHND*HQ*QHSHCVYGLHQREMLSGKVQIL
SSPCTFASQDQGCPIPGQPGCSCTGCSHRSCHDS
VGCQITEATPRGNL*PGNSSSCTSPITKEACS*
KNQRCHRSL*HWYFPIPTGSSQHAVTTAYSISP
TSSHGARCYASHCVRSNNICHKCSLRCNSHSQPD
THNICRTSD*PQVCYPDVEFSSLNNHAKEERTV
CLVRVKDEVISHIVYIVKQHTQKFLSHKTSTYC
MLTRRKDNIFRKSTAHCCLYTLYI*LIFVLR*YS
CLKEQHCLSFLAQSYAFKDAYLVSFLYIHAILK
RQTMV*P*FYYVLVRL*TKI*FFKNKFISFKVY
K*QRCTLYLKLPL*MRACMHSHFCLRVIFLM**I
VRRGEGGI*QR*MCQAKPQLCIF*STSWL*VPC
C*GFS*SAIDCTSN*SIISSVLLFSEPHFVAYL
LVLISQRAPFFFFFFETKAVSEMANLTLQ*Q*TA
QHKLSQYR*THTYWRYIYNRYI*NYFNAL*CNI
YAYYTV*HVIQKGLPFLRHSNKKMRKLFCFYL*
PLSKVKRL*MLCKNGFTFA*MLHHSHIQNSDSKQ
RRKQHCHQMHDKPKYENGKCLINLVIGWVKYMG
EFYM*FLFCFVLFRLTAYSLRKPFTKLKKK*MC
IQFLRMESSKGIPFFEVWMLQLVKDIFALFSSSK
NC*SRGQVTGSYSMEWEK*KFSYRTFHTSKFTA
SFYA*ERCFLI*D*CVDFTDCTVHLLKP*IITL
RVGTHTNVISIVLRK*W*LHMLL*LVTL*NITYF
SC*NVVFHFLHLLDFHFLLTIEYHFSL*TCFIR
FYQ*IFQNTKKSSFSFITYSVLNYM*CHMNIRI
VN*MIYILLI*YYSVRMSVIVSSCLVFIKRTKIF
YMDIL*IYNHC*VRS*VVAIATILADN*VIF**
FILFVISYYKKI*ILDIRIKFIFYCIHFKC*NI
V*YF*NP*VSGLVINKLHNQ*IEQGTFC**SKYS
KFT**KL*RVCKLLRVDYAAI*HVGTSREKVDF
LHFCI*SKERNLYNSKILF*IKTSIITRSFVKA
NTMTD*AKLLAKVAQS*HSIPLQTCCFAFLWTAC
SLPGFFQLERYAILSNPHD*QKLHGAKSA*RSL
PKIAGTSTIKVKSWIRYSLHFSKLLHV*NFNKK
REKELY*EHILFRSVSANFSGLLFTKKSSKQVLT
FTKFKS*TGKPNSTL*L*LLCDCFLIAVGSCSF
SR*KIKRSSNFMVLIFNSEALKNAKCDNGHLFK
RISVSSLHSSWLKMLHLSATLPLSSAERTNVLGF
TIM*SQLTFCL*LLKIMYFVLGCNLFYAYFIIT
AVVDFAVWKNKVKLP**NFSFLS
Sequence NM_207292
TTACATCTTATAGCACCCAGTGAATTATGATATGGGGCTGCGGTTTATTTGCTCTGGCGACAATATGGCTTACTTGCCGTGGAATATCCCGGTATTTCAGAAGTAACATATTATTTTTAAAGAGGGAGCTTTGCTTTCTCTAAATCACATGGTGCAAAGTTGGGAATACAGTGGCCTGTAAATTACAAATATTATTTACA
AACTAGAAGGAAAGCTGACATGTGTTTGTGCCTGTGTGACAGTTTCACTAAGGAATTCCAAAACTGTGGATAGCCTGTTACTTGAGAAAAATACACGGTG
GCAGTCTTGCAGATGTTTTTCCCGCCTGTTAGCCCTCCAGGTTCAAAAACTGTTTGGTGCATTTTAAGGCGGCAGTCTCACCAGATCGCAGGATTCTAGT
CTAATTGGCTTCTAACCTTCAATCTAACCTTGCCGGCTTCCTTGCAAAGCCCGGTGCAAGGGCCTCTTTCAAAATGAACCCACTGGTGTGCCTAGCAGTC
GGTAGAAGAAGCGGGAGGGCGTCCGGTCTGCACGCCCGCCGCGAGGTTACAATGCTGAACGCATGAGATGGAAGATACCAACGGGAGGCCGAGGGGATCC
ACGGCGCCCGCGCGGGCTCCGGCTTCCTCCTGCTCTCGGCGCCGCTGGGCGACCGCCCATGACCCGCTCTTGCGGGCTCTGTCCGGTTGACAGGCGACCC
TGTGGCCCGGGGAAGCGCGGGAGGGCGCCGGCGGAAAGTTGAAGAGCGTTTTTCTCGCCGCCGCGTGCATTAGGAGCTCGACGAGTCCGCCCTGGGCTTC
CTGGTGGGGCTGGGCGGGCGGGGGAGGGGCCGCGCAGCAGCAGCGGAAGCCAGACCTCGGCGATAAGAGGCTGCACAGCGACATGCAACAGTCTTTTCAC
TGCAGCTGAATGAGTTGTGGCGCCCACAATGCTCCCATGACAAGGAGCTGACAAGTTCCATTTTCCGTCGCGGGCATCTTGGAATCATGACTCCCACAAT
GCCTTGGGCACTTGGTCGACAGTGGGGCCGCCTCTGAAAAAAAAATGTGAGAGGTTGGTACTAAGAAGTGCCTTTCCTGACGTCTCTGCTGCTTGGAACC
GCTTCTAGAGCAGTCTCTGCTTTTGCCTTGCTTGCTGCCAGCTAGACTGTGACGACAGCACATCCACCCTCCACCTCTAGCCCAGACACCCCCATTTCTA
CTTATAATCAAGAGAAAAGCTCTAAGTATCTGGCATTGCCCTAGGCTGCTTTAGTGTTAAAAGAAAAGTTTGCTGAAAAAGTAAGATATCTTCTGCCAGG
AAATCAAGGAGGAAAAAAAAAATCATTTTCTCGATTTTGCTCTAAACTGCTGCATCTGTCTATGCCAAACTAATCAATACCGATTGCACCACCAAACTCC
ATTGCAAATTCAGCTGTGAGGAGATTCCCTTTCAGACAACTTTGCTGAAAGCAGCTTGGAAATTCGGTGTCGAAGGGTCTGCCACGTTTTCATGCTTGCA
TTTTGGGCTCCAAATTGGCACTGGGAAGGGGTTACTGAGAGCACAAGGCTGATACCAGGCCCTACTTTTAAACGTTCATCTACTTACAATCCTAGTATTT
CTCTAAAAACCAAAACCTCTTTGAATTAACAGTTTCATGCTGTGAATTTCTAGTGGGAGATCTTTTCCTTGATATTGACGACACAATTTTCCATGTACTT
TTAAAGCAGGGAGTGGGGAAAAGTATTTTGAGGGGACATTTTCATCATCAGTTCAGCTTTTTTTTTTTGGTTGTTGCTCTTTTTTGGGGGGGTTGGGTTT
GTTGGTTTCACTGAAACATTTAACTACCTGTAAAATCTAAACATGGCTGTTAGTGTCACACCAATTCGGGACACAAAATGGCTAACACTGGAAGTATGTA
GAGAGTTCCAGAGGGGGACTTGCTCACGGCCAGACACGGAATGTAAATTTGCACATCCTTCGAAAAGCTGCCAAGTTGAAAATGGACGAGTAATCGCCTG
CTTTGATTCATTGAAAGGCCGTTGCTCCAGGGAGAACTGCAAATATCTTCATCCACCCCCACATTTAAAAACGCAGTTGGAGATAAATGGACGCAATAAC
TTGATTCAGCAGAAGAACATGGCCATGTTGGCCCAGCAAATGCAACTAGCCAATGCCATGATGCCTGGTGCCCCATTACAACCCGTGCCAATGTTTTCAG
TTGCACCAAGCTTAGCCACCAATGCATCAGCAGCCGCCTTTAATCCCTATCTGGGACCTGTTTCTCCAAGCCTGGTCCCGGCAGAGATCTTGCCGACTGC
ACCAATGTTGGTTACAGGGAATCCGGGTGTCCCTGTACCTGCAGCTGCTGCAGCTGCTGCACAGAAATTAATGCGAACAGACAGACTTGAGGTATGTCGA
GAGTACCAACGTGGCAATTGCAACCGAGGAGAAAATGATTGTCGGTTTGCTCATCCTGCTGACAGCACAATGATTGACACCAATGACAACACAGTCACTG
TGTGTATGGATTACATCAAAGGGAGATGCTCTCGGGAAAAGTGCAAATACTTTCATCCCCCTGCACATTTGCAAGCCAAGATCAAGGCTGCCCAATACCA
GGTCAACCAGGCTGCAGCTGCACAGGCTGCAGCCACCGCAGCTGCCATGGGAATTCCTCAAGCTGTACTTCCCCCATTACCAAAGAGGCCTGCTCTTGAA
AAAACCAACGGTGCCACCGCAGTCTTTAACACTGGTATTTTCCAATACCAACAGGCTCTAGCCAACATGCAGTTACAACAGCATACAGCATTTCTCCCAC
CAGTTCCCATGGTGCACGGTGCTACGCCAGCCACTGTGTCCGCAGCAACAACATCTGCCACAAGTGTTCCCTTCGCTGCAACAGCCACAGCCAACCAGAT
ACCCATAATATCTGCCGAACATCTGACTAGCCACAAGTATGTTACCCAGATGTAGAATTTTCATCACTAAACAATCATGCTAAAGAGGAAAGGACAGTGT
GCTTGGTTAGAGTAAAGGACGAGGTCATTAGCCATATTGTATATATCGTCAAGCAACACACACAAAAGTTCCTCAGCCACAAGACATCCACATATTGCAT
GTTAACCAGAAGAAAAGACAACATTTTCCGGAAATCCACTGCACACTGTTGCCTATACACTTTGTACATTTAATTGATATTTGTGCTGAGGTGATATTCC
TGTCTAAAAGAACAACATTGTCTTTCTTTTCTAGCACAGAGTTATGCATTCAAAGATGCATACCTAGTTAGTTTCCTATATATTCATGCCATCTTGAAAA
GACAGACTATGGTGTAACCATGATTCTATTATGTATTGGTACGTCTGTAGACCAAGATATAATTTTTTAAAAATAAGTTTATTTCTTTCAAGGTTTACAA
ATAACAAAGGTGCACCTTGTATTTAAAATTGCCATTATAGATGAGAGCGTGCATGCACAGTCATTTTTGTTTAAGAGTAATATTTTTAATGTAATAGATT
GTAAGACGTGGTGAGGGAGGGATCTGACAGAGATGAATGTGCCAAGCAAAACCACAACTGTGTATATTTTAAAGCACATCATGGCTTTAAGTACCATGTT
GTTAAGGATTCTCATGAAGTGCCATAGACTGTACATCAAATTAGAGTATTATTTCTTCAGTGTTATTGTTTTCAGAGCCACATTTTGTTGCATATTTGCT
AGTACTAATCAGTCAAAGGGCACCATTCTTTTTTTTTTTTTTTGAAACCAAAGCTGTCTCAGAAATGGCCAATTTAACTTTACAGTAACAATAGACAGCA
CAACACAAACTCTCTCAATACAGATAAACTCACACATACTGGAGATATATATATAATAGATATATATAAAATTATTTTAATGCATTGTAGTGTAATATTT
ATGCATACTATACTGTATAACATGTTATTCAAAAGGGATTGCCATTTCTGAGACACAGTAACAAAAAAATGAGGAAATTATTTTGCTTCTATTTATAGCC
TCTGTCAAAAGTCAAAAGACTATAAATGCTTTGCAAAAATGGTTTCACGTTTGCTTAAATGCTTCATCACAGTCACATTCAAAATAGTGACTCTAAACAA
AGAAGAAAGCAGCACTGTCATCAGATGCATGATAAACCAAAATATGAAAATGGGAAATGTTTAATTAACCTAGTAATTGGGTGGGTTAAGTACATGGGTG
AATTTTATATGTGATTTTTGTTTTGTTTTGTTTTGTTCAGATTAACTGCTTATAGCCTTAGAAAGCCTTTTACAAAATTAAAAAAAAAATAGATGTGCAT
TCAGTTTTTAAGAATGGAATCATCCAAAGGAATTCCTTTTTTTGAGGTTTGGATGTTGCAGCTAGTAAAGGATATTTTTGCTCTGTTCAGCAGTTCTAAA
AATTGCTGAAGTAGGGGCCAGGTCACTGGTAGTTATAGTATGGAATGGGAGAAGTGAAAGTTCAGTTATAGAACTTTCCATACTTCCAAGTTTACTGCAA
GTTTTTATGCTTGAGAGAGATGCTTTCTAATATAAGACTGATGTGTTGATTTTACTGATTGTACTGTACATCTATTAAAGCCTTAGATTATTACATTACG
GGTTGGAACCCATACCAATGTAATTTCAATCGTGTTAAGAAAGTAATGGTGACTTCACATGTTATTGTAGTTAGTTACATTATAGAATATTACTTATTTT
TCTTGTTAAAATGTAGTTTTTCATTTCCTACATTTATTAGATTTTCATTTTCTATTAACAATTGAATACCATTTCAGTTTATAGACTTGTTTTATTAGAT
TTTACCAATGAATTTTTCAAAATACAAAAAAAAGTAGTTTTTCCTTCATAACATACTCAGTTTTGAATTACATGTAGTGTCACATGAATATTCGTATTGT
TAACTAAATGATTTATATTTTACTGATTTAATATTACAGTGTAAGAATGTCAGTCATTGTTAGTTCTTGTCTAGTTTTCATTAAAAGAACAAAGATCTTT
TATATGGATATCTTATAAATATATAATCATTGCTAAGTAAGAAGTTAAGTTGTTGCTATCGCAACAATCCTGGCAGACAATTGAGTAATATTTTGATGAT
TTATTTTGTTTGTAATTAGTTATTATAAGAAGATCTAGATCCTAGATATTAGAATAAAATTTATTTTCTACTGTATCCATTTCAAATGTTAAAATATTGT
TTAATATTTTTGAAATCCCTGAGTATCAGGCCTTGTTATAAATAAGCTGCATAATCAATAAATAGAACAAGGGACTTTTTGTTGATAATCCAAATACTCA
AAGTTTACGTAATGAAAATTATAGCGTGTGTGCAAACTCTTGAGGGTTGATTATGCTGCAATTTAGCATGTTGGAACGTCTAGGGAGAAGGTTGACTTTT
TGCACTTCTGTATATAGTCAAAAGAGAGAAACCTGTATAATAGTAAGATCTTATTTTGAATAAAAACGTCTATAATTACAAGGAGTTTTGTTAAGGCTAA
TACAATGACAGACTGAGCAAAATTGCTTGCAAAAGTGGCACAGAGTTAGCACTCCATACCCCTTCAAACATGTTGCTTTGCTTTCTTGTGGACAGCTTGT
AGTTTGCCAGGATTTTTTCAGCTGGAAAGATACGCCATCCTTTCAAACCCTCATGACTGACAAAAACTCCATGGGGCCAAATCTGCCTGAAGATCATTAC
CAAAAATAGCAGGTACTTCTACCATTAAGGTGAAATCATGGATCAGATATTCCTTACATTTTTCAAAACTACTGCATGTTTAAAACTTCAACAAAAAAAG
AGAGAAAGAACTATACTAAGAACATATATTATTCAGATCAGTTTCTGCCAATTTCAGTGGTTTATTGTTCACAAAAAAATCTTCAAAACAAGTATTGACT
TTCACAAAATTTAAATCATAAACAGGCAAACCAAACAGCACACTGTAGCTATAGTTGTTATGTGATTGTTTTTTAATTGCTGTAGGATCCTGTTCTTTCA
GCAGGTGAAAAATAAAACGCAGTTCAAATTTCATGGTTTTAATTTTCAACTCAGAAGCACTCAAAAATGCAAAATGTGATAATGGGCACTTGTTTAAAAG
AATTAGTGTATCCAGCCTTCACTCCAGCTGGTTAAAAATGTTGCACTTATCAGCAACCCTACCACTTTCATCTGCTGAAAGGACAAATGTGCTTGGTTTT
ACTATTATGTAATCACAACTTACTTTCTGCTTGTAGTTGCTTAAAATTATGTATTTTGTCTTGGGCTGCAATTTGTTTTATGCTTATTTTATTATTACTG
CAGTAGTTGACTTTGCTGTATGGAAAAATAAAGTGAAATTGCCCTAATAAAACTTCTCTTTCTTAAGTA
- refseq_MBNL1.F2 (forward primer)
- refseq_MBNL1.R2 (reverse primer)
- Single exon skipping
Frame 0
LHLIAPSEL*YGAAVYLLWRQYGLLAVEYPGISEVTYYF*RGSFAFSKSHGAKLGIQWPVNYKYYLQ
TRRKADMCLCLCDSFTKEFQNCG*PVT*EKYTV
AVLQMFFPPVSPPGSKTVWCILRRQSHQIAGF*
SNWLLTFNLTLPASLQSPVQGPLSK*THWCA*QS
VEEAGGRPVCTPAARLQC*THEMEDTNGRPRGS
TAPARAPASSCSRRRWATAHDPLLRALSG*QAT
LWPGEAREGAGGKLKSVFLAAACIRSSTSPPWAS
WWGWAGGGGAAQQQRKPDLGDKRLHSDMQQSFH
CS*MSCGAHNAPMTRS*QVPFSVAGILES*LPQ
CLGHLVDSGAASEKKM*EVGTKKCLS*RLCCLEP
LLEQSLLLPCLLPARL*RQHIHPPPLAQTPPFL
LIIKRKALSIWHCPRLL*C*KKSLLKK*DIFCQ
EIKEEKKNHFLDFALNCCICLCQTNQYRLHHQTP
LQIQL*GDSLSDNFAESSLEIRCRRVCHVFMLA
FWAPNWHWEGVTESTRLIPGPTFKRSSTYNPSI
SLKTKTSLN*QFHAVNF*WEIFSLILTTQFSMYF
*SREWGKVF*GDIFIISSAFFFWLLLFFGGVGF
VGFTETFNYL*NLNMAVSVTPIRDTKWLTLEVC
REFQRGTCSRPDTECKFAHPSKSCQVENGRVIAC
FDSLKGRCSRENCKYLHPPPHLKTQLEINGRNN
LIQQKNMAMLAQQMQLANAMMPGAPLQPVPMFS
VAPSLATNASAAAFNPYLGPVSPSLVPAEILPTA
PMLVTGNPGVPVPAAAAAAAQKLMRTDRLEVCR
EYQRGNCNRGENDCRFAHPADSTMIDTNDNTVT
VCMDYIKGRCSREKCKYFHPPAHLQAKIKAAQYQ
VNQAAAAQAAATAAAMGIPQAVLPPLPKRPALE
KTNGATAVFNTGIFQYQQALANMQLQQHTAFLP
PVPMVHGATPATVSAATTSATSVPFAATATANQI
PIISAEHLTSHKYVTQM*NFHH*TIMLKRKGQC
AWLE*RTRSLAILYISSSNTHKSSSATRHPHIA
C*PEEKTTFSGNPLHTVAYTLCTFN*YLC*GDIP
V*KNNIVFLF*HRVMHSKMHT*LVSYIFMPS*K
DRLWCNHDSIMYWYVCRPRYNFLKISLFLSRFT
NNKGAPCI*NCHYR*ERACTVIFV*E*YF*CNRL
*DVVREGSDRDECAKQNHNCVYFKAHHGFKYHV
VKDSHEVP*TVHQIRVLFLQCYCFQSHILLHIC
*Y*SVKGHHSFFFFLKPKLSQKWPI*LYSNNRQH
NTNSLNTDKLTHTGDIYIIDIYKIILMHCSVIF
MHTILYNMLFKRDCHF*DTVTKK*GNYFASIYS
LCQKSKDYKCFAKMVSRLLKCFITVTFKIVTLNK
EESSTVIRCMINQNMKMGNV*LT**LGGLSTWV
NFICDFCFVLFCSD*LLIALESLLQN*KKNRCA
FSF*EWNHPKEFLFLRFGCCS**RIFLLCSAVLK
IAEVGARSLVVIVWNGRSESSVIELSILPSLLQ
VFMLERDAF*YKTDVLILLIVLYIY*SLRLLHY
GLEPIPM*FQSC*ESNGDFTCYCS*LHYRILLIF
LVKM*FFISYIY*IFIFY*QLNTISVYRLVLLD
FTNEFFKIQKKVVFPS*HTQF*ITCSVT*IFVL
LTK*FIFY*FNITV*ECQSLLVLV*FSLKEQRSF
IWISYKYIIIAK*EVKLLLSQQSWQTIE*YFDD
LFCL*LVIIRRSRS*ILE*NLFSTVSISNVKIL
FNIFEIPEYQALL*ISCIINK*NKGLFVDNPNTQ
SLRNENYSVCANS*GLIMLQFSMLERLGRRLTF
CTSVYSQKRETCIIVRSYFE*KRL*LQGVLLRL
IQ*QTEQNCLQKWHRVSTPYPFKHVALLSCGQLV
VCQDFFSWKDTPSFQTLMTDKNSMGPNLPEDHY
QK*QVLLPLR*NHGSDIPYIFQNYCMFKTSTKK
ERKNYTKNIYYSDQFLPISVVYCSQKNLQNKY*L
SQNLNHKQANQTAHCSYSCYVIVF*LL*DPVLS
AGEK*NAVQISWF*FSTQKHSKMQNVIMGTCLK
ELVYPAFTPAG*KCCTYQQPYHFHLLKGQMCLVL
LLCNHNLLSACSCLKLCILSWAAICFMLILLLL
Q*LTLLYGKIK*NCPNKTSLS*V
Frame 1
YIL*HPVNYDMGLRFICSGDNMAYLPWNIPVFQK*HIIFKEGALLSLNHMVQSWEYSGL*ITNIIY
KLEGKLTCVCACVTVSLRNSKTVDSLLLEKNTRW
QSCRCFSRLLALQVQKLFGAF*GGSLTRSQDSS
LIGF*PSI*PCRLPCKARCKGLFQNEPTGVPSS
R*KKREGVRSARPPRGYNAERMRWKIPTGGRGDP
RRPRGLRLPPALGAAGRPPMTRSCGLCPVDRRP
CGPGKRGRAPAES*RAFFSPPRALGARRVRPGL
PGGAGRAGEGPRSSSGSQTSAIRGCTATCNSLFT
AAE*VVAPTMLP*QGADKFHFPSRASWNHDSHN
ALGTWSTVGPPLKKKCERLVLRSAFPDVSAAWN
RF*SSLCFCLACCQLDCDDSTSTLHL*PRHPHFY
L*SREKL*VSGIALGCFSVKRKVC*KSKISSAR
KSRRKKKIIFSILL*TAASVYAKLINTDCTTKL
HCKFSCEEIPFQTTLLKAAWKFGVEGSATFSCLH
FGLQIGTGKGLLRAQG*YQALLLNVHLLTILVF
L*KPKPL*INSFML*ISSGRSFP*Y*RHNFPCT
FKAGSGEKYFEGTFSSSVQLFFFGCCSFLGGLGL
LVSLKHLTTCKI*TWLLVSHQFGTQNG*HWKYV
ESSRGGLAHGQTRNVNLHILRKAAKLKMDE*SP
ALIH*KAVAPGRTANIFIHPHI*KRSWR*MDAIT
*FSRRTWPCWPSKCN*PMP*CLVPHYNPCQCFQ
LHQA*PPMHQQPPLIPIWDLFLQAWSRQRSCRL
HQCWLQGIRVSLYLQLLQLLHRN*CEQTDLRYVE
STNVAIATEEKMIVGLLILLTAQ*LTPMTTQSL
CVWITSKGDALGKSANTFIPLHICKPRSRLPNT
RSTRLQLHRLQPPQLPWEFLKLYFPHYQRGLLLK
KPTVPPQSLTLVFSNTNRL*PTCSYNSIQHFSH
QFPWCTVLRQPLCPQQQHLPQVFPSLQQPQPTR
YP*YLPNI*LATSMLPRCRIFITKQSC*RGKDSV
LG*SKGRGH*PYCIYRQATHTKVPQPQDIHILH
VNQKKRQHFPEIHCTLLPIHFVHLIDICAEVIF
LSKRTTLSFFSSTELCIQRCIPS*FPIYSCHLEK
TDYGVTMILLCIGTSVDQDIIF*K*VYFFQGLQ
ITKVHLVFKIAIIDESVHAQSFLFKSNIFNVID
CKTW*GRDLTEMNVPSKTTTVYILKHIMALSTML
LRILMKCHRLYIKLEYYFFSVIVFRATFCCIFA
STNQSKGTILFFFF*NQSCLRNGQFNFTVTIDS
TTQTLSIQINSHILEIYI**IYIKLF*CIVV*YL
CILYCITCYSKGIAISETQ*QKNEEIILLLFIA
SVKSQKTINALQKWFHVCLNASSQSHSK**L*T
KKKAALSSDA**TKI*KWEMFN*PSNWVG*VHG*
ILYVIFVLFCFVQINCL*P*KAFYKIKKKIDVH
SVFKNGIIQRNSFF*GLDVAASKGYFCSVQQF*
KLLK*GPGHW*L*YGMGEVKVQL*NFPYFQVYCK
FLCLREMLSNIRLMC*FY*LYCTSIKALDYYIT
GWNPYQCNFNRVKKVMVTSHVIVVSYIIEYYLF
FLLKCSFSFPTFIRFSFSINN*IPFQFIDLFY*I
LPMNFSKYKKK*FFLHNILSFELHVVSHEYSYC
*LNDLYFTDLILQCKNVSHC*FLSSFH*KNKDL
LYGYLINI*SLLSKKLSCCYRNNPGRQLSNILMI
YFVCN*LL*EDLDPRY*NKIYFLLYPFQMLKYC
LIFLKSLSIRPCYK*AA*SINRTRDFLLIIQIL
KVYVMKIIACVQTLEG*LCCNLACWNV*GEG*LF
ALLYIVKREKPV***DLILNKNVYNYKEFC*G*
YNDRLSKIACKSGTELALHTPSNMLLCFLVDSL
*FARIFSAGKIRHPFKPS*LTKTPWGQICLKIIT
KNSRYFYH*GEIMDQIFLTFFKTTACLKLQQKK
RERTILRTYIIQISFCQFQWFIVHKKIFKTSID
FHKI*IINRQTKQHTVAIVVM*LFFNCCRILFFQ
QVKNKTQFKFHGFNFQLRSTQKCKM**WALV*K
N*CIQPSLQLVKNVALISNPTTFIC*KDKCAWF
YYYVITTYFLLVVA*NYVFCLGLQFVLCLFYYYC
SS*LCCMEK*SEIALIKLLFLK
Frame 2
TSYSTQ*IMIWGCGLFALATIWLTCRGISRYFRSNILFLKRELCFL*ITWCKVGNTVACKLQILFT
N*KES*HVFVPV*QFH*GIPKLWIACYLRKIHG
GSLADVFPAC*PSRFKNCLVHFKAAVSPDRRILV
*LASNLQSNLAGFLAKPGARASFKMNPLVCLAV
GRRSGRASGLHARREVTMLNA*DGRYQREAEGI
HGARAGSGFLLLSAPLGDRP*PALAGSVRLTGDP
VARGSAGGRRRKVEERFSRRRVH*ELDESALGF
LVGLGGRGRGRAAAAEARPRR*EAAQRHATVFS
LQLNELWRPQCSHDKELTSSIFRRGHLGIMTPTM
PWALGRQWGRL*KKNVRGWY*EVPFLTSLLLGT
ASRAVSAFALLAAS*TVTTAHPPSTSSPDTPIS
TYNQEKSSKYLALP*AALVLKEKFAEKVRYLLPG
NQGGKKKSFSRFCSKLLHLSMPN*SIPIAPPNS
IANSAVRRFPFRQLC*KQLGNSVSKGLPRFHAC
ILGSKLALGRGY*EHKADTRPYF*TFIYLQS*YF
SKNQNLFELTVSCCEFLVGDLFLDIDDTIFHVL
LKQGVGKSILRGHFHHQFSFFFLVVALFWGGWV
CWFH*NI*LPVKSKHGC*CHTNSGHKMANTGSM*
RVPEGDLLTARHGM*ICTSFEKLPS*KWTSNRL
L*FIERPLLQGELQISSSTPTFKNAVGDKWTQ*
LDSAEEHGHVGPANATSQCHDAWCPITTRANVFS
CTKLSHQCISSRL*SLSGTCFSKPGPGRDLADC
TNVGYRESGCPCTCSCCSCCTEINANRQT*GMS
RVPTWQLQPRRK*LSVCSSC*QHND*HQ*QHSHC
VYGLHQREMLSGKVQILSSPCTFASQDQGCPIP
GQPGCSCTGCSHRSCHGNSSSCTSPITKEACS*
KNQRCHRSL*HWYFPIPTGSSQHAVTTAYSISPT
SSHGARCYASHCVRSNNICHKCSLRCNSHSQPD
THNICRTSD*PQVCYPDVEFSSLNNHAKEERTV
CLVRVKDEVISHIVYIVKQHTQKFLSHKTSTYCM
LTRRKDNIFRKSTAHCCLYTLYI*LIFVLR*YS
CLKEQHCLSFLAQSYAFKDAYLVSFLYIHAILK
RQTMV*P*FYYVLVRL*TKI*FFKNKFISFKVYK
*QRCTLYLKLPL*MRACMHSHFCLRVIFLM**I
VRRGEGGI*QR*MCQAKPQLCIF*STSWL*VPC
C*GFS*SAIDCTSN*SIISSVLLFSEPHFVAYLL
VLISQRAPFFFFFFETKAVSEMANLTLQ*Q*TA
QHKLSQYR*THTYWRYIYNRYI*NYFNAL*CNI
YAYYTV*HVIQKGLPFLRHSNKKMRKLFCFYL*P
LSKVKRL*MLCKNGFTFA*MLHHSHIQNSDSKQ
RRKQHCHQMHDKPKYENGKCLINLVIGWVKYMG
EFYM*FLFCFVLFRLTAYSLRKPFTKLKKK*MCI
QFLRMESSKGIPFFEVWMLQLVKDIFALFSSSK
NC*SRGQVTGSYSMEWEK*KFSYRTFHTSKFTA
SFYA*ERCFLI*D*CVDFTDCTVHLLKP*IITLR
VGTHTNVISIVLRK*W*LHMLL*LVTL*NITYF
SC*NVVFHFLHLLDFHFLLTIEYHFSL*TCFIR
FYQ*IFQNTKKSSFSFITYSVLNYM*CHMNIRIV
N*MIYILLI*YYSVRMSVIVSSCLVFIKRTKIF
YMDIL*IYNHC*VRS*VVAIATILADN*VIF**
FILFVISYYKKI*ILDIRIKFIFYCIHFKC*NIV
*YF*NP*VSGLVINKLHNQ*IEQGTFC**SKYS
KFT**KL*RVCKLLRVDYAAI*HVGTSREKVDF
LHFCI*SKERNLYNSKILF*IKTSIITRSFVKAN
TMTD*AKLLAKVAQS*HSIPLQTCCFAFLWTAC
SLPGFFQLERYAILSNPHD*QKLHGAKSA*RSL
PKIAGTSTIKVKSWIRYSLHFSKLLHV*NFNKKR
EKELY*EHILFRSVSANFSGLLFTKKSSKQVLT
FTKFKS*TGKPNSTL*L*LLCDCFLIAVGSCSF
SR*KIKRSSNFMVLIFNSEALKNAKCDNGHLFKR
ISVSSLHSSWLKMLHLSATLPLSSAERTNVLGF
TIM*SQLTFCL*LLKIMYFVLGCNLFYAYFIIT
AVVDFAVWKNKVKLP**NFSFLS
Sequence NM_207294
GTTGGTACTAAGAAGTGCCTTTCCTGACGTCTCTGCTGCTTGGAACCGCTTCTAGAGCAGTCTCTGCTTTTGCCTTGCTTGCTGCCAGCTAGACTGTGACGACAGCACATCCACCCTCCACCTCTAGCCCAGACACCCCCATTTCTACTTATAATCAAGAGAAAAGCTCTAAGTATCTGGCATTGCCCTAGGCTGCTTTA
GTGTTAAAAGAAAAGTTTGCTGAAAAAGTAAGATATCTTCTGCCAGGAAATCAAGGAGGAAAAAAAAAATCATTTTCTCGATTTTGCTCTAAACTGCTGC
ATCTGTCTATGCCAAACTAATCAATACCGATTGCACCACCAAACTCCATTGCAAATTCAGCTGTGAGGAGATTCCCTTTCAGACAACTTTGCTGAAAGCA
GCTTGGAAATTCGGTGTCGAAGGGTCTGCCACGTTTTCATGCTTGCATTTTGGGCTCCAAATTGGCACTGGGAAGGGGTTACTGAGAGCACAAGGCTGAT
ACCAGGCCCTACTTTTAAACGTTCATCTACTTACAATCCTAGTATTTCTCTAAAAACCAAAACCTCTTTGAATTAACAGTTTCATGCTGTGAATTTCTAG
TGGGAGATCTTTTCCTTGATATTGACGACACAATTTTCCATGTACTTTTAAAGCAGGGAGTGGGGAAAAGTATTTTGAGGGGACATTTTCATCATCAGTT
CAGCTTTTTTTTTTTGGTTGTTGCTCTTTTTTGGGGGGGTTGGGTTTGTTGGTTTCACTGAAACATTTAACTACCTGTAAAATCTAAACATGGCTGTTAG
TGTCACACCAATTCGGGACACAAAATGGCTAACACTGGAAGTATGTAGAGAGTTCCAGAGGGGGACTTGCTCACGGCCAGACACGGAATGTAAATTTGCA
CATCCTTCGAAAAGCTGCCAAGTTGAAAATGGACGAGTAATCGCCTGCTTTGATTCATTGAAAGGCCGTTGCTCCAGGGAGAACTGCAAATATCTTCATC
CACCCCCACATTTAAAAACGCAGTTGGAGATAAATGGACGCAATAACTTGATTCAGCAGAAGAACATGGCCATGTTGGCCCAGCAAATGCAACTAGCCAA
TGCCATGATGCCTGGTGCCCCATTACAACCCGTGGTATGTCGAGAGTACCAACGTGGCAATTGCAACCGAGGAGAAAATGATTGTCGGTTTGCTCATCCT
GCTGACAGCACAATGATTGACACCAATGACAACACAGTCACTGTGTGTATGGATTACATCAAAGGGAGATGCTCTCGGGAAAAGTGCAAATACTTTCATC
CCCCTGCACATTTGCAAGCCAAGATCAAGGCTGCCCAATACCAGGTCAACCAGGCTGCAGCTGCACAGGCTGCAGCCACCGCAGCTGCCATGGGAATTCC
TCAAGCTGTACTTCCCCCATTACCAAAGAGGCCTGCTCTTGAAAAAACCAACGGTGCCACCGCAGTCTTTAACACTGGTATTTTCCAATACCAACAGGCT
CTAGCCAACATGCAGTTACAACAGCATACAGCATTTCTCCCACCAGTTCCCATGGTGCACGGTGCTACGCCAGCCACTGTGTCCGCAGCAACAACATCTG
CCACAAGTGTTCCCTTCGCTGCAACAGCCACAGCCAACCAGATACCCATAATATCTGCCGAACATCTGACTAGCCACAAGTATGTTACCCAGATGTAGAA
TTTTCATCACTAAACAATCATGCTAAAGAGGAAAGGACAGTGTGCTTGGTTAGAGTAAAGGACGAGGTCATTAGCCATATTGTATATATCGTCAAGCAAC
ACACACAAAAGTTCCTCAGCCACAAGACATCCACATATTGCATGTTAACCAGAAGAAAAGACAACATTTTCCGGAAATCCACTGCACACTGTTGCCTATA
CACTTTGTACATTTAATTGATATTTGTGCTGAGGTGATATTCCTGTCTAAAAGAACAACATTGTCTTTCTTTTCTAGCACAGAGTTATGCATTCAAAGAT
GCATACCTAGTTAGTTTCCTATATATTCATGCCATCTTGAAAAGACAGACTATGGTGTAACCATGATTCTATTATGTATTGGTACGTCTGTAGACCAAGA
TATAATTTTTTAAAAATAAGTTTATTTCTTTCAAGGTTTACAAATAACAAAGGTGCACCTTGTATTTAAAATTGCCATTATAGATGAGAGCGTGCATGCA
CAGTCATTTTTGTTTAAGAGTAATATTTTTAATGTAATAGATTGTAAGACGTGGTGAGGGAGGGATCTGACAGAGATGAATGTGCCAAGCAAAACCACAA
CTGTGTATATTTTAAAGCACATCATGGCTTTAAGTACCATGTTGTTAAGGATTCTCATGAAGTGCCATAGACTGTACATCAAATTAGAGTATTATTTCTT
CAGTGTTATTGTTTTCAGAGCCACATTTTGTTGCATATTTGCTAGTACTAATCAGTCAAAGGGCACCATTCTTTTTTTTTTTTTTTGAAACCAAAGCTGT
CTCAGAAATGGCCAATTTAACTTTACAGTAACAATAGACAGCACAACACAAACTCTCTCAATACAGATAAACTCACACATACTGGAGATATATATATAAT
AGATATATATAAAATTATTTTAATGCATTGTAGTGTAATATTTATGCATACTATACTGTATAACATGTTATTCAAAAGGGATTGCCATTTCTGAGACACA
GTAACAAAAAAATGAGGAAATTATTTTGCTTCTATTTATAGCCTCTGTCAAAAGTCAAAAGACTATAAATGCTTTGCAAAAATGGTTTCACGTTTGCTTA
AATGCTTCATCACAGTCACATTCAAAATAGTGACTCTAAACAAAGAAGAAAGCAGCACTGTCATCAGATGCATGATAAACCAAAATATGAAAATGGGAAA
TGTTTAATTAACCTAGTAATTGGGTGGGTTAAGTACATGGGTGAATTTTATATGTGATTTTTGTTTTGTTTTGTTTTGTTCAGATTAACTGCTTATAGCC
TTAGAAAGCCTTTTACAAAATTAAAAAAAAAATAGATGTGCATTCAGTTTTTAAGAATGGAATCATCCAAAGGAATTCCTTTTTTTGAGGTTTGGATGTT
GCAGCTAGTAAAGGATATTTTTGCTCTGTTCAGCAGTTCTAAAAATTGCTGAAGTAGGGGCCAGGTCACTGGTAGTTATAGTATGGAATGGGAGAAGTGA
AAGTTCAGTTATAGAACTTTCCATACTTCCAAGTTTACTGCAAGTTTTTATGCTTGAGAGAGATGCTTTCTAATATAAGACTGATGTGTTGATTTTACTG
ATTGTACTGTACATCTATTAAAGCCTTAGATTATTACATTACGGGTTGGAACCCATACCAATGTAATTTCAATCGTGTTAAGAAAGTAATGGTGACTTCA
CATGTTATTGTAGTTAGTTACATTATAGAATATTACTTATTTTTCTTGTTAAAATGTAGTTTTTCATTTCCTACATTTATTAGATTTTCATTTTCTATTA
ACAATTGAATACCATTTCAGTTTATAGACTTGTTTTATTAGATTTTACCAATGAATTTTTCAAAATACAAAAAAAAGTAGTTTTTCCTTCATAACATACT
CAGTTTTGAATTACATGTAGTGTCACATGAATATTCGTATTGTTAACTAAATGATTTATATTTTACTGATTTAATATTACAGTGTAAGAATGTCAGTCAT
TGTTAGTTCTTGTCTAGTTTTCATTAAAAGAACAAAGATCTTTTATATGGATATCTTATAAATATATAATCATTGCTAAGTAAGAAGTTAAGTTGTTGCT
ATCGCAACAATCCTGGCAGACAATTGAGTAATATTTTGATGATTTATTTTGTTTGTAATTAGTTATTATAAGAAGATCTAGATCCTAGATATTAGAATAA
AATTTATTTTCTACTGTATCCATTTCAAATGTTAAAATATTGTTTAATATTTTTGAAATCCCTGAGTATCAGGCCTTGTTATAAATAAGCTGCATAATCA
ATAAATAGAACAAGGGACTTTTTGTTGATAATCCAAATACTCAAAGTTTACGTAATGAAAATTATAGCGTGTGTGCAAACTCTTGAGGGTTGATTATGCT
GCAATTTAGCATGTTGGAACGTCTAGGGAGAAGGTTGACTTTTTGCACTTCTGTATATAGTCAAAAGAGAGAAACCTGTATAATAGTAAGATCTTATTTT
GAATAAAAACGTCTATAATTACAAGGAGTTTTGTTAAGGCTAATACAATGACAGACTGAGCAAAATTGCTTGCAAAAGTGGCACAGAGTTAGCACTCCAT
ACCCCTTCAAACATGTTGCTTTGCTTTCTTGTGGACAGCTTGTAGTTTGCCAGGATTTTTTCAGCTGGAAAGATACGCCATCCTTTCAAACCCTCATGAC
TGACAAAAACTCCATGGGGCCAAATCTGCCTGAAGATCATTACCAAAAATAGCAGGTACTTCTACCATTAAGGTGAAATCATGGATCAGATATTCCTTAC
ATTTTTCAAAACTACTGCATGTTTAAAACTTCAACAAAAAAAGAGAGAAAGAACTATACTAAGAACATATATTATTCAGATCAGTTTCTGCCAATTTCAG
TGGTTTATTGTTCACAAAAAAATCTTCAAAACAAGTATTGACTTTCACAAAATTTAAATCATAAACAGGCAAACCAAACAGCACACTGTAGCTATAGTTG
TTATGTGATTGTTTTTTAATTGCTGTAGGATCCTGTTCTTTCAGCAGGTGAAAAATAAAACGCAGTTCAAATTTCATGGTTTTAATTTTCAACTCAGAAG
CACTCAAAAATGCAAAATGTGATAATGGGCACTTGTTTAAAAGAATTAGTGTATCCAGCCTTCACTCCAGCTGGTTAAAAATGTTGCACTTATCAGCAAC
CCTACCACTTTCATCTGCTGAAAGGACAAATGTGCTTGGTTTTACTATTATGTAATCACAACTTACTTTCTGCTTGTAGTTGCTTAAAATTATGTATTTT
GTCTTGGGCTGCAATTTGTTTTATGCTTATTTTATTATTACTGCAGTAGTTGACTTTGCTGTATGGAAAAATAAAGTGAAATTGCCCTAATAAAACTTCT
CTTTCTTAAGTA
- refseq_MBNL1.F2 (forward primer)
- refseq_MBNL1.R2 (reverse primer)
- Single exon skipping
Frame 0
VGTKKCLS*RLCCLEPLLEQSLLLPCLLPARL*RQHIHPPPLAQTPPFLLIIKRKALSIWHCPRLL*
C*KKSLLKK*DIFCQEIKEEKKNHFLDFALNCC
ICLCQTNQYRLHHQTPLQIQL*GDSLSDNFAES
SLEIRCRRVCHVFMLAFWAPNWHWEGVTESTRLI
PGPTFKRSSTYNPSISLKTKTSLN*QFHAVNF*
WEIFSLILTTQFSMYF*SREWGKVF*GDIFIIS
SAFFFWLLLFFGGVGFVGFTETFNYL*NLNMAVS
VTPIRDTKWLTLEVCREFQRGTCSRPDTECKFA
HPSKSCQVENGRVIACFDSLKGRCSRENCKYLH
PPPHLKTQLEINGRNNLIQQKNMAMLAQQMQLAN
AMMPGAPLQPVVCREYQRGNCNRGENDCRFAHP
ADSTMIDTNDNTVTVCMDYIKGRCSREKCKYFH
PPAHLQAKIKAAQYQVNQAAAAQAAATAAAMGIP
QAVLPPLPKRPALEKTNGATAVFNTGIFQYQQA
LANMQLQQHTAFLPPVPMVHGATPATVSAATTS
ATSVPFAATATANQIPIISAEHLTSHKYVTQM*N
FHH*TIMLKRKGQCAWLE*RTRSLAILYISSSN
THKSSSATRHPHIAC*PEEKTTFSGNPLHTVAY
TLCTFN*YLC*GDIPV*KNNIVFLF*HRVMHSKM
HT*LVSYIFMPS*KDRLWCNHDSIMYWYVCRPR
YNFLKISLFLSRFTNNKGAPCI*NCHYR*ERAC
TVIFV*E*YF*CNRL*DVVREGSDRDECAKQNHN
CVYFKAHHGFKYHVVKDSHEVP*TVHQIRVLFL
QCYCFQSHILLHIC*Y*SVKGHHSFFFFLKPKL
SQKWPI*LYSNNRQHNTNSLNTDKLTHTGDIYII
DIYKIILMHCSVIFMHTILYNMLFKRDCHF*DT
VTKK*GNYFASIYSLCQKSKDYKCFAKMVSRLL
KCFITVTFKIVTLNKEESSTVIRCMINQNMKMGN
V*LT**LGGLSTWVNFICDFCFVLFCSD*LLIA
LESLLQN*KKNRCAFSF*EWNHPKEFLFLRFGC
CS**RIFLLCSAVLKIAEVGARSLVVIVWNGRSE
SSVIELSILPSLLQVFMLERDAF*YKTDVLILL
IVLYIY*SLRLLHYGLEPIPM*FQSC*ESNGDF
TCYCS*LHYRILLIFLVKM*FFISYIY*IFIFY*
QLNTISVYRLVLLDFTNEFFKIQKKVVFPS*HT
QF*ITCSVT*IFVLLTK*FIFY*FNITV*ECQS
LLVLV*FSLKEQRSFIWISYKYIIIAK*EVKLLL
SQQSWQTIE*YFDDLFCL*LVIIRRSRS*ILE*
NLFSTVSISNVKILFNIFEIPEYQALL*ISCII
NK*NKGLFVDNPNTQSLRNENYSVCANS*GLIML
QFSMLERLGRRLTFCTSVYSQKRETCIIVRSYF
E*KRL*LQGVLLRLIQ*QTEQNCLQKWHRVSTP
YPFKHVALLSCGQLVVCQDFFSWKDTPSFQTLMT
DKNSMGPNLPEDHYQK*QVLLPLR*NHGSDIPY
IFQNYCMFKTSTKKERKNYTKNIYYSDQFLPIS
VVYCSQKNLQNKY*LSQNLNHKQANQTAHCSYSC
YVIVF*LL*DPVLSAGEK*NAVQISWF*FSTQK
HSKMQNVIMGTCLKELVYPAFTPAG*KCCTYQQ
PYHFHLLKGQMCLVLLLCNHNLLSACSCLKLCIL
SWAAICFMLILLLLQ*LTLLYGKIK*NCPNKTS
LS*V
Frame 1
LVLRSAFPDVSAAWNRF*SSLCFCLACCQLDCDDSTSTLHL*PRHPHFYL*SREKL*VSGIALGCF
SVKRKVC*KSKISSARKSRRKKKIIFSILL*TAA
SVYAKLINTDCTTKLHCKFSCEEIPFQTTLLKA
AWKFGVEGSATFSCLHFGLQIGTGKGLLRAQG*
YQALLLNVHLLTILVFL*KPKPL*INSFML*ISS
GRSFP*Y*RHNFPCTFKAGSGEKYFEGTFSSSV
QLFFFGCCSFLGGLGLLVSLKHLTTCKI*TWLL
VSHQFGTQNG*HWKYVESSRGGLAHGQTRNVNLH
ILRKAAKLKMDE*SPALIH*KAVAPGRTANIFI
HPHI*KRSWR*MDAIT*FSRRTWPCWPSKCN*P
MP*CLVPHYNPWYVESTNVAIATEEKMIVGLLIL
LTAQ*LTPMTTQSLCVWITSKGDALGKSANTFI
PLHICKPRSRLPNTRSTRLQLHRLQPPQLPWEF
LKLYFPHYQRGLLLKKPTVPPQSLTLVFSNTNRL
*PTCSYNSIQHFSHQFPWCTVLRQPLCPQQQHL
PQVFPSLQQPQPTRYP*YLPNI*LATSMLPRCR
IFITKQSC*RGKDSVLG*SKGRGH*PYCIYRQAT
HTKVPQPQDIHILHVNQKKRQHFPEIHCTLLPI
HFVHLIDICAEVIFLSKRTTLSFFSSTELCIQR
CIPS*FPIYSCHLEKTDYGVTMILLCIGTSVDQD
IIF*K*VYFFQGLQITKVHLVFKIAIIDESVHA
QSFLFKSNIFNVIDCKTW*GRDLTEMNVPSKTT
TVYILKHIMALSTMLLRILMKCHRLYIKLEYYFF
SVIVFRATFCCIFASTNQSKGTILFFFF*NQSC
LRNGQFNFTVTIDSTTQTLSIQINSHILEIYI*
*IYIKLF*CIVV*YLCILYCITCYSKGIAISETQ
*QKNEEIILLLFIASVKSQKTINALQKWFHVCL
NASSQSHSK**L*TKKKAALSSDA**TKI*KWE
MFN*PSNWVG*VHG*ILYVIFVLFCFVQINCL*P
*KAFYKIKKKIDVHSVFKNGIIQRNSFF*GLDV
AASKGYFCSVQQF*KLLK*GPGHW*L*YGMGEV
KVQL*NFPYFQVYCKFLCLREMLSNIRLMC*FY*
LYCTSIKALDYYITGWNPYQCNFNRVKKVMVTS
HVIVVSYIIEYYLFFLLKCSFSFPTFIRFSFSI
NN*IPFQFIDLFY*ILPMNFSKYKKK*FFLHNIL
SFELHVVSHEYSYC*LNDLYFTDLILQCKNVSH
C*FLSSFH*KNKDLLYGYLINI*SLLSKKLSCC
YRNNPGRQLSNILMIYFVCN*LL*EDLDPRY*NK
IYFLLYPFQMLKYCLIFLKSLSIRPCYK*AA*S
INRTRDFLLIIQILKVYVMKIIACVQTLEG*LC
CNLACWNV*GEG*LFALLYIVKREKPV***DLIL
NKNVYNYKEFC*G*YNDRLSKIACKSGTELALH
TPSNMLLCFLVDSL*FARIFSAGKIRHPFKPS*
LTKTPWGQICLKIITKNSRYFYH*GEIMDQIFLT
FFKTTACLKLQQKKRERTILRTYIIQISFCQFQ
WFIVHKKIFKTSIDFHKI*IINRQTKQHTVAIV
VM*LFFNCCRILFFQQVKNKTQFKFHGFNFQLRS
TQKCKM**WALV*KN*CIQPSLQLVKNVALISN
PTTFIC*KDKCAWFYYYVITTYFLLVVA*NYVF
CLGLQFVLCLFYYYCSS*LCCMEK*SEIALIKLL
FLK
Frame 2
WY*EVPFLTSLLLGTASRAVSAFALLAAS*TVTTAHPPSTSSPDTPISTYNQEKSSKYLALP*AAL
VLKEKFAEKVRYLLPGNQGGKKKSFSRFCSKLL
HLSMPN*SIPIAPPNSIANSAVRRFPFRQLC*KQ
LGNSVSKGLPRFHACILGSKLALGRGY*EHKAD
TRPYF*TFIYLQS*YFSKNQNLFELTVSCCEFL
VGDLFLDIDDTIFHVLLKQGVGKSILRGHFHHQF
SFFFLVVALFWGGWVCWFH*NI*LPVKSKHGC*
CHTNSGHKMANTGSM*RVPEGDLLTARHGM*IC
TSFEKLPS*KWTSNRLL*FIERPLLQGELQISSS
TPTFKNAVGDKWTQ*LDSAEEHGHVGPANATSQ
CHDAWCPITTRGMSRVPTWQLQPRRK*LSVCSS
C*QHND*HQ*QHSHCVYGLHQREMLSGKVQILSS
PCTFASQDQGCPIPGQPGCSCTGCSHRSCHGNS
SSCTSPITKEACS*KNQRCHRSL*HWYFPIPTG
SSQHAVTTAYSISPTSSHGARCYASHCVRSNNIC
HKCSLRCNSHSQPDTHNICRTSD*PQVCYPDVE
FSSLNNHAKEERTVCLVRVKDEVISHIVYIVKQ
HTQKFLSHKTSTYCMLTRRKDNIFRKSTAHCCLY
TLYI*LIFVLR*YSCLKEQHCLSFLAQSYAFKD
AYLVSFLYIHAILKRQTMV*P*FYYVLVRL*TK
I*FFKNKFISFKVYK*QRCTLYLKLPL*MRACMH
SHFCLRVIFLM**IVRRGEGGI*QR*MCQAKPQ
LCIF*STSWL*VPCC*GFS*SAIDCTSN*SIIS
SVLLFSEPHFVAYLLVLISQRAPFFFFFFETKAV
SEMANLTLQ*Q*TAQHKLSQYR*THTYWRYIYN
RYI*NYFNAL*CNIYAYYTV*HVIQKGLPFLRH
SNKKMRKLFCFYL*PLSKVKRL*MLCKNGFTFA*
MLHHSHIQNSDSKQRRKQHCHQMHDKPKYENGK
CLINLVIGWVKYMGEFYM*FLFCFVLFRLTAYS
LRKPFTKLKKK*MCIQFLRMESSKGIPFFEVWML
QLVKDIFALFSSSKNC*SRGQVTGSYSMEWEK*
KFSYRTFHTSKFTASFYA*ERCFLI*D*CVDFT
DCTVHLLKP*IITLRVGTHTNVISIVLRK*W*LH
MLL*LVTL*NITYFSC*NVVFHFLHLLDFHFLL
TIEYHFSL*TCFIRFYQ*IFQNTKKSSFSFITY
SVLNYM*CHMNIRIVN*MIYILLI*YYSVRMSVI
VSSCLVFIKRTKIFYMDIL*IYNHC*VRS*VVA
IATILADN*VIF**FILFVISYYKKI*ILDIRI
KFIFYCIHFKC*NIV*YF*NP*VSGLVINKLHNQ
*IEQGTFC**SKYSKFT**KL*RVCKLLRVDYA
AI*HVGTSREKVDFLHFCI*SKERNLYNSKILF
*IKTSIITRSFVKANTMTD*AKLLAKVAQS*HSI
PLQTCCFAFLWTACSLPGFFQLERYAILSNPHD
*QKLHGAKSA*RSLPKIAGTSTIKVKSWIRYSL
HFSKLLHV*NFNKKREKELY*EHILFRSVSANFS
GLLFTKKSSKQVLTFTKFKS*TGKPNSTL*L*L
LCDCFLIAVGSCSFSR*KIKRSSNFMVLIFNSE
ALKNAKCDNGHLFKRISVSSLHSSWLKMLHLSAT
LPLSSAERTNVLGFTIM*SQLTFCL*LLKIMYF
VLGCNLFYAYFIITAVVDFAVWKNKVKLP**NF
SFLS
Sequence NM_207295
GTTGGTACTAAGAAGTGCCTTTCCTGACGTCTCTGCTGCTTGGAACCGCTTCTAGAGCAGTCTCTGCTTTTGCCTTGCTTGCTGCCAGCTAGACTGTGACGACAGCACATCCACCCTCCACCTCTAGCCCAGACACCCCCATTTCTACTTATAATCAAGAGAAAAGCTCTAAGTATCTGGCATTGCCCTAGGCTGCTTTA
GTGTTAAAAGAAAAGTTTGCTGAAAAAGTAAGATATCTTCTGCCAGGAAATCAAGGAGGAAAAAAAAAATCATTTTCTCGATTTTGCTCTAAACTGCTGC
ATCTGTCTATGCCAAACTAATCAATACCGATTGCACCACCAAACTCCATTGCAAATTCAGCTGTGAGGAGATTCCCTTTCAGACAACTTTGCTGAAAGCA
GCTTGGAAATTCGGTGTCGAAGGGTCTGCCACGTTTTCATGCTTGCATTTTGGGCTCCAAATTGGCACTGGGAAGGGGTTACTGAGAGCACAAGGCTGAT
ACCAGGCCCTACTTTTAAACGTTCATCTACTTACAATCCTAGTATTTCTCTAAAAACCAAAACCTCTTTGAATTAACAGTTTCATGCTGTGAATTTCTAG
TGGGAGATCTTTTCCTTGATATTGACGACACAATTTTCCATGTACTTTTAAAGCAGGGAGTGGGGAAAAGTATTTTGAGGGGACATTTTCATCATCAGTT
CAGCTTTTTTTTTTTGGTTGTTGCTCTTTTTTGGGGGGGTTGGGTTTGTTGGTTTCACTGAAACATTTAACTACCTGTAAAATCTAAACATGGCTGTTAG
TGTCACACCAATTCGGGACACAAAATGGCTAACACTGGAAGTATGTAGAGAGTTCCAGAGGGGGACTTGCTCACGGCCAGACACGGAATGTAAATTTGCA
CATCCTTCGAAAAGCTGCCAAGTTGAAAATGGACGAGTAATCGCCTGCTTTGATTCATTGAAAGGCCGTTGCTCCAGGGAGAACTGCAAATATCTTCATC
CACCCCCACATTTAAAAACGCAGTTGGAGATAAATGGACGCAATAACTTGATTCAGCAGAAGAACATGGCCATGTTGGCCCAGCAAATGCAACTAGCCAA
TGCCATGATGCCTGGTGCCCCATTACAACCCGTGGTATGTCGAGAGTACCAACGTGGCAATTGCAACCGAGGAGAAAATGATTGTCGGTTTGCTCATCCT
GCTGACAGCACAATGATTGACACCAATGACAACACAGTCACTGTGTGTATGGATTACATCAAAGGGAGATGCTCTCGGGAAAAGTGCAAATACTTTCATC
CCCCTGCACATTTGCAAGCCAAGATCAAGGCTGCCCAATACCAGGTCAACCAGGCTGCAGCTGCACAGGCTGCAGCCACCGCAGCTGCCATGGGAATTCC
TCAAGCTGTACTTCCCCCATTACCAAAGAGGCCTGCTCTTGAAAAAACCAACGGTGCCACCGCAGTCTTTAACACTGGTATTTTCCAATACCAACAGGCT
CTAGCCAACATGCAGTTACAACAGCATACAGCATTTCTCCCACCAGGCTCAATATTGTGCATGACACCCGCTACAAGTGTTGTTCCCATGGTGCACGGTG
CTACGCCAGCCACTGTGTCCGCAGCAACAACATCTGCCACAAGTGTTCCCTTCGCTGCAACAGCCACAGCCAACCAGATACCCATAATATCTGCCGAACA
TCTGACTAGCCACAAGTATGTTACCCAGATGTAGAATTTTCATCACTAAACAATCATGCTAAAGAGGAAAGGACAGTGTGCTTGGTTAGAGTAAAGGACG
AGGTCATTAGCCATATTGTATATATCGTCAAGCAACACACACAAAAGTTCCTCAGCCACAAGACATCCACATATTGCATGTTAACCAGAAGAAAAGACAA
CATTTTCCGGAAATCCACTGCACACTGTTGCCTATACACTTTGTACATTTAATTGATATTTGTGCTGAGGTGATATTCCTGTCTAAAAGAACAACATTGT
CTTTCTTTTCTAGCACAGAGTTATGCATTCAAAGATGCATACCTAGTTAGTTTCCTATATATTCATGCCATCTTGAAAAGACAGACTATGGTGTAACCAT
GATTCTATTATGTATTGGTACGTCTGTAGACCAAGATATAATTTTTTAAAAATAAGTTTATTTCTTTCAAGGTTTACAAATAACAAAGGTGCACCTTGTA
TTTAAAATTGCCATTATAGATGAGAGCGTGCATGCACAGTCATTTTTGTTTAAGAGTAATATTTTTAATGTAATAGATTGTAAGACGTGGTGAGGGAGGG
ATCTGACAGAGATGAATGTGCCAAGCAAAACCACAACTGTGTATATTTTAAAGCACATCATGGCTTTAAGTACCATGTTGTTAAGGATTCTCATGAAGTG
CCATAGACTGTACATCAAATTAGAGTATTATTTCTTCAGTGTTATTGTTTTCAGAGCCACATTTTGTTGCATATTTGCTAGTACTAATCAGTCAAAGGGC
ACCATTCTTTTTTTTTTTTTTTGAAACCAAAGCTGTCTCAGAAATGGCCAATTTAACTTTACAGTAACAATAGACAGCACAACACAAACTCTCTCAATAC
AGATAAACTCACACATACTGGAGATATATATATAATAGATATATATAAAATTATTTTAATGCATTGTAGTGTAATATTTATGCATACTATACTGTATAAC
ATGTTATTCAAAAGGGATTGCCATTTCTGAGACACAGTAACAAAAAAATGAGGAAATTATTTTGCTTCTATTTATAGCCTCTGTCAAAAGTCAAAAGACT
ATAAATGCTTTGCAAAAATGGTTTCACGTTTGCTTAAATGCTTCATCACAGTCACATTCAAAATAGTGACTCTAAACAAAGAAGAAAGCAGCACTGTCAT
CAGATGCATGATAAACCAAAATATGAAAATGGGAAATGTTTAATTAACCTAGTAATTGGGTGGGTTAAGTACATGGGTGAATTTTATATGTGATTTTTGT
TTTGTTTTGTTTTGTTCAGATTAACTGCTTATAGCCTTAGAAAGCCTTTTACAAAATTAAAAAAAAAATAGATGTGCATTCAGTTTTTAAGAATGGAATC
ATCCAAAGGAATTCCTTTTTTTGAGGTTTGGATGTTGCAGCTAGTAAAGGATATTTTTGCTCTGTTCAGCAGTTCTAAAAATTGCTGAAGTAGGGGCCAG
GTCACTGGTAGTTATAGTATGGAATGGGAGAAGTGAAAGTTCAGTTATAGAACTTTCCATACTTCCAAGTTTACTGCAAGTTTTTATGCTTGAGAGAGAT
GCTTTCTAATATAAGACTGATGTGTTGATTTTACTGATTGTACTGTACATCTATTAAAGCCTTAGATTATTACATTACGGGTTGGAACCCATACCAATGT
AATTTCAATCGTGTTAAGAAAGTAATGGTGACTTCACATGTTATTGTAGTTAGTTACATTATAGAATATTACTTATTTTTCTTGTTAAAATGTAGTTTTT
CATTTCCTACATTTATTAGATTTTCATTTTCTATTAACAATTGAATACCATTTCAGTTTATAGACTTGTTTTATTAGATTTTACCAATGAATTTTTCAAA
ATACAAAAAAAAGTAGTTTTTCCTTCATAACATACTCAGTTTTGAATTACATGTAGTGTCACATGAATATTCGTATTGTTAACTAAATGATTTATATTTT
ACTGATTTAATATTACAGTGTAAGAATGTCAGTCATTGTTAGTTCTTGTCTAGTTTTCATTAAAAGAACAAAGATCTTTTATATGGATATCTTATAAATA
TATAATCATTGCTAAGTAAGAAGTTAAGTTGTTGCTATCGCAACAATCCTGGCAGACAATTGAGTAATATTTTGATGATTTATTTTGTTTGTAATTAGTT
ATTATAAGAAGATCTAGATCCTAGATATTAGAATAAAATTTATTTTCTACTGTATCCATTTCAAATGTTAAAATATTGTTTAATATTTTTGAAATCCCTG
AGTATCAGGCCTTGTTATAAATAAGCTGCATAATCAATAAATAGAACAAGGGACTTTTTGTTGATAATCCAAATACTCAAAGTTTACGTAATGAAAATTA
TAGCGTGTGTGCAAACTCTTGAGGGTTGATTATGCTGCAATTTAGCATGTTGGAACGTCTAGGGAGAAGGTTGACTTTTTGCACTTCTGTATATAGTCAA
AAGAGAGAAACCTGTATAATAGTAAGATCTTATTTTGAATAAAAACGTCTATAATTACAAGGAGTTTTGTTAAGGCTAATACAATGACAGACTGAGCAAA
ATTGCTTGCAAAAGTGGCACAGAGTTAGCACTCCATACCCCTTCAAACATGTTGCTTTGCTTTCTTGTGGACAGCTTGTAGTTTGCCAGGATTTTTTCAG
CTGGAAAGATACGCCATCCTTTCAAACCCTCATGACTGACAAAAACTCCATGGGGCCAAATCTGCCTGAAGATCATTACCAAAAATAGCAGGTACTTCTA
CCATTAAGGTGAAATCATGGATCAGATATTCCTTACATTTTTCAAAACTACTGCATGTTTAAAACTTCAACAAAAAAAGAGAGAAAGAACTATACTAAGA
ACATATATTATTCAGATCAGTTTCTGCCAATTTCAGTGGTTTATTGTTCACAAAAAAATCTTCAAAACAAGTATTGACTTTCACAAAATTTAAATCATAA
ACAGGCAAACCAAACAGCACACTGTAGCTATAGTTGTTATGTGATTGTTTTTTAATTGCTGTAGGATCCTGTTCTTTCAGCAGGTGAAAAATAAAACGCA
GTTCAAATTTCATGGTTTTAATTTTCAACTCAGAAGCACTCAAAAATGCAAAATGTGATAATGGGCACTTGTTTAAAAGAATTAGTGTATCCAGCCTTCA
CTCCAGCTGGTTAAAAATGTTGCACTTATCAGCAACCCTACCACTTTCATCTGCTGAAAGGACAAATGTGCTTGGTTTTACTATTATGTAATCACAACTT
ACTTTCTGCTTGTAGTTGCTTAAAATTATGTATTTTGTCTTGGGCTGCAATTTGTTTTATGCTTATTTTATTATTACTGCAGTAGTTGACTTTGCTGTAT
GGAAAAATAAAGTGAAATTGCCCTAATAAAACTTCTCTTTCTTAAGTA
- refseq_MBNL1.F2 (forward primer)
- refseq_MBNL1.R2 (reverse primer)
- Single exon skipping
Frame 0
VGTKKCLS*RLCCLEPLLEQSLLLPCLLPARL*RQHIHPPPLAQTPPFLLIIKRKALSIWHCPRLL*
C*KKSLLKK*DIFCQEIKEEKKNHFLDFALNCC
ICLCQTNQYRLHHQTPLQIQL*GDSLSDNFAES
SLEIRCRRVCHVFMLAFWAPNWHWEGVTESTRLI
PGPTFKRSSTYNPSISLKTKTSLN*QFHAVNF*
WEIFSLILTTQFSMYF*SREWGKVF*GDIFIIS
SAFFFWLLLFFGGVGFVGFTETFNYL*NLNMAVS
VTPIRDTKWLTLEVCREFQRGTCSRPDTECKFA
HPSKSCQVENGRVIACFDSLKGRCSRENCKYLH
PPPHLKTQLEINGRNNLIQQKNMAMLAQQMQLAN
AMMPGAPLQPVVCREYQRGNCNRGENDCRFAHP
ADSTMIDTNDNTVTVCMDYIKGRCSREKCKYFH
PPAHLQAKIKAAQYQVNQAAAAQAAATAAAMGIP
QAVLPPLPKRPALEKTNGATAVFNTGIFQYQQA
LANMQLQQHTAFLPPGSILCMTPATSVVPMVHG
ATPATVSAATTSATSVPFAATATANQIPIISAEH
LTSHKYVTQM*NFHH*TIMLKRKGQCAWLE*RT
RSLAILYISSSNTHKSSSATRHPHIAC*PEEKT
TFSGNPLHTVAYTLCTFN*YLC*GDIPV*KNNIV
FLF*HRVMHSKMHT*LVSYIFMPS*KDRLWCNH
DSIMYWYVCRPRYNFLKISLFLSRFTNNKGAPC
I*NCHYR*ERACTVIFV*E*YF*CNRL*DVVREG
SDRDECAKQNHNCVYFKAHHGFKYHVVKDSHEV
P*TVHQIRVLFLQCYCFQSHILLHIC*Y*SVKG
HHSFFFFLKPKLSQKWPI*LYSNNRQHNTNSLNT
DKLTHTGDIYIIDIYKIILMHCSVIFMHTILYN
MLFKRDCHF*DTVTKK*GNYFASIYSLCQKSKD
YKCFAKMVSRLLKCFITVTFKIVTLNKEESSTVI
RCMINQNMKMGNV*LT**LGGLSTWVNFICDFC
FVLFCSD*LLIALESLLQN*KKNRCAFSF*EWN
HPKEFLFLRFGCCS**RIFLLCSAVLKIAEVGAR
SLVVIVWNGRSESSVIELSILPSLLQVFMLERD
AF*YKTDVLILLIVLYIY*SLRLLHYGLEPIPM
*FQSC*ESNGDFTCYCS*LHYRILLIFLVKM*FF
ISYIY*IFIFY*QLNTISVYRLVLLDFTNEFFK
IQKKVVFPS*HTQF*ITCSVT*IFVLLTK*FIF
Y*FNITV*ECQSLLVLV*FSLKEQRSFIWISYKY
IIIAK*EVKLLLSQQSWQTIE*YFDDLFCL*LV
IIRRSRS*ILE*NLFSTVSISNVKILFNIFEIP
EYQALL*ISCIINK*NKGLFVDNPNTQSLRNENY
SVCANS*GLIMLQFSMLERLGRRLTFCTSVYSQ
KRETCIIVRSYFE*KRL*LQGVLLRLIQ*QTEQ
NCLQKWHRVSTPYPFKHVALLSCGQLVVCQDFFS
WKDTPSFQTLMTDKNSMGPNLPEDHYQK*QVLL
PLR*NHGSDIPYIFQNYCMFKTSTKKERKNYTK
NIYYSDQFLPISVVYCSQKNLQNKY*LSQNLNHK
QANQTAHCSYSCYVIVF*LL*DPVLSAGEK*NA
VQISWF*FSTQKHSKMQNVIMGTCLKELVYPAF
TPAG*KCCTYQQPYHFHLLKGQMCLVLLLCNHNL
LSACSCLKLCILSWAAICFMLILLLLQ*LTLLY
GKIK*NCPNKTSLS*V
Frame 1
LVLRSAFPDVSAAWNRF*SSLCFCLACCQLDCDDSTSTLHL*PRHPHFYL*SREKL*VSGIALGCF
SVKRKVC*KSKISSARKSRRKKKIIFSILL*TAA
SVYAKLINTDCTTKLHCKFSCEEIPFQTTLLKA
AWKFGVEGSATFSCLHFGLQIGTGKGLLRAQG*
YQALLLNVHLLTILVFL*KPKPL*INSFML*ISS
GRSFP*Y*RHNFPCTFKAGSGEKYFEGTFSSSV
QLFFFGCCSFLGGLGLLVSLKHLTTCKI*TWLL
VSHQFGTQNG*HWKYVESSRGGLAHGQTRNVNLH
ILRKAAKLKMDE*SPALIH*KAVAPGRTANIFI
HPHI*KRSWR*MDAIT*FSRRTWPCWPSKCN*P
MP*CLVPHYNPWYVESTNVAIATEEKMIVGLLIL
LTAQ*LTPMTTQSLCVWITSKGDALGKSANTFI
PLHICKPRSRLPNTRSTRLQLHRLQPPQLPWEF
LKLYFPHYQRGLLLKKPTVPPQSLTLVFSNTNRL
*PTCSYNSIQHFSHQAQYCA*HPLQVLFPWCTV
LRQPLCPQQQHLPQVFPSLQQPQPTRYP*YLPN
I*LATSMLPRCRIFITKQSC*RGKDSVLG*SKGR
GH*PYCIYRQATHTKVPQPQDIHILHVNQKKRQ
HFPEIHCTLLPIHFVHLIDICAEVIFLSKRTTL
SFFSSTELCIQRCIPS*FPIYSCHLEKTDYGVTM
ILLCIGTSVDQDIIF*K*VYFFQGLQITKVHLV
FKIAIIDESVHAQSFLFKSNIFNVIDCKTW*GR
DLTEMNVPSKTTTVYILKHIMALSTMLLRILMKC
HRLYIKLEYYFFSVIVFRATFCCIFASTNQSKG
TILFFFF*NQSCLRNGQFNFTVTIDSTTQTLSI
QINSHILEIYI**IYIKLF*CIVV*YLCILYCIT
CYSKGIAISETQ*QKNEEIILLLFIASVKSQKT
INALQKWFHVCLNASSQSHSK**L*TKKKAALS
SDA**TKI*KWEMFN*PSNWVG*VHG*ILYVIFV
LFCFVQINCL*P*KAFYKIKKKIDVHSVFKNGI
IQRNSFF*GLDVAASKGYFCSVQQF*KLLK*GP
GHW*L*YGMGEVKVQL*NFPYFQVYCKFLCLREM
LSNIRLMC*FY*LYCTSIKALDYYITGWNPYQC
NFNRVKKVMVTSHVIVVSYIIEYYLFFLLKCSF
SFPTFIRFSFSINN*IPFQFIDLFY*ILPMNFSK
YKKK*FFLHNILSFELHVVSHEYSYC*LNDLYF
TDLILQCKNVSHC*FLSSFH*KNKDLLYGYLIN
I*SLLSKKLSCCYRNNPGRQLSNILMIYFVCN*L
L*EDLDPRY*NKIYFLLYPFQMLKYCLIFLKSL
SIRPCYK*AA*SINRTRDFLLIIQILKVYVMKI
IACVQTLEG*LCCNLACWNV*GEG*LFALLYIVK
REKPV***DLILNKNVYNYKEFC*G*YNDRLSK
IACKSGTELALHTPSNMLLCFLVDSL*FARIFS
AGKIRHPFKPS*LTKTPWGQICLKIITKNSRYFY
H*GEIMDQIFLTFFKTTACLKLQQKKRERTILR
TYIIQISFCQFQWFIVHKKIFKTSIDFHKI*II
NRQTKQHTVAIVVM*LFFNCCRILFFQQVKNKTQ
FKFHGFNFQLRSTQKCKM**WALV*KN*CIQPS
LQLVKNVALISNPTTFIC*KDKCAWFYYYVITT
YFLLVVA*NYVFCLGLQFVLCLFYYYCSS*LCCM
EK*SEIALIKLLFLK
Frame 2
WY*EVPFLTSLLLGTASRAVSAFALLAAS*TVTTAHPPSTSSPDTPISTYNQEKSSKYLALP*AAL
VLKEKFAEKVRYLLPGNQGGKKKSFSRFCSKLL
HLSMPN*SIPIAPPNSIANSAVRRFPFRQLC*KQ
LGNSVSKGLPRFHACILGSKLALGRGY*EHKAD
TRPYF*TFIYLQS*YFSKNQNLFELTVSCCEFL
VGDLFLDIDDTIFHVLLKQGVGKSILRGHFHHQF
SFFFLVVALFWGGWVCWFH*NI*LPVKSKHGC*
CHTNSGHKMANTGSM*RVPEGDLLTARHGM*IC
TSFEKLPS*KWTSNRLL*FIERPLLQGELQISSS
TPTFKNAVGDKWTQ*LDSAEEHGHVGPANATSQ
CHDAWCPITTRGMSRVPTWQLQPRRK*LSVCSS
C*QHND*HQ*QHSHCVYGLHQREMLSGKVQILSS
PCTFASQDQGCPIPGQPGCSCTGCSHRSCHGNS
SSCTSPITKEACS*KNQRCHRSL*HWYFPIPTG
SSQHAVTTAYSISPTRLNIVHDTRYKCCSHGARC
YASHCVRSNNICHKCSLRCNSHSQPDTHNICRT
SD*PQVCYPDVEFSSLNNHAKEERTVCLVRVKD
EVISHIVYIVKQHTQKFLSHKTSTYCMLTRRKDN
IFRKSTAHCCLYTLYI*LIFVLR*YSCLKEQHC
LSFLAQSYAFKDAYLVSFLYIHAILKRQTMV*P
*FYYVLVRL*TKI*FFKNKFISFKVYK*QRCTLY
LKLPL*MRACMHSHFCLRVIFLM**IVRRGEGG
I*QR*MCQAKPQLCIF*STSWL*VPCC*GFS*S
AIDCTSN*SIISSVLLFSEPHFVAYLLVLISQRA
PFFFFFFETKAVSEMANLTLQ*Q*TAQHKLSQY
R*THTYWRYIYNRYI*NYFNAL*CNIYAYYTV*
HVIQKGLPFLRHSNKKMRKLFCFYL*PLSKVKRL
*MLCKNGFTFA*MLHHSHIQNSDSKQRRKQHCH
QMHDKPKYENGKCLINLVIGWVKYMGEFYM*FL
FCFVLFRLTAYSLRKPFTKLKKK*MCIQFLRMES
SKGIPFFEVWMLQLVKDIFALFSSSKNC*SRGQ
VTGSYSMEWEK*KFSYRTFHTSKFTASFYA*ER
CFLI*D*CVDFTDCTVHLLKP*IITLRVGTHTNV
ISIVLRK*W*LHMLL*LVTL*NITYFSC*NVVF
HFLHLLDFHFLLTIEYHFSL*TCFIRFYQ*IFQ
NTKKSSFSFITYSVLNYM*CHMNIRIVN*MIYIL
LI*YYSVRMSVIVSSCLVFIKRTKIFYMDIL*I
YNHC*VRS*VVAIATILADN*VIF**FILFVIS
YYKKI*ILDIRIKFIFYCIHFKC*NIV*YF*NP*
VSGLVINKLHNQ*IEQGTFC**SKYSKFT**KL
*RVCKLLRVDYAAI*HVGTSREKVDFLHFCI*S
KERNLYNSKILF*IKTSIITRSFVKANTMTD*AK
LLAKVAQS*HSIPLQTCCFAFLWTACSLPGFFQ
LERYAILSNPHD*QKLHGAKSA*RSLPKIAGTS
TIKVKSWIRYSLHFSKLLHV*NFNKKREKELY*E
HILFRSVSANFSGLLFTKKSSKQVLTFTKFKS*
TGKPNSTL*L*LLCDCFLIAVGSCSFSR*KIKR
SSNFMVLIFNSEALKNAKCDNGHLFKRISVSSLH
SSWLKMLHLSATLPLSSAERTNVLGFTIM*SQL
TFCL*LLKIMYFVLGCNLFYAYFIITAVVDFAV
WKNKVKLP**NFSFLS
Sequence NM_021038
TTACATCTTATAGCACCCAGTGAATTATGATATGGGGCTGCGGTTTATTTGCTCTGGCGACAATATGGCTTACTTGCCGTGGAATATCCCGGTATTTCAGAAGTAACATATTATTTTTAAAGAGGGAGCTTTGCTTTCTCTAAATCACATGGTGCAAAGTTGGGAATACAGTGGCCTGTAAATTACAAATATTATTTACA
AACTAGAAGGAAAGCTGACATGTGTTTGTGCCTGTGTGACAGTTTCACTAAGGAATTCCAAAACTGTGGATAGCCTGTTACTTGAGAAAAATACACGGTG
GCAGTCTTGCAGATGTTTTTCCCGCCTGTTAGCCCTCCAGGTTCAAAAACTGTTTGGTGCATTTTAAGGCGGCAGTCTCACCAGATCGCAGGATTCTAGT
CTAATTGGCTTCTAACCTTCAATCTAACCTTGCCGGCTTCCTTGCAAAGCCCGGTGCAAGGGCCTCTTTCAAAATGAACCCACTGGTGTGCCTAGCAGTC
GGTAGAAGAAGCGGGAGGGCGTCCGGTCTGCACGCCCGCCGCGAGGTTACAATGCTGAACGCATGAGATGGAAGATACCAACGGGAGGCCGAGGGGATCC
ACGGCGCCCGCGCGGGCTCCGGCTTCCTCCTGCTCTCGGCGCCGCTGGGCGACCGCCCATGACCCGCTCTTGCGGGCTCTGTCCGGTTGACAGGCGACCC
TGTGGCCCGGGGAAGCGCGGGAGGGCGCCGGCGGAAAGTTGAAGAGCGTTTTTCTCGCCGCCGCGTGCATTAGGAGCTCGACGAGTCCGCCCTGGGCTTC
CTGGTGGGGCTGGGCGGGCGGGGGAGGGGCCGCGCAGCAGCAGCGGAAGCCAGACCTCGGCGATAAGAGGCTGCACAGCGACATGCAACAGTCTTTTCAC
TGCAGCTGAATGAGTTGTGGCGCCCACAATGCTCCCATGACAAGGAGCTGACAAGTTCCATTTTCCGTCGCGGGCATCTTGGAATCATGACTCCCACAAT
GCCTTGGGCACTTGGTCGACAGTGGGGCCGCCTCTGAAAAAAAAATGTGAGAGGTTGGTACTAAGAAGTGCCTTTCCTGACGTCTCTGCTGCTTGGAACC
GCTTCTAGAGCAGTCTCTGCTTTTGCCTTGCTTGCTGCCAGCTAGACTGTGACGACAGCACATCCACCCTCCACCTCTAGCCCAGACACCCCCATTTCTA
CTTATAATCAAGAGAAAAGCTCTAAGTATCTGGCATTGCCCTAGGCTGCTTTAGTGTTAAAAGAAAAGTTTGCTGAAAAAGTAAGATATCTTCTGCCAGG
AAATCAAGGAGGAAAAAAAAAATCATTTTCTCGATTTTGCTCTAAACTGCTGCATCTGTCTATGCCAAACTAATCAATACCGATTGCACCACCAAACTCC
ATTGCAAATTCAGCTGTGAGGAGATTCCCTTTCAGACAACTTTGCTGAAAGCAGCTTGGAAATTCGGTGTCGAAGGGTCTGCCACGTTTTCATGCTTGCA
TTTTGGGCTCCAAATTGGCACTGGGAAGGGGTTACTGAGAGCACAAGGCTGATACCAGGCCCTACTTTTAAACGTTCATCTACTTACAATCCTAGTATTT
CTCTAAAAACCAAAACCTCTTTGAATTAACAGTTTCATGCTGTGAATTTCTAGTGGGAGATCTTTTCCTTGATATTGACGACACAATTTTCCATGTACTT
TTAAAGCAGGGAGTGGGGAAAAGTATTTTGAGGGGACATTTTCATCATCAGTTCAGCTTTTTTTTTTTGGTTGTTGCTCTTTTTTGGGGGGGTTGGGTTT
GTTGGTTTCACTGAAACATTTAACTACCTGTAAAATCTAAACATGGCTGTTAGTGTCACACCAATTCGGGACACAAAATGGCTAACACTGGAAGTATGTA
GAGAGTTCCAGAGGGGGACTTGCTCACGGCCAGACACGGAATGTAAATTTGCACATCCTTCGAAAAGCTGCCAAGTTGAAAATGGACGAGTAATCGCCTG
CTTTGATTCATTGAAAGGCCGTTGCTCCAGGGAGAACTGCAAATATCTTCATCCACCCCCACATTTAAAAACGCAGTTGGAGATAAATGGACGCAATAAC
TTGATTCAGCAGAAGAACATGGCCATGTTGGCCCAGCAAATGCAACTAGCCAATGCCATGATGCCTGGTGCCCCATTACAACCCGTGCCAATGTTTTCAG
TTGCACCAAGCTTAGCCACCAATGCATCAGCAGCCGCCTTTAATCCCTATCTGGGACCTGTTTCTCCAAGCCTGGTCCCGGCAGAGATCTTGCCGACTGC
ACCAATGTTGGTTACAGGGAATCCGGGTGTCCCTGTACCTGCAGCTGCTGCAGCTGCTGCACAGAAATTAATGCGAACAGACAGACTTGAGGTATGTCGA
GAGTACCAACGTGGCAATTGCAACCGAGGAGAAAATGATTGTCGGTTTGCTCATCCTGCTGACAGCACAATGATTGACACCAATGACAACACAGTCACTG
TGTGTATGGATTACATCAAAGGGAGATGCTCTCGGGAAAAGTGCAAATACTTTCATCCCCCTGCACATTTGCAAGCCAAGATCAAGGCTGCCCAATACCA
GGTCAACCAGGCTGCAGCTGCACAGGCTGCAGCCACCGCAGCTGCCATGGGAATTCCTCAAGCTGTACTTCCCCCATTACCAAAGAGGCCTGCTCTTGAA
AAAACCAACGGTGCCACCGCAGTCTTTAACACTGGTATTTTCCAATACCAACAGGCTCTAGCCAACATGCAGTTACAACAGCATACAGCATTTCTCCCAC
CAGGCTCAATATTGTGCATGACACCCGCTACAAGTGTTGTTCCCATGGTGCACGGTGCTACGCCAGCCACTGTGTCCGCAGCAACAACATCTGCCACAAG
TGTTCCCTTCGCTGCAACAGCCACAGCCAACCAGATACCCATAATATCTGCCGAACATCTGACTAGCCACAAGTATGTTACCCAGATGTAGAATTTTCAT
CACTAAACAATCATGCTAAAGAGGAAAGGACAGTGTGCTTGGTTAGAGTAAAGGACGAGGTCATTAGCCATATTGTATATATCGTCAAGCAACACACACA
AAAGTTCCTCAGCCACAAGACATCCACATATTGCATGTTAACCAGAAGAAAAGACAACATTTTCCGGAAATCCACTGCACACTGTTGCCTATACACTTTG
TACATTTAATTGATATTTGTGCTGAGGTGATATTCCTGTCTAAAAGAACAACATTGTCTTTCTTTTCTAGCACAGAGTTATGCATTCAAAGATGCATACC
TAGTTAGTTTCCTATATATTCATGCCATCTTGAAAAGACAGACTATGGTGTAACCATGATTCTATTATGTATTGGTACGTCTGTAGACCAAGATATAATT
TTTTAAAAATAAGTTTATTTCTTTCAAGGTTTACAAATAACAAAGGTGCACCTTGTATTTAAAATTGCCATTATAGATGAGAGCGTGCATGCACAGTCAT
TTTTGTTTAAGAGTAATATTTTTAATGTAATAGATTGTAAGACGTGGTGAGGGAGGGATCTGACAGAGATGAATGTGCCAAGCAAAACCACAACTGTGTA
TATTTTAAAGCACATCATGGCTTTAAGTACCATGTTGTTAAGGATTCTCATGAAGTGCCATAGACTGTACATCAAATTAGAGTATTATTTCTTCAGTGTT
ATTGTTTTCAGAGCCACATTTTGTTGCATATTTGCTAGTACTAATCAGTCAAAGGGCACCATTCTTTTTTTTTTTTTTTGAAACCAAAGCTGTCTCAGAA
ATGGCCAATTTAACTTTACAGTAACAATAGACAGCACAACACAAACTCTCTCAATACAGATAAACTCACACATACTGGAGATATATATATAATAGATATA
TATAAAATTATTTTAATGCATTGTAGTGTAATATTTATGCATACTATACTGTATAACATGTTATTCAAAAGGGATTGCCATTTCTGAGACACAGTAACAA
AAAAATGAGGAAATTATTTTGCTTCTATTTATAGCCTCTGTCAAAAGTCAAAAGACTATAAATGCTTTGCAAAAATGGTTTCACGTTTGCTTAAATGCTT
CATCACAGTCACATTCAAAATAGTGACTCTAAACAAAGAAGAAAGCAGCACTGTCATCAGATGCATGATAAACCAAAATATGAAAATGGGAAATGTTTAA
TTAACCTAGTAATTGGGTGGGTTAAGTACATGGGTGAATTTTATATGTGATTTTTGTTTTGTTTTGTTTTGTTCAGATTAACTGCTTATAGCCTTAGAAA
GCCTTTTACAAAATTAAAAAAAAAATAGATGTGCATTCAGTTTTTAAGAATGGAATCATCCAAAGGAATTCCTTTTTTTGAGGTTTGGATGTTGCAGCTA
GTAAAGGATATTTTTGCTCTGTTCAGCAGTTCTAAAAATTGCTGAAGTAGGGGCCAGGTCACTGGTAGTTATAGTATGGAATGGGAGAAGTGAAAGTTCA
GTTATAGAACTTTCCATACTTCCAAGTTTACTGCAAGTTTTTATGCTTGAGAGAGATGCTTTCTAATATAAGACTGATGTGTTGATTTTACTGATTGTAC
TGTACATCTATTAAAGCCTTAGATTATTACATTACGGGTTGGAACCCATACCAATGTAATTTCAATCGTGTTAAGAAAGTAATGGTGACTTCACATGTTA
TTGTAGTTAGTTACATTATAGAATATTACTTATTTTTCTTGTTAAAATGTAGTTTTTCATTTCCTACATTTATTAGATTTTCATTTTCTATTAACAATTG
AATACCATTTCAGTTTATAGACTTGTTTTATTAGATTTTACCAATGAATTTTTCAAAATACAAAAAAAAGTAGTTTTTCCTTCATAACATACTCAGTTTT
GAATTACATGTAGTGTCACATGAATATTCGTATTGTTAACTAAATGATTTATATTTTACTGATTTAATATTACAGTGTAAGAATGTCAGTCATTGTTAGT
TCTTGTCTAGTTTTCATTAAAAGAACAAAGATCTTTTATATGGATATCTTATAAATATATAATCATTGCTAAGTAAGAAGTTAAGTTGTTGCTATCGCAA
CAATCCTGGCAGACAATTGAGTAATATTTTGATGATTTATTTTGTTTGTAATTAGTTATTATAAGAAGATCTAGATCCTAGATATTAGAATAAAATTTAT
TTTCTACTGTATCCATTTCAAATGTTAAAATATTGTTTAATATTTTTGAAATCCCTGAGTATCAGGCCTTGTTATAAATAAGCTGCATAATCAATAAATA
GAACAAGGGACTTTTTGTTGATAATCCAAATACTCAAAGTTTACGTAATGAAAATTATAGCGTGTGTGCAAACTCTTGAGGGTTGATTATGCTGCAATTT
AGCATGTTGGAACGTCTAGGGAGAAGGTTGACTTTTTGCACTTCTGTATATAGTCAAAAGAGAGAAACCTGTATAATAGTAAGATCTTATTTTGAATAAA
AACGTCTATAATTACAAGGAGTTTTGTTAAGGCTAATACAATGACAGACTGAGCAAAATTGCTTGCAAAAGTGGCACAGAGTTAGCACTCCATACCCCTT
CAAACATGTTGCTTTGCTTTCTTGTGGACAGCTTGTAGTTTGCCAGGATTTTTTCAGCTGGAAAGATACGCCATCCTTTCAAACCCTCATGACTGACAAA
AACTCCATGGGGCCAAATCTGCCTGAAGATCATTACCAAAAATAGCAGGTACTTCTACCATTAAGGTGAAATCATGGATCAGATATTCCTTACATTTTTC
AAAACTACTGCATGTTTAAAACTTCAACAAAAAAAGAGAGAAAGAACTATACTAAGAACATATATTATTCAGATCAGTTTCTGCCAATTTCAGTGGTTTA
TTGTTCACAAAAAAATCTTCAAAACAAGTATTGACTTTCACAAAATTTAAATCATAAACAGGCAAACCAAACAGCACACTGTAGCTATAGTTGTTATGTG
ATTGTTTTTTAATTGCTGTAGGATCCTGTTCTTTCAGCAGGTGAAAAATAAAACGCAGTTCAAATTTCATGGTTTTAATTTTCAACTCAGAAGCACTCAA
AAATGCAAAATGTGATAATGGGCACTTGTTTAAAAGAATTAGTGTATCCAGCCTTCACTCCAGCTGGTTAAAAATGTTGCACTTATCAGCAACCCTACCA
CTTTCATCTGCTGAAAGGACAAATGTGCTTGGTTTTACTATTATGTAATCACAACTTACTTTCTGCTTGTAGTTGCTTAAAATTATGTATTTTGTCTTGG
GCTGCAATTTGTTTTATGCTTATTTTATTATTACTGCAGTAGTTGACTTTGCTGTATGGAAAAATAAAGTGAAATTGCCCTAATAAAACTTCTCTTTCTT
AAGTA
- refseq_MBNL1.F2 (forward primer)
- refseq_MBNL1.R2 (reverse primer)
- Single exon skipping
Frame 0
LHLIAPSEL*YGAAVYLLWRQYGLLAVEYPGISEVTYYF*RGSFAFSKSHGAKLGIQWPVNYKYYLQ
TRRKADMCLCLCDSFTKEFQNCG*PVT*EKYTV
AVLQMFFPPVSPPGSKTVWCILRRQSHQIAGF*
SNWLLTFNLTLPASLQSPVQGPLSK*THWCA*QS
VEEAGGRPVCTPAARLQC*THEMEDTNGRPRGS
TAPARAPASSCSRRRWATAHDPLLRALSG*QAT
LWPGEAREGAGGKLKSVFLAAACIRSSTSPPWAS
WWGWAGGGGAAQQQRKPDLGDKRLHSDMQQSFH
CS*MSCGAHNAPMTRS*QVPFSVAGILES*LPQ
CLGHLVDSGAASEKKM*EVGTKKCLS*RLCCLEP
LLEQSLLLPCLLPARL*RQHIHPPPLAQTPPFL
LIIKRKALSIWHCPRLL*C*KKSLLKK*DIFCQ
EIKEEKKNHFLDFALNCCICLCQTNQYRLHHQTP
LQIQL*GDSLSDNFAESSLEIRCRRVCHVFMLA
FWAPNWHWEGVTESTRLIPGPTFKRSSTYNPSI
SLKTKTSLN*QFHAVNF*WEIFSLILTTQFSMYF
*SREWGKVF*GDIFIISSAFFFWLLLFFGGVGF
VGFTETFNYL*NLNMAVSVTPIRDTKWLTLEVC
REFQRGTCSRPDTECKFAHPSKSCQVENGRVIAC
FDSLKGRCSRENCKYLHPPPHLKTQLEINGRNN
LIQQKNMAMLAQQMQLANAMMPGAPLQPVPMFS
VAPSLATNASAAAFNPYLGPVSPSLVPAEILPTA
PMLVTGNPGVPVPAAAAAAAQKLMRTDRLEVCR
EYQRGNCNRGENDCRFAHPADSTMIDTNDNTVT
VCMDYIKGRCSREKCKYFHPPAHLQAKIKAAQYQ
VNQAAAAQAAATAAAMGIPQAVLPPLPKRPALE
KTNGATAVFNTGIFQYQQALANMQLQQHTAFLP
PGSILCMTPATSVVPMVHGATPATVSAATTSATS
VPFAATATANQIPIISAEHLTSHKYVTQM*NFH
H*TIMLKRKGQCAWLE*RTRSLAILYISSSNTH
KSSSATRHPHIAC*PEEKTTFSGNPLHTVAYTLC
TFN*YLC*GDIPV*KNNIVFLF*HRVMHSKMHT
*LVSYIFMPS*KDRLWCNHDSIMYWYVCRPRYN
FLKISLFLSRFTNNKGAPCI*NCHYR*ERACTVI
FV*E*YF*CNRL*DVVREGSDRDECAKQNHNCV
YFKAHHGFKYHVVKDSHEVP*TVHQIRVLFLQC
YCFQSHILLHIC*Y*SVKGHHSFFFFLKPKLSQK
WPI*LYSNNRQHNTNSLNTDKLTHTGDIYIIDI
YKIILMHCSVIFMHTILYNMLFKRDCHF*DTVT
KK*GNYFASIYSLCQKSKDYKCFAKMVSRLLKCF
ITVTFKIVTLNKEESSTVIRCMINQNMKMGNV*
LT**LGGLSTWVNFICDFCFVLFCSD*LLIALE
SLLQN*KKNRCAFSF*EWNHPKEFLFLRFGCCS*
*RIFLLCSAVLKIAEVGARSLVVIVWNGRSESS
VIELSILPSLLQVFMLERDAF*YKTDVLILLIV
LYIY*SLRLLHYGLEPIPM*FQSC*ESNGDFTCY
CS*LHYRILLIFLVKM*FFISYIY*IFIFY*QL
NTISVYRLVLLDFTNEFFKIQKKVVFPS*HTQF
*ITCSVT*IFVLLTK*FIFY*FNITV*ECQSLLV
LV*FSLKEQRSFIWISYKYIIIAK*EVKLLLSQ
QSWQTIE*YFDDLFCL*LVIIRRSRS*ILE*NL
FSTVSISNVKILFNIFEIPEYQALL*ISCIINK*
NKGLFVDNPNTQSLRNENYSVCANS*GLIMLQF
SMLERLGRRLTFCTSVYSQKRETCIIVRSYFE*
KRL*LQGVLLRLIQ*QTEQNCLQKWHRVSTPYPF
KHVALLSCGQLVVCQDFFSWKDTPSFQTLMTDK
NSMGPNLPEDHYQK*QVLLPLR*NHGSDIPYIF
QNYCMFKTSTKKERKNYTKNIYYSDQFLPISVVY
CSQKNLQNKY*LSQNLNHKQANQTAHCSYSCYV
IVF*LL*DPVLSAGEK*NAVQISWF*FSTQKHS
KMQNVIMGTCLKELVYPAFTPAG*KCCTYQQPYH
FHLLKGQMCLVLLLCNHNLLSACSCLKLCILSW
AAICFMLILLLLQ*LTLLYGKIK*NCPNKTSLS
*V
Frame 1
YIL*HPVNYDMGLRFICSGDNMAYLPWNIPVFQK*HIIFKEGALLSLNHMVQSWEYSGL*ITNIIY
KLEGKLTCVCACVTVSLRNSKTVDSLLLEKNTRW
QSCRCFSRLLALQVQKLFGAF*GGSLTRSQDSS
LIGF*PSI*PCRLPCKARCKGLFQNEPTGVPSS
R*KKREGVRSARPPRGYNAERMRWKIPTGGRGDP
RRPRGLRLPPALGAAGRPPMTRSCGLCPVDRRP
CGPGKRGRAPAES*RAFFSPPRALGARRVRPGL
PGGAGRAGEGPRSSSGSQTSAIRGCTATCNSLFT
AAE*VVAPTMLP*QGADKFHFPSRASWNHDSHN
ALGTWSTVGPPLKKKCERLVLRSAFPDVSAAWN
RF*SSLCFCLACCQLDCDDSTSTLHL*PRHPHFY
L*SREKL*VSGIALGCFSVKRKVC*KSKISSAR
KSRRKKKIIFSILL*TAASVYAKLINTDCTTKL
HCKFSCEEIPFQTTLLKAAWKFGVEGSATFSCLH
FGLQIGTGKGLLRAQG*YQALLLNVHLLTILVF
L*KPKPL*INSFML*ISSGRSFP*Y*RHNFPCT
FKAGSGEKYFEGTFSSSVQLFFFGCCSFLGGLGL
LVSLKHLTTCKI*TWLLVSHQFGTQNG*HWKYV
ESSRGGLAHGQTRNVNLHILRKAAKLKMDE*SP
ALIH*KAVAPGRTANIFIHPHI*KRSWR*MDAIT
*FSRRTWPCWPSKCN*PMP*CLVPHYNPCQCFQ
LHQA*PPMHQQPPLIPIWDLFLQAWSRQRSCRL
HQCWLQGIRVSLYLQLLQLLHRN*CEQTDLRYVE
STNVAIATEEKMIVGLLILLTAQ*LTPMTTQSL
CVWITSKGDALGKSANTFIPLHICKPRSRLPNT
RSTRLQLHRLQPPQLPWEFLKLYFPHYQRGLLLK
KPTVPPQSLTLVFSNTNRL*PTCSYNSIQHFSH
QAQYCA*HPLQVLFPWCTVLRQPLCPQQQHLPQ
VFPSLQQPQPTRYP*YLPNI*LATSMLPRCRIFI
TKQSC*RGKDSVLG*SKGRGH*PYCIYRQATHT
KVPQPQDIHILHVNQKKRQHFPEIHCTLLPIHF
VHLIDICAEVIFLSKRTTLSFFSSTELCIQRCIP
S*FPIYSCHLEKTDYGVTMILLCIGTSVDQDII
F*K*VYFFQGLQITKVHLVFKIAIIDESVHAQS
FLFKSNIFNVIDCKTW*GRDLTEMNVPSKTTTVY
ILKHIMALSTMLLRILMKCHRLYIKLEYYFFSV
IVFRATFCCIFASTNQSKGTILFFFF*NQSCLR
NGQFNFTVTIDSTTQTLSIQINSHILEIYI**IY
IKLF*CIVV*YLCILYCITCYSKGIAISETQ*Q
KNEEIILLLFIASVKSQKTINALQKWFHVCLNA
SSQSHSK**L*TKKKAALSSDA**TKI*KWEMFN
*PSNWVG*VHG*ILYVIFVLFCFVQINCL*P*K
AFYKIKKKIDVHSVFKNGIIQRNSFF*GLDVAA
SKGYFCSVQQF*KLLK*GPGHW*L*YGMGEVKVQ
L*NFPYFQVYCKFLCLREMLSNIRLMC*FY*LY
CTSIKALDYYITGWNPYQCNFNRVKKVMVTSHV
IVVSYIIEYYLFFLLKCSFSFPTFIRFSFSINN*
IPFQFIDLFY*ILPMNFSKYKKK*FFLHNILSF
ELHVVSHEYSYC*LNDLYFTDLILQCKNVSHC*
FLSSFH*KNKDLLYGYLINI*SLLSKKLSCCYRN
NPGRQLSNILMIYFVCN*LL*EDLDPRY*NKIY
FLLYPFQMLKYCLIFLKSLSIRPCYK*AA*SIN
RTRDFLLIIQILKVYVMKIIACVQTLEG*LCCNL
ACWNV*GEG*LFALLYIVKREKPV***DLILNK
NVYNYKEFC*G*YNDRLSKIACKSGTELALHTP
SNMLLCFLVDSL*FARIFSAGKIRHPFKPS*LTK
TPWGQICLKIITKNSRYFYH*GEIMDQIFLTFF
KTTACLKLQQKKRERTILRTYIIQISFCQFQWF
IVHKKIFKTSIDFHKI*IINRQTKQHTVAIVVM*
LFFNCCRILFFQQVKNKTQFKFHGFNFQLRSTQ
KCKM**WALV*KN*CIQPSLQLVKNVALISNPT
TFIC*KDKCAWFYYYVITTYFLLVVA*NYVFCLG
LQFVLCLFYYYCSS*LCCMEK*SEIALIKLLFL
K
Frame 2
TSYSTQ*IMIWGCGLFALATIWLTCRGISRYFRSNILFLKRELCFL*ITWCKVGNTVACKLQILFT
N*KES*HVFVPV*QFH*GIPKLWIACYLRKIHG
GSLADVFPAC*PSRFKNCLVHFKAAVSPDRRILV
*LASNLQSNLAGFLAKPGARASFKMNPLVCLAV
GRRSGRASGLHARREVTMLNA*DGRYQREAEGI
HGARAGSGFLLLSAPLGDRP*PALAGSVRLTGDP
VARGSAGGRRRKVEERFSRRRVH*ELDESALGF
LVGLGGRGRGRAAAAEARPRR*EAAQRHATVFS
LQLNELWRPQCSHDKELTSSIFRRGHLGIMTPTM
PWALGRQWGRL*KKNVRGWY*EVPFLTSLLLGT
ASRAVSAFALLAAS*TVTTAHPPSTSSPDTPIS
TYNQEKSSKYLALP*AALVLKEKFAEKVRYLLPG
NQGGKKKSFSRFCSKLLHLSMPN*SIPIAPPNS
IANSAVRRFPFRQLC*KQLGNSVSKGLPRFHAC
ILGSKLALGRGY*EHKADTRPYF*TFIYLQS*YF
SKNQNLFELTVSCCEFLVGDLFLDIDDTIFHVL
LKQGVGKSILRGHFHHQFSFFFLVVALFWGGWV
CWFH*NI*LPVKSKHGC*CHTNSGHKMANTGSM*
RVPEGDLLTARHGM*ICTSFEKLPS*KWTSNRL
L*FIERPLLQGELQISSSTPTFKNAVGDKWTQ*
LDSAEEHGHVGPANATSQCHDAWCPITTRANVFS
CTKLSHQCISSRL*SLSGTCFSKPGPGRDLADC
TNVGYRESGCPCTCSCCSCCTEINANRQT*GMS
RVPTWQLQPRRK*LSVCSSC*QHND*HQ*QHSHC
VYGLHQREMLSGKVQILSSPCTFASQDQGCPIP
GQPGCSCTGCSHRSCHGNSSSCTSPITKEACS*
KNQRCHRSL*HWYFPIPTGSSQHAVTTAYSISPT
RLNIVHDTRYKCCSHGARCYASHCVRSNNICHK
CSLRCNSHSQPDTHNICRTSD*PQVCYPDVEFS
SLNNHAKEERTVCLVRVKDEVISHIVYIVKQHTQ
KFLSHKTSTYCMLTRRKDNIFRKSTAHCCLYTL
YI*LIFVLR*YSCLKEQHCLSFLAQSYAFKDAY
LVSFLYIHAILKRQTMV*P*FYYVLVRL*TKI*F
FKNKFISFKVYK*QRCTLYLKLPL*MRACMHSH
FCLRVIFLM**IVRRGEGGI*QR*MCQAKPQLC
IF*STSWL*VPCC*GFS*SAIDCTSN*SIISSVL
LFSEPHFVAYLLVLISQRAPFFFFFFETKAVSE
MANLTLQ*Q*TAQHKLSQYR*THTYWRYIYNRY
I*NYFNAL*CNIYAYYTV*HVIQKGLPFLRHSNK
KMRKLFCFYL*PLSKVKRL*MLCKNGFTFA*ML
HHSHIQNSDSKQRRKQHCHQMHDKPKYENGKCL
INLVIGWVKYMGEFYM*FLFCFVLFRLTAYSLRK
PFTKLKKK*MCIQFLRMESSKGIPFFEVWMLQL
VKDIFALFSSSKNC*SRGQVTGSYSMEWEK*KF
SYRTFHTSKFTASFYA*ERCFLI*D*CVDFTDCT
VHLLKP*IITLRVGTHTNVISIVLRK*W*LHML
L*LVTL*NITYFSC*NVVFHFLHLLDFHFLLTI
EYHFSL*TCFIRFYQ*IFQNTKKSSFSFITYSVL
NYM*CHMNIRIVN*MIYILLI*YYSVRMSVIVS
SCLVFIKRTKIFYMDIL*IYNHC*VRS*VVAIA
TILADN*VIF**FILFVISYYKKI*ILDIRIKFI
FYCIHFKC*NIV*YF*NP*VSGLVINKLHNQ*I
EQGTFC**SKYSKFT**KL*RVCKLLRVDYAAI
*HVGTSREKVDFLHFCI*SKERNLYNSKILF*IK
TSIITRSFVKANTMTD*AKLLAKVAQS*HSIPL
QTCCFAFLWTACSLPGFFQLERYAILSNPHD*Q
KLHGAKSA*RSLPKIAGTSTIKVKSWIRYSLHFS
KLLHV*NFNKKREKELY*EHILFRSVSANFSGL
LFTKKSSKQVLTFTKFKS*TGKPNSTL*L*LLC
DCFLIAVGSCSFSR*KIKRSSNFMVLIFNSEALK
NAKCDNGHLFKRISVSSLHSSWLKMLHLSATLP
LSSAERTNVLGFTIM*SQLTFCL*LLKIMYFVL
GCNLFYAYFIITAVVDFAVWKNKVKLP**NFSFL
S
Copyright ©2008,2017, all rights reserved. 