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Sequence NM_025141
CCGGCCCGGAAGTGCGCAGGCGCTGGCAAGATGGCGGGAGGGGTGCTCCCGCTGAGGGGCCTCCGCGCCTTGTGTCGCGTGCTGCTCTTCCTCTCGCAGTTCTGCATTCTGTCGGGCGGTGAAAGTACTGAAATCCCACCTTATGTGATGAAGTGTCCGAGCAATGGTTTGTGTAGCAGGCTTCCTGCAGACTGTATAGA
CTGCACAACAAATTTCTCCTGTACCTATGGGAAGCCTGTCACTTTTGACTGTGCAGTGAAACCATCTGTTACCTGTGTTGATCAAGACTTCAAATCCCAA
AAGAACTTCATCATTAACATGACTTGCAGATTTTGCTGGCAGCTTCCTGAAACAGATTACGAGTGTACCAACTCCACCAGCTGCATGACGGTGTCCTGTC
CTCGGCAGCGCTACCCTGCCAACTGCACGGTGCGGGACCACGTCCACTGCTTGGGTAACCGTACTTTTCCCAAAATGCTATATTGCAATTGGACTGGAGG
CTATAAGTGGTCTACGGCTCTGGCTCTAAGCATCACCCTCGGTGGGTTTGGAGCAGACCGTTTCTACCTGGGCCAGTGGCGGGAAGGCCTCGGCAAGCTC
TTCAGCTTCGGTGGCCTGGGAATATGGACGCTGATAGACGTCCTGCTCATTGGAGTTGGCTATGTTGGACCAGCAGATGGCTCTTTGTACATTTAGCTGT
GGTGTGTGCTTCAGAAAGGAGCAGGGCTTAGAAAAAGCCCTTTTGTCCGTAGGAGTTGATGTGGTGTGAGTGATATATTTCTATGTTTTTAATGTACAGC
ATCTGTACTTTGTTTGCCTTGATAAAGGTAAGATAAATGAAACGCTGAACTATGCTAATCTGGAATTTGTTTTTATTTGCCTGAAATATATTTTTTTCTG
TGAAAAAATTAAAACGTACTTAAGCCAGGAGAATGAATTATACAGTGATTGAAAATCCATTTAATTCCTATGACTTTTGTTTTGTATTGCCCAAGTCAAA
CTACATCACTTGTATCTCCAGCCCAAATGTAGTCTGCCTTGAAAAGTCTTTCAGCTGTGACTGCAGGAAGTGGGAGTGTTTTTATTGTTAGCTAATTGCT
GTGACTGCAGGAAGTGGGAGTGTTTCTGTTGTTGGCTAATTGAAGTTATTAGGCTCAGCTTCAGTCATGTGTAAGTTTTGCAGTGTAATACATATGTAGT
CTGGTCTGTATATATGAAAATTTGAATTAAACTGCAGAATGTTTATGTCTAGTTATGGTTTAAATTTTCTTAGTAGTATATAAAAGGTAAGAGTACTGAA
AAATTAATAAAATTGCAAGTTAAGAAATA
- refseq_TM2D3.F1 (forward primer)
- refseq_TM2D3.R1 (reverse primer)
- Single exon skipping
Frame 0
PARKCAGAGKMAGGVLPLRGLRALCRVLLFLSQFCILSGGESTEIPPYVMKCPSNGLCSRLPADCID
CTTNFSCTYGKPVTFDCAVKPSVTCVDQDFKSQ
KNFIINMTCRFCWQLPETDYECTNSTSCMTVSC
PRQRYPANCTVRDHVHCLGNRTFPKMLYCNWTGG
YKWSTALALSITLGGFGADRFYLGQWREGLGKL
FSFGGLGIWTLIDVLLIGVGYVGPADGSLYI*L
WCVLQKGAGLRKSPFVRRS*CGVSDIFLCF*CTA
SVLCLP**R*DK*NAELC*SGICFYLPEIYFFL
*KN*NVLKPGE*IIQ*LKIHLIPMTFVLYCPSQ
TTSLVSPAQM*SALKSLSAVTAGSGSVFIVS*LL
*LQEVGVFLLLAN*SY*AQLQSCVSFAV*YICS
LVCIYENLN*TAECLCLVMV*IFLVVYKR*EY*
KINKIAS*EI
Frame 1
RPGSAQALARWREGCSR*GASAPCVACCSSSRSSAFCRAVKVLKSHLM**SVRAMVCVAGFLQTV*
TAQQISPVPMGSLSLLTVQ*NHLLPVLIKTSNPK
RTSSLT*LADFAGSFLKQITSVPTPPAA*RCPV
LGSATLPTARCGTTSTAWVTVLFPKCYIAIGLE
AISGLRLWL*ASPSVGLEQTVSTWASGGKASASS
SASVAWEYGR**TSCSLELAMLDQQMALCTFSC
GVCFRKEQGLEKALLSVGVDVV*VIYFYVFNVQ
HLYFVCLDKGKINETLNYANLEFVFICLKYIFFC
EKIKTYLSQENELYSD*KSI*FL*LLFCIAQVK
LHHLYLQPKCSLP*KVFQL*LQEVGVFLLLANC
CDCRKWECFCCWLIEVIRLSFSHV*VLQCNTYVV
WSVYMKI*IKLQNVYV*LWFKFS**YIKGKSTE
KLIKLQVKK
Frame 2
GPEVRRRWQDGGRGAPAEGPPRLVSRAALPLAVLHSVGR*KY*NPTLCDEVSEQWFV*QASCRLYR
LHNKFLLYLWEACHF*LCSETICYLC*SRLQIP
KELHH*HDLQILLAAS*NRLRVYQLHQLHDGVLS
SAALPCQLHGAGPRPLLG*PYFSQNAILQLDWR
L*VVYGSGSKHHPRWVWSRPFLPGPVAGRPRQA
LQLRWPGNMDADRRPAHWSWLCWTSRWLFVHLAV
VCASERSRA*KKPFCP*ELMWCE*YISMFLMYS
ICTLFALIKVR*MKR*TMLIWNLFLFA*NIFFS
VKKLKRT*ARRMNYTVIENPFNSYDFCFVLPKSN
YITCISSPNVVCLEKSFSCDCRKWECFYC*LIA
VTAGSGSVSVVG*LKLLGSASVMCKFCSVIHM*
SGLYI*KFELNCRMFMSSYGLNFLSSI*KVRVLK
N**NCKLRN
Sequence NM_078474
CCGGCCCGGAAGTGCGCAGGCGCTGGCAAGATGGCGGGAGGGGTGCTCCCGCTGAGGGGCCTCCGCGCCTTGTGTCGCGTGCTGCTCTTCCTCTCGCAGTTCTGCATTCTGTCGGGCGGTGAGCAATCGCAGGCGCTGGCTCAGTCAATAAAGGATCCGGGCCCAACACGCACATTCACAGTAGTTCCCAGGGCAGCAGA
AAGTACTGAAATCCCACCTTATGTGATGAAGTGTCCGAGCAATGGTTTGTGTAGCAGGCTTCCTGCAGACTGTATAGACTGCACAACAAATTTCTCCTGT
ACCTATGGGAAGCCTGTCACTTTTGACTGTGCAGTGAAACCATCTGTTACCTGTGTTGATCAAGACTTCAAATCCCAAAAGAACTTCATCATTAACATGA
CTTGCAGATTTTGCTGGCAGCTTCCTGAAACAGATTACGAGTGTACCAACTCCACCAGCTGCATGACGGTGTCCTGTCCTCGGCAGCGCTACCCTGCCAA
CTGCACGGTGCGGGACCACGTCCACTGCTTGGGTAACCGTACTTTTCCCAAAATGCTATATTGCAATTGGACTGGAGGCTATAAGTGGTCTACGGCTCTG
GCTCTAAGCATCACCCTCGGTGGGTTTGGAGCAGACCGTTTCTACCTGGGCCAGTGGCGGGAAGGCCTCGGCAAGCTCTTCAGCTTCGGTGGCCTGGGAA
TATGGACGCTGATAGACGTCCTGCTCATTGGAGTTGGCTATGTTGGACCAGCAGATGGCTCTTTGTACATTTAGCTGTGGTGTGTGCTTCAGAAAGGAGC
AGGGCTTAGAAAAAGCCCTTTTGTCCGTAGGAGTTGATGTGGTGTGAGTGATATATTTCTATGTTTTTAATGTACAGCATCTGTACTTTGTTTGCCTTGA
TAAAGGTAAGATAAATGAAACGCTGAACTATGCTAATCTGGAATTTGTTTTTATTTGCCTGAAATATATTTTTTTCTGTGAAAAAATTAAAACGTACTTA
AGCCAGGAGAATGAATTATACAGTGATTGAAAATCCATTTAATTCCTATGACTTTTGTTTTGTATTGCCCAAGTCAAACTACATCACTTGTATCTCCAGC
CCAAATGTAGTCTGCCTTGAAAAGTCTTTCAGCTGTGACTGCAGGAAGTGGGAGTGTTTTTATTGTTAGCTAATTGCTGTGACTGCAGGAAGTGGGAGTG
TTTCTGTTGTTGGCTAATTGAAGTTATTAGGCTCAGCTTCAGTCATGTGTAAGTTTTGCAGTGTAATACATATGTAGTCTGGTCTGTATATATGAAAATT
TGAATTAAACTGCAGAATGTTTATGTCTAGTTATGGTTTAAATTTTCTTAGTAGTATATAAAAGGTAAGAGTACTGAAAAATTAATAAAATTGCAAGTTA
AGAAATA
- refseq_TM2D3.F1 (forward primer)
- refseq_TM2D3.R1 (reverse primer)
- Single exon skipping
Frame 0
PARKCAGAGKMAGGVLPLRGLRALCRVLLFLSQFCILSGGEQSQALAQSIKDPGPTRTFTVVPRAAE
STEIPPYVMKCPSNGLCSRLPADCIDCTTNFSC
TYGKPVTFDCAVKPSVTCVDQDFKSQKNFIINM
TCRFCWQLPETDYECTNSTSCMTVSCPRQRYPAN
CTVRDHVHCLGNRTFPKMLYCNWTGGYKWSTAL
ALSITLGGFGADRFYLGQWREGLGKLFSFGGLG
IWTLIDVLLIGVGYVGPADGSLYI*LWCVLQKGA
GLRKSPFVRRS*CGVSDIFLCF*CTASVLCLP*
*R*DK*NAELC*SGICFYLPEIYFFL*KN*NVL
KPGE*IIQ*LKIHLIPMTFVLYCPSQTTSLVSPA
QM*SALKSLSAVTAGSGSVFIVS*LL*LQEVGV
FLLLAN*SY*AQLQSCVSFAV*YICSLVCIYEN
LN*TAECLCLVMV*IFLVVYKR*EY*KINKIAS*
EI
Frame 1
RPGSAQALARWREGCSR*GASAPCVACCSSSRSSAFCRAVSNRRRWLSQ*RIRAQHAHSQ*FPGQQ
KVLKSHLM**SVRAMVCVAGFLQTV*TAQQISPV
PMGSLSLLTVQ*NHLLPVLIKTSNPKRTSSLT*
LADFAGSFLKQITSVPTPPAA*RCPVLGSATLP
TARCGTTSTAWVTVLFPKCYIAIGLEAISGLRLW
L*ASPSVGLEQTVSTWASGGKASASSSASVAWE
YGR**TSCSLELAMLDQQMALCTFSCGVCFRKE
QGLEKALLSVGVDVV*VIYFYVFNVQHLYFVCLD
KGKINETLNYANLEFVFICLKYIFFCEKIKTYL
SQENELYSD*KSI*FL*LLFCIAQVKLHHLYLQ
PKCSLP*KVFQL*LQEVGVFLLLANCCDCRKWEC
FCCWLIEVIRLSFSHV*VLQCNTYVVWSVYMKI
*IKLQNVYV*LWFKFS**YIKGKSTEKLIKLQV
KK
Frame 2
GPEVRRRWQDGGRGAPAEGPPRLVSRAALPLAVLHSVGR*AIAGAGSVNKGSGPNTHIHSSSQGSR
KY*NPTLCDEVSEQWFV*QASCRLYRLHNKFLL
YLWEACHF*LCSETICYLC*SRLQIPKELHH*HD
LQILLAAS*NRLRVYQLHQLHDGVLSSAALPCQ
LHGAGPRPLLG*PYFSQNAILQLDWRL*VVYGS
GSKHHPRWVWSRPFLPGPVAGRPRQALQLRWPGN
MDADRRPAHWSWLCWTSRWLFVHLAVVCASERS
RA*KKPFCP*ELMWCE*YISMFLMYSICTLFAL
IKVR*MKR*TMLIWNLFLFA*NIFFSVKKLKRT*
ARRMNYTVIENPFNSYDFCFVLPKSNYITCISS
PNVVCLEKSFSCDCRKWECFYC*LIAVTAGSGS
VSVVG*LKLLGSASVMCKFCSVIHM*SGLYI*KF
ELNCRMFMSSYGLNFLSSI*KVRVLKN**NCKL
RN
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