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Sequences

Sequence NM_175721

AAGGCAATTAAGGCGCCCATTTCAGAAGAGTTACAGCCGTGAAAATTACTCAGCAGTGCAGTTGGCTGAGAAGAGGAAAAAAGAATGAGAGCGCTCGCTG TGCTGTCTGTCACGCTGGTTATGGCCTGCACAGAAGCCTTCTTCCCCTTCATCTCGAGAGGGAAAGAACTCCTTTGGGGAAAGCCTGAGGAGTCTCGTGT CTCTAGCGTCTTGGAGGAAAGCAAGCGCCTGGTGGACACCGCCATGTACGCCACGATGCAGAGAAACCTCAAGAAAAGAGGAATCCTTTCTCCAGCTCAG CTTCTGTCTTTTTCCAAACTTCCTGAGCCAACAAGCGGAGTGATTGCCCGAGCAGCAGAGATAATGGAAACATCAATACAAGCGATGAAAAGAAAAGTCA ACCTGAAAACTCAACAATCACAGCATCCAACGGATGCTTTATCAGAAGATCTGCTGAGCATCATTGCAAACATGTCTGGATGTCTCCCTTACATGCTGCC CCCAAAATGCCCAAACACTTGCCTGGCGAACAAATACAGGCCCATCACAGGAGCTTGCAACAACAGAGACCACCCCAGATGGGGCGCCTCCAACACGGCC CTGGCACGATGGCTCCCTCCAGTCTATGAGGACGGCTTCAGTCAGCCCCGAGGCTGGAACCCCGGCTTCTTGTACAACGGGTTCCCACTGCCCCCGGTCC GGGAGGTGACAAGACATGTCATTCAAGTTTCAAATGAGGTTGTCACAGATGATGACCGCTATTCTGACCTCCTGATGGCATGGGGACAATACATCGACCA CGACATCGCGTTCACACCACAGAGCACCAGCAAAGCTGCCTTCGGGGGAGGGGCTGACTGCCAGATGACTTGTGAGAACCAAAACCCATGTTTTCCCATA CAACTCCCGGAGGAGGCCCGGCCGGCCGCGGGCACCGCCTGTCTGCCCTTCTACCGCTCTTCGGCCGCCTGCGGCACCGGGGACCAAGGCGCGCTCTTTG GGAACCTGTCCACGGCCAACCCGCGGCAGCAGATGAACGGGTTGACCTCGTTCCTGGACGCGTCCACCGTGTATGGCAGCTCCCCGGCCCTAGAGAGGCA GCTGCGGAACTGGACCAGTGCCGAAGGGCTGCTCCGCGTCCACGCGCGCCTCCGGGACTCCGGCCGCGCCTACCTGCCCTTCGTGCCGCCACGCGCGCCT GCGGCCTGTGCGCCCGAGCCCGGCATCCCCGGAGAGACCCGCGGGCCCTGCTTCCTGGCCGGAGACGGCCGCGCCAGCGAGGTCCCCTCCCTGACGGCAC TGCACACGCTGTGGCTGCGCGAGCACAACCGCCTGGCCGCGGCGCTCAAGGCCCTCAATGCGCACTGGAGCGCGGACGCCGTGTACCAGGAGGCGCGCAA GGTCGTGGGCGCTCTGCACCAGATCATCACCCTGAGGGATTACATCCCCAGGATCCTGGGACCCGAGGCCTTCCAGCAGTACGTGGGTCCCTATGAAGGC TATGACTCCACCGCCAACCCCACTGTGTCCAACGTGTTCTCCACAGCCGCCTTCCGCTTCGGCCATGCCACGATCCACCCGCTGGTGAGGAGGCTGGACG CCAGCTTCCAGGAGCACCCCGACCTGCCCGGGCTGTGGCTGCACCAGGCTTTCTTCAGCCCATGGACATTACTCCGTGGAGGTGGTTTGGACCCACTAAT ACGAGGCCTTCTTGCAAGACCAGCCAAACTGCAGGTGCAGGATCAGCTGATGAACGAGGAGCTGACGGAAAGGCTCTTTGTGCTGTCCAATTCCAGCACC TTGGATCTGGCGTCCATCAACCTGCAGAGGGGCCGGGACCACGGGCTGCCAGGTTACAATGAGTGGAGGGAGTTCTGCGGCCTGCCTCGCCTGGAGACCC CCGCTGACCTGAGCACAGCCATCGCCAGCAGGAGCGTGGCCGACAAGATCCTGGACTTGTACAAGCATCCTGACAACATCGATGTCTGGCTGGGAGGCTT AGCTGAAAACTTCCTCCCCAGGGCTCGGACAGGGCCCCTGTTTGCCTGTCTCATTGGGAAGCAGATGAAGGCTCTGCGGGACGGTGACTGGTTTTGGTGG GAGAACAGCCACGTCTTCACGGATGCACAGAGGCGTGAGCTGGAGAAGCACTCCCTGTCTCGGGTCATCTGTGACAACACTGGCCTCACCAGGGTGCCCA TGGATGCCTTCCAAGTCGGCAAATTCCCCGAAGACTTTGAGTCTTGTGACAGCATCACTGGCATGAACCTGGAGGCCTGGAGGGAAACCTTTCCTCAAGA CGACAAGTGTGGCTTCCCAGAGAGCGTGGAGAATGGGGACTTTGTGCACTGTGAGGAGTCTGGGAGGCGCGTGCTGGTGTATTCCTGCCGGCACGGGTAT GAGCTCCAAGGCCGGGAGCAGCTCACTTGCACCCAGGAAGGATGGGATTTCCAGCCTCCCCTCTGCAAAGACTCCGGGAGGCTCCCTCGGGTGACTTGGA TCTCCATGTCGCTGGCTGCTCTGCTGATCGGAGGCTTCGCAGGTCTCACCTCGACGGTGATTTGCAGGTGGACACGCACTGGCACTAAATCCACACTGCC CATCTCGGAGACAGGCGGAGGAACTCCCGAGCTGAGATGCGGAAAGCACCAGGCCGTAGGGACCTCACCGCAGCGGGCCGCAGCTCAGGACTCGGAGCAG GAGAGTGCTGGGATGGAAGGCCGGGATACTCACAGGCTGCCGAGAGCCCTCTGAGGGCAAAGTGGCAGGACACTGCAGAACAGCTTCATGTTCCCAAAAT CACCGTACGACTCTTTTCCAAACACAGGCAAATCCGAAATCAGCAGGACGACTGTTTTCCCAACACGGGTAAATCTAGTACCATGTCGTAGTTACTCTCA GGCATGGATGAATAAATGTTATAGCTGCATTTGTCTGGCCTTTTCTTGTAAACATTGCCTGATTTGTTCCTTCTGGGGCTTTGCCATTAAAATGTATTTA CAG

refseq_TPO.F4 (forward primer)
refseq_TPO.R4 (reverse primer)
Single exon skipping

Frame 0

KAIKAPISEELQP*KLLSSAVG*EEEKRMRALA VLSVTLVMACTEAFFPFISRGKELLWGKPEESRV SSVLEESKRLVDTAMYATMQRNLKKRGILSPAQ LLSFSKLPEPTSGVIARAAEIMETSIQAMKRKV NLKTQQSQHPTDALSEDLLSIIANMSGCLPYMLP PKCPNTCLANKYRPITGACNNRDHPRWGASNTA LARWLPPVYEDGFSQPRGWNPGFLYNGFPLPPV REVTRHVIQVSNEVVTDDDRYSDLLMAWGQYIDH DIAFTPQSTSKAAFGGGADCQMTCENQNPCFPI QLPEEARPAAGTACLPFYRSSAACGTGDQGALF GNLSTANPRQQMNGLTSFLDASTVYGSSPALERQ LRNWTSAEGLLRVHARLRDSGRAYLPFVPPRAP AACAPEPGIPGETRGPCFLAGDGRASEVPSLTA LHTLWLREHNRLAAALKALNAHWSADAVYQEARK VVGALHQIITLRDYIPRILGPEAFQQYVGPYEG YDSTANPTVSNVFSTAAFRFGHATIHPLVRRLD ASFQEHPDLPGLWLHQAFFSPWTLLRGGGLDPLI RGLLARPAKLQVQDQLMNEELTERLFVLSNSST LDLASINLQRGRDHGLPGYNEWREFCGLPRLET PADLSTAIASRSVADKILDLYKHPDNIDVWLGGL AENFLPRARTGPLFACLIGKQMKALRDGDWFWW ENSHVFTDAQRRELEKHSLSRVICDNTGLTRVP MDAFQVGKFPEDFESCDSITGMNLEAWRETFPQD DKCGFPESVENGDFVHCEESGRRVLVYSCRHGY ELQGREQLTCTQEGWDFQPPLCKDSGRLPRVTW ISMSLAALLIGGFAGLTSTVICRWTRTGTKSTLP ISETGGGTPELRCGKHQAVGTSPQRAAAQDSEQ ESAGMEGRDTHRLPRAL*GQSGRTLQNSFMFPK SPYDSFPNTGKSEISRTTVFPTRVNLVPCRSYSQ AWMNKCYSCICLAFSCKHCLICSFWGFAIKMYL Q

Frame 1

RQLRRPFQKSYSRENYSAVQLAEKRKKE*ERSL CCLSRWLWPAQKPSSPSSREGKNSFGESLRSLV SLASWRKASAWWTPPCTPRCRETSRKEESFLQLS FCLFPNFLSQQAE*LPEQQR*WKHQYKR*KEKS T*KLNNHSIQRMLYQKIC*ASLQTCLDVSLTCC PQNAQTLAWRTNTGPSQELATTETTPDGAPPTRP WHDGSLQSMRTASVSPEAGTPASCTTGSHCPRS GR*QDMSFKFQMRLSQMMTAILTS*WHGDNTST TTSRSHHRAPAKLPSGEGLTAR*LVRTKTHVFPY NSRRRPGRPRAPPVCPSTALRPPAAPGTKARSL GTCPRPTRGSR*TG*PRSWTRPPCMAAPRP*RG SCGTGPVPKGCSASTRASGTPAAPTCPSCRHARL RPVRPSPASPERPAGPASWPETAAPARSPP*RH CTRCGCASTTAWPRRSRPSMRTGARTPCTRRRA RSWALCTRSSP*GITSPGSWDPRPSSSTWVPMKA MTPPPTPLCPTCSPQPPSASAMPRSTRW*GGWT PASRSTPTCPGCGCTRLSSAHGHYSVEVVWTH* YEAFLQDQPNCRCRIS**TRS*RKGSLCCPIPAP WIWRPSTCRGAGTTGCQVTMSGGSSAACLAWRP PLT*AQPSPAGAWPTRSWTCTSILTTSMSGWEA *LKTSSPGLGQGPCLPVSLGSR*RLCGTVTGFGG RTATSSRMHRGVSWRSTPCLGSSVTTLASPGCP WMPSKSANSPKTLSLVTASLA*TWRPGGKPFLK TTSVASQRAWRMGTLCTVRSLGGACWCIPAGTGM SSKAGSSSLAPRKDGISSLPSAKTPGGSLG*LG SPCRWLLC*SEASQVSPRR*FAGGHALALNPHC PSRRQAEELPS*DAESTRP*GPHRSGPQLRTRSR RVLGWKAGILTGCREPSEGKVAGHCRTASCSQN HRTTLFQTQANPKSAGRLFSQHG*I*YHVVVTL RHG*INVIAAFVWPFLVNIA*FVPSGALPLKCIY

Frame 2

GN*GAHFRRVTAVKITQQCSWLRRGKKNESARC AVCHAGYGLHRSLLPLHLERERTPLGKA*GVSC L*RLGGKQAPGGHRHVRHDAEKPQEKRNPFSSS ASVFFQTS*ANKRSDCPSSRDNGNINTSDEKKSQ PENSTITASNGCFIRRSAEHHCKHVWMSPLHAA PKMPKHLPGEQIQAHHRSLQQQRPPQMGRLQHG PGTMAPSSL*GRLQSAPRLEPRLLVQRVPTAPGP GGDKTCHSSFK*GCHR**PLF*PPDGMGTIHRP RHRVHTTEHQQSCLRGRG*LPDDL*EPKPMFSH TTPGGGPAGRGHRLSALLPLFGRLRHRGPRRALW EPVHGQPAAADERVDLVPGRVHRVWQLPGPREA AAELDQCRRAAPRPRAPPGLRPRLPALRAATRA CGLCARARHPRRDPRALLPGRRRPRQRGPLPDGT AHAVAARAQPPGRGAQGPQCALERGRRVPGGAQ GRGRSAPDHHPEGLHPQDPGTRGLPAVRGSL*R L*LHRQPHCVQRVLHSRLPLRPCHDPPAGEEAGR QLPGAPRPARAVAAPGFLQPMDITPWRWFGPTN TRPSCKTSQTAGAGSADERGADGKALCAVQFQH LGSGVHQPAEGPGPRAARLQ*VEGVLRPASPGDP R*PEHSHRQQERGRQDPGLVQAS*QHRCLAGRL S*KLPPQGSDRAPVCLSHWEADEGSAGR*LVLV GEQPRLHGCTEA*AGEALPVSGHL*QHWPHQGAH GCLPSRQIPRRL*VL*QHHWHEPGGLEGNLSSR RQVWLPRERGEWGLCAL*GVWEARAGVFLPARV *APRPGAAHLHPGRMGFPASPLQRLREAPSGDLD LHVAGCSADRRLRRSHLDGDLQVDTHWH*IHTA HLGDRRRNSRAEMRKAPGRRDLTAAGRSSGLGA GECWDGRPGYSQAAESPLRAKWQDTAEQLHVPKI TVRLFSKHRQIRNQQDDCFPNTGKSSTMS*LLS GMDE*ML*LHLSGLFL*TLPDLFLLGLCH*NVF T

Sequence NM_175722

AAGGCAATTAAGGCGCCCATTTCAGAAGAGTTACAGCCGTGAAAATTACTCAGCAGTGCAGTTGGCTGAGAAGAGGAAAAAAGAATGAGAGCGCTCGCTG TGCTGTCTGTCACGCTGGTTATGGCCTGCACAGAAGCCTTCTTCCCCTTCATCTCGAGAGGGAAAGAACTCCTTTGGGGAAAGCCTGAGGAGTCTCGTGT CTCTAGCGTCTTGGAGGAAAGCAAGCGCCTGGTGGACACCGCCATGTACGCCACGATGCAGAGAAACCTCAAGAAAAGAGGAATCCTTTCTCCAGCTCAG CTTCTGTCTTTTTCCAAACTTCCTGAGCCAACAAGCGGAGTGATTGCCCGAGCAGCAGAGATAATGGAAACATCAATACAAGCGATGAAAAGAAAAGTCA ACCTGAAAACTCAACAATCACAGCATCCAACGGATGCTTTATCAGAAGATCTGCTGAGCATCATTGCAAACATGTCTGGATGTCTCCCTTACATGCTGCC CCCAAAATGCCCAAACACTTGCCTGGCGAACAAATACAGGCCCATCACAGGAGCTTGCAACAACAGAGACCACCCCAGATGGGGCGCCTCCAACACGGCC CTGGCACGATGGCTCCCTCCAGTCTATGAGGACGGCTTCAGTCAGCCCCGAGGCTGGAACCCCGGCTTCTTGTACAACGGGTTCCCACTGCCCCCGGTCC GGGAGGTGACAAGACATGTCATTCAAGTTTCAAATGAGGTTGTCACAGATGATGACCGCTATTCTGACCTCCTGATGGCATGGGGACAATACATCGACCA CGACATCGCGTTCACACCACAGAGCACCAGCAAAGCTGCCTTCGGGGGAGGGGCTGACTGCCAGATGACTTGTGAGAACCAAAACCCATGTTTTCCCATA CAAATCATCACCCTGAGGGATTACATCCCCAGGATCCTGGGACCCGAGGCCTTCCAGCAGTACGTGGGTCCCTATGAAGGCTATGACTCCACCGCCAACC CCACTGTGTCCAACGTGTTCTCCACAGCCGCCTTCCGCTTCGGCCATGCCACGATCCACCCGCTGGTGAGGAGGCTGGACGCCAGCTTCCAGGAGCACCC CGACCTGCCCGGGCTGTGGCTGCACCAGGCTTTCTTCAGCCCATGGACATTACTCCGTGGAGGTGGTTTGGACCCACTAATACGAGGCCTTCTTGCAAGA CCAGCCAAACTGCAGGTGCAGGATCAGCTGATGAACGAGGAGCTGACGGAAAGGCTCTTTGTGCTGTCCAATTCCAGCACCTTGGATCTGGCGTCCATCA ACCTGCAGAGGGGCCGGGACCACGGGCTGCCAGGTTACAATGAGTGGAGGGAGTTCTGCGGCCTGCCTCGCCTGGAGACCCCCGCTGACCTGAGCACAGC CATCGCCAGCAGGAGCGTGGCCGACAAGATCCTGGACTTGTACAAGCATCCTGACAACATCGATGTCTGGCTGGGAGGCTTAGCTGAAAACTTCCTCCCC AGGGCTCGGACAGGGCCCCTGTTTGCCTGTCTCATTGGGAAGCAGATGAAGGCTCTGCGGGACGGTGACTGGTTTTGGTGGGAGAACAGCCACGTCTTCA CGGATGCACAGAGGCGTGAGCTGGAGAAGCACTCCCTGTCTCGGGTCATCTGTGACAACACTGGCCTCACCAGGGTGCCCATGGATGCCTTCCAAGTCGG CAAATTCCCCGAAGACTTTGAGTCTTGTGACAGCATCACTGGCATGAACCTGGAGGCCTGGAGGGAAACCTTTCCTCAAGACGACAAGTGTGGCTTCCCA GAGAGCGTGGAGAATGGGGACTTTGTGCACTGTGAGGAGTCTGGGAGGCGCGTGCTGGTGTATTCCTGCCGGCACGGGTATGAGCTCCAAGGCCGGGAGC AGCTCACTTGCACCCAGGAAGGATGGGATTTCCAGCCTCCCCTCTGCAAAGATGTGAACGAGTGTGCAGACGGTGCCCACCCCCCCTGCCACGCCTCTGC GAGGTGCAGAAACACCAAAGGCGGCTTCCAGTGTCTCTGCGCGGACCCCTACGAGTTAGGAGACGATGGGAGAACCTGCGTAGACTCCGGGAGGCTCCCT CGGGTGACTTGGATCTCCATGTCGCTGGCTGCTCTGCTGATCGGAGGCTTCGCAGGTCTCACCTCGACGGTGATTTGCAGGTGGACACGCACTGGCACTA AATCCACACTGCCCATCTCGGAGACAGGCGGAGGAACTCCCGAGCTGAGATGCGGAAAGCACCAGGCCGTAGGGACCTCACCGCAGCGGGCCGCAGCTCA GGACTCGGAGCAGGAGAGTGCTGGGATGGAAGGCCGGGATACTCACAGGCTGCCGAGAGCCCTCTGAGGGCAAAGTGGCAGGACACTGCAGAACAGCTTC ATGTTCCCAAAATCACCGTACGACTCTTTTCCAAACACAGGCAAATCCGAAATCAGCAGGACGACTGTTTTCCCAACACGGGTAAATCTAGTACCATGTC GTAGTTACTCTCAGGCATGGATGAATAAATGTTATAGCTGCATTTGTCTGGCCTTTTCTTGTAAACATTGCCTGATTTGTTCCTTCTGGGGCTTTGCCAT TAAAATGTATTTACAG

refseq_TPO.F4 (forward primer)
refseq_TPO.R4 (reverse primer)
Single exon skipping

Frame 0

KAIKAPISEELQP*KLLSSAVG*EEEKRMRALA VLSVTLVMACTEAFFPFISRGKELLWGKPEESRV SSVLEESKRLVDTAMYATMQRNLKKRGILSPAQ LLSFSKLPEPTSGVIARAAEIMETSIQAMKRKV NLKTQQSQHPTDALSEDLLSIIANMSGCLPYMLP PKCPNTCLANKYRPITGACNNRDHPRWGASNTA LARWLPPVYEDGFSQPRGWNPGFLYNGFPLPPV REVTRHVIQVSNEVVTDDDRYSDLLMAWGQYIDH DIAFTPQSTSKAAFGGGADCQMTCENQNPCFPI QIITLRDYIPRILGPEAFQQYVGPYEGYDSTAN PTVSNVFSTAAFRFGHATIHPLVRRLDASFQEHP DLPGLWLHQAFFSPWTLLRGGGLDPLIRGLLAR PAKLQVQDQLMNEELTERLFVLSNSSTLDLASI NLQRGRDHGLPGYNEWREFCGLPRLETPADLSTA IASRSVADKILDLYKHPDNIDVWLGGLAENFLP RARTGPLFACLIGKQMKALRDGDWFWWENSHVF TDAQRRELEKHSLSRVICDNTGLTRVPMDAFQVG KFPEDFESCDSITGMNLEAWRETFPQDDKCGFP ESVENGDFVHCEESGRRVLVYSCRHGYELQGRE QLTCTQEGWDFQPPLCKDVNECADGAHPPCHASA RCRNTKGGFQCLCADPYELGDDGRTCVDSGRLP RVTWISMSLAALLIGGFAGLTSTVICRWTRTGT KSTLPISETGGGTPELRCGKHQAVGTSPQRAAAQ DSEQESAGMEGRDTHRLPRAL*GQSGRTLQNSF MFPKSPYDSFPNTGKSEISRTTVFPTRVNLVPC RSYSQAWMNKCYSCICLAFSCKHCLICSFWGFAI KMYLQ

Frame 1

RQLRRPFQKSYSRENYSAVQLAEKRKKE*ERSL CCLSRWLWPAQKPSSPSSREGKNSFGESLRSLV SLASWRKASAWWTPPCTPRCRETSRKEESFLQLS FCLFPNFLSQQAE*LPEQQR*WKHQYKR*KEKS T*KLNNHSIQRMLYQKIC*ASLQTCLDVSLTCC PQNAQTLAWRTNTGPSQELATTETTPDGAPPTRP WHDGSLQSMRTASVSPEAGTPASCTTGSHCPRS GR*QDMSFKFQMRLSQMMTAILTS*WHGDNTST TTSRSHHRAPAKLPSGEGLTAR*LVRTKTHVFPY KSSP*GITSPGSWDPRPSSSTWVPMKAMTPPPT PLCPTCSPQPPSASAMPRSTRW*GGWTPASRST PTCPGCGCTRLSSAHGHYSVEVVWTH*YEAFLQD QPNCRCRIS**TRS*RKGSLCCPIPAPWIWRPS TCRGAGTTGCQVTMSGGSSAACLAWRPPLT*AQ PSPAGAWPTRSWTCTSILTTSMSGWEA*LKTSSP GLGQGPCLPVSLGSR*RLCGTVTGFGGRTATSS RMHRGVSWRSTPCLGSSVTTLASPGCPWMPSKS ANSPKTLSLVTASLA*TWRPGGKPFLKTTSVASQ RAWRMGTLCTVRSLGGACWCIPAGTGMSSKAGS SSLAPRKDGISSLPSAKM*TSVQTVPTPPATPL RGAETPKAASSVSARTPTS*ETMGEPA*TPGGSL G*LGSPCRWLLC*SEASQVSPRR*FAGGHALAL NPHCPSRRQAEELPS*DAESTRP*GPHRSGPQL RTRSRRVLGWKAGILTGCREPSEGKVAGHCRTAS CSQNHRTTLFQTQANPKSAGRLFSQHG*I*YHV VVTLRHG*INVIAAFVWPFLVNIA*FVPSGALP LKCIY

Frame 2

GN*GAHFRRVTAVKITQQCSWLRRGKKNESARC AVCHAGYGLHRSLLPLHLERERTPLGKA*GVSC L*RLGGKQAPGGHRHVRHDAEKPQEKRNPFSSS ASVFFQTS*ANKRSDCPSSRDNGNINTSDEKKSQ PENSTITASNGCFIRRSAEHHCKHVWMSPLHAA PKMPKHLPGEQIQAHHRSLQQQRPPQMGRLQHG PGTMAPSSL*GRLQSAPRLEPRLLVQRVPTAPGP GGDKTCHSSFK*GCHR**PLF*PPDGMGTIHRP RHRVHTTEHQQSCLRGRG*LPDDL*EPKPMFSH TNHHPEGLHPQDPGTRGLPAVRGSL*RL*LHRQP HCVQRVLHSRLPLRPCHDPPAGEEAGRQLPGAP RPARAVAAPGFLQPMDITPWRWFGPTNTRPSCK TSQTAGAGSADERGADGKALCAVQFQHLGSGVHQ PAEGPGPRAARLQ*VEGVLRPASPGDPR*PEHS HRQQERGRQDPGLVQAS*QHRCLAGRLS*KLPP QGSDRAPVCLSHWEADEGSAGR*LVLVGEQPRLH GCTEA*AGEALPVSGHL*QHWPHQGAHGCLPSR QIPRRL*VL*QHHWHEPGGLEGNLSSRRQVWLP RERGEWGLCAL*GVWEARAGVFLPARV*APRPGA AHLHPGRMGFPASPLQRCERVCRRCPPPLPRLC EVQKHQRRLPVSLRGPLRVRRRWENLRRLREAP SGDLDLHVAGCSADRRLRRSHLDGDLQVDTHWH* IHTAHLGDRRRNSRAEMRKAPGRRDLTAAGRSS GLGAGECWDGRPGYSQAAESPLRAKWQDTAEQL HVPKITVRLFSKHRQIRNQQDDCFPNTGKSSTMS *LLSGMDE*ML*LHLSGLFL*TLPDLFLLGLCH *NVFT

Sequence NM_175719

AAGGCAATTAAGGCGCCCATTTCAGAAGAGTTACAGCCGTGAAAATTACTCAGCAGTGCAGTTGGCTGAGAAGAGGAAAAAAGGTCAGAATGAGAGCGCT CGCTGTGCTGTCTGTCACGCTGGTTATGGCCTGCACAGAAGCCTTCTTCCCCTTCATCTCGAGAGGGAAAGAACTCCTTTGGGGAAAGCCTGAGGAGTCT CGTGTCTCTAGCGTCTTGGAGGAAAGCAAGCGCCTGGTGGACACCGCCATGTACGCCACGATGCAGAGAAACCTCAAGAAAAGAGGAATCCTTTCTCCAG CTCAGCTTCTGTCTTTTTCCAAACTTCCTGAGCCAACAAGCGGAGTGATTGCCCGAGCAGCAGAGATAATGGAAACATCAATACAAGCGATGAAAAGAAA AGTCAACCTGAAAACTCAACAATCACAGCATCCAACGGATGCTTTATCAGAAGATCTGCTGAGCATCATTGCAAACATGTCTGGATGTCTCCCTTACATG CTGCCCCCAAAATGCCCAAACACTTGCCTGGCGAACAAATACAGGCCCATCACAGGAGCTTGCAACAACAGAGACCACCCCAGATGGGGCGCCTCCAACA CGGCCCTGGCACGATGGCTCCCTCCAGTCTATGAGGACGGCTTCAGTCAGCCCCGAGGCTGGAACCCCGGCTTCTTGTACAACGGGTTCCCACTGCCCCC GGTCCGGGAGGTGACAAGACATGTCATTCAAGTTTCAAATGAGGTTGTCACAGATGATGACCGCTATTCTGACCTCCTGATGGCATGGGGACAATACATC GACCACGACATCGCGTTCACACCACAGAGCACCAGCAAAGCTGCCTTCGGGGGAGGGGCTGACTGCCAGATGACTTGTGAGAACCAAAACCCATGTTTTC CCATACAACTCCCGGAGGAGGCCCGGCCGGCCGCGGGCACCGCCTGTCTGCCCTTCTACCGCTCTTCGGCCGCCTGCGGCACCGGGGACCAAGGCGCGCT CTTTGGGAACCTGTCCACGGCCAACCCGCGGCAGCAGATGAACGGGTTGACCTCGTTCCTGGACGCGTCCACCGTGTATGGCAGCTCCCCGGCCCTAGAG AGGCAGCTGCGGAACTGGACCAGTGCCGAAGGGCTGCTCCGCGTCCACGCGCGCCTCCGGGACTCCGGCCGCGCCTACCTGCCCTTCGTGCCGCCACGCG CGCCTGCGGCCTGTGCGCCCGAGCCCGGCATCCCCGGAGAGACCCGCGGGCCCTGCTTCCTGGCCGGAGACGGCCGCGCCAGCGAGGTCCCCTCCCTGAC GGCACTGCACACGCTGTGGCTGCGCGAGCACAACCGCCTGGCCGCGGCGCTCAAGGCCCTCAATGCGCACTGGAGCGCGGACGCCGTGTACCAGGAGGCG CGCAAGGTCGTGGGCGCTCTGCACCAGATCATCACCCTGAGGGATTACATCCCCAGGATCCTGGGACCCGAGGCCTTCCAGCAGTACGTGGGTCCCTATG AAGGCTATGACTCCACCGCCAACCCCACTGTGTCCAACGTGTTCTCCACAGCCGCCTTCCGCTTCGGCCATGCCACGATCCACCCGCTGGTGAGGAGGCT GGACGCCAGCTTCCAGGAGCACCCCGACCTGCCCGGGCTGTGGCTGCACCAGGCTTTCTTCAGCCCATGGACATTACTCCGTGGAGGTTACAATGAGTGG AGGGAGTTCTGCGGCCTGCCTCGCCTGGAGACCCCCGCTGACCTGAGCACAGCCATCGCCAGCAGGAGCGTGGCCGACAAGATCCTGGACTTGTACAAGC ATCCTGACAACATCGATGTCTGGCTGGGAGGCTTAGCTGAAAACTTCCTCCCCAGGGCTCGGACAGGGCCCCTGTTTGCCTGTCTCATTGGGAAGCAGAT GAAGGCTCTGCGGGACGGTGACTGGTTTTGGTGGGAGAACAGCCACGTCTTCACGGATGCACAGAGGCGTGAGCTGGAGAAGCACTCCCTGTCTCGGGTC ATCTGTGACAACACTGGCCTCACCAGGGTGCCCATGGATGCCTTCCAAGTCGGCAAATTCCCCGAAGACTTTGAGTCTTGTGACAGCATCACTGGCATGA ACCTGGAGGCCTGGAGGGAAACCTTTCCTCAAGACGACAAGTGTGGCTTCCCAGAGAGCGTGGAGAATGGGGACTTTGTGCACTGTGAGGAGTCTGGGAG GCGCGTGCTGGTGTATTCCTGCCGGCACGGGTATGAGCTCCAAGGCCGGGAGCAGCTCACTTGCACCCAGGAAGGATGGGATTTCCAGCCTCCCCTCTGC AAAGATGTGAACGAGTGTGCAGACGGTGCCCACCCCCCCTGCCACGCCTCTGCGAGGTGCAGAAACACCAAAGGCGGCTTCCAGTGTCTCTGCGCGGACC CCTACGAGTTAGGAGACGATGGGAGAACCTGCGTAGACTCCGGGAGGCTCCCTCGGGTGACTTGGATCTCCATGTCGCTGGCTGCTCTGCTGATCGGAGG CTTCGCAGGTCTCACCTCGACGGTGATTTGCAGGTGGACACGCACTGGCACTAAATCCACACTGCCCATCTCGGAGACAGGCGGAGGAACTCCCGAGCTG AGATGCGGAAAGCACCAGGCCGTAGGGACCTCACCGCAGCGGGCCGCAGCTCAGGACTCGGAGCAGGAGAGTGCTGGGATGGAAGGCCGGGATACTCACA GGCTGCCGAGAGCCCTCTGAGGGCAAAGTGGCAGGACACTGCAGAACAGCTTCATGTTCCCAAAATCACCGTACGACTCTTTTCCAAACACAGGCAAATC CGAAATCAGCAGGACGACTGTTTTCCCAACACGGGTAAATCTAGTACCATGTCGTAGTTACTCTCAGGCATGGATGAATAAATGTTATAGCTGCATTTGT CTGGCCTTTTCTTGTAAACATTGCCTGATTTGTTCCTTCTGGGGCTTTGCCATTAAAATGTATTTACAG

refseq_TPO.F4 (forward primer)
refseq_TPO.R4 (reverse primer)
Single exon skipping

Frame 0

KAIKAPISEELQP*KLLSSAVG*EEEKRSE*ER SLCCLSRWLWPAQKPSSPSSREGKNSFGESLRSL VSLASWRKASAWWTPPCTPRCRETSRKEESFLQ LSFCLFPNFLSQQAE*LPEQQR*WKHQYKR*KE KST*KLNNHSIQRMLYQKIC*ASLQTCLDVSLTC CPQNAQTLAWRTNTGPSQELATTETTPDGAPPT RPWHDGSLQSMRTASVSPEAGTPASCTTGSHCP RSGR*QDMSFKFQMRLSQMMTAILTS*WHGDNTS TTTSRSHHRAPAKLPSGEGLTAR*LVRTKTHVF PYNSRRRPGRPRAPPVCPSTALRPPAAPGTKAR SLGTCPRPTRGSR*TG*PRSWTRPPCMAAPRP*R GSCGTGPVPKGCSASTRASGTPAAPTCPSCRHA RLRPVRPSPASPERPAGPASWPETAAPARSPP* RHCTRCGCASTTAWPRRSRPSMRTGARTPCTRRR ARSWALCTRSSP*GITSPGSWDPRPSSSTWVPM KAMTPPPTPLCPTCSPQPPSASAMPRSTRW*GG WTPASRSTPTCPGCGCTRLSSAHGHYSVEVTMSG GSSAACLAWRPPLT*AQPSPAGAWPTRSWTCTS ILTTSMSGWEA*LKTSSPGLGQGPCLPVSLGSR *RLCGTVTGFGGRTATSSRMHRGVSWRSTPCLGS SVTTLASPGCPWMPSKSANSPKTLSLVTASLA* TWRPGGKPFLKTTSVASQRAWRMGTLCTVRSLG GACWCIPAGTGMSSKAGSSSLAPRKDGISSLPSA KM*TSVQTVPTPPATPLRGAETPKAASSVSART PTS*ETMGEPA*TPGGSLG*LGSPCRWLLC*SE ASQVSPRR*FAGGHALALNPHCPSRRQAEELPS* DAESTRP*GPHRSGPQLRTRSRRVLGWKAGILT GCREPSEGKVAGHCRTASCSQNHRTTLFQTQAN PKSAGRLFSQHG*I*YHVVVTLRHG*INVIAAFV WPFLVNIA*FVPSGALPLKCIY

Frame 1

RQLRRPFQKSYSRENYSAVQLAEKRKKGQNESA RCAVCHAGYGLHRSLLPLHLERERTPLGKA*GV SCL*RLGGKQAPGGHRHVRHDAEKPQEKRNPFSS SASVFFQTS*ANKRSDCPSSRDNGNINTSDEKK SQPENSTITASNGCFIRRSAEHHCKHVWMSPLH AAPKMPKHLPGEQIQAHHRSLQQQRPPQMGRLQH GPGTMAPSSL*GRLQSAPRLEPRLLVQRVPTAP GPGGDKTCHSSFK*GCHR**PLF*PPDGMGTIH RPRHRVHTTEHQQSCLRGRG*LPDDL*EPKPMFS HTTPGGGPAGRGHRLSALLPLFGRLRHRGPRRA LWEPVHGQPAAADERVDLVPGRVHRVWQLPGPR EAAAELDQCRRAAPRPRAPPGLRPRLPALRAATR ACGLCARARHPRRDPRALLPGRRRPRQRGPLPD GTAHAVAARAQPPGRGAQGPQCALERGRRVPGG AQGRGRSAPDHHPEGLHPQDPGTRGLPAVRGSL* RL*LHRQPHCVQRVLHSRLPLRPCHDPPAGEEA GRQLPGAPRPARAVAAPGFLQPMDITPWRLQ*V EGVLRPASPGDPR*PEHSHRQQERGRQDPGLVQA S*QHRCLAGRLS*KLPPQGSDRAPVCLSHWEAD EGSAGR*LVLVGEQPRLHGCTEA*AGEALPVSG HL*QHWPHQGAHGCLPSRQIPRRL*VL*QHHWHE PGGLEGNLSSRRQVWLPRERGEWGLCAL*GVWE ARAGVFLPARV*APRPGAAHLHPGRMGFPASPL QRCERVCRRCPPPLPRLCEVQKHQRRLPVSLRGP LRVRRRWENLRRLREAPSGDLDLHVAGCSADRR LRRSHLDGDLQVDTHWH*IHTAHLGDRRRNSRA EMRKAPGRRDLTAAGRSSGLGAGECWDGRPGYSQ AAESPLRAKWQDTAEQLHVPKITVRLFSKHRQI RNQQDDCFPNTGKSSTMS*LLSGMDE*ML*LHL SGLFL*TLPDLFLLGLCH*NVFT

Frame 2

GN*GAHFRRVTAVKITQQCSWLRRGKKVRMRAL AVLSVTLVMACTEAFFPFISRGKELLWGKPEES RVSSVLEESKRLVDTAMYATMQRNLKKRGILSP AQLLSFSKLPEPTSGVIARAAEIMETSIQAMKRK VNLKTQQSQHPTDALSEDLLSIIANMSGCLPYM LPPKCPNTCLANKYRPITGACNNRDHPRWGASN TALARWLPPVYEDGFSQPRGWNPGFLYNGFPLPP VREVTRHVIQVSNEVVTDDDRYSDLLMAWGQYI DHDIAFTPQSTSKAAFGGGADCQMTCENQNPCF PIQLPEEARPAAGTACLPFYRSSAACGTGDQGAL FGNLSTANPRQQMNGLTSFLDASTVYGSSPALE RQLRNWTSAEGLLRVHARLRDSGRAYLPFVPPR APAACAPEPGIPGETRGPCFLAGDGRASEVPSLT ALHTLWLREHNRLAAALKALNAHWSADAVYQEA RKVVGALHQIITLRDYIPRILGPEAFQQYVGPY EGYDSTANPTVSNVFSTAAFRFGHATIHPLVRRL DASFQEHPDLPGLWLHQAFFSPWTLLRGGYNEW REFCGLPRLETPADLSTAIASRSVADKILDLYK HPDNIDVWLGGLAENFLPRARTGPLFACLIGKQM KALRDGDWFWWENSHVFTDAQRRELEKHSLSRV ICDNTGLTRVPMDAFQVGKFPEDFESCDSITGM NLEAWRETFPQDDKCGFPESVENGDFVHCEESGR RVLVYSCRHGYELQGREQLTCTQEGWDFQPPLC KDVNECADGAHPPCHASARCRNTKGGFQCLCAD PYELGDDGRTCVDSGRLPRVTWISMSLAALLIGG FAGLTSTVICRWTRTGTKSTLPISETGGGTPEL RCGKHQAVGTSPQRAAAQDSEQESAGMEGRDTH RLPRAL*GQSGRTLQNSFMFPKSPYDSFPNTGKS EISRTTVFPTRVNLVPCRSYSQAWMNKCYSCIC LAFSCKHCLICSFWGFAIKMYLQ

Sequence NM_175720

AAGGCAATTAAGGCGCCCATTTCAGAAGAGTTACAGCCGTGAAAATTACTCAGCAGTGCAGTTGGCTGAGAAGAGGAAAAAAGAATGAGAGCGCTCGCTG TGCTGTCTGTCACGCTGGTTATGGCCTGCACAGAAGCCTTCTTCCCCTTCATCTCGAGAGGGAAAGAACTCCTTTGGGGAAAGCCTGAGGAGTCTCGTGT CTCTAGCGTCTTGGAGGAAAGCAAGCGCCTGGTGGACACCGCCATGTACGCCACGATGCAGAGAAACCTCAAGAAAAGAGGAATCCTTTCTCCAGCTCAG CTTCTGTCTTTTTCCAAACTTCCTGAGCCAACAAGCGGAGTGATTGCCCGAGCAGCAGAGATAATGGAAACATCAATACAAGCGATGAAAAGAAAAGTCA ACCTGAAAACTCAACAATCACAGCATCCAACGGATGCTTTATCAGAAGATCTGCTGAGCATCATTGCAAACATGTCTGGATGTCTCCCTTACATGCTGCC CCCAAAATGCCCAAACACTTGCCTGGCGAACAAATACAGGCCCATCACAGGAGCTTGCAACAACAGAGACCACCCCAGATGGGGCGCCTCCAACACGGCC CTGGCACGATGGCTCCCTCCAGTCTATGAGGACGGCTTCAGTCAGCCCCGAGGCTGGAACCCCGGCTTCTTGTACAACGGGTTCCCACTGCCCCCGGTCC GGGAGGTGACAAGACATGTCATTCAAGTTTCAAATGAGGTTGTCACAGATGATGACCGCTATTCTGACCTCCTGATGGCATGGGGACAATACATCGACCA CGACATCGCGTTCACACCACAGAGCACCAGCAAAGCTGCCTTCGGGGGAGGGGCTGACTGCCAGATGACTTGTGAGAACCAAAACCCATGTTTTCCCATA CAACTCCCGGAGGAGGCCCGGCCGGCCGCGGGCACCGCCTGTCTGCCCTTCTACCGCTCTTCGGCCGCCTGCGGCACCGGGGACCAAGGCGCGCTCTTTG GGAACCTGTCCACGGCCAACCCGCGGCAGCAGATGAACGGGTTGACCTCGTTCCTGGACGCGTCCACCGTGTATGGCAGCTCCCCGGCCCTAGAGAGGCA GCTGCGGAACTGGACCAGTGCCGAAGGGCTGCTCCGCGTCCACGCGCGCCTCCGGGACTCCGGCCGCGCCTACCTGCCCTTCGTGCCGCCACGCGCGCCT GCGGCCTGTGCGCCCGAGCCCGGCATCCCCGGAGAGACCCGCGGGCCCTGCTTCCTGGCCGGAGACGGCCGCGCCAGCGAGGTCCCCTCCCTGACGGCAC TGCACACGCTGTGGCTGCGCGAGCACAACCGCCTGGCCGCGGCGCTCAAGGCCCTCAATGCGCACTGGAGCGCGGACGCCGTGTACCAGGAGGCGCGCAA GGTCGTGGGCGCTCTGCACCAGATCATCACCCTGAGGGATTACATCCCCAGGATCCTGGGACCCGAGGCCTTCCAGCAGTACGTGGGTCCCTATGAAGGC TATGACTCCACCGCCAACCCCACTGTGTCCAACGTGTTCTCCACAGCCGCCTTCCGCTTCGGCCATGCCACGATCCACCCGCTGGTGAGGAGGCTGGACG CCAGCTTCCAGGAGCACCCCGACCTGCCCGGGCTGTGGCTGCACCAGGCTTTCTTCAGCCCATGGACATTACTCCGTGGAGGTGGTTTGGACCCACTAAT ACGAGGCCTTCTTGCAAGACCAGCCAAACTGCAGGTGCAGGATCAGCTGATGAACGAGGAGCTGACGGAAAGGCTCTTTGTGCTGTCCAATTCCAGCACC TTGGATCTGGCGTCCATCAACCTGCAGAGGGGCCGGGACCACGGGCTGCCAGGTTACAATGAGTGGAGGGAGTTCTGCGGCCTGCCTCGCCTGGAGACCC CCGCTGACCTGAGCACAGCCATCGCCAGCAGGAGCGTGGCCGACAAGATCCTGGACTTGTACAAGCATCCTGACAACATCGATGTCTGGCTGGGAGGCTT AGCTGAAAACTTCCTCCCCAGGGCTCGGACAGGGCCCCTGTTTGCCTGTCTCATTGGGAAGCAGATGAAGGCTCTGCGGGACGGTGACTGGTTTTGGTGG GAGAACAGCCACGTCTTCACGGATGCACAGAGGCGTGAGCTGGAGAAGCACTCCCTGTCTCGGGTCATCTGTGACAACACTGGCCTCACCAGGGTGCCCA TGGATGCCTTCCAAGTCGGCAAATTCCCCGAAGACTTTGAGTCTTGTGACAGCATCACTGGCATGAACCTGGAGGCCTGGAGGGAAACCTTTCCTCAAGA CGACAAGTGTGGCTTCCCAGAGAGCGTGGAGAATGGGGACTTTGTGCACTGTGAGGAGTCTGGGAGGCGCGTGCTGGTGTATTCCTGCCGGCACGGGTAT GAGCTCCAAGGCCGGGAGCAGCTCACTTGCACCCAGGAAGGATGGGATTTCCAGCCTCCCCTCTGCAAAGATGTGAACGAGTGTGCAGACGGTGCCCACC CCCCCTGCCACGCCTCTGCGAGGTGCAGAAACACCAAAGGCGGCTTCCAGTGTCTCTGCGCGGACCCCTACGAGTTAGGAGACGATGGGAGAACCTGCGT AGACTCCGGGAGGCTCCCTCGGGTGACTTGGATCTCCATGTCGCTGGCTGCTCTGCTGATCGGAGGCTTCGCAGGTCTCACCTCGACGGTGATTTGCAGG TGGAGAGTGCTGGGATGGAAGGCCGGGATACTCACAGGCTGCCGAGAGCCCTCTGAGGGCAAAGTGGCAGGACACTGCAGAACAGCTTCATGTTCCCAAA ATCACCGTACGACTCTTTTCCAAACACAGGCAAATCCGAAATCAGCAGGACGACTGTTTTCCCAACACGGGTAAATCTAGTACCATGTCGTAGTTACTCT CAGGCATGGATGAATAAATGTTATAGCTGCATTTGTCTGGCCTTTTCTTGTAAACATTGCCTGATTTGTTCCTTCTGGGGCTTTGCCATTAAAATGTATT TACAG

refseq_TPO.F4 (forward primer)
refseq_TPO.R4 (reverse primer)
Single exon skipping

Frame 0

KAIKAPISEELQP*KLLSSAVG*EEEKRMRALA VLSVTLVMACTEAFFPFISRGKELLWGKPEESRV SSVLEESKRLVDTAMYATMQRNLKKRGILSPAQ LLSFSKLPEPTSGVIARAAEIMETSIQAMKRKV NLKTQQSQHPTDALSEDLLSIIANMSGCLPYMLP PKCPNTCLANKYRPITGACNNRDHPRWGASNTA LARWLPPVYEDGFSQPRGWNPGFLYNGFPLPPV REVTRHVIQVSNEVVTDDDRYSDLLMAWGQYIDH DIAFTPQSTSKAAFGGGADCQMTCENQNPCFPI QLPEEARPAAGTACLPFYRSSAACGTGDQGALF GNLSTANPRQQMNGLTSFLDASTVYGSSPALERQ LRNWTSAEGLLRVHARLRDSGRAYLPFVPPRAP AACAPEPGIPGETRGPCFLAGDGRASEVPSLTA LHTLWLREHNRLAAALKALNAHWSADAVYQEARK VVGALHQIITLRDYIPRILGPEAFQQYVGPYEG YDSTANPTVSNVFSTAAFRFGHATIHPLVRRLD ASFQEHPDLPGLWLHQAFFSPWTLLRGGGLDPLI RGLLARPAKLQVQDQLMNEELTERLFVLSNSST LDLASINLQRGRDHGLPGYNEWREFCGLPRLET PADLSTAIASRSVADKILDLYKHPDNIDVWLGGL AENFLPRARTGPLFACLIGKQMKALRDGDWFWW ENSHVFTDAQRRELEKHSLSRVICDNTGLTRVP MDAFQVGKFPEDFESCDSITGMNLEAWRETFPQD DKCGFPESVENGDFVHCEESGRRVLVYSCRHGY ELQGREQLTCTQEGWDFQPPLCKDVNECADGAH PPCHASARCRNTKGGFQCLCADPYELGDDGRTCV DSGRLPRVTWISMSLAALLIGGFAGLTSTVICR WRVLGWKAGILTGCREPSEGKVAGHCRTASCSQ NHRTTLFQTQANPKSAGRLFSQHG*I*YHVVVTL RHG*INVIAAFVWPFLVNIA*FVPSGALPLKCI Y

Frame 1

RQLRRPFQKSYSRENYSAVQLAEKRKKE*ERSL CCLSRWLWPAQKPSSPSSREGKNSFGESLRSLV SLASWRKASAWWTPPCTPRCRETSRKEESFLQLS FCLFPNFLSQQAE*LPEQQR*WKHQYKR*KEKS T*KLNNHSIQRMLYQKIC*ASLQTCLDVSLTCC PQNAQTLAWRTNTGPSQELATTETTPDGAPPTRP WHDGSLQSMRTASVSPEAGTPASCTTGSHCPRS GR*QDMSFKFQMRLSQMMTAILTS*WHGDNTST TTSRSHHRAPAKLPSGEGLTAR*LVRTKTHVFPY NSRRRPGRPRAPPVCPSTALRPPAAPGTKARSL GTCPRPTRGSR*TG*PRSWTRPPCMAAPRP*RG SCGTGPVPKGCSASTRASGTPAAPTCPSCRHARL RPVRPSPASPERPAGPASWPETAAPARSPP*RH CTRCGCASTTAWPRRSRPSMRTGARTPCTRRRA RSWALCTRSSP*GITSPGSWDPRPSSSTWVPMKA MTPPPTPLCPTCSPQPPSASAMPRSTRW*GGWT PASRSTPTCPGCGCTRLSSAHGHYSVEVVWTH* YEAFLQDQPNCRCRIS**TRS*RKGSLCCPIPAP WIWRPSTCRGAGTTGCQVTMSGGSSAACLAWRP PLT*AQPSPAGAWPTRSWTCTSILTTSMSGWEA *LKTSSPGLGQGPCLPVSLGSR*RLCGTVTGFGG RTATSSRMHRGVSWRSTPCLGSSVTTLASPGCP WMPSKSANSPKTLSLVTASLA*TWRPGGKPFLK TTSVASQRAWRMGTLCTVRSLGGACWCIPAGTGM SSKAGSSSLAPRKDGISSLPSAKM*TSVQTVPT PPATPLRGAETPKAASSVSARTPTS*ETMGEPA *TPGGSLG*LGSPCRWLLC*SEASQVSPRR*FAG GECWDGRPGYSQAAESPLRAKWQDTAEQLHVPK ITVRLFSKHRQIRNQQDDCFPNTGKSSTMS*LL SGMDE*ML*LHLSGLFL*TLPDLFLLGLCH*NVF T

Frame 2

GN*GAHFRRVTAVKITQQCSWLRRGKKNESARC AVCHAGYGLHRSLLPLHLERERTPLGKA*GVSC L*RLGGKQAPGGHRHVRHDAEKPQEKRNPFSSS ASVFFQTS*ANKRSDCPSSRDNGNINTSDEKKSQ PENSTITASNGCFIRRSAEHHCKHVWMSPLHAA PKMPKHLPGEQIQAHHRSLQQQRPPQMGRLQHG PGTMAPSSL*GRLQSAPRLEPRLLVQRVPTAPGP GGDKTCHSSFK*GCHR**PLF*PPDGMGTIHRP RHRVHTTEHQQSCLRGRG*LPDDL*EPKPMFSH TTPGGGPAGRGHRLSALLPLFGRLRHRGPRRALW EPVHGQPAAADERVDLVPGRVHRVWQLPGPREA AAELDQCRRAAPRPRAPPGLRPRLPALRAATRA CGLCARARHPRRDPRALLPGRRRPRQRGPLPDGT AHAVAARAQPPGRGAQGPQCALERGRRVPGGAQ GRGRSAPDHHPEGLHPQDPGTRGLPAVRGSL*R L*LHRQPHCVQRVLHSRLPLRPCHDPPAGEEAGR QLPGAPRPARAVAAPGFLQPMDITPWRWFGPTN TRPSCKTSQTAGAGSADERGADGKALCAVQFQH LGSGVHQPAEGPGPRAARLQ*VEGVLRPASPGDP R*PEHSHRQQERGRQDPGLVQAS*QHRCLAGRL S*KLPPQGSDRAPVCLSHWEADEGSAGR*LVLV GEQPRLHGCTEA*AGEALPVSGHL*QHWPHQGAH GCLPSRQIPRRL*VL*QHHWHEPGGLEGNLSSR RQVWLPRERGEWGLCAL*GVWEARAGVFLPARV *APRPGAAHLHPGRMGFPASPLQRCERVCRRCPP PLPRLCEVQKHQRRLPVSLRGPLRVRRRWENLR RLREAPSGDLDLHVAGCSADRRLRRSHLDGDLQ VESAGMEGRDTHRLPRAL*GQSGRTLQNSFMFPK SPYDSFPNTGKSEISRTTVFPTRVNLVPCRSYS QAWMNKCYSCICLAFSCKHCLICSFWGFAIKMY LQ

Sequence NM_000547

AAGGCAATTAAGGCGCCCATTTCAGAAGAGTTACAGCCGTGAAAATTACTCAGCAGTGCAGTTGGCTGAGAAGAGGAAAAAAGAATGAGAGCGCTCGCTG TGCTGTCTGTCACGCTGGTTATGGCCTGCACAGAAGCCTTCTTCCCCTTCATCTCGAGAGGGAAAGAACTCCTTTGGGGAAAGCCTGAGGAGTCTCGTGT CTCTAGCGTCTTGGAGGAAAGCAAGCGCCTGGTGGACACCGCCATGTACGCCACGATGCAGAGAAACCTCAAGAAAAGAGGAATCCTTTCTCCAGCTCAG CTTCTGTCTTTTTCCAAACTTCCTGAGCCAACAAGCGGAGTGATTGCCCGAGCAGCAGAGATAATGGAAACATCAATACAAGCGATGAAAAGAAAAGTCA ACCTGAAAACTCAACAATCACAGCATCCAACGGATGCTTTATCAGAAGATCTGCTGAGCATCATTGCAAACATGTCTGGATGTCTCCCTTACATGCTGCC CCCAAAATGCCCAAACACTTGCCTGGCGAACAAATACAGGCCCATCACAGGAGCTTGCAACAACAGAGACCACCCCAGATGGGGCGCCTCCAACACGGCC CTGGCACGATGGCTCCCTCCAGTCTATGAGGACGGCTTCAGTCAGCCCCGAGGCTGGAACCCCGGCTTCTTGTACAACGGGTTCCCACTGCCCCCGGTCC GGGAGGTGACAAGACATGTCATTCAAGTTTCAAATGAGGTTGTCACAGATGATGACCGCTATTCTGACCTCCTGATGGCATGGGGACAATACATCGACCA CGACATCGCGTTCACACCACAGAGCACCAGCAAAGCTGCCTTCGGGGGAGGGGCTGACTGCCAGATGACTTGTGAGAACCAAAACCCATGTTTTCCCATA CAACTCCCGGAGGAGGCCCGGCCGGCCGCGGGCACCGCCTGTCTGCCCTTCTACCGCTCTTCGGCCGCCTGCGGCACCGGGGACCAAGGCGCGCTCTTTG GGAACCTGTCCACGGCCAACCCGCGGCAGCAGATGAACGGGTTGACCTCGTTCCTGGACGCGTCCACCGTGTATGGCAGCTCCCCGGCCCTAGAGAGGCA GCTGCGGAACTGGACCAGTGCCGAAGGGCTGCTCCGCGTCCACGCGCGCCTCCGGGACTCCGGCCGCGCCTACCTGCCCTTCGTGCCGCCACGCGCGCCT GCGGCCTGTGCGCCCGAGCCCGGCATCCCCGGAGAGACCCGCGGGCCCTGCTTCCTGGCCGGAGACGGCCGCGCCAGCGAGGTCCCCTCCCTGACGGCAC TGCACACGCTGTGGCTGCGCGAGCACAACCGCCTGGCCGCGGCGCTCAAGGCCCTCAATGCGCACTGGAGCGCGGACGCCGTGTACCAGGAGGCGCGCAA GGTCGTGGGCGCTCTGCACCAGATCATCACCCTGAGGGATTACATCCCCAGGATCCTGGGACCCGAGGCCTTCCAGCAGTACGTGGGTCCCTATGAAGGC TATGACTCCACCGCCAACCCCACTGTGTCCAACGTGTTCTCCACAGCCGCCTTCCGCTTCGGCCATGCCACGATCCACCCGCTGGTGAGGAGGCTGGACG CCAGCTTCCAGGAGCACCCCGACCTGCCCGGGCTGTGGCTGCACCAGGCTTTCTTCAGCCCATGGACATTACTCCGTGGAGGTGGTTTGGACCCACTAAT ACGAGGCCTTCTTGCAAGACCAGCCAAACTGCAGGTGCAGGATCAGCTGATGAACGAGGAGCTGACGGAAAGGCTCTTTGTGCTGTCCAATTCCAGCACC TTGGATCTGGCGTCCATCAACCTGCAGAGGGGCCGGGACCACGGGCTGCCAGGTTACAATGAGTGGAGGGAGTTCTGCGGCCTGCCTCGCCTGGAGACCC CCGCTGACCTGAGCACAGCCATCGCCAGCAGGAGCGTGGCCGACAAGATCCTGGACTTGTACAAGCATCCTGACAACATCGATGTCTGGCTGGGAGGCTT AGCTGAAAACTTCCTCCCCAGGGCTCGGACAGGGCCCCTGTTTGCCTGTCTCATTGGGAAGCAGATGAAGGCTCTGCGGGACGGTGACTGGTTTTGGTGG GAGAACAGCCACGTCTTCACGGATGCACAGAGGCGTGAGCTGGAGAAGCACTCCCTGTCTCGGGTCATCTGTGACAACACTGGCCTCACCAGGGTGCCCA TGGATGCCTTCCAAGTCGGCAAATTCCCCGAAGACTTTGAGTCTTGTGACAGCATCACTGGCATGAACCTGGAGGCCTGGAGGGAAACCTTTCCTCAAGA CGACAAGTGTGGCTTCCCAGAGAGCGTGGAGAATGGGGACTTTGTGCACTGTGAGGAGTCTGGGAGGCGCGTGCTGGTGTATTCCTGCCGGCACGGGTAT GAGCTCCAAGGCCGGGAGCAGCTCACTTGCACCCAGGAAGGATGGGATTTCCAGCCTCCCCTCTGCAAAGATGTGAACGAGTGTGCAGACGGTGCCCACC CCCCCTGCCACGCCTCTGCGAGGTGCAGAAACACCAAAGGCGGCTTCCAGTGTCTCTGCGCGGACCCCTACGAGTTAGGAGACGATGGGAGAACCTGCGT AGACTCCGGGAGGCTCCCTCGGGTGACTTGGATCTCCATGTCGCTGGCTGCTCTGCTGATCGGAGGCTTCGCAGGTCTCACCTCGACGGTGATTTGCAGG TGGACACGCACTGGCACTAAATCCACACTGCCCATCTCGGAGACAGGCGGAGGAACTCCCGAGCTGAGATGCGGAAAGCACCAGGCCGTAGGGACCTCAC CGCAGCGGGCCGCAGCTCAGGACTCGGAGCAGGAGAGTGCTGGGATGGAAGGCCGGGATACTCACAGGCTGCCGAGAGCCCTCTGAGGGCAAAGTGGCAG GACACTGCAGAACAGCTTCATGTTCCCAAAATCACCGTACGACTCTTTTCCAAACACAGGCAAATCCGAAATCAGCAGGACGACTGTTTTCCCAACACGG GTAAATCTAGTACCATGTCGTAGTTACTCTCAGGCATGGATGAATAAATGTTATAGCTGCATTTGTCTGGCCTTTTCTTGTAAACATTGCCTGATTTGTT CCTTCTGGGGCTTTGCCATTAAAATGTATTTACAG

refseq_TPO.F4 (forward primer)
refseq_TPO.R4 (reverse primer)
Single exon skipping

Frame 0

KAIKAPISEELQP*KLLSSAVG*EEEKRMRALA VLSVTLVMACTEAFFPFISRGKELLWGKPEESRV SSVLEESKRLVDTAMYATMQRNLKKRGILSPAQ LLSFSKLPEPTSGVIARAAEIMETSIQAMKRKV NLKTQQSQHPTDALSEDLLSIIANMSGCLPYMLP PKCPNTCLANKYRPITGACNNRDHPRWGASNTA LARWLPPVYEDGFSQPRGWNPGFLYNGFPLPPV REVTRHVIQVSNEVVTDDDRYSDLLMAWGQYIDH DIAFTPQSTSKAAFGGGADCQMTCENQNPCFPI QLPEEARPAAGTACLPFYRSSAACGTGDQGALF GNLSTANPRQQMNGLTSFLDASTVYGSSPALERQ LRNWTSAEGLLRVHARLRDSGRAYLPFVPPRAP AACAPEPGIPGETRGPCFLAGDGRASEVPSLTA LHTLWLREHNRLAAALKALNAHWSADAVYQEARK VVGALHQIITLRDYIPRILGPEAFQQYVGPYEG YDSTANPTVSNVFSTAAFRFGHATIHPLVRRLD ASFQEHPDLPGLWLHQAFFSPWTLLRGGGLDPLI RGLLARPAKLQVQDQLMNEELTERLFVLSNSST LDLASINLQRGRDHGLPGYNEWREFCGLPRLET PADLSTAIASRSVADKILDLYKHPDNIDVWLGGL AENFLPRARTGPLFACLIGKQMKALRDGDWFWW ENSHVFTDAQRRELEKHSLSRVICDNTGLTRVP MDAFQVGKFPEDFESCDSITGMNLEAWRETFPQD DKCGFPESVENGDFVHCEESGRRVLVYSCRHGY ELQGREQLTCTQEGWDFQPPLCKDVNECADGAH PPCHASARCRNTKGGFQCLCADPYELGDDGRTCV DSGRLPRVTWISMSLAALLIGGFAGLTSTVICR WTRTGTKSTLPISETGGGTPELRCGKHQAVGTS PQRAAAQDSEQESAGMEGRDTHRLPRAL*GQSGR TLQNSFMFPKSPYDSFPNTGKSEISRTTVFPTR VNLVPCRSYSQAWMNKCYSCICLAFSCKHCLIC SFWGFAIKMYLQ

Frame 1

RQLRRPFQKSYSRENYSAVQLAEKRKKE*ERSL CCLSRWLWPAQKPSSPSSREGKNSFGESLRSLV SLASWRKASAWWTPPCTPRCRETSRKEESFLQLS FCLFPNFLSQQAE*LPEQQR*WKHQYKR*KEKS T*KLNNHSIQRMLYQKIC*ASLQTCLDVSLTCC PQNAQTLAWRTNTGPSQELATTETTPDGAPPTRP WHDGSLQSMRTASVSPEAGTPASCTTGSHCPRS GR*QDMSFKFQMRLSQMMTAILTS*WHGDNTST TTSRSHHRAPAKLPSGEGLTAR*LVRTKTHVFPY NSRRRPGRPRAPPVCPSTALRPPAAPGTKARSL GTCPRPTRGSR*TG*PRSWTRPPCMAAPRP*RG SCGTGPVPKGCSASTRASGTPAAPTCPSCRHARL RPVRPSPASPERPAGPASWPETAAPARSPP*RH CTRCGCASTTAWPRRSRPSMRTGARTPCTRRRA RSWALCTRSSP*GITSPGSWDPRPSSSTWVPMKA MTPPPTPLCPTCSPQPPSASAMPRSTRW*GGWT PASRSTPTCPGCGCTRLSSAHGHYSVEVVWTH* YEAFLQDQPNCRCRIS**TRS*RKGSLCCPIPAP WIWRPSTCRGAGTTGCQVTMSGGSSAACLAWRP PLT*AQPSPAGAWPTRSWTCTSILTTSMSGWEA *LKTSSPGLGQGPCLPVSLGSR*RLCGTVTGFGG RTATSSRMHRGVSWRSTPCLGSSVTTLASPGCP WMPSKSANSPKTLSLVTASLA*TWRPGGKPFLK TTSVASQRAWRMGTLCTVRSLGGACWCIPAGTGM SSKAGSSSLAPRKDGISSLPSAKM*TSVQTVPT PPATPLRGAETPKAASSVSARTPTS*ETMGEPA *TPGGSLG*LGSPCRWLLC*SEASQVSPRR*FAG GHALALNPHCPSRRQAEELPS*DAESTRP*GPH RSGPQLRTRSRRVLGWKAGILTGCREPSEGKVA GHCRTASCSQNHRTTLFQTQANPKSAGRLFSQHG *I*YHVVVTLRHG*INVIAAFVWPFLVNIA*FV PSGALPLKCIY

Frame 2

GN*GAHFRRVTAVKITQQCSWLRRGKKNESARC AVCHAGYGLHRSLLPLHLERERTPLGKA*GVSC L*RLGGKQAPGGHRHVRHDAEKPQEKRNPFSSS ASVFFQTS*ANKRSDCPSSRDNGNINTSDEKKSQ PENSTITASNGCFIRRSAEHHCKHVWMSPLHAA PKMPKHLPGEQIQAHHRSLQQQRPPQMGRLQHG PGTMAPSSL*GRLQSAPRLEPRLLVQRVPTAPGP GGDKTCHSSFK*GCHR**PLF*PPDGMGTIHRP RHRVHTTEHQQSCLRGRG*LPDDL*EPKPMFSH TTPGGGPAGRGHRLSALLPLFGRLRHRGPRRALW EPVHGQPAAADERVDLVPGRVHRVWQLPGPREA AAELDQCRRAAPRPRAPPGLRPRLPALRAATRA CGLCARARHPRRDPRALLPGRRRPRQRGPLPDGT AHAVAARAQPPGRGAQGPQCALERGRRVPGGAQ GRGRSAPDHHPEGLHPQDPGTRGLPAVRGSL*R L*LHRQPHCVQRVLHSRLPLRPCHDPPAGEEAGR QLPGAPRPARAVAAPGFLQPMDITPWRWFGPTN TRPSCKTSQTAGAGSADERGADGKALCAVQFQH LGSGVHQPAEGPGPRAARLQ*VEGVLRPASPGDP R*PEHSHRQQERGRQDPGLVQAS*QHRCLAGRL S*KLPPQGSDRAPVCLSHWEADEGSAGR*LVLV GEQPRLHGCTEA*AGEALPVSGHL*QHWPHQGAH GCLPSRQIPRRL*VL*QHHWHEPGGLEGNLSSR RQVWLPRERGEWGLCAL*GVWEARAGVFLPARV *APRPGAAHLHPGRMGFPASPLQRCERVCRRCPP PLPRLCEVQKHQRRLPVSLRGPLRVRRRWENLR RLREAPSGDLDLHVAGCSADRRLRRSHLDGDLQ VDTHWH*IHTAHLGDRRRNSRAEMRKAPGRRDLT AAGRSSGLGAGECWDGRPGYSQAAESPLRAKWQ DTAEQLHVPKITVRLFSKHRQIRNQQDDCFPNT GKSSTMS*LLSGMDE*ML*LHLSGLFL*TLPDLF LLGLCH*NVFT