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Sequences

Sequence NM_015356

CCATGCTCAAGTGCATCCCGCTGTGGCGCTGCAACCGGCACGTGGAGTCGGTGGACAAGCGGCACTGTTCGCTGCAGGCCGTGCCGGAGGAGATCTACCG CTACAGCCGCAGCCTGGAGGAGCTGCTGCTCGACGCCAACCAGCTGCGCGAGCTGCCCAAGCCTTTTTTCCGGCTGCTGAACTTGCGCAAGCTGGGCCTG AGCGACAACGAGATCCAGCGGTTGCCTCCCGAGGTGGCCAACTTCATGCAGCTGGTGGAGCTGGACGTGTCCCGGAACGATATCCCTGAGATCCCGGAGA GCATCAAGTTCTGCAAGGCTCTGGAGATCGCGGACTTCAGCGGGAACCCCCTCTCCAGGCTCCCTGATGGCTTCACTCAGCTGCGCAGCCTGGCTCACCT GGCCCTGAATGATGTGTCTCTGCAGGCACTGCCCGGGGACGTGGGCAACCTCGCCAACCTGGTGACCCTGGAGCTCCGGGAGAACCTGCTCAAGTCCCTG CCAGCGTCCCTGTCATTTCTGGTCAAGCTGGAACAGCTGGATCTGGGAGGCAACGATCTGGAAGTGCTGCCAGACACTCTGGGGGCTCTGCCCAATCTTC GGGAGCTGTGGCTTGACCGGAACCAGCTGTCAGCACTGCCCCCGGAGCTCGGGAACCTGCGGCGCCTGGTGTGCCTGGACGTGTCGGAAAACCGGCTGGA GGAGCTGCCTGCTGAGCTCGGCGGGCTGGTGCTGCTCACTGACCTGCTGCTGTCCCAGAACCTGCTGCGGAGGCTGCCCGACGGCATCGGTCAGCTGAAG CAGCTATCCATCCTAAAGGTAGACCAGAATCGGCTGTGCGAGGTGACCGAGGCCATCGGGGACTGTGAGAACCTCTCTGAGCTGATCCTCACGGAGAACC TGCTGATGGCCCTGCCCCGCTCCCTGGGAAAGCTGACTAAGCTGACCAACCTCAACGTGGACCGGAACCACCTCGAGGCGCTGCCGCCCGAGATCGGGGG CTGTGTGGCACTCAGCGTCCTCTCCTTGAGGGACAACCGCCTGGCCGTCCTGCCACCAGAGCTGGCCCACACGACAGAGCTGCACGTGCTGGACGTGGCG GGGAACCGCCTGCAGAGTCTGCCGTTCGCGCTCACCCACCTCAATCTCAAGGCCCTGTGGCTGGCAGAGAACCAGGCGCAGCCCATGCTCCGGTTCCAGA CGGAGGATGATGCCCGGACCGGCGAGAAGGTGCTCACCTGCTACTTGCTGCCCCAGCAGCCCCCACCCAGCCTCGAGGATGCTGGGCAGCAGGGGAGCCT CTCGGAGACCTGGAGCGATGCCCCGCCGAGCCGCGTCAGCGTCATCCAGTTCCTGGAGGCCCCCATAGGTGATGAGGACGCTGAGGAAGCTGCAGCTGAG AAGCGGGGCCTACAGCGCCGGGCCACACCTCACCCCAGCGAGCTCAAGGTGATGAAGAGGAGCATCGAGGGGCGGCGGAGCGAGGCCTGCCCTTGCCAGC CAGACTCTGGGTCGCCCTTGCCTGCAGAGGAGGAGAAGCGGCTGAGTGCCGAGTCTGGCCTGAGTGAAGACTCTCGCCCATCTGCCAGCACAGTCTCTGA GGCTGAGCCCGAGGGCCCGTCGGCTGAGGCACAGGGTGGGAGCCAGCAGGAAGCCACGACTGCTGGCGGGGAGGAAGACGCCGAAGAGGACTACCAGGAG CCCACGGTGCATTTCGCAGAGGACGCACTGCTGCCCGGGGATGACAGGGAGATCGAGGAGGGGCAGCCTGAGGCCCCCTGGACCCTGCCAGGCGGGAGGC AGCGGCTCATCCGCAAGGACACACCTCACTACAAAAAGCACTTCAAGATCTCCAAGCTGCCCCAGCCCGAGGCCGTTGTGGCTCTGCTGCAGGGCATGCA GCCTGATGGGGAGGGCCCTGTGGCTCCCGGGGGCTGGCACAATGGCCCCCACGCACCCTGGGCTCCTCGGGCCCAGAAGGAGGAGGAGGAGGAGGAAGAG GGTAGTCCTCAGGAGGAGGAGGTAGAGGAGGAGGAGGAAAACAGGGCTGAAGAGGAAGAGGCCAGCACTGAGGAGGAGGACAAGGAGGGGGCCGTGGTTT CTGCGCCCTCTGTCAAGGGAGTGTCGTTTGACCAGGCCAATAACCTGCTGATAGAGCCTGCTCGCATTGAGGAGGAAGAGCTGACCCTCACTATCCTGCG GCAGACTGGGGGCCTGGGCATCAGCATTGCGGGCGGCAAGGGCTCCACACCCTATAAGGGGGACGACGAGGGCATATTCATCTCTCGGGTGTCCGAGGAA GGCCCTGCGGCCCGGGCTGGAGTCCGTGTGGGTGACAAGCTCCTGGAGGTGAATGGTGTGGCTCTGCAGGGCGCCGAGCACCACGAGGCCGTGGAGGCGC TCCGGGGGGCCGGCACTGCCGTGCAGATGCGAGTGTGGCGGGAGCGCATGGTGGAGCCTGAGAACGCGGTCACCATCACGCCGCTGCGGCCCGAGGATGA TTACAGCCCCCGAGAGCGGCGGGGAGGGGGGCTGCGCCTGCCCCTGCTCCCGCCTGAGAGCCCCGGGCCCCTCCGTCAGCGCCACGTGGCCTGCCTGGCA CGCAGCGAGAGGGGGCTGGGCTTCAGCATTGCTGGTGGGAAAGGCTCCACACCCTACAGGGCTGGTGATGCGGGCATCTTCGTCTCCCGCATTGCCGAGG GCGGTGCTGCTCACCGCGCGGGCACACTGCAGGTTGGCGACCGCGTCCTCTCTATTAATGGAGTGGACGTGACTGAGGCCAGGCATGACCACGCCGTCTC CCTGCTGACCGCTGCCTCCCCCACCATCGCCCTGCTGTTGGAGCGGGAGGCTGGGGGCCCTCTTCCTCCCAGCCCTCTGCCACATTCCTCACCCCCCACC GCTGCTGTTGCCACCACCAGCATAACCACTGCCACCCCCGGGGTGCCTGGGTTGCCGAGCCTGGCCCCCAGCCTGCTGGCTGCCGCGTTGGAAGGGCCAT ACCCAGTGGAGGAGATCCGTCTGCCAAGAGCTGGGGGCCCTCTGGGGCTTAGTATTGTCGGAGGCTCCGACCATTCCAGCCACCCGTTTGGTGTCCAGGA GCCTGGTGTGTTCATCTCCAAGGTGCTCCCGCGGGGCCTGGCCGCTCGCAGCGGCCTGCGGGTTGGGGACCGCATCCTGGCAGTGAACGGGCAAGACGTG CGGGATGCCACGCACCAAGAAGCAGTCAGTGCCCTGCTCCGGCCCTGCCTGGAGCTGTCGCTGCTGGTGCGGAGGGACCCGGCACCCCCGGGCCTACGGG AACTGTGCATCCAGAAGGCACCTGGGGAGAGGCTGGGCATCAGCATCCGCGGGGGTGCCAGGGGCCACGCTGGCAACCCCCGCGACCCCACAGACGAGGG CATCTTCATCTCCAAGGTGAGCCCCACGGGGGCAGCCGGGCGCGACGGTCGGCTGCGTGTGGGTTTGCGGCTGTTGGAGGTGAACCAGCAGAGCCTGCTG GGCCTGACGCACGGCGAGGCGGTGCAGCTGCTCCGCAGTGTGGGCGACACCCTCACCGTGCTGGTCTGTGACGGCTTTGAGGCCAGCACCGACGCAGCCC TGGAGGTGTCCCCAGGTGTCATTGCCAACCCCTTTGCGGCAGGCATCGGCCACCGGAACAGCCTGGAGAGCATCTCTTCCATCGACCGGGAGCTGAGCCC TGAGGGCCCAGGCAAGGAGAAGGAGCTGCCTGGACAGACCCTGCACTGGGGGCCCGAGGCCACAGAAGCCGCAGGTCGGGGTCTGCAGCCCCTGAAGCTG GACTACCGCGCCCTGGCCGCCGTGCCCAGCGCTGGCAGCGTGCAGAGGGTACCGTCTGGAGCAGCTGGAGGGAAGATGGCTGAATCTCCCTGCTCCCCTA GTGGCCAGCAGCCGCCCTCCCCGCCTTCTCCGGATGAGCTGCCCGCCAATGTGAAGCAGGCCTACAGGGCCTTCGCGGCCGTGCCCACTTCTCACCCGCC TGAGGATGCCCCTGCCCAGCCCCCCACGCCTGGGCCTGCAGCCTCCCCGGAGCAGCTGTCCTTCCGGGAGCGGCAGAAGTACTTTGAGCTGGAGGTGCGC GTGCCCCAGGCCGAGGGCCCCCCTAAGCGCGTGTCCCTGGTGGGTGCTGACGACCTGCGGAAGATGCAGGAGGAGGAAGCCAGAAAACTACAGCAGAAGA GAGCGCAGATGCTGCGGGAGGCGGCAGAGGCTGGGGCCGAAGCGAGGCTCGCCCTGGACGGGGAGACGCTGGGCGAGGAGGAACAGGAGGATGAGCAGCC ACCCTGGGCCAGCCCGAGCCCCACCTCAAGGCAGAGCCCGGCGTCCCCCCCACCCCTGGGAGGTGGCGCCCCGGTGCGGACGGCCAAAGCTGAACGGCGC CACCAGGAGCGGCTGCGCGTGCAGAGTCCGGAGCCACCGGCACCCGAGCGTGCCCTGTCCCCTGCCGAGCTCCGGGCCCTGGAGGCCGAGAAGCGTGCGC TGTGGAGGGCAGCCAGGATGAAGTCATTGGAACAGGACGCTCTCCGAGCACAGATGGTCCTCAGCAGGTCCCAGGAAGGCCGGGGCACGCGGGGGCCCCT GGAGCGACTGGCCGAGGCCCCTTCCCCTGCGCCCACCCCGTCGCCCACCCCTGTGGAAGACCTCGGCCCCCAGACCAGCACCTCCCCGGGACGCCTGTCA CCGGACTTTGCTGAGGAGTTGAGGTCCCTGGAACCATCTCCCAGCCCTGGCCCGCAGGAGGAGGATGGAGAAGTGGCTCTGGTGCTTCTGGGCAGGCCCT CACCCGGCGCTGTGGGCCCTGAAGATGTGGCACTGTGCAGCAGCCGCCGCCCCGTAAGGCCTGGGCGCCGTGGCCTGGGCCCTGTGCCCTCCTAGAGGAG CAGGCACCTCCCCCAGACTTGGGGTGGGGGCCCTGCCAGCTCCAGCACCACCCTTGCCCCAAGTCTTTTAACCTGGGTGTTAGCATTTTAAAGAGACCCC ACAGGAGTTCTGGCCTGTGACTAACTAACTGCCCCACCCCAGCCGAGACCTCGGCGAGACTGTAACTAGTGATGTTTGTACAACCAAAGACTCTATTTTG TGGTTTAAGGAGAATAAAGTTGACTACATTTTA

refseq_SCRIB.F1 (forward primer)
refseq_SCRIB.R1 (reverse primer)
Single exon skipping

Frame 0

PCSSASRCGAATGTWSRWTSGTVRCRPCRRRST ATAAAWRSCCSTPTSCASCPSLFSGC*TCASWA* ATTRSSGCLPRWPTSCSWWSWTCPGTISLRSRR ASSSARLWRSRTSAGTPSPGSLMASLSCAAWLT WP*MMCLCRHCPGTWATSPTW*PWSSGRTCSSPC QRPCHFWSSWNSWIWEATIWKCCQTLWGLCPIF GSCGLTGTSCQHCPRSSGTCGAWCAWTCRKTGW RSCLLSSAGWCCSLTCCCPRTCCGGCPTASVS*S SYPS*R*TRIGCAR*PRPSGTVRTSLS*SSRRT C*WPCPAPWES*LS*PTSTWTGTTSRRCRPRSG AVWHSASSP*GTTAWPSCHQSWPTRQSCTCWTWR GTACRVCRSRSPTSISRPCGWQRTRRSPCSGSR RRMMPGPARRCSPATCCPSSPHPASRMLGSRGA SRRPGAMPRRAASASSSSWRPP*VMRTLRKLQLR SGAYSAGPHLTPASSR**RGASRGGGARPALAS QTLGRPCLQRRRSG*VPSLA*VKTLAHLPAQSL RLSPRARRLRHRVGASRKPRLLAGRKTPKRTTRS PRCISQRTHCCPGMTGRSRRGSLRPPGPCQAGG SGSSARTHLTTKSTSRSPSCPSPRPLWLCCRAC SLMGRALWLPGAGTMAPTHPGLLGPRRRRRRRKR VVLRRRR*RRRRKTGLKRKRPALRRRTRRGPWF LRPLSRECRLTRPITC**SLLALRRKS*PSLSC GRLGAWASALRAARAPHPIRGTTRAYSSLGCPRK ALRPGLESVWVTSSWR*MVWLCRAPSTTRPWRR SGGPALPCRCECGGSAWWSLRTRSPSRRCGPRM ITAPESGGEGGCACPCSRLRAPGPSVSATWPAWH AARGGWASALLVGKAPHPTGLVMRASSSPALPR AVLLTARAHCRLATASSLLMEWT*LRPGMTTPS PC*PLPPPPSPCCWSGRLGALFLPALCHIPHPPP LLLPPPA*PLPPPGCLGCRAWPPACWLPRWKGH TQWRRSVCQELGALWGLVLSEAPTIPATRLVSR SLVCSSPRCSRGAWPLAAACGLGTASWQ*TGKTC GMPRTKKQSVPCSGPAWSCRCWCGGTRHPRAYG NCASRRHLGRGWASASAGVPGATLATPATPQTR ASSSPR*APRGQPGATVGCVWVCGCWR*TSRACW A*RTARRCSCSAVWATPSPCWSVTALRPAPTQP WRCPQVSLPTPLRQASATGTAWRASLPSTGS*A LRAQARRRSCLDRPCTGGPRPQKPQVGVCSP*SW TTAPWPPCPALAACRGYRLEQLEGRWLNLPAPL VASSRPPRLLRMSCPPM*SRPTGPSRPCPLLTR LRMPLPSPPRLGLQPPRSSCPSGSGRSTLSWRCA CPRPRAPLSACPWWVLTTCGRCRRRKPENYSRR ERRCCGRRQRLGPKRGSPWTGRRWARRNRRMSS HPGPARAPPQGRARRPPHPWEVAPRCGRPKLNGA TRSGCACRVRSHRHPSVPCPLPSSGPWRPRSVR CGGQPG*SHWNRTLSEHRWSSAGPRKAGARGGP WSDWPRPLPLRPPRRPPLWKTSAPRPAPPRDACH RTLLRS*GPWNHLPALARRRRMEKWLWCFWAGP HPALWALKMWHCAAAAAP*GLGAVAWALCPPRG AGTSPRLGVGALPAPAPPLPQVF*PGC*HFKETP QEFWPVTN*LPHPSRDLGETVTSDVCTTKDSIL WFKENKVDYIL

Frame 1

HAQVHPAVALQPARGVGGQAALFAAGRAGGDLP LQPQPGGAAARRQPAARAAQAFFPAAELAQAGP ERQRDPAVASRGGQLHAAGGAGRVPERYP*DPGE HQVLQGSGDRGLQREPPLQAP*WLHSAAQPGSP GPE*CVSAGTARGRGQPRQPGDPGAPGEPAQVP ASVPVISGQAGTAGSGRQRSGSAARHSGGSAQSS GAVA*PEPAVSTAPGAREPAAPGVPGRVGKPAG GAAC*ARRAGAAH*PAAVPEPAAEAARRHRSAE AAIHPKGRPESAVRGDRGHRGL*EPL*ADPHGEP ADGPAPLPGKAD*ADQPQRGPEPPRGAAARDRG LCGTQRPLLEGQPPGRPATRAGPHDRAARAGRG GEPPAESAVRAHPPQSQGPVAGREPGAAHAPVPD GG*CPDRREGAHLLLAAPAAPTQPRGCWAAGEP LGDLERCPAEPRQRHPVPGGPHR**GR*GSCS* EAGPTAPGHTSPQRAQGDEEEHRGAAERGLPLPA RLWVALACRGGEAAECRVWPE*RLSPICQHSL* G*ARGPVG*GTGWEPAGSHDCWRGGRRRRGLPG AHGAFRRGRTAARG*QGDRGGAA*GPLDPARREA AAHPQGHTSLQKALQDLQAAPARGRCGSAAGHA A*WGGPCGSRGLAQWPPRTLGSSGPEGGGGGGR G*SSGGGGRGGGGKQG*RGRGQH*GGGQGGGRGF CALCQGSVV*PGQ*PADRACSH*GGRADPHYPA ADWGPGHQHCGRQGLHTL*GGRRGHIHLSGVRG RPCGPGWSPCG*QAPGGEWCGSAGRRAPRGRGGA PGGRHCRADASVAGAHGGA*ERGHHHAAAARG* LQPPRAAGRGAAPAPAPA*EPRAPPSAPRGLPG TQREGAGLQHCWWERLHTLQGW*CGHLRLPHCRG RCCSPRGHTAGWRPRPLY*WSGRD*GQA*PRRL PADRCLPHHRPAVGAGGWGPSSSQPSATFLTPH RCCCHHQHNHCHPRGAWVAEPGPQPAGCRVGRAI PSGGDPSAKSWGPSGA*YCRRLRPFQPPVWCPG AWCVHLQGAPAGPGRSQRPAGWGPHPGSERARR AGCHAPRSSQCPAPALPGAVAAGAEGPGTPGPTG TVHPEGTWGEAGHQHPRGCQGPRWQPPRPHRRG HLHLQGEPHGGSRARRSAACGFAAVGGEPAEPA GPDARRGGAAAPQCGRHPHRAGL*RL*GQHRRSP GGVPRCHCQPLCGRHRPPEQPGEHLFHRPGAEP *GPRQGEGAAWTDPALGARGHRSRRSGSAAPEA GLPRPGRRAQRWQRAEGTVWSSWREDG*ISLLP* WPAAALPAFSG*AARQCEAGLQGLRGRAHFSPA *GCPCPAPHAWACSLPGAAVLPGAAEVL*AGGA RAPGRGPP*ARVPGGC*RPAEDAGGGSQKTTAEE SADAAGGGRGWGRSEARPGRGDAGRGGTGG*AA TLGQPEPHLKAEPGVPPTPGRWRPGADGQS*TA PPGAAARAESGATGTRACPVPCRAPGPGGREACA VEGSQDEVIGTGRSPSTDGPQQVPGRPGHAGAP GATGRGPFPCAHPVAHPCGRPRPPDQHLPGTPV TGLC*GVEVPGTISQPWPAGGGWRSGSGASGQAL TRRCGP*RCGTVQQPPPRKAWAPWPGPCALLEE QAPPPDLGWGPCQLQHHPCPKSFNLGVSILKRP HRSSGL*LTNCPTPAETSARL*LVMFVQPKTLFC GLRRIKLTTF

Frame 2

MLKCIPLWRCNRHVESVDKRHCSLQAVPEEIYR YSRSLEELLLDANQLRELPKPFFRLLNLRKLGL SDNEIQRLPPEVANFMQLVELDVSRNDIPEIPE SIKFCKALEIADFSGNPLSRLPDGFTQLRSLAHL ALNDVSLQALPGDVGNLANLVTLELRENLLKSL PASLSFLVKLEQLDLGGNDLEVLPDTLGALPNL RELWLDRNQLSALPPELGNLRRLVCLDVSENRLE ELPAELGGLVLLTDLLLSQNLLRRLPDGIGQLK QLSILKVDQNRLCEVTEAIGDCENLSELILTEN LLMALPRSLGKLTKLTNLNVDRNHLEALPPEIGG CVALSVLSLRDNRLAVLPPELAHTTELHVLDVA GNRLQSLPFALTHLNLKALWLAENQAQPMLRFQ TEDDARTGEKVLTCYLLPQQPPPSLEDAGQQGSL SETWSDAPPSRVSVIQFLEAPIGDEDAEEAAAE KRGLQRRATPHPSELKVMKRSIEGRRSEACPCQ PDSGSPLPAEEEKRLSAESGLSEDSRPSASTVSE AEPEGPSAEAQGGSQQEATTAGGEEDAEEDYQE PTVHFAEDALLPGDDREIEEGQPEAPWTLPGGR QRLIRKDTPHYKKHFKISKLPQPEAVVALLQGMQ PDGEGPVAPGGWHNGPHAPWAPRAQKEEEEEEE GSPQEEEVEEEEENRAEEEEASTEEEDKEGAVV SAPSVKGVSFDQANNLLIEPARIEEEELTLTILR QTGGLGISIAGGKGSTPYKGDDEGIFISRVSEE GPAARAGVRVGDKLLEVNGVALQGAEHHEAVEA LRGAGTAVQMRVWRERMVEPENAVTITPLRPEDD YSPRERRGGGLRLPLLPPESPGPLRQRHVACLA RSERGLGFSIAGGKGSTPYRAGDAGIFVSRIAE GGAAHRAGTLQVGDRVLSINGVDVTEARHDHAVS LLTAASPTIALLLEREAGGPLPPSPLPHSSPPT AAVATTSITTATPGVPGLPSLAPSLLAAALEGP YPVEEIRLPRAGGPLGLSIVGGSDHSSHPFGVQE PGVFISKVLPRGLAARSGLRVGDRILAVNGQDV RDATHQEAVSALLRPCLELSLLVRRDPAPPGLR ELCIQKAPGERLGISIRGGARGHAGNPRDPTDEG IFISKVSPTGAAGRDGRLRVGLRLLEVNQQSLL GLTHGEAVQLLRSVGDTLTVLVCDGFEASTDAA LEVSPGVIANPFAAGIGHRNSLESISSIDRELSP EGPGKEKELPGQTLHWGPEATEAAGRGLQPLKL DYRALAAVPSAGSVQRVPSGAAGGKMAESPCSP SGQQPPSPPSPDELPANVKQAYRAFAAVPTSHPP EDAPAQPPTPGPAASPEQLSFRERQKYFELEVR VPQAEGPPKRVSLVGADDLRKMQEEEARKLQQK RAQMLREAAEAGAEARLALDGETLGEEEQEDEQP PWASPSPTSRQSPASPPPLGGGAPVRTAKAERR HQERLRVQSPEPPAPERALSPAELRALEAEKRA LWRAARMKSLEQDALRAQMVLSRSQEGRGTRGPL ERLAEAPSPAPTPSPTPVEDLGPQTSTSPGRLS PDFAEELRSLEPSPSPGPQEEDGEVALVLLGRP SPGAVGPEDVALCSSRRPVRPGRRGLGPVPS*RS RHLPQTWGGGPASSSTTLAPSLLTWVLAF*RDP TGVLACD*LTAPPQPRPRRDCN**CLYNQRLYF VV*GE*S*LHF

Sequence NM_182706

CCATGCTCAAGTGCATCCCGCTGTGGCGCTGCAACCGGCACGTGGAGTCGGTGGACAAGCGGCACTGTTCGCTGCAGGCCGTGCCGGAGGAGATCTACCG CTACAGCCGCAGCCTGGAGGAGCTGCTGCTCGACGCCAACCAGCTGCGCGAGCTGCCCAAGCCTTTTTTCCGGCTGCTGAACTTGCGCAAGCTGGGCCTG AGCGACAACGAGATCCAGCGGTTGCCTCCCGAGGTGGCCAACTTCATGCAGCTGGTGGAGCTGGACGTGTCCCGGAACGATATCCCTGAGATCCCGGAGA GCATCAAGTTCTGCAAGGCTCTGGAGATCGCGGACTTCAGCGGGAACCCCCTCTCCAGGCTCCCTGATGGCTTCACTCAGCTGCGCAGCCTGGCTCACCT GGCCCTGAATGATGTGTCTCTGCAGGCACTGCCCGGGGACGTGGGCAACCTCGCCAACCTGGTGACCCTGGAGCTCCGGGAGAACCTGCTCAAGTCCCTG CCAGCGTCCCTGTCATTTCTGGTCAAGCTGGAACAGCTGGATCTGGGAGGCAACGATCTGGAAGTGCTGCCAGACACTCTGGGGGCTCTGCCCAATCTTC GGGAGCTGTGGCTTGACCGGAACCAGCTGTCAGCACTGCCCCCGGAGCTCGGGAACCTGCGGCGCCTGGTGTGCCTGGACGTGTCGGAAAACCGGCTGGA GGAGCTGCCTGCTGAGCTCGGCGGGCTGGTGCTGCTCACTGACCTGCTGCTGTCCCAGAACCTGCTGCGGAGGCTGCCCGACGGCATCGGTCAGCTGAAG CAGCTATCCATCCTAAAGGTAGACCAGAATCGGCTGTGCGAGGTGACCGAGGCCATCGGGGACTGTGAGAACCTCTCTGAGCTGATCCTCACGGAGAACC TGCTGATGGCCCTGCCCCGCTCCCTGGGAAAGCTGACTAAGCTGACCAACCTCAACGTGGACCGGAACCACCTCGAGGCGCTGCCGCCCGAGATCGGGGG CTGTGTGGCACTCAGCGTCCTCTCCTTGAGGGACAACCGCCTGGCCGTCCTGCCACCAGAGCTGGCCCACACGACAGAGCTGCACGTGCTGGACGTGGCG GGGAACCGCCTGCAGAGTCTGCCGTTCGCGCTCACCCACCTCAATCTCAAGGCCCTGTGGCTGGCAGAGAACCAGGCGCAGCCCATGCTCCGGTTCCAGA CGGAGGATGATGCCCGGACCGGCGAGAAGGTGCTCACCTGCTACTTGCTGCCCCAGCAGCCCCCACCCAGCCTCGAGGATGCTGGGCAGCAGGGGAGCCT CTCGGAGACCTGGAGCGATGCCCCGCCGAGCCGCGTCAGCGTCATCCAGTTCCTGGAGGCCCCCATAGGTGATGAGGACGCTGAGGAAGCTGCAGCTGAG AAGCGGGGCCTACAGCGCCGGGCCACACCTCACCCCAGCGAGCTCAAGGTGATGAAGAGGAGCATCGAGGGGCGGCGGAGCGAGGCCTGCCCTTGCCAGC CAGACTCTGGGTCGCCCTTGCCTGCAGAGGAGGAGAAGCGGCTGAGTGCCGAGTCTGGCCTGAGTGAAGACTCTCGCCCATCTGCCAGCACAGTCTCTGA GGCTGAGCCCGAGGGCCCGTCGGCTGAGGCACAGGGTGGGAGCCAGCAGGAAGCCACGACTGCTGGCGGGGAGGAAGACGCCGAAGAGGACTACCAGGAG CCCACGGTGCATTTCGCAGAGGACGCACTGCTGCCCGGGGATGACAGGGAGATCGAGGAGGGGCAGCCTGAGGCCCCCTGGACCCTGCCAGGCGGGAGGC AGCGGCTCATCCGCAAGGACACACCTCACTACAAAAAGCACTTCAAGATCTCCAAGCTGCCCCAGCCCGAGGCCGTTGTGGCTCTGCTGCAGGGCATGCA GCCTGATGGGGAGGGCCCTGTGGCTCCCGGGGGCTGGCACAATGGCCCCCACGCACCCTGGGCTCCTCGGGCCCAGAAGGAGGAGGAGGAGGAGGAAGAG GGTAGTCCTCAGGAGGAGGAGGTAGAGGAGGAGGAGGAAAACAGGGCTGAAGAGGAAGAGGCCAGCACTGAGGAGGAGGACAAGGAGGGGGCCGTGGTTT CTGCGCCCTCTGTCAAGGGAGTGTCGTTTGACCAGGCCAATAACCTGCTGATAGAGCCTGCTCGCATTGAGGAGGAAGAGCTGACCCTCACTATCCTGCG GCAGACTGGGGGCCTGGGCATCAGCATTGCGGGCGGCAAGGGCTCCACACCCTATAAGGGGGACGACGAGGGCATATTCATCTCTCGGGTGTCCGAGGAA GGCCCTGCGGCCCGGGCTGGAGTCCGTGTGGGTGACAAGCTCCTGGAGGTGAATGGTGTGGCTCTGCAGGGCGCCGAGCACCACGAGGCCGTGGAGGCGC TCCGGGGGGCCGGCACTGCCGTGCAGATGCGAGTGTGGCGGGAGCGCATGGTGGAGCCTGAGAACGCGGTCACCATCACGCCGCTGCGGCCCGAGGATGA TTACAGCCCCCGAGAGCGGCGGGGAGGGGGGCTGCGCCTGCCCCTGCTCCCGCCTGAGAGCCCCGGGCCCCTCCGTCAGCGCCACGTGGCCTGCCTGGCA CGCAGCGAGAGGGGGCTGGGCTTCAGCATTGCTGGTGGGAAAGGCTCCACACCCTACAGGGCTGGTGATGCGGGCATCTTCGTCTCCCGCATTGCCGAGG GCGGTGCTGCTCACCGCGCGGGCACACTGCAGGTTGGCGACCGCGTCCTCTCTATTAATGGAGTGGACGTGACTGAGGCCAGGCATGACCACGCCGTCTC CCTGCTGACCGCTGCCTCCCCCACCATCGCCCTGCTGTTGGAGCGGGAGGCTGGGGGCCCTCTTCCTCCCAGCCCTCTGCCACATTCCTCACCCCCCACC GCTGCTGTTGCCACCACCAGCATAACCACTGCCACCCCCGGGGTGCCTGGGTTGCCGAGCCTGGCCCCCAGCCTGCTGGCTGCCGCGTTGGAAGGGCCAT ACCCAGTGGAGGAGATCCGTCTGCCAAGAGCTGGGGGCCCTCTGGGGCTTAGTATTGTCGGAGGCTCCGACCATTCCAGCCACCCGTTTGGTGTCCAGGA GCCTGGTGTGTTCATCTCCAAGGTGCTCCCGCGGGGCCTGGCCGCTCGCAGCGGCCTGCGGGTTGGGGACCGCATCCTGGCAGTGAACGGGCAAGACGTG CGGGATGCCACGCACCAAGAAGCAGTCAGTGCCCTGCTCCGGCCCTGCCTGGAGCTGTCGCTGCTGGTGCGGAGGGACCCGGCACCCCCGGGCCTACGGG AACTGTGCATCCAGAAGGCACCTGGGGAGAGGCTGGGCATCAGCATCCGCGGGGGTGCCAGGGGCCACGCTGGCAACCCCCGCGACCCCACAGACGAGGG CATCTTCATCTCCAAGGTGAGCCCCACGGGGGCAGCCGGGCGCGACGGTCGGCTGCGTGTGGGTTTGCGGCTGTTGGAGGTGAACCAGCAGAGCCTGCTG GGCCTGACGCACGGCGAGGCGGTGCAGCTGCTCCGCAGTGTGGGCGACACCCTCACCGTGCTGGTCTGTGACGGCTTTGAGGCCAGCACCGACGCAGCCC TGGAGGTGTCCCCAGGTGTCATTGCCAACCCCTTTGCGGCAGGCATCGGCCACCGGAACAGCCTGGAGAGCATCTCTTCCATCGACCGGGAGCTGAGCCC TGAGGGCCCAGGCAAGGAGAAGGAGCTGCCTGGACAGACCCTGCACTGGGGGCCCGAGGCCACAGAAGCCGCAGGTCGGGGTCTGCAGCCCCTGAAGCTG GACTACCGCGCCCTGGCCGCCGTGCCCAGCGCTGGCAGCGTGCAGAGGGTACCGTCTGGAGCAGCTGGAGGGAAGATGGCTGAATCTCCCTGCTCCCCTA GTGGCCAGCAGCCGCCCTCCCCGCCTTCTCCGGATGAGCTGCCCGCCAATGTGAAGCAGGCCTACAGGGCCTTCGCGGCCGTGCCCACTTCTCACCCGCC TGAGGATGCCCCTGCCCAGCCCCCCACGCCTGGGCCTGCAGCCTCCCCGGAGCAGCTGTCCTTCCGGGAGCGGCAGAAGTACTTTGAGCTGGAGGTGCGC GTGCCCCAGGCCGAGGGCCCCCCTAAGCGCGTGTCCCTGGTGGGTGCTGACGACCTGCGGAAGATGCAGGAGGAGGAAGCCAGAAAACTACAGCAGAAGA GAGCGCAGATGCTGCGGGAGGCGGCAGAGGCTGGGGCCGAAGCGAGGCTCGCCCTGGACGGGGAGACGCTGGGCGAGGAGGAACAGGAGGATGAGCAGCC ACCCTGGGCCAGCCCGAGCCCCACCTCAAGGCAGAGCCCGGCGTCCCCCCCACCCCTGGGAGGTGGCGCCCCGGTGCGGACGGCCAAAGCTGAACGGCGC CACCAGGAGCGGCTGCGCGTGCAGAGTCCGGAGCCACCGGCACCCGAGCGTGCCCTGTCCCCTGCCGAGCTCCGGGCCCTGGAGGCCGAGAAGCGTGCGC TGTGGAGGGCAGCCAGGATGAAGTCATTGGAACAGGACGCTCTCCGAGCACAGATGGTCCTCAGCAGGTCCCAGGAAGGCCGGGGCACGCGGGGGCCCCT GGAGCGACTGGCCGAGGCCCCTTCCCCTGCGCCCACCCCGTCGCCCACCCCTGTGGAAGACCTCGGCCCCCAGACCAGCACCTCCCCGGGACGCCTGCCC TTGTCTGGAAAGAAGTTTGACTACAGGGCCTTTGCGGCCCTGCCTTCTTCCAGACCTGTCTATGACATCCAGTCACCGGACTTTGCTGAGGAGTTGAGGT CCCTGGAACCATCTCCCAGCCCTGGCCCGCAGGAGGAGGATGGAGAAGTGGCTCTGGTGCTTCTGGGCAGGCCCTCACCCGGCGCTGTGGGCCCTGAAGA TGTGGCACTGTGCAGCAGCCGCCGCCCCGTAAGGCCTGGGCGCCGTGGCCTGGGCCCTGTGCCCTCCTAGAGGAGCAGGCACCTCCCCCAGACTTGGGGT GGGGGCCCTGCCAGCTCCAGCACCACCCTTGCCCCAAGTCTTTTAACCTGGGTGTTAGCATTTTAAAGAGACCCCACAGGAGTTCTGGCCTGTGACTAAC TAACTGCCCCACCCCAGCCGAGACCTCGGCGAGACTGTAACTAGTGATGTTTGTACAACCAAAGACTCTATTTTGTGGTTTAAGGAGAATAAAGTTGACT ACATTTTA

refseq_SCRIB.F1 (forward primer)
refseq_SCRIB.R1 (reverse primer)
Single exon skipping

Frame 0

PCSSASRCGAATGTWSRWTSGTVRCRPCRRRST ATAAAWRSCCSTPTSCASCPSLFSGC*TCASWA* ATTRSSGCLPRWPTSCSWWSWTCPGTISLRSRR ASSSARLWRSRTSAGTPSPGSLMASLSCAAWLT WP*MMCLCRHCPGTWATSPTW*PWSSGRTCSSPC QRPCHFWSSWNSWIWEATIWKCCQTLWGLCPIF GSCGLTGTSCQHCPRSSGTCGAWCAWTCRKTGW RSCLLSSAGWCCSLTCCCPRTCCGGCPTASVS*S SYPS*R*TRIGCAR*PRPSGTVRTSLS*SSRRT C*WPCPAPWES*LS*PTSTWTGTTSRRCRPRSG AVWHSASSP*GTTAWPSCHQSWPTRQSCTCWTWR GTACRVCRSRSPTSISRPCGWQRTRRSPCSGSR RRMMPGPARRCSPATCCPSSPHPASRMLGSRGA SRRPGAMPRRAASASSSSWRPP*VMRTLRKLQLR SGAYSAGPHLTPASSR**RGASRGGGARPALAS QTLGRPCLQRRRSG*VPSLA*VKTLAHLPAQSL RLSPRARRLRHRVGASRKPRLLAGRKTPKRTTRS PRCISQRTHCCPGMTGRSRRGSLRPPGPCQAGG SGSSARTHLTTKSTSRSPSCPSPRPLWLCCRAC SLMGRALWLPGAGTMAPTHPGLLGPRRRRRRRKR VVLRRRR*RRRRKTGLKRKRPALRRRTRRGPWF LRPLSRECRLTRPITC**SLLALRRKS*PSLSC GRLGAWASALRAARAPHPIRGTTRAYSSLGCPRK ALRPGLESVWVTSSWR*MVWLCRAPSTTRPWRR SGGPALPCRCECGGSAWWSLRTRSPSRRCGPRM ITAPESGGEGGCACPCSRLRAPGPSVSATWPAWH AARGGWASALLVGKAPHPTGLVMRASSSPALPR AVLLTARAHCRLATASSLLMEWT*LRPGMTTPS PC*PLPPPPSPCCWSGRLGALFLPALCHIPHPPP LLLPPPA*PLPPPGCLGCRAWPPACWLPRWKGH TQWRRSVCQELGALWGLVLSEAPTIPATRLVSR SLVCSSPRCSRGAWPLAAACGLGTASWQ*TGKTC GMPRTKKQSVPCSGPAWSCRCWCGGTRHPRAYG NCASRRHLGRGWASASAGVPGATLATPATPQTR ASSSPR*APRGQPGATVGCVWVCGCWR*TSRACW A*RTARRCSCSAVWATPSPCWSVTALRPAPTQP WRCPQVSLPTPLRQASATGTAWRASLPSTGS*A LRAQARRRSCLDRPCTGGPRPQKPQVGVCSP*SW TTAPWPPCPALAACRGYRLEQLEGRWLNLPAPL VASSRPPRLLRMSCPPM*SRPTGPSRPCPLLTR LRMPLPSPPRLGLQPPRSSCPSGSGRSTLSWRCA CPRPRAPLSACPWWVLTTCGRCRRRKPENYSRR ERRCCGRRQRLGPKRGSPWTGRRWARRNRRMSS HPGPARAPPQGRARRPPHPWEVAPRCGRPKLNGA TRSGCACRVRSHRHPSVPCPLPSSGPWRPRSVR CGGQPG*SHWNRTLSEHRWSSAGPRKAGARGGP WSDWPRPLPLRPPRRPPLWKTSAPRPAPPRDACP CLERSLTTGPLRPCLLPDLSMTSSHRTLLRS*G PWNHLPALARRRRMEKWLWCFWAGPHPALWALK MWHCAAAAAP*GLGAVAWALCPPRGAGTSPRLGV GALPAPAPPLPQVF*PGC*HFKETPQEFWPVTN *LPHPSRDLGETVTSDVCTTKDSILWFKENKVD YIL

Frame 1

HAQVHPAVALQPARGVGGQAALFAAGRAGGDLP LQPQPGGAAARRQPAARAAQAFFPAAELAQAGP ERQRDPAVASRGGQLHAAGGAGRVPERYP*DPGE HQVLQGSGDRGLQREPPLQAP*WLHSAAQPGSP GPE*CVSAGTARGRGQPRQPGDPGAPGEPAQVP ASVPVISGQAGTAGSGRQRSGSAARHSGGSAQSS GAVA*PEPAVSTAPGAREPAAPGVPGRVGKPAG GAAC*ARRAGAAH*PAAVPEPAAEAARRHRSAE AAIHPKGRPESAVRGDRGHRGL*EPL*ADPHGEP ADGPAPLPGKAD*ADQPQRGPEPPRGAAARDRG LCGTQRPLLEGQPPGRPATRAGPHDRAARAGRG GEPPAESAVRAHPPQSQGPVAGREPGAAHAPVPD GG*CPDRREGAHLLLAAPAAPTQPRGCWAAGEP LGDLERCPAEPRQRHPVPGGPHR**GR*GSCS* EAGPTAPGHTSPQRAQGDEEEHRGAAERGLPLPA RLWVALACRGGEAAECRVWPE*RLSPICQHSL* G*ARGPVG*GTGWEPAGSHDCWRGGRRRRGLPG AHGAFRRGRTAARG*QGDRGGAA*GPLDPARREA AAHPQGHTSLQKALQDLQAAPARGRCGSAAGHA A*WGGPCGSRGLAQWPPRTLGSSGPEGGGGGGR G*SSGGGGRGGGGKQG*RGRGQH*GGGQGGGRGF CALCQGSVV*PGQ*PADRACSH*GGRADPHYPA ADWGPGHQHCGRQGLHTL*GGRRGHIHLSGVRG RPCGPGWSPCG*QAPGGEWCGSAGRRAPRGRGGA PGGRHCRADASVAGAHGGA*ERGHHHAAAARG* LQPPRAAGRGAAPAPAPA*EPRAPPSAPRGLPG TQREGAGLQHCWWERLHTLQGW*CGHLRLPHCRG RCCSPRGHTAGWRPRPLY*WSGRD*GQA*PRRL PADRCLPHHRPAVGAGGWGPSSSQPSATFLTPH RCCCHHQHNHCHPRGAWVAEPGPQPAGCRVGRAI PSGGDPSAKSWGPSGA*YCRRLRPFQPPVWCPG AWCVHLQGAPAGPGRSQRPAGWGPHPGSERARR AGCHAPRSSQCPAPALPGAVAAGAEGPGTPGPTG TVHPEGTWGEAGHQHPRGCQGPRWQPPRPHRRG HLHLQGEPHGGSRARRSAACGFAAVGGEPAEPA GPDARRGGAAAPQCGRHPHRAGL*RL*GQHRRSP GGVPRCHCQPLCGRHRPPEQPGEHLFHRPGAEP *GPRQGEGAAWTDPALGARGHRSRRSGSAAPEA GLPRPGRRAQRWQRAEGTVWSSWREDG*ISLLP* WPAAALPAFSG*AARQCEAGLQGLRGRAHFSPA *GCPCPAPHAWACSLPGAAVLPGAAEVL*AGGA RAPGRGPP*ARVPGGC*RPAEDAGGGSQKTTAEE SADAAGGGRGWGRSEARPGRGDAGRGGTGG*AA TLGQPEPHLKAEPGVPPTPGRWRPGADGQS*TA PPGAAARAESGATGTRACPVPCRAPGPGGREACA VEGSQDEVIGTGRSPSTDGPQQVPGRPGHAGAP GATGRGPFPCAHPVAHPCGRPRPPDQHLPGTPA LVWKEV*LQGLCGPAFFQTCL*HPVTGLC*GVEV PGTISQPWPAGGGWRSGSGASGQALTRRCGP*R CGTVQQPPPRKAWAPWPGPCALLEEQAPPPDLG WGPCQLQHHPCPKSFNLGVSILKRPHRSSGL*LT NCPTPAETSARL*LVMFVQPKTLFCGLRRIKLT TF

Frame 2

MLKCIPLWRCNRHVESVDKRHCSLQAVPEEIYR YSRSLEELLLDANQLRELPKPFFRLLNLRKLGL SDNEIQRLPPEVANFMQLVELDVSRNDIPEIPE SIKFCKALEIADFSGNPLSRLPDGFTQLRSLAHL ALNDVSLQALPGDVGNLANLVTLELRENLLKSL PASLSFLVKLEQLDLGGNDLEVLPDTLGALPNL RELWLDRNQLSALPPELGNLRRLVCLDVSENRLE ELPAELGGLVLLTDLLLSQNLLRRLPDGIGQLK QLSILKVDQNRLCEVTEAIGDCENLSELILTEN LLMALPRSLGKLTKLTNLNVDRNHLEALPPEIGG CVALSVLSLRDNRLAVLPPELAHTTELHVLDVA GNRLQSLPFALTHLNLKALWLAENQAQPMLRFQ TEDDARTGEKVLTCYLLPQQPPPSLEDAGQQGSL SETWSDAPPSRVSVIQFLEAPIGDEDAEEAAAE KRGLQRRATPHPSELKVMKRSIEGRRSEACPCQ PDSGSPLPAEEEKRLSAESGLSEDSRPSASTVSE AEPEGPSAEAQGGSQQEATTAGGEEDAEEDYQE PTVHFAEDALLPGDDREIEEGQPEAPWTLPGGR QRLIRKDTPHYKKHFKISKLPQPEAVVALLQGMQ PDGEGPVAPGGWHNGPHAPWAPRAQKEEEEEEE GSPQEEEVEEEEENRAEEEEASTEEEDKEGAVV SAPSVKGVSFDQANNLLIEPARIEEEELTLTILR QTGGLGISIAGGKGSTPYKGDDEGIFISRVSEE GPAARAGVRVGDKLLEVNGVALQGAEHHEAVEA LRGAGTAVQMRVWRERMVEPENAVTITPLRPEDD YSPRERRGGGLRLPLLPPESPGPLRQRHVACLA RSERGLGFSIAGGKGSTPYRAGDAGIFVSRIAE GGAAHRAGTLQVGDRVLSINGVDVTEARHDHAVS LLTAASPTIALLLEREAGGPLPPSPLPHSSPPT AAVATTSITTATPGVPGLPSLAPSLLAAALEGP YPVEEIRLPRAGGPLGLSIVGGSDHSSHPFGVQE PGVFISKVLPRGLAARSGLRVGDRILAVNGQDV RDATHQEAVSALLRPCLELSLLVRRDPAPPGLR ELCIQKAPGERLGISIRGGARGHAGNPRDPTDEG IFISKVSPTGAAGRDGRLRVGLRLLEVNQQSLL GLTHGEAVQLLRSVGDTLTVLVCDGFEASTDAA LEVSPGVIANPFAAGIGHRNSLESISSIDRELSP EGPGKEKELPGQTLHWGPEATEAAGRGLQPLKL DYRALAAVPSAGSVQRVPSGAAGGKMAESPCSP SGQQPPSPPSPDELPANVKQAYRAFAAVPTSHPP EDAPAQPPTPGPAASPEQLSFRERQKYFELEVR VPQAEGPPKRVSLVGADDLRKMQEEEARKLQQK RAQMLREAAEAGAEARLALDGETLGEEEQEDEQP PWASPSPTSRQSPASPPPLGGGAPVRTAKAERR HQERLRVQSPEPPAPERALSPAELRALEAEKRA LWRAARMKSLEQDALRAQMVLSRSQEGRGTRGPL ERLAEAPSPAPTPSPTPVEDLGPQTSTSPGRLP LSGKKFDYRAFAALPSSRPVYDIQSPDFAEELR SLEPSPSPGPQEEDGEVALVLLGRPSPGAVGPED VALCSSRRPVRPGRRGLGPVPS*RSRHLPQTWG GGPASSSTTLAPSLLTWVLAF*RDPTGVLACD* LTAPPQPRPRRDCN**CLYNQRLYFVV*GE*S*L HF