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Sequences

Sequence NM_001010938

GGAGAGGAGGAACGGAGCTGGGACGGGCTGCAGGAGGGAATGGCCGCAGGCCGGCAGGAGGAGGGGCGCGACCACCCGGCCGCCGGCCGATGGCGGATGA
GGCTATGGGGGCTGAGACGCAGTAGCGGCCCTGCACTTCTGGAGGATGCTCGAGGCCCGGCCCCCCAGGACGCAGGGCAGTGACGCTGCCGGTGCCGCTG
CGGGGCGGGGGCTGCGGGCGCTGCTTCTCTCCCTGACCGCAGCCGCTGGGATTTGGGGCTCCATGGGGGAGAGATCTGCTTACCAGCGCCTGGCTGGGGG
CGAGGAGGGACCGCAGAGGCTGGGAGGCGGCAGAATGCAGCCAGAGGAGGGCACAGGCTGGCTGCTGGAGCTGCTGTCCGAGGTGCAGCTGCAACAGTAC
TTCCTGCGGCTCCGAGATGACCTCAACGTCACCCGCCTGTCCCACTTTGAGTACGTCAAGAATGAGGACCTGGAGAAGATCGGCATGGGTCGGCCTGGCC
AGCGGCGGCTGTGGGAGGCTGTGAAGAGGAGGAAGGCCTTGTGCAAACGCAAGTCGTGGATGAGTAAGGTGTTCAGTGGAAAGCGACTGGAGGCTGAGTT
CCCACCTCATCACTCTCAGAGCACCTTCCGGAAGACCTCGCCCGCCCCTGGGGGCCCAGCAGGGGAGGGGCCCCTGCAGAGCCTCACCTGCCTCATTGGG
GAGAAGGACCTGCGCCTCCTGGAGAAGCTGGGTGATGGTTCCTTTGGCGTGGTGCGCAGGGGCGAGTGGGACGCGCCCTCAGGGAAGACGGTGAGTGTGG
CTGTGAAGTGCCTGAAGCCCGATGTCCTGAGCCAGCCAGAAGCCATGGACGACTTCATCCGGGAGGTCAATGCCATGCACTCGCTCGACCACCGAAACCT
CATCCGCCTCTACGGGGTGGTGCTCACGCCGCCCATGAAGATGGTGACAGAGCTGGCACCTCTGGGATCGTTGTTGGACCGGCTACGTAAGCACCAGGGC
CACTTCCTCCTGGGGACTCTGAGCCGCTACGCTGTGCAGGTGGCTGAGGGCATGGGCTACCTGGAGTCCAAGCGCTTTATTCACCGTGACCTGGCTGCCC
GCAATCTGCTGTTGGCTACCCGCGACCTGGTCAAGATCGGGGACTTTGGGCTGATGCGAGCACTACCTCAGAATGACGACCATTACGTCATGCAGGAACA
TCGCAAGGTGCCCTTCGCCTGGTGTGCCCCCGAGAGCCTGAAGACACGCACCTTCTCCCATGCCAGCGACACCTGGATGTTCGGGGTGACACTGTGGGAA
ATGTTCACCTACGGCCAGGAGCCCTGGATCGGCCTCAACGGCAGTCAGATCCTGCATAAGATCGACAAGGAGGGGGAGCGGCTGCCCCGGCCCGAGGACT
GTCCCCAGGACATCTACAACGTCATGGTCCAGTGCTGGGCTCACAAGCCAGAGGACAGACCCACGTTTGTGGCCCTGCGGGACTTCCTGCTGGAGGCCCA
GCCCACAGACATGCGGGCCCTTCAGGACTTTGAGGAACCGGACAAGCTGCACATCCAGATGAATGATGTCATCACCGTCATCGAGGGAAGGGCCGAGAAC
TACTGGTGGCGTGGCCAGAACACACGGACGCTGTGTGTGGGGCCCTTCCCTCGCAACGTGGTGACCTCCGTGGCCGGCCTGTCGGCCCAGGACATCAGCC
AGCCCCTGCAGAACAGCTTCATCCACACAGGGCATGGCGACAGTGACCCCCGCCACTGCTGGGGCTTCCCGGACAGGATTGACGAACTGTATCTGGGAAA
CCCCATGGACCCCCCCGACCTCCTGAGCGTGGAACTGAGCACCTCCCGGCCCCCCCAGCATCTAGGAGGGGTGAAAAGGGAGCCTCCACCTCGCCCACCT
CAGCCTGCCTTCTTCACTCAGAAACCAACCTATGACCCTGTGAGCGAGGACCAAGACCCCTTGTCCAGCGACTTCAAGAGGCTGGGCCTGCGGAAGCCAG
GCCTGCCCCGAGGGCTGTGGCTGGCGAAGCCCTCGGCGCGGGTGCCGGGCACCAAGGCCAGCCGAGGCAGCGGGGCTGAGGTCACGCTCATCGACTTCGG
TGAGGAGCCCGTGGTCCCGGCCCTACGGCCCTGCGCGCCCTCCCTGGCGCAGCTGGCCATGGACGCCTGCTCCCTGCTGGACGAGACCCCGCCTCAGAGC
CCCACGCGGGCACTGCCCCGGCCCCTGCACCCCACGCCTGTGGTGGACTGGGACGCACGCCCGCTGCCCCCCCCGCCCGCCTATGACGACGTGGCCCAGG
ATGAGGATGACTTTGAGATCTGCTCCATCAACAGCACCCTCGTGGGCGCGGGGGTCCCTGCCGGGCCCAGCCAGGGCCAGACCAACTACGCCTTTGTGCC
TGAGCAGGCGCGGCCGCCCCCTCCCCTGGAGGACAACCTGTTCCTCCCGCCCCAGGGTGGGGGCAAGCCGCCCAGCTCCGCACAGACCGCAGAGATCTTC
CAGGCGCTACAGCAGGAGTGCATGAGGCAACTGCAGGCTCCGGCCGGCTCCCCGGCCCCCTCTCCCAGCCCGGGGGGTGACGACAAGCCCCAGGTGCCTC
CTCGGGTACCCATCCCCCCTCGGCCCACGCGCCCACACGTCCAGCTGTCTCCAGCCCCCCCGGGCGAGGAGGAGACCAGCCAGTGGCCTGGACCTGCTTC
CCCTCCCCGGGTGCCTCCGCGGGAGCCCCTGTCCCCTCAAGGCTCGAGGACACCCAGCCCCCTGGTACCACCTGGCAGCTCCCCGCTGCCACCCCGGCTC
TCAAGCTCACCTGGGAAGACCATGCCCACCACCCAGAGCTTTGCCTCAGACCCCAAGTACGCCACCCCCCAGGTGATCCAGGCCCCTGGCCCGCGGGCTG
GTCCCTGCATCCTGCCCATCGTCCGGGATGGCAAGAAGGTCAGCAGCACCCACTATTACTTGCTGCCCGAGCGACCATCCTACCTGGAGCGCTACCAGCG
CTTCCTGCGTGAGGCCCAGAGCCCCGAGGAGCCTACCCCCCTGCCTGTGCCTCTGCTGCTGCCCCCACCCAGCACCCCAGCCCCCGCCGCCCCCACGGCC
ACCGTGCGGCCGATGCCCCAGGCTGCCTTGGACCCCAAGGCCAACTTCTCCACCAACAACAGCAACCCAGGGGCCCGGCCACCACCCCCGAGGGCCACTG
CTCGGCTGCCACAGAGGGGCTGCCCTGGCGATGGGCCAGAGGCGGGCCGGCCAGCAGACAAGATCCAGATGGTGGAGCAGCTCTTCGGGCTGGGTCTGCG
GCCCAGAGGGGAGTGCCACAAAGTGCTGGAGATGTTCGACTGGAACCTGGAGCAGGCCGGCTGCCACCTTCTGGGCTCCTGGGGCCCTGCCCACCACAAG
CGCTGAGATGCGTCTGGAGAGCCAGAGGGCCTGCCTGAAGGAATCACCTGAGCCTGTCCGTCCACCAGGAGTGGGGAGATGCCCCCATCCAGTCCTGGAG
GACCCGCTGCTCCTGCTGCTCCCGGGGATGGAGCAAGGCCAAGGCTGCGGGAGGCTGGGAGCCCTGCCCTGCCCATCCCTCCTGCACCAGCGCTGTCCCT
GCACACTTTGGTTCAGTCCCGGTGCCCCTGCCAAGATGTGGAAGGGGCCGGGTGAAGACAGGCTTGAGGGCTGCCCCAGCAGGCTCTGGGTATGACCTGC
CTCTGGCCCTGGTCCTGGGCGGGGCCTGTGGGTGGAGTAGTACCCCCAGGCCCTGCCCTGGGTGACAGACTGGGAGGAAACCAGGCTGGACCTGGGCAGG
CGGGATGTGTTGGCCACAGGGAGAGGCGGACCGGCACCCGGTGGGACCTCCTAGGACTGGGCCTTCTTCCAGGGGGCCCCTGGCAGCAGCTGGGGTGTCG
GGCAGAATGTGACTTGTGGCCTTACCATGGACTTGAATGGGACTTGGCTGGCCTCAGGATCTTGTGCCTGGAAATAGCCTGAGGTGGCTCAGGAAGCGGA
GAAAGGGTGCCAGACCATTCTCTGGCGGGGACCAGGGCCCAAGGCCCCAGGGCTGGAAGGAGACCAAGGGGCAGCCGCCCTGGAGGGACATCAGTGCTTC
CTCTTCCACCCAATTCCCCCACGCGGTTCCATGTTTTCCCACCAGCCTGTTGGCGAAGTTGCTGCTCCGGCATTCAGTACCTGCTTCTTCCAGAGAAATA
AAGTTAGTTTCTATTTTATGTT
  • refseq_TNK2.F1 (forward primer)
  • refseq_TNK2.R1 (reverse primer)
  • Single exon skipping

Frame 0

GEEERSWDGLQEGMAAGRQEEGRDHPAAGRWRM
RLWGLRRSSGPALLEDARGPAPQDAGQ*RCRCRC
GAGAAGAASLPDRSRWDLGLHGGEICLPAPGWG
RGGTAEAGRRQNAARGGHRLAAGAAVRGAAATV
LPAAPR*PQRHPPVPL*VRQE*GPGEDRHGSAWP
AAAVGGCEEEEGLVQTQVVDE*GVQWKATGG*V
PTSSLSEHLPEDLARPWGPSRGGAPAEPHLPHW
GEGPAPPGEAG*WFLWRGAQGRVGRALREDGECG
CEVPEARCPEPARSHGRLHPGGQCHALARPPKP
HPPLRGGAHAAHEDGDRAGTSGIVVGPAT*APG
PLPPGDSEPLRCAGG*GHGLPGVQALYSP*PGCP
QSAVGYPRPGQDRGLWADASTTSE*RPLRHAGT
SQGALRLVCPREPEDTHLLPCQRHLDVRGDTVG
NVHLRPGALDRPQRQSDPA*DRQGGGAAAPARGL
SPGHLQRHGPVLGSQARGQTHVCGPAGLPAGGP
AHRHAGPSGL*GTGQAAHPDE*CHHRHRGKGRE
LLVAWPEHTDAVCGALPSQRGDLRGRPVGPGHQP
APAEQLHPHRAWRQ*PPPLLGLPGQD*RTVSGK
PHGPPRPPERGTEHLPAPPASRRGEKGASTSPT
SACLLHSETNL*PCERGPRPLVQRLQEAGPAEAR
PAPRAVAGEALGAGAGHQGQPRQRG*GHAHRLR
*GARGPGPTALRALPGAAGHGRLLPAGRDPASE
PHAGTAPAPAPHACGGLGRTPAAPPARL*RRGPG
*G*L*DLLHQQHPRGRGGPCRAQPGPDQLRLCA
*AGAAAPSPGGQPVPPAPGWGQAAQLRTDRRDL
PGATAGVHEATAGSGRLPGPLSQPGG*RQAPGAS
SGTHPPSAHAPTRPAVSSPPGRGGDQPVAWTCF
PSPGASAGAPVPSRLEDTQPPGTTWQLPAATPA
LKLTWEDHAHHPELCLRPQVRHPPGDPGPWPAGW
SLHPAHRPGWQEGQQHPLLLAARATILPGALPA
LPA*GPEPRGAYPPACASAAAPTQHPSPRRPHG
HRAADAPGCLGPQGQLLHQQQQPRGPATTPEGHC
SAATEGLPWRWARGGPASRQDPDGGAALRAGSA
AQRGVPQSAGDVRLEPGAGRLPPSGLLGPCPPQ
ALRCVWRARGPA*RNHLSLSVHQEWGDAPIQSWR
TRCSCCSRGWSKAKAAGGWEPCPAHPSCTSAVP
AHFGSVPVPLPRCGRGRVKTGLRAAPAGSGYDL
PLALVLGGACGWSSTPRPCPG*QTGRKPGWTWAG
GMCWPQGEADRHPVGPPRTGPSSRGPLAAAGVS
GRM*LVALPWT*MGLGWPQDLVPGNSLRWLRKR
RKGARPFSGGDQGPRPQGWKETKGQPPWRDISAS
SSTQFPHAVPCFPTSLLAKLLLRHSVPASSREI
KLVSILC

Frame 1

ERRNGAGTGCRREWPQAGRRRGATTRPPADGG*
GYGG*DAVAALHFWRMLEARPPRTQGSDAAGAA
AGRGLRALLLSLTAAAGIWGSMGERSAYQRLAGG
EEGPQRLGGGRMQPEEGTGWLLELLSEVQLQQY
FLRLRDDLNVTRLSHFEYVKNEDLEKIGMGRPG
QRRLWEAVKRRKALCKRKSWMSKVFSGKRLEAEF
PPHHSQSTFRKTSPAPGGPAGEGPLQSLTCLIG
EKDLRLLEKLGDGSFGVVRRGEWDAPSGKTVSV
AVKCLKPDVLSQPEAMDDFIREVNAMHSLDHRNL
IRLYGVVLTPPMKMVTELAPLGSLLDRLRKHQG
HFLLGTLSRYAVQVAEGMGYLESKRFIHRDLAA
RNLLLATRDLVKIGDFGLMRALPQNDDHYVMQEH
RKVPFAWCAPESLKTRTFSHASDTWMFGVTLWE
MFTYGQEPWIGLNGSQILHKIDKEGERLPRPED
CPQDIYNVMVQCWAHKPEDRPTFVALRDFLLEAQ
PTDMRALQDFEEPDKLHIQMNDVITVIEGRAEN
YWWRGQNTRTLCVGPFPRNVVTSVAGLSAQDIS
QPLQNSFIHTGHGDSDPRHCWGFPDRIDELYLGN
PMDPPDLLSVELSTSRPPQHLGGVKREPPPRPP
QPAFFTQKPTYDPVSEDQDPLSSDFKRLGLRKP
GLPRGLWLAKPSARVPGTKASRGSGAEVTLIDFG
EEPVVPALRPCAPSLAQLAMDACSLLDETPPQS
PTRALPRPLHPTPVVDWDARPLPPPPAYDDVAQ
DEDDFEICSINSTLVGAGVPAGPSQGQTNYAFVP
EQARPPPPLEDNLFLPPQGGGKPPSSAQTAEIF
QALQQECMRQLQAPAGSPAPSPSPGGDDKPQVP
PRVPIPPRPTRPHVQLSPAPPGEEETSQWPGPAS
PPRVPPREPLSPQGSRTPSPLVPPGSSPLPPRL
SSSPGKTMPTTQSFASDPKYATPQVIQAPGPRA
GPCILPIVRDGKKVSSTHYYLLPERPSYLERYQR
FLREAQSPEEPTPLPVPLLLPPPSTPAPAAPTA
TVRPMPQAALDPKANFSTNNSNPGARPPPPRAT
ARLPQRGCPGDGPEAGRPADKIQMVEQLFGLGLR
PRGECHKVLEMFDWNLEQAGCHLLGSWGPAHHK
R*DASGEPEGLPEGIT*ACPSTRSGEMPPSSPG
GPAAPAAPGDGARPRLREAGSPALPIPPAPALSL
HTLVQSRCPCQDVEGAG*RQA*GLPQQALGMTC
LWPWSWAGPVGGVVPPGPALGDRLGGNQAGPGQ
AGCVGHRERRTGTRWDLLGLGLLPGGPWQQLGCR
AECDLWPYHGLEWDLAGLRILCLEIA*GGSGSG
ERVPDHSLAGTRAQGPRAGRRPRGSRPGGTSVL
PLPPNSPTRFHVFPPACWRSCCSGIQYLLLPEK*
S*FLFYV

Frame 2

RGGTELGRAAGGNGRRPAGGGARPPGRRPMADE
AMGAETQ*RPCTSGGCSRPGPPGRRAVTLPVPL
RGGGCGRCFSP*PQPLGFGAPWGRDLLTSAWLG
ARRDRRGWEAAECSQRRAQAGCWSCCPRCSCNST
SCGSEMTSTSPACPTLSTSRMRTWRRSAWVGLA
SGGCGRL*RGGRPCANASRG*VRCSVESDWRLS
SHLITLRAPSGRPRPPLGAQQGRGPCRASPASLG
RRTCASWRSWVMVPLAWCAGASGTRPQGRR*VW
L*SA*SPMS*ASQKPWTTSSGRSMPCTRSTTET
SSASTGWCSRRP*RW*QSWHLWDRCWTGYVSTRA
TSSWGL*AATLCRWLRAWATWSPSALFTVTWLP
AICCWLPATWSRSGTLG*CEHYLRMTTITSCRN
IARCPSPGVPPRA*RHAPSPMPATPGCSG*HCGK
CSPTARSPGSASTAVRSCIRSTRRGSGCPGPRT
VPRTSTTSWSSAGLTSQRTDPRLWPCGTSCWRP
SPQTCGPFRTLRNRTSCTSR*MMSSPSSREGPRT
TGGVARTHGRCVWGPSLATW*PPWPACRPRTSA
SPCRTASSTQGMATVTPATAGASRTGLTNCIWE
TPWTPPTS*AWN*APPGPPSI*EG*KGSLHLAHL
SLPSSLRNQPMTL*ARTKTPCPATSRGWACGSQ
ACPEGCGWRSPRRGCRAPRPAEAAGLRSRSSTS
VRSPWSRPYGPARPPWRSWPWTPAPCWTRPRLRA
PRGHCPGPCTPRLWWTGTHARCPPRPPMTTWPR
MRMTLRSAPSTAPSWARGSLPGPARARPTTPLC
LSRRGRPLPWRTTCSSRPRVGASRPAPHRPQRSS
RRYSRSA*GNCRLRPAPRPPLPARGVTTSPRCL
LGYPSPLGPRAHTSSCLQPPRARRRPASGLDLL
PLPGCLRGSPCPLKARGHPAPWYHLAAPRCHPGS
QAHLGRPCPPPRALPQTPSTPPPR*SRPLARGL
VPASCPSSGMARRSAAPTITCCPSDHPTWSATS
ASCVRPRAPRSLPPCLCLCCCPHPAPQPPPPPRP
PCGRCPRLPWTPRPTSPPTTATQGPGHHPRGPL
LGCHRGAALAMGQRRAGQQTRSRWWSSSSGWVC
GPEGSATKCWRCSTGTWSRPAATFWAPGALPTTS
AEMRLESQRACLKESPEPVRPPGVGRCPHPVLE
DPLLLLLPGMEQGQGCGRLGALPCPSLLHQRCP
CTLWFSPGAPAKMWKGPGEDRLEGCPSRLWV*PA
SGPGPGRGLWVE*YPQALPWVTDWEETRLDLGR
RDVLATGRGGPAPGGTS*DWAFFQGAPGSSWGV
GQNVTCGLTMDLNGTWLASGSCAWK*PEVAQEAE
KGCQTILWRGPGPKAPGLEGDQGAAALEGHQCF
LFHPIPPRGSMFSHQPVGEVAAPAFSTCFFQRN
KVSFYFM

Sequence NM_005781

GGGCCGGAAAGTTCCGGCGAACTGGGGGAGCGGCAGTGGGAGAGCGTCGGGCGGGAGGCGGTGGTCCTGGGAGCGGCCCCGGCGCCCCGAAGCCGCCCCG
AGCTGGTGGGGAGGGCCCGGCCGGTGGGGCCCCGGGGGCCTGCGGGGGAGGGGCGCCTGGGGGTGCAAGAGGCGAATTGGCTGCCCGGGGTGCAGGAGGG
ACAGGTGGTGCGGGCTGTCCAGGAAACCTACCTGAACGGGAAGGAGCCCCAGGAGGGAGAGGGACCCGGCGAGGGGCTCAGGACCCGGAGGCGCCGGCGG
AGGAGGAGGTGGTGACTGGCAGGCCCGGGCCCCACGGTACCTCCGGGGCTGAAGGGGACGCAGGATGTAGGGGCATGGGGAGTCGGGCGCAGAAGAGTGC
GGGGAACCGCTGAGCTCTGGGAGCCACTGCCTGAGGGCAGGCCGCGGCCGGCGGGAACCTCTTCTGCCGTCTCAGCCTGGGCGTCGCTGAAGCTGTGTCT
GCGGGGAGGCAGCGGAAGGCGGCAGAGGCTGGGAGGCGGCAGAATGCAGCCAGAGGAGGGCACAGGCTGGCTGCTGGAGCTGCTGTCCGAGGTGCAGCTG
CAACAGTACTTCCTGCGGCTCCGAGATGACCTCAACGTCACCCGCCTGTCCCACTTTGAGTACGTCAAGAATGAGGACCTGGAGAAGATCGGCATGGGTC
GGCCTGGCCAGCGGCGGCTGTGGGAGGCTGTGAAGAGGAGGAAGGCCTTGTGCAAACGCAAGTCGTGGATGAGTAAGGTGTTCAGTGGAAAGCGACTGGA
GGCTGAGTTCCCACCTCATCACTCTCAGAGCACCTTCCGGAAGACCTCGCCCGCCCCTGGGGGCCCAGCAGGGGAGGGGCCCCTGCAGAGCCTCACCTGC
CTCATTGGGGAGAAGGACCTGCGCCTCCTGGAGAAGCTGGGTGATGGTTCCTTTGGCGTGGTGCGCAGGGGCGAGTGGGACGCGCCCTCAGGGAAGACGG
TGAGTGTGGCTGTGAAGTGCCTGAAGCCCGATGTCCTGAGCCAGCCAGAAGCCATGGACGACTTCATCCGGGAGGTCAATGCCATGCACTCGCTCGACCA
CCGAAACCTCATCCGCCTCTACGGGGTGGTGCTCACGCCGCCCATGAAGATGGTGACAGAGCTGGCACCTCTGGGATCGTTGTTGGACCGGCTACGTAAG
CACCAGGGCCACTTCCTCCTGGGGACTCTGAGCCGCTACGCTGTGCAGGTGGCTGAGGGCATGGGCTACCTGGAGTCCAAGCGCTTTATTCACCGTGACC
TGGCTGCCCGCAATCTGCTGTTGGCTACCCGCGACCTGGTCAAGATCGGGGACTTTGGGCTGATGCGAGCACTACCTCAGAATGACGACCATTACGTCAT
GCAGGAACATCGCAAGGTGCCCTTCGCCTGGTGTGCCCCCGAGAGCCTGAAGACACGCACCTTCTCCCATGCCAGCGACACCTGGATGTTCGGGGTGACA
CTGTGGGAAATGTTCACCTACGGCCAGGAGCCCTGGATCGGCCTCAACGGCAGTCAGATCCTGCATAAGATCGACAAGGAGGGGGAGCGGCTGCCCCGGC
CCGAGGACTGTCCCCAGGACATCTACAACGTCATGGTCCAGTGCTGGGCTCACAAGCCAGAGGACAGACCCACGTTTGTGGCCCTGCGGGACTTCCTGCT
GGAGGCCCAGCCCACAGACATGCGGGCCCTTCAGGACTTTGAGGAACCGGACAAGCTGCACATCCAGATGAATGATGTCATCACCGTCATCGAGGGAAGG
GCCGAGAACTACTGGTGGCGTGGCCAGAACACACGGACGCTGTGTGTGGGGCCCTTCCCTCGCAACGTGGTGACCTCCGTGGCCGGCCTGTCGGCCCAGG
ACATCAGCCAGCCCCTGCAGAACAGCTTCATCCACACAGGGCATGGCGACAGTGACCCCCGCCACTGCTGGGGCTTCCCGGACAGGATTGACGAACTGTA
TCTGGGAAACCCCATGGACCCCCCCGACCTCCTGAGCGTGGAACTGAGCACCTCCCGGCCCCCCCAGCATCTAGGAGGGGTGAAAAAACCAACCTATGAC
CCTGTGAGCGAGGACCAAGACCCCTTGTCCAGCGACTTCAAGAGGCTGGGCCTGCGGAAGCCAGGCCTGCCCCGAGGGCTGTGGCTGGCGAAGCCCTCGG
CGCGGGTGCCGGGCACCAAGGCCAGCCGAGGCAGCGGGGCTGAGGTCACGCTCATCGACTTCGGTGAGGAGCCCGTGGTCCCGGCCCTACGGCCCTGCGC
GCCCTCCCTGGCGCAGCTGGCCATGGACGCCTGCTCCCTGCTGGACGAGACCCCGCCTCAGAGCCCCACGCGGGCACTGCCCCGGCCCCTGCACCCCACG
CCTGTGGTGGACTGGGACGCACGCCCGCTGCCCCCCCCGCCCGCCTATGACGACGTGGCCCAGGATGAGGATGACTTTGAGATCTGCTCCATCAACAGCA
CCCTCGTGGGCGCGGGGGTCCCTGCCGGGCCCAGCCAGGGCCAGACCAACTACGCCTTTGTGCCTGAGCAGGCGCGGCCGCCCCCTCCCCTGGAGGACAA
CCTGTTCCTCCCGCCCCAGGGTGGGGGCAAGCCGCCCAGCTCCGCACAGACCGCAGAGATCTTCCAGGCGCTACAGCAGGAGTGCATGAGGCAACTGCAG
GCTCCGGCCGGCTCCCCGGCCCCCTCTCCCAGCCCGGGGGGTGACGACAAGCCCCAGGTGCCTCCTCGGGTACCCATCCCCCCTCGGCCCACGCGCCCAC
ACGTCCAGCTGTCTCCAGCCCCCCCGGGCGAGGAGGAGACCAGCCAGTGGCCTGGACCTGCTTCCCCTCCCCGGGTGCCTCCGCGGGAGCCCCTGTCCCC
TCAAGGCTCGAGGACACCCAGCCCCCTGGTACCACCTGGCAGCTCCCCGCTGCCACCCCGGCTCTCAAGCTCACCTGGGAAGACCATGCCCACCACCCAG
AGCTTTGCCTCAGACCCCAAGTACGCCACCCCCCAGGTGATCCAGGCCCCTGGCCCGCGGGCTGGTCCCTGCATCCTGCCCATCGTCCGGGATGGCAAGA
AGGTCAGCAGCACCCACTATTACTTGCTGCCCGAGCGACCATCCTACCTGGAGCGCTACCAGCGCTTCCTGCGTGAGGCCCAGAGCCCCGAGGAGCCTAC
CCCCCTGCCTGTGCCTCTGCTGCTGCCCCCACCCAGCACCCCAGCCCCCGCCGCCCCCACGGCCACCGTGCGGCCGATGCCCCAGGCTGCCTTGGACCCC
AAGGCCAACTTCTCCACCAACAACAGCAACCCAGGGGCCCGGCCACCACCCCCGAGGGCCACTGCTCGGCTGCCACAGAGGGGCTGCCCTGGCGATGGGC
CAGAGGCGGGCCGGCCAGCAGACAAGATCCAGATGGCCATGGTGCATGGGGTGACCACAGAGGAGTGCCAGGCGGCCCTGCAGTGCCACGGCTGGAGCGT
GCAGAGGGCTGCCCAGTATCTGAAGGTGGAGCAGCTCTTCGGGCTGGGTCTGCGGCCCAGAGGGGAGTGCCACAAAGTGCTGGAGATGTTCGACTGGAAC
CTGGAGCAGGCCGGCTGCCACCTTCTGGGCTCCTGGGGCCCTGCCCACCACAAGCGCTGAGATGCGTCTGGAGAGCCAGAGGGCCTGCCTGAAGGAATCA
CCTGAGCCTGTCCGTCCACCAGGAGTGGGGAGATGCCCCCATCCAGTCCTGGAGGACCCGCTGCTCCTGCTGCTCCCGGGGATGGAGCAAGGCCAAGGCT
GCGGGAGGCTGGGAGCCCTGCCCTGCCCATCCCTCCTGCACCAGCGCTGTCCCTGCACACTTTGGTTCAGTCCCGGTGCCCCTGCCAAGATGTGGAAGGG
GCCGGGTGAAGACAGGCTTGAGGGCTGCCCCAGCAGGCTCTGGGTATGACCTGCCTCTGGCCCTGGTCCTGGGCGGGGCCTGTGGGTGGAGTAGTACCCC
CAGGCCCTGCCCTGGGTGACAGACTGGGAGGAAACCAGGCTGGACCTGGGCAGGCGGGATGTGTTGGCCACAGGGAGAGGCGGACCGGCACCCGGTGGGA
CCTCCTAGGACTGGGCCTTCTTCCAGGGGGCCCCTGGCAGCAGCTGGGGTGTCGGGCAGAATGTGACTTGTGGCCTTACCATGGACTTGAATGGGACTTG
GCTGGCCTCAGGATCTTGTGCCTGGAAATAGCCTGAGGTGGCTCAGGAAGCGGAGAAAGGGTGCCAGACCATTCTCTGGCGGGGACCAGGGCCCAAGGCC
CCAGGGCTGGAAGGAGACCAAGGGGCAGCCGCCCTGGAGGGACATCAGTGCTTCCTCTTCCACCCAATTCCCCCACGCGGTTCCATGTTTTCCCACCAGC
CTGTTGGCGAAGTTGCTGCTCCGGCATTCAGTACCTGCTTCTTCCAGAGAAATAAAGTTAGTTTCTATTTTATGTT
  • refseq_TNK2.F1 (forward primer)
  • refseq_TNK2.R1 (reverse primer)
  • Single exon skipping

Frame 0

GPESSGELGERQWESVGREAVVLGAAPAPRSRP
ELVGRARPVGPRGPAGEGRLGVQEANWLPGVQEG
QVVRAVQETYLNGKEPQEGEGPGEGLRTRRRRR
RRRW*LAGPGPTVPPGLKGTQDVGAWGVGRRRV
RGTAELWEPLPEGRPRPAGTSSAVSAWASLKLCL
RGGSGRRQRLGGGRMQPEEGTGWLLELLSEVQL
QQYFLRLRDDLNVTRLSHFEYVKNEDLEKIGMG
RPGQRRLWEAVKRRKALCKRKSWMSKVFSGKRLE
AEFPPHHSQSTFRKTSPAPGGPAGEGPLQSLTC
LIGEKDLRLLEKLGDGSFGVVRRGEWDAPSGKT
VSVAVKCLKPDVLSQPEAMDDFIREVNAMHSLDH
RNLIRLYGVVLTPPMKMVTELAPLGSLLDRLRK
HQGHFLLGTLSRYAVQVAEGMGYLESKRFIHRD
LAARNLLLATRDLVKIGDFGLMRALPQNDDHYVM
QEHRKVPFAWCAPESLKTRTFSHASDTWMFGVT
LWEMFTYGQEPWIGLNGSQILHKIDKEGERLPR
PEDCPQDIYNVMVQCWAHKPEDRPTFVALRDFLL
EAQPTDMRALQDFEEPDKLHIQMNDVITVIEGR
AENYWWRGQNTRTLCVGPFPRNVVTSVAGLSAQ
DISQPLQNSFIHTGHGDSDPRHCWGFPDRIDELY
LGNPMDPPDLLSVELSTSRPPQHLGGVKKPTYD
PVSEDQDPLSSDFKRLGLRKPGLPRGLWLAKPS
ARVPGTKASRGSGAEVTLIDFGEEPVVPALRPCA
PSLAQLAMDACSLLDETPPQSPTRALPRPLHPT
PVVDWDARPLPPPPAYDDVAQDEDDFEICSINS
TLVGAGVPAGPSQGQTNYAFVPEQARPPPPLEDN
LFLPPQGGGKPPSSAQTAEIFQALQQECMRQLQ
APAGSPAPSPSPGGDDKPQVPPRVPIPPRPTRP
HVQLSPAPPGEEETSQWPGPASPPRVPPREPLSP
QGSRTPSPLVPPGSSPLPPRLSSSPGKTMPTTQ
SFASDPKYATPQVIQAPGPRAGPCILPIVRDGK
KVSSTHYYLLPERPSYLERYQRFLREAQSPEEPT
PLPVPLLLPPPSTPAPAAPTATVRPMPQAALDP
KANFSTNNSNPGARPPPPRATARLPQRGCPGDG
PEAGRPADKIQMAMVHGVTTEECQAALQCHGWSV
QRAAQYLKVEQLFGLGLRPRGECHKVLEMFDWN
LEQAGCHLLGSWGPAHHKR*DASGEPEGLPEGI
T*ACPSTRSGEMPPSSPGGPAAPAAPGDGARPRL
REAGSPALPIPPAPALSLHTLVQSRCPCQDVEG
AG*RQA*GLPQQALGMTCLWPWSWAGPVGGVVP
PGPALGDRLGGNQAGPGQAGCVGHRERRTGTRWD
LLGLGLLPGGPWQQLGCRAECDLWPYHGLEWDL
AGLRILCLEIA*GGSGSGERVPDHSLAGTRAQG
PRAGRRPRGSRPGGTSVLPLPPNSPTRFHVFPPA
CWRSCCSGIQYLLLPEK*S*FLFYV

Frame 1

GRKVPANWGSGSGRASGGRRWSWERPRRPEAAP
SWWGGPGRWGPGGLRGRGAWGCKRRIGCPGCRR
DRWCGLSRKPT*TGRSPRRERDPARGSGPGGAGG
GGGGDWQARAPRYLRG*RGRRM*GHGESGAEEC
GEPLSSGSHCLRAGRGRREPLLPSQPGRR*SCV
CGEAAEGGRGWEAAECSQRRAQAGCWSCCPRCSC
NSTSCGSEMTSTSPACPTLSTSRMRTWRRSAWV
GLASGGCGRL*RGGRPCANASRG*VRCSVESDW
RLSSHLITLRAPSGRPRPPLGAQQGRGPCRASPA
SLGRRTCASWRSWVMVPLAWCAGASGTRPQGRR
*VWL*SA*SPMS*ASQKPWTTSSGRSMPCTRST
TETSSASTGWCSRRP*RW*QSWHLWDRCWTGYVS
TRATSSWGL*AATLCRWLRAWATWSPSALFTVT
WLPAICCWLPATWSRSGTLG*CEHYLRMTTITS
CRNIARCPSPGVPPRA*RHAPSPMPATPGCSG*H
CGKCSPTARSPGSASTAVRSCIRSTRRGSGCPG
PRTVPRTSTTSWSSAGLTSQRTDPRLWPCGTSC
WRPSPQTCGPFRTLRNRTSCTSR*MMSSPSSREG
PRTTGGVARTHGRCVWGPSLATW*PPWPACRPR
TSASPCRTASSTQGMATVTPATAGASRTGLTNC
IWETPWTPPTS*AWN*APPGPPSI*EG*KNQPMT
L*ARTKTPCPATSRGWACGSQACPEGCGWRSPR
RGCRAPRPAEAAGLRSRSSTSVRSPWSRPYGPA
RPPWRSWPWTPAPCWTRPRLRAPRGHCPGPCTPR
LWWTGTHARCPPRPPMTTWPRMRMTLRSAPSTA
PSWARGSLPGPARARPTTPLCLSRRGRPLPWRT
TCSSRPRVGASRPAPHRPQRSSRRYSRSA*GNCR
LRPAPRPPLPARGVTTSPRCLLGYPSPLGPRAH
TSSCLQPPRARRRPASGLDLLPLPGCLRGSPCP
LKARGHPAPWYHLAAPRCHPGSQAHLGRPCPPPR
ALPQTPSTPPPR*SRPLARGLVPASCPSSGMAR
RSAAPTITCCPSDHPTWSATSASCVRPRAPRSL
PPCLCLCCCPHPAPQPPPPPRPPCGRCPRLPWTP
RPTSPPTTATQGPGHHPRGPLLGCHRGAALAMG
QRRAGQQTRSRWPWCMG*PQRSARRPCSATAGA
CRGLPSI*RWSSSSGWVCGPEGSATKCWRCSTGT
WSRPAATFWAPGALPTTSAEMRLESQRACLKES
PEPVRPPGVGRCPHPVLEDPLLLLLPGMEQGQG
CGRLGALPCPSLLHQRCPCTLWFSPGAPAKMWKG
PGEDRLEGCPSRLWV*PASGPGPGRGLWVE*YP
QALPWVTDWEETRLDLGRRDVLATGRGGPAPGG
TS*DWAFFQGAPGSSWGVGQNVTCGLTMDLNGTW
LASGSCAWK*PEVAQEAEKGCQTILWRGPGPKA
PGLEGDQGAAALEGHQCFLFHPIPPRGSMFSHQ
PVGEVAAPAFSTCFFQRNKVSFYFM

Frame 2

AGKFRRTGGAAVGERRAGGGGPGSGPGAPKPPR
AGGEGPAGGAPGACGGGAPGGARGELAARGAGG
TGGAGCPGNLPEREGAPGGRGTRRGAQDPEAPA
EEEVVTGRPGPHGTSGAEGDAGCRGMGSRAQKSA
GNR*ALGATA*GQAAAGGNLFCRLSLGVAEAVS
AGRQRKAAEAGRRQNAARGGHRLAAGAAVRGAA
ATVLPAAPR*PQRHPPVPL*VRQE*GPGEDRHGS
AWPAAAVGGCEEEEGLVQTQVVDE*GVQWKATG
G*VPTSSLSEHLPEDLARPWGPSRGGAPAEPHL
PHWGEGPAPPGEAG*WFLWRGAQGRVGRALREDG
ECGCEVPEARCPEPARSHGRLHPGGQCHALARP
PKPHPPLRGGAHAAHEDGDRAGTSGIVVGPAT*
APGPLPPGDSEPLRCAGG*GHGLPGVQALYSP*P
GCPQSAVGYPRPGQDRGLWADASTTSE*RPLRH
AGTSQGALRLVCPREPEDTHLLPCQRHLDVRGD
TVGNVHLRPGALDRPQRQSDPA*DRQGGGAAAPA
RGLSPGHLQRHGPVLGSQARGQTHVCGPAGLPA
GGPAHRHAGPSGL*GTGQAAHPDE*CHHRHRGK
GRELLVAWPEHTDAVCGALPSQRGDLRGRPVGPG
HQPAPAEQLHPHRAWRQ*PPPLLGLPGQD*RTV
SGKPHGPPRPPERGTEHLPAPPASRRGEKTNL*
PCERGPRPLVQRLQEAGPAEARPAPRAVAGEALG
AGAGHQGQPRQRG*GHAHRLR*GARGPGPTALR
ALPGAAGHGRLLPAGRDPASEPHAGTAPAPAPH
ACGGLGRTPAAPPARL*RRGPG*G*L*DLLHQQH
PRGRGGPCRAQPGPDQLRLCA*AGAAAPSPGGQ
PVPPAPGWGQAAQLRTDRRDLPGATAGVHEATA
GSGRLPGPLSQPGG*RQAPGASSGTHPPSAHAPT
RPAVSSPPGRGGDQPVAWTCFPSPGASAGAPVP
SRLEDTQPPGTTWQLPAATPALKLTWEDHAHHP
ELCLRPQVRHPPGDPGPWPAGWSLHPAHRPGWQE
GQQHPLLLAARATILPGALPALPA*GPEPRGAY
PPACASAAAPTQHPSPRRPHGHRAADAPGCLGP
QGQLLHQQQQPRGPATTPEGHCSAATEGLPWRWA
RGGPASRQDPDGHGAWGDHRGVPGGPAVPRLER
AEGCPVSEGGAALRAGSAAQRGVPQSAGDVRLE
PGAGRLPPSGLLGPCPPQALRCVWRARGPA*RNH
LSLSVHQEWGDAPIQSWRTRCSCCSRGWSKAKA
AGGWEPCPAHPSCTSAVPAHFGSVPVPLPRCGR
GRVKTGLRAAPAGSGYDLPLALVLGGACGWSSTP
RPCPG*QTGRKPGWTWAGGMCWPQGEADRHPVG
PPRTGPSSRGPLAAAGVSGRM*LVALPWT*MGL
GWPQDLVPGNSLRWLRKRRKGARPFSGGDQGPRP
QGWKETKGQPPWRDISASSSTQFPHAVPCFPTS
LLAKLLLRHSVPASSREIKLVSILC

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