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Sequence NM_001025778
GATGAGCAATGAGGGAATAATCCCCATTCCGTCTGGAATCAGAGGGCGGGCCTGCCGCGGAGGCATCTGGGAAACGGAGTTCCCAGGCAGGCGAAGCCCAGGGGCCGTCAGGAAGTCAGCGGCGCCTGCGCGGGCGCCTCAGATTCAGGATGCGCGCGCAGCGCTCTCCGCCCAGCGGAAGTTTTCGCTGGGCAACTGAG
AAGGTGAGTCCGGGTAACTGCGGAGGATCCGAGGGTCAGGCTGCAGAAGCCCAGAATCCCACCCCAGTCCCCAAGTACAGAGGTCGCTGTCAAGATGGAG
TTTCCAACCCAGTAAATCCAAGGGCCAGACCGTGACCTCATAAAGCATGATCTCCTTCTGTCCAGACTGTGGCAAAAGTATCCAAGCGGCATTCAAATTC
TGCCCCTACTGTGGAAATTCTTTGCCTGTAGAGGAGCATGTAGGGTCCCAGACCTTTGTCAATCCACATGTGTCATCCTTCCAAGGCTCCGGGAGCAGAC
CCCCAACCCCCAAAAGCAGCCCTCAGAAGACCAGGAAGAGCCCTCAGGTGACCAGGGGTAGCCCTCAGAAGACCAGCTGTAGCCCTCAGAAGACCAGGCA
GAGCCCTCAGACGCTGAAGCGGAGCCGAGTGACCACCTCACTTGAAGCTTTGCCCACAGGGACAGTGCTGACAGACAAGAGTGGGCGACAGTGGAAGCTG
AAGTCCTTCCAGACCAGGGACAACCAGGGCATTCTCTATGAAGCTGCACCCACCTCCACCCTCACCTGTGACTCAGGACCACAGAAGCAAAAGTTCTCAC
TCAAACTGGATGCCAAGGATGGGCGCTTGTTCAATGAGCAGAACTTCTTCCAGCGGGCCGCCAAGCCTCTGCAAGTCAACAAGTGGAAGAAGCTGTACTC
GACCCCACTGCTGGCCATCCCTACCTGCATGGGTTTCGGTGTTCACCAGGACAAATACAGGTTCTTGGTGTTACCCAGCCTGGGGAGGAGCCTTCAGTCG
GCCCTGGATGTCAGCCCAAAGCATGTGCTGTCAGAGAGGTCTGTGCTGCAGGTGGCCTGCCGGCTGCTGGATGCCCTGGAGTTCCTCCATGAGAATGAGT
ATGTTCATGGAAATGTGACAGCTGAAAATATCTTTGTGGATCCAGAGGACCAGAGTCAGGTGACTTTGGCAGGCTATGGCTTCGCCTTCCGCTATTGCCC
AAGTGGCAAACACGTGGCCTACGTGGAAGGCAGCAGGAGCCCTCACGAGGGGGACCTTGAGTTCATTAGCATGGACCTGCACAAGGGATGCGGGCCCTCC
CGCCGCAGCGACCTCCAGAGCCTGGGCTACTGCATGCTGAAGTGGCTCTACGGGTTTCTGCCATGGACAAATTGCCTTCCCAACACTGAGGACATCATGA
AGCAAAAACAGAAGTTTGTTGATAAGCCGGGGCCCTTCGTGGGACCCTGCGGTCACTGGATCAGGCCCTCAGAGACCCTGCAGAAGTACCTGAAGGTGGT
GATGGCCCTCACGTATGAGGAGAAGCCGCCCTACGCCATGCTGAGGAACAACCTAGAAGCTTTGCTGCAGGATCTGCGTGTGTCTCCATATGACCCCATT
GGCCTCCCGATGGTGCCCTAGGTGGAATCCAGAACTTTCCATTTGCAGTGTGCAACAGAAAAAAAAAAATGAAGTAATGTGACTCAAGGCCTGCTGTTTA
ATCACAGATAAGCTTCTAGAACAAGCCCTGGAATGTGCATTCCTGCCACTGGTTTCAGGATACTCATCAGTCCTGATTAGCCTCCCGGAGGGCCCCAGTT
TCCCTCCCGTGAATGTGAAGTTCCCCATCTTGGTGGCCTGCCCTTCAGCCAGTGTCCTAGCAAAGCTGGATGGGGTTGGGCCGGCCCACAGGGGGGACCC
CTCCTACCCTTGACACCTCTGTGCTTTGGTAATAAATTGTTTTACCAGAG
- refseq_VRK3.F2 (forward primer)
- refseq_VRK3.R2 (reverse primer)
- Single exon skipping
Frame 0
DEQ*GNNPHSVWNQRAGLPRRHLGNGVPRQAKPRGRQEVSGACAGASDSGCARSALRPAEVFAGQLR
R*VRVTAEDPRVRLQKPRIPPQSPSTEVAVKME
FPTQ*IQGPDRDLIKHDLLLSRLWQKYPSGIQI
LPLLWKFFACRGACRVPDLCQSTCVILPRLREQT
PNPQKQPSEDQEEPSGDQG*PSEDQL*PSEDQA
EPSDAEAEPSDHLT*SFAHRDSADRQEWATVEA
EVLPDQGQPGHSL*SCTHLHPHL*LRTTEAKVLT
QTGCQGWALVQ*AELLPAGRQASASQQVEEAVL
DPTAGHPYLHGFRCSPGQIQVLGVTQPGEEPSV
GPGCQPKACAVREVCAAGGLPAAGCPGVPP*E*V
CSWKCDS*KYLCGSRGPESGDFGRLWLRLPLLP
KWQTRGLRGRQQEPSRGGP*VH*HGPAQGMRAL
PPQRPPEPGLLHAEVALRVSAMDKLPSQH*GHHE
AKTEVC**AGALRGTLRSLDQALRDPAEVPEGG
DGPHV*GEAALRHAEEQPRSFAAGSACVSI*PH
WPPDGALGGIQNFPFAVCNRKKKMK*CDSRPAV*
SQISF*NKPWNVHSCHWFQDTHQS*LASRRAPV
SLP*M*SSPSWWPALQPVS*QSWMGLGRPTGGT
PPTLDTSVLW**IVLPE
Frame 1
MSNEGIIPIPSGIRGRACRGGIWETEFPGRRSPGAVRKSAAPARAPQIQDARAALSAQRKFSLGN*
EGESG*LRRIRGSGCRSPESHPSPQVQRSLSRWS
FQPSKSKGQTVTS*SMISFCPDCGKSIQAAFKF
CPYCGNSLPVEEHVGSQTFVNPHVSSFQGSGSR
PPTPKSSPQKTRKSPQVTRGSPQKTSCSPQKTRQ
SPQTLKRSRVTTSLEALPTGTVLTDKSGRQWKL
KSFQTRDNQGILYEAAPTSTLTCDSGPQKQKFS
LKLDAKDGRLFNEQNFFQRAAKPLQVNKWKKLYS
TPLLAIPTCMGFGVHQDKYRFLVLPSLGRSLQS
ALDVSPKHVLSERSVLQVACRLLDALEFLHENE
YVHGNVTAENIFVDPEDQSQVTLAGYGFAFRYCP
SGKHVAYVEGSRSPHEGDLEFISMDLHKGCGPS
RRSDLQSLGYCMLKWLYGFLPWTNCLPNTEDIM
KQKQKFVDKPGPFVGPCGHWIRPSETLQKYLKVV
MALTYEEKPPYAMLRNNLEALLQDLRVSPYDPI
GLPMVP*VESRTFHLQCATEKKK*SNVTQGLLF
NHR*ASRTSPGMCIPATGFRILISPD*PPGGPQF
PSRECEVPHLGGLPFSQCPSKAGWGWAGPQGGP
LLPLTPLCFGNKLFYQ
Frame 2
*AMRE*SPFRLESEGGPAAEASGKRSSQAGEAQGPSGSQRRLRGRLRFRMRAQRSPPSGSFRWATE
KVSPGNCGGSEGQAAEAQNPTPVPKYRGRCQDG
VSNPVNPRARP*PHKA*SPSVQTVAKVSKRHSNS
APTVEILCL*RSM*GPRPLSIHMCHPSKAPGAD
PQPPKAALRRPGRALR*PGVALRRPAVALRRPG
RALRR*SGAE*PPHLKLCPQGQC*QTRVGDSGS*
SPSRPGTTRAFSMKLHPPPPSPVTQDHRSKSSH
SNWMPRMGACSMSRTSSSGPPSLCKSTSGRSCT
RPHCWPSLPAWVSVFTRTNTGSWCYPAWGGAFSR
PWMSAQSMCCQRGLCCRWPAGCWMPWSSSMRMS
MFMEM*QLKISLWIQRTRVR*LWQAMASPSAIA
QVANTWPTWKAAGALTRGTLSSLAWTCTRDAGPP
AAATSRAWATAC*SGSTGFCHGQIAFPTLRTS*
SKNRSLLISRGPSWDPAVTGSGPQRPCRST*RW
*WPSRMRRSRPTPC*GTT*KLCCRICVCLHMTPL
ASRWCPRWNPELSICSVQQKKKNEVM*LKACCL
ITDKLLEQALECAFLPLVSGYSSVLISLPEGPS
FPPVNVKFPILVACPSASVLAKLDGVGPAHRGDP
SYP*HLCALVINCFTR
Sequence NM_016440
GATGAGCAATGAGGGAATAATCCCCATTCCGTCTGGAATCAGAGGGCGGGCCTGCCGCGGAGGCATCTGGGAAACGGAGTTCCCAGGCAGGCGAAGCCCAGGGGCCGTCAGGAAGTCAGCGGCGCCTGCGCGGGCGCCTCAGATTCAGGATGCGCGCGCAGCGCTCTCCGCCCAGCGGAAGTTTTCGCTGGGCAACTGAG
AAGGTGAGTCCGGGTAACTGCGGAGGATCCGAGGGTCAGGCTGCAGAAGCCCAGAATCCCACCCCAGTCCCCAAGTACAGAGGTCGCTGTCAAGATGGAG
TTTCCAACCCAGTAAATCCAAGGGCCAGACCGTGACCTCATAAAGCATGATCTCCTTCTGTCCAGACTGTGGCAAAAGTATCCAAGCGGCATTCAAATTC
TGCCCCTACTGTGGAAATTCTTTGCCTGTAGAGGAGCATGTAGGGTCCCAGACCTTTGTCAATCCACATGTGTCATCCTTCCAAGGCTCAAAGAGAGGGC
TGAACTCCAGTTTTGAAACCTCTCCTAAGAAAGTGAAATGGTCCAGCACCGTCACCTCTCCCCGATTATCCCTCTTCTCAGATGGTGACAGTTCTGAGTC
TGAAGATACTCTGAGTTCCTCTGAGAGATCCAAAGGCTCCGGGAGCAGACCCCCAACCCCCAAAAGCAGCCCTCAGAAGACCAGGAAGAGCCCTCAGGTG
ACCAGGGGTAGCCCTCAGAAGACCAGCTGTAGCCCTCAGAAGACCAGGCAGAGCCCTCAGACGCTGAAGCGGAGCCGAGTGACCACCTCACTTGAAGCTT
TGCCCACAGGGACAGTGCTGACAGACAAGAGTGGGCGACAGTGGAAGCTGAAGTCCTTCCAGACCAGGGACAACCAGGGCATTCTCTATGAAGCTGCACC
CACCTCCACCCTCACCTGTGACTCAGGACCACAGAAGCAAAAGTTCTCACTCAAACTGGATGCCAAGGATGGGCGCTTGTTCAATGAGCAGAACTTCTTC
CAGCGGGCCGCCAAGCCTCTGCAAGTCAACAAGTGGAAGAAGCTGTACTCGACCCCACTGCTGGCCATCCCTACCTGCATGGGTTTCGGTGTTCACCAGG
ACAAATACAGGTTCTTGGTGTTACCCAGCCTGGGGAGGAGCCTTCAGTCGGCCCTGGATGTCAGCCCAAAGCATGTGCTGTCAGAGAGGTCTGTGCTGCA
GGTGGCCTGCCGGCTGCTGGATGCCCTGGAGTTCCTCCATGAGAATGAGTATGTTCATGGAAATGTGACAGCTGAAAATATCTTTGTGGATCCAGAGGAC
CAGAGTCAGGTGACTTTGGCAGGCTATGGCTTCGCCTTCCGCTATTGCCCAAGTGGCAAACACGTGGCCTACGTGGAAGGCAGCAGGAGCCCTCACGAGG
GGGACCTTGAGTTCATTAGCATGGACCTGCACAAGGGATGCGGGCCCTCCCGCCGCAGCGACCTCCAGAGCCTGGGCTACTGCATGCTGAAGTGGCTCTA
CGGGTTTCTGCCATGGACAAATTGCCTTCCCAACACTGAGGACATCATGAAGCAAAAACAGAAGTTTGTTGATAAGCCGGGGCCCTTCGTGGGACCCTGC
GGTCACTGGATCAGGCCCTCAGAGACCCTGCAGAAGTACCTGAAGGTGGTGATGGCCCTCACGTATGAGGAGAAGCCGCCCTACGCCATGCTGAGGAACA
ACCTAGAAGCTTTGCTGCAGGATCTGCGTGTGTCTCCATATGACCCCATTGGCCTCCCGATGGTGCCCTAGGTGGAATCCAGAACTTTCCATTTGCAGTG
TGCAACAGAAAAAAAAAAATGAAGTAATGTGACTCAAGGCCTGCTGTTTAATCACAGATAAGCTTCTAGAACAAGCCCTGGAATGTGCATTCCTGCCACT
GGTTTCAGGATACTCATCAGTCCTGATTAGCCTCCCGGAGGGCCCCAGTTTCCCTCCCGTGAATGTGAAGTTCCCCATCTTGGTGGCCTGCCCTTCAGCC
AGTGTCCTAGCAAAGCTGGATGGGGTTGGGCCGGCCCACAGGGGGGACCCCTCCTACCCTTGACACCTCTGTGCTTTGGTAATAAATTGTTTTACCAGAG
- refseq_VRK3.F2 (forward primer)
- refseq_VRK3.R2 (reverse primer)
- Single exon skipping
Frame 0
DEQ*GNNPHSVWNQRAGLPRRHLGNGVPRQAKPRGRQEVSGACAGASDSGCARSALRPAEVFAGQLR
R*VRVTAEDPRVRLQKPRIPPQSPSTEVAVKME
FPTQ*IQGPDRDLIKHDLLLSRLWQKYPSGIQI
LPLLWKFFACRGACRVPDLCQSTCVILPRLKERA
ELQF*NLS*ESEMVQHRHLSPIIPLLRW*QF*V
*RYSEFL*EIQRLREQTPNPQKQPSEDQEEPSG
DQG*PSEDQL*PSEDQAEPSDAEAEPSDHLT*SF
AHRDSADRQEWATVEAEVLPDQGQPGHSL*SCT
HLHPHL*LRTTEAKVLTQTGCQGWALVQ*AELL
PAGRQASASQQVEEAVLDPTAGHPYLHGFRCSPG
QIQVLGVTQPGEEPSVGPGCQPKACAVREVCAA
GGLPAAGCPGVPP*E*VCSWKCDS*KYLCGSRG
PESGDFGRLWLRLPLLPKWQTRGLRGRQQEPSRG
GP*VH*HGPAQGMRALPPQRPPEPGLLHAEVAL
RVSAMDKLPSQH*GHHEAKTEVC**AGALRGTL
RSLDQALRDPAEVPEGGDGPHV*GEAALRHAEEQ
PRSFAAGSACVSI*PHWPPDGALGGIQNFPFAV
CNRKKKMK*CDSRPAV*SQISF*NKPWNVHSCH
WFQDTHQS*LASRRAPVSLP*M*SSPSWWPALQP
VS*QSWMGLGRPTGGTPPTLDTSVLW**IVLPE
Frame 1
MSNEGIIPIPSGIRGRACRGGIWETEFPGRRSPGAVRKSAAPARAPQIQDARAALSAQRKFSLGN*
EGESG*LRRIRGSGCRSPESHPSPQVQRSLSRWS
FQPSKSKGQTVTS*SMISFCPDCGKSIQAAFKF
CPYCGNSLPVEEHVGSQTFVNPHVSSFQGSKRG
LNSSFETSPKKVKWSSTVTSPRLSLFSDGDSSES
EDTLSSSERSKGSGSRPPTPKSSPQKTRKSPQV
TRGSPQKTSCSPQKTRQSPQTLKRSRVTTSLEA
LPTGTVLTDKSGRQWKLKSFQTRDNQGILYEAAP
TSTLTCDSGPQKQKFSLKLDAKDGRLFNEQNFF
QRAAKPLQVNKWKKLYSTPLLAIPTCMGFGVHQ
DKYRFLVLPSLGRSLQSALDVSPKHVLSERSVLQ
VACRLLDALEFLHENEYVHGNVTAENIFVDPED
QSQVTLAGYGFAFRYCPSGKHVAYVEGSRSPHE
GDLEFISMDLHKGCGPSRRSDLQSLGYCMLKWLY
GFLPWTNCLPNTEDIMKQKQKFVDKPGPFVGPC
GHWIRPSETLQKYLKVVMALTYEEKPPYAMLRN
NLEALLQDLRVSPYDPIGLPMVP*VESRTFHLQC
ATEKKK*SNVTQGLLFNHR*ASRTSPGMCIPAT
GFRILISPD*PPGGPQFPSRECEVPHLGGLPFS
QCPSKAGWGWAGPQGGPLLPLTPLCFGNKLFYQ
Frame 2
*AMRE*SPFRLESEGGPAAEASGKRSSQAGEAQGPSGSQRRLRGRLRFRMRAQRSPPSGSFRWATE
KVSPGNCGGSEGQAAEAQNPTPVPKYRGRCQDG
VSNPVNPRARP*PHKA*SPSVQTVAKVSKRHSNS
APTVEILCL*RSM*GPRPLSIHMCHPSKAQREG
*TPVLKPLLRK*NGPAPSPLPDYPSSQMVTVLS
LKIL*VPLRDPKAPGADPQPPKAALRRPGRALR*
PGVALRRPAVALRRPGRALRR*SGAE*PPHLKL
CPQGQC*QTRVGDSGS*SPSRPGTTRAFSMKLH
PPPPSPVTQDHRSKSSHSNWMPRMGACSMSRTSS
SGPPSLCKSTSGRSCTRPHCWPSLPAWVSVFTR
TNTGSWCYPAWGGAFSRPWMSAQSMCCQRGLCC
RWPAGCWMPWSSSMRMSMFMEM*QLKISLWIQRT
RVR*LWQAMASPSAIAQVANTWPTWKAAGALTR
GTLSSLAWTCTRDAGPPAAATSRAWATAC*SGS
TGFCHGQIAFPTLRTS*SKNRSLLISRGPSWDPA
VTGSGPQRPCRST*RW*WPSRMRRSRPTPC*GT
T*KLCCRICVCLHMTPLASRWCPRWNPELSICS
VQQKKKNEVM*LKACCLITDKLLEQALECAFLPL
VSGYSSVLISLPEGPSFPPVNVKFPILVACPSA
SVLAKLDGVGPAHRGDPSYP*HLCALVINCFTR
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