Sequence XM_001126088
CTCGCCAGCCGCGGGTTCGGGCGTCTTCGGACCAGCGGGGCGCCGCAGGCCCCTCGCAGCGTCCGTCGGCAGGCGGGCAGACGGGCGGGGGAGTCGCCCC
GGCGGGGCAAGTCCGTACCGCGACATGGGCGCGCCGAGCACGTCCGTACCGCAAGATGGCTGCTCGGACGGGGACAGAGCTCGCCTCTGCCGCCTCGACA
ACTGCTCCTGGGTCCTCTAAGAGGAGGAAGCGCCACCCATGGCACACAGTGTCCCGTCGGACAGCAGAACCAGCCGTCGTCCCACGACACGACCCCATGC
CGCCCGCAGGGCGCCCCGGGGCTCGCGTCGGCCCGGCCGTACGCCAAAATGGCGGCTCCCGCGTATTTCCGCTCGCGCGCCGTATCGTCTTCGCCGCCTG
CGCCGGCACACCTATTGGCCCCCGCGGCGTCCCGTCGCCGCGTCGCGTTGCTGGCCCGTCGGAGCGACGCCGCTCGGGTCAGTCGGCGGCCGGACTGGGA
AGATGGACGCAGCTACTCTGACCTACGACACTCTCCGGTTTGCTGAGTTTGAAGATTTTCCTGAGACCTCAGAGCCCGTTTGGATACTGGGTAGAAAATA
CAGCATTTTCACAGAAAAGGACGAGATCTTGTCTGATGTGGCATCTAGACTTTGGTTTACATACAGGAAAAACTTTCCAGCCATTGGGGGGACAGGCCCC
ACCTCGGACACAGGCTGGGGCTGCATGCTGCGGTGTGGACAGATGATCTTTGCCCAAGCCCTGGTGTGCCGGCACCTAGGCCGAGATTGGAGGTGGACAC
AAAGGAAGAGGCAGCCAGACAGCTACTTCAGCGTCCTCAACGCATTCATCGACAGGAAGGACAGTTACTACTCCATTCACCAGATAGCGCAAATGGGAGT
TGGCGAAGGCAAGTCCATAGGCCAGTGGTACGGGCCCAACACTGTCGCCCAGGTCCTGAAGAAGCTTGCTGTCTTCGATACGTGGAGCTCCTTGGCGGTC
CACATTGCAATGGACAACACTGTTGTGATGGAGGAAATCAGAAGGTTGTGCAGGACCAGCGTTCCCTGTGCAGGCGCCACTGCGTTTCCTGCAGATTCCG
ACCGGCACTGCAACGGATTCCCTGCCGGAGCTGAGGTCACCAACAGGCCGTCGCCATGGAGACCCCTGGTACTTCTCATTCCCCTGCGCCTGGGGCTCAC
GGACATCAACGAGGCCTACGTGGAGACGCTGAAGCACTGCTTCATGATGCCCCAGTCCCTGGGCGTCATCGGAGGGAAGCCCAACAGCGCCCACTACTTC
ATCGGCTACGTTGGTGAGGAGCTCATCTACCTGGACCCCCACACCACGCAGCCAGCCGTGGAGCCCACTGATGGCTGCTTCATCCCGGACGAGAGCTTCC
ACTGCCAGCACCCGCCGTGCCGCATGAGCATCGCGGAGCTTGACCCGTCCATCGCTGTGGGGTTTTTCTGTAAGACTGAAGATGACTTCAATGATTGGTG
CCAGCAAGTCAAAAAGCTGTCTCTGCTTGGAGGTGCCCTGCCCATGTTTGAGCTGGTGGAGCTGCAGCCTTCACATCTGGCCTGCCCCGACGTCCTGAAC
CTGTCCCTAGGTGAGAGCTGCCAAGTCCAGGTGGGGTCCCTCGGAGATTCTTCTGATGTAGAGCGACTGGAAAGATTCTTCGACTCAGAAGATGAAGACT
TTGAAATCCTGTCCCTTTGAAAATCCTGGGGTCGGGGGTGGCACCTGTGAGAGCCTGGGGCTCCTGGTGCCGCTGCGTTTCATCCATCCCGCCCGCTCGC
CTGCCGAGGGCTGCGCCCCGTGCTGCCTCCCCCCAGAGGGCCACCCGCTGTGCTCGTGGACTGAGGCTGCGCTGCCCGGGAGGCCTTACTGCTTGGTGTC
AGACTGCCCAGCTCAGAGTGCCCGTCAGGGCCTGTGCATCCGCACGCGGAGCCGTCTGTTAGGAGCTTCCAGAGTGTTCTCTCGACACTGCCAGCCCCGT
GTTAGCACCTGGGCCTCAGTCCCACTTGCTCCCAGGCGCCGGTTCTGTGGTTGGTTTGGAATTAAAGTCCTGTTTGAAGTTGTCAGACACAGACATGAAT
TTCTGGGCGCTCCCTGAGTCAGAGTCTCAGAAGACCTGTGCAGGCTGGCGTGAGAGGAGCGGCAGCCACACTGCGGCCCCACGCCCAAGGACTGGGCTGC
TCTCGAGGGGGGCGCGCCCACCGCTGTGTCCTCTCTGCCCAGCCTGGCTTACCAAGGGCTACCTCAGTGGGAGATGAGGTTGGAGGAACGAAGGCGAGGT
TCCTCCTTGCTTTGGGGAGAAAAGTATTCAGGAAGTGGGTGTGTGGGAAACCTGAAGATGGCGTGCACAGGACACAGCGTGGGCGGCCTGGGCAGAAGGG
CGGCTGGCTGTCCTGGAGCTGCTGCTGGAGCCTGCCCTCAGAGTGTCCCTTTCCAGTGCTGTGGCATTCTGTGGCAGCTTCCCCAGGTGTGGTGACGGGG
GGGGGGCGGGGCCTCCACCTGTGACAGCCAGGCTTGAGGGTGGACGGCGTGCCTCTCCCAGGAGCCTTCCCCATGTCCTTGCCTTGCTGAGAATTGCCCT
CCCATGCCGCTGAGGTGTTAGGTGGTTTAGGGCCAAAAGGGGAAAACCACTTGAGTCTTGTGGTGTGTGGTGGGCAGACACCACAGGGTGGCATCACCTG
GTGGCATTTCCAGAACCTCAGCCCCGATTCCAGCACCCACCACCGCCTGACCCTGTGTAACCTGCTGTCCCGGGTCCCAGAGTGCACTCTGCCCCGCTGC
TCTGCTGCCTGTCCTGGGAAAGTATCTTTGCCCCACTAGGAAATGTAAACAGGAGGGCTTGGGGAGCGTGGGCACTTTTCTCATGAGCAGCTACTGCGGC
GTTGGCAGGACTCGCTGCTGCTGCTGCTGCTTGTGTAGGTCGGGGAGCCAGAGATCCCCGAGGACGCGCGCCGGACAGTCGGCACTGACCGGCCCACCTG
GTAGCAGAGGACACCCCCAGCCCCCCAAGCATTGAAGACATAGTGTATTTCCTCGTATCCTTTCTCCCTTGGGTGTAGTTGGGGTGGGGAAGCAGGGAAG
GCTGGTGCGATCTCCATTCCTTGGGCTCCACGTCCGAGTTCATGGTGCGCCGCTGTGCTGGGAGCTGCAGTGGTAATGTGTGGGACACCTTGACCAAAGG
GGAGCTTTGTCTCGTGTGTTTTGAAAAAGGCTTAATGAAGAGAATGTTGTTCATTCTTAGTAGTATAGTTTGCAATTCTTAATGGCAAATAATAAGTTTC
AGTAGAAAACAAA
- refseq_LOC727737.F1 (forward primer)
- refseq_LOC727737.R1 (reverse primer)
- Single exon skipping
Frame 0
LASRGFGRLRTSGAPQAPRSVRRQAGRRAGESP
RRGKSVPRHGRAEHVRTARWLLGRGQSSPLPPRQ
LLLGPLRGGSATHGTQCPVGQQNQPSSHDTTPC
RPQGAPGLASARPYAKMAAPAYFRSRAVSSSPP
APAHLLAPAASRRRVALLARRSDAARVSRRPDWE
DGRSYSDLRHSPVC*V*RFS*DLRARLDTG*KI
QHFHRKGRDLV*CGI*TLVYIQEKLSSHWGDRP
HLGHRLGLHAAVWTDDLCPSPGVPAPRPRLEVDT
KEEAARQLLQRPQRIHRQEGQLLLHSPDSANGS
WRRQVHRPVVRAQHCRPGPEEACCLRYVELLGG
PHCNGQHCCDGGNQKVVQDQRSLCRRHCVSCRFR
PALQRIPCRS*GHQQAVAMETPGTSHSPAPGAH
GHQRGLRGDAEALLHDAPVPGRHRREAQQRPLL
HRLRW*GAHLPGPPHHAASRGAH*WLLHPGRELP
LPAPAVPHEHRGA*PVHRCGVFL*D*R*LQ*LV
PASQKAVSAWRCPAHV*AGGAAAFTSGLPRRPE
PVPR*ELPSPGGVPRRFF*CRATGKILRLRR*RL
*NPVPLKILGSGVAPVRAWGSWCRCVSSIPPAR
LPRAAPRAASPQRATRCARGLRLRCPGGLTAWC
QTAQLRVPVRACASARGAVC*ELPECSLDTASPV
LAPGPQSHLLPGAGSVVGLELKSCLKLSDTDMN
FWALPESESQKTCAGWRERSGSHTAAPRPRTGL
LSRGARPPLCPLCPAWLTKGYLSGR*GWRNEGEV
PPCFGEKSIQEVGVWET*RWRAQDTAWAAWAEG
RLAVLELLLEPALRVSLSSAVAFCGSFPRCGDG
GGAGPPPVTARLEGGRRASPRSLPHVLALLRIAL
PCR*GVRWFRAKRGKPLESCGVWWADTTGWHHL
VAFPEPQPRFQHPPPPDPV*PAVPGPRVHSAPL
LCCLSWESIFAPLGNVNRRAWGAWALFS*AATAA
LAGLAAAAAACVGRGARDPRGRAPDSRH*PAHL
VAEDTPSPPSIEDIVYFLVSFLPWV*LGWGSRE
GWCDLHSLGSTSEFMVRRCAGSCSGNVWDTLTKG
ELCLVCFEKGLMKRMLFILSSIVCNS*WQIISF
SRKQ
Frame 1
SPAAGSGVFGPAGRRRPLAASVGRRADGRGSRP
GGASPYRDMGAPSTSVPQDGCSDGDRARLCRLD
NCSWVL*EEEAPPMAHSVPSDSRTSRRPTTRPHA
ARRAPRGSRRPGRTPKWRLPRISARAPYRLRRL
RRHTYWPPRRPVAASRCWPVGATPLGSVGGRTG
KMDAATLTYDTLRFAEFEDFPETSEPVWILGRKY
SIFTEKDEILSDVASRLWFTYRKNFPAIGGTGP
TSDTGWGCMLRCGQMIFAQALVCRHLGRDWRWT
QRKRQPDSYFSVLNAFIDRKDSYYSIHQIAQMGV
GEGKSIGQWYGPNTVAQVLKKLAVFDTWSSLAV
HIAMDNTVVMEEIRRLCRTSVPCAGATAFPADS
DRHCNGFPAGAEVTNRPSPWRPLVLLIPLRLGLT
DINEAYVETLKHCFMMPQSLGVIGGKPNSAHYF
IGYVGEELIYLDPHTTQPAVEPTDGCFIPDESF
HCQHPPCRMSIAELDPSIAVGFFCKTEDDFNDWC
QQVKKLSLLGGALPMFELVELQPSHLACPDVLN
LSLGESCQVQVGSLGDSSDVERLERFFDSEDED
FEILSL*KSWGRGWHL*EPGAPGAAAFHPSRPLA
CRGLRPVLPPPRGPPAVLVD*GCAAREALLLGV
RLPSSECPSGPVHPHAEPSVRSFQSVLSTLPAP
C*HLGLSPTCSQAPVLWLVWN*SPV*SCQTQT*I
SGRSLSQSLRRPVQAGVRGAAATLRPHAQGLGC
SRGGRAHRCVLSAQPGLPRATSVGDEVGGTKAR
FLLALGRKVFRKWVCGKPEDGVHRTQRGRPGQKG
GWLSWSCCWSLPSECPFPVLWHSVAASPGVVTG
GGRGLHL*QPGLRVDGVPLPGAFPMSLPC*ELP
SHAAEVLGGLGPKGENHLSLVVCGGQTPQGGITW
WHFQNLSPDSSTHHRLTLCNLLSRVPECTLPRC
SAACPGKVSLPH*EM*TGGLGERGHFSHEQLLR
RWQDSLLLLLLV*VGEPEIPEDARRTVGTDRPTW
*QRTPPAPQALKT*CISSYPFSLGCSWGGEAGK
AGAISIPWAPRPSSWCAAVLGAAVVMCGTP*PK
GSFVSCVLKKA**RECCSFLVV*FAILNGK**VS
VENK
Frame 2
RQPRVRASSDQRGAAGPSQRPSAGGQTGGGVAP
AGQVRTATWARRARPYRKMAARTGTELASAAST
TAPGSSKRRKRHPWHTVSRRTAEPAVVPRHDPM
PPAGRPGARVGPAVRQNGGSRVFPLARRIVFAAC
AGTPIGPRGVPSPRRVAGPSERRRSGQSAAGLG
RWTQLL*PTTLSGLLSLKIFLRPQSPFGYWVEN
TAFSQKRTRSCLMWHLDFGLHTGKTFQPLGGQAP
PRTQAGAACCGVDR*SLPKPWCAGT*AEIGGGH
KGRGSQTATSASSTHSSTGRTVTTPFTR*RKWE
LAKASP*ASGTGPTLSPRS*RSLLSSIRGAPWRS
TLQWTTLL*WRKSEGCAGPAFPVQAPLRFLQIP
TGTATDSLPELRSPTGRRHGDPWYFSFPCAWGS
RTSTRPTWRR*STAS*CPSPWASSEGSPTAPTTS
SATLVRSSSTWTPTPRSQPWSPLMAASSRTRAS
TASTRRAA*ASRSLTRPSLWGFSVRLKMTSMIG
ASKSKSCLCLEVPCPCLSWWSCSLHIWPAPTS*T
CP*VRAAKSRWGPSEILLM*SDWKDSSTQKMKT
LKSCPFENPGVGGGTCESLGLLVPLRFIHPARS
PAEGCAPCCLPPEGHPLCSWTEAALPGRPYCLVS
DCPAQSARQGLCIRTRSRLLGASRVFSRHCQPR
VSTWASVPLAPRRRFCGWFGIKVLFEVVRHRHE
FLGAP*VRVSEDLCRLA*EERQPHCGPTPKDWAA
LEGGAPTAVSSLPSLAYQGLPQWEMRLEERRRG
SSLLWGEKYSGSGCVGNLKMACTGHSVGGLGRR
AAGCPGAAAGACPQSVPFQCCGILWQLPQVW*RG
GGGASTCDSQA*GWTACLSQEPSPCPCLAENCP
PMPLRC*VV*GQKGKTT*VLWCVVGRHHRVASP
GGISRTSAPIPAPTTA*PCVTCCPGSQSALCPAA
LLPVLGKYLCPTRKCKQEGLGSVGTFLMSSYCG
VGRTRCCCCCLCRSGSQRSPRTRAGQSALTGPP
GSRGHPQPPKH*RHSVFPRILSPLGVVGVGKQGR
LVRSPFLGLHVRVHGAPLCWELQW*CVGHLDQR
GALSRVF*KRLNEENVVHS**YSLQFLMANNKF
Q*KT
Sequence XM_001126076
AAAAAAAAAAAAAAAAATCGGCGGCCGGACTGGGAAGATGGACGCAGCTACTCTGACCTACGACACTCTCCGGTTTGCTGAGTTTGAAGATTTTCCTGAG
ACCTCAGAGCCCGTTTGGATACTGGGTAGAAAATACAGCATTTTCACAGAAAAGGACGAGATCTTGTCTGATGTGGCATCTAGACTTTGGTTTACATACA
GGAAAAACTTTCCAGCCATTGGGGGGACAGGCCCCACCTCGGACACAGGCTGGGGCTGCATGCTGCGGTGTGGACAGATGATCTTTGCCCAAGCCCTGGT
GTGCCGGCACCTAGGCCGAGATTGGAGGTGGACACAAAGGAAGAGGCAGCCAGACAGCTACTTCAGCGTCCTCAACGCATTCATCGACAGGAAGGACAGT
TACTACTCCATTCACCAGATAGCGCAAATGGGAGTTGGCGAAGGCAAGTCCATAGGCCAGTGGTACGGGCCCAACACTGTCGCCCAGGTCCTGAAGAAGC
TTGCTGTCTTCGATACGTGGAGCTCCTTGGCGGTCCACATTGCAATGGACAACACTGTTGTGATGGAGGAAATCAGAAGGTTGTGCAGGACCAGCGTTCC
CTGTGCAGGCGCCACTGCGTTTCCTGCAGATTCCGACCGGCACTGCAACGGATTCCCTGCCGGAGCTGAGGTCACCAACAGGCCGTCGCCATGGAGACCC
CTGGTACTTCTCATTCCCCTGCGCCTGGGGCTCACGGACATCAACGAGGCCTACGTGGAGACGCTGAAGCACTGCTTCATGATGCCCCAGTCCCTGGGCG
TCATCGGAGGGAAGCCCAACAGCGCCCACTACTTCATCGGCTACGTTGGTGAGGAGCTCATCTACCTGGACCCCCACACCACGCAGCCAGCCGTGGAGCC
CACTGATGGCTGCTTCATCCCGGACGAGAGCTTCCACTGCCAGCACCCGCCGTGCCGCATGAGCATCGCGGAGCTTGACCCGTCCATCGCTGTGGGGTTT
TTCTGTAAGACTGAAGATGACTTCAATGATTGGTGCCAGCAAGTCAAAAAGCTGTCTCTGCTTGGAGGTGCCCTGCCCATGTTTGAGCTGGTGGAGCTGC
AGCCTTCACATCTGGCCTGCCCCGACGTCCTGAACCTGTCCCTAGGTGAGAGCTGCCAAGTCCAGATTCTTCTGATGTAGAGCGACTGGAAAGATTCTTC
GACTCAGAAGATGAAGACTTTGAAATCCTGTCCCTTTGAAAATCCTGGGGTCGGGGGTGGCACCTGTGAGAGCCTGGGGCTCCTGGTGCCGCTGCGTTTC
ATCCATCCCGCCCGCTCGCCTGCCGAGGGCTGCGCCCCGTGCTGCCTCCCCCCAGAGGGCCACCCGCTGTGCTCGTGGACTGAGGCTGCGCTGCCCGGGA
GGCCTTACTGCTTGGTGTCAGACTGCCCAGCTCAGAGTGCCCGTCAGGGCCTGTGCATCCGCACGCGGAGCCGTCTGTTAGGAGCTTCCAGAGTGTTCTC
TCGACACTGCCAGCCCCGTGTTAGCACCTGGGCCTCAGTCCCACTTGCTCCCAGGCGCCGGTTCTGTGGTTGGTTTGGAATTAAAGTCCTGTTTGAAGTT
GTCAGACACAGACATGAATTTCTGGGCGCTCCCTGAGTCAGAGTCTCAGAAGACCTGTGCAGGCTGGCGTGAGAGGAGCGGCAGCCACACTGCGGCCCCA
CGCCCAAGGACTGGGCTGCTCTCGAGGGGGGCGCGCCCACCGCTGTGTCCTCTCTGCCCAGCCTGGCTTACCAAGGGCTACCTCAGTGGGAGATGAGGTT
GGAGGAACGAAGGCGAGGTTCCTCCTTGCTTTGGGGAGAAAAGTATTCAGGAAGTGGGTGTGTGGGAAACCTGAAGATGGCGTGCACAGGACACAGCGTG
GGCGGCCTGGGCAGAAGGGCGGCTGGCTGTCCTGGAGCTGCTGCTGGAGCCTGCCCTCAGAGTGTCCCTTTCCAGTGCTGTGGCATTCTGTGGCAGCTTC
CCCAGGTGTGGTGACGGGGGGGGGGCGGGGCCTCCACCTGTGACAGCCAGGCTTGAGGGTGGACGGCGTGCCTCTCCCAGGAGCCTTCCCCATGTCCTTG
CCTTGCTGAGAATTGCCCTCCCATGCCGCTGAGGTGTTAGGTGGTTTAGGGCCAAAAGGGGAAAACCACTTGAGTCTTGTGGTGTGTGGTGGGCAGACAC
CACAGGGTGGCATCACCTGGTGGCATTTCCAGAACCTCAGCCCCGATTCCAGCACCCACCACCGCCTGACCCTGTGTAACCTGCTGTCCCGGGTCCCAGA
GTGCACTCTGCCCCGCTGCTCTGCTGCCTGTCCTGGGAAAGTATCTTTGCCCCACTAGGAAATGTAAACAGGAGGGCTTGGGGAGCGTGGGCACTTTTCT
CATGAGCAGCTACTGCGGCGTTGGCAGGACTCGCTGCTGCTGCTGCTGCTTGTGTAGGTCGGGGAGCCAGAGATCCCCGAGGACGCGCGCCGGACAGTCG
GCACTGACCGGCCCACCTGGTAGCAGAGGACACCCCCAGCCCCCCAAGCATTGAAGACATAGTGTATTTCCTCGTATCCTTTCTCCCTTGGGTGTAGTTG
GGGTGGGGAAGCAGGGAAGGCTGGTGCGATCTCCATTCCTTGGGCTCCACGTCCGAGTTCATGGTGCGCCGCTGTGCTGGGAGCTGCAGTGGTAATGTGT
GGGACACCTTGACCAAAGGGGAGCTTTGTCTCGTGTGTTTTGAAAAAGGCTTAATGAAGAGAATGTTGTTCATTCTTAGTAGTATAGTTTGCAATTCTTA
ATGGCAAATAATAAGTTTCAGTAGAAAACAAA
- refseq_LOC727737.F1 (forward primer)
- refseq_LOC727737.R1 (reverse primer)
- Single exon skipping
Frame 0
KKKKKNRRPDWEDGRSYSDLRHSPVC*V*RFS*
DLRARLDTG*KIQHFHRKGRDLV*CGI*TLVYIQ
EKLSSHWGDRPHLGHRLGLHAAVWTDDLCPSPG
VPAPRPRLEVDTKEEAARQLLQRPQRIHRQEGQ
LLLHSPDSANGSWRRQVHRPVVRAQHCRPGPEEA
CCLRYVELLGGPHCNGQHCCDGGNQKVVQDQRS
LCRRHCVSCRFRPALQRIPCRS*GHQQAVAMET
PGTSHSPAPGAHGHQRGLRGDAEALLHDAPVPGR
HRREAQQRPLLHRLRW*GAHLPGPPHHAASRGA
H*WLLHPGRELPLPAPAVPHEHRGA*PVHRCGV
FL*D*R*LQ*LVPASQKAVSAWRCPAHV*AGGAA
AFTSGLPRRPEPVPR*ELPSPDSSDVERLERFF
DSEDEDFEILSL*KSWGRGWHL*EPGAPGAAAF
HPSRPLACRGLRPVLPPPRGPPAVLVD*GCAARE
ALLLGVRLPSSECPSGPVHPHAEPSVRSFQSVL
STLPAPC*HLGLSPTCSQAPVLWLVWN*SPV*S
CQTQT*ISGRSLSQSLRRPVQAGVRGAAATLRPH
AQGLGCSRGGRAHRCVLSAQPGLPRATSVGDEV
GGTKARFLLALGRKVFRKWVCGKPEDGVHRTQR
GRPGQKGGWLSWSCCWSLPSECPFPVLWHSVAAS
PGVVTGGGRGLHL*QPGLRVDGVPLPGAFPMSL
PC*ELPSHAAEVLGGLGPKGENHLSLVVCGGQT
PQGGITWWHFQNLSPDSSTHHRLTLCNLLSRVPE
CTLPRCSAACPGKVSLPH*EM*TGGLGERGHFS
HEQLLRRWQDSLLLLLLV*VGEPEIPEDARRTV
GTDRPTW*QRTPPAPQALKT*CISSYPFSLGCSW
GGEAGKAGAISIPWAPRPSSWCAAVLGAAVVMC
GTP*PKGSFVSCVLKKA**RECCSFLVV*FAIL
NGK**VSVENK
Frame 1
KKKKKIGGRTGKMDAATLTYDTLRFAEFEDFPE
TSEPVWILGRKYSIFTEKDEILSDVASRLWFTY
RKNFPAIGGTGPTSDTGWGCMLRCGQMIFAQALV
CRHLGRDWRWTQRKRQPDSYFSVLNAFIDRKDS
YYSIHQIAQMGVGEGKSIGQWYGPNTVAQVLKK
LAVFDTWSSLAVHIAMDNTVVMEEIRRLCRTSVP
CAGATAFPADSDRHCNGFPAGAEVTNRPSPWRP
LVLLIPLRLGLTDINEAYVETLKHCFMMPQSLG
VIGGKPNSAHYFIGYVGEELIYLDPHTTQPAVEP
TDGCFIPDESFHCQHPPCRMSIAELDPSIAVGF
FCKTEDDFNDWCQQVKKLSLLGGALPMFELVEL
QPSHLACPDVLNLSLGESCQVQILLM*SDWKDSS
TQKMKTLKSCPFENPGVGGGTCESLGLLVPLRF
IHPARSPAEGCAPCCLPPEGHPLCSWTEAALPG
RPYCLVSDCPAQSARQGLCIRTRSRLLGASRVFS
RHCQPRVSTWASVPLAPRRRFCGWFGIKVLFEV
VRHRHEFLGAP*VRVSEDLCRLA*EERQPHCGP
TPKDWAALEGGAPTAVSSLPSLAYQGLPQWEMRL
EERRRGSSLLWGEKYSGSGCVGNLKMACTGHSV
GGLGRRAAGCPGAAAGACPQSVPFQCCGILWQL
PQVW*RGGGGASTCDSQA*GWTACLSQEPSPCPC
LAENCPPMPLRC*VV*GQKGKTT*VLWCVVGRH
HRVASPGGISRTSAPIPAPTTA*PCVTCCPGSQ
SALCPAALLPVLGKYLCPTRKCKQEGLGSVGTFL
MSSYCGVGRTRCCCCCLCRSGSQRSPRTRAGQS
ALTGPPGSRGHPQPPKH*RHSVFPRILSPLGVV
GVGKQGRLVRSPFLGLHVRVHGAPLCWELQW*CV
GHLDQRGALSRVF*KRLNEENVVHS**YSLQFL
MANNKFQ*KT
Frame 2
KKKKKSAAGLGRWTQLL*PTTLSGLLSLKIFLR
PQSPFGYWVENTAFSQKRTRSCLMWHLDFGLHT
GKTFQPLGGQAPPRTQAGAACCGVDR*SLPKPW
CAGT*AEIGGGHKGRGSQTATSASSTHSSTGRTV
TTPFTR*RKWELAKASP*ASGTGPTLSPRS*RS
LLSSIRGAPWRSTLQWTTLL*WRKSEGCAGPAF
PVQAPLRFLQIPTGTATDSLPELRSPTGRRHGDP
WYFSFPCAWGSRTSTRPTWRR*STAS*CPSPWA
SSEGSPTAPTTSSATLVRSSSTWTPTPRSQPWS
PLMAASSRTRASTASTRRAA*ASRSLTRPSLWGF
SVRLKMTSMIGASKSKSCLCLEVPCPCLSWWSC
SLHIWPAPTS*TCP*VRAAKSRFF*CRATGKIL
RLRR*RL*NPVPLKILGSGVAPVRAWGSWCRCVS
SIPPARLPRAAPRAASPQRATRCARGLRLRCPG
GLTAWCQTAQLRVPVRACASARGAVC*ELPECS
LDTASPVLAPGPQSHLLPGAGSVVGLELKSCLKL
SDTDMNFWALPESESQKTCAGWRERSGSHTAAP
RPRTGLLSRGARPPLCPLCPAWLTKGYLSGR*G
WRNEGEVPPCFGEKSIQEVGVWET*RWRAQDTAW
AAWAEGRLAVLELLLEPALRVSLSSAVAFCGSF
PRCGDGGGAGPPPVTARLEGGRRASPRSLPHVL
ALLRIALPCR*GVRWFRAKRGKPLESCGVWWADT
TGWHHLVAFPEPQPRFQHPPPPDPV*PAVPGPR
VHSAPLLCCLSWESIFAPLGNVNRRAWGAWALF
S*AATAALAGLAAAAAACVGRGARDPRGRAPDSR
H*PAHLVAEDTPSPPSIEDIVYFLVSFLPWV*L
GWGSREGWCDLHSLGSTSEFMVRRCAGSCSGNV
WDTLTKGELCLVCFEKGLMKRMLFILSSIVCNS*
WQIISFSRKQ
Sequence XM_001126139
CTCGCCAGCCGCGGGTTCGGGCGTCTTCGGACCAGCGGGGCGCCGCAGGCCCCTCGCAGCGTCCGTCGGCAGGCGGGCAGACGGGCGGGGGAGTCGCCCC
GGCGGGGCAAGTCCGTACCGCGACATGGGCGCGCCGAGCACGTCCGTACCGCAAGATGGCTGCTCGGACGGGGACAGAGCTCGCCTCTGCCGCCTCGACA
ACTGCTCCTGGGTCCTCTAAGAGGAGGAAGCGCCACCCATGGCACACAGTGTCCCGTCGGACAGCAGAACCAGCCGTCGTCCCACGACACGACCCCATGC
CGCCCGCAGGGCGCCCCGGGGCTCGCGTCGGCCCGGCCGTACGCCAAAATGGCGGCTCCCGCGTATTTCCGCTCGCGCGCCGTATCGTCTTCGCCGCCTG
CGCCGGCACACCTATTGGCCCCCGCGGCGTCCCGTCGCCGCGTCGCGTTGCTGGCCCGTCGGAGCGACGCCGCTCGGGTCAGTCGGCGGCCGGACTGGGA
AGATGGACGCAGCTACTCTGACCTACGACACTCTCCGGTTTGCTGAGTTTGAAGATTTTCCTGAGACCTCAGAGCCCGTTTGGATACTGGGTAGAAAATA
CAGCATTTTCACAGAAAAGGACGAGATCTTGTCTGATGTGGCATCTAGACTTTGGTTTACATACAGGAAAAACTTTCCAGCCATTGGGGGGACAGGCCCC
ACCTCGGACACAGGCTGGGGCTGCATGCTGCGGTGTGGACAGATGATCTTTGCCCAAGCCCTGGTGTGCCGGCACCTAGGCCGAGATTGGAGGTGGACAC
AAAGGAAGAGGCAGCCAGACAGCTACTTCAGCGTCCTCAACGCATTCATCGACAGGAAGGACAGTTACTACTCCATTCACCAGATAGCGCAAATGGGAGT
TGGCGAAGGCAAGTCCATAGGCCAGTGGTACGGGCCCAACACTGTCGCCCAGGTCCTGAAGAAGCTTGCTGTCTTCGATACGTGGAGCTCCTTGGCGGTC
CACATTGCAATGGACAACACTGTTGTGATGGAGGAAATCAGAAGGTTGTGCAGGACCAGCGTTCCCTGTGCAGGCGCCACTGCGTTTCCTGCAGATTCCG
ACCGGCACTGCAACGGATTCCCTGCCGGAGCTGAGGTCACCAACAGGCCGTCGCCATGGAGACCCCTGGTACTTCTCATTCCCCTGCGCCTGGGGCTCAC
GGACATCAACGAGGCCTACGTGGAGACGCTGAAGCACTGCTTCATGATGCCCCAGTCCCTGGGCGTCATCGGAGGGAAGCCCAACAGCGCCCACTACTTC
ATCGGCTACGTTGGTGAGGAGCTCATCTACCTGGACCCCCACACCACGCAGCCAGCCGTGGAGCCCACTGATGGCTGCTTCATCCCGGACGAGAGCTTCC
ACTGCCAGCACCCGCCGTGCCGCATGAGCATCGCGGAGCTTGACCCGTCCATCGCTGTGGGGTTTTTCTGTAAGACTGAAGATGACTTCAATGATTGGTG
CCAGCAAGTCAAAAAGCTGTCTCTGCTTGGAGGTGCCCTGCCCATGTTTGAGCTGGTGGAGCTGCAGCCTTCACATCTGGCCTGCCCCGACGTCCTGAAC
CTGTCCCTAGATTCTTCTGATGTAGAGCGACTGGAAAGATTCTTCGACTCAGAAGATGAAGACTTTGAAATCCTGTCCCTTTGAAAATCCTGGGGTCGGG
GGTGGCACCTGTGAGAGCCTGGGGCTCCTGGTGCCGCTGCGTTTCATCCATCCCGCCCGCTCGCCTGCCGAGGGCTGCGCCCCGTGCTGCCTCCCCCCAG
AGGGCCACCCGCTGTGCTCGTGGACTGAGGCTGCGCTGCCCGGGAGGCCTTACTGCTTGGTGTCAGACTGCCCAGCTCAGAGTGCCCGTCAGGGCCTGTG
CATCCGCACGCGGAGCCGTCTGTTAGGAGCTTCCAGAGTGTTCTCTCGACACTGCCAGCCCCGTGTTAGCACCTGGGCCTCAGTCCCACTTGCTCCCAGG
CGCCGGTTCTGTGGTTGGTTTGGAATTAAAGTCCTGTTTGAAGTTGTCAGACACAGACATGAATTTCTGGGCGCTCCCTGAGTCAGAGTCTCAGAAGACC
TGTGCAGGCTGGCGTGAGAGGAGCGGCAGCCACACTGCGGCCCCACGCCCAAGGACTGGGCTGCTCTCGAGGGGGGCGCGCCCACCGCTGTGTCCTCTCT
GCCCAGCCTGGCTTACCAAGGGCTACCTCAGTGGGAGATGAGGTTGGAGGAACGAAGGCGAGGTTCCTCCTTGCTTTGGGGAGAAAAGTATTCAGGAAGT
GGGTGTGTGGGAAACCTGAAGATGGCGTGCACAGGACACAGCGTGGGCGGCCTGGGCAGAAGGGCGGCTGGCTGTCCTGGAGCTGCTGCTGGAGCCTGCC
CTCAGAGTGTCCCTTTCCAGTGCTGTGGCATTCTGTGGCAGCTTCCCCAGGTGTGGTGACGGGGGGGGGGCGGGGCCTCCACCTGTGACAGCCAGGCTTG
AGGGTGGACGGCGTGCCTCTCCCAGGAGCCTTCCCCATGTCCTTGCCTTGCTGAGAATTGCCCTCCCATGCCGCTGAGGTGTTAGGTGGTTTAGGGCCAA
AAGGGGAAAACCACTTGAGTCTTGTGGTGTGTGGTGGGCAGACACCACAGGGTGGCATCACCTGGTGGCATTTCCAGAACCTCAGCCCCGATTCCAGCAC
CCACCACCGCCTGACCCTGTGTAACCTGCTGTCCCGGGTCCCAGAGTGCACTCTGCCCCGCTGCTCTGCTGCCTGTCCTGGGAAAGTATCTTTGCCCCAC
TAGGAAATGTAAACAGGAGGGCTTGGGGAGCGTGGGCACTTTTCTCATGAGCAGCTACTGCGGCGTTGGCAGGACTCGCTGCTGCTGCTGCTGCTTGTGT
AGGTCGGGGAGCCAGAGATCCCCGAGGACGCGCGCCGGACAGTCGGCACTGACCGGCCCACCTGGTAGCAGAGGACACCCCCAGCCCCCCAAGCATTGAA
GACATAGTGTATTTCCTCGTATCCTTTCTCCCTTGGGTGTAGTTGGGGTGGGGAAGCAGGGAAGGCTGGTGCGATCTCCATTCCTTGGGCTCCACGTCCG
AGTTCATGGTGCGCCGCTGTGCTGGGAGCTGCAGTGGTAATGTGTGGGACACCTTGACCAAAGGGGAGCTTTGTCTCGTGTGTTTTGAAAAAGGCTTAAT
GAAGAGAATGTTGTTCATTCTTAGTAGTATAGTTTGCAATTCTTAATGGCAAATAATAAGTTTCAGTAGAAAACAAA
- refseq_LOC727737.F1 (forward primer)
- refseq_LOC727737.R1 (reverse primer)
- Single exon skipping
Frame 0
LASRGFGRLRTSGAPQAPRSVRRQAGRRAGESP
RRGKSVPRHGRAEHVRTARWLLGRGQSSPLPPRQ
LLLGPLRGGSATHGTQCPVGQQNQPSSHDTTPC
RPQGAPGLASARPYAKMAAPAYFRSRAVSSSPP
APAHLLAPAASRRRVALLARRSDAARVSRRPDWE
DGRSYSDLRHSPVC*V*RFS*DLRARLDTG*KI
QHFHRKGRDLV*CGI*TLVYIQEKLSSHWGDRP
HLGHRLGLHAAVWTDDLCPSPGVPAPRPRLEVDT
KEEAARQLLQRPQRIHRQEGQLLLHSPDSANGS
WRRQVHRPVVRAQHCRPGPEEACCLRYVELLGG
PHCNGQHCCDGGNQKVVQDQRSLCRRHCVSCRFR
PALQRIPCRS*GHQQAVAMETPGTSHSPAPGAH
GHQRGLRGDAEALLHDAPVPGRHRREAQQRPLL
HRLRW*GAHLPGPPHHAASRGAH*WLLHPGRELP
LPAPAVPHEHRGA*PVHRCGVFL*D*R*LQ*LV
PASQKAVSAWRCPAHV*AGGAAAFTSGLPRRPE
PVPRFF*CRATGKILRLRR*RL*NPVPLKILGSG
VAPVRAWGSWCRCVSSIPPARLPRAAPRAASPQ
RATRCARGLRLRCPGGLTAWCQTAQLRVPVRAC
ASARGAVC*ELPECSLDTASPVLAPGPQSHLLPG
AGSVVGLELKSCLKLSDTDMNFWALPESESQKT
CAGWRERSGSHTAAPRPRTGLLSRGARPPLCPL
CPAWLTKGYLSGR*GWRNEGEVPPCFGEKSIQEV
GVWET*RWRAQDTAWAAWAEGRLAVLELLLEPA
LRVSLSSAVAFCGSFPRCGDGGGAGPPPVTARL
EGGRRASPRSLPHVLALLRIALPCR*GVRWFRAK
RGKPLESCGVWWADTTGWHHLVAFPEPQPRFQH
PPPPDPV*PAVPGPRVHSAPLLCCLSWESIFAP
LGNVNRRAWGAWALFS*AATAALAGLAAAAAACV
GRGARDPRGRAPDSRH*PAHLVAEDTPSPPSIE
DIVYFLVSFLPWV*LGWGSREGWCDLHSLGSTS
EFMVRRCAGSCSGNVWDTLTKGELCLVCFEKGLM
KRMLFILSSIVCNS*WQIISFSRKQ
Frame 1
SPAAGSGVFGPAGRRRPLAASVGRRADGRGSRP
GGASPYRDMGAPSTSVPQDGCSDGDRARLCRLD
NCSWVL*EEEAPPMAHSVPSDSRTSRRPTTRPHA
ARRAPRGSRRPGRTPKWRLPRISARAPYRLRRL
RRHTYWPPRRPVAASRCWPVGATPLGSVGGRTG
KMDAATLTYDTLRFAEFEDFPETSEPVWILGRKY
SIFTEKDEILSDVASRLWFTYRKNFPAIGGTGP
TSDTGWGCMLRCGQMIFAQALVCRHLGRDWRWT
QRKRQPDSYFSVLNAFIDRKDSYYSIHQIAQMGV
GEGKSIGQWYGPNTVAQVLKKLAVFDTWSSLAV
HIAMDNTVVMEEIRRLCRTSVPCAGATAFPADS
DRHCNGFPAGAEVTNRPSPWRPLVLLIPLRLGLT
DINEAYVETLKHCFMMPQSLGVIGGKPNSAHYF
IGYVGEELIYLDPHTTQPAVEPTDGCFIPDESF
HCQHPPCRMSIAELDPSIAVGFFCKTEDDFNDWC
QQVKKLSLLGGALPMFELVELQPSHLACPDVLN
LSLDSSDVERLERFFDSEDEDFEILSL*KSWGR
GWHL*EPGAPGAAAFHPSRPLACRGLRPVLPPPR
GPPAVLVD*GCAAREALLLGVRLPSSECPSGPV
HPHAEPSVRSFQSVLSTLPAPC*HLGLSPTCSQ
APVLWLVWN*SPV*SCQTQT*ISGRSLSQSLRRP
VQAGVRGAAATLRPHAQGLGCSRGGRAHRCVLS
AQPGLPRATSVGDEVGGTKARFLLALGRKVFRK
WVCGKPEDGVHRTQRGRPGQKGGWLSWSCCWSLP
SECPFPVLWHSVAASPGVVTGGGRGLHL*QPGL
RVDGVPLPGAFPMSLPC*ELPSHAAEVLGGLGP
KGENHLSLVVCGGQTPQGGITWWHFQNLSPDSST
HHRLTLCNLLSRVPECTLPRCSAACPGKVSLPH
*EM*TGGLGERGHFSHEQLLRRWQDSLLLLLLV
*VGEPEIPEDARRTVGTDRPTW*QRTPPAPQALK
T*CISSYPFSLGCSWGGEAGKAGAISIPWAPRP
SSWCAAVLGAAVVMCGTP*PKGSFVSCVLKKA*
*RECCSFLVV*FAILNGK**VSVENK
Frame 2
RQPRVRASSDQRGAAGPSQRPSAGGQTGGGVAP
AGQVRTATWARRARPYRKMAARTGTELASAAST
TAPGSSKRRKRHPWHTVSRRTAEPAVVPRHDPM
PPAGRPGARVGPAVRQNGGSRVFPLARRIVFAAC
AGTPIGPRGVPSPRRVAGPSERRRSGQSAAGLG
RWTQLL*PTTLSGLLSLKIFLRPQSPFGYWVEN
TAFSQKRTRSCLMWHLDFGLHTGKTFQPLGGQAP
PRTQAGAACCGVDR*SLPKPWCAGT*AEIGGGH
KGRGSQTATSASSTHSSTGRTVTTPFTR*RKWE
LAKASP*ASGTGPTLSPRS*RSLLSSIRGAPWRS
TLQWTTLL*WRKSEGCAGPAFPVQAPLRFLQIP
TGTATDSLPELRSPTGRRHGDPWYFSFPCAWGS
RTSTRPTWRR*STAS*CPSPWASSEGSPTAPTTS
SATLVRSSSTWTPTPRSQPWSPLMAASSRTRAS
TASTRRAA*ASRSLTRPSLWGFSVRLKMTSMIG
ASKSKSCLCLEVPCPCLSWWSCSLHIWPAPTS*T
CP*ILLM*SDWKDSSTQKMKTLKSCPFENPGVG
GGTCESLGLLVPLRFIHPARSPAEGCAPCCLPP
EGHPLCSWTEAALPGRPYCLVSDCPAQSARQGLC
IRTRSRLLGASRVFSRHCQPRVSTWASVPLAPR
RRFCGWFGIKVLFEVVRHRHEFLGAP*VRVSED
LCRLA*EERQPHCGPTPKDWAALEGGAPTAVSSL
PSLAYQGLPQWEMRLEERRRGSSLLWGEKYSGS
GCVGNLKMACTGHSVGGLGRRAAGCPGAAAGAC
PQSVPFQCCGILWQLPQVW*RGGGGASTCDSQA*
GWTACLSQEPSPCPCLAENCPPMPLRC*VV*GQ
KGKTT*VLWCVVGRHHRVASPGGISRTSAPIPA
PTTA*PCVTCCPGSQSALCPAALLPVLGKYLCPT
RKCKQEGLGSVGTFLMSSYCGVGRTRCCCCCLC
RSGSQRSPRTRAGQSALTGPPGSRGHPQPPKH*
RHSVFPRILSPLGVVGVGKQGRLVRSPFLGLHVR
VHGAPLCWELQW*CVGHLDQRGALSRVF*KRLN
EENVVHS**YSLQFLMANNKFQ*KT
Sequence XM_001126099
CTCGCCAGCCGCGGGTTCGGGCGTCTTCGGACCAGCGGGGCGCCGCAGGCCCCTCGCAGCGTCCGTCGGCAGGCGGGCAGACGGGCGGGGGAGTCGCCCC
GGCGGGGCAAGTCCGTACCGCGACATGGGCGCGCCGAGCACGTCCGTACCGCAAGATGGCTGCTCGGACGGGGACAGAGCTCGCCTCTGCCGCCTCGACA
ACTGCTCCTGGGTCCTCTAAGAGGAGGAAGCGCCACCCATGGCACACAGTGTCCCGTCGGACAGCAGAACCAGCCGTCGTCCCACGACACGACCCCATGC
CGCCCGCAGGGCGCCCCGGGGCTCGCGTCGGCCCGGCCGTACGCCAAAATGGCGGCTCCCGCGTATTTCCGCTCGCGCGCCGTATCGTCTTCGCCGCCTG
CGCCGGCACACCTATTGGCCCCCGCGGCGTCCCGTCGCCGCGTCGCGTTGCTGGCCCGTCGGAGCGACGCCGCTCGGGTCAGTCGGCGGCCGGACTGGGA
AGATGGACGCAGCTACTCTGACCTACGACACTCTCCGGTTTGCTGAGTTTGAAGATTTTCCTGAGACCTCAGAGCCCGTTTGGATACTGGGTAGAAAATA
CAGCATTTTCACAGAAAAGGACGAGATCTTGTCTGATGTGGCATCTAGACTTTGGTTTACATACAGGAAAAACTTTCCAGCCATTGGGGGGACAGGCCCC
ACCTCGGACACAGGCTGGGGCTGCATGCTGCGGTGTGGACAGATGATCTTTGCCCAAGCCCTGGTGTGCCGGCACCTAGGCCGAGATTGGAGGTGGACAC
AAAGGAAGAGGCAGCCAGACAGCTACTTCAGCGTCCTCAACGCATTCATCGACAGGAAGGACAGTTACTACTCCATTCACCAGATAGCGCAAATGGGAGT
TGGCGAAGGCAAGTCCATAGGCCAGTGGTACGGGCCCAACACTGTCGCCCAGGTCCTGAAGAAGCTTGCTGTCTTCGATACGTGGAGCTCCTTGGCGGTC
CACATTGCAATGGACAACACTGTTGTGATGGAGGAAATCAGAAGGTTGTGCAGGACCAGCGTTCCCTGTGCAGGCGCCACTGCGTTTCCTGCAGATTCCG
ACCGGCACTGCAACGGATTCCCTGCCGGAGCTGAGGTCACCAACAGGCCGTCGCCATGGAGACCCCTGGTACTTCTCATTCCCCTGCGCCTGGGGCTCAC
GGACATCAACGAGGCCTACGTGGAGACGCTGAAGCACTGCTTCATGATGCCCCAGTCCCTGGGCGTCATCGGAGGGAAGCCCAACAGCGCCCACTACTTC
ATCGGCTACGTTGGTGAGGAGCTCATCTACCTGGACCCCCACACCACGCAGCCAGCCGTGGAGCCCACTGATGGCTGCTTCATCCCGGACGAGAGCTTCC
ACTGCCAGCACCCGCCGTGCCGCATGAGCATCGCGGAGCTTGACCCGTCCATCGCTGTGGGGTTTTTCTGTAAGACTGAAGATGACTTCAATGATTGGTG
CCAGCAAGTCAAAAAGCTGTCTCTGCTTGGAGGTGCCCTGCCCATGTTTGAGCTGGTGGAGCTGCAGCCTTCACATCTGGCCTGCCCCGACGTCCTGAAC
CTGTCCCTAGGTGAGAGCTGCCAAGTCCAGATTCTTCTGATGTAGAGCGACTGGAAAGATTCTTCGACTCAGAAGATGAAGACTTTGAAATCCTGTCCCT
TTGAAAATCCTGGGGTCGGGGGTGGCACCTGTGAGAGCCTGGGGCTCCTGGTGCCGCTGCGTTTCATCCATCCCGCCCGCTCGCCTGCCGAGGGCTGCGC
CCCGTGCTGCCTCCCCCCAGAGGGCCACCCGCTGTGCTCGTGGACTGAGGCTGCGCTGCCCGGGAGGCCTTACTGCTTGGTGTCAGACTGCCCAGCTCAG
AGTGCCCGTCAGGGCCTGTGCATCCGCACGCGGAGCCGTCTGTTAGGAGCTTCCAGAGTGTTCTCTCGACACTGCCAGCCCCGTGTTAGCACCTGGGCCT
CAGTCCCACTTGCTCCCAGGCGCCGGTTCTGTGGTTGGTTTGGAATTAAAGTCCTGTTTGAAGTTGTCAGACACAGACATGAATTTCTGGGCGCTCCCTG
AGTCAGAGTCTCAGAAGACCTGTGCAGGCTGGCGTGAGAGGAGCGGCAGCCACACTGCGGCCCCACGCCCAAGGACTGGGCTGCTCTCGAGGGGGGCGCG
CCCACCGCTGTGTCCTCTCTGCCCAGCCTGGCTTACCAAGGGCTACCTCAGTGGGAGATGAGGTTGGAGGAACGAAGGCGAGGTTCCTCCTTGCTTTGGG
GAGAAAAGTATTCAGGAAGTGGGTGTGTGGGAAACCTGAAGATGGCGTGCACAGGACACAGCGTGGGCGGCCTGGGCAGAAGGGCGGCTGGCTGTCCTGG
AGCTGCTGCTGGAGCCTGCCCTCAGAGTGTCCCTTTCCAGTGCTGTGGCATTCTGTGGCAGCTTCCCCAGGTGTGGTGACGGGGGGGGGGCGGGGCCTCC
ACCTGTGACAGCCAGGCTTGAGGGTGGACGGCGTGCCTCTCCCAGGAGCCTTCCCCATGTCCTTGCCTTGCTGAGAATTGCCCTCCCATGCCGCTGAGGT
GTTAGGTGGTTTAGGGCCAAAAGGGGAAAACCACTTGAGTCTTGTGGTGTGTGGTGGGCAGACACCACAGGGTGGCATCACCTGGTGGCATTTCCAGAAC
CTCAGCCCCGATTCCAGCACCCACCACCGCCTGACCCTGTGTAACCTGCTGTCCCGGGTCCCAGAGTGCACTCTGCCCCGCTGCTCTGCTGCCTGTCCTG
GGAAAGTATCTTTGCCCCACTAGGAAATGTAAACAGGAGGGCTTGGGGAGCGTGGGCACTTTTCTCATGAGCAGCTACTGCGGCGTTGGCAGGACTCGCT
GCTGCTGCTGCTGCTTGTGTAGGTCGGGGAGCCAGAGATCCCCGAGGACGCGCGCCGGACAGTCGGCACTGACCGGCCCACCTGGTAGCAGAGGACACCC
CCAGCCCCCCAAGCATTGAAGACATAGTGTATTTCCTCGTATCCTTTCTCCCTTGGGTGTAGTTGGGGTGGGGAAGCAGGGAAGGCTGGTGCGATCTCCA
TTCCTTGGGCTCCACGTCCGAGTTCATGGTGCGCCGCTGTGCTGGGAGCTGCAGTGGTAATGTGTGGGACACCTTGACCAAAGGGGAGCTTTGTCTCGTG
TGTTTTGAAAAAGGCTTAATGAAGAGAATGTTGTTCATTCTTAGTAGTATAGTTTGCAATTCTTAATGGCAAATAATAAGTTTCAGTAGAAAACAAA
- refseq_LOC727737.F1 (forward primer)
- refseq_LOC727737.R1 (reverse primer)
- Single exon skipping
Frame 0
LASRGFGRLRTSGAPQAPRSVRRQAGRRAGESP
RRGKSVPRHGRAEHVRTARWLLGRGQSSPLPPRQ
LLLGPLRGGSATHGTQCPVGQQNQPSSHDTTPC
RPQGAPGLASARPYAKMAAPAYFRSRAVSSSPP
APAHLLAPAASRRRVALLARRSDAARVSRRPDWE
DGRSYSDLRHSPVC*V*RFS*DLRARLDTG*KI
QHFHRKGRDLV*CGI*TLVYIQEKLSSHWGDRP
HLGHRLGLHAAVWTDDLCPSPGVPAPRPRLEVDT
KEEAARQLLQRPQRIHRQEGQLLLHSPDSANGS
WRRQVHRPVVRAQHCRPGPEEACCLRYVELLGG
PHCNGQHCCDGGNQKVVQDQRSLCRRHCVSCRFR
PALQRIPCRS*GHQQAVAMETPGTSHSPAPGAH
GHQRGLRGDAEALLHDAPVPGRHRREAQQRPLL
HRLRW*GAHLPGPPHHAASRGAH*WLLHPGRELP
LPAPAVPHEHRGA*PVHRCGVFL*D*R*LQ*LV
PASQKAVSAWRCPAHV*AGGAAAFTSGLPRRPE
PVPR*ELPSPDSSDVERLERFFDSEDEDFEILSL
*KSWGRGWHL*EPGAPGAAAFHPSRPLACRGLR
PVLPPPRGPPAVLVD*GCAAREALLLGVRLPSS
ECPSGPVHPHAEPSVRSFQSVLSTLPAPC*HLGL
SPTCSQAPVLWLVWN*SPV*SCQTQT*ISGRSL
SQSLRRPVQAGVRGAAATLRPHAQGLGCSRGGR
AHRCVLSAQPGLPRATSVGDEVGGTKARFLLALG
RKVFRKWVCGKPEDGVHRTQRGRPGQKGGWLSW
SCCWSLPSECPFPVLWHSVAASPGVVTGGGRGL
HL*QPGLRVDGVPLPGAFPMSLPC*ELPSHAAEV
LGGLGPKGENHLSLVVCGGQTPQGGITWWHFQN
LSPDSSTHHRLTLCNLLSRVPECTLPRCSAACP
GKVSLPH*EM*TGGLGERGHFSHEQLLRRWQDSL
LLLLLV*VGEPEIPEDARRTVGTDRPTW*QRTP
PAPQALKT*CISSYPFSLGCSWGGEAGKAGAIS
IPWAPRPSSWCAAVLGAAVVMCGTP*PKGSFVSC
VLKKA**RECCSFLVV*FAILNGK**VSVENK
Frame 1
SPAAGSGVFGPAGRRRPLAASVGRRADGRGSRP
GGASPYRDMGAPSTSVPQDGCSDGDRARLCRLD
NCSWVL*EEEAPPMAHSVPSDSRTSRRPTTRPHA
ARRAPRGSRRPGRTPKWRLPRISARAPYRLRRL
RRHTYWPPRRPVAASRCWPVGATPLGSVGGRTG
KMDAATLTYDTLRFAEFEDFPETSEPVWILGRKY
SIFTEKDEILSDVASRLWFTYRKNFPAIGGTGP
TSDTGWGCMLRCGQMIFAQALVCRHLGRDWRWT
QRKRQPDSYFSVLNAFIDRKDSYYSIHQIAQMGV
GEGKSIGQWYGPNTVAQVLKKLAVFDTWSSLAV
HIAMDNTVVMEEIRRLCRTSVPCAGATAFPADS
DRHCNGFPAGAEVTNRPSPWRPLVLLIPLRLGLT
DINEAYVETLKHCFMMPQSLGVIGGKPNSAHYF
IGYVGEELIYLDPHTTQPAVEPTDGCFIPDESF
HCQHPPCRMSIAELDPSIAVGFFCKTEDDFNDWC
QQVKKLSLLGGALPMFELVELQPSHLACPDVLN
LSLGESCQVQILLM*SDWKDSSTQKMKTLKSCP
FENPGVGGGTCESLGLLVPLRFIHPARSPAEGCA
PCCLPPEGHPLCSWTEAALPGRPYCLVSDCPAQ
SARQGLCIRTRSRLLGASRVFSRHCQPRVSTWA
SVPLAPRRRFCGWFGIKVLFEVVRHRHEFLGAP*
VRVSEDLCRLA*EERQPHCGPTPKDWAALEGGA
PTAVSSLPSLAYQGLPQWEMRLEERRRGSSLLW
GEKYSGSGCVGNLKMACTGHSVGGLGRRAAGCPG
AAAGACPQSVPFQCCGILWQLPQVW*RGGGGAS
TCDSQA*GWTACLSQEPSPCPCLAENCPPMPLR
C*VV*GQKGKTT*VLWCVVGRHHRVASPGGISRT
SAPIPAPTTA*PCVTCCPGSQSALCPAALLPVL
GKYLCPTRKCKQEGLGSVGTFLMSSYCGVGRTR
CCCCCLCRSGSQRSPRTRAGQSALTGPPGSRGHP
QPPKH*RHSVFPRILSPLGVVGVGKQGRLVRSP
FLGLHVRVHGAPLCWELQW*CVGHLDQRGALSR
VF*KRLNEENVVHS**YSLQFLMANNKFQ*KT
Frame 2
RQPRVRASSDQRGAAGPSQRPSAGGQTGGGVAP
AGQVRTATWARRARPYRKMAARTGTELASAAST
TAPGSSKRRKRHPWHTVSRRTAEPAVVPRHDPM
PPAGRPGARVGPAVRQNGGSRVFPLARRIVFAAC
AGTPIGPRGVPSPRRVAGPSERRRSGQSAAGLG
RWTQLL*PTTLSGLLSLKIFLRPQSPFGYWVEN
TAFSQKRTRSCLMWHLDFGLHTGKTFQPLGGQAP
PRTQAGAACCGVDR*SLPKPWCAGT*AEIGGGH
KGRGSQTATSASSTHSSTGRTVTTPFTR*RKWE
LAKASP*ASGTGPTLSPRS*RSLLSSIRGAPWRS
TLQWTTLL*WRKSEGCAGPAFPVQAPLRFLQIP
TGTATDSLPELRSPTGRRHGDPWYFSFPCAWGS
RTSTRPTWRR*STAS*CPSPWASSEGSPTAPTTS
SATLVRSSSTWTPTPRSQPWSPLMAASSRTRAS
TASTRRAA*ASRSLTRPSLWGFSVRLKMTSMIG
ASKSKSCLCLEVPCPCLSWWSCSLHIWPAPTS*T
CP*VRAAKSRFF*CRATGKILRLRR*RL*NPVP
LKILGSGVAPVRAWGSWCRCVSSIPPARLPRAA
PRAASPQRATRCARGLRLRCPGGLTAWCQTAQLR
VPVRACASARGAVC*ELPECSLDTASPVLAPGP
QSHLLPGAGSVVGLELKSCLKLSDTDMNFWALP
ESESQKTCAGWRERSGSHTAAPRPRTGLLSRGAR
PPLCPLCPAWLTKGYLSGR*GWRNEGEVPPCFG
EKSIQEVGVWET*RWRAQDTAWAAWAEGRLAVL
ELLLEPALRVSLSSAVAFCGSFPRCGDGGGAGPP
PVTARLEGGRRASPRSLPHVLALLRIALPCR*G
VRWFRAKRGKPLESCGVWWADTTGWHHLVAFPE
PQPRFQHPPPPDPV*PAVPGPRVHSAPLLCCLSW
ESIFAPLGNVNRRAWGAWALFS*AATAALAGLA
AAAAACVGRGARDPRGRAPDSRH*PAHLVAEDT
PSPPSIEDIVYFLVSFLPWV*LGWGSREGWCDLH
SLGSTSEFMVRRCAGSCSGNVWDTLTKGELCLV
CFEKGLMKRMLFILSSIVCNS*WQIISFSRKQ
Sequence XM_001125933
CTCGCCAGCCGCGGGTTCGGGCGTCTTCGGACCAGCGGGGCGCCGCAGGCCCCTCGCAGCGTCCGTCGGCAGGCGGGCAGACGGGCGGGGGAGTCGCCCC
GGCGGGGCAAGTCCGTACCGCGACATGGGCGCGCCGAGCACGTCCGTACCGCAAGATGGCTGCTCGGACGGGGACAGAGCTCGCCTCTGCCGCCTCGACA
ACTGCTCCTGGGTCCTCTAAGAGGAGGAAGCGCCACCCATGGCACACAGTGTCCCGTCGGACAGCAGAACCAGCCGTCGTCCCACGACACGACCCCATGC
CGCCCGCAGGGCGCCCCGGGGCTCGCGTCGGCCCGGCCGTACGCCAAAATGGCGGCTCCCGCGTATTTCCGCTCGCGCGCCGTATCGTCTTCGCCGCCTG
CGCCGGCACACCTATTGGCCCCCGCGGCGTCCCGTCGCCGCGTCGCGTTGCTGGCCCGTCGGAGCGACGCCGCTCGGGTCAGTCGGCGGCCGGACTGGGA
AGATGGACGCAGCTACTCTGACCTACGACACTCTCCGGTTTGCTGAGTTTGAAGATTTTCCTGAGACCTCAGAGCCCGTTTGGATACTGGGTAGAAAATA
CAGCATTTTCACAGAAAAGGACGAGATCTTGTCTGATGTGGCATCTAGACTTTGGTTTACATACAGGAAAAACTTTCCAGCCATTGGAGGTTGTCGGGAC
ATTTCACTCTCCAGTAAATGTGGCCCTTGGCGTTACTGAGTCCTAAGAGGGGGGACAGGCCCCACCTCGGACACAGGCTGGGGCTGCATGCTGCGGTGTG
GACAGATGATCTTTGCCCAAGCCCTGGTGTGCCGGCACCTAGGCCGAGATTGGAGGTGGACACAAAGGAAGAGGCAGCCAGACAGCTACTTCAGCGTCCT
CAACGCATTCATCGACAGGAAGGACAGTTACTACTCCATTCACCAGATAGCGCAAATGGGAGTTGGCGAAGGCAAGTCCATAGGCCAGTGGTACGGGCCC
AACACTGTCGCCCAGGTCCTGAAGAAGCTTGCTGTCTTCGATACGTGGAGCTCCTTGGCGGTCCACATTGCAATGGACAACACTGTTGTGATGGAGGAAA
TCAGAAGGTTGTGCAGGACCAGCGTTCCCTGTGCAGGCGCCACTGCGTTTCCTGCAGATTCCGACCGGCACTGCAACGGATTCCCTGCCGGAGCTGAGGT
CACCAACAGGCCGTCGCCATGGAGACCCCTGGTACTTCTCATTCCCCTGCGCCTGGGGCTCACGGACATCAACGAGGCCTACGTGGAGACGCTGAAGCAC
TGCTTCATGATGCCCCAGTCCCTGGGCGTCATCGGAGGGAAGCCCAACAGCGCCCACTACTTCATCGGCTACGTTGGTGAGGAGCTCATCTACCTGGACC
CCCACACCACGCAGCCAGCCGTGGAGCCCACTGATGGCTGCTTCATCCCGGACGAGAGCTTCCACTGCCAGCACCCGCCGTGCCGCATGAGCATCGCGGA
GCTTGACCCGTCCATCGCTGTGGGGTTTTTCTGTAAGACTGAAGATGACTTCAATGATTGGTGCCAGCAAGTCAAAAAGCTGTCTCTGCTTGGAGGTGCC
CTGCCCATGTTTGAGCTGGTGGAGCTGCAGCCTTCACATCTGGCCTGCCCCGACGTCCTGAACCTGTCCCTAGGTGAGAGCTGCCAAGTCCAGGTGGGGT
CCCTCGGAGGTACGATCTGTGCCCTTGCTTCCCCAGTCCTGGCCCCCTTGGTTTTGACCATTAAGGTGTGTGTGAGCCTGAGCCGTGAGCACTTGGCAGT
GGTTCGCCTGTGAGACCAGGTATGGAGTGGAGCGTCCCCTCCTCCAAGCTTGCGCCCAGCAGCCCAGGACCCACCTCGTCTTCCCCACCAGCGCTGCCTG
CCCGGGCGCTGTGGAGCTGGGCGTGCTACCATGGAGTCCTCAGGGGTCTGGAGCAGACAGAACATGCAGGCTCTGTGGTGACGCAGTCCTGGGTGGGGGA
CTGGTTCACTTGGGCACCACTGGCCATGGGTGGCGTAGACCCCTCGGACCATGGCCAGCGTGCCGCAGGAGCCGGCCTGGGCTCGTGCAGTGAAGTGAGT
GGCCGTGAGCGCGTCCTCCTCATCTCTGTCTCCCTGTGGGAAACTCTACAAACAAGGCAATGGCAATGGAACCACTCCTGATGACCACGAGGGTCAGACG
CGGGACAGAGGCCCCTCAGGCCTGAGATTGTGCCGGCCGCCCCCTGCCCTCCTCACCCTGCCCTGCTCCTCTTCTCTGCTCCCTCCCCCCATATTCGCAG
GTCTGCACAACCCCCGGACCTGTTCACACCCGCATGGGGACAGCTGTCTGTGGGCTGCAGAGCAGGCACTGCTCAGTCTGCCCCACGCCAAGGGCCCTTG
ACTCACACCCAGGTGGCCCACCCAAGATGCCTGATGCGCTATGTCCTGTTCCTTCTAGATTCTTCTGATGTAGAGCGACTGGAAAGATTCTTCGACTCAG
AAGATGAAGACTTTGAAATCCTGTCCCTTTGAAAATCCTGGGGTCGGGGGTGGCACCTGTGAGAGCCTGGGGCTCCTGGTGCCGCTGCGTTTCATCCATC
CCGCCCGCTCGCCTGCCGAGGGCTGCGCCCCGTGCTGCCTCCCCCCAGAGGGCCACCCGCTGTGCTCGTGGACTGAGGCTGCGCTGCCCGGGAGGCCTTA
CTGCTTGGTGTCAGACTGCCCAGCTCAGAGTGCCCGTCAGGGCCTGTGCATCCGCACGCGGAGCCGTCTGTTAGGAGCTTCCAGAGTGTTCTCTCGACAC
TGCCAGCCCCGTGTTAGCACCTGGGCCTCAGTCCCACTTGCTCCCAGGCGCCGGTTCTGTGGTTGGTTTGGAATTAAAGTCCTGTTTGAAGTTGTCAGAC
ACAGACATGAATTTCTGGGCGCTCCCTGAGTCAGAGTCTCAGAAGACCTGTGCAGGCTGGCGTGAGAGGAGCGGCAGCCACACTGCGGCCCCACGCCCAA
GGACTGGGCTGCTCTCGAGGGGGGCGCGCCCACCGCTGTGTCCTCTCTGCCCAGCCTGGCTTACCAAGGGCTACCTCAGTGGGAGATGAGGTTGGAGGAA
CGAAGGCGAGGTTCCTCCTTGCTTTGGGGAGAAAAGTATTCAGGAAGTGGGTGTGTGGGAAACCTGAAGATGGCGTGCACAGGACACAGCGTGGGCGGCC
TGGGCAGAAGGGCGGCTGGCTGTCCTGGAGCTGCTGCTGGAGCCTGCCCTCAGAGTGTCCCTTTCCAGTGCTGTGGCATTCTGTGGCAGCTTCCCCAGGT
GTGGTGACGGGGGGGGGGCGGGGCCTCCACCTGTGACAGCCAGGCTTGAGGGTGGACGGCGTGCCTCTCCCAGGAGCCTTCCCCATGTCCTTGCCTTGCT
GAGAATTGCCCTCCCATGCCGCTGAGGTGTTAGGTGGTTTAGGGCCAAAAGGGGAAAACCACTTGAGTCTTGTGGTGTGTGGTGGGCAGACACCACAGGG
TGGCATCACCTGGTGGCATTTCCAGAACCTCAGCCCCGATTCCAGCACCCACCACCGCCTGACCCTGTGTAACCTGCTGTCCCGGGTCCCAGAGTGCACT
CTGCCCCGCTGCTCTGCTGCCTGTCCTGGGAAAGTATCTTTGCCCCACTAGGAAATGTAAACAGGAGGGCTTGGGGAGCGTGGGCACTTTTCTCATGAGC
AGCTACTGCGGCGTTGGCAGGACTCGCTGCTGCTGCTGCTGCTTGTGTAGGTCGGGGAGCCAGAGATCCCCGAGGACGCGCGCCGGACAGTCGGCACTGA
CCGGCCCACCTGGTAGCAGAGGACACCCCCAGCCCCCCAAGCATTGAAGACATAGTGTATTTCCTCGTATCCTTTCTCCCTTGGGTGTAGTTGGGGTGGG
GAAGCAGGGAAGGCTGGTGCGATCTCCATTCCTTGGGCTCCACGTCCGAGTTCATGGTGCGCCGCTGTGCTGGGAGCTGCAGTGGTAATGTGTGGGACAC
CTTGACCAAAGGGGAGCTTTGTCTCGTGTGTTTTGAAAAAGGCTTAATGAAGAGAATGTTGTTCATTCTTAGTAGTATAGTTTGCAATTCTTAATGGCAA
ATAATAAGTTTCAGTAGAAAACAAA
- refseq_LOC727737.F1 (forward primer)
- refseq_LOC727737.R1 (reverse primer)
- Single exon skipping
Frame 0
LASRGFGRLRTSGAPQAPRSVRRQAGRRAGESP
RRGKSVPRHGRAEHVRTARWLLGRGQSSPLPPRQ
LLLGPLRGGSATHGTQCPVGQQNQPSSHDTTPC
RPQGAPGLASARPYAKMAAPAYFRSRAVSSSPP
APAHLLAPAASRRRVALLARRSDAARVSRRPDWE
DGRSYSDLRHSPVC*V*RFS*DLRARLDTG*KI
QHFHRKGRDLV*CGI*TLVYIQEKLSSHWRLSG
HFTLQ*MWPLALLSPKRGDRPHLGHRLGLHAAVW
TDDLCPSPGVPAPRPRLEVDTKEEAARQLLQRP
QRIHRQEGQLLLHSPDSANGSWRRQVHRPVVRA
QHCRPGPEEACCLRYVELLGGPHCNGQHCCDGGN
QKVVQDQRSLCRRHCVSCRFRPALQRIPCRS*G
HQQAVAMETPGTSHSPAPGAHGHQRGLRGDAEA
LLHDAPVPGRHRREAQQRPLLHRLRW*GAHLPGP
PHHAASRGAH*WLLHPGRELPLPAPAVPHEHRG
A*PVHRCGVFL*D*R*LQ*LVPASQKAVSAWRC
PAHV*AGGAAAFTSGLPRRPEPVPR*ELPSPGGV
PRRYDLCPCFPSPGPLGFDH*GVCEPEP*ALGS
GSPVRPGMEWSVPSSKLAPSSPGPTSSSPPALP
ARALWSWACYHGVLRGLEQTEHAGSVVTQSWVGD
WFTWAPLAMGGVDPSDHGQRAAGAGLGSCSEVS
GRERVLLISVSLWETLQTRQWQWNHS**PRGSD
AGQRPLRPEIVPAAPCPPHPALLLFSAPSPHIRR
SAQPPDLFTPAWGQLSVGCRAGTAQSAPRQGPL
THTQVAHPRCLMRYVLFLLDSSDVERLERFFDS
EDEDFEILSL*KSWGRGWHL*EPGAPGAAAFHPS
RPLACRGLRPVLPPPRGPPAVLVD*GCAAREAL
LLGVRLPSSECPSGPVHPHAEPSVRSFQSVLST
LPAPC*HLGLSPTCSQAPVLWLVWN*SPV*SCQT
QT*ISGRSLSQSLRRPVQAGVRGAAATLRPHAQ
GLGCSRGGRAHRCVLSAQPGLPRATSVGDEVGG
TKARFLLALGRKVFRKWVCGKPEDGVHRTQRGRP
GQKGGWLSWSCCWSLPSECPFPVLWHSVAASPG
VVTGGGRGLHL*QPGLRVDGVPLPGAFPMSLPC
*ELPSHAAEVLGGLGPKGENHLSLVVCGGQTPQG
GITWWHFQNLSPDSSTHHRLTLCNLLSRVPECT
LPRCSAACPGKVSLPH*EM*TGGLGERGHFSHE
QLLRRWQDSLLLLLLV*VGEPEIPEDARRTVGTD
RPTW*QRTPPAPQALKT*CISSYPFSLGCSWGG
EAGKAGAISIPWAPRPSSWCAAVLGAAVVMCGT
P*PKGSFVSCVLKKA**RECCSFLVV*FAILNGK
**VSVENK
Frame 1
SPAAGSGVFGPAGRRRPLAASVGRRADGRGSRP
GGASPYRDMGAPSTSVPQDGCSDGDRARLCRLD
NCSWVL*EEEAPPMAHSVPSDSRTSRRPTTRPHA
ARRAPRGSRRPGRTPKWRLPRISARAPYRLRRL
RRHTYWPPRRPVAASRCWPVGATPLGSVGGRTG
KMDAATLTYDTLRFAEFEDFPETSEPVWILGRKY
SIFTEKDEILSDVASRLWFTYRKNFPAIGGCRD
ISLSSKCGPWRY*VLRGGTGPTSDTGWGCMLRC
GQMIFAQALVCRHLGRDWRWTQRKRQPDSYFSVL
NAFIDRKDSYYSIHQIAQMGVGEGKSIGQWYGP
NTVAQVLKKLAVFDTWSSLAVHIAMDNTVVMEE
IRRLCRTSVPCAGATAFPADSDRHCNGFPAGAEV
TNRPSPWRPLVLLIPLRLGLTDINEAYVETLKH
CFMMPQSLGVIGGKPNSAHYFIGYVGEELIYLD
PHTTQPAVEPTDGCFIPDESFHCQHPPCRMSIAE
LDPSIAVGFFCKTEDDFNDWCQQVKKLSLLGGA
LPMFELVELQPSHLACPDVLNLSLGESCQVQVG
SLGGTICALASPVLAPLVLTIKVCVSLSREHLAV
VRL*DQVWSGASPPPSLRPAAQDPPRLPHQRCL
PGRCGAGRATMESSGVWSRQNMQALW*RSPGWG
TGSLGHHWPWVA*TPRTMASVPQEPAWARAVK*V
AVSASSSSLSPCGKLYKQGNGNGTTPDDHEGQT
RDRGPSGLRLCRPPPALLTLPCSSSLLPPPIFA
GLHNPRTCSHPHGDSCLWAAEQALLSLPHAKGP*
LTPRWPTQDA*CAMSCSF*ILLM*SDWKDSSTQ
KMKTLKSCPFENPGVGGGTCESLGLLVPLRFIH
PARSPAEGCAPCCLPPEGHPLCSWTEAALPGRPY
CLVSDCPAQSARQGLCIRTRSRLLGASRVFSRH
CQPRVSTWASVPLAPRRRFCGWFGIKVLFEVVR
HRHEFLGAP*VRVSEDLCRLA*EERQPHCGPTPK
DWAALEGGAPTAVSSLPSLAYQGLPQWEMRLEE
RRRGSSLLWGEKYSGSGCVGNLKMACTGHSVGG
LGRRAAGCPGAAAGACPQSVPFQCCGILWQLPQV
W*RGGGGASTCDSQA*GWTACLSQEPSPCPCLA
ENCPPMPLRC*VV*GQKGKTT*VLWCVVGRHHR
VASPGGISRTSAPIPAPTTA*PCVTCCPGSQSAL
CPAALLPVLGKYLCPTRKCKQEGLGSVGTFLMS
SYCGVGRTRCCCCCLCRSGSQRSPRTRAGQSAL
TGPPGSRGHPQPPKH*RHSVFPRILSPLGVVGVG
KQGRLVRSPFLGLHVRVHGAPLCWELQW*CVGH
LDQRGALSRVF*KRLNEENVVHS**YSLQFLMA
NNKFQ*KT
Frame 2
RQPRVRASSDQRGAAGPSQRPSAGGQTGGGVAP
AGQVRTATWARRARPYRKMAARTGTELASAAST
TAPGSSKRRKRHPWHTVSRRTAEPAVVPRHDPM
PPAGRPGARVGPAVRQNGGSRVFPLARRIVFAAC
AGTPIGPRGVPSPRRVAGPSERRRSGQSAAGLG
RWTQLL*PTTLSGLLSLKIFLRPQSPFGYWVEN
TAFSQKRTRSCLMWHLDFGLHTGKTFQPLEVVGT
FHSPVNVALGVTES*EGGQAPPRTQAGAACCGV
DR*SLPKPWCAGT*AEIGGGHKGRGSQTATSAS
STHSSTGRTVTTPFTR*RKWELAKASP*ASGTGP
TLSPRS*RSLLSSIRGAPWRSTLQWTTLL*WRK
SEGCAGPAFPVQAPLRFLQIPTGTATDSLPELR
SPTGRRHGDPWYFSFPCAWGSRTSTRPTWRR*ST
AS*CPSPWASSEGSPTAPTTSSATLVRSSSTWT
PTPRSQPWSPLMAASSRTRASTASTRRAA*ASR
SLTRPSLWGFSVRLKMTSMIGASKSKSCLCLEVP
CPCLSWWSCSLHIWPAPTS*TCP*VRAAKSRWG
PSEVRSVPLLPQSWPPWF*PLRCV*A*AVSTWQ
WFACETRYGVERPLLQACAQQPRTHLVFPTSAAC
PGAVELGVLPWSPQGSGADRTCRLCGDAVLGGG
LVHLGTTGHGWRRPLGPWPACRRSRPGLVQ*SE
WP*ARPPHLCLPVGNSTNKAMAMEPLLMTTRVRR
GTEAPQA*DCAGRPLPSSPCPAPLLCSLPPYSQ
VCTTPGPVHTRMGTAVCGLQSRHCSVCPTPRAL
DSHPGGPPKMPDALCPVPSRFF*CRATGKILRLR
R*RL*NPVPLKILGSGVAPVRAWGSWCRCVSSI
PPARLPRAAPRAASPQRATRCARGLRLRCPGGL
TAWCQTAQLRVPVRACASARGAVC*ELPECSLDT
ASPVLAPGPQSHLLPGAGSVVGLELKSCLKLSD
TDMNFWALPESESQKTCAGWRERSGSHTAAPRP
RTGLLSRGARPPLCPLCPAWLTKGYLSGR*GWRN
EGEVPPCFGEKSIQEVGVWET*RWRAQDTAWAA
WAEGRLAVLELLLEPALRVSLSSAVAFCGSFPR
CGDGGGAGPPPVTARLEGGRRASPRSLPHVLALL
RIALPCR*GVRWFRAKRGKPLESCGVWWADTTG
WHHLVAFPEPQPRFQHPPPPDPV*PAVPGPRVH
SAPLLCCLSWESIFAPLGNVNRRAWGAWALFS*A
ATAALAGLAAAAAACVGRGARDPRGRAPDSRH*
PAHLVAEDTPSPPSIEDIVYFLVSFLPWV*LGW
GSREGWCDLHSLGSTSEFMVRRCAGSCSGNVWDT
LTKGELCLVCFEKGLMKRMLFILSSIVCNS*WQ
IISFSRKQ
Sequence XM_001125996
GAGGAGTTGCCGGGGGTCGGTCTTTCCGCAGGAGCTACTCTGACCTACGACACTCTCCGGTTTGCTGAGTTTGAAGATTTTCCTGAGACCTCAGAGCCCG
TTTGGATACTGGGTAGAAAATACAGCATTTTCACAGAAAAGGACGAGATCTTGTCTGATGTGGCATCTAGACTTTGGTTTACATACAGGAAAAACTTTCC
AGCCATTGGAGGTTGTCGGGACATTTCACTCTCCAGTAAATGTGGCCCTTGGCGTTACTGAGTCCTAAGAGGGGGGACAGGCCCCACCTCGGACACAGGC
TGGGGCTGCATGCTGCGGTGTGGACAGATGATCTTTGCCCAAGCCCTGGTGTGCCGGCACCTAGGCCGAGATTGGAGGTGGACACAAAGGAAGAGGCAGC
CAGACAGCTACTTCAGCGTCCTCAACGCATTCATCGACAGGAAGGACAGTTACTACTCCATTCACCAGATAGCGCAAATGGGAGTTGGCGAAGGCAAGTC
CATAGGCCAGTGGTACGGGCCCAACACTGTCGCCCAGGTCCTGAAGAAGCTTGCTGTCTTCGATACGTGGAGCTCCTTGGCGGTCCACATTGCAATGGAC
AACACTGTTGTGATGGAGGAAATCAGAAGGTTGTGCAGGACCAGCGTTCCCTGTGCAGGCGCCACTGCGTTTCCTGCAGATTCCGACCGGCACTGCAACG
GATTCCCTGCCGGAGCTGAGGTCACCAACAGGCCGTCGCCATGGAGACCCCTGGTACTTCTCATTCCCCTGCGCCTGGGGCTCACGGACATCAACGAGGC
CTACGTGGAGACGCTGAAGCACTGCTTCATGATGCCCCAGTCCCTGGGCGTCATCGGAGGGAAGCCCAACAGCGCCCACTACTTCATCGGCTACGTTGGT
GAGGAGCTCATCTACCTGGACCCCCACACCACGCAGCCAGCCGTGGAGCCCACTGATGGCTGCTTCATCCCGGACGAGAGCTTCCACTGCCAGCACCCGC
CGTGCCGCATGAGCATCGCGGAGCTTGACCCGTCCATCGCTGTGGGGTTTTTCTGTAAGACTGAAGATGACTTCAATGATTGGTGCCAGCAAGTCAAAAA
GCTGTCTCTGCTTGGAGGTGCCCTGCCCATGTTTGAGCTGGTGGAGCTGCAGCCTTCACATCTGGCCTGCCCCGACGTCCTGAACCTGTCCCTAGATTCT
TCTGATGTAGAGCGACTGGAAAGATTCTTCGACTCAGAAGATGAAGACTTTGAAATCCTGTCCCTTTGAAAATCCTGGGGTCGGGGGTGGCACCTGTGAG
AGCCTGGGGCTCCTGGTGCCGCTGCGTTTCATCCATCCCGCCCGCTCGCCTGCCGAGGGCTGCGCCCCGTGCTGCCTCCCCCCAGAGGGCCACCCGCTGT
GCTCGTGGACTGAGGCTGCGCTGCCCGGGAGGCCTTACTGCTTGGTGTCAGACTGCCCAGCTCAGAGTGCCCGTCAGGGCCTGTGCATCCGCACGCGGAG
CCGTCTGTTAGGAGCTTCCAGAGTGTTCTCTCGACACTGCCAGCCCCGTGTTAGCACCTGGGCCTCAGTCCCACTTGCTCCCAGGCGCCGGTTCTGTGGT
TGGTTTGGAATTAAAGTCCTGTTTGAAGTTGTCAGACACAGACATGAATTTCTGGGCGCTCCCTGAGTCAGAGTCTCAGAAGACCTGTGCAGGCTGGCGT
GAGAGGAGCGGCAGCCACACTGCGGCCCCACGCCCAAGGACTGGGCTGCTCTCGAGGGGGGCGCGCCCACCGCTGTGTCCTCTCTGCCCAGCCTGGCTTA
CCAAGGGCTACCTCAGTGGGAGATGAGGTTGGAGGAACGAAGGCGAGGTTCCTCCTTGCTTTGGGGAGAAAAGTATTCAGGAAGTGGGTGTGTGGGAAAC
CTGAAGATGGCGTGCACAGGACACAGCGTGGGCGGCCTGGGCAGAAGGGCGGCTGGCTGTCCTGGAGCTGCTGCTGGAGCCTGCCCTCAGAGTGTCCCTT
TCCAGTGCTGTGGCATTCTGTGGCAGCTTCCCCAGGTGTGGTGACGGGGGGGGGGCGGGGCCTCCACCTGTGACAGCCAGGCTTGAGGGTGGACGGCGTG
CCTCTCCCAGGAGCCTTCCCCATGTCCTTGCCTTGCTGAGAATTGCCCTCCCATGCCGCTGAGGTGTTAGGTGGTTTAGGGCCAAAAGGGGAAAACCACT
TGAGTCTTGTGGTGTGTGGTGGGCAGACACCACAGGGTGGCATCACCTGGTGGCATTTCCAGAACCTCAGCCCCGATTCCAGCACCCACCACCGCCTGAC
CCTGTGTAACCTGCTGTCCCGGGTCCCAGAGTGCACTCTGCCCCGCTGCTCTGCTGCCTGTCCTGGGAAAGTATCTTTGCCCCACTAGGAAATGTAAACA
GGAGGGCTTGGGGAGCGTGGGCACTTTTCTCATGAGCAGCTACTGCGGCGTTGGCAGGACTCGCTGCTGCTGCTGCTGCTTGTGTAGGTCGGGGAGCCAG
AGATCCCCGAGGACGCGCGCCGGACAGTCGGCACTGACCGGCCCACCTGGTAGCAGAGGACACCCCCAGCCCCCCAAGCATTGAAGACATAGTGTATTTC
CTCGTATCCTTTCTCCCTTGGGTGTAGTTGGGGTGGGGAAGCAGGGAAGGCTGGTGCGATCTCCATTCCTTGGGCTCCACGTCCGAGTTCATGGTGCGCC
GCTGTGCTGGGAGCTGCAGTGGTAATGTGTGGGACACCTTGACCAAAGGGGAGCTTTGTCTCGTGTGTTTTGAAAAAGGCTTAATGAAGAGAATGTTGTT
CATTCTTAGTAGTATAGTTTGCAATTCTTAATGGCAAATAATAAGTTTCAGTAGAAAACAAA
- refseq_LOC727737.F1 (forward primer)
- refseq_LOC727737.R1 (reverse primer)
- Single exon skipping
Frame 0
EELPGVGLSAGATLTYDTLRFAEFEDFPETSEP
VWILGRKYSIFTEKDEILSDVASRLWFTYRKNFP
AIGGCRDISLSSKCGPWRY*VLRGGTGPTSDTG
WGCMLRCGQMIFAQALVCRHLGRDWRWTQRKRQ
PDSYFSVLNAFIDRKDSYYSIHQIAQMGVGEGKS
IGQWYGPNTVAQVLKKLAVFDTWSSLAVHIAMD
NTVVMEEIRRLCRTSVPCAGATAFPADSDRHCN
GFPAGAEVTNRPSPWRPLVLLIPLRLGLTDINEA
YVETLKHCFMMPQSLGVIGGKPNSAHYFIGYVG
EELIYLDPHTTQPAVEPTDGCFIPDESFHCQHP
PCRMSIAELDPSIAVGFFCKTEDDFNDWCQQVKK
LSLLGGALPMFELVELQPSHLACPDVLNLSLDS
SDVERLERFFDSEDEDFEILSL*KSWGRGWHL*
EPGAPGAAAFHPSRPLACRGLRPVLPPPRGPPAV
LVD*GCAAREALLLGVRLPSSECPSGPVHPHAE
PSVRSFQSVLSTLPAPC*HLGLSPTCSQAPVLW
LVWN*SPV*SCQTQT*ISGRSLSQSLRRPVQAGV
RGAAATLRPHAQGLGCSRGGRAHRCVLSAQPGL
PRATSVGDEVGGTKARFLLALGRKVFRKWVCGK
PEDGVHRTQRGRPGQKGGWLSWSCCWSLPSECPF
PVLWHSVAASPGVVTGGGRGLHL*QPGLRVDGV
PLPGAFPMSLPC*ELPSHAAEVLGGLGPKGENH
LSLVVCGGQTPQGGITWWHFQNLSPDSSTHHRLT
LCNLLSRVPECTLPRCSAACPGKVSLPH*EM*T
GGLGERGHFSHEQLLRRWQDSLLLLLLV*VGEP
EIPEDARRTVGTDRPTW*QRTPPAPQALKT*CIS
SYPFSLGCSWGGEAGKAGAISIPWAPRPSSWCA
AVLGAAVVMCGTP*PKGSFVSCVLKKA**RECC
SFLVV*FAILNGK**VSVENK
Frame 1
RSCRGSVFPQELL*PTTLSGLLSLKIFLRPQSP
FGYWVENTAFSQKRTRSCLMWHLDFGLHTGKTF
QPLEVVGTFHSPVNVALGVTES*EGGQAPPRTQA
GAACCGVDR*SLPKPWCAGT*AEIGGGHKGRGS
QTATSASSTHSSTGRTVTTPFTR*RKWELAKAS
P*ASGTGPTLSPRS*RSLLSSIRGAPWRSTLQWT
TLL*WRKSEGCAGPAFPVQAPLRFLQIPTGTAT
DSLPELRSPTGRRHGDPWYFSFPCAWGSRTSTR
PTWRR*STAS*CPSPWASSEGSPTAPTTSSATLV
RSSSTWTPTPRSQPWSPLMAASSRTRASTASTR
RAA*ASRSLTRPSLWGFSVRLKMTSMIGASKSK
SCLCLEVPCPCLSWWSCSLHIWPAPTS*TCP*IL
LM*SDWKDSSTQKMKTLKSCPFENPGVGGGTCE
SLGLLVPLRFIHPARSPAEGCAPCCLPPEGHPL
CSWTEAALPGRPYCLVSDCPAQSARQGLCIRTRS
RLLGASRVFSRHCQPRVSTWASVPLAPRRRFCG
WFGIKVLFEVVRHRHEFLGAP*VRVSEDLCRLA
*EERQPHCGPTPKDWAALEGGAPTAVSSLPSLAY
QGLPQWEMRLEERRRGSSLLWGEKYSGSGCVGN
LKMACTGHSVGGLGRRAAGCPGAAAGACPQSVP
FQCCGILWQLPQVW*RGGGGASTCDSQA*GWTAC
LSQEPSPCPCLAENCPPMPLRC*VV*GQKGKTT
*VLWCVVGRHHRVASPGGISRTSAPIPAPTTA*
PCVTCCPGSQSALCPAALLPVLGKYLCPTRKCKQ
EGLGSVGTFLMSSYCGVGRTRCCCCCLCRSGSQ
RSPRTRAGQSALTGPPGSRGHPQPPKH*RHSVF
PRILSPLGVVGVGKQGRLVRSPFLGLHVRVHGAP
LCWELQW*CVGHLDQRGALSRVF*KRLNEENVV
HS**YSLQFLMANNKFQ*KT
Frame 2
GVAGGRSFRRSYSDLRHSPVC*V*RFS*DLRAR
LDTG*KIQHFHRKGRDLV*CGI*TLVYIQEKLS
SHWRLSGHFTLQ*MWPLALLSPKRGDRPHLGHR
LGLHAAVWTDDLCPSPGVPAPRPRLEVDTKEEAA
RQLLQRPQRIHRQEGQLLLHSPDSANGSWRRQV
HRPVVRAQHCRPGPEEACCLRYVELLGGPHCNG
QHCCDGGNQKVVQDQRSLCRRHCVSCRFRPALQR
IPCRS*GHQQAVAMETPGTSHSPAPGAHGHQRG
LRGDAEALLHDAPVPGRHRREAQQRPLLHRLRW
*GAHLPGPPHHAASRGAH*WLLHPGRELPLPAPA
VPHEHRGA*PVHRCGVFL*D*R*LQ*LVPASQK
AVSAWRCPAHV*AGGAAAFTSGLPRRPEPVPRF
F*CRATGKILRLRR*RL*NPVPLKILGSGVAPVR
AWGSWCRCVSSIPPARLPRAAPRAASPQRATRC
ARGLRLRCPGGLTAWCQTAQLRVPVRACASARG
AVC*ELPECSLDTASPVLAPGPQSHLLPGAGSVV
GLELKSCLKLSDTDMNFWALPESESQKTCAGWR
ERSGSHTAAPRPRTGLLSRGARPPLCPLCPAWL
TKGYLSGR*GWRNEGEVPPCFGEKSIQEVGVWET
*RWRAQDTAWAAWAEGRLAVLELLLEPALRVSL
SSAVAFCGSFPRCGDGGGAGPPPVTARLEGGRR
ASPRSLPHVLALLRIALPCR*GVRWFRAKRGKPL
ESCGVWWADTTGWHHLVAFPEPQPRFQHPPPPD
PV*PAVPGPRVHSAPLLCCLSWESIFAPLGNVN
RRAWGAWALFS*AATAALAGLAAAAAACVGRGAR
DPRGRAPDSRH*PAHLVAEDTPSPPSIEDIVYF
LVSFLPWV*LGWGSREGWCDLHSLGSTSEFMVR
RCAGSCSGNVWDTLTKGELCLVCFEKGLMKRMLF
ILSSIVCNS*WQIISFSRKQ