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Sequences

Sequence NM_001007593

ATCAGAGGAAGAGGAAGGGGCGGAGCTGCTTTGCGGCCGGCCGCGGAGCAGTCAGCCGACTACAGAGAAGGGTAATCGGGTGTCCCCGGCGCCGCCCGGG GCCCTGAGGGCTGGCTAGGGTCCAGGCCGGGGGGGACGGGACAGACGAACCAGCCCCGTGTAGGAAGCGCGACAATGCCCCGCTACGGAGCGTCACTCCG CCAGAGCTGCCCCAGGTCCGGCCGGGAGCAGGGACAAGACGGGACCGCCGGAGCCCCCGGACTCCTTTGGATGGGCCTGGTGCTGGCGCTGGCGCTGGCG CTGGCGCTGGCGCTGGCTCTGTCTGACTCTCGGGTTCTCTGGGCTCCGGCAGAGGCTCACCCTCTTTCTCCCCAAGGCCATCCTGCCAGGTTACATCGCA TAGTGCCCCGGCTCCGAGATGTCTTTGGGTGGGGGAACCTCACCTGCCCAATCTGCAAAGGTCTATTCACCGCCATCAACCTCGGGCTGAAGAAGGAACC CAATGTGGCTCGCGTGGGCTCCGTGGCCATCAAGCTGTGCAATCTGCTGAAGATAGCACCACCTGCCGTGTGCCAATCCATTGTCCACCTCTTTGAGGAT GACATGGTGGAGGTGTGGAGACGCTCAGTGCTGAGCCCATCTGAGGCCTGTGGCCTGCTCCTGGGCTCCACCTGTGGGCACTGGGACATTTTCTCATCTT GGAACATCTCTTTGCCTACTGTGCCGAAGCCGCCCCCCAAACCCCCTAGCCCCCCAGCCCCAGGTGCCCCTGTCAGCCGCATCCTCTTCCTCACTGACCT GCACTGGGATCATGACTACCTGGAGGGCACGGACCCTGACTGTGCAGACCCACTGTGCTGCCGCCGGGGTTCTGGCCTGCCGCCCGCATCCCGGCCAGGT GCCGGATACTGGGGCGAATACAGCAAGTGTGACCTGCCCCTGAGGACCCTGGAGAGCCTGTTGAGTGGGCTGGGCCCAGCCGGCCCTTTTGATATGGTGT ACTGGACAGGAGACATCCCCGCACATGATGTCTGGCACCAGACTCGTCAGGACCAACTGCGGGCCCTGACCACCGTCACAGCACTTGTGAGGAAGTTCCT GGGGCCAGTGCCAGTGTACCCTGCTGTGGGTAACCATGAAAGCACACCTGTCAATAGCTTCCCTCCCCCCTTCATTGAGGGCAACCACTCCTCCCGCTGG CTCTATGAAGCGATGGCCAAGGCTTGGGAGCCCTGGCTGCCTGCCGAAGCCCTGCGCACCCTCAGGTACTTATCGTCCGTGGAAACCCAGGAAGGGAAAA GAAAGGTGCATATAATTGGCCACATTCCCCCAGGGCACTGTCTGAAGAGCTGGAGCTGGAATTATTACCGAATTGTAGCCAGGTATGAGAACACCCTGGC TGCTCAGTTCTTTGGCCACACTCATGTGGATGAATTTGAGGTCTTCTATGATGAAGAGACTCTGAGCCGGCCGCTGGCTGTAGCCTTCCTGGCACCCAGT GCAACTACCTACATCGGCCTTAATCCTGGTTACCGTGTGTACCAAATAGATGGAAACTACTCCGGGAGCTCTCACGTGGTCCTGGACCATGAGACCTACA TCCTGAATCTGACCCAGGCAAACATACCGGGAGCCATACCGCACTGGCAGCTTCTCTACAGGGCTCGAGAAACCTATGGGCTGCCCAACACACTGCCTAC CGCCTGGCACAACCTGGTATATCGCATGCGGGGCGACATGCAACTTTTCCAGACCTTCTGGTTTCTCTACCATAAGGGCCACCCACCCTCGGAGCCCTGT GGCACGCCCTGCCGTCTGGCTACTCTTTGTGCCCAGCTCTCTGCCCGTGCTGACAGCCCTGCTCTGTGCCGCCACCTGATGCCAGATGGGAGCCTCCCAG AGGCCCAGAGCCTGTGGCCAAGGCCACTGTTTTGCTAGGGCCCCAGGGCCCACATTTGGGAAAGTTCTTGATGTAGGAAAGGGTGAAAAAGCCCAAATGC TGCTGTGGTTCAACCAGGCAAGATCATCCGGTGAAAGAACCAGTCCCTGGGCCCCAAGGATGCCGGGGAAACAGGACCTTCTCCTTTCCTGGAGCTGGTT TAGCTGGATATGGGAGGGGGTTTGGCTGCCTGTGCCCAGGAGCTAGACTGCCTTGAGGCTGCTGTCCTTTCACAGCCATGGAGTAGAGGCCTAAGTTGAC ACTGCCCTGGGCAGACAAGACAGGAGCTGTCGCCCCAGGCCTGTGCTGCCCAGCCAGGAACCCTGTACTGCTGCTGCGACCTGATGCTGCCAGTCTGTTA AAATAAAGATAAGAGACTTGGACTCCA

refseq_SMPD1.F2 (forward primer)
refseq_SMPD1.R2 (reverse primer)
Exon skipping and alternative donor or acceptor

Frame 0

IRGRGRGGAALRPAAEQSADYREG*SGVPGAAR GPEGWLGSRPGGTGQTNQPRVGSATMPRYGASLR QSCPRSGREQGQDGTAGAPGLLWMGLVLALALA LALALALSDSRVLWAPAEAHPLSPQGHPARLHR IVPRLRDVFGWGNLTCPICKGLFTAINLGLKKEP NVARVGSVAIKLCNLLKIAPPAVCQSIVHLFED DMVEVWRRSVLSPSEACGLLLGSTCGHWDIFSS WNISLPTVPKPPPKPPSPPAPGAPVSRILFLTDL HWDHDYLEGTDPDCADPLCCRRGSGLPPASRPG AGYWGEYSKCDLPLRTLESLLSGLGPAGPFDMV YWTGDIPAHDVWHQTRQDQLRALTTVTALVRKFL GPVPVYPAVGNHESTPVNSFPPPFIEGNHSSRW LYEAMAKAWEPWLPAEALRTLRYLSSVETQEGK RKVHIIGHIPPGHCLKSWSWNYYRIVARYENTLA AQFFGHTHVDEFEVFYDEETLSRPLAVAFLAPS ATTYIGLNPGYRVYQIDGNYSGSSHVVLDHETY ILNLTQANIPGAIPHWQLLYRARETYGLPNTLPT AWHNLVYRMRGDMQLFQTFWFLYHKGHPPSEPC GTPCRLATLCAQLSARADSPALCRHLMPDGSLP EAQSLWPRPLFC*GPRAHIWESS*CRKG*KSPNA AVVQPGKIIR*KNQSLGPKDAGETGPSPFLELV *LDMGGGLAACAQELDCLEAAVLSQPWSRGLS* HCPGQTRQELSPQACAAQPGTLYCCCDLMLPVC* NKDKRLGL

Frame 1

SEEEEGAELLCGRPRSSQPTTEKGNRVSPAPPG ALRAG*GPGRGGRDRRTSPV*EARQCPATERHS ARAAPGPAGSRDKTGPPEPPDSFGWAWCWRWRWR WRWRWLCLTLGFSGLRQRLTLFLPKAILPGYIA *CPGSEMSLGGGTSPAQSAKVYSPPSTSG*RRN PMWLAWAPWPSSCAIC*R*HHLPCANPLSTSLRM TWWRCGDAQC*AHLRPVACSWAPPVGTGTFSHL GTSLCLLCRSRPPNPLAPQPQVPLSAASSSSLT CTGIMTTWRARTLTVQTHCAAAGVLACRPHPGQV PDTGANTASVTCP*GPWRAC*VGWAQPALLIWC TGQETSPHMMSGTRLVRTNCGP*PPSQHL*GSS WGQCQCTLLWVTMKAHLSIASLPPSLRATTPPAG SMKRWPRLGSPGCLPKPCAPSGTYRPWKPRKGK ERCI*LATFPQGTV*RAGAGIITEL*PGMRTPW LLSSLATLMWMNLRSSMMKRL*AGRWL*PSWHPV QLPTSALILVTVCTK*METTPGALTWSWTMRPT S*I*PRQTYREPYRTGSFSTGLEKPMGCPTHCL PPGTTWYIACGATCNFSRPSGFSTIRATHPRSPV ARPAVWLLFVPSSLPVLTALLCAAT*CQMGASQ RPRACGQGHCFARAPGPTFGKVLDVGKGEKAQM LLWFNQARSSGERTSPWAPRMPGKQDLLLSWSWF SWIWEGVWLPVPRS*TALRLLSFHSHGVEA*VD TALGRQDRSCRPRPVLPSQEPCTAAAT*CCQSV KIKIRDLDS

Frame 2

QRKRKGRSCFAAGRGAVSRLQRRVIGCPRRRPG P*GLARVQAGGDGTDEPAPCRKRDNAPLRSVTP PELPQVRPGAGTRRDRRSPRTPLDGPGAGAGAG AGAGAGSV*LSGSLGSGRGSPSFSPRPSCQVTSH SAPAPRCLWVGEPHLPNLQRSIHRHQPRAEEGT QCGSRGLRGHQAVQSAEDSTTCRVPIHCPPL*G *HGGGVETLSAEPI*GLWPAPGLHLWALGHFLIL EHLFAYCAEAAPQTP*PPSPRCPCQPHPLPH*P ALGS*LPGGHGP*LCRPTVLPPGFWPAARIPAR CRILGRIQQV*PAPEDPGEPVEWAGPSRPF*YGV LDRRHPRT*CLAPDSSGPTAGPDHRHSTCEEVP GASASVPCCG*P*KHTCQ*LPSPLH*GQPLLPL AL*SDGQGLGALAACRSPAHPQVLIVRGNPGREK KGAYNWPHSPRALSEELELELLPNCSQV*EHPG CSVLWPHSCG*I*GLL**RDSEPAAGCSLPGTQ CNYLHRP*SWLPCVPNRWKLLRELSRGPGP*DLH PESDPGKHTGSHTALAASLQGSRNLWAAQHTAY RLAQPGISHAGRHATFPDLLVSLP*GPPTLGAL WHALPSGYSLCPALCPC*QPCSVPPPDARWEPPR GPEPVAKATVLLGPQGPHLGKFLM*ERVKKPKC CCGSTRQDHPVKEPVPGPQGCRGNRTFSFPGAG LAGYGRGFGCLCPGARLP*GCCPFTAME*RPKLT LPWADKTGAVAPGLCCPARNPVLLLRPDAASLL K*R*ETWTP

Sequence NM_000543

ATCAGAGGAAGAGGAAGGGGCGGAGCTGCTTTGCGGCCGGCCGCGGAGCAGTCAGCCGACTACAGAGAAGGGTAATCGGGTGTCCCCGGCGCCGCCCGGG GCCCTGAGGGCTGGCTAGGGTCCAGGCCGGGGGGGACGGGACAGACGAACCAGCCCCGTGTAGGAAGCGCGACAATGCCCCGCTACGGAGCGTCACTCCG CCAGAGCTGCCCCAGGTCCGGCCGGGAGCAGGGACAAGACGGGACCGCCGGAGCCCCCGGACTCCTTTGGATGGGCCTGGTGCTGGCGCTGGCGCTGGCG CTGGCGCTGGCGCTGGCTCTGTCTGACTCTCGGGTTCTCTGGGCTCCGGCAGAGGCTCACCCTCTTTCTCCCCAAGGCCATCCTGCCAGGTTACATCGCA TAGTGCCCCGGCTCCGAGATGTCTTTGGGTGGGGGAACCTCACCTGCCCAATCTGCAAAGGTCTATTCACCGCCATCAACCTCGGGCTGAAGAAGGAACC CAATGTGGCTCGCGTGGGCTCCGTGGCCATCAAGCTGTGCAATCTGCTGAAGATAGCACCACCTGCCGTGTGCCAATCCATTGTCCACCTCTTTGAGGAT GACATGGTGGAGGTGTGGAGACGCTCAGTGCTGAGCCCATCTGAGGCCTGTGGCCTGCTCCTGGGCTCCACCTGTGGGCACTGGGACATTTTCTCATCTT GGAACATCTCTTTGCCTACTGTGCCGAAGCCGCCCCCCAAACCCCCTAGCCCCCCAGCCCCAGGTGCCCCTGTCAGCCGCATCCTCTTCCTCACTGACCT GCACTGGGATCATGACTACCTGGAGGGCACGGACCCTGACTGTGCAGACCCACTGTGCTGCCGCCGGGGTTCTGGCCTGCCGCCCGCATCCCGGCCAGGT GCCGGATACTGGGGCGAATACAGCAAGTGTGACCTGCCCCTGAGGACCCTGGAGAGCCTGTTGAGTGGGCTGGGCCCAGCCGGCCCTTTTGATATGGTGT ACTGGACAGGAGACATCCCCGCACATGATGTCTGGCACCAGACTCGTCAGGACCAACTGCGGGCCCTGACCACCGTCACAGCACTTGTGAGGAAGTTCCT GGGGCCAGTGCCAGTGTACCCTGCTGTGGGTAACCATGAAAGCACACCTGTCAATAGCTTCCCTCCCCCCTTCATTGAGGGCAACCACTCCTCCCGCTGG CTCTATGAAGCGATGGCCAAGGCTTGGGAGCCCTGGCTGCCTGCCGAAGCCCTGCGCACCCTCAGAATTGGGGGGTTCTATGCTCTTTCCCCATACCCCG GTCTCCGCCTCATCTCTCTCAATATGAATTTTTGTTCCCGTGAGAACTTCTGGCTCTTGATCAACTCCACGGATCCCGCAGGACAGCTCCAGTGGCTGGT GGGGGAGCTTCAGGCTGCTGAGGATCGAGGAGACAAAGTGCATATAATTGGCCACATTCCCCCAGGGCACTGTCTGAAGAGCTGGAGCTGGAATTATTAC CGAATTGTAGCCAGGTATGAGAACACCCTGGCTGCTCAGTTCTTTGGCCACACTCATGTGGATGAATTTGAGGTCTTCTATGATGAAGAGACTCTGAGCC GGCCGCTGGCTGTAGCCTTCCTGGCACCCAGTGCAACTACCTACATCGGCCTTAATCCTGGTTACCGTGTGTACCAAATAGATGGAAACTACTCCGGGAG CTCTCACGTGGTCCTGGACCATGAGACCTACATCCTGAATCTGACCCAGGCAAACATACCGGGAGCCATACCGCACTGGCAGCTTCTCTACAGGGCTCGA GAAACCTATGGGCTGCCCAACACACTGCCTACCGCCTGGCACAACCTGGTATATCGCATGCGGGGCGACATGCAACTTTTCCAGACCTTCTGGTTTCTCT ACCATAAGGGCCACCCACCCTCGGAGCCCTGTGGCACGCCCTGCCGTCTGGCTACTCTTTGTGCCCAGCTCTCTGCCCGTGCTGACAGCCCTGCTCTGTG CCGCCACCTGATGCCAGATGGGAGCCTCCCAGAGGCCCAGAGCCTGTGGCCAAGGCCACTGTTTTGCTAGGGCCCCAGGGCCCACATTTGGGAAAGTTCT TGATGTAGGAAAGGGTGAAAAAGCCCAAATGCTGCTGTGGTTCAACCAGGCAAGATCATCCGGTGAAAGAACCAGTCCCTGGGCCCCAAGGATGCCGGGG AAACAGGACCTTCTCCTTTCCTGGAGCTGGTTTAGCTGGATATGGGAGGGGGTTTGGCTGCCTGTGCCCAGGAGCTAGACTGCCTTGAGGCTGCTGTCCT TTCACAGCCATGGAGTAGAGGCCTAAGTTGACACTGCCCTGGGCAGACAAGACAGGAGCTGTCGCCCCAGGCCTGTGCTGCCCAGCCAGGAACCCTGTAC TGCTGCTGCGACCTGATGCTGCCAGTCTGTTAAAATAAAGATAAGAGACTTGGACTCCA

refseq_SMPD1.F2 (forward primer)
refseq_SMPD1.R2 (reverse primer)
Exon skipping and alternative donor or acceptor

Frame 0

IRGRGRGGAALRPAAEQSADYREG*SGVPGAAR GPEGWLGSRPGGTGQTNQPRVGSATMPRYGASLR QSCPRSGREQGQDGTAGAPGLLWMGLVLALALA LALALALSDSRVLWAPAEAHPLSPQGHPARLHR IVPRLRDVFGWGNLTCPICKGLFTAINLGLKKEP NVARVGSVAIKLCNLLKIAPPAVCQSIVHLFED DMVEVWRRSVLSPSEACGLLLGSTCGHWDIFSS WNISLPTVPKPPPKPPSPPAPGAPVSRILFLTDL HWDHDYLEGTDPDCADPLCCRRGSGLPPASRPG AGYWGEYSKCDLPLRTLESLLSGLGPAGPFDMV YWTGDIPAHDVWHQTRQDQLRALTTVTALVRKFL GPVPVYPAVGNHESTPVNSFPPPFIEGNHSSRW LYEAMAKAWEPWLPAEALRTLRIGGFYALSPYP GLRLISLNMNFCSRENFWLLINSTDPAGQLQWLV GELQAAEDRGDKVHIIGHIPPGHCLKSWSWNYY RIVARYENTLAAQFFGHTHVDEFEVFYDEETLS RPLAVAFLAPSATTYIGLNPGYRVYQIDGNYSGS SHVVLDHETYILNLTQANIPGAIPHWQLLYRAR ETYGLPNTLPTAWHNLVYRMRGDMQLFQTFWFL YHKGHPPSEPCGTPCRLATLCAQLSARADSPALC RHLMPDGSLPEAQSLWPRPLFC*GPRAHIWESS *CRKG*KSPNAAVVQPGKIIR*KNQSLGPKDAG ETGPSPFLELV*LDMGGGLAACAQELDCLEAAVL SQPWSRGLS*HCPGQTRQELSPQACAAQPGTLY CCCDLMLPVC*NKDKRLGL

Frame 1

SEEEEGAELLCGRPRSSQPTTEKGNRVSPAPPG ALRAG*GPGRGGRDRRTSPV*EARQCPATERHS ARAAPGPAGSRDKTGPPEPPDSFGWAWCWRWRWR WRWRWLCLTLGFSGLRQRLTLFLPKAILPGYIA *CPGSEMSLGGGTSPAQSAKVYSPPSTSG*RRN PMWLAWAPWPSSCAIC*R*HHLPCANPLSTSLRM TWWRCGDAQC*AHLRPVACSWAPPVGTGTFSHL GTSLCLLCRSRPPNPLAPQPQVPLSAASSSSLT CTGIMTTWRARTLTVQTHCAAAGVLACRPHPGQV PDTGANTASVTCP*GPWRAC*VGWAQPALLIWC TGQETSPHMMSGTRLVRTNCGP*PPSQHL*GSS WGQCQCTLLWVTMKAHLSIASLPPSLRATTPPAG SMKRWPRLGSPGCLPKPCAPSELGGSMLFPHTP VSASSLSI*IFVPVRTSGS*STPRIPQDSSSGW WGSFRLLRIEETKCI*LATFPQGTV*RAGAGIIT EL*PGMRTPWLLSSLATLMWMNLRSSMMKRL*A GRWL*PSWHPVQLPTSALILVTVCTK*METTPG ALTWSWTMRPTS*I*PRQTYREPYRTGSFSTGLE KPMGCPTHCLPPGTTWYIACGATCNFSRPSGFS TIRATHPRSPVARPAVWLLFVPSSLPVLTALLC AAT*CQMGASQRPRACGQGHCFARAPGPTFGKVL DVGKGEKAQMLLWFNQARSSGERTSPWAPRMPG KQDLLLSWSWFSWIWEGVWLPVPRS*TALRLLS FHSHGVEA*VDTALGRQDRSCRPRPVLPSQEPCT AAAT*CCQSVKIKIRDLDS

Frame 2

QRKRKGRSCFAAGRGAVSRLQRRVIGCPRRRPG P*GLARVQAGGDGTDEPAPCRKRDNAPLRSVTP PELPQVRPGAGTRRDRRSPRTPLDGPGAGAGAG AGAGAGSV*LSGSLGSGRGSPSFSPRPSCQVTSH SAPAPRCLWVGEPHLPNLQRSIHRHQPRAEEGT QCGSRGLRGHQAVQSAEDSTTCRVPIHCPPL*G *HGGGVETLSAEPI*GLWPAPGLHLWALGHFLIL EHLFAYCAEAAPQTP*PPSPRCPCQPHPLPH*P ALGS*LPGGHGP*LCRPTVLPPGFWPAARIPAR CRILGRIQQV*PAPEDPGEPVEWAGPSRPF*YGV LDRRHPRT*CLAPDSSGPTAGPDHRHSTCEEVP GASASVPCCG*P*KHTCQ*LPSPLH*GQPLLPL AL*SDGQGLGALAACRSPAHPQNWGVLCSFPIPR SPPHLSQYEFLFP*ELLALDQLHGSRRTAPVAG GGASGC*GSRRQSAYNWPHSPRALSEELELELL PNCSQV*EHPGCSVLWPHSCG*I*GLL**RDSEP AAGCSLPGTQCNYLHRP*SWLPCVPNRWKLLRE LSRGPGP*DLHPESDPGKHTGSHTALAASLQGS RNLWAAQHTAYRLAQPGISHAGRHATFPDLLVSL P*GPPTLGALWHALPSGYSLCPALCPC*QPCSV PPPDARWEPPRGPEPVAKATVLLGPQGPHLGKF LM*ERVKKPKCCCGSTRQDHPVKEPVPGPQGCRG NRTFSFPGAGLAGYGRGFGCLCPGARLP*GCCP FTAME*RPKLTLPWADKTGAVAPGLCCPARNPV LLLRPDAASLLK*R*ETWTP