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Sequences

Sequence NM_002890

CCTCAGCCTGGGGAGCTGAAGGGGAGACGCGTCTGGGTGGGGCTGCTCGGAGCCCGGGCCTGGTGGCCCCTGGGGCTCCCGGGCGGGCAGGGTAGGGCAG AGTAGAGCGGGCTTCAACATGATGGCGGCCGAGGCCGGCAGTGAGGAGGGCGGCCCGGTAACAGCCGGAGCTGGAGGAGGCGGCGCGGCAGCGGGCTCCA GTGCCTATCCCGCAGTGTGTCGGGTGAAGATACCCGCGGCCCTGCCTGTGGCAGCCGCCCCCTATCCTGGGCTGGTGGAGACCGGAGTGGCTGGAACTCT GGGTGGCGGAGCCGCTTTGGGGTCAGAGTTCCTAGGAGCCGGGTCTGTGGCAGGGGCACTGGGGGGAGCTGGACTGACAGGGGGAGGTACTGCTGCTGGC GTAGCTGGTGCTGCTGCTGGCGTGGCCGGTGCTGCTGTTGCTGGACCTAGTGGAGACATGGCTCTCACCAAACTGCCCACTTCGTTGCTTGCTGAGACTC TCGGGCCAGGCGGCGGTTTTCCCCCTCTGCCCCCTCCCCCTTACCTGCCCCCTTTGGGGGCGGGCCTCGGGACAGTGGACGAAGGTGACTCTCTGGATGG ACCAGAATACGAGGAGGAAGAGGTGGCCATACCGTTGACCGCTCCTCCAACTAACCAGTGGTATCACGGAAAACTTGACAGAACGATAGCAGAAGAACGC CTCAGGCAGGCAGGGAAGTCTGGCAGTTATCTTATAAGAGAGAGTGATCGGAGGCCAGGGTCCTTTGTACTTTCATTTCTTAGCCAGATGAATGTTGTCA ACCATTTTAGGATTATTGCTATGTGTGGAGATTACTACATTGGTGGAAGACGTTTTTCTTCACTGTCAGACCTAATAGGTTATTACAGTCATGTTTCTTG TTTGCTTAAAGGAGAAAAATTACTTTACCCAGTTGCACCACCAGAGCCAGTAGAAGATAGAAGGCGTGTACGAGCTATTCTACCTTACACAAAAGTACCA GACACTGATGAAATAAGTTTCTTAAAAGGAGATATGTTCATTGTTCATAATGAATTAGAAGATGGATGGATGTGGGTTACAAATTTAAGAACAGATGAAC AAGGCCTTATTGTTGAAGACCTAGTAGAAGAGGTGGGCCGGGAAGAAGATCCACATGAAGGAAAAATATGGTTCCATGGGAAGATTTCCAAACAGGAAGC TTATAATTTACTAATGACAGTTGGTCAAGTCTGCAGTTTTCTTGTGAGGCCCTCAGATAATACTCCTGGCGATTATTCACTTTATTTCCGGACCAATGAA AATATTCAGCGATTTAAAATATGTCCAACGCCAAACAATCAGTTTATGATGGGAGGCCGGTATTATAACAGCATTGGGGACATCATAGATCACTATCGAA AAGAACAGATTGTTGAAGGATATTATCTTAAGGAACCTGTACCAATGCAGGATCAAGAACAAGTACTCAATGACACAGTGGATGGCAAGGAAATCTATAA TACCATCCGTCGTAAAACAAAGGATGCCTTTTATAAAAACATTGTTAAGAAAGGTTATCTTCTGAAAAAGGGCAAAGGAAAACGTTGGAAAAATTTATAT TTTATCTTAGAGGGTAGTGATGCCCAACTTATTTATTTTGAAAGCGAAAAACGAGCTACCAAACCAAAAGGATTAATAGATCTCAGTGTATGTTCTGTCT ATGTCGTTCATGATAGTCTCTTTGGCAGGCCAAACTGTTTTCAGATAGTAGTTCAGCACTTTAGTGAAGAACATTACATCTTTTACTTTGCAGGAGAAAC TCCAGAACAAGCAGAGGATTGGATGAAAGGTCTGCAGGCATTTTGCAATTTACGGAAAAGTAGTCCAGGGACATCCAATAAACGCCTTCGTCAGGTCAGC AGCCTTGTTTTACATATTGAAGAAGCCCATAAACTCCCAGTAAAACATTTTACTAATCCATATTGTAACATCTACCTGAATAGTGTCCAAGTAGCAAAAA CTCATGCAAGGGAAGGGCAAAACCCAGTATGGTCAGAAGAGTTTGTCTTTGATGATCTTCCTCCTGACATCAATAGATTTGAAATAACTCTTAGTAATAA AACAAAGAAAAGCAAAGATCCTGATATCTTATTTATGCGCTGCCAGTTGAGCCGATTACAGAAAGGGCATGCCACAGATGAATGGTTTCTGCTCAGCTCC CATATACCATTAAAAGGTATTGAACCAGGGTCCCTGCGTGTTCGAGCACGATACTCTATGGAAAAAATCATGCCAGAAGAAGAGTACAGTGAATTTAAAG AGCTTATACTGCAAAAGGAACTTCATGTAGTCTATGCTTTATCACATGTATGTGGACAAGACCGAACACTACTGGCCAGCATCCTACTGAGGATTTTTCT TCACGAAAAGCTTGAATCGTTGTTGTTATGCACACTAAATGACAGAGAAATAAGCATGGAAGATGAAGCCACTACCCTATTTCGAGCCACAACACTTGCA AGCACCTTGATGGAGCAGTATATGAAAGCCACTGCTACACAGTTTGTTCATCATGCTTTGAAAGACTCTATTTTAAAGATAATGGAAAGCAAGCAGTCTT GTGAGTTAAGTCCATCAAAGTTAGAAAAAAATGAAGATGTGAACACTAATTTAACACACCTATTGAACATACTTTCAGAGCTTGTGGAGAAAATATTCAT GGCTTCAGAAATACTTCCACCGACATTGAGATATATTTATGGGTGTTTACAGAAATCTGTTCAGCATAAGTGGCCTACAAATACCACCATGAGAACAAGA GTTGTTAGTGGTTTTGTTTTTCTTCGACTCATCTGTCCTGCCATCCTGAATCCACGGATGTTCAATATCATCTCAGATTCTCCATCTCCTATTGCTGCAA GAACACTGATATTAGTGGCTAAATCTGTGCAGAACTTAGCAAATCTTGTGGAATTTGGAGCTAAGGAGCCCTACATGGAAGGTGTCAATCCATTCATCAA AAGCAACAAACATCGTATGATCATGTTTTTAGATGAACTTGGGAATGTACCTGAACTTCCGGACACTACAGAGCATTCTAGAACGGACCTGTCCCGTGAT TTAGCAGCATTGCATGAGATTTGCGTGGCTCATTCAGATGAACTTCGAACGCTCAGTAATGAGCGTGGTGCACAGCAGCACGTATTGAAAAAGCTTCTGG CTATAACAGAACTGCTTCAACAAAAACAAAACCAGTATACAAAAACCAATGATGTCAGGTAGCAGCCTTCGCCCCAGTGTTCTGCATGGATTCAGCATGT CCAACATGGTAATTCACTTCAGTTTAATGTCTCCTTTGCTCTTGCCAAAAAATAGCACACTTTTCCACATTCCAGTGATGTGTGAGCTATGCAAACAAAA TCCAAGATTCTGCTGGTGAATAACTATGCCAGCAACCTTGTAAGCTATCTGTGCAGGATATTTGCACTATTTCCACATGGAATCAATCTTTAACAACCTC TGAGCCTTGGTGTACAGACCACCTTTCACAAAACGAAATGCTATGACTGTATCTTGATATCTCGAACTTTCAAAATATATTTTCAGTACACCCAGTTGCC AAAGTTTTGCTGTCTCTTAGAGAAAGAACTATGAAATCAACTGACAAGAAACACATTCTTATTGACAATTGTGTATAACTGGATTGCAGACTGTTCTTAC TGTAACTACTTCCTGATTAGGAATATGACCATTTGACTGTTCAATGATTATTTGTATTTACAGTTTCCAGAGTTTGTCATTATAATAGGAACAATCTTTG CTGTATACTTTTAAAAAATACTCTGCTATTTCTCTTGCTGGAACTGTTGAAAGAAAATATATAGAATGATCTATTGCTCATCAGCTTTATTTTTTAAACA TACGACTTATTTTGTTGAAATTGTCAAAGACTGTATTTAGATCTCATAATGCTTTGTTAAATGTTTACAAGTAAATAGTTTGAATTCAGTAAATATTATT GGTTGTTGTATTGATCAATGCATGTTACCCATTCAACCATTTTATAGACTACCAATTTCTTTTATGTTAACTAGAATGCTTTTGTTAAAAGTTATTTGTT CATTATTTGTGCTACCCCTTTGATTATGCAGACAACCTCATCAGCTGCCTAACTTATCCATCTTTGAACTTCTGACTACTTGTTGTATCTGCTGGATATT TAGTTCAACTGTATAGTTTTATTTACTTCTGTATGTGTATTTTTGTGAAGTATTCACAAAGGTTAAGTTAAAATAAAACCAAGGGATATCTTGCA

refseq_RASA1.F1 (forward primer)
refseq_RASA1.R1 (reverse primer)
Alternative donor

Frame 0

PQPGELKGRRVWVGLLGARAWWPLGLPGGQGRA E*SGLQHDGGRGRQ*GGRPGNSRSWRRRRGSGLQ CLSRSVSGEDTRGPACGSRPLSWAGGDRSGWNS GWRSRFGVRVPRSRVCGRGTGGSWTDRGRYCCW RSWCCCWRGRCCCCWT*WRHGSHQTAHFVAC*DS RARRRFSPSAPSPLPAPFGGGPRDSGRR*LSGW TRIRGGRGGHTVDRSSN*PVVSRKT*QNDSRRT PQAGREVWQLSYKRE*SEARVLCTFIS*PDECCQ PF*DYCYVWRLLHWWKTFFFTVRPNRLLQSCFL FA*RRKITLPSCTTRASRR*KACTSYSTLHKST RH**NKFLKRRYVHCS**IRRWMDVGYKFKNR*T RPYC*RPSRRGGPGRRST*RKNMVPWEDFQTGS L*FTNDSWSSLQFSCEALR*YSWRLFTLFPDQ* KYSAI*NMSNAKQSVYDGRPVL*QHWGHHRSLSK RTDC*RILS*GTCTNAGSRTSTQ*HSGWQGNL* YHPS*NKGCLL*KHC*ERLSSEKGQRKTLEKFI FYLRG**CPTYLF*KRKTSYQTKRINRSQCMFCL CRS**SLWQAKLFSDSSSAL**RTLHLLLCRRN SRTSRGLDERSAGILQFTEK*SRDIQ*TPSSGQ QPCFTY*RSP*TPSKTFY*SIL*HLPE*CPSSKN SCKGRAKPSMVRRVCL**SSS*HQ*I*NNS*** NKEKQRS*YLIYALPVEPITERACHR*MVSAQL PYTIKRY*TRVPACSSTILYGKNHARRRVQ*I*R AYTAKGTSCSLCFITCMWTRPNTTGQHPTEDFS SRKA*IVVVMHTK*QRNKHGR*SHYPISSHNTC KHLDGAVYESHCYTVCSSCFERLYFKDNGKQAVL *VKSIKVRKK*RCEH*FNTPIEHTFRACGENIH GFRNTSTDIEIYLWVFTEICSA*VAYKYHHENK SC*WFCFSSTHLSCHPESTDVQYHLRFSISYCCK NTDISG*ICAELSKSCGIWS*GALHGRCQSIHQ KQQTSYDHVFR*TWECT*TSGHYRAF*NGPVP* FSSIA*DLRGSFR*TSNAQ**AWCTAARIEKASG YNRTASTKTKPVYKNQ*CQVAAFAPVFCMDSAC PTW*FTSV*CLLCSCQKIAHFSTFQ*CVSYANK IQDSAGE*LCQQPCKLSVQDICTISTWNQSLTTS EPWCTDHLSQNEML*LYLDISNFQNIFSVHPVA KVLLSLRERTMKSTDKKHILIDNCV*LDCRLFL L*LLPD*EYDHLTVQ*LFVFTVSRVCHYNRNNLC CILLKNTLLFLLLELLKENI*NDLLLISFIF*T YDLFC*NCQRLYLDLIMLC*MFTSK*FEFSKYY WLLY*SMHVTHSTIL*TTNFFYVN*NAFVKSYLF IICATPLIMQTTSSAA*LIHL*TSDYLLYLLDI *FNCIVLFTSVCVFL*SIHKG*VKIKPRDIL

Frame 1

LSLGS*RGDASGWGCSEPGPGGPWGSRAGRVGQ SRAGFNMMAAEAGSEEGGPVTAGAGGGGAAAGS SAYPAVCRVKIPAALPVAAAPYPGLVETGVAGTL GGGAALGSEFLGAGSVAGALGGAGLTGGGTAAG VAGAAAGVAGAAVAGPSGDMALTKLPTSLLAET LGPGGGFPPLPPPPYLPPLGAGLGTVDEGDSLDG PEYEEEEVAIPLTAPPTNQWYHGKLDRTIAEER LRQAGKSGSYLIRESDRRPGSFVLSFLSQMNVV NHFRIIAMCGDYYIGGRRFSSLSDLIGYYSHVSC LLKGEKLLYPVAPPEPVEDRRRVRAILPYTKVP DTDEISFLKGDMFIVHNELEDGWMWVTNLRTDE QGLIVEDLVEEVGREEDPHEGKIWFHGKISKQEA YNLLMTVGQVCSFLVRPSDNTPGDYSLYFRTNE NIQRFKICPTPNNQFMMGGRYYNSIGDIIDHYR KEQIVEGYYLKEPVPMQDQEQVLNDTVDGKEIYN TIRRKTKDAFYKNIVKKGYLLKKGKGKRWKNLY FILEGSDAQLIYFESEKRATKPKGLIDLSVCSV YVVHDSLFGRPNCFQIVVQHFSEEHYIFYFAGET PEQAEDWMKGLQAFCNLRKSSPGTSNKRLRQVS SLVLHIEEAHKLPVKHFTNPYCNIYLNSVQVAK THAREGQNPVWSEEFVFDDLPPDINRFEITLSNK TKKSKDPDILFMRCQLSRLQKGHATDEWFLLSS HIPLKGIEPGSLRVRARYSMEKIMPEEEYSEFK ELILQKELHVVYALSHVCGQDRTLLASILLRIFL HEKLESLLLCTLNDREISMEDEATTLFRATTLA STLMEQYMKATATQFVHHALKDSILKIMESKQS CELSPSKLEKNEDVNTNLTHLLNILSELVEKIFM ASEILPPTLRYIYGCLQKSVQHKWPTNTTMRTR VVSGFVFLRLICPAILNPRMFNIISDSPSPIAA RTLILVAKSVQNLANLVEFGAKEPYMEGVNPFIK SNKHRMIMFLDELGNVPELPDTTEHSRTDLSRD LAALHEICVAHSDELRTLSNERGAQQHVLKKLL AITELLQQKQNQYTKTNDVR*QPSPQCSAWIQHV QHGNSLQFNVSFALAKK*HTFPHSSDV*AMQTK SKILLVNNYASNLVSYLCRIFALFPHGINL*QP LSLGVQTTFHKTKCYDCILISRTFKIYFQYTQLP KFCCLLEKEL*NQLTRNTFLLTIVYNWIADCSY CNYFLIRNMTI*LFNDYLYLQFPEFVIIIGTIF AVYF*KILCYFSCWNC*KKIYRMIYCSSALFFKH TTYFVEIVKDCI*IS*CFVKCLQVNSLNSVNII GCCIDQCMLPIQPFYRLPISFMLTRMLLLKVIC SLFVLPL*LCRQPHQLPNLSIFELLTTCCICWIF SSTV*FYLLLYVYFCEVFTKVKLK*NQGISC

Frame 2

SAWGAEGETRLGGAARSPGLVAPGAPGRAG*GR VERAST*WRPRPAVRRAAR*QPELEEAARQRAP VPIPQCVG*RYPRPCLWQPPPILGWWRPEWLEL WVAEPLWGQSS*EPGLWQGHWGELD*QGEVLLLA *LVLLLAWPVLLLLDLVETWLSPNCPLRCLLRL SGQAAVFPLCPLPLTCPLWGRASGQWTKVTLWM DQNTRRKRWPYR*PLLQLTSGITENLTER*QKNA SGRQGSLAVIL*ERVIGGQGPLYFHFLAR*MLS TILGLLLCVEITTLVEDVFLHCQT**VITVMFL VCLKEKNYFTQLHHQSQ*KIEGVYELFYLTQKYQ TLMK*VS*KEICSLFIMN*KMDGCGLQI*EQMN KALLLKT**KRWAGKKIHMKEKYGSMGRFPNRK LIIY**QLVKSAVFL*GPQIILLAIIHFISGPMK IFSDLKYVQRQTISL*WEAGIITALGTS*ITIE KNRLLKDIILRNLYQCRIKNKYSMTQWMARKSI IPSVVKQRMPFIKTLLRKVIF*KRAKENVGKIYI LS*RVVMPNLFILKAKNELPNQKD**ISVYVLS MSFMIVSLAGQTVFR**FSTLVKNITSFTLQEK LQNKQRIG*KVCRHFAIYGKVVQGHPINAFVRSA ALFYILKKPINSQ*NILLIHIVTST*IVSK*QK LMQGKGKTQYGQKSLSLMIFLLTSIDLK*LLVI KQRKAKILISYLCAAS*ADYRKGMPQMNGFCSAP IYH*KVLNQGPCVFEHDTLWKKSCQKKSTVNLK SLYCKRNFM*SMLYHMYVDKTEHYWPASY*GFF FTKSLNRCCYAH*MTEK*AWKMKPLPYFEPQHLQ AP*WSSI*KPLLHSLFIML*KTLF*R*WKASSL VS*VHQS*KKMKM*TLI*HTY*TYFQSLWRKYS WLQKYFHRH*DIFMGVYRNLFSISGLQIPP*EQE LLVVLFFFDSSVLPS*IHGCSISSQILHLLLLQ EH*Y*WLNLCRT*QILWNLELRSPTWKVSIHSS KATNIV*SCF*MNLGMYLNFRTLQSILERTCPVI *QHCMRFAWLIQMNFERSVMSVVHSSTY*KSFW L*QNCFNKNKTSIQKPMMSGSSLRPSVLHGFSM SNMVIHFSLMSPLLLPKNSTLFHIPVMCELCKQN PRFCW*ITMPATL*AICAGYLHYFHMESIFNNL *ALVYRPPFTKRNAMTVS*YLELSKYIFSTPSC QSFAVS*RKNYEIN*QETHSY*QLCITGLQTVLT VTTS*LGI*PFDCSMIICIYSFQSLSL**EQSL LYTFKKYSAISLAGTVERKYIE*SIAHQLYFLN IRLILLKLSKTVFRSHNALLNVYK*IV*IQ*ILL VVVLINACYPFNHFIDYQFLLC*LECFC*KLFV HYLCYPFDYADNLISCLTYPSLNF*LLVVSAGY LVQLYSFIYFCMCIFVKYSQRLS*NKTKGYLA

Sequence NM_022650

GGAAGAGGTGGCCATACCGTTGACCGCTCCTCCAACTAACCAGTAAGTTAAGACTGCTGTTCAGGAATTTGGGAAGCTGGCTCCAGAAAAGAAGTGGAAA TGAAGGGGTGGTATCACGGAAAACTTGACAGAACGATAGCAGAAGAACGCCTCAGGCAGGCAGGGAAGTCTGGCAGTTATCTTATAAGAGAGAGTGATCG GAGGCCAGGGTCCTTTGTACTTTCATTTCTTAGCCAGATGAATGTTGTCAACCATTTTAGGATTATTGCTATGTGTGGAGATTACTACATTGGTGGAAGA CGTTTTTCTTCACTGTCAGACCTAATAGGTTATTACAGTCATGTTTCTTGTTTGCTTAAAGGAGAAAAATTACTTTACCCAGTTGCACCACCAGAGCCAG TAGAAGATAGAAGGCGTGTACGAGCTATTCTACCTTACACAAAAGTACCAGACACTGATGAAATAAGTTTCTTAAAAGGAGATATGTTCATTGTTCATAA TGAATTAGAAGATGGATGGATGTGGGTTACAAATTTAAGAACAGATGAACAAGGCCTTATTGTTGAAGACCTAGTAGAAGAGGTGGGCCGGGAAGAAGAT CCACATGAAGGAAAAATATGGTTCCATGGGAAGATTTCCAAACAGGAAGCTTATAATTTACTAATGACAGTTGGTCAAGTCTGCAGTTTTCTTGTGAGGC CCTCAGATAATACTCCTGGCGATTATTCACTTTATTTCCGGACCAATGAAAATATTCAGCGATTTAAAATATGTCCAACGCCAAACAATCAGTTTATGAT GGGAGGCCGGTATTATAACAGCATTGGGGACATCATAGATCACTATCGAAAAGAACAGATTGTTGAAGGATATTATCTTAAGGAACCTGTACCAATGCAG GATCAAGAACAAGTACTCAATGACACAGTGGATGGCAAGGAAATCTATAATACCATCCGTCGTAAAACAAAGGATGCCTTTTATAAAAACATTGTTAAGA AAGGTTATCTTCTGAAAAAGGGCAAAGGAAAACGTTGGAAAAATTTATATTTTATCTTAGAGGGTAGTGATGCCCAACTTATTTATTTTGAAAGCGAAAA ACGAGCTACCAAACCAAAAGGATTAATAGATCTCAGTGTATGTTCTGTCTATGTCGTTCATGATAGTCTCTTTGGCAGGCCAAACTGTTTTCAGATAGTA GTTCAGCACTTTAGTGAAGAACATTACATCTTTTACTTTGCAGGAGAAACTCCAGAACAAGCAGAGGATTGGATGAAAGGTCTGCAGGCATTTTGCAATT TACGGAAAAGTAGTCCAGGGACATCCAATAAACGCCTTCGTCAGGTCAGCAGCCTTGTTTTACATATTGAAGAAGCCCATAAACTCCCAGTAAAACATTT TACTAATCCATATTGTAACATCTACCTGAATAGTGTCCAAGTAGCAAAAACTCATGCAAGGGAAGGGCAAAACCCAGTATGGTCAGAAGAGTTTGTCTTT GATGATCTTCCTCCTGACATCAATAGATTTGAAATAACTCTTAGTAATAAAACAAAGAAAAGCAAAGATCCTGATATCTTATTTATGCGCTGCCAGTTGA GCCGATTACAGAAAGGGCATGCCACAGATGAATGGTTTCTGCTCAGCTCCCATATACCATTAAAAGGTATTGAACCAGGGTCCCTGCGTGTTCGAGCACG ATACTCTATGGAAAAAATCATGCCAGAAGAAGAGTACAGTGAATTTAAAGAGCTTATACTGCAAAAGGAACTTCATGTAGTCTATGCTTTATCACATGTA TGTGGACAAGACCGAACACTACTGGCCAGCATCCTACTGAGGATTTTTCTTCACGAAAAGCTTGAATCGTTGTTGTTATGCACACTAAATGACAGAGAAA TAAGCATGGAAGATGAAGCCACTACCCTATTTCGAGCCACAACACTTGCAAGCACCTTGATGGAGCAGTATATGAAAGCCACTGCTACACAGTTTGTTCA TCATGCTTTGAAAGACTCTATTTTAAAGATAATGGAAAGCAAGCAGTCTTGTGAGTTAAGTCCATCAAAGTTAGAAAAAAATGAAGATGTGAACACTAAT TTAACACACCTATTGAACATACTTTCAGAGCTTGTGGAGAAAATATTCATGGCTTCAGAAATACTTCCACCGACATTGAGATATATTTATGGGTGTTTAC AGAAATCTGTTCAGCATAAGTGGCCTACAAATACCACCATGAGAACAAGAGTTGTTAGTGGTTTTGTTTTTCTTCGACTCATCTGTCCTGCCATCCTGAA TCCACGGATGTTCAATATCATCTCAGATTCTCCATCTCCTATTGCTGCAAGAACACTGATATTAGTGGCTAAATCTGTGCAGAACTTAGCAAATCTTGTG GAATTTGGAGCTAAGGAGCCCTACATGGAAGGTGTCAATCCATTCATCAAAAGCAACAAACATCGTATGATCATGTTTTTAGATGAACTTGGGAATGTAC CTGAACTTCCGGACACTACAGAGCATTCTAGAACGGACCTGTCCCGTGATTTAGCAGCATTGCATGAGATTTGCGTGGCTCATTCAGATGAACTTCGAAC GCTCAGTAATGAGCGTGGTGCACAGCAGCACGTATTGAAAAAGCTTCTGGCTATAACAGAACTGCTTCAACAAAAACAAAACCAGTATACAAAAACCAAT GATGTCAGGTAGCAGCCTTCGCCCCAGTGTTCTGCATGGATTCAGCATGTCCAACATGGTAATTCACTTCAGTTTAATGTCTCCTTTGCTCTTGCCAAAA AATAGCACACTTTTCCACATTCCAGTGATGTGTGAGCTATGCAAACAAAATCCAAGATTCTGCTGGTGAATAACTATGCCAGCAACCTTGTAAGCTATCT GTGCAGGATATTTGCACTATTTCCACATGGAATCAATCTTTAACAACCTCTGAGCCTTGGTGTACAGACCACCTTTCACAAAACGAAATGCTATGACTGT ATCTTGATATCTCGAACTTTCAAAATATATTTTCAGTACACCCAGTTGCCAAAGTTTTGCTGTCTCTTAGAGAAAGAACTATGAAATCAACTGACAAGAA ACACATTCTTATTGACAATTGTGTATAACTGGATTGCAGACTGTTCTTACTGTAACTACTTCCTGATTAGGAATATGACCATTTGACTGTTCAATGATTA TTTGTATTTACAGTTTCCAGAGTTTGTCATTATAATAGGAACAATCTTTGCTGTATACTTTTAAAAAATACTCTGCTATTTCTCTTGCTGGAACTGTTGA AAGAAAATATATAGAATGATCTATTGCTCATCAGCTTTATTTTTTAAACATACGACTTATTTTGTTGAAATTGTCAAAGACTGTATTTAGATCTCATAAT GCTTTGTTAAATGTTTACAAGTAAATAGTTTGAATTCAGTAAATATTATTGGTTGTTGTATTGATCAATGCATGTTACCCATTCAACCATTTTATAGACT ACCAATTTCTTTTATGTTAACTAGAATGCTTTTGTTAAAAGTTATTTGTTCATTATTTGTGCTACCCCTTTGATTATGCAGACAACCTCATCAGCTGCCT AACTTATCCATCTTTGAACTTCTGACTACTTGTTGTATCTGCTGGATATTTAGTTCAACTGTATAGTTTTATTTACTTCTGTATGTGTATTTTTGTGAAG TATTCACAAAGGTTAAGTTAAAATAAAACCAAGGGATATCTTGCA

refseq_RASA1.F1 (forward primer)
refseq_RASA1.R1 (reverse primer)
Alternative donor

Frame 0

GRGGHTVDRSSN*PVS*DCCSGIWEAGSRKEVE MKGWYHGKLDRTIAEERLRQAGKSGSYLIRESDR RPGSFVLSFLSQMNVVNHFRIIAMCGDYYIGGR RFSSLSDLIGYYSHVSCLLKGEKLLYPVAPPEP VEDRRRVRAILPYTKVPDTDEISFLKGDMFIVHN ELEDGWMWVTNLRTDEQGLIVEDLVEEVGREED PHEGKIWFHGKISKQEAYNLLMTVGQVCSFLVR PSDNTPGDYSLYFRTNENIQRFKICPTPNNQFMM GGRYYNSIGDIIDHYRKEQIVEGYYLKEPVPMQ DQEQVLNDTVDGKEIYNTIRRKTKDAFYKNIVK KGYLLKKGKGKRWKNLYFILEGSDAQLIYFESEK RATKPKGLIDLSVCSVYVVHDSLFGRPNCFQIV VQHFSEEHYIFYFAGETPEQAEDWMKGLQAFCN LRKSSPGTSNKRLRQVSSLVLHIEEAHKLPVKHF TNPYCNIYLNSVQVAKTHAREGQNPVWSEEFVF DDLPPDINRFEITLSNKTKKSKDPDILFMRCQL SRLQKGHATDEWFLLSSHIPLKGIEPGSLRVRAR YSMEKIMPEEEYSEFKELILQKELHVVYALSHV CGQDRTLLASILLRIFLHEKLESLLLCTLNDRE ISMEDEATTLFRATTLASTLMEQYMKATATQFVH HALKDSILKIMESKQSCELSPSKLEKNEDVNTN LTHLLNILSELVEKIFMASEILPPTLRYIYGCL QKSVQHKWPTNTTMRTRVVSGFVFLRLICPAILN PRMFNIISDSPSPIAARTLILVAKSVQNLANLV EFGAKEPYMEGVNPFIKSNKHRMIMFLDELGNV PELPDTTEHSRTDLSRDLAALHEICVAHSDELRT LSNERGAQQHVLKKLLAITELLQQKQNQYTKTN DVR*QPSPQCSAWIQHVQHGNSLQFNVSFALAK K*HTFPHSSDV*AMQTKSKILLVNNYASNLVSYL CRIFALFPHGINL*QPLSLGVQTTFHKTKCYDC ILISRTFKIYFQYTQLPKFCCLLEKEL*NQLTR NTFLLTIVYNWIADCSYCNYFLIRNMTI*LFNDY LYLQFPEFVIIIGTIFAVYF*KILCYFSCWNC* KKIYRMIYCSSALFFKHTTYFVEIVKDCI*IS* CFVKCLQVNSLNSVNIIGCCIDQCMLPIQPFYRL PISFMLTRMLLLKVICSLFVLPL*LCRQPHQLP NLSIFELLTTCCICWIFSSTV*FYLLLYVYFCE VFTKVKLK*NQGISC

Frame 1

EEVAIPLTAPPTNQ*VKTAVQEFGKLAPEKKWK *RGGITENLTER*QKNASGRQGSLAVIL*ERVI GGQGPLYFHFLAR*MLSTILGLLLCVEITTLVED VFLHCQT**VITVMFLVCLKEKNYFTQLHHQSQ *KIEGVYELFYLTQKYQTLMK*VS*KEICSLFI MN*KMDGCGLQI*EQMNKALLLKT**KRWAGKKI HMKEKYGSMGRFPNRKLIIY**QLVKSAVFL*G PQIILLAIIHFISGPMKIFSDLKYVQRQTISL* WEAGIITALGTS*ITIEKNRLLKDIILRNLYQCR IKNKYSMTQWMARKSIIPSVVKQRMPFIKTLLR KVIF*KRAKENVGKIYILS*RVVMPNLFILKAK NELPNQKD**ISVYVLSMSFMIVSLAGQTVFR** FSTLVKNITSFTLQEKLQNKQRIG*KVCRHFAI YGKVVQGHPINAFVRSAALFYILKKPINSQ*NI LLIHIVTST*IVSK*QKLMQGKGKTQYGQKSLSL MIFLLTSIDLK*LLVIKQRKAKILISYLCAAS* ADYRKGMPQMNGFCSAPIYH*KVLNQGPCVFEH DTLWKKSCQKKSTVNLKSLYCKRNFM*SMLYHMY VDKTEHYWPASY*GFFFTKSLNRCCYAH*MTEK *AWKMKPLPYFEPQHLQAP*WSSI*KPLLHSLF IML*KTLF*R*WKASSLVS*VHQS*KKMKM*TLI *HTY*TYFQSLWRKYSWLQKYFHRH*DIFMGVY RNLFSISGLQIPP*EQELLVVLFFFDSSVLPS* IHGCSISSQILHLLLLQEH*Y*WLNLCRT*QILW NLELRSPTWKVSIHSSKATNIV*SCF*MNLGMY LNFRTLQSILERTCPVI*QHCMRFAWLIQMNFE RSVMSVVHSSTY*KSFWL*QNCFNKNKTSIQKPM MSGSSLRPSVLHGFSMSNMVIHFSLMSPLLLPK NSTLFHIPVMCELCKQNPRFCW*ITMPATL*AI CAGYLHYFHMESIFNNL*ALVYRPPFTKRNAMTV S*YLELSKYIFSTPSCQSFAVS*RKNYEIN*QE THSY*QLCITGLQTVLTVTTS*LGI*PFDCSMI ICIYSFQSLSL**EQSLLYTFKKYSAISLAGTVE RKYIE*SIAHQLYFLNIRLILLKLSKTVFRSHN ALLNVYK*IV*IQ*ILLVVVLINACYPFNHFID YQFLLC*LECFC*KLFVHYLCYPFDYADNLISCL TYPSLNF*LLVVSAGYLVQLYSFIYFCMCIFVK YSQRLS*NKTKGYLA

Frame 2

KRWPYR*PLLQLTSKLRLLFRNLGSWLQKRSGN EGVVSRKT*QNDSRRTPQAGREVWQLSYKRE*S EARVLCTFIS*PDECCQPF*DYCYVWRLLHWWK TFFFTVRPNRLLQSCFLFA*RRKITLPSCTTRAS RR*KACTSYSTLHKSTRH**NKFLKRRYVHCS* *IRRWMDVGYKFKNR*TRPYC*RPSRRGGPGRR ST*RKNMVPWEDFQTGSL*FTNDSWSSLQFSCEA LR*YSWRLFTLFPDQ*KYSAI*NMSNAKQSVYD GRPVL*QHWGHHRSLSKRTDC*RILS*GTCTNA GSRTSTQ*HSGWQGNL*YHPS*NKGCLL*KHC*E RLSSEKGQRKTLEKFIFYLRG**CPTYLF*KRK TSYQTKRINRSQCMFCLCRS**SLWQAKLFSDS SSAL**RTLHLLLCRRNSRTSRGLDERSAGILQF TEK*SRDIQ*TPSSGQQPCFTY*RSP*TPSKTF Y*SIL*HLPE*CPSSKNSCKGRAKPSMVRRVCL **SSS*HQ*I*NNS***NKEKQRS*YLIYALPVE PITERACHR*MVSAQLPYTIKRY*TRVPACSST ILYGKNHARRRVQ*I*RAYTAKGTSCSLCFITC MWTRPNTTGQHPTEDFSSRKA*IVVVMHTK*QRN KHGR*SHYPISSHNTCKHLDGAVYESHCYTVCS SCFERLYFKDNGKQAVL*VKSIKVRKK*RCEH* FNTPIEHTFRACGENIHGFRNTSTDIEIYLWVFT EICSA*VAYKYHHENKSC*WFCFSSTHLSCHPE STDVQYHLRFSISYCCKNTDISG*ICAELSKSC GIWS*GALHGRCQSIHQKQQTSYDHVFR*TWECT *TSGHYRAF*NGPVP*FSSIA*DLRGSFR*TSN AQ**AWCTAARIEKASGYNRTASTKTKPVYKNQ *CQVAAFAPVFCMDSACPTW*FTSV*CLLCSCQK IAHFSTFQ*CVSYANKIQDSAGE*LCQQPCKLS VQDICTISTWNQSLTTSEPWCTDHLSQNEML*L YLDISNFQNIFSVHPVAKVLLSLRERTMKSTDKK HILIDNCV*LDCRLFLL*LLPD*EYDHLTVQ*L FVFTVSRVCHYNRNNLCCILLKNTLLFLLLELL KENI*NDLLLISFIF*TYDLFC*NCQRLYLDLIM LC*MFTSK*FEFSKYYWLLY*SMHVTHSTIL*T TNFFYVN*NAFVKSYLFIICATPLIMQTTSSAA *LIHL*TSDYLLYLLDI*FNCIVLFTSVCVFL*S IHKG*VKIKPRDIL