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Sequences

Sequence NM_201624

GGAGACACCGGAAGGAGCCGCGGCTGCTGCGGGAAGTGGCCAGTTCAGGAGGCGGACCCCCCGAGGGCAGCGCTGCGGGGCCGTTTTCCGGCCCTCCTGA CGCGACACTGCCCCTCTCCGAGAGCTGAGAAGGAAAAGAGGAGCTTGCGGAGGTGCGGCTGCAGGCCGTTGTTGGTCGAGCTGGCGGGTCCCGCGGGCCA GGCCGTGGAGGTGTTACCTCATTTTGAAAGTCTTGGGAAACAGGAAAAAATTCCTAACAAAATGTCAGCTTTTCGAAATCATTGTCCACATTTGGATTCA GTTGGTGAAATAACAAAAGAAGATTTGATACAAAAATCCCTTGGTACTTGTCAGGATTGTAAAGTCCAAGGACCAAATCTTTGGGCATGTCTGGAGAATA GATGTTCATATGTTGGCTGTGGTGAATCACAAGTAGATCACAGCACCATACATTCTCAGGAGACAAAGCATTATCTAACTGTGAACCTTACCACTCTTCG AGTATGGTGTTATGCTTGCAGCAAAGAAGTATTTTTGGATAGGAAATTAGGAACTCAGCCTTCATTGCCTCATGTAAGACAACCTCACCAAATACAAGAA AACAGTGTCCAGGATTTTAAAATACCCAGTAATACAACATTAAAAACTCCTCTGGTTGCCGTATTTGATGATCTGGATATAGAAGCGGATGAAGAAGATG AACTTAGGGCCAGAGGTCTTACAGGTTTGAAAAATATTGGAAATACTTGTTACATGAATGCAGCTTTGCAGGCTCTTTCTAATTGCCCACCTTTGACACA GTTTTTTCTTGATTGTGGAGGACTAGCTCGAACAGATAAGAAACCTGCCATTTGTAAAAGTTATCTCAAACTAATGACAGAGCTGTGGCATAAAAGCAGG CCAGGATCTGTTGTGCCTACTACTCTGTTTCAAGGAATTAAAACTGTAAATCCAACATTTCGGGGGTATTCTCAGCAGGATGCTCAAGAATTCCTTCGAT GTTTAATGGATTTGCTTCATGAAGAATTGAAAGAGCAAGTCATGGAAGTAGAAGAAGATCCGCAAACCATAACCACTGAGGAGACAATGGAAGAAGACAA GAGCCAGTCGGATGTAGATTTTCAGTCTTGTGAATCTTGTAGCAACAGTGATAGAGCAGAAAATGAAAATGGCTCTAGATGCTTTTCTGAAGATAATAAT GAAACAACAATGTTAATTCAGGATGATGAAAACAATTCAGAAATGTCAAAGGATTGGCAAAAAGAGAAGATGTGCAATAAGATTAATAAAGTAAATTCTG AAGGCGAATTTGATAAAGATAGAGACTCTATATCTGAAACAGTCGACTTAAACAACCAGGAAACTGTCAAAGTGCAAATACACAGCAGAGCTTCAGAATA TATCACTGATGTCCATTCGAATGACCTGTCTACACCACAGATCCTTCCATCAAATGAAGGTGTTAATCCACGTTTATCGGCAAGCCCTCCTAAATCAGGC AATTTGTGGCCAGGATTGGCACCACCACACAAAAAAGCTCAGTCTGCATCTCCAAAGAGAAAAAAACAGCACAAGAAATACAGAAGTGTTATTTCAGACA TATTTGATGGAACAATCATTAGTTCAGTGCAGTGTCTGACTTGTGACAGGGTGTCTGTAACCCTCGAGACCTTTCAAGATCTGTCCTTGCCAATTCCTGG CAAGGAAGACCTTGCTAAGCTGCATTCATCAAGTCATCCAACTTCTATAGTCAAAGCAGGATCATGTGGCGAAGCATATGCTCCACAAGGGTGGATAGCT TTTTTCATGGAATATGTGAAGAGGTTTGTTGTCTCATGTGTCCCTAGCTGGTTTTGGGGTCCAGTAGTAACCTTGCAAGATTGTCTTGCTGCCTTCTTTG CCAGAGATGAACTAAAAGGTGACAATATGTACAGTTGTGAAAAATGCAAAAAGTTGAGAAATGGAGTGAAGTTTTGTAAAGTACAAAACTTTCCTGAGAT TTTGTGCATCCACCTTAAAAGATTCAGACATGAACTAATGTTTTCCACCAAAATCAGTACCCATGTTTCATTTCCGCTAGAAGGCTTGGATCTTCAGCCA TTTCTTGCTAAGGATAGTCCAGCTCAAATTGTGACATATGATCTTCTGTCAGTCATTTGCCATCATGGAACTGCAAGTAGTGGACACTATATAGCCTACT GCCGAAACAATCTAAATAATCTCTGGTATGAATTTGATGATCAGAGTGTCACTGAAGTTTCAGAATCTACTGTACAAAATGCAGAAGCTTACGTTCTTTT CTATAGGAAGAGCAGCGAAGAGGCACAAAAAGAGAGGAGAAGGATATCAAATTTATTGAACATAATGGAACCAAGCCTCCTTCAGTTTTATATTTCTCGA CAGTGGCTTAATAAATTTAAGACCTTTGCCGAACCTGGCCCTATTTCAAATAATGACTTTCTTTGTATTCATGGAGGTGTTCCTCCAAGAAAAGCTGGTT ATATTGAAGACCTGGTTTTGATGCTGCCTCAGAACATTTGGGATAACCTATATAGCAGGTATGGTGGAGGACCAGCTGTCAACCATCTGTACATTTGTCA TACTTGCCAAATTGAGGCGGAGAAAATTGAAAAAAGAAGAAAAACTGAATTGGAAATTTTTATTCGGCTTAACAGAGCGTTCCAAAAAGAGGACTCTCCA GCTACTTTTTATTGCATCAGTATGCAGTGGTTTAGAGAATGGGAAAGTTTTGTGAAGGGTAAAGATGGAGATCCTCCAGGTCCTATTGACAATACTAAGA TTGCAGTCACTAAATGTGGTAATGTGATGCTTAGGCAAGGAGCAGATTCTGGCCAGATTTCTGAAGAAACATGGAATTTTCTGCAGTCTATTTATGGTGG AGGGCCTGAAGTTATCCTGCGACCTCCGGTTGTTCATGTTGATCCAGATATACTTCAAGCAGAAGAAAAAATTGAAGTAGAAACTCGGTCTTTGTAATTT TTAGGATGTAGAGAGTTCTAATGAGGAATCATTTTCATGTGCCCTGACATGTACACATGCGAAAACATTCCTAAAAGCGTGTTTATTTGCTTTATTTTTT TTCATCATTTATCCCATTTATTTCTTCTTAGTGGGCATTATGGAAGAATATATTAAAATGTGTAATATACCACAGGTTGGTATATTTAGTTTTAAATACT TACCATAAAGTCTTTCAGTGTAATTTTTTTTTGAGACAGAGTCTTGCTTTGTCACCCAGGCTGGAGTGCTGTGGTGTTACCTCAGCTCACTGCAGCCTCC ACCTCCTGGGTTCAAGCGATTCTCCTGCCTCAGCCTCTCGAGTAGCTGGGATTACAGGCACCTGCCACCATGCCCGGCTAATTTTTGTATTTTAGTAGAG ATGGGGTTTCACCATGTTGGCCAGGCTAGTCTCAAACTCCTGACCTCAGGTGATCCACCCACCTCGGCCTCCCAAAGTGCTGGGATTACAGGTGTGAGCC ACAGCGCCTGGCCCAGTGTAATATTTTTGAAAGAGGAGGGACAATTGTGAAATCAGTAGGTTATCTTTAATCTTTACACTACATGCAGATCCATAGTATC CTTTGTAGTGTTGTAAATACTTTTGCTTTGAAAACTTTTTCATTGTCCTAAATCACCCTGACTCTGACCAGTCTTTCAGTTCTCCAAAAGCCCAATTTAA TTGTATAGTTTTGTCATGGCTTCATATAATAAAGAGCCTATTTTAAGTTGAAAGTAGTAGTCAGAAAATTGTTAATTTCCTAAAGCTCAGGAAACTAGGG TGTCACTTTTTTTGCACTGCAGCATATACACTAACTAGCTTATTAAAATTTACAAAATGTCTTTTTGAATGTATCAAGGATATATTTAGTTTGAGTGGAA TTTGTCAGCAGATATCAGTAACTTATTGCCGCTTATATTGTACAATGTTAAACTTCAATTCCTGTAACCTGGTTAGTATTAATGTCAGTGACTAAAAAAC TTAGAGTTAGTTTTAGGGCACTTTTTATTTTGAGAGCATGAAGTGTGGAATGTGTCACTACGATTGTTGATAAAGCTGAGGCCACTTGCAACTTGATTTT TTAAATGAAATAGATAAAGTCTTTTTGAATAATATAGTATGCACTGCTATTTGCTTGATTATGTAATGTCAAAAGTTTAACTATATTCCAAGTACAAAAA CATACTGGATTACATTGAGGATGTTGAATAGCATTCATGATGGCTTTGTTTTGGTTTGGGGCAGCTGTCACCAGCTAAAGCAATGTTGTTAAAATTAGCT CAATAAAAATGTCTTTAAAATGTAAAA

refseq_USP33.F1 (forward primer)
refseq_USP33.R1 (reverse primer)
Single exon skipping

Frame 0

GDTGRSRGCCGKWPVQEADPPRAALRGRFPALL TRHCPSPRAEKEKRSLRRCGCRPLLVELAGPAGQ AVEVLPHFESLGKQEKIPNKMSAFRNHCPHLDS VGEITKEDLIQKSLGTCQDCKVQGPNLWACLEN RCSYVGCGESQVDHSTIHSQETKHYLTVNLTTLR VWCYACSKEVFLDRKLGTQPSLPHVRQPHQIQE NSVQDFKIPSNTTLKTPLVAVFDDLDIEADEED ELRARGLTGLKNIGNTCYMNAALQALSNCPPLTQ FFLDCGGLARTDKKPAICKSYLKLMTELWHKSR PGSVVPTTLFQGIKTVNPTFRGYSQQDAQEFLR CLMDLLHEELKEQVMEVEEDPQTITTEETMEEDK SQSDVDFQSCESCSNSDRAENENGSRCFSEDNN ETTMLIQDDENNSEMSKDWQKEKMCNKINKVNS EGEFDKDRDSISETVDLNNQETVKVQIHSRASEY ITDVHSNDLSTPQILPSNEGVNPRLSASPPKSG NLWPGLAPPHKKAQSASPKRKKQHKKYRSVISD IFDGTIISSVQCLTCDRVSVTLETFQDLSLPIPG KEDLAKLHSSSHPTSIVKAGSCGEAYAPQGWIA FFMEYVKRFVVSCVPSWFWGPVVTLQDCLAAFF ARDELKGDNMYSCEKCKKLRNGVKFCKVQNFPEI LCIHLKRFRHELMFSTKISTHVSFPLEGLDLQP FLAKDSPAQIVTYDLLSVICHHGTASSGHYIAY CRNNLNNLWYEFDDQSVTEVSESTVQNAEAYVLF YRKSSEEAQKERRRISNLLNIMEPSLLQFYISR QWLNKFKTFAEPGPISNNDFLCIHGGVPPRKAG YIEDLVLMLPQNIWDNLYSRYGGGPAVNHLYICH TCQIEAEKIEKRRKTELEIFIRLNRAFQKEDSP ATFYCISMQWFREWESFVKGKDGDPPGPIDNTK IAVTKCGNVMLRQGADSGQISEETWNFLQSIYGG GPEVILRPPVVHVDPDILQAEEKIEVETRSL*F LGCREF**GIIFMCPDMYTCENIPKSVFICFIF FHHLSHLFLLSGHYGRIY*NV*YTTGWYI*F*IL TIKSFSVIFF*DRVLLCHPGWSAVVLPQLTAAS TSWVQAILLPQPLE*LGLQAPATMPG*FLYFSR DGVSPCWPG*SQTPDLR*STHLGLPKCWDYRCEP QRLAQCNIFERGGTIVKSVGYL*SLHYMQIHSI LCSVVNTFALKTFSLS*ITLTLTSLSVLQKPNL IV*FCHGFI**RAYFKLKVVVRKLLIS*SSGN*G VTFFALQHIH*LAY*NLQNVFLNVSRIYLV*VE FVSRYQ*LIAAYIVQC*TSIPVTWLVLMSVTKK LRVSFRALFILRA*SVECVTTIVDKAEATCNLIF *MK*IKSF*II*YALLFA*LCNVKSLTIFQVQK HTGLH*GC*IAFMMALFWFGAAVTS*SNVVKIS SIKMSLKCK

Frame 1

ETPEGAAAAAGSGQFRRRTPRGQRCGAVFRPS* RDTAPLRELRRKRGACGGAAAGRCWSSWRVPRA RPWRCYLILKVLGNRKKFLTKCQLFEIIVHIWIQ LVK*QKKI*YKNPLVLVRIVKSKDQIFGHVWRI DVHMLAVVNHK*ITAPYILRRQSII*L*TLPLF EYGVMLAAKKYFWIGN*ELSLHCLM*DNLTKYKK TVSRILKYPVIQH*KLLWLPYLMIWI*KRMKKM NLGPEVLQV*KILEILVT*MQLCRLFLIAHL*H SFFLIVED*LEQIRNLPFVKVISN**QSCGIKAG QDLLCLLLCFKELKL*IQHFGGILSRMLKNSFD V*WICFMKN*KSKSWK*KKIRKP*PLRRQWKKT RASRM*IFSLVNLVATVIEQKMKMALDAFLKIIM KQQC*FRMMKTIQKCQRIGKKRRCAIRLIK*IL KANLIKIETLYLKQST*TTRKLSKCKYTAELQN ISLMSIRMTCLHHRSFHQMKVLIHVYRQALLNQA ICGQDWHHHTKKLSLHLQREKNSTRNTEVLFQT YLMEQSLVQCSV*LVTGCL*PSRPFKICPCQFL ARKTLLSCIHQVIQLL*SKQDHVAKHMLHKGG*L FSWNM*RGLLSHVSLAGFGVQ**PCKIVLLPSL PEMN*KVTICTVVKNAKS*EME*SFVKYKTFLR FCASTLKDSDMN*CFPPKSVPMFHFR*KAWIFSH FLLRIVQLKL*HMIFCQSFAIMELQVVDTI*PT AETI*IISGMNLMIRVSLKFQNLLYKMQKLTFF SIGRAAKRHKKRGEGYQIY*T*WNQASFSFIFLD SGLINLRPLPNLALFQIMTFFVFMEVFLQEKLV ILKTWF*CCLRTFGITYIAGMVEDQLSTICTFV ILAKLRRRKLKKEEKLNWKFLFGLTERSKKRTLQ LLFIASVCSGLENGKVL*RVKMEILQVLLTILR LQSLNVVM*CLGKEQILARFLKKHGIFCSLFMV EGLKLSCDLRLFMLIQIYFKQKKKLK*KLGLCNF *DVESSNEESFSCALTCTHAKTFLKACLFALFF FIIYPIYFFLVGIMEEYIKMCNIPQVGIFSFKY LP*SLSV*FFFETESCFVTQAGVLWCYLSSLQPP PPGFKRFSCLSLSSSWDYRHLPPCPANFCILVE MGFHHVGQASLKLLTSGDPPTSASQSAGITGVS HSAWPSVIFLKEEGQL*NQ*VIFNLYTTCRSIVS FVVL*ILLL*KLFHCPKSP*L*PVFQFSKSPI* LYSFVMASYNKEPILS*K**SENC*FPKAQETR VSLFLHCSIYTN*LIKIYKMSF*MYQGYI*FEWN LSADISNLLPLILYNVKLQFL*PG*Y*CQ*LKN LELVLGHFLF*EHEVWNVSLRLLIKLRPLAT*F FK*NR*SLFE*YSMHCYLLDYVMSKV*LYSKYKN ILDYIEDVE*HS*WLCFGLGQLSPAKAMLLKLA Q*KCL*NVK

Frame 2

RHRKEPRLLREVASSGGGPPEGSAAGPFSGPPD ATLPLSES*EGKEELAEVRLQAVVGRAGGSRGP GRGGVTSF*KSWETGKNS*QNVSFSKSLSTFGF SW*NNKRRFDTKIPWYLSGL*SPRTKSLGMSGE* MFICWLW*ITSRSQHHTFSGDKALSNCEPYHSS SMVLCLQQRSIFG*EIRNSAFIASCKTTSPNTR KQCPGF*NTQ*YNIKNSSGCRI**SGYRSG*RR* T*GQRSYRFEKYWKYLLHECSFAGSF*LPTFDT VFS*LWRTSSNR*ETCHL*KLSQTNDRAVA*KQ ARICCAYYSVSRN*NCKSNISGVFSAGCSRIPSM FNGFAS*RIERASHGSRRRSANHNH*GDNGRRQ EPVGCRFSVL*IL*QQ**SRK*KWL*MLF*R** *NNNVNSG**KQFRNVKGLAKREDVQ*D**SKF* RRI**R*RLYI*NSRLKQPGNCQSANTQQSFRI YH*CPFE*PVYTTDPSIK*RC*STFIGKPS*IR QFVARIGTTTQKSSVCISKEKKTAQEIQKCYFRH I*WNNH*FSAVSDL*QGVCNPRDLSRSVLANSW QGRPC*AAFIKSSNFYSQSRIMWRSICSTRVDS FFHGICEEVCCLMCP*LVLGSSSNLARLSCCLLC QR*TKR*QYVQL*KMQKVEKWSEVL*STKLS*D FVHPP*KIQT*TNVFHQNQYPCFISARRLGSSA ISC*G*SSSNCDI*SSVSHLPSWNCK*WTLYSLL PKQSK*SLV*I**SECH*SFRIYCTKCRSLRSF L*EEQRRGTKREEKDIKFIEHNGTKPPSVLYFS TVA**I*DLCRTWPYFK**LSLYSWRCSSKKSWL Y*RPGFDAASEHLG*PI*QVWWRTSCQPSVHLS YLPN*GGEN*KKKKN*IGNFYSA*QSVPKRGLS SYFLLHQYAVV*RMGKFCEG*RWRSSRSY*QY*D CSH*MW*CDA*ARSRFWPDF*RNMEFSAVYLWW RA*SYPATSGCSC*SRYTSSRRKN*SRNSVFVI FRM*RVLMRNHFHVP*HVHMRKHS*KRVYLLYFF SSFIPFISS*WALWKNILKCVIYHRLVYLVLNT YHKVFQCNFFLRQSLALSPRLECCGVTSAHCSL HLLGSSDSPASASRVAGITGTCHHARLIFVF**R WGFTMLARLVSNS*PQVIHPPRPPKVLGLQV*A TAPGPV*YF*KRRDNCEISRLSLIFTLHADP*Y PL*CCKYFCFENFFIVLNHPDSDQSFSSPKAQFN CIVLSWLHIIKSLF*VESSSQKIVNFLKLRKLG CHFFCTAAYTLTSLLKFTKCLFECIKDIFSLSG ICQQISVTYCRLYCTMLNFNSCNLVSINVSD*KT *S*F*GTFYFESMKCGMCHYDC**S*GHLQLDF LNEIDKVFLNNIVCTAICLIM*CQKFNYIPSTK TYWITLRMLNSIHDGFVLVWGSCHQLKQCC*N*L NKNVFKM*

Sequence NM_015017

GGAGACACCGGAAGGAGCCGCGGCTGCTGCGGGAAGTGGCCAGTTCAGGAGGCGGACCCCCCGAGGGCAGCGCTGCGGGGCCGTTTTCCGGCCCTCCTGA CGCGACACTGCCCCTCTCCGAGAGCTGAGAAGGAAAAGAGGAGCTTGCGGAGGTGCGGCTGCAGGCCGTTGTTGGTCGAGCTGGCGGGTCCCGCGGGCCA GGCCGTGGAGAGCTCCTGAAGGAAGCACTAAACATGGAAAGGAACAACCGGTACCAGCCACTGCAAAAACATGCCAAATTGTAAAGACCATCGATGTTAG GAAGAAACTGCCTCAACTAATGAGCAAAATAACCAGCTAACATCGTAATGACAGGATCAAATTCACACATAACGATATTAACCTTAAAGGTGTTACCTCA TTTTGAAAGTCTTGGGAAACAGGAAAAAATTCCTAACAAAATGTCAGCTTTTCGAAATCATTGTCCACATTTGGATTCAGTTGGTGAAATAACAAAAGAA GATTTGATACAAAAATCCCTTGGTACTTGTCAGGATTGTAAAGTCCAAGGACCAAATCTTTGGGCATGTCTGGAGAATAGATGTTCATATGTTGGCTGTG GTGAATCACAAGTAGATCACAGCACCATACATTCTCAGGAGACAAAGCATTATCTAACTGTGAACCTTACCACTCTTCGAGTATGGTGTTATGCTTGCAG CAAAGAAGTATTTTTGGATAGGAAATTAGGAACTCAGCCTTCATTGCCTCATGTAAGACAACCTCACCAAATACAAGAAAACAGTGTCCAGGATTTTAAA ATACCCAGTAATACAACATTAAAAACTCCTCTGGTTGCCGTATTTGATGATCTGGATATAGAAGCGGATGAAGAAGATGAACTTAGGGCCAGAGGTCTTA CAGGTTTGAAAAATATTGGAAATACTTGTTACATGAATGCAGCTTTGCAGGCTCTTTCTAATTGCCCACCTTTGACACAGTTTTTTCTTGATTGTGGAGG ACTAGCTCGAACAGATAAGAAACCTGCCATTTGTAAAAGTTATCTCAAACTAATGACAGAGCTGTGGCATAAAAGCAGGCCAGGATCTGTTGTGCCTACT ACTCTGTTTCAAGGAATTAAAACTGTAAATCCAACATTTCGGGGGTATTCTCAGCAGGATGCTCAAGAATTCCTTCGATGTTTAATGGATTTGCTTCATG AAGAATTGAAAGAGCAAGTCATGGAAGTAGAAGAAGATCCGCAAACCATAACCACTGAGGAGACAATGGAAGAAGACAAGAGCCAGTCGGATGTAGATTT TCAGTCTTGTGAATCTTGTAGCAACAGTGATAGAGCAGAAAATGAAAATGGCTCTAGATGCTTTTCTGAAGATAATAATGAAACAACAATGTTAATTCAG GATGATGAAAACAATTCAGAAATGTCAAAGGATTGGCAAAAAGAGAAGATGTGCAATAAGATTAATAAAGTAAATTCTGAAGGCGAATTTGATAAAGATA GAGACTCTATATCTGAAACAGTCGACTTAAACAACCAGGAAACTGTCAAAGTGCAAATACACAGCAGAGCTTCAGAATATATCACTGATGTCCATTCGAA TGACCTGTCTACACCACAGATCCTTCCATCAAATGAAGGTGTTAATCCACGTTTATCGGCAAGCCCTCCTAAATCAGGCAATTTGTGGCCAGGATTGGCA CCACCACACAAAAAAGCTCAGTCTGCATCTCCAAAGAGAAAAAAACAGCACAAGAAATACAGAAGTGTTATTTCAGACATATTTGATGGAACAATCATTA GTTCAGTGCAGTGTCTGACTTGTGACAGGGTGTCTGTAACCCTCGAGACCTTTCAAGATCTGTCCTTGCCAATTCCTGGCAAGGAAGACCTTGCTAAGCT GCATTCATCAAGTCATCCAACTTCTATAGTCAAAGCAGGATCATGTGGCGAAGCATATGCTCCACAAGGGTGGATAGCTTTTTTCATGGAATATGTGAAG AGGTTTGTTGTCTCATGTGTCCCTAGCTGGTTTTGGGGTCCAGTAGTAACCTTGCAAGATTGTCTTGCTGCCTTCTTTGCCAGAGATGAACTAAAAGGTG ACAATATGTACAGTTGTGAAAAATGCAAAAAGTTGAGAAATGGAGTGAAGTTTTGTAAAGTACAAAACTTTCCTGAGATTTTGTGCATCCACCTTAAAAG ATTCAGACATGAACTAATGTTTTCCACCAAAATCAGTACCCATGTTTCATTTCCGCTAGAAGGCTTGGATCTTCAGCCATTTCTTGCTAAGGATAGTCCA GCTCAAATTGTGACATATGATCTTCTGTCAGTCATTTGCCATCATGGAACTGCAAGTAGTGGACACTATATAGCCTACTGCCGAAACAATCTAAATAATC TCTGGTATGAATTTGATGATCAGAGTGTCACTGAAGTTTCAGAATCTACTGTACAAAATGCAGAAGCTTACGTTCTTTTCTATAGGAAGAGCAGCGAAGA GGCACAAAAAGAGAGGAGAAGGATATCAAATTTATTGAACATAATGGAACCAAGCCTCCTTCAGTTTTATATTTCTCGACAGTGGCTTAATAAATTTAAG ACCTTTGCCGAACCTGGCCCTATTTCAAATAATGACTTTCTTTGTATTCATGGAGGTGTTCCTCCAAGAAAAGCTGGTTATATTGAAGACCTGGTTTTGA TGCTGCCTCAGAACATTTGGGATAACCTATATAGCAGGTATGGTGGAGGACCAGCTGTCAACCATCTGTACATTTGTCATACTTGCCAAATTGAGGCGGA GAAAATTGAAAAAAGAAGAAAAACTGAATTGGAAATTTTTATTCGGCTTAACAGAGCGTTCCAAAAAGAGGACTCTCCAGCTACTTTTTATTGCATCAGT ATGCAGTGGTTTAGAGAATGGGAAAGTTTTGTGAAGGGTAAAGATGGAGATCCTCCAGGTCCTATTGACAATACTAAGATTGCAGTCACTAAATGTGGTA ATGTGATGCTTAGGCAAGGAGCAGATTCTGGCCAGATTTCTGAAGAAACATGGAATTTTCTGCAGTCTATTTATGGTGGAGGGCCTGAAGTTATCCTGCG ACCTCCGGTTGTTCATGTTGATCCAGATATACTTCAAGCAGAAGAAAAAATTGAAGTAGAAACTCGGTCTTTGTAATTTTTAGGATGTAGAGAGTTCTAA TGAGGAATCATTTTCATGTGCCCTGACATGTACACATGCGAAAACATTCCTAAAAGCGTGTTTATTTGCTTTATTTTTTTTCATCATTTATCCCATTTAT TTCTTCTTAGTGGGCATTATGGAAGAATATATTAAAATGTGTAATATACCACAGGTTGGTATATTTAGTTTTAAATACTTACCATAAAGTCTTTCAGTGT AATTTTTTTTTGAGACAGAGTCTTGCTTTGTCACCCAGGCTGGAGTGCTGTGGTGTTACCTCAGCTCACTGCAGCCTCCACCTCCTGGGTTCAAGCGATT CTCCTGCCTCAGCCTCTCGAGTAGCTGGGATTACAGGCACCTGCCACCATGCCCGGCTAATTTTTGTATTTTAGTAGAGATGGGGTTTCACCATGTTGGC CAGGCTAGTCTCAAACTCCTGACCTCAGGTGATCCACCCACCTCGGCCTCCCAAAGTGCTGGGATTACAGGTGTGAGCCACAGCGCCTGGCCCAGTGTAA TATTTTTGAAAGAGGAGGGACAATTGTGAAATCAGTAGGTTATCTTTAATCTTTACACTACATGCAGATCCATAGTATCCTTTGTAGTGTTGTAAATACT TTTGCTTTGAAAACTTTTTCATTGTCCTAAATCACCCTGACTCTGACCAGTCTTTCAGTTCTCCAAAAGCCCAATTTAATTGTATAGTTTTGTCATGGCT TCATATAATAAAGAGCCTATTTTAAGTTGAAAGTAGTAGTCAGAAAATTGTTAATTTCCTAAAGCTCAGGAAACTAGGGTGTCACTTTTTTTGCACTGCA GCATATACACTAACTAGCTTATTAAAATTTACAAAATGTCTTTTTGAATGTATCAAGGATATATTTAGTTTGAGTGGAATTTGTCAGCAGATATCAGTAA CTTATTGCCGCTTATATTGTACAATGTTAAACTTCAATTCCTGTAACCTGGTTAGTATTAATGTCAGTGACTAAAAAACTTAGAGTTAGTTTTAGGGCAC TTTTTATTTTGAGAGCATGAAGTGTGGAATGTGTCACTACGATTGTTGATAAAGCTGAGGCCACTTGCAACTTGATTTTTTAAATGAAATAGATAAAGTC TTTTTGAATAATATAGTATGCACTGCTATTTGCTTGATTATGTAATGTCAAAAGTTTAACTATATTCCAAGTACAAAAACATACTGGATTACATTGAGGA TGTTGAATAGCATTCATGATGGCTTTGTTTTGGTTTGGGGCAGCTGTCACCAGCTAAAGCAATGTTGTTAAAATTAGCTCAATAAAAATGTCTTTAAAAT GTAAAA

refseq_USP33.F1 (forward primer)
refseq_USP33.R1 (reverse primer)
Single exon skipping

Frame 0

GDTGRSRGCCGKWPVQEADPPRAALRGRFPALL TRHCPSPRAEKEKRSLRRCGCRPLLVELAGPAGQ AVESS*RKH*TWKGTTGTSHCKNMPNCKDHRC* EETASTNEQNNQLTS**QDQIHT*RY*P*RCYL ILKVLGNRKKFLTKCQLFEIIVHIWIQLVK*QKK I*YKNPLVLVRIVKSKDQIFGHVWRIDVHMLAV VNHK*ITAPYILRRQSII*L*TLPLFEYGVMLA AKKYFWIGN*ELSLHCLM*DNLTKYKKTVSRILK YPVIQH*KLLWLPYLMIWI*KRMKKMNLGPEVL QV*KILEILVT*MQLCRLFLIAHL*HSFFLIVE D*LEQIRNLPFVKVISN**QSCGIKAGQDLLCLL LCFKELKL*IQHFGGILSRMLKNSFDV*WICFM KN*KSKSWK*KKIRKP*PLRRQWKKTRASRM*I FSLVNLVATVIEQKMKMALDAFLKIIMKQQC*FR MMKTIQKCQRIGKKRRCAIRLIK*ILKANLIKI ETLYLKQST*TTRKLSKCKYTAELQNISLMSIR MTCLHHRSFHQMKVLIHVYRQALLNQAICGQDWH HHTKKLSLHLQREKNSTRNTEVLFQTYLMEQSL VQCSV*LVTGCL*PSRPFKICPCQFLARKTLLS CIHQVIQLL*SKQDHVAKHMLHKGG*LFSWNM*R GLLSHVSLAGFGVQ**PCKIVLLPSLPEMN*KV TICTVVKNAKS*EME*SFVKYKTFLRFCASTLK DSDMN*CFPPKSVPMFHFR*KAWIFSHFLLRIVQ LKL*HMIFCQSFAIMELQVVDTI*PTAETI*II SGMNLMIRVSLKFQNLLYKMQKLTFFSIGRAAK RHKKRGEGYQIY*T*WNQASFSFIFLDSGLINLR PLPNLALFQIMTFFVFMEVFLQEKLVILKTWF* CCLRTFGITYIAGMVEDQLSTICTFVILAKLRR RKLKKEEKLNWKFLFGLTERSKKRTLQLLFIASV CSGLENGKVL*RVKMEILQVLLTILRLQSLNVV M*CLGKEQILARFLKKHGIFCSLFMVEGLKLSC DLRLFMLIQIYFKQKKKLK*KLGLCNF*DVESSN EESFSCALTCTHAKTFLKACLFALFFFIIYPIY FFLVGIMEEYIKMCNIPQVGIFSFKYLP*SLSV *FFFETESCFVTQAGVLWCYLSSLQPPPPGFKRF SCLSLSSSWDYRHLPPCPANFCILVEMGFHHVG QASLKLLTSGDPPTSASQSAGITGVSHSAWPSV IFLKEEGQL*NQ*VIFNLYTTCRSIVSFVVL*IL LL*KLFHCPKSP*L*PVFQFSKSPI*LYSFVMA SYNKEPILS*K**SENC*FPKAQETRVSLFLHC SIYTN*LIKIYKMSF*MYQGYI*FEWNLSADISN LLPLILYNVKLQFL*PG*Y*CQ*LKNLELVLGH FLF*EHEVWNVSLRLLIKLRPLAT*FFK*NR*S LFE*YSMHCYLLDYVMSKV*LYSKYKNILDYIED VE*HS*WLCFGLGQLSPAKAMLLKLAQ*KCL*N VK

Frame 1

ETPEGAAAAAGSGQFRRRTPRGQRCGAVFRPS* RDTAPLRELRRKRGACGGAAAGRCWSSWRVPRA RPWRAPEGSTKHGKEQPVPATAKTCQIVKTIDVR KKLPQLMSKITS*HRNDRIKFTHNDINLKGVTS F*KSWETGKNS*QNVSFSKSLSTFGFSW*NNKR RFDTKIPWYLSGL*SPRTKSLGMSGE*MFICWLW *ITSRSQHHTFSGDKALSNCEPYHSSSMVLCLQ QRSIFG*EIRNSAFIASCKTTSPNTRKQCPGF* NTQ*YNIKNSSGCRI**SGYRSG*RR*T*GQRSY RFEKYWKYLLHECSFAGSF*LPTFDTVFS*LWR TSSNR*ETCHL*KLSQTNDRAVA*KQARICCAY YSVSRN*NCKSNISGVFSAGCSRIPSMFNGFAS* RIERASHGSRRRSANHNH*GDNGRRQEPVGCRF SVL*IL*QQ**SRK*KWL*MLF*R***NNNVNS G**KQFRNVKGLAKREDVQ*D**SKF*RRI**R* RLYI*NSRLKQPGNCQSANTQQSFRIYH*CPFE *PVYTTDPSIK*RC*STFIGKPS*IRQFVARIG TTTQKSSVCISKEKKTAQEIQKCYFRHI*WNNH* FSAVSDL*QGVCNPRDLSRSVLANSWQGRPC*A AFIKSSNFYSQSRIMWRSICSTRVDSFFHGICE EVCCLMCP*LVLGSSSNLARLSCCLLCQR*TKR* QYVQL*KMQKVEKWSEVL*STKLS*DFVHPP*K IQT*TNVFHQNQYPCFISARRLGSSAISC*G*S SSNCDI*SSVSHLPSWNCK*WTLYSLLPKQSK*S LV*I**SECH*SFRIYCTKCRSLRSFL*EEQRR GTKREEKDIKFIEHNGTKPPSVLYFSTVA**I* DLCRTWPYFK**LSLYSWRCSSKKSWLY*RPGFD AASEHLG*PI*QVWWRTSCQPSVHLSYLPN*GG EN*KKKKN*IGNFYSA*QSVPKRGLSSYFLLHQ YAVV*RMGKFCEG*RWRSSRSY*QY*DCSH*MW* CDA*ARSRFWPDF*RNMEFSAVYLWWRA*SYPA TSGCSC*SRYTSSRRKN*SRNSVFVIFRM*RVL MRNHFHVP*HVHMRKHS*KRVYLLYFFSSFIPFI SS*WALWKNILKCVIYHRLVYLVLNTYHKVFQC NFFLRQSLALSPRLECCGVTSAHCSLHLLGSSD SPASASRVAGITGTCHHARLIFVF**RWGFTMLA RLVSNS*PQVIHPPRPPKVLGLQV*ATAPGPV* YF*KRRDNCEISRLSLIFTLHADP*YPL*CCKY FCFENFFIVLNHPDSDQSFSSPKAQFNCIVLSWL HIIKSLF*VESSSQKIVNFLKLRKLGCHFFCTA AYTLTSLLKFTKCLFECIKDIFSLSGICQQISV TYCRLYCTMLNFNSCNLVSINVSD*KT*S*F*GT FYFESMKCGMCHYDC**S*GHLQLDFLNEIDKV FLNNIVCTAICLIM*CQKFNYIPSTKTYWITLR MLNSIHDGFVLVWGSCHQLKQCC*N*LNKNVFKM *

Frame 2

RHRKEPRLLREVASSGGGPPEGSAAGPFSGPPD ATLPLSES*EGKEELAEVRLQAVVGRAGGSRGP GRGELLKEALNMERNNRYQPLQKHAKL*RPSML GRNCLN**AK*PANIVMTGSNSHITILTLKVLPH FESLGKQEKIPNKMSAFRNHCPHLDSVGEITKE DLIQKSLGTCQDCKVQGPNLWACLENRCSYVGC GESQVDHSTIHSQETKHYLTVNLTTLRVWCYACS KEVFLDRKLGTQPSLPHVRQPHQIQENSVQDFK IPSNTTLKTPLVAVFDDLDIEADEEDELRARGL TGLKNIGNTCYMNAALQALSNCPPLTQFFLDCGG LARTDKKPAICKSYLKLMTELWHKSRPGSVVPT TLFQGIKTVNPTFRGYSQQDAQEFLRCLMDLLH EELKEQVMEVEEDPQTITTEETMEEDKSQSDVDF QSCESCSNSDRAENENGSRCFSEDNNETTMLIQ DDENNSEMSKDWQKEKMCNKINKVNSEGEFDKD RDSISETVDLNNQETVKVQIHSRASEYITDVHSN DLSTPQILPSNEGVNPRLSASPPKSGNLWPGLA PPHKKAQSASPKRKKQHKKYRSVISDIFDGTII SSVQCLTCDRVSVTLETFQDLSLPIPGKEDLAKL HSSSHPTSIVKAGSCGEAYAPQGWIAFFMEYVK RFVVSCVPSWFWGPVVTLQDCLAAFFARDELKG DNMYSCEKCKKLRNGVKFCKVQNFPEILCIHLKR FRHELMFSTKISTHVSFPLEGLDLQPFLAKDSP AQIVTYDLLSVICHHGTASSGHYIAYCRNNLNN LWYEFDDQSVTEVSESTVQNAEAYVLFYRKSSEE AQKERRRISNLLNIMEPSLLQFYISRQWLNKFK TFAEPGPISNNDFLCIHGGVPPRKAGYIEDLVL MLPQNIWDNLYSRYGGGPAVNHLYICHTCQIEAE KIEKRRKTELEIFIRLNRAFQKEDSPATFYCIS MQWFREWESFVKGKDGDPPGPIDNTKIAVTKCG NVMLRQGADSGQISEETWNFLQSIYGGGPEVILR PPVVHVDPDILQAEEKIEVETRSL*FLGCREF* *GIIFMCPDMYTCENIPKSVFICFIFFHHLSHL FLLSGHYGRIY*NV*YTTGWYI*F*ILTIKSFSV IFF*DRVLLCHPGWSAVVLPQLTAASTSWVQAI LLPQPLE*LGLQAPATMPG*FLYFSRDGVSPCW PG*SQTPDLR*STHLGLPKCWDYRCEPQRLAQCN IFERGGTIVKSVGYL*SLHYMQIHSILCSVVNT FALKTFSLS*ITLTLTSLSVLQKPNLIV*FCHG FI**RAYFKLKVVVRKLLIS*SSGN*GVTFFALQ HIH*LAY*NLQNVFLNVSRIYLV*VEFVSRYQ* LIAAYIVQC*TSIPVTWLVLMSVTKKLRVSFRA LFILRA*SVECVTTIVDKAEATCNLIF*MK*IKS F*II*YALLFA*LCNVKSLTIFQVQKHTGLH*G C*IAFMMALFWFGAAVTS*SNVVKISSIKMSLK CK

Sequence NM_201624

Frame 0

Frame 1

Frame 2

Sequence NM_015017

Frame 0

Frame 1

Frame 2

Sequence NM_201626

Frame 0

Frame 1

Frame 2