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Sequences

Sequence NM_005501

AGGTGAACAGGTCCTCACGCCCAGCTCCGCGCCCTCACGCGCTCTCGCCGGGACCCCGCTTCCGCTGGCAGCCATGGGCCCCGGCCCCAGCCGCGCGCCC
CGCGCCCCACGCCTGATGCTCTGTGCGCTCGCCTTGATGGTGGCGGCCGGCGGCTGCGTCGTCTCCGCCTTCAACCTGGATACCCGATTCCTGGTAGTGA
AGGAGGCCGGGAACCCGGGCAGCCTCTTCGGCTACTCGGTCGCCCTCCATCGGCAGACAGAGCGGCAGCAGCGCTACCTGCTCCTGGCTGGTGCCCCCCG
GGAGCTCGCTGTGCCCGATGGCTACACCAACCGGACTGGTGCTGTGTACCTGTGCCCACTCACTGCCCACAAGGATGACTGTGAGCGGATGAACATCACA
GTGAAAAATGACCCTGGCCATCACATTATTGAGGACATGTGGCTTGGAGTGACTGTGGCCAGCCAGGGCCCTGCAGGCAGAGTTCTGGTCTGTGCCCACC
GCTACACCCAGGTGCTGTGGTCAGGGTCAGAAGACCAGCGGCGCATGGTGGGCAAGTGCTACGTGCGAGGCAATGACCTAGAGCTGGACTCCAGTGATGA
CTGGCAGACCTACCACAACGAGATGTGCAATAGCAACACAGACTACCTGGAGACGGGCATGTGCCAGCTGGGCACCAGCGGTGGCTTCACCCAGAACACT
GTGTACTTCGGCGCCCCCGGTGCCTACAACTGGAAAGGAAACAGCTACATGATTCAGCGCAAGGAGTGGGACTTATCTGAGTATAGTTACAAGGACCCAG
AGGACCAAGGAAACCTCTATATTGGGTACACGATGCAGGTAGGCAGCTTCATCCTGCACCCCAAAAACATCACCATTGTGACAGGTGCCCCACGGCACCG
ACATATGGGCGCGGTGTTCTTGCTGAGCCAGGAGGCAGGCGGAGACCTGCGGAGGAGGCAGGTGCTGGAGGGCTCGCAGGTGGGCGCCTATTTTGGCAGC
GCCATTGCCCTGGCAGACCTGAACAATGATGGGTGGCAGGACCTCCTGGTGGGCGCCCCCTACTACTTCGAGAGGAAAGAGGAAGTAGGGGGTGCCATCT
ATGTCTTCATGAACCAGGCGGGAACCTCCTTCCCTGCTCACCCCTCACTCCTTCTTCATGGCCCCAGTGGCTCTGCCTTTGGTTTATCTGTGGCCAGCAT
TGGTGACATCAACCAGGATGGATTTCAGGATATTGCTGTGGGAGCTCCGTTTGAAGGCTTGGGCAAAGTGTACATCTATCACAGTAGCTCTAAGGGGCTC
CTTAGACAGCCCCAGCAGGTAATCCATGGAGAGAAGCTGGGACTGCCTGGGTTGGCCACCTTCGGCTATTCCCTCAGTGGGCAGATGGATGTGGATGAGA
ACTTCTACCCAGACCTTCTAGTGGGAAGCCTGTCAGACCACATTGTGCTGCTGCGGGCCCGGCCCGTCATCAACATCGTCCACAAGACCTTGGTGCCCAG
GCCAGCTGTGCTGGACCCTGCACTTTGCACGGCCACCTCTTGTGTGCAAGTGGAGCTGTGCTTTGCTTACAACCAGAGTGCCGGGAACCCCAACTACAGG
CGAAACATCACCCTGGCCTACACTCTGGAGGCTGACAGGGACCGCCGGCCGCCCCGGCTCCGCTTTGCCGGCAGTGAGTCCGCTGTCTTCCACGGCTTCT
TCTCCATGCCCGAGATGCGCTGCCAGAAGCTGGAGCTGCTCCTGATGGACAACCTCCGTGACAAACTCCGCCCCATCATCATCTCCATGAACTACTCTTT
ACCTTTGCGGATGCCCGATCGCCCCCGGCTGGGGCTGCGGTCCCTGGACGCCTACCCGATCCTCAACCAGGCACAGGCTCTGGAGAACCACACTGAGGTC
CAGTTCCAGAAGGAGTGCGGGCCTGACAACAAGTGTGAGAGCAACTTGCAGATGCGGGCAGCCTTCGTGTCAGAGCAGCAGCAGAAGCTGAGCAGGCTCC
AGTACAGCAGAGACGTCCGGAAATTGCTCCTGAGCATCAACGTGACGAACACCCGGACCTCGGAGCGCTCCGGGGAGGACGCCCACGAGGCGCTGCTCAC
CCTGGTGGTGCCTCCCGCCCTGCTGCTGTCCTCAGTGCGCCCCCCCGGGGCCTGCCAAGCTAATGAGACCATCTTTTGCGAGCTGGGGAACCCCTTCAAA
CGGAACCAGAGGATGGAGCTGCTCATCGCCTTTGAGGTCATCGGGGTGACCCTGCACACAAGGGACCTTCAGGTGCAGCTGCAGCTCTCCACGTCGAGTC
ACCAGGACAACCTGTGGCCCATGATCCTCACTCTGCTGGTGGACTATACACTCCAGACCTCGCTTAGCATGGTAAATCACCGGCTACAAAGCTTCTTTGG
GGGGACAGTGATGGGTGAGTCTGGCATGAAAACTGTGGAGGATGTAGGAAGCCCCCTCAAGTATGAATTCCAGGTGGGCCCAATGGGGGAGGGGCTGGTG
GGCCTGGGGACCCTGGTCCTAGGTCTGGAGTGGCCCTACGAAGTCAGCAATGGCAAGTGGCTGCTGTATCCCACGGAGATCACCGTCCATGGCAATGGGT
CCTGGCCCTGCCGACCACCTGGAGACCTTATCAACCCTCTCAACCTCACTCTTTCTGACCCTGGGGACAGGCCATCATCCCCACAGCGCAGGCGGCGACA
GCTGGATCCAGGGGGAGGCCAGGGCCCCCCACCTGTCACTCTGGCTGCTGCCAAAAAAGCCAAGTCTGAGACTGTGCTGACCTGTGCCACAGGGCGTGCC
CACTGTGTGTGGCTAGAGTGCCCCATCCCTGATGCCCCCGTTGTCACCAACGTGACTGTGAAGGCACGAGTGTGGAACAGCACCTTCATCGAGGATTACA
GAGACTTTGACCGAGTCCGGGTAAATGGCTGGGCTACCCTATTCCTCCGAACCAGCATCCCCACCATCAACATGGAGAACAAGACCACGTGGTTCTCTGT
GGACATTGACTCGGA
GCTGGTGGAGGAGCTGCCGGCCGAAATCGAGCTGTGGCTGGTGCTGGTGGCCGTGGGTGCAGGGCTGCTGCTGCTGGGGCTGATC
ATCCTCCTGCTGTGGAAGTGTGACTTCTTTAAGCGGACCCGCTATTATCAGATCATGCCCAAGTACCACGCAGTGCGGATCCGGGAGGAGGAGCGCTACC
CACCTCCAGGGAGCACCCTGCCCACCAAGAAGCACTGGGTGACCAGCTGGCAGACTCGGGACCAATACTACTGACGTCCTCCCTGATCCCACCCCCTCCT
CCCCCAGTGTCCCCTTTCTTCCTATTTATCATAAGTTATGCCTCTGACAGTCCACAGGGGCCACCACCTTTGGCTGGTAGCAGCAGGCTCAGGCACATAC
ACCTCGTCAAGAGCATGCACATGCTGTCTGGCCCTGGGGATCTTCCCACAGGAGGGCCAGCGCTGTGGACCTTACAACGCCGAGTGCACTGCATTCCTGT
GCCCTAGATGCACGTGGGGCCCACTGCTCGTGGACTGTGCTGGTGCATCACGGATGGTGCATGGGCTCGCCGTGTCTCAGCCTCTGCCAGCGCCAAAACA
AGCCAAAGAGCCTCCCACCAGAGCCGGGAGGAAAAGGCCCCTGCAATGTGGTGACACCTCCCCCTTTCACACTGGATCCATCTTGAGCCACAGTCACTGG
ATTGACTTTGCTGTCAAAACTACTGACAGGGAGCAGCCCCCGGGCCGCTGGCTGGTGGGCCCCCAATGACACCCATGCCAGAGAGGTGGGGATCCTGCCT
AAGGTTGTCTACGGGGGCACTTGGAGGACCTGGCGTGCTCAGACCCAACAGCAAAGGAACTAGAAAGAAGGACCCAGAACGGCTTGCTTTCCTGCATCTC
TGTGAAGCCTCTCTCCTTGGCCACAGACTGAACTCGCAGGGAATGCAGCAGGAAGGAACAAAGACAGGCAAACGGCAACGTAGCCTGGGCTCACTGTGCT
GGGGCACGGCGGGATCCTCCACAGAGAGGAGGGGACCAATTCTGGACAGACAGATGTTGGGAGGATACAGAGGAGATGCCACTTCTCACTCACCACTACC
AGCCAGCCTCAGAAGGCCCCAGAGAGACCCTGCAAGACCACGGAGGGAGCGACACTTGAATGTAGAATAGGCAGGGGGCCCTGCCCCACCCCATCCAGCC
AGACCCCACGCTGACCATGCGTCAGGGGCCTAGAGGTGGAGTTCTTAGCTATCCTTGGCTTTCAGAGCCAGCCTGGCTCTGCCCCCTCCCCCATGGGCTG
TGTCCTAAGGCCCATTTGAGAAGCTGAGGCTAGTTCCAGAAAACCTCTCCTGACCCCTGCCTGTTGGCAGGCCCACTCCCCAGCCCCAGCCCCTTCCATG
GTACTGTAGCAGGGGAATTCCCTCCCCCTCCTTGTGCCTTCTTTGTATATAGGCTTCTCACGGCGACCAATAAACAGCTCCCAGTTTGT
  • refseq_ITGA3.F1 (forward primer)
  • refseq_ITGA3.R1 (reverse primer)
  • Single exon skipping

Frame 0

R*TGPHAQLRALTRSRRDPASAGSHGPRPQPRA
PRPTPDALCARLDGGGRRLRRLRLQPGYPIPGSE
GGREPGQPLRLLGRPPSADRAAAALPAPGWCPP
GARCARWLHQPDWCCVPVPTHCPQG*L*ADEHH
SEK*PWPSHY*GHVAWSDCGQPGPCRQSSGLCPP
LHPGAVVRVRRPAAHGGQVLRARQ*PRAGLQ**
LADLPQRDVQ*QHRLPGDGHVPAGHQRWLHPEH
CVLRRPRCLQLERKQLHDSAQGVGLI*V*LQGPR
GPRKPLYWVHDAGRQLHPAPQKHHHCDRCPTAP
TYGRGVLAEPGGRRRPAEEAGAGGLAGGRLFWQ
RHCPGRPEQ*WVAGPPGGRPLLLREERGSRGCHL
CLHEPGGNLLPCSPLTPSSWPQWLCLWFICGQH
W*HQPGWISGYCCGSSV*RLGQSVHLSQ*L*GA
P*TAPAGNPWREAGTAWVGHLRLFPQWADGCG*E
LLPRPSSGKPVRPHCAAAGPARHQHRPQDLGAQ
ASCAGPCTLHGHLLCASGAVLCLQPECREPQLQ
AKHHPGLHSGG*QGPPAAPAPLCRQ*VRCLPRLL
LHARDALPEAGAAPDGQPP*QTPPHHHLHELLF
TFADARSPPAGAAVPGRLPDPQPGTGSGEPH*G
PVPEGVRA*QQV*EQLADAGSLRVRAAAEAEQAP
VQQRRPEIAPEHQRDEHPDLGALRGGRPRGAAH
PGGASRPAAVLSAPPRGLPS**DHLLRAGEPLQ
TEPEDGAAHRL*GHRGDPAHKGPSGAAAALHVES
PGQPVAHDPHSAGGLYTPDLA*HGKSPATKLLW
GDSDG*VWHENCGGCRKPPQV*IPGGPNGGGAG
GPGDPGPRSGVALRSQQWQVAAVSHGDHRPWQWV
LALPTTWRPYQPSQPHSF*PWGQAIIPTAQAAT
AGSRGRPGPPTCHSGCCQKSQV*DCADLCHRAC
PLCVARVPHP*CPRCHQRDCEGTSVEQHLHRGLQ
RL*PSPGKWLGYPIPPNQHPHHQHGEQDHVVLC
GH*LG
AGGGAAGRNRAVAGAGGRGCRAAAAGAD
HPPAVEV*LL*ADPLLSDHAQVPRSADPGGGALP
TSREHPAHQEALGDQLADSGPILLTSSLIPPPP
PPVSPFFLFIISYASDSPQGPPPLAGSSRLRHI
HLVKSMHMLSGPGDLPTGGPALWTLQRRVHCIPV
P*MHVGPTARGLCWCITDGAWARRVSASASAKT
SQRASHQSREEKAPAMW*HLPLSHWIHLEPQSL
D*LCCQNY*QGAAPGPLAGGPPMTPMPERWGSCL
RLSTGALGGPGVLRPNSKGTRKKDPERLAFLHL
CEASLLGHRLNSQGMQQEGTKTGKRQRSLGSLC
WGTAGSSTERRGPILDRQMLGGYRGDATSHSPLP
ASLRRPQRDPARPRRERHLNVE*AGGPAPPHPA
RPHADHASGA*RWSS*LSLAFRASLALPPPPWA
VS*GPFEKLRLVPENLS*PLPVGRPTPQPQPLPW
YCSRGIPSPSLCLLCI*ASHGDQ*TAPSL

Frame 1

GEQVLTPSSAPSRALAGTPLPLAAMGPGPSRAP
RAPRLMLCALALMVAAGGCVVSAFNLDTRFLVV
KEAGNPGSLFGYSVALHRQTERQQRYLLLAGAPR
ELAVPDGYTNRTGAVYLCPLTAHKDDCERMNIT
VKNDPGHHIIEDMWLGVTVASQGPAGRVLVCAH
RYTQVLWSGSEDQRRMVGKCYVRGNDLELDSSDD
WQTYHNEMCNSNTDYLETGMCQLGTSGGFTQNT
VYFGAPGAYNWKGNSYMIQRKEWDLSEYSYKDP
EDQGNLYIGYTMQVGSFILHPKNITIVTGAPRHR
HMGAVFLLSQEAGGDLRRRQVLEGSQVGAYFGS
AIALADLNNDGWQDLLVGAPYYFERKEEVGGAI
YVFMNQAGTSFPAHPSLLLHGPSGSAFGLSVASI
GDINQDGFQDIAVGAPFEGLGKVYIYHSSSKGL
LRQPQQVIHGEKLGLPGLATFGYSLSGQMDVDE
NFYPDLLVGSLSDHIVLLRARPVINIVHKTLVPR
PAVLDPALCTATSCVQVELCFAYNQSAGNPNYR
RNITLAYTLEADRDRRPPRLRFAGSESAVFHGF
FSMPEMRCQKLELLLMDNLRDKLRPIIISMNYSL
PLRMPDRPRLGLRSLDAYPILNQAQALENHTEV
QFQKECGPDNKCESNLQMRAAFVSEQQQKLSRL
QYSRDVRKLLLSINVTNTRTSERSGEDAHEALLT
LVVPPALLLSSVRPPGACQANETIFCELGNPFK
RNQRMELLIAFEVIGVTLHTRDLQVQLQLSTSS
HQDNLWPMILTLLVDYTLQTSLSMVNHRLQSFFG
GTVMGESGMKTVEDVGSPLKYEFQVGPMGEGLV
GLGTLVLGLEWPYEVSNGKWLLYPTEITVHGNG
SWPCRPPGDLINPLNLTLSDPGDRPSSPQRRRRQ
LDPGGGQGPPPVTLAAAKKAKSETVLTCATGRA
HCVWLECPIPDAPVVTNVTVKARVWNSTFIEDY
RDFDRVRVNGWATLFLRTSIPTINMENKTTWFSV
DIDSE
LVEELPAEIELWLVLVAVGAGLLLLGLI
ILLLWKCDFFKRTRYYQIMPKYHAVRIREEERY
PPPGSTLPTKKHWVTSWQTRDQYY*RPP*SHPLL
PQCPLSSYLS*VMPLTVHRGHHLWLVAAGSGTY
TSSRACTCCLALGIFPQEGQRCGPYNAECTAFL
CPRCTWGPLLVDCAGASRMVHGLAVSQPLPAPKQ
AKEPPTRAGRKRPLQCGDTSPFHTGSILSHSHW
IDFAVKTTDREQPPGRWLVGPQ*HPCQRGGDPA
*GCLRGHLEDLACSDPTAKELERRTQNGLLSCIS
VKPLSLATD*TRRECSRKEQRQANGNVAWAHCA
GARRDPPQRGGDQFWTDRCWEDTEEMPLLTHHY
QPASEGPRETLQDHGGSDT*M*NRQGALPHPIQP
DPTLTMRQGPRGGVLSYPWLSEPAWLCPLPHGL
CPKAHLRS*G*FQKTSPDPCLLAGPLPSPSPFH
GTVAGEFPPPPCAFFVYRLLTATNKQLPVC

Frame 2

VNRSSRPAPRPHALSPGPRFRWQPWAPAPAARP
APHA*CSVRSP*WWRPAAASSPPSTWIPDSW**
RRPGTRAASSATRSPSIGRQSGSSATCSWLVPP
GSSLCPMATPTGLVLCTCAHSLPTRMTVSG*TSQ
*KMTLAITLLRTCGLE*LWPARALQAEFWSVPT
ATPRCCGQGQKTSGAWWASATCEAMT*SWTPVM
TGRPTTTRCAIATQTTWRRACASWAPAVASPRTL
CTSAPPVPTTGKETAT*FSARSGTYLSIVTRTQ
RTKETSILGTRCR*AASSCTPKTSPL*QVPHGT
DIWARCSC*ARRQAETCGGGRCWRARRWAPILAA
PLPWQT*TMMGGRTSWWAPPTTSRGKRK*GVPS
MSS*TRREPPSLLTPHSFFMAPVALPLVYLWPA
LVTSTRMDFRILLWELRLKAWAKCTSITVALRGS
LDSPSR*SMERSWDCLGWPPSAIPSVGRWMWMR
TSTQTF*WEACQTTLCCCGPGPSSTSSTRPWCP
GQLCWTLHFARPPLVCKWSCALLTTRVPGTPTTG
ETSPWPTLWRLTGTAGRPGSALPAVSPLSSTAS
SPCPRCAARSWSCS*WTTSVTNSAPSSSP*TTL
YLCGCPIAPGWGCGPWTPTRSSTRHRLWRTTLRS
SSRRSAGLTTSVRATCRCGQPSCQSSSRS*AGS
STAETSGNCS*AST*RTPGPRSAPGRTPTRRCS
PWWCLPPCCCPQCAPPGPAKLMRPSFASWGTPSN
GTRGWSCSSPLRSSG*PCTQGTFRCSCSSPRRV
TRTTCGP*SSLCWWTIHSRPRLAW*ITGYKASL
GGQ*WVSLA*KLWRM*EAPSSMNSRWAQWGRGWW
AWGPWS*VWSGPTKSAMASGCCIPRRSPSMAMG
PGPADHLETLSTLSTSLFLTLGTGHHPHSAGGD
SWIQGEARAPHLSLWLLPKKPSLRLC*PVPQGVP
TVCG*SAPSLMPPLSPT*L*RHECGTAPSSRIT
ETLTESG*MAGLPYSSEPASPPSTWRTRPRGSL
WTLTR
SWWRSCRPKSSCGWCWWPWVQGCCCWG*S
SSCCGSVTSLSGPAIIRSCPSTTQCGSGRRSAT
HLQGAPCPPRSTG*PAGRLGTNTTDVLPDPTPS
SPSVPFLPIYHKLCL*QSTGATTFGW*QQAQAHT
PRQEHAHAVWPWGSSHRRASAVDLTTPSALHSC
ALDARGAHCSWTVLVHHGWCMGSPCLSLCQRQN
KPKSLPPEPGGKGPCNVVTPPPFTLDPS*ATVTG
LTLLSKLLTGSSPRAAGWWAPNDTHAREVGILP
KVVYGGTWRTWRAQTQQQRN*KEGPRTACFPAS
L*SLSPWPQTELAGNAAGRNKDRQTAT*PGLTVL
GHGGILHREEGTNSGQTDVGRIQRRCHFSLTTT
SQPQKAPERPCKTTEGATLECRIGRGPCPTPSS
QTPR*PCVRGLEVEFLAILGFQSQPGSAPSPMGC
VLRPI*EAEASSRKPLLTPACWQAHSPAPAPSM
VL*QGNSLPLLVPSLYIGFSRRPINSSQF

Sequence NM_002204

AGGTGAACAGGTCCTCACGCCCAGCTCCGCGCCCTCACGCGCTCTCGCCGGGACCCCGCTTCCGCTGGCAGCCATGGGCCCCGGCCCCAGCCGCGCGCCC
CGCGCCCCACGCCTGATGCTCTGTGCGCTCGCCTTGATGGTGGCGGCCGGCGGCTGCGTCGTCTCCGCCTTCAACCTGGATACCCGATTCCTGGTAGTGA
AGGAGGCCGGGAACCCGGGCAGCCTCTTCGGCTACTCGGTCGCCCTCCATCGGCAGACAGAGCGGCAGCAGCGCTACCTGCTCCTGGCTGGTGCCCCCCG
GGAGCTCGCTGTGCCCGATGGCTACACCAACCGGACTGGTGCTGTGTACCTGTGCCCACTCACTGCCCACAAGGATGACTGTGAGCGGATGAACATCACA
GTGAAAAATGACCCTGGCCATCACATTATTGAGGACATGTGGCTTGGAGTGACTGTGGCCAGCCAGGGCCCTGCAGGCAGAGTTCTGGTCTGTGCCCACC
GCTACACCCAGGTGCTGTGGTCAGGGTCAGAAGACCAGCGGCGCATGGTGGGCAAGTGCTACGTGCGAGGCAATGACCTAGAGCTGGACTCCAGTGATGA
CTGGCAGACCTACCACAACGAGATGTGCAATAGCAACACAGACTACCTGGAGACGGGCATGTGCCAGCTGGGCACCAGCGGTGGCTTCACCCAGAACACT
GTGTACTTCGGCGCCCCCGGTGCCTACAACTGGAAAGGAAACAGCTACATGATTCAGCGCAAGGAGTGGGACTTATCTGAGTATAGTTACAAGGACCCAG
AGGACCAAGGAAACCTCTATATTGGGTACACGATGCAGGTAGGCAGCTTCATCCTGCACCCCAAAAACATCACCATTGTGACAGGTGCCCCACGGCACCG
ACATATGGGCGCGGTGTTCTTGCTGAGCCAGGAGGCAGGCGGAGACCTGCGGAGGAGGCAGGTGCTGGAGGGCTCGCAGGTGGGCGCCTATTTTGGCAGC
GCCATTGCCCTGGCAGACCTGAACAATGATGGGTGGCAGGACCTCCTGGTGGGCGCCCCCTACTACTTCGAGAGGAAAGAGGAAGTAGGGGGTGCCATCT
ATGTCTTCATGAACCAGGCGGGAACCTCCTTCCCTGCTCACCCCTCACTCCTTCTTCATGGCCCCAGTGGCTCTGCCTTTGGTTTATCTGTGGCCAGCAT
TGGTGACATCAACCAGGATGGATTTCAGGATATTGCTGTGGGAGCTCCGTTTGAAGGCTTGGGCAAAGTGTACATCTATCACAGTAGCTCTAAGGGGCTC
CTTAGACAGCCCCAGCAGGTAATCCATGGAGAGAAGCTGGGACTGCCTGGGTTGGCCACCTTCGGCTATTCCCTCAGTGGGCAGATGGATGTGGATGAGA
ACTTCTACCCAGACCTTCTAGTGGGAAGCCTGTCAGACCACATTGTGCTGCTGCGGGCCCGGCCCGTCATCAACATCGTCCACAAGACCTTGGTGCCCAG
GCCAGCTGTGCTGGACCCTGCACTTTGCACGGCCACCTCTTGTGTGCAAGTGGAGCTGTGCTTTGCTTACAACCAGAGTGCCGGGAACCCCAACTACAGG
CGAAACATCACCCTGGCCTACACTCTGGAGGCTGACAGGGACCGCCGGCCGCCCCGGCTCCGCTTTGCCGGCAGTGAGTCCGCTGTCTTCCACGGCTTCT
TCTCCATGCCCGAGATGCGCTGCCAGAAGCTGGAGCTGCTCCTGATGGACAACCTCCGTGACAAACTCCGCCCCATCATCATCTCCATGAACTACTCTTT
ACCTTTGCGGATGCCCGATCGCCCCCGGCTGGGGCTGCGGTCCCTGGACGCCTACCCGATCCTCAACCAGGCACAGGCTCTGGAGAACCACACTGAGGTC
CAGTTCCAGAAGGAGTGCGGGCCTGACAACAAGTGTGAGAGCAACTTGCAGATGCGGGCAGCCTTCGTGTCAGAGCAGCAGCAGAAGCTGAGCAGGCTCC
AGTACAGCAGAGACGTCCGGAAATTGCTCCTGAGCATCAACGTGACGAACACCCGGACCTCGGAGCGCTCCGGGGAGGACGCCCACGAGGCGCTGCTCAC
CCTGGTGGTGCCTCCCGCCCTGCTGCTGTCCTCAGTGCGCCCCCCCGGGGCCTGCCAAGCTAATGAGACCATCTTTTGCGAGCTGGGGAACCCCTTCAAA
CGGAACCAGAGGATGGAGCTGCTCATCGCCTTTGAGGTCATCGGGGTGACCCTGCACACAAGGGACCTTCAGGTGCAGCTGCAGCTCTCCACGTCGAGTC
ACCAGGACAACCTGTGGCCCATGATCCTCACTCTGCTGGTGGACTATACACTCCAGACCTCGCTTAGCATGGTAAATCACCGGCTACAAAGCTTCTTTGG
GGGGACAGTGATGGGTGAGTCTGGCATGAAAACTGTGGAGGATGTAGGAAGCCCCCTCAAGTATGAATTCCAGGTGGGCCCAATGGGGGAGGGGCTGGTG
GGCCTGGGGACCCTGGTCCTAGGTCTGGAGTGGCCCTACGAAGTCAGCAATGGCAAGTGGCTGCTGTATCCCACGGAGATCACCGTCCATGGCAATGGGT
CCTGGCCCTGCCGACCACCTGGAGACCTTATCAACCCTCTCAACCTCACTCTTTCTGACCCTGGGGACAGGCCATCATCCCCACAGCGCAGGCGGCGACA
GCTGGATCCAGGGGGAGGCCAGGGCCCCCCACCTGTCACTCTGGCTGCTGCCAAAAAAGCCAAGTCTGAGACTGTGCTGACCTGTGCCACAGGGCGTGCC
CACTGTGTGTGGCTAGAGTGCCCCATCCCTGATGCCCCCGTTGTCACCAACGTGACTGTGAAGGCACGAGTGTGGAACAGCACCTTCATCGAGGATTACA
GAGACTTTGACCGAGTCCGGGTAAATGGCTGGGCTACCCTATTCCTCCGAACCAGCATCCCCACCATCAACATGGAGAACAAGACCACGTGGTTCTCTGT
GGACATTGACTCGGA
GCTGGTGGAGGAGCTGCCGGCCGAAATCGAGCTGTGGCTGGTGCTGGTGGCCGTGGGTGCAGGGCTGCTGCTGCTGGGGCTGATC
ATCCTCCTGCTGTGGAAGTGCGGCTTCTTCAAGCGAGCCCGCACTCGCGCCCTGTATGAAGCTAAGAGGCAGAAGGCGGAGATGAAGAGCCAGCCGTCAG
AGACAGAGAGGCTGACCGACGACTACTGAGGGGGCAGCCCCCCGCCCCCGGCCCACCTGG
TGTGACTTCTTTAAGCGGACCCGCTATTATCAGATCATGC
CCAAGTACCAC
GCAGTGCGGATCCGGGAGGAGGAGCGCTACCCACCTCCAGGGAGCACCCTGCCCACCAAGAAGCACTGGGTGACCAGCTGGCAGACTCG
GGACCAATACTACTGACGTCCTCCCTGATCCCACCCCCTCCTCCCCCAGTGTCCCCTTTCTTCCTATTTATCATAAGTTATGCCTCTGACAGTCCACAGG
GGCCACCACCTTTGGCTGGTAGCAGCAGGCTCAGGCACATACACCTCGTCAAGAGCATGCACATGCTGTCTGGCCCTGGGGATCTTCCCACAGGAGGGCC
AGCGCTGTGGACCTTACAACGCCGAGTGCACTGCATTCCTGTGCCCTAGATGCACGTGGGGCCCACTGCTCGTGGACTGTGCTGGTGCATCACGGATGGT
GCATGGGCTCGCCGTGTCTCAGCCTCTGCCAGCGCCAAAACAAGCCAAAGAGCCTCCCACCAGAGCCGGGAGGAAAAGGCCCCTGCAATGTGGTGACACC
TCCCCCTTTCACACTGGATCCATCTTGAGCCACAGTCACTGGATTGACTTTGCTGTCAAAACTACTGACAGGGAGCAGCCCCCGGGCCGCTGGCTGGTGG
GCCCCCAATGACACCCATGCCAGAGAGGTGGGGATCCTGCCTAAGGTTGTCTACGGGGGCACTTGGAGGACCTGGCGTGCTCAGACCCAACAGCAAAGGA
ACTAGAAAGAAGGACCCAGAACGGCTTGCTTTCCTGCATCTCTGTGAAGCCTCTCTCCTTGGCCACAGACTGAACTCGCAGGGAATGCAGCAGGAAGGAA
CAAAGACAGGCAAACGGCAACGTAGCCTGGGCTCACTGTGCTGGGGCACGGCGGGATCCTCCACAGAGAGGAGGGGACCAATTCTGGACAGACAGATGTT
GGGAGGATACAGAGGAGATGCCACTTCTCACTCACCACTACCAGCCAGCCTCAGAAGGCCCCAGAGAGACCCTGCAAGACCACGGAGGGAGCGACACTTG
AATGTAGAATAGGCAGGGGGCCCTGCCCCACCCCATCCAGCCAGACCCCACGCTGACCATGCGTCAGGGGCCTAGAGGTGGAGTTCTTAGCTATCCTTGG
CTTTCAGAGCCAGCCTGGCTCTGCCCCCTCCCCCATGGGCTGTGTCCTAAGGCCCATTTGAGAAGCTGAGGCTAGTTCCAGAAAACCTCTCCTGACCCCT
GCCTGTTGGCAGGCCCACTCCCCAGCCCCAGCCCCTTCCATGGTACTGTAGCAGGGGAATTCCCTCCCCCTCCTTGTGCCTTCTTTGTATATAGGCTTCT
CACGGCGACCAATAAACAGCTCCCAGTTTGT
  • refseq_ITGA3.F1 (forward primer)
  • refseq_ITGA3.R1 (reverse primer)
  • Single exon skipping

Frame 0

R*TGPHAQLRALTRSRRDPASAGSHGPRPQPRA
PRPTPDALCARLDGGGRRLRRLRLQPGYPIPGSE
GGREPGQPLRLLGRPPSADRAAAALPAPGWCPP
GARCARWLHQPDWCCVPVPTHCPQG*L*ADEHH
SEK*PWPSHY*GHVAWSDCGQPGPCRQSSGLCPP
LHPGAVVRVRRPAAHGGQVLRARQ*PRAGLQ**
LADLPQRDVQ*QHRLPGDGHVPAGHQRWLHPEH
CVLRRPRCLQLERKQLHDSAQGVGLI*V*LQGPR
GPRKPLYWVHDAGRQLHPAPQKHHHCDRCPTAP
TYGRGVLAEPGGRRRPAEEAGAGGLAGGRLFWQ
RHCPGRPEQ*WVAGPPGGRPLLLREERGSRGCHL
CLHEPGGNLLPCSPLTPSSWPQWLCLWFICGQH
W*HQPGWISGYCCGSSV*RLGQSVHLSQ*L*GA
P*TAPAGNPWREAGTAWVGHLRLFPQWADGCG*E
LLPRPSSGKPVRPHCAAAGPARHQHRPQDLGAQ
ASCAGPCTLHGHLLCASGAVLCLQPECREPQLQ
AKHHPGLHSGG*QGPPAAPAPLCRQ*VRCLPRLL
LHARDALPEAGAAPDGQPP*QTPPHHHLHELLF
TFADARSPPAGAAVPGRLPDPQPGTGSGEPH*G
PVPEGVRA*QQV*EQLADAGSLRVRAAAEAEQAP
VQQRRPEIAPEHQRDEHPDLGALRGGRPRGAAH
PGGASRPAAVLSAPPRGLPS**DHLLRAGEPLQ
TEPEDGAAHRL*GHRGDPAHKGPSGAAAALHVES
PGQPVAHDPHSAGGLYTPDLA*HGKSPATKLLW
GDSDG*VWHENCGGCRKPPQV*IPGGPNGGGAG
GPGDPGPRSGVALRSQQWQVAAVSHGDHRPWQWV
LALPTTWRPYQPSQPHSF*PWGQAIIPTAQAAT
AGSRGRPGPPTCHSGCCQKSQV*DCADLCHRAC
PLCVARVPHP*CPRCHQRDCEGTSVEQHLHRGLQ
RL*PSPGKWLGYPIPPNQHPHHQHGEQDHVVLC
GH*LG
AGGGAAGRNRAVAGAGGRGCRAAAAGAD
HPPAVEVRLLQASPHSRPV*S*EAEGGDEEPAVR
DREADRRLLRGQPPAPGPPG
VTSLSGPAIIRSC
PSTT
QCGSGRRSATHLQGAPCPPRSTG*PAGRL
GTNTTDVLPDPTPSSPSVPFLPIYHKLCL*QSTG
ATTFGW*QQAQAHTPRQEHAHAVWPWGSSHRRA
SAVDLTTPSALHSCALDARGAHCSWTVLVHHGW
CMGSPCLSLCQRQNKPKSLPPEPGGKGPCNVVTP
PPFTLDPS*ATVTGLTLLSKLLTGSSPRAAGWW
APNDTHAREVGILPKVVYGGTWRTWRAQTQQQR
N*KEGPRTACFPASL*SLSPWPQTELAGNAAGRN
KDRQTAT*PGLTVLGHGGILHREEGTNSGQTDV
GRIQRRCHFSLTTTSQPQKAPERPCKTTEGATL
ECRIGRGPCPTPSSQTPR*PCVRGLEVEFLAILG
FQSQPGSAPSPMGCVLRPI*EAEASSRKPLLTP
ACWQAHSPAPAPSMVL*QGNSLPLLVPSLYIGF
SRRPINSSQF

Frame 1

GEQVLTPSSAPSRALAGTPLPLAAMGPGPSRAP
RAPRLMLCALALMVAAGGCVVSAFNLDTRFLVV
KEAGNPGSLFGYSVALHRQTERQQRYLLLAGAPR
ELAVPDGYTNRTGAVYLCPLTAHKDDCERMNIT
VKNDPGHHIIEDMWLGVTVASQGPAGRVLVCAH
RYTQVLWSGSEDQRRMVGKCYVRGNDLELDSSDD
WQTYHNEMCNSNTDYLETGMCQLGTSGGFTQNT
VYFGAPGAYNWKGNSYMIQRKEWDLSEYSYKDP
EDQGNLYIGYTMQVGSFILHPKNITIVTGAPRHR
HMGAVFLLSQEAGGDLRRRQVLEGSQVGAYFGS
AIALADLNNDGWQDLLVGAPYYFERKEEVGGAI
YVFMNQAGTSFPAHPSLLLHGPSGSAFGLSVASI
GDINQDGFQDIAVGAPFEGLGKVYIYHSSSKGL
LRQPQQVIHGEKLGLPGLATFGYSLSGQMDVDE
NFYPDLLVGSLSDHIVLLRARPVINIVHKTLVPR
PAVLDPALCTATSCVQVELCFAYNQSAGNPNYR
RNITLAYTLEADRDRRPPRLRFAGSESAVFHGF
FSMPEMRCQKLELLLMDNLRDKLRPIIISMNYSL
PLRMPDRPRLGLRSLDAYPILNQAQALENHTEV
QFQKECGPDNKCESNLQMRAAFVSEQQQKLSRL
QYSRDVRKLLLSINVTNTRTSERSGEDAHEALLT
LVVPPALLLSSVRPPGACQANETIFCELGNPFK
RNQRMELLIAFEVIGVTLHTRDLQVQLQLSTSS
HQDNLWPMILTLLVDYTLQTSLSMVNHRLQSFFG
GTVMGESGMKTVEDVGSPLKYEFQVGPMGEGLV
GLGTLVLGLEWPYEVSNGKWLLYPTEITVHGNG
SWPCRPPGDLINPLNLTLSDPGDRPSSPQRRRRQ
LDPGGGQGPPPVTLAAAKKAKSETVLTCATGRA
HCVWLECPIPDAPVVTNVTVKARVWNSTFIEDY
RDFDRVRVNGWATLFLRTSIPTINMENKTTWFSV
DIDSE
LVEELPAEIELWLVLVAVGAGLLLLGLI
ILLLWKCGFFKRARTRALYEAKRQKAEMKSQPS
ETERLTDDY*GGSPPPPAHL
V*LL*ADPLLSDHA
QVP
RSADPGGGALPTSREHPAHQEALGDQLADS
GPILLTSSLIPPPPPPVSPFFLFIISYASDSPQ
GPPPLAGSSRLRHIHLVKSMHMLSGPGDLPTGGP
ALWTLQRRVHCIPVP*MHVGPTARGLCWCITDG
AWARRVSASASAKTSQRASHQSREEKAPAMW*H
LPLSHWIHLEPQSLD*LCCQNY*QGAAPGPLAGG
PPMTPMPERWGSCLRLSTGALGGPGVLRPNSKG
TRKKDPERLAFLHLCEASLLGHRLNSQGMQQEG
TKTGKRQRSLGSLCWGTAGSSTERRGPILDRQML
GGYRGDATSHSPLPASLRRPQRDPARPRRERHL
NVE*AGGPAPPHPARPHADHASGA*RWSS*LSL
AFRASLALPPPPWAVS*GPFEKLRLVPENLS*PL
PVGRPTPQPQPLPWYCSRGIPSPSLCLLCI*AS
HGDQ*TAPSL

Frame 2

VNRSSRPAPRPHALSPGPRFRWQPWAPAPAARP
APHA*CSVRSP*WWRPAAASSPPSTWIPDSW**
RRPGTRAASSATRSPSIGRQSGSSATCSWLVPP
GSSLCPMATPTGLVLCTCAHSLPTRMTVSG*TSQ
*KMTLAITLLRTCGLE*LWPARALQAEFWSVPT
ATPRCCGQGQKTSGAWWASATCEAMT*SWTPVM
TGRPTTTRCAIATQTTWRRACASWAPAVASPRTL
CTSAPPVPTTGKETAT*FSARSGTYLSIVTRTQ
RTKETSILGTRCR*AASSCTPKTSPL*QVPHGT
DIWARCSC*ARRQAETCGGGRCWRARRWAPILAA
PLPWQT*TMMGGRTSWWAPPTTSRGKRK*GVPS
MSS*TRREPPSLLTPHSFFMAPVALPLVYLWPA
LVTSTRMDFRILLWELRLKAWAKCTSITVALRGS
LDSPSR*SMERSWDCLGWPPSAIPSVGRWMWMR
TSTQTF*WEACQTTLCCCGPGPSSTSSTRPWCP
GQLCWTLHFARPPLVCKWSCALLTTRVPGTPTTG
ETSPWPTLWRLTGTAGRPGSALPAVSPLSSTAS
SPCPRCAARSWSCS*WTTSVTNSAPSSSP*TTL
YLCGCPIAPGWGCGPWTPTRSSTRHRLWRTTLRS
SSRRSAGLTTSVRATCRCGQPSCQSSSRS*AGS
STAETSGNCS*AST*RTPGPRSAPGRTPTRRCS
PWWCLPPCCCPQCAPPGPAKLMRPSFASWGTPSN
GTRGWSCSSPLRSSG*PCTQGTFRCSCSSPRRV
TRTTCGP*SSLCWWTIHSRPRLAW*ITGYKASL
GGQ*WVSLA*KLWRM*EAPSSMNSRWAQWGRGWW
AWGPWS*VWSGPTKSAMASGCCIPRRSPSMAMG
PGPADHLETLSTLSTSLFLTLGTGHHPHSAGGD
SWIQGEARAPHLSLWLLPKKPSLRLC*PVPQGVP
TVCG*SAPSLMPPLSPT*L*RHECGTAPSSRIT
ETLTESG*MAGLPYSSEPASPPSTWRTRPRGSL
WTLTR
SWWRSCRPKSSCGWCWWPWVQGCCCWG*S
SSCCGSAASSSEPALAPCMKLRGRRRR*RASRQ
RQRG*PTTTEGAAPRPRPTW
CDFFKRTRYYQIM
PKYH
AVRIREEERYPPPGSTLPTKKHWVTSWQTR
DQYY*RPP*SHPLLPQCPLSSYLS*VMPLTVHR
GHHLWLVAAGSGTYTSSRACTCCLALGIFPQEG
QRCGPYNAECTAFLCPRCTWGPLLVDCAGASRMV
HGLAVSQPLPAPKQAKEPPTRAGRKRPLQCGDT
SPFHTGSILSHSHWIDFAVKTTDREQPPGRWLV
GPQ*HPCQRGGDPA*GCLRGHLEDLACSDPTAKE
LERRTQNGLLSCISVKPLSLATD*TRRECSRKE
QRQANGNVAWAHCAGARRDPPQRGGDQFWTDRC
WEDTEEMPLLTHHYQPASEGPRETLQDHGGSDT*
M*NRQGALPHPIQPDPTLTMRQGPRGGVLSYPW
LSEPAWLCPLPHGLCPKAHLRS*G*FQKTSPDP
CLLAGPLPSPSPFHGTVAGEFPPPPCAFFVYRLL
TATNKQLPVC

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