Sequence NM_174967
ACTATGGCGGCGCCCATGCAGCCCAGCGCGTTGTGGGCTCCCGCCGGGGTCCCCCGCGGCTGTCGCCGCCGCCTACGCCGCTGCCTCCGCCTTCCTGCCC
CGCGTCGGGCCGGGCGCCACCTCCCCCCTGCCTCCCTCTCCGCTGTGGTCATTTAGGAAATCGTAAATCATGTGAAGATGGGACTCTTGGTATTTGTGCG
CAATCTGCTGCTAGCCCTCTGCCTCTTTCTGGTACTGGGATTTTTGTATTATTCTGCGTGGAAGCTACACTTACTCCAGTGGGAGGAGGACTCCAATTCA
GTGGTTCTTTCCTTTGACTCCGCTGGACAAACACTAGGCTCAGAGTATGATCGGTTGGGCTTCCTCCTGAATCTGGACTCTAAACTGCCTGCTGAATTAG
CCACCAAGTACGCAAACTTTTCAGAGGGAGCTTGCAAGCCTGGCTATGCTTCAGCCTTGATGACGGCCATCTTCCCCCGGTTCTCCAAGCCAGCACCCAT
GTTCCTGGATGACTCCTTTCGCAAGTGGGCTAGAATCCGGGAGTTCGTGCCGCCTTTTGGGATCAAAGGTCAAGTCCTGGACGCACAATATCCAGCGAGA
GAAAGAGTTTCTGCGGAAGCTGGTGAAAGCTCGCGTCATCACTGATCTAAGCAGTGGCATCTGAGTGGGCCCAGCACATGGCCATAGAGGCCCAGGCACC
ACCAGGAGCAGCAGCCAGCACCACCTACACAGGAGTCTTCAGACCCAGAGAAGGACGGTGCCAAGGGCCCCAGGGGCAGCAAGGCCTTGGTGGAGCAGCC
AGAGCTGTGCCTGCTCAGCAGCCAGTCTCAGAGACCAGCACTCAGCCTCATTCAGCATGGGTCCTTGATGCCAGAGGGCCAGCAGGCTCCTGGCTGTGCC
CAGCAGGCCCAGCATGCAGGTGGTGGGACACTGGGCAGCAAGGCTGCTGCCGGAATCACTTCTCCAATCAGTGTTTGGTGTATTATCATTTTGTGAATTT
GGGTAGGGGGGAGGGTAGGGATAATTTATTTTTAAATAAGGTTGGAGATGTCAAGTTGGGTTCACTTGCCATGCAGGAAGAGGCCCACTAGAGGGCCCAT
CAGGCAGTGTTACCTGTTAGCTCCCTGTGGGGCAGGAGTGCCAGGACCAGCCTGTACCTTGCTGTGGGGCTACAGGATGGTGGGCAGGATCTCAAGCCAG
CCCCCTCCAGCTCATGACACTGTTTGGCCTTTCTTGGGGAGAAGGCGGGGTATTCCCACTCACCAGCCCTAGCTGTCCCATGGGGAAACCCTGGAGCCAT
CCCTTCGGAGCCAACAAGACCGCCCCAGGGCTATAGCAGAAAGAACTTTAAAGCTCAGGAGGGTGACGCCCAGCTCCGCCTGCTGGGAAGAGCTCCCCTC
CACAGCTGCAGCTGATCCATAGGACTACCGCAGGCCCGGACTCACCAACTTGCCACATGTTCTAGGTTTCAGCAACAAGACTGCCAGGTGGTTGGGTTCT
GCCTTTAGCCTGGACCAAAGGGAAGTGAGGCCCAAGGAGCTTACCCAAGCTGTGGCAGCCGTCCCAGGCCACCCCCATGGAAGCAATAAAGCTCTTCCCT
GA
- refseq_ST3GAL3.F3 (forward primer)
- refseq_ST3GAL3.R3 (reverse primer)
- Alternative donor
Frame 0
TMAAPMQPSALWAPAGVPRGCRRRLRRCLRLPA
PRRAGRHLPPASLSAVVI*EIVNHVKMGLLVFVR
NLLLALCLFLVLGFLYYSAWKLHLLQWEEDSNS
VVLSFDSAGQTLGSEYDRLGFLLNLDSKLPAEL
ATKYANFSEGACKPGYASALMTAIFPRFSKPAPM
FLDDSFRKWARIREFVPPFGIKGQVLDAQYPAR
ERVSAEAGESSRHH*SKQWHLSGPSTWP*RPRH
HQEQQPAPPTQESSDPEKDGAKGPRGSKALVEQP
ELCLLSSQSQRPALSLIQHGSLMPEGQQAPGCA
QQAQHAGGGTLGSKAAAGITSPISVWCIIIL*I
WVGGRVGIIYF*IRLEMSSWVHLPCRKRPTRGPI
RQCYLLAPCGAGVPGPACTLLWGYRMVGRISSQ
PPPAHDTVWPFLGRRRGIPTHQP*LSHGETLEP
SLRSQQDRPRAIAERTLKLRRVTPSSACWEELPS
TAAADP*DYRRPGLTNLPHVLGFSNKTARWLGS
AFSLDQREVRPKELTQAVAAVPGHPHGSNKALP
*
Frame 1
LWRRPCSPARCGLPPGSPAAVAAAYAAASAFLP
RVGPGATSPLPPSPLWSFRKS*IM*RWDSWYLC
AICC*PSASFWYWDFCIILRGSYTYSSGRRTPIQ
WFFPLTPLDKH*AQSMIGWASS*IWTLNCLLN*
PPSTQTFQRELASLAMLQP**RPSSPGSPSQHP
CSWMTPFASGLESGSSCRLLGSKVKSWTHNIQRE
KEFLRKLVKARVITDLSSGI*VGPAHGHRGPGT
TRSSSQHHLHRSLQTQRRTVPRAPGAARPWWSS
QSCACSAASLRDQHSASFSMGP*CQRASRLLAVP
SRPSMQVVGHWAARLLPESLLQSVFGVLSFCEF
G*GGG*G*FIFK*GWRCQVGFTCHAGRGPLEGP
SGSVTC*LPVGQECQDQPVPCCGATGWWAGSQAS
PLQLMTLFGLSWGEGGVFPLTSPSCPMGKPWSH
PFGANKTAPGL*QKEL*SSGG*RPAPPAGKSSP
PQLQLIHRTTAGPDSPTCHMF*VSATRLPGGWVL
PLAWTKGK*GPRSLPKLWQPSQATPMEAIKLFP
Frame 2
YGGAHAAQRVVGSRRGPPRLSPPPTPLPPPSCP
ASGRAPPPPCLPLRCGHLGNRKSCEDGTLGICA
QSAASPLPLSGTGIFVLFCVEATLTPVGGGLQF
SGSFL*LRWTNTRLRV*SVGLPPESGL*TAC*IS
HQVRKLFRGSLQAWLCFSLDDGHLPPVLQASTH
VPG*LLSQVG*NPGVRAAFWDQRSSPGRTISSE
RKSFCGSW*KLASSLI*AVASEWAQHMAIEAQAP
PGAAASTTYTGVFRPREGRCQGPQGQQGLGGAA
RAVPAQQPVSETSTQPHSAWVLDARGPAGSWLC
PAGPACRWWDTGQQGCCRNHFSNQCLVYYHFVNL
GRGEGRDNLFLNKVGDVKLGSLAMQEEAH*RAH
QAVLPVSSLWGRSARTSLYLAVGLQDGGQDLKP
APSSS*HCLAFLGEKAGYSHSPALAVPWGNPGAI
PSEPTRPPQGYSRKNFKAQEGDAQLRLLGRAPL
HSCS*SIGLPQARTHQLATCSRFQQQDCQVVGF
CL*PGPKGSEAQGAYPSCGSRPRPPPWKQ*SSSL
Sequence NM_174968
ACTATGGCGGCGCCCATGCAGCCCAGCGCGTTGTGGGCTCCCGCCGGGGTCCCCCGCGGCTGTCGCCGCCGCCTACGCCGCTGCCTCCGCCTTCCTGCCC
CGCGTCGGGCCGGGCGCCACCTCCCCCCTGCCTCCCTCTCCGCTGTGGTCATTTAGGAAATCGTAAATCATGTGAAGATGGGACTCTTGGTATTTGTGCG
CAATCTGCTGCTAGCCCTCTGCCTCTTTCTGGTACTGGGATTTTTGTATTATTCTGCGTGGAAGCTACACTTACTCCAGTGGGAGGAGGACTCCAATTCA
GTGGTTCTTTCCTTTGACTCCGCTGGACAAACACTAGGCTCAGAGTATGATCGGTTGGGCTTCCTCCTGAATCTGGACTCTAAACTCTCACCGAGGACTC
TCTGCACGGTGGTTTTTGGCCTTGACTGCATATTGGAATCACCTGGAGAGCCTAAAAAATTACTGATGCCTGCATCCCACCCTCTAGAGATTTTGAAGTC
ACTGAGCGAGGACACAGCCTTTGCATTAGGATTTTTAAAGCTGCCCAGGCCTGCTGAATTAGCCACCAAGTACGCAAACTTTTCAGAGGGAGCTTGCAAG
CCTGGCTATGCTTCAGCCTTGATGACGGCCATCTTCCCCCGGTTCTCCAAGCCAGCACCCATGTTCCTGGATGACTCCTTTCGCAAGTGGGCTAGAATCC
GGGAGTTCGTGCCGCCTTTTGGGATCAAAGGTCAAGACAATCTGATCAAAGCCATCTTGTCAGTCACCAAAGAGTACCGCCTGACCCCTGCCTTGGACAG
CCTCCGCTGCCGCCGCTGCATCATCGTGGGCAATGGAGGCGTTCTTGCCAACAAGTCTCTGGGGTCACGAATTGACGACTATGACATTGTGGTGAGACTG
AATTCAGCACCAGTGAAAGGCTTTGAGAAGGACGTGGGCAGCAAAACGACACTGCGCATCACCTACCCCGAGGGCGCCATGCAGCGGCCTGAGCAGTACG
AGCGCGATTCTCTCTTTGTCCTCGCCGGCTTCAAGTGGCAGGACTTTAAGTGGTTGAAATACATCGTCTACAAGGAGAGAGTGAGTGCATCGGATGGCTT
CTGGAAATCTGTGGCCACTCGAGTGCCCAAGGAGCCCCCTGAGATTCGAATCCTCAACCCATATTTCATCCAGGAGGCCGCCTTCACCCTCATTGGCCTG
CCCTTCAACAATGGCCTCATGGGCCGGGGGAACATCCCTACCCTTGGCAGTGTGGCAGTGACCATGGCACTACACGGCTGTGACGAGGTGGCAGTCGCAG
GATTTGGCTATGACATGAGCACACCCAACGCACCCCTGCACTACTATGAGACCGTTCGCATGGCAGCCATCAAAGAGTCCTGGACGCACAATATCCAGCG
AGAGAAAGAGTTTCTGCGGAAGCTGGTGAAAGCTCGCGTCATCACTGATCTAAGCAGTGGCATCTGAGTGGGCCCAGCACATGGCCATAGAGGCCCAGGC
ACCACCAGGAGCAGCAGCCAGCACCACCTACACAGGAGTCTTCAGACCCAGAGAAGGACGGTGCCAAGGGCCCCAGGGGCAGCAAGGCCTTGGTGGAGCA
GCCAGAGCTGTGCCTGCTCAGCAGCCAGTCTCAGAGACCAGCACTCAGCCTCATTCAGCATGGGTCCTTGATGCCAGAGGGCCAGCAGGCTCCTGGCTGT
GCCCAGCAGGCCCAGCATGCAGGTGGTGGGACACTGGGCAGCAAGGCTGCTGCCGGAATCACTTCTCCAATCAGTGTTTGGTGTATTATCATTTTGTGAA
TTTGGGTAGGGGGGAGGGTAGGGATAATTTATTTTTAAATAAGGTTGGAGATGTCAAGTTGGGTTCACTTGCCATGCAGGAAGAGGCCCACTAGAGGGCC
CATCAGGCAGTGTTACCTGTTAGCTCCCTGTGGGGCAGGAGTGCCAGGACCAGCCTGTACCTTGCTGTGGGGCTACAGGATGGTGGGCAGGATCTCAAGC
CAGCCCCCTCCAGCTCATGACACTGTTTGGCCTTTCTTGGGGAGAAGGCGGGGTATTCCCACTCACCAGCCCTAGCTGTCCCATGGGGAAACCCTGGAGC
CATCCCTTCGGAGCCAACAAGACCGCCCCAGGGCTATAGCAGAAAGAACTTTAAAGCTCAGGAGGGTGACGCCCAGCTCCGCCTGCTGGGAAGAGCTCCC
CTCCACAGCTGCAGCTGATCCATAGGACTACCGCAGGCCCGGACTCACCAACTTGCCACATGTTCTAGGTTTCAGCAACAAGACTGCCAGGTGGTTGGGT
TCTGCCTTTAGCCTGGACCAAAGGGAAGTGAGGCCCAAGGAGCTTACCCAAGCTGTGGCAGCCGTCCCAGGCCACCCCCATGGAAGCAATAAAGCTCTTC
CCTGA
- refseq_ST3GAL3.F3 (forward primer)
- refseq_ST3GAL3.R3 (reverse primer)
- Alternative donor
Frame 0
TMAAPMQPSALWAPAGVPRGCRRRLRRCLRLPA
PRRAGRHLPPASLSAVVI*EIVNHVKMGLLVFVR
NLLLALCLFLVLGFLYYSAWKLHLLQWEEDSNS
VVLSFDSAGQTLGSEYDRLGFLLNLDSKLSPRT
LCTVVFGLDCILESPGEPKKLLMPASHPLEILKS
LSEDTAFALGFLKLPRPAELATKYANFSEGACK
PGYASALMTAIFPRFSKPAPMFLDDSFRKWARI
REFVPPFGIKGQDNLIKAILSVTKEYRLTPALDS
LRCRRCIIVGNGGVLANKSLGSRIDDYDIVVRL
NSAPVKGFEKDVGSKTTLRITYPEGAMQRPEQY
ERDSLFVLAGFKWQDFKWLKYIVYKERVSASDGF
WKSVATRVPKEPPEIRILNPYFIQEAAFTLIGL
PFNNGLMGRGNIPTLGSVAVTMALHGCDEVAVA
GFGYDMSTPNAPLHYYETVRMAAIKESWTHNIQR
EKEFLRKLVKARVITDLSSGI*VGPAHGHRGPG
TTRSSSQHHLHRSLQTQRRTVPRAPGAARPWWS
SQSCACSAASLRDQHSASFSMGP*CQRASRLLAV
PSRPSMQVVGHWAARLLPESLLQSVFGVLSFCE
FG*GGG*G*FIFK*GWRCQVGFTCHAGRGPLEG
PSGSVTC*LPVGQECQDQPVPCCGATGWWAGSQA
SPLQLMTLFGLSWGEGGVFPLTSPSCPMGKPWS
HPFGANKTAPGL*QKEL*SSGG*RPAPPAGKSS
PPQLQLIHRTTAGPDSPTCHMF*VSATRLPGGWV
LPLAWTKGK*GPRSLPKLWQPSQATPMEAIKLF
P
Frame 1
LWRRPCSPARCGLPPGSPAAVAAAYAAASAFLP
RVGPGATSPLPPSPLWSFRKS*IM*RWDSWYLC
AICC*PSASFWYWDFCIILRGSYTYSSGRRTPIQ
WFFPLTPLDKH*AQSMIGWASS*IWTLNSHRGL
SARWFLALTAYWNHLESLKNY*CLHPTL*RF*S
H*ARTQPLH*DF*SCPGLLN*PPSTQTFQRELAS
LAMLQP**RPSSPGSPSQHPCSWMTPFASGLES
GSSCRLLGSKVKTI*SKPSCQSPKSTA*PLPWT
ASAAAAASSWAMEAFLPTSLWGHELTTMTLW*D*
IQHQ*KALRRTWAAKRHCASPTPRAPCSGLSST
SAILSLSSPASSGRTLSG*NTSSTRRE*VHRMA
SGNLWPLECPRSPLRFESSTHISSRRPPSPSLAC
PSTMASWAGGTSLPLAVWQ*PWHYTAVTRWQSQ
DLAMT*AHPTHPCTTMRPFAWQPSKSPGRTISS
ERKSFCGSW*KLASSLI*AVASEWAQHMAIEAQA
PPGAAASTTYTGVFRPREGRCQGPQGQQGLGGA
ARAVPAQQPVSETSTQPHSAWVLDARGPAGSWL
CPAGPACRWWDTGQQGCCRNHFSNQCLVYYHFVN
LGRGEGRDNLFLNKVGDVKLGSLAMQEEAH*RA
HQAVLPVSSLWGRSARTSLYLAVGLQDGGQDLK
PAPSSS*HCLAFLGEKAGYSHSPALAVPWGNPGA
IPSEPTRPPQGYSRKNFKAQEGDAQLRLLGRAP
LHSCS*SIGLPQARTHQLATCSRFQQQDCQVVG
FCL*PGPKGSEAQGAYPSCGSRPRPPPWKQ*SSS
L
Frame 2
YGGAHAAQRVVGSRRGPPRLSPPPTPLPPPSCP
ASGRAPPPPCLPLRCGHLGNRKSCEDGTLGICA
QSAASPLPLSGTGIFVLFCVEATLTPVGGGLQF
SGSFL*LRWTNTRLRV*SVGLPPESGL*TLTEDS
LHGGFWP*LHIGITWRA*KITDACIPPSRDFEV
TERGHSLCIRIFKAAQAC*ISHQVRKLFRGSLQ
AWLCFSLDDGHLPPVLQASTHVPG*LLSQVG*NP
GVRAAFWDQRSRQSDQSHLVSHQRVPPDPCLGQ
PPLPPLHHRGQWRRSCQQVSGVTN*RL*HCGET
EFSTSERL*EGRGQQNDTAHHLPRGRHAAA*AVR
ARFSLCPRRLQVAGL*VVEIHRLQGESECIGWL
LEICGHSSAQGAP*DSNPQPIFHPGGRLHPHWP
ALQQWPHGPGEHPYPWQCGSDHGTTRL*RGGSRR
IWL*HEHTQRTPALL*DRSHGSHQRVLDAQYPA
RERVSAEAGESSRHH*SKQWHLSGPSTWP*RPR
HHQEQQPAPPTQESSDPEKDGAKGPRGSKALVEQ
PELCLLSSQSQRPALSLIQHGSLMPEGQQAPGC
AQQAQHAGGGTLGSKAAAGITSPISVWCIIIL*
IWVGGRVGIIYF*IRLEMSSWVHLPCRKRPTRGP
IRQCYLLAPCGAGVPGPACTLLWGYRMVGRISS
QPPPAHDTVWPFLGRRRGIPTHQP*LSHGETLE
PSLRSQQDRPRAIAERTLKLRRVTPSSACWEELP
STAAADP*DYRRPGLTNLPHVLGFSNKTARWLG
SAFSLDQREVRPKELTQAVAAVPGHPHGSNKAL
P*
Sequence NM_174966
ACTATGGCGGCGCCCATGCAGCCCAGCGCGTTGTGGGCTCCCGCCGGGGTCCCCCGCGGCTGTCGCCGCCGCCTACGCCGCTGCCTCCGCCTTCCTGCCC
CGCGTCGGGCCGGGCGCCACCTCCCCCCTGCCTCCCTCTCCGCTGTGGTCATTTAGGAAATCGTAAATCATGTGAAGATGGGACTCTTGGTATTTGTGCG
CAATCTGCTGCTAGCCCTCTGCCTCTTTCTGGTACTGGGATTTTTGTATTATTCTGCGTGGAAGCTACACTTACTCCAGTGGGAGGAGGACTCCAATTCA
GTGGTTCTTTCCTTTGACTCCGCTGGACAAACACTAGGCTCAGAGTATGATCGGTTGGGCTTCCTCCTGAATCTGGACTCTAAACTGCCTGCTGAATTAG
CCACCAAGTACGCAAACTTTTCAGAGGGAGCTTGCAAGCCTGGCTATGCTTCAGCCTTGATGACGGCCATCTTCCCCCGGTTCTCCAAGCCAGCACCCAT
GTTCCTGGATGACTCCTTTCGCAAGTGGGCTAGAATCCGGGAGTTCGTGCCGCCTTTTGGGATCAAAGGTCAAGACAATCTGATCAAAGCCATCTTGTCA
GTCACCAAAGAGTACCGCCTGACCCCTGCCTTGGACAGCCTCCGCTGCCGCCGCTGCATCATCGTGGGCAATGGAGGCGTTCTTGCCAACAAGTCTCTGG
GGTCACGAATTGACGACTATGACATTGTGGTGAGACTGAATTCAGCACCAGTGAAAGGCTTTGAGAAGGACGTGGGCAGCAAAACGACACTGCGCATCAC
CTACCCCGAGGGCGCCATGCAGCGGCCTGAGCAGTACGAGCGCGATTCTCTCTTTGTCCTCGCCGGCTTCAAGTGGCAGGACTTTAAGTGGTTGAAATAC
ATCGTCTACAAGGAGAGAGTGTCCTGGACGCACAATATCCAGCGAGAGAAAGAGTTTCTGCGGAAGCTGGTGAAAGCTCGCGTCATCACTGATCTAAGCA
GTGGCATCTGAGTGGGCCCAGCACATGGCCATAGAGGCCCAGGCACCACCAGGAGCAGCAGCCAGCACCACCTACACAGGAGTCTTCAGACCCAGAGAAG
GACGGTGCCAAGGGCCCCAGGGGCAGCAAGGCCTTGGTGGAGCAGCCAGAGCTGTGCCTGCTCAGCAGCCAGTCTCAGAGACCAGCACTCAGCCTCATTC
AGCATGGGTCCTTGATGCCAGAGGGCCAGCAGGCTCCTGGCTGTGCCCAGCAGGCCCAGCATGCAGGTGGTGGGACACTGGGCAGCAAGGCTGCTGCCGG
AATCACTTCTCCAATCAGTGTTTGGTGTATTATCATTTTGTGAATTTGGGTAGGGGGGAGGGTAGGGATAATTTATTTTTAAATAAGGTTGGAGATGTCA
AGTTGGGTTCACTTGCCATGCAGGAAGAGGCCCACTAGAGGGCCCATCAGGCAGTGTTACCTGTTAGCTCCCTGTGGGGCAGGAGTGCCAGGACCAGCCT
GTACCTTGCTGTGGGGCTACAGGATGGTGGGCAGGATCTCAAGCCAGCCCCCTCCAGCTCATGACACTGTTTGGCCTTTCTTGGGGAGAAGGCGGGGTAT
TCCCACTCACCAGCCCTAGCTGTCCCATGGGGAAACCCTGGAGCCATCCCTTCGGAGCCAACAAGACCGCCCCAGGGCTATAGCAGAAAGAACTTTAAAG
CTCAGGAGGGTGACGCCCAGCTCCGCCTGCTGGGAAGAGCTCCCCTCCACAGCTGCAGCTGATCCATAGGACTACCGCAGGCCCGGACTCACCAACTTGC
CACATGTTCTAGGTTTCAGCAACAAGACTGCCAGGTGGTTGGGTTCTGCCTTTAGCCTGGACCAAAGGGAAGTGAGGCCCAAGGAGCTTACCCAAGCTGT
GGCAGCCGTCCCAGGCCACCCCCATGGAAGCAATAAAGCTCTTCCCTGA
- refseq_ST3GAL3.F3 (forward primer)
- refseq_ST3GAL3.R3 (reverse primer)
- Alternative donor
Frame 0
TMAAPMQPSALWAPAGVPRGCRRRLRRCLRLPA
PRRAGRHLPPASLSAVVI*EIVNHVKMGLLVFVR
NLLLALCLFLVLGFLYYSAWKLHLLQWEEDSNS
VVLSFDSAGQTLGSEYDRLGFLLNLDSKLPAEL
ATKYANFSEGACKPGYASALMTAIFPRFSKPAPM
FLDDSFRKWARIREFVPPFGIKGQDNLIKAILS
VTKEYRLTPALDSLRCRRCIIVGNGGVLANKSL
GSRIDDYDIVVRLNSAPVKGFEKDVGSKTTLRIT
YPEGAMQRPEQYERDSLFVLAGFKWQDFKWLKY
IVYKERVSWTHNIQREKEFLRKLVKARVITDLS
SGI*VGPAHGHRGPGTTRSSSQHHLHRSLQTQRR
TVPRAPGAARPWWSSQSCACSAASLRDQHSASF
SMGP*CQRASRLLAVPSRPSMQVVGHWAARLLP
ESLLQSVFGVLSFCEFG*GGG*G*FIFK*GWRCQ
VGFTCHAGRGPLEGPSGSVTC*LPVGQECQDQP
VPCCGATGWWAGSQASPLQLMTLFGLSWGEGGV
FPLTSPSCPMGKPWSHPFGANKTAPGL*QKEL*S
SGG*RPAPPAGKSSPPQLQLIHRTTAGPDSPTC
HMF*VSATRLPGGWVLPLAWTKGK*GPRSLPKL
WQPSQATPMEAIKLFP
Frame 1
LWRRPCSPARCGLPPGSPAAVAAAYAAASAFLP
RVGPGATSPLPPSPLWSFRKS*IM*RWDSWYLC
AICC*PSASFWYWDFCIILRGSYTYSSGRRTPIQ
WFFPLTPLDKH*AQSMIGWASS*IWTLNCLLN*
PPSTQTFQRELASLAMLQP**RPSSPGSPSQHP
CSWMTPFASGLESGSSCRLLGSKVKTI*SKPSCQ
SPKSTA*PLPWTASAAAAASSWAMEAFLPTSLW
GHELTTMTLW*D*IQHQ*KALRRTWAAKRHCAS
PTPRAPCSGLSSTSAILSLSSPASSGRTLSG*NT
SSTRRECPGRTISSERKSFCGSW*KLASSLI*A
VASEWAQHMAIEAQAPPGAAASTTYTGVFRPRE
GRCQGPQGQQGLGGAARAVPAQQPVSETSTQPHS
AWVLDARGPAGSWLCPAGPACRWWDTGQQGCCR
NHFSNQCLVYYHFVNLGRGEGRDNLFLNKVGDV
KLGSLAMQEEAH*RAHQAVLPVSSLWGRSARTSL
YLAVGLQDGGQDLKPAPSSS*HCLAFLGEKAGY
SHSPALAVPWGNPGAIPSEPTRPPQGYSRKNFK
AQEGDAQLRLLGRAPLHSCS*SIGLPQARTHQLA
TCSRFQQQDCQVVGFCL*PGPKGSEAQGAYPSC
GSRPRPPPWKQ*SSSL
Frame 2
YGGAHAAQRVVGSRRGPPRLSPPPTPLPPPSCP
ASGRAPPPPCLPLRCGHLGNRKSCEDGTLGICA
QSAASPLPLSGTGIFVLFCVEATLTPVGGGLQF
SGSFL*LRWTNTRLRV*SVGLPPESGL*TAC*IS
HQVRKLFRGSLQAWLCFSLDDGHLPPVLQASTH
VPG*LLSQVG*NPGVRAAFWDQRSRQSDQSHLV
SHQRVPPDPCLGQPPLPPLHHRGQWRRSCQQVSG
VTN*RL*HCGETEFSTSERL*EGRGQQNDTAHH
LPRGRHAAA*AVRARFSLCPRRLQVAGL*VVEI
HRLQGESVLDAQYPARERVSAEAGESSRHH*SKQ
WHLSGPSTWP*RPRHHQEQQPAPPTQESSDPEK
DGAKGPRGSKALVEQPELCLLSSQSQRPALSLI
QHGSLMPEGQQAPGCAQQAQHAGGGTLGSKAAAG
ITSPISVWCIIIL*IWVGGRVGIIYF*IRLEMS
SWVHLPCRKRPTRGPIRQCYLLAPCGAGVPGPA
CTLLWGYRMVGRISSQPPPAHDTVWPFLGRRRGI
PTHQP*LSHGETLEPSLRSQQDRPRAIAERTLK
LRRVTPSSACWEELPSTAAADP*DYRRPGLTNL
PHVLGFSNKTARWLGSAFSLDQREVRPKELTQAV
AAVPGHPHGSNKALP*
Sequence NM_006279
ACTATGGCGGCGCCCATGCAGCCCAGCGCGTTGTGGGCTCCCGCCGGGGTCCCCCGCGGCTGTCGCCGCCGCCTACGCCGCTGCCTCCGCCTTCCTGCCC
CGCGTCGGGCCGGGCGCCACCTCCCCCCTGCCTCCCTCTCCGCTGTGGTCATTTAGGAAATCGTAAATCATGTGAAGATGGGACTCTTGGTATTTGTGCG
CAATCTGCTGCTAGCCCTCTGCCTCTTTCTGGTACTGGGATTTTTGTATTATTCTGCGTGGAAGCTACACTTACTCCAGTGGGAGGAGGACTCCAATTCA
GTGGTTCTTTCCTTTGACTCCGCTGGACAAACACTAGGCTCAGAGTATGATCGGTTGGGCTTCCTCCTGAATCTGGACTCTAAACTGCCTGCTGAATTAG
CCACCAAGTACGCAAACTTTTCAGAGGGAGCTTGCAAGCCTGGCTATGCTTCAGCCTTGATGACGGCCATCTTCCCCCGGTTCTCCAAGCCAGCACCCAT
GTTCCTGGATGACTCCTTTCGCAAGTGGGCTAGAATCCGGGAGTTCGTGCCGCCTTTTGGGATCAAAGGTCAAGACAATCTGATCAAAGCCATCTTGTCA
GTCACCAAAGAGTACCGCCTGACCCCTGCCTTGGACAGCCTCCGCTGCCGCCGCTGCATCATCGTGGGCAATGGAGGCGTTCTTGCCAACAAGTCTCTGG
GGTCACGAATTGACGACTATGACATTGTGGTGAGACTGAATTCAGCACCAGTGAAAGGCTTTGAGAAGGACGTGGGCAGCAAAACGACACTGCGCATCAC
CTACCCCGAGGGCGCCATGCAGCGGCCTGAGCAGTACGAGCGCGATTCTCTCTTTGTCCTCGCCGGCTTCAAGTGGCAGGACTTTAAGTGGTTGAAATAC
ATCGTCTACAAGGAGAGAGTGAGTGCATCGGATGGCTTCTGGAAATCTGTGGCCACTCGAGTGCCCAAGGAGCCCCCTGAGATTCGAATCCTCAACCCAT
ATTTCATCCAGGAGGCCGCCTTCACCCTCATTGGCCTGCCCTTCAACAATGGCCTCATGGGCCGGGGGAACATCCCTACCCTTGGCAGTGTGGCAGTGAC
CATGGCACTACACGGCTGTGACGAGGTGGCAGTCGCAGGATTTGGCTATGACATGAGCACACCCAACGCACCCCTGCACTACTATGAGACCGTTCGCATG
GCAGCCATCAAAGAGTCCTGGACGCACAATATCCAGCGAGAGAAAGAGTTTCTGCGGAAGCTGGTGAAAGCTCGCGTCATCACTGATCTAAGCAGTGGCA
TCTGAGTGGGCCCAGCACATGGCCATAGAGGCCCAGGCACCACCAGGAGCAGCAGCCAGCACCACCTACACAGGAGTCTTCAGACCCAGAGAAGGACGGT
GCCAAGGGCCCCAGGGGCAGCAAGGCCTTGGTGGAGCAGCCAGAGCTGTGCCTGCTCAGCAGCCAGTCTCAGAGACCAGCACTCAGCCTCATTCAGCATG
GGTCCTTGATGCCAGAGGGCCAGCAGGCTCCTGGCTGTGCCCAGCAGGCCCAGCATGCAGGTGGTGGGACACTGGGCAGCAAGGCTGCTGCCGGAATCAC
TTCTCCAATCAGTGTTTGGTGTATTATCATTTTGTGAATTTGGGTAGGGGGGAGGGTAGGGATAATTTATTTTTAAATAAGGTTGGAGATGTCAAGTTGG
GTTCACTTGCCATGCAGGAAGAGGCCCACTAGAGGGCCCATCAGGCAGTGTTACCTGTTAGCTCCCTGTGGGGCAGGAGTGCCAGGACCAGCCTGTACCT
TGCTGTGGGGCTACAGGATGGTGGGCAGGATCTCAAGCCAGCCCCCTCCAGCTCATGACACTGTTTGGCCTTTCTTGGGGAGAAGGCGGGGTATTCCCAC
TCACCAGCCCTAGCTGTCCCATGGGGAAACCCTGGAGCCATCCCTTCGGAGCCAACAAGACCGCCCCAGGGCTATAGCAGAAAGAACTTTAAAGCTCAGG
AGGGTGACGCCCAGCTCCGCCTGCTGGGAAGAGCTCCCCTCCACAGCTGCAGCTGATCCATAGGACTACCGCAGGCCCGGACTCACCAACTTGCCACATG
TTCTAGGTTTCAGCAACAAGACTGCCAGGTGGTTGGGTTCTGCCTTTAGCCTGGACCAAAGGGAAGTGAGGCCCAAGGAGCTTACCCAAGCTGTGGCAGC
CGTCCCAGGCCACCCCCATGGAAGCAATAAAGCTCTTCCCTGA
- refseq_ST3GAL3.F3 (forward primer)
- refseq_ST3GAL3.R3 (reverse primer)
- Alternative donor
Frame 0
TMAAPMQPSALWAPAGVPRGCRRRLRRCLRLPA
PRRAGRHLPPASLSAVVI*EIVNHVKMGLLVFVR
NLLLALCLFLVLGFLYYSAWKLHLLQWEEDSNS
VVLSFDSAGQTLGSEYDRLGFLLNLDSKLPAEL
ATKYANFSEGACKPGYASALMTAIFPRFSKPAPM
FLDDSFRKWARIREFVPPFGIKGQDNLIKAILS
VTKEYRLTPALDSLRCRRCIIVGNGGVLANKSL
GSRIDDYDIVVRLNSAPVKGFEKDVGSKTTLRIT
YPEGAMQRPEQYERDSLFVLAGFKWQDFKWLKY
IVYKERVSASDGFWKSVATRVPKEPPEIRILNP
YFIQEAAFTLIGLPFNNGLMGRGNIPTLGSVAVT
MALHGCDEVAVAGFGYDMSTPNAPLHYYETVRM
AAIKESWTHNIQREKEFLRKLVKARVITDLSSG
I*VGPAHGHRGPGTTRSSSQHHLHRSLQTQRRTV
PRAPGAARPWWSSQSCACSAASLRDQHSASFSM
GP*CQRASRLLAVPSRPSMQVVGHWAARLLPES
LLQSVFGVLSFCEFG*GGG*G*FIFK*GWRCQVG
FTCHAGRGPLEGPSGSVTC*LPVGQECQDQPVP
CCGATGWWAGSQASPLQLMTLFGLSWGEGGVFP
LTSPSCPMGKPWSHPFGANKTAPGL*QKEL*SSG
G*RPAPPAGKSSPPQLQLIHRTTAGPDSPTCHM
F*VSATRLPGGWVLPLAWTKGK*GPRSLPKLWQ
PSQATPMEAIKLFP
Frame 1
LWRRPCSPARCGLPPGSPAAVAAAYAAASAFLP
RVGPGATSPLPPSPLWSFRKS*IM*RWDSWYLC
AICC*PSASFWYWDFCIILRGSYTYSSGRRTPIQ
WFFPLTPLDKH*AQSMIGWASS*IWTLNCLLN*
PPSTQTFQRELASLAMLQP**RPSSPGSPSQHP
CSWMTPFASGLESGSSCRLLGSKVKTI*SKPSCQ
SPKSTA*PLPWTASAAAAASSWAMEAFLPTSLW
GHELTTMTLW*D*IQHQ*KALRRTWAAKRHCAS
PTPRAPCSGLSSTSAILSLSSPASSGRTLSG*NT
SSTRRE*VHRMASGNLWPLECPRSPLRFESSTH
ISSRRPPSPSLACPSTMASWAGGTSLPLAVWQ*
PWHYTAVTRWQSQDLAMT*AHPTHPCTTMRPFAW
QPSKSPGRTISSERKSFCGSW*KLASSLI*AVA
SEWAQHMAIEAQAPPGAAASTTYTGVFRPREGR
CQGPQGQQGLGGAARAVPAQQPVSETSTQPHSAW
VLDARGPAGSWLCPAGPACRWWDTGQQGCCRNH
FSNQCLVYYHFVNLGRGEGRDNLFLNKVGDVKL
GSLAMQEEAH*RAHQAVLPVSSLWGRSARTSLYL
AVGLQDGGQDLKPAPSSS*HCLAFLGEKAGYSH
SPALAVPWGNPGAIPSEPTRPPQGYSRKNFKAQ
EGDAQLRLLGRAPLHSCS*SIGLPQARTHQLATC
SRFQQQDCQVVGFCL*PGPKGSEAQGAYPSCGS
RPRPPPWKQ*SSSL
Frame 2
YGGAHAAQRVVGSRRGPPRLSPPPTPLPPPSCP
ASGRAPPPPCLPLRCGHLGNRKSCEDGTLGICA
QSAASPLPLSGTGIFVLFCVEATLTPVGGGLQF
SGSFL*LRWTNTRLRV*SVGLPPESGL*TAC*IS
HQVRKLFRGSLQAWLCFSLDDGHLPPVLQASTH
VPG*LLSQVG*NPGVRAAFWDQRSRQSDQSHLV
SHQRVPPDPCLGQPPLPPLHHRGQWRRSCQQVSG
VTN*RL*HCGETEFSTSERL*EGRGQQNDTAHH
LPRGRHAAA*AVRARFSLCPRRLQVAGL*VVEI
HRLQGESECIGWLLEICGHSSAQGAP*DSNPQPI
FHPGGRLHPHWPALQQWPHGPGEHPYPWQCGSD
HGTTRL*RGGSRRIWL*HEHTQRTPALL*DRSH
GSHQRVLDAQYPARERVSAEAGESSRHH*SKQWH
LSGPSTWP*RPRHHQEQQPAPPTQESSDPEKDG
AKGPRGSKALVEQPELCLLSSQSQRPALSLIQH
GSLMPEGQQAPGCAQQAQHAGGGTLGSKAAAGIT
SPISVWCIIIL*IWVGGRVGIIYF*IRLEMSSW
VHLPCRKRPTRGPIRQCYLLAPCGAGVPGPACT
LLWGYRMVGRISSQPPPAHDTVWPFLGRRRGIPT
HQP*LSHGETLEPSLRSQQDRPRAIAERTLKLR
RVTPSSACWEELPSTAAADP*DYRRPGLTNLPH
VLGFSNKTARWLGSAFSLDQREVRPKELTQAVAA
VPGHPHGSNKALP*
Sequence NM_174963
ACTATGGCGGCGCCCATGCAGCCCAGCGCGTTGTGGGCTCCCGCCGGGGTCCCCCGCGGCTGTCGCCGCCGCCTACGCCGCTGCCTCCGCCTTCCTGCCC
CGCGTCGGGCCGGGCGCCACCTCCCCCCTGCCTCCCTCTCCGCTGTGGTCATTTAGGAAATCGTAAATCATGTGAAGATGGGACTCTTGGTATTTGTGCG
CAATCTGCTGCTAGCCCTCTGCCTCTTTCTGGTACTGGGATTTTTGTATTATTCTGCGTGGAAGCTACACTTACTCCAGTGGGAGGAGGACTCCAGTAAG
TATAGTCACTCTAGCTCACCCCAGGAGAAGCCTGTTGCAGATTCAGTGGTTCTTTCCTTTGACTCCGCTGGACAAACACTAGGCTCAGAGTATGATCGGT
TGGGCTTCCTCCTGAATCTGGACTCTAAACTCTCACCGAGGACTCTCTGCACGGTGGTTTTTGGCCTTGACTGCATATTGGAATCACCTGGAGAGCCTAA
AAAATTACTGATGCCTGCATCCCACCCTCTAGAGATTTTGAAGTCACTGAGCGAGGACACAGCCTTTGCATTAGGATTTTTAAAGCTGCCCAGGCCTGCT
GAATTAGCCACCAAGTACGCAAACTTTTCAGAGGGAGCTTGCAAGCCTGGCTATGCTTCAGCCTTGATGACGGCCATCTTCCCCCGGTTCTCCAAGCCAG
CACCCATGTTCCTGGATGACTCCTTTCGCAAGTGGGCTAGAATCCGGGAGTTCGTGCCGCCTTTTGGGATCAAAGGTCAAGACAATCTGATCAAAGCCAT
CTTGTCAGTCACCAAAGAGTACCGCCTGACCCCTGCCTTGGACAGCCTCCGCTGCCGCCGCTGCATCATCGTGGGCAATGGAGGCGTTCTTGCCAACAAG
TCTCTGGGGTCACGAATTGACGACTATGACATTGTGGTGAGACTGAATTCAGCACCAGTGAAAGGCTTTGAGAAGGACGTGGGCAGCAAAACGACACTGC
GCATCACCTACCCCGAGGGCGCCATGCAGCGGCCTGAGCAGTACGAGCGCGATTCTCTCTTTGTCCTCGCCGGCTTCAAGTGGCAGGACTTTAAGTGGTT
GAAATACATCGTCTACAAGGAGAGAGTGAGTGCATCGGATGGCTTCTGGAAATCTGTGGCCACTCGAGTGCCCAAGGAGCCCCCTGAGATTCGAATCCTC
AACCCATATTTCATCCAGGAGGCCGCCTTCACCCTCATTGGCCTGCCCTTCAACAATGGCCTCATGGGCCGGGGGAACATCCCTACCCTTGGCAGTGTGG
CAGTGACCATGGCACTACACGGCTGTGACGAGGTGGCAGTCGCAGGATTTGGCTATGACATGAGCACACCCAACGCACCCCTGCACTACTATGAGACCGT
TCGCATGGCAGCCATCAAAGAGTCCTGGACGCACAATATCCAGCGAGAGAAAGAGTTTCTGCGGAAGCTGGTGAAAGCTCGCGTCATCACTGATCTAAGC
AGTGGCATCTGAGTGGGCCCAGCACATGGCCATAGAGGCCCAGGCACCACCAGGAGCAGCAGCCAGCACCACCTACACAGGAGTCTTCAGACCCAGAGAA
GGACGGTGCCAAGGGCCCCAGGGGCAGCAAGGCCTTGGTGGAGCAGCCAGAGCTGTGCCTGCTCAGCAGCCAGTCTCAGAGACCAGCACTCAGCCTCATT
CAGCATGGGTCCTTGATGCCAGAGGGCCAGCAGGCTCCTGGCTGTGCCCAGCAGGCCCAGCATGCAGGTGGTGGGACACTGGGCAGCAAGGCTGCTGCCG
GAATCACTTCTCCAATCAGTGTTTGGTGTATTATCATTTTGTGAATTTGGGTAGGGGGGAGGGTAGGGATAATTTATTTTTAAATAAGGTTGGAGATGTC
AAGTTGGGTTCACTTGCCATGCAGGAAGAGGCCCACTAGAGGGCCCATCAGGCAGTGTTACCTGTTAGCTCCCTGTGGGGCAGGAGTGCCAGGACCAGCC
TGTACCTTGCTGTGGGGCTACAGGATGGTGGGCAGGATCTCAAGCCAGCCCCCTCCAGCTCATGACACTGTTTGGCCTTTCTTGGGGAGAAGGCGGGGTA
TTCCCACTCACCAGCCCTAGCTGTCCCATGGGGAAACCCTGGAGCCATCCCTTCGGAGCCAACAAGACCGCCCCAGGGCTATAGCAGAAAGAACTTTAAA
GCTCAGGAGGGTGACGCCCAGCTCCGCCTGCTGGGAAGAGCTCCCCTCCACAGCTGCAGCTGATCCATAGGACTACCGCAGGCCCGGACTCACCAACTTG
CCACATGTTCTAGGTTTCAGCAACAAGACTGCCAGGTGGTTGGGTTCTGCCTTTAGCCTGGACCAAAGGGAAGTGAGGCCCAAGGAGCTTACCCAAGCTG
TGGCAGCCGTCCCAGGCCACCCCCATGGAAGCAATAAAGCTCTTCCCTGA
- refseq_ST3GAL3.F3 (forward primer)
- refseq_ST3GAL3.R3 (reverse primer)
- Alternative donor
Frame 0
TMAAPMQPSALWAPAGVPRGCRRRLRRCLRLPA
PRRAGRHLPPASLSAVVI*EIVNHVKMGLLVFVR
NLLLALCLFLVLGFLYYSAWKLHLLQWEEDSSK
YSHSSSPQEKPVADSVVLSFDSAGQTLGSEYDR
LGFLLNLDSKLSPRTLCTVVFGLDCILESPGEPK
KLLMPASHPLEILKSLSEDTAFALGFLKLPRPA
ELATKYANFSEGACKPGYASALMTAIFPRFSKP
APMFLDDSFRKWARIREFVPPFGIKGQDNLIKAI
LSVTKEYRLTPALDSLRCRRCIIVGNGGVLANK
SLGSRIDDYDIVVRLNSAPVKGFEKDVGSKTTL
RITYPEGAMQRPEQYERDSLFVLAGFKWQDFKWL
KYIVYKERVSASDGFWKSVATRVPKEPPEIRIL
NPYFIQEAAFTLIGLPFNNGLMGRGNIPTLGSV
AVTMALHGCDEVAVAGFGYDMSTPNAPLHYYETV
RMAAIKESWTHNIQREKEFLRKLVKARVITDLS
SGI*VGPAHGHRGPGTTRSSSQHHLHRSLQTQR
RTVPRAPGAARPWWSSQSCACSAASLRDQHSASF
SMGP*CQRASRLLAVPSRPSMQVVGHWAARLLP
ESLLQSVFGVLSFCEFG*GGG*G*FIFK*GWRC
QVGFTCHAGRGPLEGPSGSVTC*LPVGQECQDQP
VPCCGATGWWAGSQASPLQLMTLFGLSWGEGGV
FPLTSPSCPMGKPWSHPFGANKTAPGL*QKEL*
SSGG*RPAPPAGKSSPPQLQLIHRTTAGPDSPTC
HMF*VSATRLPGGWVLPLAWTKGK*GPRSLPKL
WQPSQATPMEAIKLFP
Frame 1
LWRRPCSPARCGLPPGSPAAVAAAYAAASAFLP
RVGPGATSPLPPSPLWSFRKS*IM*RWDSWYLC
AICC*PSASFWYWDFCIILRGSYTYSSGRRTPVS
IVTLAHPRRSLLQIQWFFPLTPLDKH*AQSMIG
WASS*IWTLNSHRGLSARWFLALTAYWNHLESL
KNY*CLHPTL*RF*SH*ARTQPLH*DF*SCPGLL
N*PPSTQTFQRELASLAMLQP**RPSSPGSPSQ
HPCSWMTPFASGLESGSSCRLLGSKVKTI*SKP
SCQSPKSTA*PLPWTASAAAAASSWAMEAFLPTS
LWGHELTTMTLW*D*IQHQ*KALRRTWAAKRHC
ASPTPRAPCSGLSSTSAILSLSSPASSGRTLSG
*NTSSTRRE*VHRMASGNLWPLECPRSPLRFESS
THISSRRPPSPSLACPSTMASWAGGTSLPLAVW
Q*PWHYTAVTRWQSQDLAMT*AHPTHPCTTMRP
FAWQPSKSPGRTISSERKSFCGSW*KLASSLI*A
VASEWAQHMAIEAQAPPGAAASTTYTGVFRPRE
GRCQGPQGQQGLGGAARAVPAQQPVSETSTQPH
SAWVLDARGPAGSWLCPAGPACRWWDTGQQGCCR
NHFSNQCLVYYHFVNLGRGEGRDNLFLNKVGDV
KLGSLAMQEEAH*RAHQAVLPVSSLWGRSARTS
LYLAVGLQDGGQDLKPAPSSS*HCLAFLGEKAGY
SHSPALAVPWGNPGAIPSEPTRPPQGYSRKNFK
AQEGDAQLRLLGRAPLHSCS*SIGLPQARTHQL
ATCSRFQQQDCQVVGFCL*PGPKGSEAQGAYPSC
GSRPRPPPWKQ*SSSL
Frame 2
YGGAHAAQRVVGSRRGPPRLSPPPTPLPPPSCP
ASGRAPPPPCLPLRCGHLGNRKSCEDGTLGICA
QSAASPLPLSGTGIFVLFCVEATLTPVGGGLQ*
V*SL*LTPGEACCRFSGSFL*LRWTNTRLRV*SV
GLPPESGL*TLTEDSLHGGFWP*LHIGITWRA*
KITDACIPPSRDFEVTERGHSLCIRIFKAAQAC
*ISHQVRKLFRGSLQAWLCFSLDDGHLPPVLQAS
THVPG*LLSQVG*NPGVRAAFWDQRSRQSDQSH
LVSHQRVPPDPCLGQPPLPPLHHRGQWRRSCQQ
VSGVTN*RL*HCGETEFSTSERL*EGRGQQNDTA
HHLPRGRHAAA*AVRARFSLCPRRLQVAGL*VV
EIHRLQGESECIGWLLEICGHSSAQGAP*DSNP
QPIFHPGGRLHPHWPALQQWPHGPGEHPYPWQCG
SDHGTTRL*RGGSRRIWL*HEHTQRTPALL*DR
SHGSHQRVLDAQYPARERVSAEAGESSRHH*SK
QWHLSGPSTWP*RPRHHQEQQPAPPTQESSDPEK
DGAKGPRGSKALVEQPELCLLSSQSQRPALSLI
QHGSLMPEGQQAPGCAQQAQHAGGGTLGSKAAA
GITSPISVWCIIIL*IWVGGRVGIIYF*IRLEMS
SWVHLPCRKRPTRGPIRQCYLLAPCGAGVPGPA
CTLLWGYRMVGRISSQPPPAHDTVWPFLGRRRG
IPTHQP*LSHGETLEPSLRSQQDRPRAIAERTLK
LRRVTPSSACWEELPSTAAADP*DYRRPGLTNL
PHVLGFSNKTARWLGSAFSLDQREVRPKELTQA
VAAVPGHPHGSNKALP*
Sequence NM_174965
ACTATGGCGGCGCCCATGCAGCCCAGCGCGTTGTGGGCTCCCGCCGGGGTCCCCCGCGGCTGTCGCCGCCGCCTACGCCGCTGCCTCCGCCTTCCTGCCC
CGCGTCGGGCCGGGCGCCACCTCCCCCCTGCCTCCCTCTCCGCTGTGGTCATTTAGGAAATCGTAAATCATGTGAAGATGGGACTCTTGGTATTTGTGCG
CAATCTGCTGCTAGCCCTCTGCCTCTTTCTGGTACTGGGATTTTTGTATTATTCTGCGTGGAAGCTACACTTACTCCAGTGGGAGGAGGACTCCAGTAAG
TATAGTCACTCTAGCTCACCCCAGGAGAAGCCTGTTGCAGATTCAGTGGTTCTTTCCTTTGACTCCGCTGGACAAACACTAGGCTCAGAGTATGATCGGT
TGGGCTTCCTCCTGAATCTGGACTCTAAACTGCCTGCTGAATTAGCCACCAAGTACGCAAACTTTTCAGAGGGAGCTTGCAAGCCTGGCTATGCTTCAGC
CTTGATGACGGCCATCTTCCCCCGGTTCTCCAAGCCAGCACCCATGTTCCTGGATGACTCCTTTCGCAAGTGGGCTAGAATCCGGGAGTTCGTGCCGCCT
TTTGGGATCAAAGGTCAAGTCCTGGACGCACAATATCCAGCGAGAGAAAGAGTTTCTGCGGAAGCTGGTGAAAGCTCGCGTCATCACTGATCTAAGCAGT
GGCATCTGAGTGGGCCCAGCACATGGCCATAGAGGCCCAGGCACCACCAGGAGCAGCAGCCAGCACCACCTACACAGGAGTCTTCAGACCCAGAGAAGGA
CGGTGCCAAGGGCCCCAGGGGCAGCAAGGCCTTGGTGGAGCAGCCAGAGCTGTGCCTGCTCAGCAGCCAGTCTCAGAGACCAGCACTCAGCCTCATTCAG
CATGGGTCCTTGATGCCAGAGGGCCAGCAGGCTCCTGGCTGTGCCCAGCAGGCCCAGCATGCAGGTGGTGGGACACTGGGCAGCAAGGCTGCTGCCGGAA
TCACTTCTCCAATCAGTGTTTGGTGTATTATCATTTTGTGAATTTGGGTAGGGGGGAGGGTAGGGATAATTTATTTTTAAATAAGGTTGGAGATGTCAAG
TTGGGTTCACTTGCCATGCAGGAAGAGGCCCACTAGAGGGCCCATCAGGCAGTGTTACCTGTTAGCTCCCTGTGGGGCAGGAGTGCCAGGACCAGCCTGT
ACCTTGCTGTGGGGCTACAGGATGGTGGGCAGGATCTCAAGCCAGCCCCCTCCAGCTCATGACACTGTTTGGCCTTTCTTGGGGAGAAGGCGGGGTATTC
CCACTCACCAGCCCTAGCTGTCCCATGGGGAAACCCTGGAGCCATCCCTTCGGAGCCAACAAGACCGCCCCAGGGCTATAGCAGAAAGAACTTTAAAGCT
CAGGAGGGTGACGCCCAGCTCCGCCTGCTGGGAAGAGCTCCCCTCCACAGCTGCAGCTGATCCATAGGACTACCGCAGGCCCGGACTCACCAACTTGCCA
CATGTTCTAGGTTTCAGCAACAAGACTGCCAGGTGGTTGGGTTCTGCCTTTAGCCTGGACCAAAGGGAAGTGAGGCCCAAGGAGCTTACCCAAGCTGTGG
CAGCCGTCCCAGGCCACCCCCATGGAAGCAATAAAGCTCTTCCCTGA
- refseq_ST3GAL3.F3 (forward primer)
- refseq_ST3GAL3.R3 (reverse primer)
- Alternative donor
Frame 0
TMAAPMQPSALWAPAGVPRGCRRRLRRCLRLPA
PRRAGRHLPPASLSAVVI*EIVNHVKMGLLVFVR
NLLLALCLFLVLGFLYYSAWKLHLLQWEEDSSK
YSHSSSPQEKPVADSVVLSFDSAGQTLGSEYDR
LGFLLNLDSKLPAELATKYANFSEGACKPGYASA
LMTAIFPRFSKPAPMFLDDSFRKWARIREFVPP
FGIKGQVLDAQYPARERVSAEAGESSRHH*SKQ
WHLSGPSTWP*RPRHHQEQQPAPPTQESSDPEKD
GAKGPRGSKALVEQPELCLLSSQSQRPALSLIQ
HGSLMPEGQQAPGCAQQAQHAGGGTLGSKAAAG
ITSPISVWCIIIL*IWVGGRVGIIYF*IRLEMSS
WVHLPCRKRPTRGPIRQCYLLAPCGAGVPGPAC
TLLWGYRMVGRISSQPPPAHDTVWPFLGRRRGI
PTHQP*LSHGETLEPSLRSQQDRPRAIAERTLKL
RRVTPSSACWEELPSTAAADP*DYRRPGLTNLP
HVLGFSNKTARWLGSAFSLDQREVRPKELTQAV
AAVPGHPHGSNKALP*
Frame 1
LWRRPCSPARCGLPPGSPAAVAAAYAAASAFLP
RVGPGATSPLPPSPLWSFRKS*IM*RWDSWYLC
AICC*PSASFWYWDFCIILRGSYTYSSGRRTPVS
IVTLAHPRRSLLQIQWFFPLTPLDKH*AQSMIG
WASS*IWTLNCLLN*PPSTQTFQRELASLAMLQ
P**RPSSPGSPSQHPCSWMTPFASGLESGSSCRL
LGSKVKSWTHNIQREKEFLRKLVKARVITDLSS
GI*VGPAHGHRGPGTTRSSSQHHLHRSLQTQRR
TVPRAPGAARPWWSSQSCACSAASLRDQHSASFS
MGP*CQRASRLLAVPSRPSMQVVGHWAARLLPE
SLLQSVFGVLSFCEFG*GGG*G*FIFK*GWRCQ
VGFTCHAGRGPLEGPSGSVTC*LPVGQECQDQPV
PCCGATGWWAGSQASPLQLMTLFGLSWGEGGVF
PLTSPSCPMGKPWSHPFGANKTAPGL*QKEL*S
SGG*RPAPPAGKSSPPQLQLIHRTTAGPDSPTCH
MF*VSATRLPGGWVLPLAWTKGK*GPRSLPKLW
QPSQATPMEAIKLFP
Frame 2
YGGAHAAQRVVGSRRGPPRLSPPPTPLPPPSCP
ASGRAPPPPCLPLRCGHLGNRKSCEDGTLGICA
QSAASPLPLSGTGIFVLFCVEATLTPVGGGLQ*
V*SL*LTPGEACCRFSGSFL*LRWTNTRLRV*SV
GLPPESGL*TAC*ISHQVRKLFRGSLQAWLCFS
LDDGHLPPVLQASTHVPG*LLSQVG*NPGVRAA
FWDQRSSPGRTISSERKSFCGSW*KLASSLI*AV
ASEWAQHMAIEAQAPPGAAASTTYTGVFRPREG
RCQGPQGQQGLGGAARAVPAQQPVSETSTQPHS
AWVLDARGPAGSWLCPAGPACRWWDTGQQGCCRN
HFSNQCLVYYHFVNLGRGEGRDNLFLNKVGDVK
LGSLAMQEEAH*RAHQAVLPVSSLWGRSARTSL
YLAVGLQDGGQDLKPAPSSS*HCLAFLGEKAGYS
HSPALAVPWGNPGAIPSEPTRPPQGYSRKNFKA
QEGDAQLRLLGRAPLHSCS*SIGLPQARTHQLA
TCSRFQQQDCQVVGFCL*PGPKGSEAQGAYPSCG
SRPRPPPWKQ*SSSL
Sequence NM_174964
ACTATGGCGGCGCCCATGCAGCCCAGCGCGTTGTGGGCTCCCGCCGGGGTCCCCCGCGGCTGTCGCCGCCGCCTACGCCGCTGCCTCCGCCTTCCTGCCC
CGCGTCGGGCCGGGCGCCACCTCCCCCCTGCCTCCCTCTCCGCTGTGGTCATTTAGGAAATCGTAAATCATGTGAAGATGGGACTCTTGGTATTTGTGCG
CAATCTGCTGCTAGCCCTCTGCCTCTTTCTGGTACTGGGATTTTTGTATTATTCTGCGTGGAAGCTACACTTACTCCAGTGGGAGGAGGACTCCAGTAAG
TATAGTCACTCTAGCTCACCCCAGGAGAAGCCTGTTGCAGATTCAGTGGTTCTTTCCTTTGACTCCGCTGGACAAACACTAGGCTCAGAGTATGATCGGT
TGGGCTTCCTCCTGAATCTGGACTCTAAACTGCCTGCTGAATTAGCCACCAAGTACGCAAACTTTTCAGAGGGAGCTTGCAAGCCTGGCTATGCTTCAGC
CTTGATGACGGCCATCTTCCCCCGGTTCTCCAAGCCAGCACCCATGTTCCTGGATGACTCCTTTCGCAAGTGGGCTAGAATCCGGGAGTTCGTGCCGCCT
TTTGGGATCAAAGGTCAAGACAATCTGATCAAAGCCATCTTGTCAGTCACCAAAGAGTACCGCCTGACCCCTGCCTTGGACAGCCTCCGCTGCCGCCGCT
GCATCATCGTGGGCAATGGAGGCGTTCTTGCCAACAAGTCTCTGGGGTCACGAATTGACGACTATGACATTGTGGTGAGACTGAATTCAGCACCAGTGAA
AGGCTTTGAGAAGGACGTGGGCAGCAAAACGACACTGCGCATCACCTACCCCGAGGGCGCCATGCAGCGGCCTGAGCAGTACGAGCGCGATTCTCTCTTT
GTCCTCGCCGGCTTCAAGTGGCAGGACTTTAAGTGGTTGAAATACATCGTCTACAAGGAGAGAGTGAGTGCATCGGATGGCTTCTGGAAATCTGTGGCCA
CTCGAGTGCCCAAGGAGCCCCCTGAGATTCGAATCCTCAACCCATATTTCATCCAGGAGGCCGCCTTCACCCTCATTGGCCTGCCCTTCAACAATGGCCT
CATGGGCCGGGGGAACATCCCTACCCTTGGCAGTGTGGCAGTGACCATGGCACTACACGGCTGTGACGAGGTGGCAGTCGCAGGATTTGGCTATGACATG
AGCACACCCAACGCACCCCTGCACTACTATGAGACCGTTCGCATGGCAGCCATCAAAGAGTCCTGGACGCACAATATCCAGCGAGAGAAAGAGTTTCTGC
GGAAGCTGGTGAAAGCTCGCGTCATCACTGATCTAAGCAGTGGCATCTGAGTGGGCCCAGCACATGGCCATAGAGGCCCAGGCACCACCAGGAGCAGCAG
CCAGCACCACCTACACAGGAGTCTTCAGACCCAGAGAAGGACGGTGCCAAGGGCCCCAGGGGCAGCAAGGCCTTGGTGGAGCAGCCAGAGCTGTGCCTGC
TCAGCAGCCAGTCTCAGAGACCAGCACTCAGCCTCATTCAGCATGGGTCCTTGATGCCAGAGGGCCAGCAGGCTCCTGGCTGTGCCCAGCAGGCCCAGCA
TGCAGGTGGTGGGACACTGGGCAGCAAGGCTGCTGCCGGAATCACTTCTCCAATCAGTGTTTGGTGTATTATCATTTTGTGAATTTGGGTAGGGGGGAGG
GTAGGGATAATTTATTTTTAAATAAGGTTGGAGATGTCAAGTTGGGTTCACTTGCCATGCAGGAAGAGGCCCACTAGAGGGCCCATCAGGCAGTGTTACC
TGTTAGCTCCCTGTGGGGCAGGAGTGCCAGGACCAGCCTGTACCTTGCTGTGGGGCTACAGGATGGTGGGCAGGATCTCAAGCCAGCCCCCTCCAGCTCA
TGACACTGTTTGGCCTTTCTTGGGGAGAAGGCGGGGTATTCCCACTCACCAGCCCTAGCTGTCCCATGGGGAAACCCTGGAGCCATCCCTTCGGAGCCAA
CAAGACCGCCCCAGGGCTATAGCAGAAAGAACTTTAAAGCTCAGGAGGGTGACGCCCAGCTCCGCCTGCTGGGAAGAGCTCCCCTCCACAGCTGCAGCTG
ATCCATAGGACTACCGCAGGCCCGGACTCACCAACTTGCCACATGTTCTAGGTTTCAGCAACAAGACTGCCAGGTGGTTGGGTTCTGCCTTTAGCCTGGA
CCAAAGGGAAGTGAGGCCCAAGGAGCTTACCCAAGCTGTGGCAGCCGTCCCAGGCCACCCCCATGGAAGCAATAAAGCTCTTCCCTGA
- refseq_ST3GAL3.F3 (forward primer)
- refseq_ST3GAL3.R3 (reverse primer)
- Alternative donor
Frame 0
TMAAPMQPSALWAPAGVPRGCRRRLRRCLRLPA
PRRAGRHLPPASLSAVVI*EIVNHVKMGLLVFVR
NLLLALCLFLVLGFLYYSAWKLHLLQWEEDSSK
YSHSSSPQEKPVADSVVLSFDSAGQTLGSEYDR
LGFLLNLDSKLPAELATKYANFSEGACKPGYASA
LMTAIFPRFSKPAPMFLDDSFRKWARIREFVPP
FGIKGQDNLIKAILSVTKEYRLTPALDSLRCRR
CIIVGNGGVLANKSLGSRIDDYDIVVRLNSAPVK
GFEKDVGSKTTLRITYPEGAMQRPEQYERDSLF
VLAGFKWQDFKWLKYIVYKERVSASDGFWKSVA
TRVPKEPPEIRILNPYFIQEAAFTLIGLPFNNGL
MGRGNIPTLGSVAVTMALHGCDEVAVAGFGYDM
STPNAPLHYYETVRMAAIKESWTHNIQREKEFL
RKLVKARVITDLSSGI*VGPAHGHRGPGTTRSSS
QHHLHRSLQTQRRTVPRAPGAARPWWSSQSCAC
SAASLRDQHSASFSMGP*CQRASRLLAVPSRPS
MQVVGHWAARLLPESLLQSVFGVLSFCEFG*GGG
*G*FIFK*GWRCQVGFTCHAGRGPLEGPSGSVT
C*LPVGQECQDQPVPCCGATGWWAGSQASPLQL
MTLFGLSWGEGGVFPLTSPSCPMGKPWSHPFGAN
KTAPGL*QKEL*SSGG*RPAPPAGKSSPPQLQL
IHRTTAGPDSPTCHMF*VSATRLPGGWVLPLAW
TKGK*GPRSLPKLWQPSQATPMEAIKLFP
Frame 1
LWRRPCSPARCGLPPGSPAAVAAAYAAASAFLP
RVGPGATSPLPPSPLWSFRKS*IM*RWDSWYLC
AICC*PSASFWYWDFCIILRGSYTYSSGRRTPVS
IVTLAHPRRSLLQIQWFFPLTPLDKH*AQSMIG
WASS*IWTLNCLLN*PPSTQTFQRELASLAMLQ
P**RPSSPGSPSQHPCSWMTPFASGLESGSSCRL
LGSKVKTI*SKPSCQSPKSTA*PLPWTASAAAA
ASSWAMEAFLPTSLWGHELTTMTLW*D*IQHQ*
KALRRTWAAKRHCASPTPRAPCSGLSSTSAILSL
SSPASSGRTLSG*NTSSTRRE*VHRMASGNLWP
LECPRSPLRFESSTHISSRRPPSPSLACPSTMA
SWAGGTSLPLAVWQ*PWHYTAVTRWQSQDLAMT*
AHPTHPCTTMRPFAWQPSKSPGRTISSERKSFC
GSW*KLASSLI*AVASEWAQHMAIEAQAPPGAA
ASTTYTGVFRPREGRCQGPQGQQGLGGAARAVPA
QQPVSETSTQPHSAWVLDARGPAGSWLCPAGPA
CRWWDTGQQGCCRNHFSNQCLVYYHFVNLGRGE
GRDNLFLNKVGDVKLGSLAMQEEAH*RAHQAVLP
VSSLWGRSARTSLYLAVGLQDGGQDLKPAPSSS
*HCLAFLGEKAGYSHSPALAVPWGNPGAIPSEP
TRPPQGYSRKNFKAQEGDAQLRLLGRAPLHSCS*
SIGLPQARTHQLATCSRFQQQDCQVVGFCL*PG
PKGSEAQGAYPSCGSRPRPPPWKQ*SSSL
Frame 2
YGGAHAAQRVVGSRRGPPRLSPPPTPLPPPSCP
ASGRAPPPPCLPLRCGHLGNRKSCEDGTLGICA
QSAASPLPLSGTGIFVLFCVEATLTPVGGGLQ*
V*SL*LTPGEACCRFSGSFL*LRWTNTRLRV*SV
GLPPESGL*TAC*ISHQVRKLFRGSLQAWLCFS
LDDGHLPPVLQASTHVPG*LLSQVG*NPGVRAA
FWDQRSRQSDQSHLVSHQRVPPDPCLGQPPLPPL
HHRGQWRRSCQQVSGVTN*RL*HCGETEFSTSE
RL*EGRGQQNDTAHHLPRGRHAAA*AVRARFSL
CPRRLQVAGL*VVEIHRLQGESECIGWLLEICGH
SSAQGAP*DSNPQPIFHPGGRLHPHWPALQQWP
HGPGEHPYPWQCGSDHGTTRL*RGGSRRIWL*H
EHTQRTPALL*DRSHGSHQRVLDAQYPARERVSA
EAGESSRHH*SKQWHLSGPSTWP*RPRHHQEQQ
PAPPTQESSDPEKDGAKGPRGSKALVEQPELCL
LSSQSQRPALSLIQHGSLMPEGQQAPGCAQQAQH
AGGGTLGSKAAAGITSPISVWCIIIL*IWVGGR
VGIIYF*IRLEMSSWVHLPCRKRPTRGPIRQCY
LLAPCGAGVPGPACTLLWGYRMVGRISSQPPPAH
DTVWPFLGRRRGIPTHQP*LSHGETLEPSLRSQ
QDRPRAIAERTLKLRRVTPSSACWEELPSTAAA
DP*DYRRPGLTNLPHVLGFSNKTARWLGSAFSLD
QREVRPKELTQAVAAVPGHPHGSNKALP*