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Sequences

Sequence NM_182664

CGGGAGTAGCGCAGTCGCCAAAGCCGCCGCTGCCAAAGCTGCCGCCACTAGCCGGGCATGGCCATGGCGTCCCCGGCCATCGGGCAGCGCCCGTACCCGC TACTATTGGACCCCGAGCCGCCGCGCTATCTACAGAGCCTGAGCGGCCCCGAGCTACCGCCGCCGCCCCCCGACCGGTCCTCGCGCCTCTGTGTCCCGGC GCCCCTCTCCACTGCGCCCGGGGCGCGCGAGGGGCGCAGCGCCCGGAGGGCTGCCCGGGGGAACCTGGAGCCCCCGCCCCGGGCCTCCCGACCCGCTCGC CCGCTCCGGCCTGGTCTGCAGCAGAGACTGCGGCGGCGGCCTGGAGCGCCCCGACCCCGCGACGTGCGGAGCATCTTCGAGCAGCCGCAGGATCCCAGAG TCCCGGCGGAGCGAGGCGAGGGGCACTGCTTCGCCGAGTTGGTGCTGCCGGGCGGCCCCGGCTGGTGTGACCTGTGCGGACGAGAGGTGCTGCGGCAGGC GCTGCGCTGCACTAACTGTAAATTCACCTGTCACCCAGAATGCCGCAGCCTGATCCAGTTGGACTGCAGTCAGCAGGAGGGTTTATCCCGGGACAGACCC TCTCCAGAAAGCACCCTCACCGTGACCTTCAGCCAGAATGTCTGTAAACCTGTGGAGGAGACACAGCGCCCGCCCACACTGCAGGAGATCAAGCAGAAGA TCGACAGCTACAACACGCGAGAGAAGAACTGCCTGGGCATGAAACTGAGTGAAGACGGCACCTACACGGGTTTCATCAAAGTGCATCTGAAACTCCGGCG GCCTGTGACGGTGCCTGCTGGGATCCGGCCCCAGTCCATCTATGATGCCATCAAGGAGGTGAACCTGGCGGCTACCACGGACAAGCGGACATCCTTCTAC CTGCCCCTAGATGCCATCAAGCAGCTGCACATCAGCAGCACCACCACCGTCAGTGAGGTCATCCAGGGGCTGCTCAAGAAGTTCATGGTTGTGGACAATC CCCAGAAGTTTGCACTTTTTAAGCGGATACACAAGGACGGACAAGTGGGATGCCTTCTCCATCCCTGAACTTCAGAACTTCCTAACAATCCTGGAAAAAG AGGAGCAGGACAAAATCCAACAAGTGCAAAAGAAGTATGACAAGTTTAGGCAGAAACTGGAGGAGGCCTTAAGAGAATCCCAGGGCAAACCTGGGTAACC GGTCCTGCTTCCTCTCCTCCTGGTGCATTCAGATTTATTTGTATTATTAATTATTATTTTGCAACAGACACTTTTTCTCAGGACATCTCTGGCAGGTGCA TTTGTGCCTGCCCAGCAGTTCCAGCTGTGGCAAAAGTCTCTTCCATGGACAAGTGTTTGCATGGGGGTTCAGCTGTGCCCGCCCCCAGGCTGTGCCCCAC CACAGATTCTGCCAAGGATCAGAACTCATGTGAAACAAACAGCTGACGTCCTCTCTCGATCTGCAAGCCTTTCACCAACCAAATAGTTGCCTCTCTCGTC ACCAAACTGGAACCTCACACCAGCCGGCAAAGGAAGGAAGAAAGGTTTTAGAGCTGTGTGTTCTTTCTCTGGCTTTGATTCTTCTTTGAGTTCTCTTACT TGCCACGTACAGGACCATTATTTATGAGTGAAAAGTTGTAGCACATTCCTTTTGCAGGTCTGAGCTAAGCCCCTGAAAGCAGGGTAATGCTCATAAAAGG ACTGTTCCCGCGGCCCCAAGGTGCCTGTTGTTCACACTTAAGGGAAGTTTATAAAGCTACTGGCCCCAGATGCTCAGGGTAAGGAGCACCAAAGCTGAGG CTGGCTCAGAGATCTCCAGAGAAGCTGCAGCCTGCCCTGGCCCTGGCTCTGGCCCTGGCCCACATTGCACATGGAAACCCAAAGGCATATATCTGCGTAT GTGTGGTACTTAGTCACATCTTTGTCAACAAACTGTTCGTTTTTAAGTTACAAATTTGAATTTAATGTTGTCATCATCGTCATGTGTTTCCCCAAAGGGA AGCCAGTCATTGACCATTTAAAAAGTCTCCTGCTAAGTATGGAAATCAGACAGTAAGAGAAAGCCAAAAAGCAATGCAGAGAAAGGTGTCCAAGCTGTCT TCAGCCTTCCCCAGCTAAAGAGCAGAGGAGGGCCTGGGCTACTTGGGTTCCCCATCGGCCTCCAGCACTGCCTCCCTCCTCCCACTGCGACTCTGGGATC TCCAGGTGCTGCCCAAGGAGTTGCCTTGATTACAGAGAGGGGAGCCTCCAATTCGGCCAACTTGGAGTCCTTTCTGTTTTGAAGCATGGGCCAGACCCGG CACTGCGCTCGGAGAGCCGGTGGGCCTGGCCTCCCCGTCGACCTCAGTGCCTTTTTGTTTTCAGAGAGAAATAGGAGTAGGGCGAGTTTGCCTGAAGCTC TGCTGCTGGCTTCTCCTGCCAGGAAGTGAACAATGGCGGCGGTGTGGGAGACAAGGCCAGGAGAGCCCGCGTTCAGTATGGGTTGAGGGTCACAGACCTC CCTCCCATCTGGGTGCCTGAGTTTTGACTCCAATCAGTGATACCAGACCACATTGACAGGGAGGATCAAATTCCTGACTTACATTTGCACTGGCTTCTTG TTTAGGCTGAATCCTAAAATAAATTAGTCAAAAAATTCCAACAAGTAGCCAGGACTGCAGAGACACTCCAGTGCAGAGGGAGAAGGACTTGTAATTTTCA AAGCAGGGCTGGTTTTCCAACCCAGCCTCTGAGAAACCATTTCTTTGCTATCCTCTGCCTTCCCAAGTCCCTCTTGGGTCGGTTCAAGCCCAAGCTTGTT CGTGTAGCTTCAGAAGTTCCCTCTCTGACCCAGGCTGAGTCCATACTGCCCCTGATCCCAGAAGGAATGCTGACCCCTCGTCGTATGAACTGTGCATAGT CTCCAGAGCTTCAAAGGCAACACAAGCTCGCAACTCTAAGATTTTTTTAAACCACAAAAACCCTGGTTAGCCATCTCATGCTCAGCCTTATCACTTCCCT CCCTTTAGAAACTCTCTCCCTGCTGTATATTAAAGGGAGCAGGTGGAGAGTCATTTTCCTTCGTCCTGCATGTCTCTAACATTAATAGAAGGCATGGCTC CTGCTGCAACCGCTGTGAATGCTGCTGAGAACCTCCCTCTATGGGGATGGCTATTTTATTTTTGAGAAGGAAAAAAAAAGTCATGTATATATACACATAA AGGCATATAGCTATATATAAAGAGATAAGGGTGTTTATGAAATGAGAAAATTATTGGACAATTCAGACTTTACTAAAGCACAGTTAGACCCAAGGCCTAT GCTGAGGTCTAAACCTCTGAAAAAAGTATAGTATCGAGTACCCGTTCCCTCCCAGAGGTGGGAGTAACTGCTGGTAGTGCCTTCTTTGGTTGTGTTGCTC AGTGTGTAAGTGTTTGTTTCCAGGATATTTTCTTTTTAAATGTCTTTCTTATATGGGTTTTAAAAAAAAGTAATAAAAGCCTGTTGCAAAAA

refseq_RASSF5.F1 (forward primer)
refseq_RASSF5.R1 (reverse primer)
Single exon skipping

Frame 0

RE*RSRQSRRCQSCRH*PGMAMASPAIGQRPYP LLLDPEPPRYLQSLSGPELPPPPPDRSSRLCVPA PLSTAPGAREGRSARRAARGNLEPPPRASRPAR PLRPGLQQRLRRRPGAPRPRDVRSIFEQPQDPR VPAERGEGHCFAELVLPGGPGWCDLCGREVLRQA LRCTNCKFTCHPECRSLIQLDCSQQEGLSRDRP SPESTLTVTFSQNVCKPVEETQRPPTLQEIKQK IDSYNTREKNCLGMKLSEDGTYTGFIKVHLKLRR PVTVPAGIRPQSIYDAIKEVNLAATTDKRTSFY LPLDAIKQLHISSTTTVSEVIQGLLKKFMVVDN PQKFALFKRIHKDGQVGCLLHP*TSELPNNPGKR GAGQNPTSAKEV*QV*AETGGGLKRIPGQTWVT GPASSPPGAFRFICIINYYFATDTFSQDISGRC ICACPAVPAVAKVSSMDKCLHGGSAVPAPRLCPT TDSAKDQNSCETNS*RPLSICKPFTNQIVASLV TKLEPHTSRQRKEERF*SCVFFLWL*FFFEFSY LPRTGPLFMSEKL*HIPFAGLS*APESRVMLIKG LFPRPQGACCSHLREVYKATGPRCSG*GAPKLR LAQRSPEKLQPALALALALAHIAHGNPKAYICV CVVLSHIFVNKLFVFKLQI*I*CCHHRHVFPQRE ASH*PFKKSPAKYGNQTVRESQKAMQRKVSKLS SAFPS*RAEEGLGYLGSPSASSTASLLPLRLWD LQVLPKELP*LQRGEPPIRPTWSPFCFEAWARPG TALGEPVGLASPSTSVPFCFQREIGVGRVCLKL CCWLLLPGSEQWRRCGRQGQESPRSVWVEGHRP PSHLGA*VLTPISDTRPH*QGGSNS*LTFALASC LG*ILK*ISQKIPTSSQDCRDTPVQREKDL*FS KQGWFSNPASEKPFLCYPLPSQVPLGSVQAQAC SCSFRSSLSDPG*VHTAPDPRRNADPSSYELCIV SRASKATQARNSKIFLNHKNPG*PSHAQPYHFP PFRNSLPAVY*REQVESHFPSSCMSLTLIEGMA PAATAVNAAENLPLWGWLFYF*EGKKKSCIYTHK GI*LYIKR*GCL*NEKIIGQFRLY*STVRPKAY AEV*TSEKSIVSSTRSLPEVGVTAGSAFFGCVA QCVSVCFQDIFFLNVFLIWVLKKSNKSLLQK

Frame 1

GSSAVAKAAAAKAAATSRAWPWRPRPSGSARTR YYWTPSRRAIYRA*AAPSYRRRPPTGPRASVSR RPSPLRPGRARGAAPGGLPGGTWSPRPGPPDPLA RSGLVCSRDCGGGLERPDPATCGASSSSRRIPE SRRSEARGTASPSWCCRAAPAGVTCADERCCGR RCAALTVNSPVTQNAAA*SSWTAVSRRVYPGTDP LQKAPSP*PSARMSVNLWRRHSARPHCRRSSRR STATTRERRTAWA*N*VKTAPTRVSSKCI*NSG GL*RCLLGSGPSPSMMPSRR*TWRLPRTSGHPST CP*MPSSSCTSAAPPPSVRSSRGCSRSSWLWTI PRSLHFLSGYTRTDKWDAFSIPELQNFLTILEK EEQDKIQQVQKKYDKFRQKLEEALRESQGKPG*P VLLPLLLVHSDLFVLLIIILQQTLFLRTSLAGA FVPAQQFQLWQKSLPWTSVCMGVQLCPPPGCAP PQILPRIRTHVKQTADVLSRSASLSPTK*LPLSS PNWNLTPAGKGRKKGFRAVCSFSGFDSSLSSLT CHVQDHYL*VKSCSTFLLQV*AKPLKAG*CS*K DCSRGPKVPVVHT*GKFIKLLAPDAQGKEHQS*G WLRDLQRSCSLPWPWLWPWPTLHMETQRHISAY VWYLVTSLSTNCSFLSYKFEFNVVIIVMCFPKG KPVIDHLKSLLLSMEIRQ*EKAKKQCRERCPSCL QPSPAKEQRRAWATWVPHRPPALPPSSHCDSGI SRCCPRSCLDYREGSLQFGQLGVLSVLKHGPDP ALRSESRWAWPPRRPQCLFVFREK*E*GEFA*SS AAGFSCQEVNNGGGVGDKARRARVQYGLRVTDL PPIWVPEF*LQSVIPDHIDREDQIPDLHLHWLL V*AES*NKLVKKFQQVARTAETLQCRGRRTCNFQ SRAGFPTQPLRNHFFAILCLPKSLLGRFKPKLV RVASEVPSLTQAESILPLIPEGMLTPRRMNCA* SPELQRQHKLATLRFF*TTKTLVSHLMLSLITSL PLETLSLLYIKGSRWRVIFLRPACL*H**KAWL LLQPL*MLLRTSLYGDGYFIFEKEKKSHVYIHI KAYSYI*RDKGVYEMRKLLDNSDFTKAQLDPRPM LRSKPLKKV*YRVPVPSQRWE*LLVVPSLVVLL SV*VFVSRIFSF*MSFLYGF*KKVIKACCK

Frame 2

GVAQSPKPPLPKLPPLAGHGHGVPGHRAAPVPA TIGPRAAALSTEPERPRATAAAPRPVLAPLCPG APLHCARGARGAQRPEGCPGEPGAPAPGLPTRS PAPAWSAAETAAAAWSAPTPRRAEHLRAAAGSQS PGGARRGALLRRVGAAGRPRLV*PVRTRGAAAG AALH*L*IHLSPRMPQPDPVGLQSAGGFIPGQT LSRKHPHRDLQPECL*TCGGDTAPAHTAGDQAED RQLQHAREELPGHETE*RRHLHGFHQSASETPA ACDGACWDPAPVHL*CHQGGEPGGYHGQADILL PAPRCHQAAAHQQHHHRQ*GHPGAAQEVHGCGQS PEVCTF*ADTQGRTSGMPSPSLNFRTS*QSWKK RSRTKSNKCKRSMTSLGRNWRRP*ENPRANLGN RSCFLSSWCIQIYLYY*LLFCNRHFFSGHLWQVH LCLPSSSSCGKSLFHGQVFAWGFSCARPQAVPH HRFCQGSELM*NKQLTSSLDLQAFHQPNSCLSR HQTGTSHQPAKEGRKVLELCVLSLALILL*VLLL ATYRTIIYE*KVVAHSFCRSELSP*KQGNAHKR TVPAAPRCLLFTLKGSL*SYWPQMLRVRSTKAE AGSEISREAAACPGPGSGPGPHCTWKPKGIYLRM CGT*SHLCQQTVRF*VTNLNLMLSSSSCVSPKG SQSLTI*KVSC*VWKSDSKRKPKSNAEKGVQAV FSLPQLKSRGGPGLLGFPIGLQHCLPPPTATLGS PGAAQGVALITERGASNSANLESFLF*SMGQTR HCARRAGGPGLPVDLSAFLFSERNRSRASLPEA LLLASPARK*TMAAVWETRPGEPAFSMG*GSQTS LPSGCLSFDSNQ*YQTTLTGRIKFLTYICTGFL FRLNPKIN*SKNSNK*PGLQRHSSAEGEGLVIF KAGLVFQPSL*ETISLLSSAFPSPSWVGSSPSLF V*LQKFPL*PRLSPYCP*SQKEC*PLVV*TVHS LQSFKGNTSSQL*DFFKPQKPWLAISCSALSLP SL*KLSPCCILKGAGGESFSFVLHVSNINRRHGS CCNRCECC*EPPSMGMAILFLRRKKKVMYIYT* RHIAIYKEIRVFMK*ENYWTIQTLLKHS*TQGL C*GLNL*KKYSIEYPFPPRGGSNCW*CLLWLCCS VCKCLFPGYFLFKCLSYMGFKKK**KPVAK

Sequence NM_182665

GCAGGAAAGGCGCGGGAGGCGGGGGAGGTGCGGAGATGGCGCTCTGCACGGCGGCGGAGGGAGGGCGCTGGCGCCGGGGACACGAAACCGCAGAGCCCGG ACGAGTCAGGGAGTGAGGCGCGAGCCGGGCGCCCGGGGCTCTGCAGGCGCAGGCGGCGCGGGGACAGGAGCAGGTTACCGGGCCGCCCGAGCGCTCGCAC CCCGCTGAAAGAAACGCAGGCGGCCCGCCGGCTCTGCCTGGTCCGCTACCCGACCAGCTCCCGGCTCGGGGCTCAGAGCTAGGGGCTTACGCCAAGCGGA GCCCGGGGAGGGGTGCCCACCTCCCTCCGCCGCATCCCAAGCCCGGCCCCCTTGATGCGCTGGCGGCCTCGGCCGGGAACTCCGGGGTAGATGACCGTGG ACAGCAGCATGAGCAGTGGGTACTGCAGCCTGGACGAGGAACTGGAAGACTGCTTCTTCACTGCTAAGACTACCTTTTTCAGAAATGCGCAGAGCAAACA TCTTTCAAAGAATGTCTGTAAACCTGTGGAGGAGACACAGCGCCCGCCCACACTGCAGGAGATCAAGCAGAAGATCGACAGCTACAACACGCGAGAGAAG AACTGCCTGGGCATGAAACTGAGTGAAGACGGCACCTACACGGGTTTCATCAAAGTGCATCTGAAACTCCGGCGGCCTGTGACGGTGCCTGCTGGGATCC GGCCCCAGTCCATCTATGATGCCATCAAGGAGGTGAACCTGGCGGCTACCACGGACAAGCGGACATCCTTCTACCTGCCCCTAGATGCCATCAAGCAGCT GCACATCAGCAGCACCACCACCGTCAGTGAGGTCATCCAGGGGCTGCTCAAGAAGTTCATGGTTGTGGACAATCCCCAGAAGTTTGCACTTTTTAAGCGG ATACACAAGGACGGACAAGTGCTCTTCCAGAAACTCTCCATTGCTGACCGCCCCCTCTACCTGCGCCTGCTTGCTGGGCCTGACACGGAGGTCCTCAGCT TTGTGCTAAAGGAGAATGAAACTGGAGAGGTAGAGTGGGATGCCTTCTCCATCCCTGAACTTCAGAACTTCCTAACAATCCTGGAAAAAGAGGAGCAGGA CAAAATCCAACAAGTGCAAAAGAAGTATGACAAGTTTAGGCAGAAACTGGAGGAGGCCTTAAGAGAATCCCAGGGCAAACCTGGGTAACCGGTCCTGCTT CCTCTCCTCCTGGTGCATTCAGATTTATTTGTATTATTAATTATTATTTTGCAACAGACACTTTTTCTCAGGACATCTCTGGCAGGTGCATTTGTGCCTG CCCAGCAGTTCCAGCTGTGGCAAAAGTCTCTTCCATGGACAAGTGTTTGCATGGGGGTTCAGCTGTGCCCGCCCCCAGGCTGTGCCCCACCACAGATTCT GCCAAGGATCAGAACTCATGTGAAACAAACAGCTGACGTCCTCTCTCGATCTGCAAGCCTTTCACCAACCAAATAGTTGCCTCTCTCGTCACCAAACTGG AACCTCACACCAGCCGGCAAAGGAAGGAAGAAAGGTTTTAGAGCTGTGTGTTCTTTCTCTGGCTTTGATTCTTCTTTGAGTTCTCTTACTTGCCACGTAC AGGACCATTATTTATGAGTGAAAAGTTGTAGCACATTCCTTTTGCAGGTCTGAGCTAAGCCCCTGAAAGCAGGGTAATGCTCATAAAAGGACTGTTCCCG CGGCCCCAAGGTGCCTGTTGTTCACACTTAAGGGAAGTTTATAAAGCTACTGGCCCCAGATGCTCAGGGTAAGGAGCACCAAAGCTGAGGCTGGCTCAGA GATCTCCAGAGAAGCTGCAGCCTGCCCTGGCCCTGGCTCTGGCCCTGGCCCACATTGCACATGGAAACCCAAAGGCATATATCTGCGTATGTGTGGTACT TAGTCACATCTTTGTCAACAAACTGTTCGTTTTTAAGTTACAAATTTGAATTTAATGTTGTCATCATCGTCATGTGTTTCCCCAAAGGGAAGCCAGTCAT TGACCATTTAAAAAGTCTCCTGCTAAGTATGGAAATCAGACAGTAAGAGAAAGCCAAAAAGCAATGCAGAGAAAGGTGTCCAAGCTGTCTTCAGCCTTCC CCAGCTAAAGAGCAGAGGAGGGCCTGGGCTACTTGGGTTCCCCATCGGCCTCCAGCACTGCCTCCCTCCTCCCACTGCGACTCTGGGATCTCCAGGTGCT GCCCAAGGAGTTGCCTTGATTACAGAGAGGGGAGCCTCCAATTCGGCCAACTTGGAGTCCTTTCTGTTTTGAAGCATGGGCCAGACCCGGCACTGCGCTC GGAGAGCCGGTGGGCCTGGCCTCCCCGTCGACCTCAGTGCCTTTTTGTTTTCAGAGAGAAATAGGAGTAGGGCGAGTTTGCCTGAAGCTCTGCTGCTGGC TTCTCCTGCCAGGAAGTGAACAATGGCGGCGGTGTGGGAGACAAGGCCAGGAGAGCCCGCGTTCAGTATGGGTTGAGGGTCACAGACCTCCCTCCCATCT GGGTGCCTGAGTTTTGACTCCAATCAGTGATACCAGACCACATTGACAGGGAGGATCAAATTCCTGACTTACATTTGCACTGGCTTCTTGTTTAGGCTGA ATCCTAAAATAAATTAGTCAAAAAATTCCAACAAGTAGCCAGGACTGCAGAGACACTCCAGTGCAGAGGGAGAAGGACTTGTAATTTTCAAAGCAGGGCT GGTTTTCCAACCCAGCCTCTGAGAAACCATTTCTTTGCTATCCTCTGCCTTCCCAAGTCCCTCTTGGGTCGGTTCAAGCCCAAGCTTGTTCGTGTAGCTT CAGAAGTTCCCTCTCTGACCCAGGCTGAGTCCATACTGCCCCTGATCCCAGAAGGAATGCTGACCCCTCGTCGTATGAACTGTGCATAGTCTCCAGAGCT TCAAAGGCAACACAAGCTCGCAACTCTAAGATTTTTTTAAACCACAAAAACCCTGGTTAGCCATCTCATGCTCAGCCTTATCACTTCCCTCCCTTTAGAA ACTCTCTCCCTGCTGTATATTAAAGGGAGCAGGTGGAGAGTCATTTTCCTTCGTCCTGCATGTCTCTAACATTAATAGAAGGCATGGCTCCTGCTGCAAC CGCTGTGAATGCTGCTGAGAACCTCCCTCTATGGGGATGGCTATTTTATTTTTGAGAAGGAAAAAAAAAGTCATGTATATATACACATAAAGGCATATAG CTATATATAAAGAGATAAGGGTGTTTATGAAATGAGAAAATTATTGGACAATTCAGACTTTACTAAAGCACAGTTAGACCCAAGGCCTATGCTGAGGTCT AAACCTCTGAAAAAAGTATAGTATCGAGTACCCGTTCCCTCCCAGAGGTGGGAGTAACTGCTGGTAGTGCCTTCTTTGGTTGTGTTGCTCAGTGTGTAAG TGTTTGTTTCCAGGATATTTTCTTTTTAAATGTCTTTCTTATATGGGTTTTAAAAAAAAGTAATAAAAGCCTGTTGCAAAAA

refseq_RASSF5.F1 (forward primer)
refseq_RASSF5.R1 (reverse primer)
Single exon skipping

Frame 0

AGKAREAGEVRRWRSARRRREGAGAGDTKPQSP DESGSEARAGRPGLCRRRRRGDRSRLPGRPSART PLKETQAARRLCLVRYPTSSRLGAQS*GLTPSG ARGGVPTSLRRIPSPAPLMRWRPRPGTPG*MTV DSSMSSGYCSLDEELEDCFFTAKTTFFRNAQSKH LSKNVCKPVEETQRPPTLQEIKQKIDSYNTREK NCLGMKLSEDGTYTGFIKVHLKLRRPVTVPAGI RPQSIYDAIKEVNLAATTDKRTSFYLPLDAIKQL HISSTTTVSEVIQGLLKKFMVVDNPQKFALFKR IHKDGQVLFQKLSIADRPLYLRLLAGPDTEVLS FVLKENETGEVEWDAFSIPELQNFLTILEKEEQD KIQQVQKKYDKFRQKLEEALRESQGKPG*PVLL PLLLVHSDLFVLLIIILQQTLFLRTSLAGAFVP AQQFQLWQKSLPWTSVCMGVQLCPPPGCAPPQIL PRIRTHVKQTADVLSRSASLSPTK*LPLSSPNW NLTPAGKGRKKGFRAVCSFSGFDSSLSSLTCHV QDHYL*VKSCSTFLLQV*AKPLKAG*CS*KDCSR GPKVPVVHT*GKFIKLLAPDAQGKEHQS*GWLR DLQRSCSLPWPWLWPWPTLHMETQRHISAYVWY LVTSLSTNCSFLSYKFEFNVVIIVMCFPKGKPVI DHLKSLLLSMEIRQ*EKAKKQCRERCPSCLQPS PAKEQRRAWATWVPHRPPALPPSSHCDSGISRC CPRSCLDYREGSLQFGQLGVLSVLKHGPDPALRS ESRWAWPPRRPQCLFVFREK*E*GEFA*SSAAG FSCQEVNNGGGVGDKARRARVQYGLRVTDLPPI WVPEF*LQSVIPDHIDREDQIPDLHLHWLLV*AE S*NKLVKKFQQVARTAETLQCRGRRTCNFQSRA GFPTQPLRNHFFAILCLPKSLLGRFKPKLVRVA SEVPSLTQAESILPLIPEGMLTPRRMNCA*SPEL QRQHKLATLRFF*TTKTLVSHLMLSLITSLPLE TLSLLYIKGSRWRVIFLRPACL*H**KAWLLLQ PL*MLLRTSLYGDGYFIFEKEKKSHVYIHIKAYS YI*RDKGVYEMRKLLDNSDFTKAQLDPRPMLRS KPLKKV*YRVPVPSQRWE*LLVVPSLVVLLSV* VFVSRIFSF*MSFLYGF*KKVIKACCK

Frame 1

QERRGRRGRCGDGALHGGGGRALAPGTRNRRAR TSQGVRREPGARGSAGAGGAGTGAGYRAARALA PR*KKRRRPAGSAWSATRPAPGSGLRARGLRQAE PGEGCPPPSAASQARPP*CAGGLGRELRGR*PW TAA*AVGTAAWTRNWKTASSLLRLPFSEMRRAN IFQRMSVNLWRRHSARPHCRRSSRRSTATTRERR TAWA*N*VKTAPTRVSSKCI*NSGGL*RCLLGS GPSPSMMPSRR*TWRLPRTSGHPSTCP*MPSSS CTSAAPPPSVRSSRGCSRSSWLWTIPRSLHFLSG YTRTDKCSSRNSPLLTAPSTCACLLGLTRRSSA LC*RRMKLER*SGMPSPSLNFRTS*QSWKKRSR TKSNKCKRSMTSLGRNWRRP*ENPRANLGNRSCF LSSWCIQIYLYY*LLFCNRHFFSGHLWQVHLCL PSSSSCGKSLFHGQVFAWGFSCARPQAVPHHRF CQGSELM*NKQLTSSLDLQAFHQPNSCLSRHQTG TSHQPAKEGRKVLELCVLSLALILL*VLLLATY RTIIYE*KVVAHSFCRSELSP*KQGNAHKRTVP AAPRCLLFTLKGSL*SYWPQMLRVRSTKAEAGSE ISREAAACPGPGSGPGPHCTWKPKGIYLRMCGT *SHLCQQTVRF*VTNLNLMLSSSSCVSPKGSQS LTI*KVSC*VWKSDSKRKPKSNAEKGVQAVFSLP QLKSRGGPGLLGFPIGLQHCLPPPTATLGSPGA AQGVALITERGASNSANLESFLF*SMGQTRHCA RRAGGPGLPVDLSAFLFSERNRSRASLPEALLLA SPARK*TMAAVWETRPGEPAFSMG*GSQTSLPS GCLSFDSNQ*YQTTLTGRIKFLTYICTGFLFRL NPKIN*SKNSNK*PGLQRHSSAEGEGLVIFKAGL VFQPSL*ETISLLSSAFPSPSWVGSSPSLFV*L QKFPL*PRLSPYCP*SQKEC*PLVV*TVHSLQS FKGNTSSQL*DFFKPQKPWLAISCSALSLPSL*K LSPCCILKGAGGESFSFVLHVSNINRRHGSCCN RCECC*EPPSMGMAILFLRRKKKVMYIYT*RHI AIYKEIRVFMK*ENYWTIQTLLKHS*TQGLC*GL NL*KKYSIEYPFPPRGGSNCW*CLLWLCCSVCK CLFPGYFLFKCLSYMGFKKK**KPVAK

Frame 2

RKGAGGGGGAEMALCTAAEGGRWRRGHETAEPG RVRE*GASRAPGALQAQAARGQEQVTGPPERSH PAERNAGGPPALPGPLPDQLPARGSELGAYAKR SPGRGAHLPPPHPKPGPLDALAASAGNSGVDDRG QQHEQWVLQPGRGTGRLLLHC*DYLFQKCAEQT SFKECL*TCGGDTAPAHTAGDQAEDRQLQHARE ELPGHETE*RRHLHGFHQSASETPAACDGACWDP APVHL*CHQGGEPGGYHGQADILLPAPRCHQAA AHQQHHHRQ*GHPGAAQEVHGCGQSPEVCTF*A DTQGRTSALPETLHC*PPPLPAPACWA*HGGPQL CAKGE*NWRGRVGCLLHP*TSELPNNPGKRGAG QNPTSAKEV*QV*AETGGGLKRIPGQTWVTGPA SSPPGAFRFICIINYYFATDTFSQDISGRCICAC PAVPAVAKVSSMDKCLHGGSAVPAPRLCPTTDS AKDQNSCETNS*RPLSICKPFTNQIVASLVTKL EPHTSRQRKEERF*SCVFFLWL*FFFEFSYLPRT GPLFMSEKL*HIPFAGLS*APESRVMLIKGLFP RPQGACCSHLREVYKATGPRCSG*GAPKLRLAQ RSPEKLQPALALALALAHIAHGNPKAYICVCVVL SHIFVNKLFVFKLQI*I*CCHHRHVFPQREASH *PFKKSPAKYGNQTVRESQKAMQRKVSKLSSAF PS*RAEEGLGYLGSPSASSTASLLPLRLWDLQVL PKELP*LQRGEPPIRPTWSPFCFEAWARPGTAL GEPVGLASPSTSVPFCFQREIGVGRVCLKLCCW LLLPGSEQWRRCGRQGQESPRSVWVEGHRPPSHL GA*VLTPISDTRPH*QGGSNS*LTFALASCLG* ILK*ISQKIPTSSQDCRDTPVQREKDL*FSKQG WFSNPASEKPFLCYPLPSQVPLGSVQAQACSCSF RSSLSDPG*VHTAPDPRRNADPSSYELCIVSRA SKATQARNSKIFLNHKNPG*PSHAQPYHFPPFR NSLPAVY*REQVESHFPSSCMSLTLIEGMAPAAT AVNAAENLPLWGWLFYF*EGKKKSCIYTHKGI* LYIKR*GCL*NEKIIGQFRLY*STVRPKAYAEV *TSEKSIVSSTRSLPEVGVTAGSAFFGCVAQCVS VCFQDIFFLNVFLIWVLKKSNKSLLQK

Sequence NM_182663

CGGGAGTAGCGCAGTCGCCAAAGCCGCCGCTGCCAAAGCTGCCGCCACTAGCCGGGCATGGCCATGGCGTCCCCGGCCATCGGGCAGCGCCCGTACCCGC TACTATTGGACCCCGAGCCGCCGCGCTATCTACAGAGCCTGAGCGGCCCCGAGCTACCGCCGCCGCCCCCCGACCGGTCCTCGCGCCTCTGTGTCCCGGC GCCCCTCTCCACTGCGCCCGGGGCGCGCGAGGGGCGCAGCGCCCGGAGGGCTGCCCGGGGGAACCTGGAGCCCCCGCCCCGGGCCTCCCGACCCGCTCGC CCGCTCCGGCCTGGTCTGCAGCAGAGACTGCGGCGGCGGCCTGGAGCGCCCCGACCCCGCGACGTGCGGAGCATCTTCGAGCAGCCGCAGGATCCCAGAG TCCCGGCGGAGCGAGGCGAGGGGCACTGCTTCGCCGAGTTGGTGCTGCCGGGCGGCCCCGGCTGGTGTGACCTGTGCGGACGAGAGGTGCTGCGGCAGGC GCTGCGCTGCACTAACTGTAAATTCACCTGTCACCCAGAATGCCGCAGCCTGATCCAGTTGGACTGCAGTCAGCAGGAGGGTTTATCCCGGGACAGACCC TCTCCAGAAAGCACCCTCACCGTGACCTTCAGCCAGAATGTCTGTAAACCTGTGGAGGAGACACAGCGCCCGCCCACACTGCAGGAGATCAAGCAGAAGA TCGACAGCTACAACACGCGAGAGAAGAACTGCCTGGGCATGAAACTGAGTGAAGACGGCACCTACACGGGTTTCATCAAAGTGCATCTGAAACTCCGGCG GCCTGTGACGGTGCCTGCTGGGATCCGGCCCCAGTCCATCTATGATGCCATCAAGGAGGTGAACCTGGCGGCTACCACGGACAAGCGGACATCCTTCTAC CTGCCCCTAGATGCCATCAAGCAGCTGCACATCAGCAGCACCACCACCGTCAGTGAGGTCATCCAGGGGCTGCTCAAGAAGTTCATGGTTGTGGACAATC CCCAGAAGTTTGCACTTTTTAAGCGGATACACAAGGACGGACAAGTGCTCTTCCAGAAACTCTCCATTGCTGACCGCCCCCTCTACCTGCGCCTGCTTGC TGGGCCTGACACGGAGGTCCTCAGCTTTGTGCTAAAGGAGAATGAAACTGGAGAGGTAGAGTGGGATGCCTTCTCCATCCCTGAACTTCAGAACTTCCTA ACAATCCTGGAAAAAGAGGAGCAGGACAAAATCCAACAAGTGCAAAAGAAGTATGACAAGTTTAGGCAGAAACTGGAGGAGGCCTTAAGAGAATCCCAGG GCAAACCTGGGTAACCGGTCCTGCTTCCTCTCCTCCTGGTGCATTCAGATTTATTTGTATTATTAATTATTATTTTGCAACAGACACTTTTTCTCAGGAC ATCTCTGGCAGGTGCATTTGTGCCTGCCCAGCAGTTCCAGCTGTGGCAAAAGTCTCTTCCATGGACAAGTGTTTGCATGGGGGTTCAGCTGTGCCCGCCC CCAGGCTGTGCCCCACCACAGATTCTGCCAAGGATCAGAACTCATGTGAAACAAACAGCTGACGTCCTCTCTCGATCTGCAAGCCTTTCACCAACCAAAT AGTTGCCTCTCTCGTCACCAAACTGGAACCTCACACCAGCCGGCAAAGGAAGGAAGAAAGGTTTTAGAGCTGTGTGTTCTTTCTCTGGCTTTGATTCTTC TTTGAGTTCTCTTACTTGCCACGTACAGGACCATTATTTATGAGTGAAAAGTTGTAGCACATTCCTTTTGCAGGTCTGAGCTAAGCCCCTGAAAGCAGGG TAATGCTCATAAAAGGACTGTTCCCGCGGCCCCAAGGTGCCTGTTGTTCACACTTAAGGGAAGTTTATAAAGCTACTGGCCCCAGATGCTCAGGGTAAGG AGCACCAAAGCTGAGGCTGGCTCAGAGATCTCCAGAGAAGCTGCAGCCTGCCCTGGCCCTGGCTCTGGCCCTGGCCCACATTGCACATGGAAACCCAAAG GCATATATCTGCGTATGTGTGGTACTTAGTCACATCTTTGTCAACAAACTGTTCGTTTTTAAGTTACAAATTTGAATTTAATGTTGTCATCATCGTCATG TGTTTCCCCAAAGGGAAGCCAGTCATTGACCATTTAAAAAGTCTCCTGCTAAGTATGGAAATCAGACAGTAAGAGAAAGCCAAAAAGCAATGCAGAGAAA GGTGTCCAAGCTGTCTTCAGCCTTCCCCAGCTAAAGAGCAGAGGAGGGCCTGGGCTACTTGGGTTCCCCATCGGCCTCCAGCACTGCCTCCCTCCTCCCA CTGCGACTCTGGGATCTCCAGGTGCTGCCCAAGGAGTTGCCTTGATTACAGAGAGGGGAGCCTCCAATTCGGCCAACTTGGAGTCCTTTCTGTTTTGAAG CATGGGCCAGACCCGGCACTGCGCTCGGAGAGCCGGTGGGCCTGGCCTCCCCGTCGACCTCAGTGCCTTTTTGTTTTCAGAGAGAAATAGGAGTAGGGCG AGTTTGCCTGAAGCTCTGCTGCTGGCTTCTCCTGCCAGGAAGTGAACAATGGCGGCGGTGTGGGAGACAAGGCCAGGAGAGCCCGCGTTCAGTATGGGTT GAGGGTCACAGACCTCCCTCCCATCTGGGTGCCTGAGTTTTGACTCCAATCAGTGATACCAGACCACATTGACAGGGAGGATCAAATTCCTGACTTACAT TTGCACTGGCTTCTTGTTTAGGCTGAATCCTAAAATAAATTAGTCAAAAAATTCCAACAAGTAGCCAGGACTGCAGAGACACTCCAGTGCAGAGGGAGAA GGACTTGTAATTTTCAAAGCAGGGCTGGTTTTCCAACCCAGCCTCTGAGAAACCATTTCTTTGCTATCCTCTGCCTTCCCAAGTCCCTCTTGGGTCGGTT CAAGCCCAAGCTTGTTCGTGTAGCTTCAGAAGTTCCCTCTCTGACCCAGGCTGAGTCCATACTGCCCCTGATCCCAGAAGGAATGCTGACCCCTCGTCGT ATGAACTGTGCATAGTCTCCAGAGCTTCAAAGGCAACACAAGCTCGCAACTCTAAGATTTTTTTAAACCACAAAAACCCTGGTTAGCCATCTCATGCTCA GCCTTATCACTTCCCTCCCTTTAGAAACTCTCTCCCTGCTGTATATTAAAGGGAGCAGGTGGAGAGTCATTTTCCTTCGTCCTGCATGTCTCTAACATTA ATAGAAGGCATGGCTCCTGCTGCAACCGCTGTGAATGCTGCTGAGAACCTCCCTCTATGGGGATGGCTATTTTATTTTTGAGAAGGAAAAAAAAAGTCAT GTATATATACACATAAAGGCATATAGCTATATATAAAGAGATAAGGGTGTTTATGAAATGAGAAAATTATTGGACAATTCAGACTTTACTAAAGCACAGT TAGACCCAAGGCCTATGCTGAGGTCTAAACCTCTGAAAAAAGTATAGTATCGAGTACCCGTTCCCTCCCAGAGGTGGGAGTAACTGCTGGTAGTGCCTTC TTTGGTTGTGTTGCTCAGTGTGTAAGTGTTTGTTTCCAGGATATTTTCTTTTTAAATGTCTTTCTTATATGGGTTTTAAAAAAAAGTAATAAAAGCCTGT TGCAAAAA

refseq_RASSF5.F1 (forward primer)
refseq_RASSF5.R1 (reverse primer)
Single exon skipping

Frame 0

RE*RSRQSRRCQSCRH*PGMAMASPAIGQRPYP LLLDPEPPRYLQSLSGPELPPPPPDRSSRLCVPA PLSTAPGAREGRSARRAARGNLEPPPRASRPAR PLRPGLQQRLRRRPGAPRPRDVRSIFEQPQDPR VPAERGEGHCFAELVLPGGPGWCDLCGREVLRQA LRCTNCKFTCHPECRSLIQLDCSQQEGLSRDRP SPESTLTVTFSQNVCKPVEETQRPPTLQEIKQK IDSYNTREKNCLGMKLSEDGTYTGFIKVHLKLRR PVTVPAGIRPQSIYDAIKEVNLAATTDKRTSFY LPLDAIKQLHISSTTTVSEVIQGLLKKFMVVDN PQKFALFKRIHKDGQVLFQKLSIADRPLYLRLLA GPDTEVLSFVLKENETGEVEWDAFSIPELQNFL TILEKEEQDKIQQVQKKYDKFRQKLEEALRESQ GKPG*PVLLPLLLVHSDLFVLLIIILQQTLFLRT SLAGAFVPAQQFQLWQKSLPWTSVCMGVQLCPP PGCAPPQILPRIRTHVKQTADVLSRSASLSPTK *LPLSSPNWNLTPAGKGRKKGFRAVCSFSGFDSS LSSLTCHVQDHYL*VKSCSTFLLQV*AKPLKAG *CS*KDCSRGPKVPVVHT*GKFIKLLAPDAQGK EHQS*GWLRDLQRSCSLPWPWLWPWPTLHMETQR HISAYVWYLVTSLSTNCSFLSYKFEFNVVIIVM CFPKGKPVIDHLKSLLLSMEIRQ*EKAKKQCRE RCPSCLQPSPAKEQRRAWATWVPHRPPALPPSSH CDSGISRCCPRSCLDYREGSLQFGQLGVLSVLK HGPDPALRSESRWAWPPRRPQCLFVFREK*E*G EFA*SSAAGFSCQEVNNGGGVGDKARRARVQYGL RVTDLPPIWVPEF*LQSVIPDHIDREDQIPDLH LHWLLV*AES*NKLVKKFQQVARTAETLQCRGR RTCNFQSRAGFPTQPLRNHFFAILCLPKSLLGRF KPKLVRVASEVPSLTQAESILPLIPEGMLTPRR MNCA*SPELQRQHKLATLRFF*TTKTLVSHLML SLITSLPLETLSLLYIKGSRWRVIFLRPACL*H* *KAWLLLQPL*MLLRTSLYGDGYFIFEKEKKSH VYIHIKAYSYI*RDKGVYEMRKLLDNSDFTKAQ LDPRPMLRSKPLKKV*YRVPVPSQRWE*LLVVPS LVVLLSV*VFVSRIFSF*MSFLYGF*KKVIKAC CK

Frame 1

GSSAVAKAAAAKAAATSRAWPWRPRPSGSARTR YYWTPSRRAIYRA*AAPSYRRRPPTGPRASVSR RPSPLRPGRARGAAPGGLPGGTWSPRPGPPDPLA RSGLVCSRDCGGGLERPDPATCGASSSSRRIPE SRRSEARGTASPSWCCRAAPAGVTCADERCCGR RCAALTVNSPVTQNAAA*SSWTAVSRRVYPGTDP LQKAPSP*PSARMSVNLWRRHSARPHCRRSSRR STATTRERRTAWA*N*VKTAPTRVSSKCI*NSG GL*RCLLGSGPSPSMMPSRR*TWRLPRTSGHPST CP*MPSSSCTSAAPPPSVRSSRGCSRSSWLWTI PRSLHFLSGYTRTDKCSSRNSPLLTAPSTCACL LGLTRRSSALC*RRMKLER*SGMPSPSLNFRTS* QSWKKRSRTKSNKCKRSMTSLGRNWRRP*ENPR ANLGNRSCFLSSWCIQIYLYY*LLFCNRHFFSG HLWQVHLCLPSSSSCGKSLFHGQVFAWGFSCARP QAVPHHRFCQGSELM*NKQLTSSLDLQAFHQPN SCLSRHQTGTSHQPAKEGRKVLELCVLSLALIL L*VLLLATYRTIIYE*KVVAHSFCRSELSP*KQG NAHKRTVPAAPRCLLFTLKGSL*SYWPQMLRVR STKAEAGSEISREAAACPGPGSGPGPHCTWKPK GIYLRMCGT*SHLCQQTVRF*VTNLNLMLSSSSC VSPKGSQSLTI*KVSC*VWKSDSKRKPKSNAEK GVQAVFSLPQLKSRGGPGLLGFPIGLQHCLPPP TATLGSPGAAQGVALITERGASNSANLESFLF*S MGQTRHCARRAGGPGLPVDLSAFLFSERNRSRA SLPEALLLASPARK*TMAAVWETRPGEPAFSMG *GSQTSLPSGCLSFDSNQ*YQTTLTGRIKFLTYI CTGFLFRLNPKIN*SKNSNK*PGLQRHSSAEGE GLVIFKAGLVFQPSL*ETISLLSSAFPSPSWVG SSPSLFV*LQKFPL*PRLSPYCP*SQKEC*PLVV *TVHSLQSFKGNTSSQL*DFFKPQKPWLAISCS ALSLPSL*KLSPCCILKGAGGESFSFVLHVSNI NRRHGSCCNRCECC*EPPSMGMAILFLRRKKKVM YIYT*RHIAIYKEIRVFMK*ENYWTIQTLLKHS *TQGLC*GLNL*KKYSIEYPFPPRGGSNCW*CL LWLCCSVCKCLFPGYFLFKCLSYMGFKKK**KPV AK

Frame 2

GVAQSPKPPLPKLPPLAGHGHGVPGHRAAPVPA TIGPRAAALSTEPERPRATAAAPRPVLAPLCPG APLHCARGARGAQRPEGCPGEPGAPAPGLPTRS PAPAWSAAETAAAAWSAPTPRRAEHLRAAAGSQS PGGARRGALLRRVGAAGRPRLV*PVRTRGAAAG AALH*L*IHLSPRMPQPDPVGLQSAGGFIPGQT LSRKHPHRDLQPECL*TCGGDTAPAHTAGDQAED RQLQHAREELPGHETE*RRHLHGFHQSASETPA ACDGACWDPAPVHL*CHQGGEPGGYHGQADILL PAPRCHQAAAHQQHHHRQ*GHPGAAQEVHGCGQS PEVCTF*ADTQGRTSALPETLHC*PPPLPAPAC WA*HGGPQLCAKGE*NWRGRVGCLLHP*TSELP NNPGKRGAGQNPTSAKEV*QV*AETGGGLKRIPG QTWVTGPASSPPGAFRFICIINYYFATDTFSQD ISGRCICACPAVPAVAKVSSMDKCLHGGSAVPA PRLCPTTDSAKDQNSCETNS*RPLSICKPFTNQI VASLVTKLEPHTSRQRKEERF*SCVFFLWL*FF FEFSYLPRTGPLFMSEKL*HIPFAGLS*APESR VMLIKGLFPRPQGACCSHLREVYKATGPRCSG*G APKLRLAQRSPEKLQPALALALALAHIAHGNPK AYICVCVVLSHIFVNKLFVFKLQI*I*CCHHRH VFPQREASH*PFKKSPAKYGNQTVRESQKAMQRK VSKLSSAFPS*RAEEGLGYLGSPSASSTASLLP LRLWDLQVLPKELP*LQRGEPPIRPTWSPFCFE AWARPGTALGEPVGLASPSTSVPFCFQREIGVGR VCLKLCCWLLLPGSEQWRRCGRQGQESPRSVWV EGHRPPSHLGA*VLTPISDTRPH*QGGSNS*LT FALASCLG*ILK*ISQKIPTSSQDCRDTPVQREK DL*FSKQGWFSNPASEKPFLCYPLPSQVPLGSV QAQACSCSFRSSLSDPG*VHTAPDPRRNADPSS YELCIVSRASKATQARNSKIFLNHKNPG*PSHAQ PYHFPPFRNSLPAVY*REQVESHFPSSCMSLTL IEGMAPAATAVNAAENLPLWGWLFYF*EGKKKS CIYTHKGI*LYIKR*GCL*NEKIIGQFRLY*STV RPKAYAEV*TSEKSIVSSTRSLPEVGVTAGSAF FGCVAQCVSVCFQDIFFLNVFLIWVLKKSNKSL LQK