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Sequence NM_031992
GACGGCAAATGGCGGACTTCGACACCTACGACGATCGGGCCTACAGCAGCTTCGGCGGCGGCAGAGGGTCCCGCGGCAGTGCTGGTGGCCATGGTTCCCGTAGCCAGAAGGAGTTGCCCACAGAGCCCCCCTACACAGCATACGTAGGAAATCTACCTTTCAATACGGTTCAGGGCGACATAGATGCTATCTTTAAGGAT
CTCAGCATAAGGAGTGTACGGCTAGTCAGAGACAAAGACACAGATAAATTTAAAGGATTCTGCTATGTAGAATTCGATGAAGTGGATTCCCTTAAGGAAG
CCTTGACATACGATGGTGCACTGTTGGGCGATCGGTCACTTCGTGTGGACATTGCAGAAGGCAGAAAACAAGATAAAGGTGGCTTTGGATTCAGAAAAGG
TGGACCAGATGACAGAGGCTTCAGGGATGACTTCTTAGGGGGCAGGGGAGGTAGTCGCCCAGGCGACCGGCGAACAGGCCCCCCCATGGGCAGCCGCTTC
AGAGATGGCCCTCCCCTCCGTGGATCCAACATGGATTTCAGAGAACCCACAGAAGAGGAAAGAGCACAGAGACCACGACTCCAGCTTAAACCTCGAACAG
TCGCGACGCCCCTCAATCAAGTAGCCAATCCCAACTCTGCTATCTTCGGGGGTGCCAGGCCTAGAGAGGAAGTCGTTCAAAAGGAGCAAGAATGAGCCTG
CGGTTGGGAGGGAATGGGGCGTGGGGGGTTAGAGCAGGACCACAGCCTGGTGAGTCCCCGGGCAGCCGTCCTGCAGCCGCCACTCCTGCGCCTGCCATTG
GCCTCCTCACAGCGGAAACACAGCTTGTGAGTGCATGTCAGCTGTTAACAAGTGGTTTTTAGTACATTCTGGGCTTTGCTGTATCTATCTAGTGCCTGTT
TGTGCGTTTTTTTCTTTCTTCCGCTGCTTCCCCATTTTCCTTCTGTCCTTTTTCTCCTGCTCCTTGTTTTCCCAGCAGCACATGGGGTTCCTCGGAGGAG
CAGAGGTGGCCGCCGTGGGGGGGCGTTTGGGCTGCGGTGCTGCGTCATTTTTCCTTTGCTTTCTCTTTACTTTAGACACTGGCCCAACTCCAGGCGTTTC
CTTTCATTCCCTCAGTGCTTCTCTTCTGACCTGCATGTTGAGTTCTGTATTGCTGGGGCTTCCAACAAAAACCAGAGTCACTGACAGAGGGAACAGCAGA
GACCTTGTTGGTATTCAGCTGTGATGGATATAGAGAATCAGAGGCACCTTGTTTTCACAACTAGGATAAAAATATCTGCAGGGTCCTTTCCATTCCTATT
TAGAGGGAGTCCTGGCTCCATGACCCCCTCCCGAGTGGACTGTCCAAGCAGATAGGCTCACACGAGAAACAGTGAGGCTGAAAGGGGGGGCTATGGAAGA
GCGGTAGGGAGTCCACGGAGAAGATGCAGTGAATGCTTGCATGCATTCACACGTGTGTGTGTCCCAGCTAGTTCACTCCTTTCGCCGTGCGTGGTGGAGG
CTGGCCTCTCTGGCTGGGTGCAGTGAATGGCCAGCGGGTTTCTTTTCTGCTGGGCCAAGGCGCTTTGGGGGTGGAGGGGGTGGTGCTGGTGCTGCACTGG
GCTGACTGCGGCGCTGACGCAGCGTTTCCCCCCATCCCTGTTGCCTGTGTGTTGTGTGGATCTGTTCCTAGTATAGGCAACATAATGAGATACTGTGCTT
CCCACCTCCCCTTCAGTTCAGAGCCAAAATGGGTCTAGAATCTGGCACTTTACTCATTTCCTTTGATAAATTGTACTATGCAGAGCTGTCAGGAACCTTC
AGATAGCAGTAGAGGACTGCAGCTGTCTAGGTCTGCGGCCACATCTTGGGGACACACTGGACTGTTCCCATGTGCAGGGTTCAGCAGTTATGTGGGAGTG
CTAGGGGTTAGGCTTTTGAGCTTGAACGCCTGCGTGTGAACAGATGAAAAATCCTTCAGTACCCAAGTCCCAGTCTGTCCTATGGGGAGCAGTTTGGGGG
CGGCCGGCAGCAGGAGCCTGGGAAAGAGGCCCTCGCCAGGTGATGGCAGGGCCAGGGTGGCCTGGGGCACCCAGCGGAATGTGCTTAGTATTTGGTCACC
AGCCGTCATCCTGGGCTTTTCCTACTGTGTCTTGTTACAAGGCCTCAGCAATCCACAGAACTCTCTCTCCTTCCTTCCACCTGTCAGCTTCTCTGCTTCT
GAGATAAGAACCATTTGTGTAACACCAACACTTAACTTCAGAAAGACATGCATTATGTGGTGTAATCAAACCCGATGCTTTCAGATGACCTACTTACATC
TTCAATGTGGATAAGATAAAGAACAAAACACATGCATCTAAACTGCTGGGCAATCCAGTTGACTTTTAAATGTAAGAATGGAATTCCAAACACTTAACAC
ATTCAGCTATATGACAGAAAGTAAATCTATGGATATGGTATTTTGTGAATGATCTTTTAAATAAAAGAAAACCTTACGTAATATTT
- refseq_WBSCR1.F1 (forward primer)
- refseq_WBSCR1.R1 (reverse primer)
- Single exon skipping
Frame 0
DGKWRTSTPTTIGPTAASAAAEGPAAVLVAMVPVARRSCPQSPPTQHT*EIYLSIRFRAT*MLSLRI
SA*GVYG*SETKTQINLKDSAM*NSMKWIPLRK
P*HTMVHCWAIGHFVWTLQKAENKIKVALDSEK
VDQMTEASGMTS*GAGEVVAQATGEQAPPWAAAS
EMALPSVDPTWISENPQKRKEHRDHDSSLNLEQ
SRRPSIK*PIPTLLSSGVPGLERKSFKRSKNEP
AVGREWGVGG*SRTTAW*VPGQPSCSRHSCACHW
PPHSGNTACECMSAVNKWFLVHSGLCCIYLVPV
CAFFSFFRCFPIFLLSFFSCSLFSQQHMGFLGG
AEVAAVGGRLGCGAASFFLCFLFTLDTGPTPGVS
FHSLSASLLTCMLSSVLLGLPTKTRVTDRGNSR
DLVGIQL*WI*RIRGTLFSQLG*KYLQGPFHSY
LEGVLAP*PPPEWTVQADRLTRETVRLKGGAMEE
R*GVHGEDAVNACMHSHVCVSQLVHSFRRAWWR
LASLAGCSEWPAGFFSAGPRRFGGGGGGAGAAL
G*LRR*RSVSPHPCCLCVVWICS*YRQHNEILCF
PPPLQFRAKMGLESGTLLISFDKLYYAELSGTF
R*Q*RTAAV*VCGHILGTHWTVPMCRVQQLCGS
ARG*AFELERLRVNR*KILQYPSPSLSYGEQFGG
GRQQEPGKEALAR*WQGQGGLGHPAECA*YLVT
SRHPGLFLLCLVTRPQQSTELSLLPSTCQLLCF
*DKNHLCNTNT*LQKDMHYVV*SNPMLSDDLLTS
SMWIR*RTKHMHLNCWAIQLTFKCKNGIPNT*H
IQLYDRK*IYGYGIL*MIF*IKENLT*Y
Frame 1
TANGGLRHLRRSGLQQLRRRQRVPRQCWWPWFP*PEGVAHRAPLHSIRRKSTFQYGSGRHRCYL*G
SQHKECTASQRQRHR*I*RILLCRIR*SGFP*GS
LDIRWCTVGRSVTSCGHCRRQKTR*RWLWIQKR
WTR*QRLQG*LLRGQGR*SPRRPANRPPHGQPL
QRWPSPPWIQHGFQRTHRRGKSTETTTPA*TSNS
RDAPQSSSQSQLCYLRGCQA*RGSRSKGARMSL
RLGGNGAWGVRAGPQPGESPGSRPAAATPAPAI
GLLTAETQLVSACQLLTSGF*YILGFAVSI*CLF
VRFFLSSAASPFSFCPFSPAPCFPSSTWGSSEE
QRWPPWGGVWAAVLRHFSFAFSLL*TLAQLQAF
PFIPSVLLF*PAC*VLYCWGFQQKPESLTEGTAE
TLLVFSCDGYRESEAPCFHN*DKNICRVLSIPI
*RESWLHDPLPSGLSKQIGSHEKQ*G*KGGLWK
SGRESTEKMQ*MLACIHTCVCPS*FTPFAVRGGG
WPLWLGAVNGQRVSFLLGQGALGVEGVVLVLHW
ADCGADAAFPPIPVACVLCGSVPSIGNIMRYCA
SHLPFSSEPKWV*NLALYSFPLINCTMQSCQEPS
DSSRGLQLSRSAATSWGHTGLFPCAGFSSYVGV
LGVRLLSLNACV*TDEKSFSTQVPVCPMGSSLG
AAGSRSLGKRPSPGDGRARVAWGTQRNVLSIWSP
AVILGFSYCVLLQGLSNPQNSLSFLPPVSFSAS
EIRTICVTPTLNFRKTCIMWCNQTRCFQMTYLH
LQCG*DKEQNTCI*TAGQSS*LLNVRMEFQTLNT
FSYMTESKSMDMVFCE*SFK*KKTLRNI
Frame 2
RQMADFDTYDDRAYSSFGGGRGSRGSAGGHGSRSQKELPTEPPYTAYVGNLPFNTVQGDIDAIFKD
LSIRSVRLVRDKDTDKFKGFCYVEFDEVDSLKE
ALTYDGALLGDRSLRVDIAEGRKQDKGGFGFRKG
GPDDRGFRDDFLGGRGGSRPGDRRTGPPMGSRF
RDGPPLRGSNMDFREPTEEERAQRPRLQLKPRT
VATPLNQVANPNSAIFGGARPREEVVQKEQE*AC
GWEGMGRGGLEQDHSLVSPRAAVLQPPLLRLPL
ASSQRKHSL*VHVSC*QVVFSTFWALLYLSSAC
LCVFFFLPLLPHFPSVLFLLLLVFPAAHGVPRRS
RGGRRGGAFGLRCCVIFPLLSLYFRHWPNSRRF
LSFPQCFSSDLHVEFCIAGASNKNQSH*QREQQ
RPCWYSAVMDIENQRHLVFTTRIKISAGSFPFLF
RGSPGSMTPSRVDCPSR*AHTRNSEAERGGYGR
AVGSPRRRCSECLHAFTRVCVPASSLLSPCVVE
AGLSGWVQ*MASGFLFCWAKALWGWRGWCWCCTG
LTAALTQRFPPSLLPVCCVDLFLV*AT**DTVL
PTSPSVQSQNGSRIWHFTHFL**IVLCRAVRNL
QIAVEDCSCLGLRPHLGDTLDCSHVQGSAVMWEC
*GLGF*A*TPACEQMKNPSVPKSQSVLWGAVWG
RPAAGAWERGPRQVMAGPGWPGAPSGMCLVFGH
QPSSWAFPTVSCYKASAIHRTLSPSFHLSASLLL
R*EPFV*HQHLTSERHALCGVIKPDAFR*PTYI
FNVDKIKNKTHASKLLGNPVDF*M*EWNSKHLT
HSAI*QKVNLWIWYFVNDLLNKRKPYVIF
Sequence NM_022170
GACGGCAAATGGCGGACTTCGACACCTACGACGATCGGGCCTACAGCAGCTTCGGCGGCGGCAGAGGGTCCCGCGGCAGTGCTGGTGGCCATGGTTCCCGTAGCCAGAAGGAGTTGCCCACAGAGCCCCCCTACACAGCATACGTAGGAAATCTACCTTTCAATACGGTTCAGGGCGACATAGATGCTATCTTTAAGGAT
CTCAGCATAAGGAGTGTACGGCTAGTCAGAGACAAAGACACAGATAAATTTAAAGGATTCTGCTATGTAGAATTCGATGAAGTGGATTCCCTTAAGGAAG
CCTTGACATACGATGGTGCACTGTTGGGCGATCGGTCACTTCGTGTGGACATTGCAGAAGGCAGAAAACAAGATAAAGGTGGCTTTGGATTCAGAAAAGG
TGGACCAGATGACAGAGGAATGGGTAGCTCTCGAGAATCTAGAGGTGGATGGGATTCCCGGGATGACTTCAATTCTGGCTTCAGGGATGACTTCTTAGGG
GGCAGGGGAGGTAGTCGCCCAGGCGACCGGCGAACAGGCCCCCCCATGGGCAGCCGCTTCAGAGATGGCCCTCCCCTCCGTGGATCCAACATGGATTTCA
GAGAACCCACAGAAGAGGAAAGAGCACAGAGACCACGACTCCAGCTTAAACCTCGAACAGTCGCGACGCCCCTCAATCAAGTAGCCAATCCCAACTCTGC
TATCTTCGGGGGTGCCAGGCCTAGAGAGGAAGTCGTTCAAAAGGAGCAAGAATGAGCCTGCGGTTGGGAGGGAATGGGGCGTGGGGGGTTAGAGCAGGAC
CACAGCCTGGTGAGTCCCCGGGCAGCCGTCCTGCAGCCGCCACTCCTGCGCCTGCCATTGGCCTCCTCACAGCGGAAACACAGCTTGTGAGTGCATGTCA
GCTGTTAACAAGTGGTTTTTAGTACATTCTGGGCTTTGCTGTATCTATCTAGTGCCTGTTTGTGCGTTTTTTTCTTTCTTCCGCTGCTTCCCCATTTTCC
TTCTGTCCTTTTTCTCCTGCTCCTTGTTTTCCCAGCAGCACATGGGGTTCCTCGGAGGAGCAGAGGTGGCCGCCGTGGGGGGGCGTTTGGGCTGCGGTGC
TGCGTCATTTTTCCTTTGCTTTCTCTTTACTTTAGACACTGGCCCAACTCCAGGCGTTTCCTTTCATTCCCTCAGTGCTTCTCTTCTGACCTGCATGTTG
AGTTCTGTATTGCTGGGGCTTCCAACAAAAACCAGAGTCACTGACAGAGGGAACAGCAGAGACCTTGTTGGTATTCAGCTGTGATGGATATAGAGAATCA
GAGGCACCTTGTTTTCACAACTAGGATAAAAATATCTGCAGGGTCCTTTCCATTCCTATTTAGAGGGAGTCCTGGCTCCATGACCCCCTCCCGAGTGGAC
TGTCCAAGCAGATAGGCTCACACGAGAAACAGTGAGGCTGAAAGGGGGGGCTATGGAAGAGCGGTAGGGAGTCCACGGAGAAGATGCAGTGAATGCTTGC
ATGCATTCACACGTGTGTGTGTCCCAGCTAGTTCACTCCTTTCGCCGTGCGTGGTGGAGGCTGGCCTCTCTGGCTGGGTGCAGTGAATGGCCAGCGGGTT
TCTTTTCTGCTGGGCCAAGGCGCTTTGGGGGTGGAGGGGGTGGTGCTGGTGCTGCACTGGGCTGACTGCGGCGCTGACGCAGCGTTTCCCCCCATCCCTG
TTGCCTGTGTGTTGTGTGGATCTGTTCCTAGTATAGGCAACATAATGAGATACTGTGCTTCCCACCTCCCCTTCAGTTCAGAGCCAAAATGGGTCTAGAA
TCTGGCACTTTACTCATTTCCTTTGATAAATTGTACTATGCAGAGCTGTCAGGAACCTTCAGATAGCAGTAGAGGACTGCAGCTGTCTAGGTCTGCGGCC
ACATCTTGGGGACACACTGGACTGTTCCCATGTGCAGGGTTCAGCAGTTATGTGGGAGTGCTAGGGGTTAGGCTTTTGAGCTTGAACGCCTGCGTGTGAA
CAGATGAAAAATCCTTCAGTACCCAAGTCCCAGTCTGTCCTATGGGGAGCAGTTTGGGGGCGGCCGGCAGCAGGAGCCTGGGAAAGAGGCCCTCGCCAGG
TGATGGCAGGGCCAGGGTGGCCTGGGGCACCCAGCGGAATGTGCTTAGTATTTGGTCACCAGCCGTCATCCTGGGCTTTTCCTACTGTGTCTTGTTACAA
GGCCTCAGCAATCCACAGAACTCTCTCTCCTTCCTTCCACCTGTCAGCTTCTCTGCTTCTGAGATAAGAACCATTTGTGTAACACCAACACTTAACTTCA
GAAAGACATGCATTATGTGGTGTAATCAAACCCGATGCTTTCAGATGACCTACTTACATCTTCAATGTGGATAAGATAAAGAACAAAACACATGCATCTA
AACTGCTGGGCAATCCAGTTGACTTTTAAATGTAAGAATGGAATTCCAAACACTTAACACATTCAGCTATATGACAGAAAGTAAATCTATGGATATGGTA
TTTTGTGAATGATCTTTTAAATAAAAGAAAACCTTACGTAATATTT
- refseq_WBSCR1.F1 (forward primer)
- refseq_WBSCR1.R1 (reverse primer)
- Single exon skipping
Frame 0
DGKWRTSTPTTIGPTAASAAAEGPAAVLVAMVPVARRSCPQSPPTQHT*EIYLSIRFRAT*MLSLRI
SA*GVYG*SETKTQINLKDSAM*NSMKWIPLRK
P*HTMVHCWAIGHFVWTLQKAENKIKVALDSEK
VDQMTEEWVALENLEVDGIPGMTSILASGMTS*G
AGEVVAQATGEQAPPWAAASEMALPSVDPTWIS
ENPQKRKEHRDHDSSLNLEQSRRPSIK*PIPTL
LSSGVPGLERKSFKRSKNEPAVGREWGVGG*SRT
TAW*VPGQPSCSRHSCACHWPPHSGNTACECMS
AVNKWFLVHSGLCCIYLVPVCAFFSFFRCFPIF
LLSFFSCSLFSQQHMGFLGGAEVAAVGGRLGCGA
ASFFLCFLFTLDTGPTPGVSFHSLSASLLTCML
SSVLLGLPTKTRVTDRGNSRDLVGIQL*WI*RI
RGTLFSQLG*KYLQGPFHSYLEGVLAP*PPPEWT
VQADRLTRETVRLKGGAMEER*GVHGEDAVNAC
MHSHVCVSQLVHSFRRAWWRLASLAGCSEWPAG
FFSAGPRRFGGGGGGAGAALG*LRR*RSVSPHPC
CLCVVWICS*YRQHNEILCFPPPLQFRAKMGLE
SGTLLISFDKLYYAELSGTFR*Q*RTAAV*VCG
HILGTHWTVPMCRVQQLCGSARG*AFELERLRVN
R*KILQYPSPSLSYGEQFGGGRQQEPGKEALAR
*WQGQGGLGHPAECA*YLVTSRHPGLFLLCLVT
RPQQSTELSLLPSTCQLLCF*DKNHLCNTNT*LQ
KDMHYVV*SNPMLSDDLLTSSMWIR*RTKHMHL
NCWAIQLTFKCKNGIPNT*HIQLYDRK*IYGYG
IL*MIF*IKENLT*Y
Frame 1
TANGGLRHLRRSGLQQLRRRQRVPRQCWWPWFP*PEGVAHRAPLHSIRRKSTFQYGSGRHRCYL*G
SQHKECTASQRQRHR*I*RILLCRIR*SGFP*GS
LDIRWCTVGRSVTSCGHCRRQKTR*RWLWIQKR
WTR*QRNG*LSRI*RWMGFPG*LQFWLQG*LLR
GQGR*SPRRPANRPPHGQPLQRWPSPPWIQHGFQ
RTHRRGKSTETTTPA*TSNSRDAPQSSSQSQLC
YLRGCQA*RGSRSKGARMSLRLGGNGAWGVRAG
PQPGESPGSRPAAATPAPAIGLLTAETQLVSACQ
LLTSGF*YILGFAVSI*CLFVRFFLSSAASPFS
FCPFSPAPCFPSSTWGSSEEQRWPPWGGVWAAV
LRHFSFAFSLL*TLAQLQAFPFIPSVLLF*PAC*
VLYCWGFQQKPESLTEGTAETLLVFSCDGYRES
EAPCFHN*DKNICRVLSIPI*RESWLHDPLPSG
LSKQIGSHEKQ*G*KGGLWKSGRESTEKMQ*MLA
CIHTCVCPS*FTPFAVRGGGWPLWLGAVNGQRV
SFLLGQGALGVEGVVLVLHWADCGADAAFPPIP
VACVLCGSVPSIGNIMRYCASHLPFSSEPKWV*N
LALYSFPLINCTMQSCQEPSDSSRGLQLSRSAA
TSWGHTGLFPCAGFSSYVGVLGVRLLSLNACV*
TDEKSFSTQVPVCPMGSSLGAAGSRSLGKRPSPG
DGRARVAWGTQRNVLSIWSPAVILGFSYCVLLQ
GLSNPQNSLSFLPPVSFSASEIRTICVTPTLNF
RKTCIMWCNQTRCFQMTYLHLQCG*DKEQNTCI*
TAGQSS*LLNVRMEFQTLNTFSYMTESKSMDMV
FCE*SFK*KKTLRNI
Frame 2
RQMADFDTYDDRAYSSFGGGRGSRGSAGGHGSRSQKELPTEPPYTAYVGNLPFNTVQGDIDAIFKD
LSIRSVRLVRDKDTDKFKGFCYVEFDEVDSLKE
ALTYDGALLGDRSLRVDIAEGRKQDKGGFGFRKG
GPDDRGMGSSRESRGGWDSRDDFNSGFRDDFLG
GRGGSRPGDRRTGPPMGSRFRDGPPLRGSNMDF
REPTEEERAQRPRLQLKPRTVATPLNQVANPNSA
IFGGARPREEVVQKEQE*ACGWEGMGRGGLEQD
HSLVSPRAAVLQPPLLRLPLASSQRKHSL*VHV
SC*QVVFSTFWALLYLSSACLCVFFFLPLLPHFP
SVLFLLLLVFPAAHGVPRRSRGGRRGGAFGLRC
CVIFPLLSLYFRHWPNSRRFLSFPQCFSSDLHV
EFCIAGASNKNQSH*QREQQRPCWYSAVMDIENQ
RHLVFTTRIKISAGSFPFLFRGSPGSMTPSRVD
CPSR*AHTRNSEAERGGYGRAVGSPRRRCSECL
HAFTRVCVPASSLLSPCVVEAGLSGWVQ*MASGF
LFCWAKALWGWRGWCWCCTGLTAALTQRFPPSL
LPVCCVDLFLV*AT**DTVLPTSPSVQSQNGSR
IWHFTHFL**IVLCRAVRNLQIAVEDCSCLGLRP
HLGDTLDCSHVQGSAVMWEC*GLGF*A*TPACE
QMKNPSVPKSQSVLWGAVWGRPAAGAWERGPRQ
VMAGPGWPGAPSGMCLVFGHQPSSWAFPTVSCYK
ASAIHRTLSPSFHLSASLLLR*EPFV*HQHLTS
ERHALCGVIKPDAFR*PTYIFNVDKIKNKTHAS
KLLGNPVDF*M*EWNSKHLTHSAI*QKVNLWIWY
FVNDLLNKRKPYVIF
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