Sequences - Rnomics 📊 Palace

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Sequences

Sequence NM_206860

CCGGGGGGTTTAAGAGAAGAGCCTTTCGGACCACACCGGCGCTCACGCTCATACCCGCACGCCCCGGGCAGAGCCGCGCACGCCGGCCACACTCGGGCGC GCGCCGGCCACACTCGCGCGCACACATACGCGGCGCTCGCCCCCCGGCCCCCGGCTCGGGCCGCGAGTCGCAGCTCCCTGCCGCCGCTCCCGCCGCCACG GATGCCCGCAGCTGCTCCCCTCTGCAGTGCAGCAACCCCGGCCGCCGGCCGGCTCGCCCCGGCTCCCGGCTGCAGGAATCGCGCCAGGACGCTGGCCCCG CTCGCGGCTAGCTTGCACGCCAGGGCACAGCGAGGATGGGAGGGTCGCAGTCCCTGCAGCCAGCCCCAGCCAGCGACCTGAACCTGGAGGCTTCCGAGGC AATGAGTTCCGATTCTGAAGAGGCATTTGAGACCCCGGAGTCAACGACCCCTGTCAAAGCTCCGCCAGCTCCACCCCCACCACCCCCCGAAGTCATCCCA GAACCCGAGGTCAGCACACAGCCACCCCCGGAAGAACCAGGATGTGGTTCTGAGACAGTCCCTGTCCCTGATGGCCCACGGAGCGACTCGGTGGAAGGAA GTCCCTTCCGTCCCCCGTCACACTCCTTCTCTGCCGTCTTCGATGAAGACAAGCCGATAGCCAGCAGTGGGACTTACAACTTGGACTTTGACAACATTGA GCTTGTGGATACCTTTCAGACCTTGGAGCCTCGTGCCTCAGACGCTAAGAATCAGGAGGGCAAAGTGAACACACGGAGGAAGTCCACGGATTCCGTCCCC ATCTCTAAGTCTACACTGTCCCGGTCGCTCAGCCTGCAAGCCAGTGACTTTGATGGTGCTTCTTCCTCAGGCAATCCCGAGGCCGTGGCCCTTGCCCCAG ATGCATATAGCACGGGTTCCAGCAGTGCTTCTAGTACCCTTAAGCGAACTAAAAAACCGAGGCCGCCTTCCTTAAAAAAGAAACAGACCACCAAGAAACC CACAGAGACCCCCCCAGTGAAGGAGACGCAACAGGAGCCAGATGAAGAGAGCCTTGTCCCCAGTGGGGAGAATCTAGCATCTGAGACGAAAACGGAATCT GCCAAGACGGAAGGTCCTAGCCCAGCCTTATTGGAGGAGACGCCCCTTGAGCCCGCTGTGGGGCCCAAAGCTGCCTGCCCTCTGGACTCAGAGAGTGCAG AAGGGGTTGTCCCCCCGGCTTCTGGAGGTGGCAGAGTGCAGAACTCACCCCCTGTCGGGAGGAAAACGCTGCCTCTTACCACGGCCCCGGAGGCAGGGGA GGTAACCCCATCGGATAGCGGGGGGCAAGAGGACTCTCCAGCCAAAGGGCTCTCCGTAAGGCTGGAGTTTGACTATTCTGAGGACAAGAGTAGTTGGGAC AACCAGCAGGAAAACCCCCCTCCTACCAAAAAGATAGGCAAAAAGCCAGTTGCCAAAATGCCCCTGAGGAGGCCAAAGATGAAAAAGACACCCGAGAAAC TTGACAACACTCCTGCCTCACCTCCCAGATCCCCTGCTGAACCCAATGACATCCCCATTGCTAAAGGTACTTACACCTTTGATATTGACAAGTGGGATGA CCCCAATTTTAACCCTTTTTCTTCCACCTCAAAAATGCAGGAGTCTCCCAAACTGCCCCAACAATCATACAACTTTGACCCAGACACCTGTGATGAGTCC GTTGACCCCTTTAAGACATCCTCTAAGACCCCCAGCTCACCTTCTAAATCCCCAGCCTCCTTTGAGATCCCAGCCAGTGCTATGGAAGCCAATGGAGTGG ACGGGGATGGGCTAAACAAGCCCGCCAAGAAGAAGAAGACGCCCCTAAAGACTGACACATTTAGGGTGAAAAAGTCGCCAAAACGGTCTCCTCTCTCTGA TCCACCTTCCCAGGACCCCACCCCAGCTGCTACACCAGAAACACCACCAGTGATCTCTGCGGTGGTCCACGCCACAGATGAGGAAAAGCTGGCGGTCACC AACCAGAAGTGGACGTGCATGACAGTGGACCTAGAGGCTGACAAACAGGACTACCCGCAGCCCTCGGACCTGTCCACCTTTGTAAACGAGACCAAATTCA GTTCACCCACTGAGGAGTTGGATTACAGAAACTCCTATGAAATTGAATATATGGAGAAAATTGGCTCCTCCTTACCTCAGGACGACGATGCCCCGAAGAA GCAGGCCTTGTACCTTATGTTTGACACTTCTCAGGAGAGCCCTGTCAAGTCATCTCCCGTCCGCATGTCAGAGTCCCCGACGCCGTGTTCAGGGTCAAGT TTTGAAGAGACTGAAGCCCTTGTGAACACTGCTGCGAAAAACCAGCATCCTGTCCCACGAGGACTGGCCCCTAACCAAGAGTCACACTTGCAGGTGCCAG AGAAATCCTCCCAGAAGGAGCTGGAGGCCATGGGCTTGGGCACCCCTTCAGAAGCGATTGAAATTACAGCTCCCGAGGGCTCCTTTGCCTCTGCTGACGC CCTCCTCAGCAGGCTAGCTCACCCCGTCTCTCTCTGTGGTGCACTTGACTATCTGGAGCCCGACTTAGCAGAAAAGAACCCCCCACTATTCGCTCAGAAA CTCCAGAGAGAGGCTGCTCACCCAACAGACGTCTCCATCTCCAAAACAGCCTTGTACTCCCGCATCGGGACCGCTGAGGTGGAGAAACCTGCAGGCCTTC TGTTCCAGCAGCCCGACCTGGACTCTGCCCTCCAGATCGCCAGAGCAGAGATCATAACCAAGGAGAGAGAGGTCTCAGAATGGAAAGATAAATATGAAGA AAGCAGGCGGGAAGTGATGGAAATGAGGAAAATAGTGGCCGAGTATGAGAAGACCATCGCTCAGATGATAGAGGACGAACAGAGAGAGAAGTCAGTCTCC CACCAGACGGTGCAGCAGCTGGTTCTGGAGAAGGAGCAAGCCCTGGCCGACCTGAACTCCGTGGAGAAGTCTCTGGCCGACCTCTTCAGAAGATATGAGA AGATGAAGGAGGTCCTAGAAGGCTTCCGCAAGAATGAAGAGGTGTTGAAGAGATGTGCGCAGGAGTACCTGTCCCGGGTGAAGAAGGAGGAGCAGAGGTA CCAGGCCCTGAAGGTGCACGCGGAGGAGAAACTGGACAGGGCCAATGCTGAGATTGCTCAGGTTCGAGGCAAGGCCCAGCAGGAGCAAGCCGCCCACCAG GCCAGCCTGCGGAAGGAGCAGCTGCGAGTGGACGCCCTGGAAAGGACGCTGGAGCAGAAGAATAAAGAAATAGAAGAACTCACCAAGATTTGTGACGAAC TGATTGCCAAAATGGGGAAAAGCTAACTCTGAACCGAATGTTTTGGACTTAACTGTTGCGTGCAATATGACCGTCGGCACACTGCTGTTCCTCCAGTTCC ATGGACAGGTTCTGTTTTCACTTTTTCGTATGCACTACTGTATTTCCTTTCTAAATAAAATTGATTTGATTGTATGCAGTACTAAGGAGACTATCAGAAT TTCTTGCTATTGGTTTGCATTTTCCTAGTATAATTCATAGCAAGTTGACCTCAGAGTTCCTGTATCAGGGAGATTGTCTGATTCTCTAATAAAAGACACA TTGCTGACCTTGGCCTTGCCCTTTGTACACAAGTTCCCAGGGTGAGCAGCTTTTGGATTTAATATGAACATGTACAGCGTGCATAGGGACTCTTGCCTTA AGGAGTGTAAACTTGATCTGCATTTGCTGATTTGTTTTTAAAAAAACAAGAAATGCATGTTTCAAATAAAATTCTCTATTGTAAATAAAATTTTTTCTTT GGATCTTGGCAA

refseq_TACC2.F1 (forward primer)
refseq_TACC2.R1 (reverse primer)
Single exon skipping

Frame 0

PGGLREEPFGPHRRSRSYPHAPGRAAHAGHTRA RAGHTRAHTYAALAPRPPARAASRSSLPPLPPPR MPAAAPLCSAATPAAGRLAPAPGCRNRARTLAP LAASLHARAQRGWEGRSPCSQPQPAT*TWRLPR Q*VPILKRHLRPRSQRPLSKLRQLHPHHPPKSSQ NPRSAHSHPRKNQDVVLRQSLSLMAHGATRWKE VPSVPRHTPSLPSSMKTSR*PAVGLTTWTLTTL SLWIPFRPWSLVPQTLRIRRAK*THGGSPRIPSP SLSLHCPGRSACKPVTLMVLLPQAIPRPWPLPQ MHIARVPAVLLVPLSELKNRGRLP*KRNRPPRN PQRPPQ*RRRNRSQMKRALSPVGRI*HLRRKRNL PRRKVLAQPYWRRRPLSPLWGPKLPALWTQRVQ KGLSPRLLEVAECRTHPLSGGKRCLLPRPRRQG R*PHRIAGGKRTLQPKGSP*GWSLTILRTRVVGT TSRKTPLLPKR*AKSQLPKCP*GGQR*KRHPRN LTTLLPHLPDPLLNPMTSPLLKVLTPLILTSGM TPILTLFLPPQKCRSLPNCPNNHTTLTQTPVMSP LTPLRHPLRPPAHLLNPQPPLRSQPVLWKPMEW TGMG*TSPPRRRRRP*RLTHLG*KSRQNGLLSL IHLPRTPPQLLHQKHHQ*SLRWSTPQMRKSWRSP TRSGRA*QWT*RLTNRTTRSPRTCPPL*TRPNS VHPLRSWITETPMKLNIWRKLAPPYLRTTMPRR SRPCTLCLTLLRRALSSHLPSACQSPRRRVQGQV LKRLKPL*TLLRKTSILSHEDWPLTKSHTCRCQ RNPPRRSWRPWAWAPLQKRLKLQLPRAPLPLLT PSSAG*LTPSLSVVHLTIWSPT*QKRTPHYSLRN SRERLLTQQTSPSPKQPCTPASGPLRWRNLQAF CSSSPTWTLPSRSPEQRS*PRRERSQNGKINMK KAGGK*WK*GK*WPSMRRPSLR**RTNRERSQSP TRRCSSWFWRRSKPWPT*TPWRSLWPTSSEDMR R*RRS*KASARMKRC*RDVRRSTCPG*RRRSRG TRP*RCTRRRNWTGPMLRLLRFEARPSRSKPPTR PACGRSSCEWTPWKGRWSRRIKK*KNSPRFVTN *LPKWGKANSEPNVLDLTVACNMTVGTLLFLQF HGQVLFSLFRMHYCISFLNKIDLIVCSTKETIRI SCYWFAFS*YNS*QVDLRVPVSGRLSDSLIKDT LLTLALPFVHKFPG*AAFGFNMNMYSVHRDSCL KECKLDLHLLICF*KNKKCMFQIKFSIVNKIFSL DLG

Frame 1

RGV*EKSLSDHTGAHAHTRTPRAEPRTPATLGR APATLARTHTRRSPPGPRLGPRVAAPCRRSRRH GCPQLLPSAVQQPRPPAGSPRLPAAGIAPGRWPR SRLACTPGHSEDGRVAVPAASPSQRPEPGGFRG NEFRF*RGI*DPGVNDPCQSSASSTPTTPRSHP RTRGQHTATPGRTRMWF*DSPCP*WPTERLGGRK SLPSPVTLLLCRLR*RQADSQQWDLQLGL*QH* ACGYLSDLGASCLRR*ESGGQSEHTEEVHGFRP HL*VYTVPVAQPASQ*L*WCFFLRQSRGRGPCPR CI*HGFQQCF*YP*AN*KTEAAFLKKETDHQET HRDPPSEGDATGAR*REPCPQWGESSI*DENGI CQDGRS*PSLIGGDAP*ARCGAQSCLPSGLRECR RGCPPGFWRWQSAELTPCREENAASYHGPGGRG GNPIG*RGARGLSSQRALRKAGV*LF*GQE*LG QPAGKPPSYQKDRQKASCQNAPEEAKDEKDTRET *QHSCLTSQIPC*TQ*HPHC*RYLHL*Y*QVG* PQF*PFFFHLKNAGVSQTAPTIIQL*PRHL**V R*PL*DIL*DPQLTF*IPSLL*DPSQCYGSQWSG RGWAKQARQEEEDAPKD*HI*GEKVAKTVSSL* STFPGPHPSCYTRNTTSDLCGGPRHR*GKAGGH QPEVDVHDSGPRG*QTGLPAALGPVHLCKRDQIQ FTH*GVGLQKLL*N*IYGENWLLLTSGRRCPEE AGLVPYV*HFSGEPCQVISRPHVRVPDAVFRVK F*RD*SPCEHCCEKPASCPTRTGP*PRVTLAGAR EILPEGAGGHGLGHPFRSD*NYSSRGLLCLC*R PPQQASSPRLSLWCT*LSGARLSRKEPPTIRSE TPERGCSPNRRLHLQNSLVLPHRDR*GGETCRPS VPAARPGLCPPDRQSRDHNQGERGLRMER*I*R KQAGSDGNEENSGRV*EDHRSDDRGRTEREVSL PPDGAAAGSGEGASPGRPELRGEVSGRPLQKI*E DEGGPRRLPQE*RGVEEMCAGVPVPGEEGGAEV PGPEGARGGETGQGQC*DCSGSRQGPAGASRPP GQPAEGAAASGRPGKDAGAEE*RNRRTHQDL*RT DCQNGEKLTLNRMFWT*LLRAI*PSAHCCSSSS MDRFCFHFFVCTTVFPF*IKLI*LYAVLRRLSE FLAIGLHFPSIIHSKLTSEFLYQGDCLIL**KTH C*PWPCPLYTSSQGEQLLDLI*TCTACIGTLAL RSVNLICIC*FVFKKTRNACFK*NSLL*IKFFL WILA

Frame 2

GGFKRRAFRTTPALTLIPARPGQSRARRPHSGA RRPHSRAHIRGARPPAPGSGRESQLPAAAPAAT DARSCSPLQCSNPGRRPARPGSRLQESRQDAGP ARG*LARQGTARMGGSQSLQPAPASDLNLEASEA MSSDSEEAFETPESTTPVKAPPAPPPPPPEVIP EPEVSTQPPPEEPGCGSETVPVPDGPRSDSVEG SPFRPPSHSFSAVFDEDKPIASSGTYNLDFDNIE LVDTFQTLEPRASDAKNQEGKVNTRRKSTDSVP ISKSTLSRSLSLQASDFDGASSSGNPEAVALAP DAYSTGSSSASSTLKRTKKPRPPSLKKKQTTKKP TETPPVKETQQEPDEESLVPSGENLASETKTES AKTEGPSPALLEETPLEPAVGPKAACPLDSESA EGVVPPASGGGRVQNSPPVGRKTLPLTTAPEAGE VTPSDSGGQEDSPAKGLSVRLEFDYSEDKSSWD NQQENPPPTKKIGKKPVAKMPLRRPKMKKTPEK LDNTPASPPRSPAEPNDIPIAKGTYTFDIDKWDD PNFNPFSSTSKMQESPKLPQQSYNFDPDTCDES VDPFKTSSKTPSSPSKSPASFEIPASAMEANGV DGDGLNKPAKKKKTPLKTDTFRVKKSPKRSPLSD PPSQDPTPAATPETPPVISAVVHATDEEKLAVT NQKWTCMTVDLEADKQDYPQPSDLSTFVNETKF SSPTEELDYRNSYEIEYMEKIGSSLPQDDDAPKK QALYLMFDTSQESPVKSSPVRMSESPTPCSGSS FEETEALVNTAAKNQHPVPRGLAPNQESHLQVP EKSSQKELEAMGLGTPSEAIEITAPEGSFASADA LLSRLAHPVSLCGALDYLEPDLAEKNPPLFAQK LQREAAHPTDVSISKTALYSRIGTAEVEKPAGL LFQQPDLDSALQIARAEIITKEREVSEWKDKYEE SRREVMEMRKIVAEYEKTIAQMIEDEQREKSVS HQTVQQLVLEKEQALADLNSVEKSLADLFRRYE KMKEVLEGFRKNEEVLKRCAQEYLSRVKKEEQRY QALKVHAEEKLDRANAEIAQVRGKAQQEQAAHQ ASLRKEQLRVDALERTLEQKNKEIEELTKICDE LIAKMGKS*L*TECFGLNCCVQYDRRHTAVPPVP WTGSVFTFSYALLYFLSK*N*FDCMQY*GDYQN FLLLVCIFLV*FIAS*PQSSCIREIV*FSNKRH IADLGLALCTQVPRVSSFWI*YEHVQRA*GLLP* GV*T*SAFADLFLKKQEMHVSNKILYCK*NFFF GSWQ

Sequence NM_206862

GCCTGCTCCAAGGGAAGGATCAGGAGAGAAGAAACGCAAATCCCAGAACCGTGCCAACATATAAAACCCCACATTAAGGGTTGTACAGTGCACTGGGATT TCTCAAGTCACCCGCTTGGTCCTCTTCCAAGTATACTTTACTTCCTTTCATTCCTCTCTAAAACTTTTTTAAAAACTTTCACTCCTGCTCTAAAAGTTAT CTTGGTTTCTTACTCTACCTTATGCCCCTTGGGCGAATTTTTTCCTCTGAGGAGGGAAGAATAGAGTTGCTGCTGCAGACACATCAGATTCCCTACTGGT AACAGCTGGAGTGCGTCACCTCTGACAAAATTCTGGGGACGCTGGGAACACTGAATCAACATGGGCAATGAGAACAGCACCTCGGACAACCAGAGGACTT TATCAGCTCAGACTCCAAGGTCCGCGCAGCCACCCGGGAACAGTCAGAATATAAAAAGGAAGCAGCAGGACACGCCCGGAAGCCCTGACCACAGAGACGC GTCCAGCATTGGCAGCGTTGGGCTTGGAGGCTTCTGCACCGCTTCTGAGAGTTCTGCCAGCCTGGATCCATGCCTTGTGTCCCCAGAGGTGACTGAGCCA AGGAAGGACCCACAGGGAGCCAGGGGGCCAGAAGGTTCTTTGCTGCCCAGCCCACCACCGTCCCAGGAGCGAGAGCACCCCTCGTCCTCCATGCCCTTTG CCGAGTGTCCCCCGGAAGGTTGCTTGGCAAGTCCAGCAGCGGCACCTGAAGATGGTCCTCAGACTCAGTCTCCCAGGAGGGAACCTGCCCCAAATGCCCC AGGAGACATCGCGGCGGCATTTCCCGCTGAGAGGGACAGCTCTACTCCATACCAAGAGATTGCTGCCGTCCCCAGTGCTGGAAGAGAGAGACAGCCGAAG GAAGAAGGACAGAAGTCCTCCTTCTCCTTCTCCAGTGGCATCGACCAGTCACCTGGAATGTCGCCAGTACCCCTCAGAGAGCCAATGAAGGCACCGCTGT GTGGAGAGGGGGACCAGCCTGGTGGTTTTGAGTCCCAAGAGAAAGAGGCTGCAGGTGGCTTTCCCCCTGCAGAGTCCAGGCAGGGGGTGGCTTCTGTGCA AGTGACCCCTGAGGCCCCTGCTGCAGCCCAGCAGGGCACAGAAAGCTCAGCGGTCTTGGAGAAGTCCCCCCTAAAACCCATGGCCCCGATCCCACAAGAT CCAGCCCCAAGAGCCTCAGACAGAGAAAGAGGCCAAGGGGAGGCGCCGCCTCAGTATTTAACAGATGACTTGGAATTCCTCAGGGCCTGCCATCTCCCTA GGAGCAATTCAGGGGCTGCCCCAGAAGCAGAAGTGAATGCCGCTTCCCAGGAGAGCTGCCAGCAGCCAGTGGGAGCATATCTGCCGCACGCAGAGCTGCC CTGGGGCTTGCCAAGTCCTGCCCTGGTGCCAGAGGCTGGGGGCTCTGGGAAGGAGGCTCTGGACACCATTGATGTTCAGGGTCACCCACAGACAGGGATG CGAGGAACCAAGCCCAATCAAGTTGTCTGTGTGGCAGCAGGCGGCCAGCCCGAAGGGGGTTTGCCTGTGAGCCCTGAACCTTCCCTGCTCACTCCGACTG AGGAAGCACATCCAGCTTCAAGCCTCGCTTCATTCCCAGCTGCTCAGATTCCTATTGCTGTAGAAGAACCTGGATCATCATCCAGGGAATCAGTTTCCAA GGCTGGGATGCCAGTTTCTGCAGATGCAGCCAAAGAGGTGGTGGATGCAGGGTTGGTGGGACTGGAGAGGCAGGTGTCAGATCTTGGAAGCAAGGGAGAG CATCCAGAAGGGGACCCTGGAGAGGTTCCTGCCCCATCACCCCAGGAGAGGGGAGAGCACTTGAACACGGAGCAAAGCCATGAGGTCCAACCAGGAGTAC CACCCCCTCCTCTTCCCAAGGAGCAAAGCCATGAGGTCCAACCAGGAGCACCACCCCCTCCTCTTCCCAAGGCACCAAGTGAAAGTGCCAGAGGGCCACC GGGGCCAACGGATGGAGCCAAGGTCCATGAAGATTCCACAAGCCCAGCCGTGGCTAAAGAAGGAAGCAGATCACCTGGTGACAGCCCTGGAGGAAAGGAG GAAGCCCCAGAGCCACCTGATGGTGGAGACCCAGGGAACCTGCAAGGAGAGGACTCTCAGGCTTTCAGCAGCAAGCGTGATCCAGAAGTAGGCAAAGATG AGCTTTCAAAGCCAAGCAGTGATGCAGAGAGCAGAGACCATCCCAGCTCACACTCAGCACAGCCACCCAGAAAGGGGGGTGCTGGGCACACGGACGGGCC CCACTCTCAGACAGCAGAGGCTGATGCATCTGGCCTACCACACAAGCTGGGTGAGGAGGACCCCGTCCTGCCCCCTGTGCCAGATGGAGCTGGTGAGCCC ACTGTTCCCGAAGGAGCCATCTGGGAGGGGTCAGGATTGCAGCCCAAATGTCCTGACACCCTTCAGAGCAGGGAAGGATTGGGAAGAATGGAGTCTTTCC TGACTTTAGAATCAGAGAAATCAGATTTTCCACCAACTCCTGTTGCAGAGGTTGCACCCAAAGCCCAGGAAGGTGAGAGCACATTGGAAATAAGGAAGAT GGGCAGCTGTGATGGGGAGGGCTTGCTGACGTCCCCAGATCAACCCCGCGGGCCGGCGTGTGATGCGTCGAGACAGGAATTTCATGCTGGGGTGCCACAT CCCCCCCAGGGGGAGAACTTGGCAGCAGACCTGGGGCTCACGGCACTCATCCTGGACCAAGATCAGCAGGGAATCCCATCCTGCCCAGGGGAAGGCTGGA TAAGAGGAGCTGCATCCGAGTGGCCCCTACTATCTTCTGAGAAGCATCTCCAGCCATCCCAGGCACAACCAGAGACATCCATCTTTGACGTGCTCAAGGA GCAGGCCCAGCCACCTGAAAATGGGAAAGAGACTTCTCCAAGCCATCCAGGTTTTAAGGACCAGGGAGCAGATTCTTCCCAAATCCATGTACCTGTGGAA CCTCAGGAAGATAACAACTTGCCCACTCATGGAGGACAGGAGCAGGCTTTGGGATCAGAACTTCAAAGTCAGCTCCCCAAAGGCACCCTGTCTGATACTC CAACTTCATCTCCCACTGACATGGTTTGGGAGAGTTCTCTGACAGAAGAGTCAGAATTGTCAGCACCAACGAGACAGAAGTTGCCTGCACTAGGGGAGAA GCGGCCAGAGGGAGCATGCGGTGATGGTCAGTCCTCGAGGGTCTCGCCTCCAGCAGCAGATGTCTTAAAAGACTTTTCTCTTGCAGGGAACTTCAGCAGA AAGGAAACTTGCTGCACTGGGCAGGGGCCAAACAAGTCTCAACAGGCATTGGCTGATGCCTTGGAAGAAGGCAGCCAGCATGAAGAAGCATGTCAAAGGC ATCCAGGAGCTTCTGAAGCAGCTGATGGTTGTTCCCCACTCTGGGGCTTGAGTAAGAGGGAGATGGCAAGTGGAAACACAGGGGAGGCCCCACCTTGTCA GCCTGACTCAGTAGCTCTCCTGGATGCAGTTCCCTGCCTGCCAGCCCTGGCGCCCGCCAGCCCCGGAGTCACACCCACCCAGGATGCCCCAGAGACAGAG GCATGTGATGAAACCCAGGAAGGCAGGCAGCAACCAGTGCCGGCCCCGCAGCAGAAAATGGAGTGCTGGGCCACTTCGGATGCAGAGTCCCCAAAGCTTC TTGCAAGTTTCCCATCAGCTGGGGAGCAAGGTGGTGAAGCCGGGGCTGCTGAGACTGGTGGCAGCGCTGGTGCAGGAGACCCAGGAAAGCAGCAGGCTCC GGAGAAACCTGGAGAAGCTACTTTGAGTTGTGGCCTCCTTCAGACTGAGCACTGCCTTACCTCCGGGGAGGAAGCTTCTACCTCTGCCCTACGTGAGTCC TGCCAAGCTGAGCACCCCATGGCCAGCTGCCAGGATGCCTTGCTGCCAGCCAGAGAGCTGGGTGGGATTCCCAGGAGCACCATGGATTTTTCTACACACC AGGCTGTCCCAGACCCAAAGGAGCTCCTGCTGTCTGGGCCACCAGAAGTGGCTGCTCCTGACACCCCTTACCTGCATGTCGACAGTGCTGCCCAGAGAGG AGCAGAAGACAGTGGAGTGAAAGCTGTTTCCTCTGCAGACCCCAGAGCTCCTGGCGAAAGCCCCTGTCCTGTAGGGGAGCCCCCACTTGCCTTGGAAAAT GCTGCCTCCTTGAAGCTGTTTGCTGGCTCCCTCGCCCCCCTGTTGCAACCAGGAGCTGCAGGTGGGGAAATCCCTGCAGTGCAAGCCAGCAGTGGTAGTC CCAAAGCCAGAACCACTGAGGGACCAGTGGACTCCATGCCATGCCTGGACCGGATGCCACTTCTGGCCAAGGGCAAGCAGGCAACAGGGGAAGAGAAAGC AGCAACAGCTCCAGGTGCAGGTGCCAAGGCCAGTGGGGAGGGCATGGCAGGTGATGCAGCAGGAGAGACAGAGGGCAGCATGGAGAGGATGGGAGAGCCT TCCCAGGACCCAAAGCAGGGCACATCAGGTGGTGTGGACACAAGCTCTGAGCAAATCGCCACCCTCACTGGCTTCCCAGACTTCAGGGAGCACATCGCCA AGATCTTCGAGAAGCCTGTGCTCGGAGCCCTGGCCACACCTGGAGAAAAGGCAGGAGCTGGGAGGAGTGCAGTGGGTAAAGACCTCACCAGGCCATTGGG CCCAGAGAAGCTTCTAGATGGGCCTCCAGGAGTGGATGTCACCCTTCTCCCTGCACCTCCTGCTCGACTCCAGGTGGAGAAGAAGCAACAGTTGGCTGGA GAGGCTGAGATTTCCCATCTGGCTCTGCAAGATCCAGCTTCAGACAAGCTTCTGGGTCCAGCAGGGCTGACCTGGGAGCGGAACTTGCCAGGTGCCGGTG TGGGGAAGGAGATGGCAGGTGTCCCACCCACACTGAGGGAAGACGAGAGGCCAGAGGGGCCTGGGGCAGCCTGGCCAGGCCTGGAAGGCCAGGCTTACTC ACAGCTGGAGAGGAGCAGGCAGGAATTAGCTTCAGGTCTTCCTTCACCAGCAGCTACTCAGGAGCTCCCTGTGGAGAGAGCTGCTGCCTTCCAGGTGGCT CCCCATAGCCATGGAGAAGAGGCCGTGGCCCAAGACAGAATTCCTTCTGGAAAGCAGCACCAGGAAACATCTGCCTGCGACAGTCCACATGGAGAAGATG GTCCCGGGGACTTTGCTCACACAGGGGTTCCAGGACATGTGCCAAGGTCCACGTGTGCCCCTTCTCCTCAGAGGGAGGTTTTGACTGTGCCTGAGGCCAA CAGTGAGCCCTGGACCCTTGACACGCTTGGGGGTGAAAGGAGACCCGGAGTCACTGCTGGCATCTTGGAAATGCGAAATGCCCTGGGCAACCAGAGCACC CCTGCACCACCAACTGGAGAAGTGGCAGACACTCCCCTGGAGCCTGGCAAGGTGGCAGGCGCTGCTGGGGAAGCAGAGGGTGACATCACCCTGAGCACAG CTGAGACACAGGCATGTGCGTCCGGTGATCTGCCTGAAGCAGGTACTACGAGGACATTCTCCGTTGTGGCAGGTGACTTGGTGCTGCCAGGAAGCTGTCA GGACCCAGCCTGCTCTGACAAGGCTCCGGGGATGGAGGGTACAGCTGCCCTTCATGGGGACAGCCCAGCCAGGCCCCAGCAGGCTAAGGAGCAGCCAGGG CCTGAGCGCCCCATTCCAGCTGGGGATGGGAAGGTGTGCGTCTCCTCACCTCCAGAGCCTGACGAAACTCACGACCCGAAGCTGCAACATTTGGCTCCAG AAGAGCTCCACACTGACAGAGAGAGCCCCAGGCCTGGCCCATCCATGTTACCTTCGGTTCCTAAGAAGGATGCTCCAAGAGTCATGGATAAAGTCACTTC AGATGAGACCAGAGGTGCGGAAGGAACAGAAAGTTCACCTGTGGCAGATGATATCATCCAGCCCGCTGCCCCCGCAGACCTGGAAAGCCCAACCTTAGCT GCCTCTTCCTACCACGGTGATGTTGTTGGCCAGGTCTCTACGGATCTGATAGCCCAGAGCATCTCCCCAGCTGCTGCCCATGCGGGTCTTCCTCCCTCGG CTGCAGAACACATAGTTTCGCCATCTGCCCCAGCTGGTGACAGAGTAGAAGCTTCCACTCCCTCCTGCCCAGATCCGGCCAAGGACCTCAGCAGGAGTTC CGATTCTGAAGAGGCATTTGAGACCCCGGAGTCAACGACCCCTGTCAAAGCTCCGCCAGCTCCACCCCCACCACCCCCCGAAGTCATCCCAGAACCCGAG GTCAGCACACAGCCACCCCCGGAAGAACCAGGATGTGGTTCTGAGACAGTCCCTGTCCCTGATGGCCCACGGAGCGACTCGGTGGAAGGAAGTCCCTTCC GTCCCCCGTCACACTCCTTCTCTGCCGTCTTCGATGAAGACAAGCCGATAGCCAGCAGTGGGACTTACAACTTGGACTTTGACAACATTGAGCTTGTGGA TACCTTTCAGACCTTGGAGCCTCGTGCCTCAGACGCTAAGAATCAGGAGGGCAAAGTGAACACACGGAGGAAGTCCACGGATTCCGTCCCCATCTCTAAG TCTACACTGTCCCGGTCGCTCAGCCTGCAAGCCAGTGACTTTGATGGTGCTTCTTCCTCAGGCAATCCCGAGGCCGTGGCCCTTGCCCCAGATGCATATA GCACGGGTTCCAGCAGTGCTTCTAGTACCCTTAAGCGAACTAAAAAACCGAGGCCGCCTTCCTTAAAAAAGAAACAGACCACCAAGAAACCCACAGAGAC CCCCCCAGTGAAGGAGACGCAACAGGAGCCAGATGAAGAGAGCCTTGTCCCCAGTGGGGAGAATCTAGCATCTGAGACGAAAACGGAATCTGCCAAGACG GAAGGTCCTAGCCCAGCCTTATTGGAGGAGACGCCCCTTGAGCCCGCTGTGGGGCCCAAAGCTGCCTGCCCTCTGGACTCAGAGAGTGCAGAAGGGGTTG TCCCCCCGGCTTCTGGAGGTGGCAGAGTGCAGAACTCACCCCCTGTCGGGAGGAAAACGCTGCCTCTTACCACGGCCCCGGAGGCAGGGGAGGTAACCCC ATCGGATAGCGGGGGGCAAGAGGACTCTCCAGCCAAAGGGCTCTCCGTAAGGCTGGAGTTTGACTATTCTGAGGACAAGAGTAGTTGGGACAACCAGCAG GAAAACCCCCCTCCTACCAAAAAGATAGGCAAAAAGCCAGTTGCCAAAATGCCCCTGAGGAGGCCAAAGATGAAAAAGACACCCGAGAAACTTGACAACA CTCCTGCCTCACCTCCCAGATCCCCTGCTGAACCCAATGACATCCCCATTGCTAAAGGTACTTACACCTTTGATATTGACAAGTGGGATGACCCCAATTT TAACCCTTTTTCTTCCACCTCAAAAATGCAGGAGTCTCCCAAACTGCCCCAACAATCATACAACTTTGACCCAGACACCTGTGATGAGTCCGTTGACCCC TTTAAGACATCCTCTAAGACCCCCAGCTCACCTTCTAAATCCCCAGCCTCCTTTGAGATCCCAGCCAGTGCTATGGAAGCCAATGGAGTGGACGGGGATG GGCTAAACAAGCCCGCCAAGAAGAAGAAGACGCCCCTAAAGACTGACACATTTAGGGTGAAAAAGTCGCCAAAACGGTCTCCTCTCTCTGATCCACCTTC CCAGGACCCCACCCCAGCTGCTACACCAGAAACACCACCAGTGATCTCTGCGGTGGTCCACGCCACAGATGAGGAAAAGCTGGCGGTCACCAACCAGAAG TGGACGTGCATGACAGTGGACCTAGAGGCTGACAAACAGGACTACCCGCAGCCCTCGGACCTGTCCACCTTTGTAAACGAGACCAAATTCAGTTCACCCA CTGAGGAGTTGGATTACAGAAACTCCTATGAAATTGAATATATGGAGAAAATTGGCTCCTCCTTACCTCAGGACGACGATGCCCCGAAGAAGCAGGCCTT GTACCTTATGTTTGACACTTCTCAGGAGAGCCCTGTCAAGTCATCTCCCGTCCGCATGTCAGAGTCCCCGACGCCGTGTTCAGGGTCAAGTTTTGAAGAG ACTGAAGCCCTTGTGAACACTGCTGCGAAAAACCAGCATCCTGTCCCACGAGGACTGGCCCCTAACCAAGAGTCACACTTGCAGGTGCCAGAGAAATCCT CCCAGAAGGAGCTGGAGGCCATGGGCTTGGGCACCCCTTCAGAAGCGATTGAAATTACAGCTCCCGAGGGCTCCTTTGCCTCTGCTGACGCCCTCCTCAG CAGGCTAGCTCACCCCGTCTCTCTCTGTGGTGCACTTGACTATCTGGAGCCCGACTTAGCAGAAAAGAACCCCCCACTATTCGCTCAGAAACTCCAGGAG GAGTTAGAGTTTGCCATCATGCGGATAGAAGCCCTGAAGCTGGCCAGGCAGATTGCTTTGGCTTCCCGCAGCCACCAGGATGCCAAGAGAGAGGCTGCTC ACCCAACAGACGTCTCCATCTCCAAAACAGCCTTGTACTCCCGCATCGGGACCGCTGAGGTGGAGAAACCTGCAGGCCTTCTGTTCCAGCAGCCCGACCT GGACTCTGCCCTCCAGATCGCCAGAGCAGAGATCATAACCAAGGAGAGAGAGGTCTCAGAATGGAAAGATAAATATGAAGAAAGCAGGCGGGAAGTGATG GAAATGAGGAAAATAGTGGCCGAGTATGAGAAGACCATCGCTCAGATGATAGAGGACGAACAGAGAGAGAAGTCAGTCTCCCACCAGACGGTGCAGCAGC TGGTTCTGGAGAAGGAGCAAGCCCTGGCCGACCTGAACTCCGTGGAGAAGTCTCTGGCCGACCTCTTCAGAAGATATGAGAAGATGAAGGAGGTCCTAGA AGGCTTCCGCAAGAATGAAGAGGTGTTGAAGAGATGTGCGCAGGAGTACCTGTCCCGGGTGAAGAAGGAGGAGCAGAGGTACCAGGCCCTGAAGGTGCAC GCGGAGGAGAAACTGGACAGGGCCAATGCTGAGATTGCTCAGGTTCGAGGCAAGGCCCAGCAGGAGCAAGCCGCCCACCAGGCCAGCCTGCGGAAGGAGC AGCTGCGAGTGGACGCCCTGGAAAGGACGCTGGAGCAGAAGAATAAAGAAATAGAAGAACTCACCAAGATTTGTGACGAACTGATTGCCAAAATGGGGAA AAGCTAACTCTGAACCGAATGTTTTGGACTTAACTGTTGCGTGCAATATGACCGTCGGCACACTGCTGTTCCTCCAGTTCCATGGACAGGTTCTGTTTTC ACTTTTTCGTATGCACTACTGTATTTCCTTTCTAAATAAAATTGATTTGATTGTATGCAGTACTAAGGAGACTATCAGAATTTCTTGCTATTGGTTTGCA TTTTCCTAGTATAATTCATAGCAAGTTGACCTCAGAGTTCCTGTATCAGGGAGATTGTCTGATTCTCTAATAAAAGACACATTGCTGACCTTGGCCTTGC CCTTTGTACACAAGTTCCCAGGGTGAGCAGCTTTTGGATTTAATATGAACATGTACAGCGTGCATAGGGACTCTTGCCTTAAGGAGTGTAAACTTGATCT GCATTTGCTGATTTGTTTTTAAAAAAACAAGAAATGCATGTTTCAAATAAAATTCTCTATTGTAAATAAAATTTTTTCTTTGGATCTTGGCAA

refseq_TACC2.F1 (forward primer)
refseq_TACC2.R1 (reverse primer)
Single exon skipping

Frame 0

ACSKGRIRREETQIPEPCQHIKPHIKGCTVHWD FSSHPLGPLPSILYFLSFLSKTFLKTFTPALKVI LVSYSTLCPLGEFFPLRREE*SCCCRHIRFPTG NSWSASPLTKFWGRWEH*INMGNENSTSDNQRT LSAQTPRSAQPPGNSQNIKRKQQDTPGSPDHRDA SSIGSVGLGGFCTASESSASLDPCLVSPEVTEP RKDPQGARGPEGSLLPSPPPSQEREHPSSSMPF AECPPEGCLASPAAAPEDGPQTQSPRREPAPNAP GDIAAAFPAERDSSTPYQEIAAVPSAGRERQPK EEGQKSSFSFSSGIDQSPGMSPVPLREPMKAPL CGEGDQPGGFESQEKEAAGGFPPAESRQGVASVQ VTPEAPAAAQQGTESSAVLEKSPLKPMAPIPQD PAPRASDRERGQGEAPPQYLTDDLEFLRACHLP RSNSGAAPEAEVNAASQESCQQPVGAYLPHAELP WGLPSPALVPEAGGSGKEALDTIDVQGHPQTGM RGTKPNQVVCVAAGGQPEGGLPVSPEPSLLTPT EEAHPASSLASFPAAQIPIAVEEPGSSSRESVSK AGMPVSADAAKEVVDAGLVGLERQVSDLGSKGE HPEGDPGEVPAPSPQERGEHLNTEQSHEVQPGV PPPPLPKEQSHEVQPGAPPPPLPKAPSESARGPP GPTDGAKVHEDSTSPAVAKEGSRSPGDSPGGKE EAPEPPDGGDPGNLQGEDSQAFSSKRDPEVGKD ELSKPSSDAESRDHPSSHSAQPPRKGGAGHTDGP HSQTAEADASGLPHKLGEEDPVLPPVPDGAGEP TVPEGAIWEGSGLQPKCPDTLQSREGLGRMESF LTLESEKSDFPPTPVAEVAPKAQEGESTLEIRKM GSCDGEGLLTSPDQPRGPACDASRQEFHAGVPH PPQGENLAADLGLTALILDQDQQGIPSCPGEGW IRGAASEWPLLSSEKHLQPSQAQPETSIFDVLKE QAQPPENGKETSPSHPGFKDQGADSSQIHVPVE PQEDNNLPTHGGQEQALGSELQSQLPKGTLSDT PTSSPTDMVWESSLTEESELSAPTRQKLPALGEK RPEGACGDGQSSRVSPPAADVLKDFSLAGNFSR KETCCTGQGPNKSQQALADALEEGSQHEEACQR HPGASEAADGCSPLWGLSKREMASGNTGEAPPCQ PDSVALLDAVPCLPALAPASPGVTPTQDAPETE ACDETQEGRQQPVPAPQQKMECWATSDAESPKL LASFPSAGEQGGEAGAAETGGSAGAGDPGKQQAP EKPGEATLSCGLLQTEHCLTSGEEASTSALRES CQAEHPMASCQDALLPARELGGIPRSTMDFSTH QAVPDPKELLLSGPPEVAAPDTPYLHVDSAAQRG AEDSGVKAVSSADPRAPGESPCPVGEPPLALEN AASLKLFAGSLAPLLQPGAAGGEIPAVQASSGS PKARTTEGPVDSMPCLDRMPLLAKGKQATGEEKA ATAPGAGAKASGEGMAGDAAGETEGSMERMGEP SQDPKQGTSGGVDTSSEQIATLTGFPDFREHIA KIFEKPVLGALATPGEKAGAGRSAVGKDLTRPLG PEKLLDGPPGVDVTLLPAPPARLQVEKKQQLAG EAEISHLALQDPASDKLLGPAGLTWERNLPGAG VGKEMAGVPPTLREDERPEGPGAAWPGLEGQAYS QLERSRQELASGLPSPAATQELPVERAAAFQVA PHSHGEEAVAQDRIPSGKQHQETSACDSPHGED GPGDFAHTGVPGHVPRSTCAPSPQREVLTVPEAN SEPWTLDTLGGERRPGVTAGILEMRNALGNQST PAPPTGEVADTPLEPGKVAGAAGEAEGDITLST AETQACASGDLPEAGTTRTFSVVAGDLVLPGSCQ DPACSDKAPGMEGTAALHGDSPARPQQAKEQPG PERPIPAGDGKVCVSSPPEPDETHDPKLQHLAP EELHTDRESPRPGPSMLPSVPKKDAPRVMDKVTS DETRGAEGTESSPVADDIIQPAAPADLESPTLA ASSYHGDVVGQVSTDLIAQSISPAAAHAGLPPS AAEHIVSPSAPAGDRVEASTPSCPDPAKDLSRSS DSEEAFETPESTTPVKAPPAPPPPPPEVIPEPE VSTQPPPEEPGCGSETVPVPDGPRSDSVEGSPF RPPSHSFSAVFDEDKPIASSGTYNLDFDNIELVD TFQTLEPRASDAKNQEGKVNTRRKSTDSVPISK STLSRSLSLQASDFDGASSSGNPEAVALAPDAY STGSSSASSTLKRTKKPRPPSLKKKQTTKKPTET PPVKETQQEPDEESLVPSGENLASETKTESAKT EGPSPALLEETPLEPAVGPKAACPLDSESAEGV VPPASGGGRVQNSPPVGRKTLPLTTAPEAGEVTP SDSGGQEDSPAKGLSVRLEFDYSEDKSSWDNQQ ENPPPTKKIGKKPVAKMPLRRPKMKKTPEKLDN TPASPPRSPAEPNDIPIAKGTYTFDIDKWDDPNF NPFSSTSKMQESPKLPQQSYNFDPDTCDESVDP FKTSSKTPSSPSKSPASFEIPASAMEANGVDGD GLNKPAKKKKTPLKTDTFRVKKSPKRSPLSDPPS QDPTPAATPETPPVISAVVHATDEEKLAVTNQK WTCMTVDLEADKQDYPQPSDLSTFVNETKFSSP TEELDYRNSYEIEYMEKIGSSLPQDDDAPKKQAL YLMFDTSQESPVKSSPVRMSESPTPCSGSSFEE TEALVNTAAKNQHPVPRGLAPNQESHLQVPEKS SQKELEAMGLGTPSEAIEITAPEGSFASADALLS RLAHPVSLCGALDYLEPDLAEKNPPLFAQKLQE ELEFAIMRIEALKLARQIALASRSHQDAKREAA HPTDVSISKTALYSRIGTAEVEKPAGLLFQQPDL DSALQIARAEIITKEREVSEWKDKYEESRREVM EMRKIVAEYEKTIAQMIEDEQREKSVSHQTVQQ LVLEKEQALADLNSVEKSLADLFRRYEKMKEVLE GFRKNEEVLKRCAQEYLSRVKKEEQRYQALKVH AEEKLDRANAEIAQVRGKAQQEQAAHQASLRKE QLRVDALERTLEQKNKEIEELTKICDELIAKMGK S*L*TECFGLNCCVQYDRRHTAVPPVPWTGSVF TFSYALLYFLSK*N*FDCMQY*GDYQNFLLLVC IFLV*FIAS*PQSSCIREIV*FSNKRHIADLGLA LCTQVPRVSSFWI*YEHVQRA*GLLP*GV*T*S AFADLFLKKQEMHVSNKILYCK*NFFFGSWQ

Frame 1

PAPREGSGEKKRKSQNRANI*NPTLRVVQCTGI SQVTRLVLFQVYFTSFHSSLKLF*KLSLLL*KL SWFLTLPYAPWANFFL*GGKNRVAAADTSDSLLV TAGVRHL*QNSGDAGNTESTWAMRTAPRTTRGL YQLRLQGPRSHPGTVRI*KGSSRTRPEALTTET RPALAALGLEASAPLLRVLPAWIHALCPQR*LSQ GRTHREPGGQKVLCCPAHHRPRSESTPRPPCPL PSVPRKVAWQVQQRHLKMVLRLSLPGGNLPQMP QETSRRHFPLRGTALLHTKRLLPSPVLEERDSRR KKDRSPPSPSPVASTSHLECRQYPSESQ*RHRC VERGTSLVVLSPKRKRLQVAFPLQSPGRGWLLC K*PLRPLLQPSRAQKAQRSWRSPP*NPWPRSHKI QPQEPQTEKEAKGRRRLSI*QMTWNSSGPAISL GAIQGLPQKQK*MPLPRRAASSQWEHICRTQSC PGACQVLPWCQRLGALGRRLWTPLMFRVTHRQGC EEPSPIKLSVWQQAASPKGVCL*ALNLPCSLRL RKHIQLQASLHSQLLRFLLL*KNLDHHPGNQFP RLGCQFLQMQPKRWWMQGWWDWRGRCQILEARES IQKGTLERFLPHHPRRGEST*TRSKAMRSNQEY HPLLFPRSKAMRSNQEHHPLLFPRHQVKVPEGH RGQRMEPRSMKIPQAQPWLKKEADHLVTALEERR KPQSHLMVETQGTCKERTLRLSAASVIQK*AKM SFQSQAVMQRAETIPAHTQHSHPERGVLGTRTG PTLRQQRLMHLAYHTSWVRRTPSCPLCQMELVSP LFPKEPSGRGQDCSPNVLTPFRAGKDWEEWSLS *L*NQRNQIFHQLLLQRLHPKPRKVRAHWK*GR WAAVMGRAC*RPQINPAGRRVMRRDRNFMLGCHI PPRGRTWQQTWGSRHSSWTKISRESHPAQGKAG *EELHPSGPYYLLRSISSHPRHNQRHPSLTCSR SRPSHLKMGKRLLQAIQVLRTREQILPKSMYLWN LRKITTCPLMEDRSRLWDQNFKVSSPKAPCLIL QLHLPLTWFGRVL*QKSQNCQHQRDRSCLH*GR SGQREHAVMVSPRGSRLQQQMS*KTFLLQGTSAE RKLAALGRGQTSLNRHWLMPWKKAASMKKHVKG IQELLKQLMVVPHSGA*VRGRWQVETQGRPHLV SLTQ*LSWMQFPACQPWRPPAPESHPPRMPQRQR HVMKPRKAGSNQCRPRSRKWSAGPLRMQSPQSF LQVSHQLGSKVVKPGLLRLVAALVQETQESSRL RRNLEKLL*VVASFRLSTALPPGRKLLPLPYVSP AKLSTPWPAARMPCCQPESWVGFPGAPWIFLHT RLSQTQRSSCCLGHQKWLLLTPLTCMSTVLPRE EQKTVE*KLFPLQTPELLAKAPVL*GSPHLPWKM LPP*SCLLAPSPPCCNQELQVGKSLQCKPAVVV PKPEPLRDQWTPCHAWTGCHFWPRASRQQGKRK QQQLQVQVPRPVGRAWQVMQQERQRAAWRGWESL PRTQSRAHQVVWTQALSKSPPSLASQTSGSTSP RSSRSLCSEPWPHLEKRQELGGVQWVKTSPGHW AQRSF*MGLQEWMSPFSLHLLLDSRWRRSNSWLE RLRFPIWLCKIQLQTSFWVQQG*PGSGTCQVPV WGRRWQVSHPH*GKTRGQRGLGQPGQAWKARLT HSWRGAGRN*LQVFLHQQLLRSSLWRELLPSRWL PIAMEKRPWPKTEFLLESSTRKHLPATVHMEKM VPGTLLTQGFQDMCQGPRVPLLLRGRF*LCLRP TVSPGPLTRLGVKGDPESLLASWKCEMPWATRAP LHHQLEKWQTLPWSLARWQALLGKQRVTSP*AQ LRHRHVRPVICLKQVLRGHSPLWQVTWCCQEAV RTQPALTRLRGWRVQLPFMGTAQPGPSRLRSSQG LSAPFQLGMGRCASPHLQSLTKLTTRSCNIWLQ KSSTLTERAPGLAHPCYLRFLRRMLQESWIKSL QMRPEVRKEQKVHLWQMISSSPLPPQTWKAQP*L PLPTTVMLLARSLRI**PRASPQLLPMRVFLPR LQNT*FRHLPQLVTE*KLPLPPAQIRPRTSAGV PILKRHLRPRSQRPLSKLRQLHPHHPPKSSQNPR SAHSHPRKNQDVVLRQSLSLMAHGATRWKEVPS VPRHTPSLPSSMKTSR*PAVGLTTWTLTTLSLW IPFRPWSLVPQTLRIRRAK*THGGSPRIPSPSLS LHCPGRSACKPVTLMVLLPQAIPRPWPLPQMHI ARVPAVLLVPLSELKNRGRLP*KRNRPPRNPQR PPQ*RRRNRSQMKRALSPVGRI*HLRRKRNLPRR KVLAQPYWRRRPLSPLWGPKLPALWTQRVQKGL SPRLLEVAECRTHPLSGGKRCLLPRPRRQGR*P HRIAGGKRTLQPKGSP*GWSLTILRTRVVGTTSR KTPLLPKR*AKSQLPKCP*GGQR*KRHPRNLTT LLPHLPDPLLNPMTSPLLKVLTPLILTSGMTPI LTLFLPPQKCRSLPNCPNNHTTLTQTPVMSPLTP LRHPLRPPAHLLNPQPPLRSQPVLWKPMEWTGM G*TSPPRRRRRP*RLTHLG*KSRQNGLLSLIHL PRTPPQLLHQKHHQ*SLRWSTPQMRKSWRSPTRS GRA*QWT*RLTNRTTRSPRTCPPL*TRPNSVHP LRSWITETPMKLNIWRKLAPPYLRTTMPRRSRP CTLCLTLLRRALSSHLPSACQSPRRRVQGQVLKR LKPL*TLLRKTSILSHEDWPLTKSHTCRCQRNP PRRSWRPWAWAPLQKRLKLQLPRAPLPLLTPSS AG*LTPSLSVVHLTIWSPT*QKRTPHYSLRNSRR S*SLPSCG*KP*SWPGRLLWLPAATRMPRERLL TQQTSPSPKQPCTPASGPLRWRNLQAFCSSSPT WTLPSRSPEQRS*PRRERSQNGKINMKKAGGK*W K*GK*WPSMRRPSLR**RTNRERSQSPTRRCSS WFWRRSKPWPT*TPWRSLWPTSSEDMRR*RRS* KASARMKRC*RDVRRSTCPG*RRRSRGTRP*RCT RRRNWTGPMLRLLRFEARPSRSKPPTRPACGRS SCEWTPWKGRWSRRIKK*KNSPRFVTN*LPKWG KANSEPNVLDLTVACNMTVGTLLFLQFHGQVLFS LFRMHYCISFLNKIDLIVCSTKETIRISCYWFA FS*YNS*QVDLRVPVSGRLSDSLIKDTLLTLAL PFVHKFPG*AAFGFNMNMYSVHRDSCLKECKLDL HLLICF*KNKKCMFQIKFSIVNKIFSLDLG

Frame 2

LLQGKDQERRNANPRTVPTYKTPH*GLYSALGF LKSPAWSSSKYTLLPFIPL*NFFKNFHSCSKSY LGFLLYLMPLGRIFSSEEGRIELLLQTHQIPYW *QLECVTSDKILGTLGTLNQHGQ*EQHLGQPEDF ISSDSKVRAATREQSEYKKEAAGHARKP*PQRR VQHWQRWAWRLLHRF*EFCQPGSMPCVPRGD*A KEGPTGSQGARRFFAAQPTTVPGARAPLVLHALC RVSPGRLLGKSSSGT*RWSSDSVSQEGTCPKCP RRHRGGISR*EGQLYSIPRDCCRPQCWKRETAE GRRTEVLLLLLQWHRPVTWNVASTPQRANEGTAV WRGGPAWWF*VPRERGCRWLSPCRVQAGGGFCA SDP*GPCCSPAGHRKLSGLGEVPPKTHGPDPTR SSPKSLRQRKRPRGGAASVFNR*LGIPQGLPSP* EQFRGCPRSRSECRFPGELPAASGSISAARRAA LGLAKSCPGARGWGLWEGGSGHH*CSGSPTDRD ARNQAQSSCLCGSRRPARRGFACEP*TFPAHSD* GSTSSFKPRFIPSCSDSYCCRRTWIIIQGISFQ GWDASFCRCSQRGGGCRVGGTGEAGVRSWKQGR ASRRGPWRGSCPITPGEGRALEHGAKP*GPTRST TPSSSQGAKP*GPTRSTTPSSSQGTK*KCQRAT GANGWSQGP*RFHKPSRG*RRKQITW*QPWRKG GSPRAT*WWRPREPARRGLSGFQQQA*SRSRQR* AFKAKQ*CREQRPSQLTLSTATQKGGCWAHGRA PLSDSRG*CIWPTTQAG*GGPRPAPCARWSW*A HCSRRSHLGGVRIAAQMS*HPSEQGRIGKNGVFP DFRIREIRFSTNSCCRGCTQSPGR*EHIGNKED GQL*WGGLADVPRSTPRAGV*CVETGISCWGAT SPPGGELGSRPGAHGTHPGPRSAGNPILPRGRLD KRSCIRVAPTIF*EASPAIPGTTRDIHL*RAQG AGPAT*KWERDFSKPSRF*GPGSRFFPNPCTCG TSGR*QLAHSWRTGAGFGIRTSKSAPQRHPV*YS NFISH*HGLGEFSDRRVRIVSTNETEVACTRGE AARGSMR*WSVLEGLASSSRCLKRLFSCRELQQ KGNLLHWAGAKQVSTGIG*CLGRRQPA*RSMSKA SRSF*SS*WLFPTLGLE*EGDGKWKHRGGPTLS A*LSSSPGCSSLPASPGARQPRSHTHPGCPRDR GM**NPGRQAATSAGPAAENGVLGHFGCRVPKAS CKFPISWGARW*SRGC*DWWQRWCRRPRKAAGS GETWRSYFELWPPSD*ALPYLRGGSFYLCPT*V LPS*APHGQLPGCLAASQRAGWDSQEHHGFFYTP GCPRPKGAPAVWATRSGCS*HPLPACRQCCPER SRRQWSESCFLCRPQSSWRKPLSCRGAPTCLGK CCLLEAVCWLPRPPVATRSCRWGNPCSASQQW*S QSQNH*GTSGLHAMPGPDATSGQGQAGNRGRES SNSSRCRCQGQWGGHGR*CSRRDRGQHGEDGRA FPGPKAGHIRWCGHKL*ANRHPHWLPRLQGAHRQ DLREACARSPGHTWRKGRSWEECSG*RPHQAIG PREASRWASRSGCHPSPCTSCSTPGGEEATVGW RG*DFPSGSARSSFRQASGSSRADLGAELARCRC GEGDGRCPTHTEGRREARGAWGSLARPGRPGLL TAGEEQAGISFRSSFTSSYSGAPCGESCCLPGG SP*PWRRGRGPRQNSFWKAAPGNICLRQSTWRRW SRGLCSHRGSRTCAKVHVCPFSSEGGFDCA*GQ Q*ALDP*HAWG*KETRSHCWHLGNAKCPGQPEH PCTTNWRSGRHSPGAWQGGRRCWGSRG*HHPEHS *DTGMCVR*SA*SRYYEDILRCGR*LGAARKLS GPSLL*QGSGDGGYSCPSWGQPSQAPAG*GAAR A*APHSSWGWEGVRLLTSRA*RNSRPEAATFGSR RAPH*QREPQAWPIHVTFGS*EGCSKSHG*SHF R*DQRCGRNRKFTCGR*YHPARCPRRPGKPNLS CLFLPR*CCWPGLYGSDSPEHLPSCCPCGSSSLG CRTHSFAICPSW*QSRSFHSLLPRSGQGPQQEF RF*RGI*DPGVNDPCQSSASSTPTTPRSHPRTR GQHTATPGRTRMWF*DSPCP*WPTERLGGRKSLP SPVTLLLCRLR*RQADSQQWDLQLGL*QH*ACG YLSDLGASCLRR*ESGGQSEHTEEVHGFRPHL* VYTVPVAQPASQ*L*WCFFLRQSRGRGPCPRCI* HGFQQCF*YP*AN*KTEAAFLKKETDHQETHRD PPSEGDATGAR*REPCPQWGESSI*DENGICQD GRS*PSLIGGDAP*ARCGAQSCLPSGLRECRRGC PPGFWRWQSAELTPCREENAASYHGPGGRGGNP IG*RGARGLSSQRALRKAGV*LF*GQE*LGQPA GKPPSYQKDRQKASCQNAPEEAKDEKDTRET*QH SCLTSQIPC*TQ*HPHC*RYLHL*Y*QVG*PQF *PFFFHLKNAGVSQTAPTIIQL*PRHL**VR*P L*DIL*DPQLTF*IPSLL*DPSQCYGSQWSGRGW AKQARQEEEDAPKD*HI*GEKVAKTVSSL*STF PGPHPSCYTRNTTSDLCGGPRHR*GKAGGHQPE VDVHDSGPRG*QTGLPAALGPVHLCKRDQIQFTH *GVGLQKLL*N*IYGENWLLLTSGRRCPEEAGL VPYV*HFSGEPCQVISRPHVRVPDAVFRVKF*R D*SPCEHCCEKPASCPTRTGP*PRVTLAGAREIL PEGAGGHGLGHPFRSD*NYSSRGLLCLC*RPPQ QASSPRLSLWCT*LSGARLSRKEPPTIRSETPG GVRVCHHADRSPEAGQADCFGFPQPPGCQERGCS PNRRLHLQNSLVLPHRDR*GGETCRPSVPAARP GLCPPDRQSRDHNQGERGLRMER*I*RKQAGSD GNEENSGRV*EDHRSDDRGRTEREVSLPPDGAAA GSGEGASPGRPELRGEVSGRPLQKI*EDEGGPR RLPQE*RGVEEMCAGVPVPGEEGGAEVPGPEGA RGGETGQGQC*DCSGSRQGPAGASRPPGQPAEGA AASGRPGKDAGAEE*RNRRTHQDL*RTDCQNGE KLTLNRMFWT*LLRAI*PSAHCCSSSSMDRFCF HFFVCTTVFPF*IKLI*LYAVLRRLSEFLAIGLH FPSIIHSKLTSEFLYQGDCLIL**KTHC*PWPC PLYTSSQGEQLLDLI*TCTACIGTLALRSVNLI CIC*FVFKKTRNACFK*NSLL*IKFFLWILA

Sequence NM_206861

refseq_TACC2.F1 (forward primer)
refseq_TACC2.R1 (reverse primer)
Single exon skipping

Frame 0

ACSKGRIRREETQIPEPCQHIKPHIKGCTVHWD FSSHPLGPLPSILYFLSFLSKTFLKTFTPALKVI LVSYSTLCPLGEFFPLRREE*SCCCRHIRFPTG NSWSASPLTKFWGRWEH*INMGNENSTSDNQRT LSAQTPRSAQPPGNSQNIKRKQQDTPGSPDHRDA SSSPVADDIIQPAAPADLESPTLAASSYHGDVV GQVSTDLIAQRSSDSEEAFETPESTTPVKAPPA PPPPPPEVIPEPEVSTQPPPEEPGCGSETVPVPD GPRSDSVEGSPFRPPSHSFSAVFDEDKPIASSG TYNLDFDNIELVDTFQTLEPRASDAKNQEGKVN TRRKSTDSVPISKSTLSRSLSLQASDFDGASSSG NPEAVALAPDAYSTGSSSASSTLKRTKKPRPPS LKKKQTTKKPTETPPVKETQQEPDEESLVPSGE NLASETKTESAKTEGPSPALLEETPLEPAVGPKA ACPLDSESAEGVVPPASGGGRVQNSPPVGRKTL PLTTAPEAGEVTPSDSGGQEDSPAKGLSVRLEF DYSEDKSSWDNQQENPPPTKKIGKKPVAKMPLRR PKMKKTPEKLDNTPASPPRSPAEPNDIPIAKGT YTFDIDKWDDPNFNPFSSTSKMQESPKLPQQSY NFDPDTCDESVDPFKTSSKTPSSPSKSPASFEIP ASAMEANGVDGDGLNKPAKKKKTPLKTDTFRVK KSPKRSPLSDPPSQDPTPAATPETPPVISAVVH ATDEEKLAVTNQKWTCMTVDLEADKQDYPQPSDL STFVNETKFSSPTEELDYRNSYEIEYMEKIGSS LPQDDDAPKKQALYLMFDTSQESPVKSSPVRMS ESPTPCSGSSFEETEALVNTAAKNQHPVPRGLAP NQESHLQVPEKSSQKELEAMGLGTPSEAIEITA PEGSFASADALLSRLAHPVSLCGALDYLEPDLA EKNPPLFAQKLQEELEFAIMRIEALKLARQIALA SRSHQDAKREAAHPTDVSISKTALYSRIGTAEV EKPAGLLFQQPDLDSALQIARAEIITKEREVSE WKDKYEESRREVMEMRKIVAEYEKTIAQMIEDEQ REKSVSHQTVQQLVLEKEQALADLNSVEKSLAD LFRRYEKMKEVLEGFRKNEEVLKRCAQEYLSRV KKEEQRYQALKVHAEEKLDRANAEIAQVRGKAQQ EQAAHQASLRKEQLRVDALERTLEQKNKEIEEL TKICDELIAKMGKS*L*TECFGLNCCVQYDRRH TAVPPVPWTGSVFTFSYALLYFLSK*N*FDCMQY *GDYQNFLLLVCIFLV*FIAS*PQSSCIREIV* FSNKRHIADLGLALCTQVPRVSSFWI*YEHVQR A*GLLP*GV*T*SAFADLFLKKQEMHVSNKILYC K*NFFFGSWQ

Frame 1

PAPREGSGEKKRKSQNRANI*NPTLRVVQCTGI SQVTRLVLFQVYFTSFHSSLKLF*KLSLLL*KL SWFLTLPYAPWANFFL*GGKNRVAAADTSDSLLV TAGVRHL*QNSGDAGNTESTWAMRTAPRTTRGL YQLRLQGPRSHPGTVRI*KGSSRTRPEALTTET RPVHLWQMISSSPLPPQTWKAQP*LPLPTTVMLL ARSLRI**PRGVPILKRHLRPRSQRPLSKLRQL HPHHPPKSSQNPRSAHSHPRKNQDVVLRQSLSL MAHGATRWKEVPSVPRHTPSLPSSMKTSR*PAVG LTTWTLTTLSLWIPFRPWSLVPQTLRIRRAK*T HGGSPRIPSPSLSLHCPGRSACKPVTLMVLLPQ AIPRPWPLPQMHIARVPAVLLVPLSELKNRGRLP *KRNRPPRNPQRPPQ*RRRNRSQMKRALSPVGR I*HLRRKRNLPRRKVLAQPYWRRRPLSPLWGPK LPALWTQRVQKGLSPRLLEVAECRTHPLSGGKRC LLPRPRRQGR*PHRIAGGKRTLQPKGSP*GWSL TILRTRVVGTTSRKTPLLPKR*AKSQLPKCP*G GQR*KRHPRNLTTLLPHLPDPLLNPMTSPLLKVL TPLILTSGMTPILTLFLPPQKCRSLPNCPNNHT TLTQTPVMSPLTPLRHPLRPPAHLLNPQPPLRS QPVLWKPMEWTGMG*TSPPRRRRRP*RLTHLG*K SRQNGLLSLIHLPRTPPQLLHQKHHQ*SLRWST PQMRKSWRSPTRSGRA*QWT*RLTNRTTRSPRT CPPL*TRPNSVHPLRSWITETPMKLNIWRKLAPP YLRTTMPRRSRPCTLCLTLLRRALSSHLPSACQ SPRRRVQGQVLKRLKPL*TLLRKTSILSHEDWP LTKSHTCRCQRNPPRRSWRPWAWAPLQKRLKLQL PRAPLPLLTPSSAG*LTPSLSVVHLTIWSPT*Q KRTPHYSLRNSRRS*SLPSCG*KP*SWPGRLLW LPAATRMPRERLLTQQTSPSPKQPCTPASGPLRW RNLQAFCSSSPTWTLPSRSPEQRS*PRRERSQN GKINMKKAGGK*WK*GK*WPSMRRPSLR**RTN RERSQSPTRRCSSWFWRRSKPWPT*TPWRSLWPT SSEDMRR*RRS*KASARMKRC*RDVRRSTCPG* RRRSRGTRP*RCTRRRNWTGPMLRLLRFEARPS RSKPPTRPACGRSSCEWTPWKGRWSRRIKK*KNS PRFVTN*LPKWGKANSEPNVLDLTVACNMTVGT LLFLQFHGQVLFSLFRMHYCISFLNKIDLIVCS TKETIRISCYWFAFS*YNS*QVDLRVPVSGRLSD SLIKDTLLTLALPFVHKFPG*AAFGFNMNMYSV HRDSCLKECKLDLHLLICF*KNKKCMFQIKFSI VNKIFSLDLG

Frame 2

LLQGKDQERRNANPRTVPTYKTPH*GLYSALGF LKSPAWSSSKYTLLPFIPL*NFFKNFHSCSKSY LGFLLYLMPLGRIFSSEEGRIELLLQTHQIPYW *QLECVTSDKILGTLGTLNQHGQ*EQHLGQPEDF ISSDSKVRAATREQSEYKKEAAGHARKP*PQRR VQFTCGR*YHPARCPRRPGKPNLSCLFLPR*CC WPGLYGSDSPEEFRF*RGI*DPGVNDPCQSSASS TPTTPRSHPRTRGQHTATPGRTRMWF*DSPCP* WPTERLGGRKSLPSPVTLLLCRLR*RQADSQQW DLQLGL*QH*ACGYLSDLGASCLRR*ESGGQSEH TEEVHGFRPHL*VYTVPVAQPASQ*L*WCFFLR QSRGRGPCPRCI*HGFQQCF*YP*AN*KTEAAF LKKETDHQETHRDPPSEGDATGAR*REPCPQWGE SSI*DENGICQDGRS*PSLIGGDAP*ARCGAQS CLPSGLRECRRGCPPGFWRWQSAELTPCREENA ASYHGPGGRGGNPIG*RGARGLSSQRALRKAGV* LF*GQE*LGQPAGKPPSYQKDRQKASCQNAPEE AKDEKDTRET*QHSCLTSQIPC*TQ*HPHC*RY LHL*Y*QVG*PQF*PFFFHLKNAGVSQTAPTIIQ L*PRHL**VR*PL*DIL*DPQLTF*IPSLL*DP SQCYGSQWSGRGWAKQARQEEEDAPKD*HI*GE KVAKTVSSL*STFPGPHPSCYTRNTTSDLCGGPR HR*GKAGGHQPEVDVHDSGPRG*QTGLPAALGP VHLCKRDQIQFTH*GVGLQKLL*N*IYGENWLL LTSGRRCPEEAGLVPYV*HFSGEPCQVISRPHVR VPDAVFRVKF*RD*SPCEHCCEKPASCPTRTGP *PRVTLAGAREILPEGAGGHGLGHPFRSD*NYS SRGLLCLC*RPPQQASSPRLSLWCT*LSGARLSR KEPPTIRSETPGGVRVCHHADRSPEAGQADCFG FPQPPGCQERGCSPNRRLHLQNSLVLPHRDR*G GETCRPSVPAARPGLCPPDRQSRDHNQGERGLRM ER*I*RKQAGSDGNEENSGRV*EDHRSDDRGRT EREVSLPPDGAAAGSGEGASPGRPELRGEVSGR PLQKI*EDEGGPRRLPQE*RGVEEMCAGVPVPGE EGGAEVPGPEGARGGETGQGQC*DCSGSRQGPA GASRPPGQPAEGAAASGRPGKDAGAEE*RNRRT HQDL*RTDCQNGEKLTLNRMFWT*LLRAI*PSAH CCSSSSMDRFCFHFFVCTTVFPF*IKLI*LYAV LRRLSEFLAIGLHFPSIIHSKLTSEFLYQGDCL IL**KTHC*PWPCPLYTSSQGEQLLDLI*TCTAC IGTLALRSVNLICIC*FVFKKTRNACFK*NSLL *IKFFLWILA

Sequence NM_006997

CCGGGGGGTTTAAGAGAAGAGCCTTTCGGACCACACCGGCGCTCACGCTCATACCCGCACGCCCCGGGCAGAGCCGCGCACGCCGGCCACACTCGGGCGC GCGCCGGCCACACTCGCGCGCACACATACGCGGCGCTCGCCCCCCGGCCCCCGGCTCGGGCCGCGAGTCGCAGCTCCCTGCCGCCGCTCCCGCCGCCACG GATGCCCGCAGCTGCTCCCCTCTGCAGTGCAGCAACCCCGGCCGCCGGCCGGCTCGCCCCGGCTCCCGGCTGCAGGAATCGCGCCAGGACGCTGGCCCCG CTCGCGGCTAGCTTGCACGCCAGGGCACAGCGAGGATGGGAGGGTCGCAGTCCCTGCAGCCAGCCCCAGCCAGCGACCTGAACCTGGAGGCTTCCGAGGC AATGAGTTCCGATTCTGAAGAGGCATTTGAGACCCCGGAGTCAACGACCCCTGTCAAAGCTCCGCCAGCTCCACCCCCACCACCCCCCGAAGTCATCCCA GAACCCGAGGTCAGCACACAGCCACCCCCGGAAGAACCAGGATGTGGTTCTGAGACAGTCCCTGTCCCTGATGGCCCACGGAGCGACTCGGTGGAAGGAA GTCCCTTCCGTCCCCCGTCACACTCCTTCTCTGCCGTCTTCGATGAAGACAAGCCGATAGCCAGCAGTGGGACTTACAACTTGGACTTTGACAACATTGA GCTTGTGGATACCTTTCAGACCTTGGAGCCTCGTGCCTCAGACGCTAAGAATCAGGAGGGCAAAGTGAACACACGGAGGAAGTCCACGGATTCCGTCCCC ATCTCTAAGTCTACACTGTCCCGGTCGCTCAGCCTGCAAGCCAGTGACTTTGATGGTGCTTCTTCCTCAGGCAATCCCGAGGCCGTGGCCCTTGCCCCAG ATGCATATAGCACGGGTTCCAGCAGTGCTTCTAGTACCCTTAAGCGAACTAAAAAACCGAGGCCGCCTTCCTTAAAAAAGAAACAGACCACCAAGAAACC CACAGAGACCCCCCCAGTGAAGGAGACGCAACAGGAGCCAGATGAAGAGAGCCTTGTCCCCAGTGGGGAGAATCTAGCATCTGAGACGAAAACGGAATCT GCCAAGACGGAAGGTCCTAGCCCAGCCTTATTGGAGGAGACGCCCCTTGAGCCCGCTGTGGGGCCCAAAGCTGCCTGCCCTCTGGACTCAGAGAGTGCAG AAGGGGTTGTCCCCCCGGCTTCTGGAGGTGGCAGAGTGCAGAACTCACCCCCTGTCGGGAGGAAAACGCTGCCTCTTACCACGGCCCCGGAGGCAGGGGA GGTAACCCCATCGGATAGCGGGGGGCAAGAGGACTCTCCAGCCAAAGGGCTCTCCGTAAGGCTGGAGTTTGACTATTCTGAGGACAAGAGTAGTTGGGAC AACCAGCAGGAAAACCCCCCTCCTACCAAAAAGATAGGCAAAAAGCCAGTTGCCAAAATGCCCCTGAGGAGGCCAAAGATGAAAAAGACACCCGAGAAAC TTGACAACACTCCTGCCTCACCTCCCAGATCCCCTGCTGAACCCAATGACATCCCCATTGCTAAAGGTACTTACACCTTTGATATTGACAAGTGGGATGA CCCCAATTTTAACCCTTTTTCTTCCACCTCAAAAATGCAGGAGTCTCCCAAACTGCCCCAACAATCATACAACTTTGACCCAGACACCTGTGATGAGTCC GTTGACCCCTTTAAGACATCCTCTAAGACCCCCAGCTCACCTTCTAAATCCCCAGCCTCCTTTGAGATCCCAGCCAGTGCTATGGAAGCCAATGGAGTGG ACGGGGATGGGCTAAACAAGCCCGCCAAGAAGAAGAAGACGCCCCTAAAGACTGACACATTTAGGGTGAAAAAGTCGCCAAAACGGTCTCCTCTCTCTGA TCCACCTTCCCAGGACCCCACCCCAGCTGCTACACCAGAAACACCACCAGTGATCTCTGCGGTGGTCCACGCCACAGATGAGGAAAAGCTGGCGGTCACC AACCAGAAGTGGACGTGCATGACAGTGGACCTAGAGGCTGACAAACAGGACTACCCGCAGCCCTCGGACCTGTCCACCTTTGTAAACGAGACCAAATTCA GTTCACCCACTGAGGAGTTGGATTACAGAAACTCCTATGAAATTGAATATATGGAGAAAATTGGCTCCTCCTTACCTCAGGACGACGATGCCCCGAAGAA GCAGGCCTTGTACCTTATGTTTGACACTTCTCAGGAGAGCCCTGTCAAGTCATCTCCCGTCCGCATGTCAGAGTCCCCGACGCCGTGTTCAGGGTCAAGT TTTGAAGAGACTGAAGCCCTTGTGAACACTGCTGCGAAAAACCAGCATCCTGTCCCACGAGGACTGGCCCCTAACCAAGAGTCACACTTGCAGGTGCCAG AGAAATCCTCCCAGAAGGAGCTGGAGGCCATGGGCTTGGGCACCCCTTCAGAAGCGATTGAAATTACAGCTCCCGAGGGCTCCTTTGCCTCTGCTGACGC CCTCCTCAGCAGGCTAGCTCACCCCGTCTCTCTCTGTGGTGCACTTGACTATCTGGAGCCCGACTTAGCAGAAAAGAACCCCCCACTATTCGCTCAGAAA CTCCAGGAGGAGTTAGAGTTTGCCATCATGCGGATAGAAGCCCTGAAGCTGGCCAGGCAGATTGCTTTGGCTTCCCGCAGCCACCAGGATGCCAAGAGAG AGGCTGCTCACCCAACAGACGTCTCCATCTCCAAAACAGCCTTGTACTCCCGCATCGGGACCGCTGAGGTGGAGAAACCTGCAGGCCTTCTGTTCCAGCA GCCCGACCTGGACTCTGCCCTCCAGATCGCCAGAGCAGAGATCATAACCAAGGAGAGAGAGGTCTCAGAATGGAAAGATAAATATGAAGAAAGCAGGCGG GAAGTGATGGAAATGAGGAAAATAGTGGCCGAGTATGAGAAGACCATCGCTCAGATGATAGAGGACGAACAGAGAGAGAAGTCAGTCTCCCACCAGACGG TGCAGCAGCTGGTTCTGGAGAAGGAGCAAGCCCTGGCCGACCTGAACTCCGTGGAGAAGTCTCTGGCCGACCTCTTCAGAAGATATGAGAAGATGAAGGA GGTCCTAGAAGGCTTCCGCAAGAATGAAGAGGTGTTGAAGAGATGTGCGCAGGAGTACCTGTCCCGGGTGAAGAAGGAGGAGCAGAGGTACCAGGCCCTG AAGGTGCACGCGGAGGAGAAACTGGACAGGGCCAATGCTGAGATTGCTCAGGTTCGAGGCAAGGCCCAGCAGGAGCAAGCCGCCCACCAGGCCAGCCTGC GGAAGGAGCAGCTGCGAGTGGACGCCCTGGAAAGGACGCTGGAGCAGAAGAATAAAGAAATAGAAGAACTCACCAAGATTTGTGACGAACTGATTGCCAA AATGGGGAAAAGCTAACTCTGAACCGAATGTTTTGGACTTAACTGTTGCGTGCAATATGACCGTCGGCACACTGCTGTTCCTCCAGTTCCATGGACAGGT TCTGTTTTCACTTTTTCGTATGCACTACTGTATTTCCTTTCTAAATAAAATTGATTTGATTGTATGCAGTACTAAGGAGACTATCAGAATTTCTTGCTAT TGGTTTGCATTTTCCTAGTATAATTCATAGCAAGTTGACCTCAGAGTTCCTGTATCAGGGAGATTGTCTGATTCTCTAATAAAAGACACATTGCTGACCT TGGCCTTGCCCTTTGTACACAAGTTCCCAGGGTGAGCAGCTTTTGGATTTAATATGAACATGTACAGCGTGCATAGGGACTCTTGCCTTAAGGAGTGTAA ACTTGATCTGCATTTGCTGATTTGTTTTTAAAAAAACAAGAAATGCATGTTTCAAATAAAATTCTCTATTGTAAATAAAATTTTTTCTTTGGATCTTGGC AA

refseq_TACC2.F1 (forward primer)
refseq_TACC2.R1 (reverse primer)
Single exon skipping

Frame 0

PGGLREEPFGPHRRSRSYPHAPGRAAHAGHTRA RAGHTRAHTYAALAPRPPARAASRSSLPPLPPPR MPAAAPLCSAATPAAGRLAPAPGCRNRARTLAP LAASLHARAQRGWEGRSPCSQPQPAT*TWRLPR Q*VPILKRHLRPRSQRPLSKLRQLHPHHPPKSSQ NPRSAHSHPRKNQDVVLRQSLSLMAHGATRWKE VPSVPRHTPSLPSSMKTSR*PAVGLTTWTLTTL SLWIPFRPWSLVPQTLRIRRAK*THGGSPRIPSP SLSLHCPGRSACKPVTLMVLLPQAIPRPWPLPQ MHIARVPAVLLVPLSELKNRGRLP*KRNRPPRN PQRPPQ*RRRNRSQMKRALSPVGRI*HLRRKRNL PRRKVLAQPYWRRRPLSPLWGPKLPALWTQRVQ KGLSPRLLEVAECRTHPLSGGKRCLLPRPRRQG R*PHRIAGGKRTLQPKGSP*GWSLTILRTRVVGT TSRKTPLLPKR*AKSQLPKCP*GGQR*KRHPRN LTTLLPHLPDPLLNPMTSPLLKVLTPLILTSGM TPILTLFLPPQKCRSLPNCPNNHTTLTQTPVMSP LTPLRHPLRPPAHLLNPQPPLRSQPVLWKPMEW TGMG*TSPPRRRRRP*RLTHLG*KSRQNGLLSL IHLPRTPPQLLHQKHHQ*SLRWSTPQMRKSWRSP TRSGRA*QWT*RLTNRTTRSPRTCPPL*TRPNS VHPLRSWITETPMKLNIWRKLAPPYLRTTMPRR SRPCTLCLTLLRRALSSHLPSACQSPRRRVQGQV LKRLKPL*TLLRKTSILSHEDWPLTKSHTCRCQ RNPPRRSWRPWAWAPLQKRLKLQLPRAPLPLLT PSSAG*LTPSLSVVHLTIWSPT*QKRTPHYSLRN SRRS*SLPSCG*KP*SWPGRLLWLPAATRMPRE RLLTQQTSPSPKQPCTPASGPLRWRNLQAFCSS SPTWTLPSRSPEQRS*PRRERSQNGKINMKKAGG K*WK*GK*WPSMRRPSLR**RTNRERSQSPTRR CSSWFWRRSKPWPT*TPWRSLWPTSSEDMRR*R RS*KASARMKRC*RDVRRSTCPG*RRRSRGTRP* RCTRRRNWTGPMLRLLRFEARPSRSKPPTRPAC GRSSCEWTPWKGRWSRRIKK*KNSPRFVTN*LP KWGKANSEPNVLDLTVACNMTVGTLLFLQFHGQV LFSLFRMHYCISFLNKIDLIVCSTKETIRISCY WFAFS*YNS*QVDLRVPVSGRLSDSLIKDTLLT LALPFVHKFPG*AAFGFNMNMYSVHRDSCLKECK LDLHLLICF*KNKKCMFQIKFSIVNKIFSLDLG

Frame 1

RGV*EKSLSDHTGAHAHTRTPRAEPRTPATLGR APATLARTHTRRSPPGPRLGPRVAAPCRRSRRH GCPQLLPSAVQQPRPPAGSPRLPAAGIAPGRWPR SRLACTPGHSEDGRVAVPAASPSQRPEPGGFRG NEFRF*RGI*DPGVNDPCQSSASSTPTTPRSHP RTRGQHTATPGRTRMWF*DSPCP*WPTERLGGRK SLPSPVTLLLCRLR*RQADSQQWDLQLGL*QH* ACGYLSDLGASCLRR*ESGGQSEHTEEVHGFRP HL*VYTVPVAQPASQ*L*WCFFLRQSRGRGPCPR CI*HGFQQCF*YP*AN*KTEAAFLKKETDHQET HRDPPSEGDATGAR*REPCPQWGESSI*DENGI CQDGRS*PSLIGGDAP*ARCGAQSCLPSGLRECR RGCPPGFWRWQSAELTPCREENAASYHGPGGRG GNPIG*RGARGLSSQRALRKAGV*LF*GQE*LG QPAGKPPSYQKDRQKASCQNAPEEAKDEKDTRET *QHSCLTSQIPC*TQ*HPHC*RYLHL*Y*QVG* PQF*PFFFHLKNAGVSQTAPTIIQL*PRHL**V R*PL*DIL*DPQLTF*IPSLL*DPSQCYGSQWSG RGWAKQARQEEEDAPKD*HI*GEKVAKTVSSL* STFPGPHPSCYTRNTTSDLCGGPRHR*GKAGGH QPEVDVHDSGPRG*QTGLPAALGPVHLCKRDQIQ FTH*GVGLQKLL*N*IYGENWLLLTSGRRCPEE AGLVPYV*HFSGEPCQVISRPHVRVPDAVFRVK F*RD*SPCEHCCEKPASCPTRTGP*PRVTLAGAR EILPEGAGGHGLGHPFRSD*NYSSRGLLCLC*R PPQQASSPRLSLWCT*LSGARLSRKEPPTIRSE TPGGVRVCHHADRSPEAGQADCFGFPQPPGCQER GCSPNRRLHLQNSLVLPHRDR*GGETCRPSVPA ARPGLCPPDRQSRDHNQGERGLRMER*I*RKQA GSDGNEENSGRV*EDHRSDDRGRTEREVSLPPDG AAAGSGEGASPGRPELRGEVSGRPLQKI*EDEG GPRRLPQE*RGVEEMCAGVPVPGEEGGAEVPGP EGARGGETGQGQC*DCSGSRQGPAGASRPPGQPA EGAAASGRPGKDAGAEE*RNRRTHQDL*RTDCQ NGEKLTLNRMFWT*LLRAI*PSAHCCSSSSMDR FCFHFFVCTTVFPF*IKLI*LYAVLRRLSEFLAI GLHFPSIIHSKLTSEFLYQGDCLIL**KTHC*P WPCPLYTSSQGEQLLDLI*TCTACIGTLALRSV NLICIC*FVFKKTRNACFK*NSLL*IKFFLWILA

Frame 2

GGFKRRAFRTTPALTLIPARPGQSRARRPHSGA RRPHSRAHIRGARPPAPGSGRESQLPAAAPAAT DARSCSPLQCSNPGRRPARPGSRLQESRQDAGP ARG*LARQGTARMGGSQSLQPAPASDLNLEASEA MSSDSEEAFETPESTTPVKAPPAPPPPPPEVIP EPEVSTQPPPEEPGCGSETVPVPDGPRSDSVEG SPFRPPSHSFSAVFDEDKPIASSGTYNLDFDNIE LVDTFQTLEPRASDAKNQEGKVNTRRKSTDSVP ISKSTLSRSLSLQASDFDGASSSGNPEAVALAP DAYSTGSSSASSTLKRTKKPRPPSLKKKQTTKKP TETPPVKETQQEPDEESLVPSGENLASETKTES AKTEGPSPALLEETPLEPAVGPKAACPLDSESA EGVVPPASGGGRVQNSPPVGRKTLPLTTAPEAGE VTPSDSGGQEDSPAKGLSVRLEFDYSEDKSSWD NQQENPPPTKKIGKKPVAKMPLRRPKMKKTPEK LDNTPASPPRSPAEPNDIPIAKGTYTFDIDKWDD PNFNPFSSTSKMQESPKLPQQSYNFDPDTCDES VDPFKTSSKTPSSPSKSPASFEIPASAMEANGV DGDGLNKPAKKKKTPLKTDTFRVKKSPKRSPLSD PPSQDPTPAATPETPPVISAVVHATDEEKLAVT NQKWTCMTVDLEADKQDYPQPSDLSTFVNETKF SSPTEELDYRNSYEIEYMEKIGSSLPQDDDAPKK QALYLMFDTSQESPVKSSPVRMSESPTPCSGSS FEETEALVNTAAKNQHPVPRGLAPNQESHLQVP EKSSQKELEAMGLGTPSEAIEITAPEGSFASADA LLSRLAHPVSLCGALDYLEPDLAEKNPPLFAQK LQEELEFAIMRIEALKLARQIALASRSHQDAKR EAAHPTDVSISKTALYSRIGTAEVEKPAGLLFQQ PDLDSALQIARAEIITKEREVSEWKDKYEESRR EVMEMRKIVAEYEKTIAQMIEDEQREKSVSHQT VQQLVLEKEQALADLNSVEKSLADLFRRYEKMKE VLEGFRKNEEVLKRCAQEYLSRVKKEEQRYQAL KVHAEEKLDRANAEIAQVRGKAQQEQAAHQASL RKEQLRVDALERTLEQKNKEIEELTKICDELIAK MGKS*L*TECFGLNCCVQYDRRHTAVPPVPWTG SVFTFSYALLYFLSK*N*FDCMQY*GDYQNFLL LVCIFLV*FIAS*PQSSCIREIV*FSNKRHIADL GLALCTQVPRVSSFWI*YEHVQRA*GLLP*GV* T*SAFADLFLKKQEMHVSNKILYCK*NFFFGSW Q