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Sequences

Sequence NM_174967

refseq_ST3GAL3.F5 (forward primer)
refseq_ST3GAL3.R5 (reverse primer)
Single exon skipping

Frame 0

TMAAPMQPSALWAPAGVPRGCRRRLRRCLRLPA PRRAGRHLPPASLSAVVI*EIVNHVKMGLLVFVR NLLLALCLFLVLGFLYYSAWKLHLLQWEEDSNS VVLSFDSAGQTLGSEYDRLGFLLNLDSKLPAEL ATKYANFSEGACKPGYASALMTAIFPRFSKPAPM FLDDSFRKWARIREFVPPFGIKGQVLDAQYPAR ERVSAEAGESSRHH*SKQWHLSGPSTWP*RPRH HQEQQPAPPTQESSDPEKDGAKGPRGSKALVEQP ELCLLSSQSQRPALSLIQHGSLMPEGQQAPGCA QQAQHAGGGTLGSKAAAGITSPISVWCIIIL*I WVGGRVGIIYF*IRLEMSSWVHLPCRKRPTRGPI RQCYLLAPCGAGVPGPACTLLWGYRMVGRISSQ PPPAHDTVWPFLGRRRGIPTHQP*LSHGETLEP SLRSQQDRPRAIAERTLKLRRVTPSSACWEELPS TAAADP*DYRRPGLTNLPHVLGFSNKTARWLGS AFSLDQREVRPKELTQAVAAVPGHPHGSNKALP *

Frame 1

LWRRPCSPARCGLPPGSPAAVAAAYAAASAFLP RVGPGATSPLPPSPLWSFRKS*IM*RWDSWYLC AICC*PSASFWYWDFCIILRGSYTYSSGRRTPIQ WFFPLTPLDKH*AQSMIGWASS*IWTLNCLLN* PPSTQTFQRELASLAMLQP**RPSSPGSPSQHP CSWMTPFASGLESGSSCRLLGSKVKSWTHNIQRE KEFLRKLVKARVITDLSSGI*VGPAHGHRGPGT TRSSSQHHLHRSLQTQRRTVPRAPGAARPWWSS QSCACSAASLRDQHSASFSMGP*CQRASRLLAVP SRPSMQVVGHWAARLLPESLLQSVFGVLSFCEF G*GGG*G*FIFK*GWRCQVGFTCHAGRGPLEGP SGSVTC*LPVGQECQDQPVPCCGATGWWAGSQAS PLQLMTLFGLSWGEGGVFPLTSPSCPMGKPWSH PFGANKTAPGL*QKEL*SSGG*RPAPPAGKSSP PQLQLIHRTTAGPDSPTCHMF*VSATRLPGGWVL PLAWTKGK*GPRSLPKLWQPSQATPMEAIKLFP

Frame 2

YGGAHAAQRVVGSRRGPPRLSPPPTPLPPPSCP ASGRAPPPPCLPLRCGHLGNRKSCEDGTLGICA QSAASPLPLSGTGIFVLFCVEATLTPVGGGLQF SGSFL*LRWTNTRLRV*SVGLPPESGL*TAC*IS HQVRKLFRGSLQAWLCFSLDDGHLPPVLQASTH VPG*LLSQVG*NPGVRAAFWDQRSSPGRTISSE RKSFCGSW*KLASSLI*AVASEWAQHMAIEAQAP PGAAASTTYTGVFRPREGRCQGPQGQQGLGGAA RAVPAQQPVSETSTQPHSAWVLDARGPAGSWLC PAGPACRWWDTGQQGCCRNHFSNQCLVYYHFVNL GRGEGRDNLFLNKVGDVKLGSLAMQEEAH*RAH QAVLPVSSLWGRSARTSLYLAVGLQDGGQDLKP APSSS*HCLAFLGEKAGYSHSPALAVPWGNPGAI PSEPTRPPQGYSRKNFKAQEGDAQLRLLGRAPL HSCS*SIGLPQARTHQLATCSRFQQQDCQVVGF CL*PGPKGSEAQGAYPSCGSRPRPPPWKQ*SSSL

Sequence NM_174966

ACTATGGCGGCGCCCATGCAGCCCAGCGCGTTGTGGGCTCCCGCCGGGGTCCCCCGCGGCTGTCGCCGCCGCCTACGCCGCTGCCTCCGCCTTCCTGCCC CGCGTCGGGCCGGGCGCCACCTCCCCCCTGCCTCCCTCTCCGCTGTGGTCATTTAGGAAATCGTAAATCATGTGAAGATGGGACTCTTGGTATTTGTGCG CAATCTGCTGCTAGCCCTCTGCCTCTTTCTGGTACTGGGATTTTTGTATTATTCTGCGTGGAAGCTACACTTACTCCAGTGGGAGGAGGACTCCAATTCA GTGGTTCTTTCCTTTGACTCCGCTGGACAAACACTAGGCTCAGAGTATGATCGGTTGGGCTTCCTCCTGAATCTGGACTCTAAACTGCCTGCTGAATTAG CCACCAAGTACGCAAACTTTTCAGAGGGAGCTTGCAAGCCTGGCTATGCTTCAGCCTTGATGACGGCCATCTTCCCCCGGTTCTCCAAGCCAGCACCCAT GTTCCTGGATGACTCCTTTCGCAAGTGGGCTAGAATCCGGGAGTTCGTGCCGCCTTTTGGGATCAAAGGTCAAGACAATCTGATCAAAGCCATCTTGTCA GTCACCAAAGAGTACCGCCTGACCCCTGCCTTGGACAGCCTCCGCTGCCGCCGCTGCATCATCGTGGGCAATGGAGGCGTTCTTGCCAACAAGTCTCTGG GGTCACGAATTGACGACTATGACATTGTGGTGAGACTGAATTCAGCACCAGTGAAAGGCTTTGAGAAGGACGTGGGCAGCAAAACGACACTGCGCATCAC CTACCCCGAGGGCGCCATGCAGCGGCCTGAGCAGTACGAGCGCGATTCTCTCTTTGTCCTCGCCGGCTTCAAGTGGCAGGACTTTAAGTGGTTGAAATAC ATCGTCTACAAGGAGAGAGTGTCCTGGACGCACAATATCCAGCGAGAGAAAGAGTTTCTGCGGAAGCTGGTGAAAGCTCGCGTCATCACTGATCTAAGCA GTGGCATCTGAGTGGGCCCAGCACATGGCCATAGAGGCCCAGGCACCACCAGGAGCAGCAGCCAGCACCACCTACACAGGAGTCTTCAGACCCAGAGAAG GACGGTGCCAAGGGCCCCAGGGGCAGCAAGGCCTTGGTGGAGCAGCCAGAGCTGTGCCTGCTCAGCAGCCAGTCTCAGAGACCAGCACTCAGCCTCATTC AGCATGGGTCCTTGATGCCAGAGGGCCAGCAGGCTCCTGGCTGTGCCCAGCAGGCCCAGCATGCAGGTGGTGGGACACTGGGCAGCAAGGCTGCTGCCGG AATCACTTCTCCAATCAGTGTTTGGTGTATTATCATTTTGTGAATTTGGGTAGGGGGGAGGGTAGGGATAATTTATTTTTAAATAAGGTTGGAGATGTCA AGTTGGGTTCACTTGCCATGCAGGAAGAGGCCCACTAGAGGGCCCATCAGGCAGTGTTACCTGTTAGCTCCCTGTGGGGCAGGAGTGCCAGGACCAGCCT GTACCTTGCTGTGGGGCTACAGGATGGTGGGCAGGATCTCAAGCCAGCCCCCTCCAGCTCATGACACTGTTTGGCCTTTCTTGGGGAGAAGGCGGGGTAT TCCCACTCACCAGCCCTAGCTGTCCCATGGGGAAACCCTGGAGCCATCCCTTCGGAGCCAACAAGACCGCCCCAGGGCTATAGCAGAAAGAACTTTAAAG CTCAGGAGGGTGACGCCCAGCTCCGCCTGCTGGGAAGAGCTCCCCTCCACAGCTGCAGCTGATCCATAGGACTACCGCAGGCCCGGACTCACCAACTTGC CACATGTTCTAGGTTTCAGCAACAAGACTGCCAGGTGGTTGGGTTCTGCCTTTAGCCTGGACCAAAGGGAAGTGAGGCCCAAGGAGCTTACCCAAGCTGT GGCAGCCGTCCCAGGCCACCCCCATGGAAGCAATAAAGCTCTTCCCTGA

refseq_ST3GAL3.F5 (forward primer)
refseq_ST3GAL3.R5 (reverse primer)
Single exon skipping

Frame 0

TMAAPMQPSALWAPAGVPRGCRRRLRRCLRLPA PRRAGRHLPPASLSAVVI*EIVNHVKMGLLVFVR NLLLALCLFLVLGFLYYSAWKLHLLQWEEDSNS VVLSFDSAGQTLGSEYDRLGFLLNLDSKLPAEL ATKYANFSEGACKPGYASALMTAIFPRFSKPAPM FLDDSFRKWARIREFVPPFGIKGQDNLIKAILS VTKEYRLTPALDSLRCRRCIIVGNGGVLANKSL GSRIDDYDIVVRLNSAPVKGFEKDVGSKTTLRIT YPEGAMQRPEQYERDSLFVLAGFKWQDFKWLKY IVYKERVSWTHNIQREKEFLRKLVKARVITDLS SGI*VGPAHGHRGPGTTRSSSQHHLHRSLQTQRR TVPRAPGAARPWWSSQSCACSAASLRDQHSASF SMGP*CQRASRLLAVPSRPSMQVVGHWAARLLP ESLLQSVFGVLSFCEFG*GGG*G*FIFK*GWRCQ VGFTCHAGRGPLEGPSGSVTC*LPVGQECQDQP VPCCGATGWWAGSQASPLQLMTLFGLSWGEGGV FPLTSPSCPMGKPWSHPFGANKTAPGL*QKEL*S SGG*RPAPPAGKSSPPQLQLIHRTTAGPDSPTC HMF*VSATRLPGGWVLPLAWTKGK*GPRSLPKL WQPSQATPMEAIKLFP

Frame 1

LWRRPCSPARCGLPPGSPAAVAAAYAAASAFLP RVGPGATSPLPPSPLWSFRKS*IM*RWDSWYLC AICC*PSASFWYWDFCIILRGSYTYSSGRRTPIQ WFFPLTPLDKH*AQSMIGWASS*IWTLNCLLN* PPSTQTFQRELASLAMLQP**RPSSPGSPSQHP CSWMTPFASGLESGSSCRLLGSKVKTI*SKPSCQ SPKSTA*PLPWTASAAAAASSWAMEAFLPTSLW GHELTTMTLW*D*IQHQ*KALRRTWAAKRHCAS PTPRAPCSGLSSTSAILSLSSPASSGRTLSG*NT SSTRRECPGRTISSERKSFCGSW*KLASSLI*A VASEWAQHMAIEAQAPPGAAASTTYTGVFRPRE GRCQGPQGQQGLGGAARAVPAQQPVSETSTQPHS AWVLDARGPAGSWLCPAGPACRWWDTGQQGCCR NHFSNQCLVYYHFVNLGRGEGRDNLFLNKVGDV KLGSLAMQEEAH*RAHQAVLPVSSLWGRSARTSL YLAVGLQDGGQDLKPAPSSS*HCLAFLGEKAGY SHSPALAVPWGNPGAIPSEPTRPPQGYSRKNFK AQEGDAQLRLLGRAPLHSCS*SIGLPQARTHQLA TCSRFQQQDCQVVGFCL*PGPKGSEAQGAYPSC GSRPRPPPWKQ*SSSL

Frame 2

YGGAHAAQRVVGSRRGPPRLSPPPTPLPPPSCP ASGRAPPPPCLPLRCGHLGNRKSCEDGTLGICA QSAASPLPLSGTGIFVLFCVEATLTPVGGGLQF SGSFL*LRWTNTRLRV*SVGLPPESGL*TAC*IS HQVRKLFRGSLQAWLCFSLDDGHLPPVLQASTH VPG*LLSQVG*NPGVRAAFWDQRSRQSDQSHLV SHQRVPPDPCLGQPPLPPLHHRGQWRRSCQQVSG VTN*RL*HCGETEFSTSERL*EGRGQQNDTAHH LPRGRHAAA*AVRARFSLCPRRLQVAGL*VVEI HRLQGESVLDAQYPARERVSAEAGESSRHH*SKQ WHLSGPSTWP*RPRHHQEQQPAPPTQESSDPEK DGAKGPRGSKALVEQPELCLLSSQSQRPALSLI QHGSLMPEGQQAPGCAQQAQHAGGGTLGSKAAAG ITSPISVWCIIIL*IWVGGRVGIIYF*IRLEMS SWVHLPCRKRPTRGPIRQCYLLAPCGAGVPGPA CTLLWGYRMVGRISSQPPPAHDTVWPFLGRRRGI PTHQP*LSHGETLEPSLRSQQDRPRAIAERTLK LRRVTPSSACWEELPSTAAADP*DYRRPGLTNL PHVLGFSNKTARWLGSAFSLDQREVRPKELTQAV AAVPGHPHGSNKALP*

Sequence NM_006279

ACTATGGCGGCGCCCATGCAGCCCAGCGCGTTGTGGGCTCCCGCCGGGGTCCCCCGCGGCTGTCGCCGCCGCCTACGCCGCTGCCTCCGCCTTCCTGCCC CGCGTCGGGCCGGGCGCCACCTCCCCCCTGCCTCCCTCTCCGCTGTGGTCATTTAGGAAATCGTAAATCATGTGAAGATGGGACTCTTGGTATTTGTGCG CAATCTGCTGCTAGCCCTCTGCCTCTTTCTGGTACTGGGATTTTTGTATTATTCTGCGTGGAAGCTACACTTACTCCAGTGGGAGGAGGACTCCAATTCA GTGGTTCTTTCCTTTGACTCCGCTGGACAAACACTAGGCTCAGAGTATGATCGGTTGGGCTTCCTCCTGAATCTGGACTCTAAACTGCCTGCTGAATTAG CCACCAAGTACGCAAACTTTTCAGAGGGAGCTTGCAAGCCTGGCTATGCTTCAGCCTTGATGACGGCCATCTTCCCCCGGTTCTCCAAGCCAGCACCCAT GTTCCTGGATGACTCCTTTCGCAAGTGGGCTAGAATCCGGGAGTTCGTGCCGCCTTTTGGGATCAAAGGTCAAGACAATCTGATCAAAGCCATCTTGTCA GTCACCAAAGAGTACCGCCTGACCCCTGCCTTGGACAGCCTCCGCTGCCGCCGCTGCATCATCGTGGGCAATGGAGGCGTTCTTGCCAACAAGTCTCTGG GGTCACGAATTGACGACTATGACATTGTGGTGAGACTGAATTCAGCACCAGTGAAAGGCTTTGAGAAGGACGTGGGCAGCAAAACGACACTGCGCATCAC CTACCCCGAGGGCGCCATGCAGCGGCCTGAGCAGTACGAGCGCGATTCTCTCTTTGTCCTCGCCGGCTTCAAGTGGCAGGACTTTAAGTGGTTGAAATAC ATCGTCTACAAGGAGAGAGTGAGTGCATCGGATGGCTTCTGGAAATCTGTGGCCACTCGAGTGCCCAAGGAGCCCCCTGAGATTCGAATCCTCAACCCAT ATTTCATCCAGGAGGCCGCCTTCACCCTCATTGGCCTGCCCTTCAACAATGGCCTCATGGGCCGGGGGAACATCCCTACCCTTGGCAGTGTGGCAGTGAC CATGGCACTACACGGCTGTGACGAGGTGGCAGTCGCAGGATTTGGCTATGACATGAGCACACCCAACGCACCCCTGCACTACTATGAGACCGTTCGCATG GCAGCCATCAAAGAGTCCTGGACGCACAATATCCAGCGAGAGAAAGAGTTTCTGCGGAAGCTGGTGAAAGCTCGCGTCATCACTGATCTAAGCAGTGGCA TCTGAGTGGGCCCAGCACATGGCCATAGAGGCCCAGGCACCACCAGGAGCAGCAGCCAGCACCACCTACACAGGAGTCTTCAGACCCAGAGAAGGACGGT GCCAAGGGCCCCAGGGGCAGCAAGGCCTTGGTGGAGCAGCCAGAGCTGTGCCTGCTCAGCAGCCAGTCTCAGAGACCAGCACTCAGCCTCATTCAGCATG GGTCCTTGATGCCAGAGGGCCAGCAGGCTCCTGGCTGTGCCCAGCAGGCCCAGCATGCAGGTGGTGGGACACTGGGCAGCAAGGCTGCTGCCGGAATCAC TTCTCCAATCAGTGTTTGGTGTATTATCATTTTGTGAATTTGGGTAGGGGGGAGGGTAGGGATAATTTATTTTTAAATAAGGTTGGAGATGTCAAGTTGG GTTCACTTGCCATGCAGGAAGAGGCCCACTAGAGGGCCCATCAGGCAGTGTTACCTGTTAGCTCCCTGTGGGGCAGGAGTGCCAGGACCAGCCTGTACCT TGCTGTGGGGCTACAGGATGGTGGGCAGGATCTCAAGCCAGCCCCCTCCAGCTCATGACACTGTTTGGCCTTTCTTGGGGAGAAGGCGGGGTATTCCCAC TCACCAGCCCTAGCTGTCCCATGGGGAAACCCTGGAGCCATCCCTTCGGAGCCAACAAGACCGCCCCAGGGCTATAGCAGAAAGAACTTTAAAGCTCAGG AGGGTGACGCCCAGCTCCGCCTGCTGGGAAGAGCTCCCCTCCACAGCTGCAGCTGATCCATAGGACTACCGCAGGCCCGGACTCACCAACTTGCCACATG TTCTAGGTTTCAGCAACAAGACTGCCAGGTGGTTGGGTTCTGCCTTTAGCCTGGACCAAAGGGAAGTGAGGCCCAAGGAGCTTACCCAAGCTGTGGCAGC CGTCCCAGGCCACCCCCATGGAAGCAATAAAGCTCTTCCCTGA

refseq_ST3GAL3.F5 (forward primer)
refseq_ST3GAL3.R5 (reverse primer)
Single exon skipping

Frame 0

TMAAPMQPSALWAPAGVPRGCRRRLRRCLRLPA PRRAGRHLPPASLSAVVI*EIVNHVKMGLLVFVR NLLLALCLFLVLGFLYYSAWKLHLLQWEEDSNS VVLSFDSAGQTLGSEYDRLGFLLNLDSKLPAEL ATKYANFSEGACKPGYASALMTAIFPRFSKPAPM FLDDSFRKWARIREFVPPFGIKGQDNLIKAILS VTKEYRLTPALDSLRCRRCIIVGNGGVLANKSL GSRIDDYDIVVRLNSAPVKGFEKDVGSKTTLRIT YPEGAMQRPEQYERDSLFVLAGFKWQDFKWLKY IVYKERVSASDGFWKSVATRVPKEPPEIRILNP YFIQEAAFTLIGLPFNNGLMGRGNIPTLGSVAVT MALHGCDEVAVAGFGYDMSTPNAPLHYYETVRM AAIKESWTHNIQREKEFLRKLVKARVITDLSSG I*VGPAHGHRGPGTTRSSSQHHLHRSLQTQRRTV PRAPGAARPWWSSQSCACSAASLRDQHSASFSM GP*CQRASRLLAVPSRPSMQVVGHWAARLLPES LLQSVFGVLSFCEFG*GGG*G*FIFK*GWRCQVG FTCHAGRGPLEGPSGSVTC*LPVGQECQDQPVP CCGATGWWAGSQASPLQLMTLFGLSWGEGGVFP LTSPSCPMGKPWSHPFGANKTAPGL*QKEL*SSG G*RPAPPAGKSSPPQLQLIHRTTAGPDSPTCHM F*VSATRLPGGWVLPLAWTKGK*GPRSLPKLWQ PSQATPMEAIKLFP

Frame 1

LWRRPCSPARCGLPPGSPAAVAAAYAAASAFLP RVGPGATSPLPPSPLWSFRKS*IM*RWDSWYLC AICC*PSASFWYWDFCIILRGSYTYSSGRRTPIQ WFFPLTPLDKH*AQSMIGWASS*IWTLNCLLN* PPSTQTFQRELASLAMLQP**RPSSPGSPSQHP CSWMTPFASGLESGSSCRLLGSKVKTI*SKPSCQ SPKSTA*PLPWTASAAAAASSWAMEAFLPTSLW GHELTTMTLW*D*IQHQ*KALRRTWAAKRHCAS PTPRAPCSGLSSTSAILSLSSPASSGRTLSG*NT SSTRRE*VHRMASGNLWPLECPRSPLRFESSTH ISSRRPPSPSLACPSTMASWAGGTSLPLAVWQ* PWHYTAVTRWQSQDLAMT*AHPTHPCTTMRPFAW QPSKSPGRTISSERKSFCGSW*KLASSLI*AVA SEWAQHMAIEAQAPPGAAASTTYTGVFRPREGR CQGPQGQQGLGGAARAVPAQQPVSETSTQPHSAW VLDARGPAGSWLCPAGPACRWWDTGQQGCCRNH FSNQCLVYYHFVNLGRGEGRDNLFLNKVGDVKL GSLAMQEEAH*RAHQAVLPVSSLWGRSARTSLYL AVGLQDGGQDLKPAPSSS*HCLAFLGEKAGYSH SPALAVPWGNPGAIPSEPTRPPQGYSRKNFKAQ EGDAQLRLLGRAPLHSCS*SIGLPQARTHQLATC SRFQQQDCQVVGFCL*PGPKGSEAQGAYPSCGS RPRPPPWKQ*SSSL

Frame 2

YGGAHAAQRVVGSRRGPPRLSPPPTPLPPPSCP ASGRAPPPPCLPLRCGHLGNRKSCEDGTLGICA QSAASPLPLSGTGIFVLFCVEATLTPVGGGLQF SGSFL*LRWTNTRLRV*SVGLPPESGL*TAC*IS HQVRKLFRGSLQAWLCFSLDDGHLPPVLQASTH VPG*LLSQVG*NPGVRAAFWDQRSRQSDQSHLV SHQRVPPDPCLGQPPLPPLHHRGQWRRSCQQVSG VTN*RL*HCGETEFSTSERL*EGRGQQNDTAHH LPRGRHAAA*AVRARFSLCPRRLQVAGL*VVEI HRLQGESECIGWLLEICGHSSAQGAP*DSNPQPI FHPGGRLHPHWPALQQWPHGPGEHPYPWQCGSD HGTTRL*RGGSRRIWL*HEHTQRTPALL*DRSH GSHQRVLDAQYPARERVSAEAGESSRHH*SKQWH LSGPSTWP*RPRHHQEQQPAPPTQESSDPEKDG AKGPRGSKALVEQPELCLLSSQSQRPALSLIQH GSLMPEGQQAPGCAQQAQHAGGGTLGSKAAAGIT SPISVWCIIIL*IWVGGRVGIIYF*IRLEMSSW VHLPCRKRPTRGPIRQCYLLAPCGAGVPGPACT LLWGYRMVGRISSQPPPAHDTVWPFLGRRRGIPT HQP*LSHGETLEPSLRSQQDRPRAIAERTLKLR RVTPSSACWEELPSTAAADP*DYRRPGLTNLPH VLGFSNKTARWLGSAFSLDQREVRPKELTQAVAA VPGHPHGSNKALP*

Sequence NM_174965

refseq_ST3GAL3.F5 (forward primer)
refseq_ST3GAL3.R5 (reverse primer)
Single exon skipping

Frame 0

TMAAPMQPSALWAPAGVPRGCRRRLRRCLRLPA PRRAGRHLPPASLSAVVI*EIVNHVKMGLLVFVR NLLLALCLFLVLGFLYYSAWKLHLLQWEEDSSK YSHSSSPQEKPVADSVVLSFDSAGQTLGSEYDR LGFLLNLDSKLPAELATKYANFSEGACKPGYASA LMTAIFPRFSKPAPMFLDDSFRKWARIREFVPP FGIKGQVLDAQYPARERVSAEAGESSRHH*SKQ WHLSGPSTWP*RPRHHQEQQPAPPTQESSDPEKD GAKGPRGSKALVEQPELCLLSSQSQRPALSLIQ HGSLMPEGQQAPGCAQQAQHAGGGTLGSKAAAG ITSPISVWCIIIL*IWVGGRVGIIYF*IRLEMSS WVHLPCRKRPTRGPIRQCYLLAPCGAGVPGPAC TLLWGYRMVGRISSQPPPAHDTVWPFLGRRRGI PTHQP*LSHGETLEPSLRSQQDRPRAIAERTLKL RRVTPSSACWEELPSTAAADP*DYRRPGLTNLP HVLGFSNKTARWLGSAFSLDQREVRPKELTQAV AAVPGHPHGSNKALP*

Frame 1

LWRRPCSPARCGLPPGSPAAVAAAYAAASAFLP RVGPGATSPLPPSPLWSFRKS*IM*RWDSWYLC AICC*PSASFWYWDFCIILRGSYTYSSGRRTPVS IVTLAHPRRSLLQIQWFFPLTPLDKH*AQSMIG WASS*IWTLNCLLN*PPSTQTFQRELASLAMLQ P**RPSSPGSPSQHPCSWMTPFASGLESGSSCRL LGSKVKSWTHNIQREKEFLRKLVKARVITDLSS GI*VGPAHGHRGPGTTRSSSQHHLHRSLQTQRR TVPRAPGAARPWWSSQSCACSAASLRDQHSASFS MGP*CQRASRLLAVPSRPSMQVVGHWAARLLPE SLLQSVFGVLSFCEFG*GGG*G*FIFK*GWRCQ VGFTCHAGRGPLEGPSGSVTC*LPVGQECQDQPV PCCGATGWWAGSQASPLQLMTLFGLSWGEGGVF PLTSPSCPMGKPWSHPFGANKTAPGL*QKEL*S SGG*RPAPPAGKSSPPQLQLIHRTTAGPDSPTCH MF*VSATRLPGGWVLPLAWTKGK*GPRSLPKLW QPSQATPMEAIKLFP

Frame 2

YGGAHAAQRVVGSRRGPPRLSPPPTPLPPPSCP ASGRAPPPPCLPLRCGHLGNRKSCEDGTLGICA QSAASPLPLSGTGIFVLFCVEATLTPVGGGLQ* V*SL*LTPGEACCRFSGSFL*LRWTNTRLRV*SV GLPPESGL*TAC*ISHQVRKLFRGSLQAWLCFS LDDGHLPPVLQASTHVPG*LLSQVG*NPGVRAA FWDQRSSPGRTISSERKSFCGSW*KLASSLI*AV ASEWAQHMAIEAQAPPGAAASTTYTGVFRPREG RCQGPQGQQGLGGAARAVPAQQPVSETSTQPHS AWVLDARGPAGSWLCPAGPACRWWDTGQQGCCRN HFSNQCLVYYHFVNLGRGEGRDNLFLNKVGDVK LGSLAMQEEAH*RAHQAVLPVSSLWGRSARTSL YLAVGLQDGGQDLKPAPSSS*HCLAFLGEKAGYS HSPALAVPWGNPGAIPSEPTRPPQGYSRKNFKA QEGDAQLRLLGRAPLHSCS*SIGLPQARTHQLA TCSRFQQQDCQVVGFCL*PGPKGSEAQGAYPSCG SRPRPPPWKQ*SSSL

Sequence NM_174964

ACTATGGCGGCGCCCATGCAGCCCAGCGCGTTGTGGGCTCCCGCCGGGGTCCCCCGCGGCTGTCGCCGCCGCCTACGCCGCTGCCTCCGCCTTCCTGCCC CGCGTCGGGCCGGGCGCCACCTCCCCCCTGCCTCCCTCTCCGCTGTGGTCATTTAGGAAATCGTAAATCATGTGAAGATGGGACTCTTGGTATTTGTGCG CAATCTGCTGCTAGCCCTCTGCCTCTTTCTGGTACTGGGATTTTTGTATTATTCTGCGTGGAAGCTACACTTACTCCAGTGGGAGGAGGACTCCAGTAAG TATAGTCACTCTAGCTCACCCCAGGAGAAGCCTGTTGCAGATTCAGTGGTTCTTTCCTTTGACTCCGCTGGACAAACACTAGGCTCAGAGTATGATCGGT TGGGCTTCCTCCTGAATCTGGACTCTAAACTGCCTGCTGAATTAGCCACCAAGTACGCAAACTTTTCAGAGGGAGCTTGCAAGCCTGGCTATGCTTCAGC CTTGATGACGGCCATCTTCCCCCGGTTCTCCAAGCCAGCACCCATGTTCCTGGATGACTCCTTTCGCAAGTGGGCTAGAATCCGGGAGTTCGTGCCGCCT TTTGGGATCAAAGGTCAAGACAATCTGATCAAAGCCATCTTGTCAGTCACCAAAGAGTACCGCCTGACCCCTGCCTTGGACAGCCTCCGCTGCCGCCGCT GCATCATCGTGGGCAATGGAGGCGTTCTTGCCAACAAGTCTCTGGGGTCACGAATTGACGACTATGACATTGTGGTGAGACTGAATTCAGCACCAGTGAA AGGCTTTGAGAAGGACGTGGGCAGCAAAACGACACTGCGCATCACCTACCCCGAGGGCGCCATGCAGCGGCCTGAGCAGTACGAGCGCGATTCTCTCTTT GTCCTCGCCGGCTTCAAGTGGCAGGACTTTAAGTGGTTGAAATACATCGTCTACAAGGAGAGAGTGAGTGCATCGGATGGCTTCTGGAAATCTGTGGCCA CTCGAGTGCCCAAGGAGCCCCCTGAGATTCGAATCCTCAACCCATATTTCATCCAGGAGGCCGCCTTCACCCTCATTGGCCTGCCCTTCAACAATGGCCT CATGGGCCGGGGGAACATCCCTACCCTTGGCAGTGTGGCAGTGACCATGGCACTACACGGCTGTGACGAGGTGGCAGTCGCAGGATTTGGCTATGACATG AGCACACCCAACGCACCCCTGCACTACTATGAGACCGTTCGCATGGCAGCCATCAAAGAGTCCTGGACGCACAATATCCAGCGAGAGAAAGAGTTTCTGC GGAAGCTGGTGAAAGCTCGCGTCATCACTGATCTAAGCAGTGGCATCTGAGTGGGCCCAGCACATGGCCATAGAGGCCCAGGCACCACCAGGAGCAGCAG CCAGCACCACCTACACAGGAGTCTTCAGACCCAGAGAAGGACGGTGCCAAGGGCCCCAGGGGCAGCAAGGCCTTGGTGGAGCAGCCAGAGCTGTGCCTGC TCAGCAGCCAGTCTCAGAGACCAGCACTCAGCCTCATTCAGCATGGGTCCTTGATGCCAGAGGGCCAGCAGGCTCCTGGCTGTGCCCAGCAGGCCCAGCA TGCAGGTGGTGGGACACTGGGCAGCAAGGCTGCTGCCGGAATCACTTCTCCAATCAGTGTTTGGTGTATTATCATTTTGTGAATTTGGGTAGGGGGGAGG GTAGGGATAATTTATTTTTAAATAAGGTTGGAGATGTCAAGTTGGGTTCACTTGCCATGCAGGAAGAGGCCCACTAGAGGGCCCATCAGGCAGTGTTACC TGTTAGCTCCCTGTGGGGCAGGAGTGCCAGGACCAGCCTGTACCTTGCTGTGGGGCTACAGGATGGTGGGCAGGATCTCAAGCCAGCCCCCTCCAGCTCA TGACACTGTTTGGCCTTTCTTGGGGAGAAGGCGGGGTATTCCCACTCACCAGCCCTAGCTGTCCCATGGGGAAACCCTGGAGCCATCCCTTCGGAGCCAA CAAGACCGCCCCAGGGCTATAGCAGAAAGAACTTTAAAGCTCAGGAGGGTGACGCCCAGCTCCGCCTGCTGGGAAGAGCTCCCCTCCACAGCTGCAGCTG ATCCATAGGACTACCGCAGGCCCGGACTCACCAACTTGCCACATGTTCTAGGTTTCAGCAACAAGACTGCCAGGTGGTTGGGTTCTGCCTTTAGCCTGGA CCAAAGGGAAGTGAGGCCCAAGGAGCTTACCCAAGCTGTGGCAGCCGTCCCAGGCCACCCCCATGGAAGCAATAAAGCTCTTCCCTGA

refseq_ST3GAL3.F5 (forward primer)
refseq_ST3GAL3.R5 (reverse primer)
Single exon skipping

Frame 0

TMAAPMQPSALWAPAGVPRGCRRRLRRCLRLPA PRRAGRHLPPASLSAVVI*EIVNHVKMGLLVFVR NLLLALCLFLVLGFLYYSAWKLHLLQWEEDSSK YSHSSSPQEKPVADSVVLSFDSAGQTLGSEYDR LGFLLNLDSKLPAELATKYANFSEGACKPGYASA LMTAIFPRFSKPAPMFLDDSFRKWARIREFVPP FGIKGQDNLIKAILSVTKEYRLTPALDSLRCRR CIIVGNGGVLANKSLGSRIDDYDIVVRLNSAPVK GFEKDVGSKTTLRITYPEGAMQRPEQYERDSLF VLAGFKWQDFKWLKYIVYKERVSASDGFWKSVA TRVPKEPPEIRILNPYFIQEAAFTLIGLPFNNGL MGRGNIPTLGSVAVTMALHGCDEVAVAGFGYDM STPNAPLHYYETVRMAAIKESWTHNIQREKEFL RKLVKARVITDLSSGI*VGPAHGHRGPGTTRSSS QHHLHRSLQTQRRTVPRAPGAARPWWSSQSCAC SAASLRDQHSASFSMGP*CQRASRLLAVPSRPS MQVVGHWAARLLPESLLQSVFGVLSFCEFG*GGG *G*FIFK*GWRCQVGFTCHAGRGPLEGPSGSVT C*LPVGQECQDQPVPCCGATGWWAGSQASPLQL MTLFGLSWGEGGVFPLTSPSCPMGKPWSHPFGAN KTAPGL*QKEL*SSGG*RPAPPAGKSSPPQLQL IHRTTAGPDSPTCHMF*VSATRLPGGWVLPLAW TKGK*GPRSLPKLWQPSQATPMEAIKLFP

Frame 1

LWRRPCSPARCGLPPGSPAAVAAAYAAASAFLP RVGPGATSPLPPSPLWSFRKS*IM*RWDSWYLC AICC*PSASFWYWDFCIILRGSYTYSSGRRTPVS IVTLAHPRRSLLQIQWFFPLTPLDKH*AQSMIG WASS*IWTLNCLLN*PPSTQTFQRELASLAMLQ P**RPSSPGSPSQHPCSWMTPFASGLESGSSCRL LGSKVKTI*SKPSCQSPKSTA*PLPWTASAAAA ASSWAMEAFLPTSLWGHELTTMTLW*D*IQHQ* KALRRTWAAKRHCASPTPRAPCSGLSSTSAILSL SSPASSGRTLSG*NTSSTRRE*VHRMASGNLWP LECPRSPLRFESSTHISSRRPPSPSLACPSTMA SWAGGTSLPLAVWQ*PWHYTAVTRWQSQDLAMT* AHPTHPCTTMRPFAWQPSKSPGRTISSERKSFC GSW*KLASSLI*AVASEWAQHMAIEAQAPPGAA ASTTYTGVFRPREGRCQGPQGQQGLGGAARAVPA QQPVSETSTQPHSAWVLDARGPAGSWLCPAGPA CRWWDTGQQGCCRNHFSNQCLVYYHFVNLGRGE GRDNLFLNKVGDVKLGSLAMQEEAH*RAHQAVLP VSSLWGRSARTSLYLAVGLQDGGQDLKPAPSSS *HCLAFLGEKAGYSHSPALAVPWGNPGAIPSEP TRPPQGYSRKNFKAQEGDAQLRLLGRAPLHSCS* SIGLPQARTHQLATCSRFQQQDCQVVGFCL*PG PKGSEAQGAYPSCGSRPRPPPWKQ*SSSL

Frame 2

YGGAHAAQRVVGSRRGPPRLSPPPTPLPPPSCP ASGRAPPPPCLPLRCGHLGNRKSCEDGTLGICA QSAASPLPLSGTGIFVLFCVEATLTPVGGGLQ* V*SL*LTPGEACCRFSGSFL*LRWTNTRLRV*SV GLPPESGL*TAC*ISHQVRKLFRGSLQAWLCFS LDDGHLPPVLQASTHVPG*LLSQVG*NPGVRAA FWDQRSRQSDQSHLVSHQRVPPDPCLGQPPLPPL HHRGQWRRSCQQVSGVTN*RL*HCGETEFSTSE RL*EGRGQQNDTAHHLPRGRHAAA*AVRARFSL CPRRLQVAGL*VVEIHRLQGESECIGWLLEICGH SSAQGAP*DSNPQPIFHPGGRLHPHWPALQQWP HGPGEHPYPWQCGSDHGTTRL*RGGSRRIWL*H EHTQRTPALL*DRSHGSHQRVLDAQYPARERVSA EAGESSRHH*SKQWHLSGPSTWP*RPRHHQEQQ PAPPTQESSDPEKDGAKGPRGSKALVEQPELCL LSSQSQRPALSLIQHGSLMPEGQQAPGCAQQAQH AGGGTLGSKAAAGITSPISVWCIIIL*IWVGGR VGIIYF*IRLEMSSWVHLPCRKRPTRGPIRQCY LLAPCGAGVPGPACTLLWGYRMVGRISSQPPPAH DTVWPFLGRRRGIPTHQP*LSHGETLEPSLRSQ QDRPRAIAERTLKLRRVTPSSACWEELPSTAAA DP*DYRRPGLTNLPHVLGFSNKTARWLGSAFSLD QREVRPKELTQAVAAVPGHPHGSNKALP*

Sequence NM_174963

ACTATGGCGGCGCCCATGCAGCCCAGCGCGTTGTGGGCTCCCGCCGGGGTCCCCCGCGGCTGTCGCCGCCGCCTACGCCGCTGCCTCCGCCTTCCTGCCC CGCGTCGGGCCGGGCGCCACCTCCCCCCTGCCTCCCTCTCCGCTGTGGTCATTTAGGAAATCGTAAATCATGTGAAGATGGGACTCTTGGTATTTGTGCG CAATCTGCTGCTAGCCCTCTGCCTCTTTCTGGTACTGGGATTTTTGTATTATTCTGCGTGGAAGCTACACTTACTCCAGTGGGAGGAGGACTCCAGTAAG TATAGTCACTCTAGCTCACCCCAGGAGAAGCCTGTTGCAGATTCAGTGGTTCTTTCCTTTGACTCCGCTGGACAAACACTAGGCTCAGAGTATGATCGGT TGGGCTTCCTCCTGAATCTGGACTCTAAACTCTCACCGAGGACTCTCTGCACGGTGGTTTTTGGCCTTGACTGCATATTGGAATCACCTGGAGAGCCTAA AAAATTACTGATGCCTGCATCCCACCCTCTAGAGATTTTGAAGTCACTGAGCGAGGACACAGCCTTTGCATTAGGATTTTTAAAGCTGCCCAGGCCTGCT GAATTAGCCACCAAGTACGCAAACTTTTCAGAGGGAGCTTGCAAGCCTGGCTATGCTTCAGCCTTGATGACGGCCATCTTCCCCCGGTTCTCCAAGCCAG CACCCATGTTCCTGGATGACTCCTTTCGCAAGTGGGCTAGAATCCGGGAGTTCGTGCCGCCTTTTGGGATCAAAGGTCAAGACAATCTGATCAAAGCCAT CTTGTCAGTCACCAAAGAGTACCGCCTGACCCCTGCCTTGGACAGCCTCCGCTGCCGCCGCTGCATCATCGTGGGCAATGGAGGCGTTCTTGCCAACAAG TCTCTGGGGTCACGAATTGACGACTATGACATTGTGGTGAGACTGAATTCAGCACCAGTGAAAGGCTTTGAGAAGGACGTGGGCAGCAAAACGACACTGC GCATCACCTACCCCGAGGGCGCCATGCAGCGGCCTGAGCAGTACGAGCGCGATTCTCTCTTTGTCCTCGCCGGCTTCAAGTGGCAGGACTTTAAGTGGTT GAAATACATCGTCTACAAGGAGAGAGTGAGTGCATCGGATGGCTTCTGGAAATCTGTGGCCACTCGAGTGCCCAAGGAGCCCCCTGAGATTCGAATCCTC AACCCATATTTCATCCAGGAGGCCGCCTTCACCCTCATTGGCCTGCCCTTCAACAATGGCCTCATGGGCCGGGGGAACATCCCTACCCTTGGCAGTGTGG CAGTGACCATGGCACTACACGGCTGTGACGAGGTGGCAGTCGCAGGATTTGGCTATGACATGAGCACACCCAACGCACCCCTGCACTACTATGAGACCGT TCGCATGGCAGCCATCAAAGAGTCCTGGACGCACAATATCCAGCGAGAGAAAGAGTTTCTGCGGAAGCTGGTGAAAGCTCGCGTCATCACTGATCTAAGC AGTGGCATCTGAGTGGGCCCAGCACATGGCCATAGAGGCCCAGGCACCACCAGGAGCAGCAGCCAGCACCACCTACACAGGAGTCTTCAGACCCAGAGAA GGACGGTGCCAAGGGCCCCAGGGGCAGCAAGGCCTTGGTGGAGCAGCCAGAGCTGTGCCTGCTCAGCAGCCAGTCTCAGAGACCAGCACTCAGCCTCATT CAGCATGGGTCCTTGATGCCAGAGGGCCAGCAGGCTCCTGGCTGTGCCCAGCAGGCCCAGCATGCAGGTGGTGGGACACTGGGCAGCAAGGCTGCTGCCG GAATCACTTCTCCAATCAGTGTTTGGTGTATTATCATTTTGTGAATTTGGGTAGGGGGGAGGGTAGGGATAATTTATTTTTAAATAAGGTTGGAGATGTC AAGTTGGGTTCACTTGCCATGCAGGAAGAGGCCCACTAGAGGGCCCATCAGGCAGTGTTACCTGTTAGCTCCCTGTGGGGCAGGAGTGCCAGGACCAGCC TGTACCTTGCTGTGGGGCTACAGGATGGTGGGCAGGATCTCAAGCCAGCCCCCTCCAGCTCATGACACTGTTTGGCCTTTCTTGGGGAGAAGGCGGGGTA TTCCCACTCACCAGCCCTAGCTGTCCCATGGGGAAACCCTGGAGCCATCCCTTCGGAGCCAACAAGACCGCCCCAGGGCTATAGCAGAAAGAACTTTAAA GCTCAGGAGGGTGACGCCCAGCTCCGCCTGCTGGGAAGAGCTCCCCTCCACAGCTGCAGCTGATCCATAGGACTACCGCAGGCCCGGACTCACCAACTTG CCACATGTTCTAGGTTTCAGCAACAAGACTGCCAGGTGGTTGGGTTCTGCCTTTAGCCTGGACCAAAGGGAAGTGAGGCCCAAGGAGCTTACCCAAGCTG TGGCAGCCGTCCCAGGCCACCCCCATGGAAGCAATAAAGCTCTTCCCTGA

refseq_ST3GAL3.F5 (forward primer)
refseq_ST3GAL3.R5 (reverse primer)
Single exon skipping

Frame 0

TMAAPMQPSALWAPAGVPRGCRRRLRRCLRLPA PRRAGRHLPPASLSAVVI*EIVNHVKMGLLVFVR NLLLALCLFLVLGFLYYSAWKLHLLQWEEDSSK YSHSSSPQEKPVADSVVLSFDSAGQTLGSEYDR LGFLLNLDSKLSPRTLCTVVFGLDCILESPGEPK KLLMPASHPLEILKSLSEDTAFALGFLKLPRPA ELATKYANFSEGACKPGYASALMTAIFPRFSKP APMFLDDSFRKWARIREFVPPFGIKGQDNLIKAI LSVTKEYRLTPALDSLRCRRCIIVGNGGVLANK SLGSRIDDYDIVVRLNSAPVKGFEKDVGSKTTL RITYPEGAMQRPEQYERDSLFVLAGFKWQDFKWL KYIVYKERVSASDGFWKSVATRVPKEPPEIRIL NPYFIQEAAFTLIGLPFNNGLMGRGNIPTLGSV AVTMALHGCDEVAVAGFGYDMSTPNAPLHYYETV RMAAIKESWTHNIQREKEFLRKLVKARVITDLS SGI*VGPAHGHRGPGTTRSSSQHHLHRSLQTQR RTVPRAPGAARPWWSSQSCACSAASLRDQHSASF SMGP*CQRASRLLAVPSRPSMQVVGHWAARLLP ESLLQSVFGVLSFCEFG*GGG*G*FIFK*GWRC QVGFTCHAGRGPLEGPSGSVTC*LPVGQECQDQP VPCCGATGWWAGSQASPLQLMTLFGLSWGEGGV FPLTSPSCPMGKPWSHPFGANKTAPGL*QKEL* SSGG*RPAPPAGKSSPPQLQLIHRTTAGPDSPTC HMF*VSATRLPGGWVLPLAWTKGK*GPRSLPKL WQPSQATPMEAIKLFP

Frame 1

LWRRPCSPARCGLPPGSPAAVAAAYAAASAFLP RVGPGATSPLPPSPLWSFRKS*IM*RWDSWYLC AICC*PSASFWYWDFCIILRGSYTYSSGRRTPVS IVTLAHPRRSLLQIQWFFPLTPLDKH*AQSMIG WASS*IWTLNSHRGLSARWFLALTAYWNHLESL KNY*CLHPTL*RF*SH*ARTQPLH*DF*SCPGLL N*PPSTQTFQRELASLAMLQP**RPSSPGSPSQ HPCSWMTPFASGLESGSSCRLLGSKVKTI*SKP SCQSPKSTA*PLPWTASAAAAASSWAMEAFLPTS LWGHELTTMTLW*D*IQHQ*KALRRTWAAKRHC ASPTPRAPCSGLSSTSAILSLSSPASSGRTLSG *NTSSTRRE*VHRMASGNLWPLECPRSPLRFESS THISSRRPPSPSLACPSTMASWAGGTSLPLAVW Q*PWHYTAVTRWQSQDLAMT*AHPTHPCTTMRP FAWQPSKSPGRTISSERKSFCGSW*KLASSLI*A VASEWAQHMAIEAQAPPGAAASTTYTGVFRPRE GRCQGPQGQQGLGGAARAVPAQQPVSETSTQPH SAWVLDARGPAGSWLCPAGPACRWWDTGQQGCCR NHFSNQCLVYYHFVNLGRGEGRDNLFLNKVGDV KLGSLAMQEEAH*RAHQAVLPVSSLWGRSARTS LYLAVGLQDGGQDLKPAPSSS*HCLAFLGEKAGY SHSPALAVPWGNPGAIPSEPTRPPQGYSRKNFK AQEGDAQLRLLGRAPLHSCS*SIGLPQARTHQL ATCSRFQQQDCQVVGFCL*PGPKGSEAQGAYPSC GSRPRPPPWKQ*SSSL

Frame 2

YGGAHAAQRVVGSRRGPPRLSPPPTPLPPPSCP ASGRAPPPPCLPLRCGHLGNRKSCEDGTLGICA QSAASPLPLSGTGIFVLFCVEATLTPVGGGLQ* V*SL*LTPGEACCRFSGSFL*LRWTNTRLRV*SV GLPPESGL*TLTEDSLHGGFWP*LHIGITWRA* KITDACIPPSRDFEVTERGHSLCIRIFKAAQAC *ISHQVRKLFRGSLQAWLCFSLDDGHLPPVLQAS THVPG*LLSQVG*NPGVRAAFWDQRSRQSDQSH LVSHQRVPPDPCLGQPPLPPLHHRGQWRRSCQQ VSGVTN*RL*HCGETEFSTSERL*EGRGQQNDTA HHLPRGRHAAA*AVRARFSLCPRRLQVAGL*VV EIHRLQGESECIGWLLEICGHSSAQGAP*DSNP QPIFHPGGRLHPHWPALQQWPHGPGEHPYPWQCG SDHGTTRL*RGGSRRIWL*HEHTQRTPALL*DR SHGSHQRVLDAQYPARERVSAEAGESSRHH*SK QWHLSGPSTWP*RPRHHQEQQPAPPTQESSDPEK DGAKGPRGSKALVEQPELCLLSSQSQRPALSLI QHGSLMPEGQQAPGCAQQAQHAGGGTLGSKAAA GITSPISVWCIIIL*IWVGGRVGIIYF*IRLEMS SWVHLPCRKRPTRGPIRQCYLLAPCGAGVPGPA CTLLWGYRMVGRISSQPPPAHDTVWPFLGRRRG IPTHQP*LSHGETLEPSLRSQQDRPRAIAERTLK LRRVTPSSACWEELPSTAAADP*DYRRPGLTNL PHVLGFSNKTARWLGSAFSLDQREVRPKELTQA VAAVPGHPHGSNKALP*

Sequence NM_174968

ACTATGGCGGCGCCCATGCAGCCCAGCGCGTTGTGGGCTCCCGCCGGGGTCCCCCGCGGCTGTCGCCGCCGCCTACGCCGCTGCCTCCGCCTTCCTGCCC CGCGTCGGGCCGGGCGCCACCTCCCCCCTGCCTCCCTCTCCGCTGTGGTCATTTAGGAAATCGTAAATCATGTGAAGATGGGACTCTTGGTATTTGTGCG CAATCTGCTGCTAGCCCTCTGCCTCTTTCTGGTACTGGGATTTTTGTATTATTCTGCGTGGAAGCTACACTTACTCCAGTGGGAGGAGGACTCCAATTCA GTGGTTCTTTCCTTTGACTCCGCTGGACAAACACTAGGCTCAGAGTATGATCGGTTGGGCTTCCTCCTGAATCTGGACTCTAAACTCTCACCGAGGACTC TCTGCACGGTGGTTTTTGGCCTTGACTGCATATTGGAATCACCTGGAGAGCCTAAAAAATTACTGATGCCTGCATCCCACCCTCTAGAGATTTTGAAGTC ACTGAGCGAGGACACAGCCTTTGCATTAGGATTTTTAAAGCTGCCCAGGCCTGCTGAATTAGCCACCAAGTACGCAAACTTTTCAGAGGGAGCTTGCAAG CCTGGCTATGCTTCAGCCTTGATGACGGCCATCTTCCCCCGGTTCTCCAAGCCAGCACCCATGTTCCTGGATGACTCCTTTCGCAAGTGGGCTAGAATCC GGGAGTTCGTGCCGCCTTTTGGGATCAAAGGTCAAGACAATCTGATCAAAGCCATCTTGTCAGTCACCAAAGAGTACCGCCTGACCCCTGCCTTGGACAG CCTCCGCTGCCGCCGCTGCATCATCGTGGGCAATGGAGGCGTTCTTGCCAACAAGTCTCTGGGGTCACGAATTGACGACTATGACATTGTGGTGAGACTG AATTCAGCACCAGTGAAAGGCTTTGAGAAGGACGTGGGCAGCAAAACGACACTGCGCATCACCTACCCCGAGGGCGCCATGCAGCGGCCTGAGCAGTACG AGCGCGATTCTCTCTTTGTCCTCGCCGGCTTCAAGTGGCAGGACTTTAAGTGGTTGAAATACATCGTCTACAAGGAGAGAGTGAGTGCATCGGATGGCTT CTGGAAATCTGTGGCCACTCGAGTGCCCAAGGAGCCCCCTGAGATTCGAATCCTCAACCCATATTTCATCCAGGAGGCCGCCTTCACCCTCATTGGCCTG CCCTTCAACAATGGCCTCATGGGCCGGGGGAACATCCCTACCCTTGGCAGTGTGGCAGTGACCATGGCACTACACGGCTGTGACGAGGTGGCAGTCGCAG GATTTGGCTATGACATGAGCACACCCAACGCACCCCTGCACTACTATGAGACCGTTCGCATGGCAGCCATCAAAGAGTCCTGGACGCACAATATCCAGCG AGAGAAAGAGTTTCTGCGGAAGCTGGTGAAAGCTCGCGTCATCACTGATCTAAGCAGTGGCATCTGAGTGGGCCCAGCACATGGCCATAGAGGCCCAGGC ACCACCAGGAGCAGCAGCCAGCACCACCTACACAGGAGTCTTCAGACCCAGAGAAGGACGGTGCCAAGGGCCCCAGGGGCAGCAAGGCCTTGGTGGAGCA GCCAGAGCTGTGCCTGCTCAGCAGCCAGTCTCAGAGACCAGCACTCAGCCTCATTCAGCATGGGTCCTTGATGCCAGAGGGCCAGCAGGCTCCTGGCTGT GCCCAGCAGGCCCAGCATGCAGGTGGTGGGACACTGGGCAGCAAGGCTGCTGCCGGAATCACTTCTCCAATCAGTGTTTGGTGTATTATCATTTTGTGAA TTTGGGTAGGGGGGAGGGTAGGGATAATTTATTTTTAAATAAGGTTGGAGATGTCAAGTTGGGTTCACTTGCCATGCAGGAAGAGGCCCACTAGAGGGCC CATCAGGCAGTGTTACCTGTTAGCTCCCTGTGGGGCAGGAGTGCCAGGACCAGCCTGTACCTTGCTGTGGGGCTACAGGATGGTGGGCAGGATCTCAAGC CAGCCCCCTCCAGCTCATGACACTGTTTGGCCTTTCTTGGGGAGAAGGCGGGGTATTCCCACTCACCAGCCCTAGCTGTCCCATGGGGAAACCCTGGAGC CATCCCTTCGGAGCCAACAAGACCGCCCCAGGGCTATAGCAGAAAGAACTTTAAAGCTCAGGAGGGTGACGCCCAGCTCCGCCTGCTGGGAAGAGCTCCC CTCCACAGCTGCAGCTGATCCATAGGACTACCGCAGGCCCGGACTCACCAACTTGCCACATGTTCTAGGTTTCAGCAACAAGACTGCCAGGTGGTTGGGT TCTGCCTTTAGCCTGGACCAAAGGGAAGTGAGGCCCAAGGAGCTTACCCAAGCTGTGGCAGCCGTCCCAGGCCACCCCCATGGAAGCAATAAAGCTCTTC CCTGA

refseq_ST3GAL3.F5 (forward primer)
refseq_ST3GAL3.R5 (reverse primer)
Single exon skipping

Frame 0

TMAAPMQPSALWAPAGVPRGCRRRLRRCLRLPA PRRAGRHLPPASLSAVVI*EIVNHVKMGLLVFVR NLLLALCLFLVLGFLYYSAWKLHLLQWEEDSNS VVLSFDSAGQTLGSEYDRLGFLLNLDSKLSPRT LCTVVFGLDCILESPGEPKKLLMPASHPLEILKS LSEDTAFALGFLKLPRPAELATKYANFSEGACK PGYASALMTAIFPRFSKPAPMFLDDSFRKWARI REFVPPFGIKGQDNLIKAILSVTKEYRLTPALDS LRCRRCIIVGNGGVLANKSLGSRIDDYDIVVRL NSAPVKGFEKDVGSKTTLRITYPEGAMQRPEQY ERDSLFVLAGFKWQDFKWLKYIVYKERVSASDGF WKSVATRVPKEPPEIRILNPYFIQEAAFTLIGL PFNNGLMGRGNIPTLGSVAVTMALHGCDEVAVA GFGYDMSTPNAPLHYYETVRMAAIKESWTHNIQR EKEFLRKLVKARVITDLSSGI*VGPAHGHRGPG TTRSSSQHHLHRSLQTQRRTVPRAPGAARPWWS SQSCACSAASLRDQHSASFSMGP*CQRASRLLAV PSRPSMQVVGHWAARLLPESLLQSVFGVLSFCE FG*GGG*G*FIFK*GWRCQVGFTCHAGRGPLEG PSGSVTC*LPVGQECQDQPVPCCGATGWWAGSQA SPLQLMTLFGLSWGEGGVFPLTSPSCPMGKPWS HPFGANKTAPGL*QKEL*SSGG*RPAPPAGKSS PPQLQLIHRTTAGPDSPTCHMF*VSATRLPGGWV LPLAWTKGK*GPRSLPKLWQPSQATPMEAIKLF P

Frame 1

LWRRPCSPARCGLPPGSPAAVAAAYAAASAFLP RVGPGATSPLPPSPLWSFRKS*IM*RWDSWYLC AICC*PSASFWYWDFCIILRGSYTYSSGRRTPIQ WFFPLTPLDKH*AQSMIGWASS*IWTLNSHRGL SARWFLALTAYWNHLESLKNY*CLHPTL*RF*S H*ARTQPLH*DF*SCPGLLN*PPSTQTFQRELAS LAMLQP**RPSSPGSPSQHPCSWMTPFASGLES GSSCRLLGSKVKTI*SKPSCQSPKSTA*PLPWT ASAAAAASSWAMEAFLPTSLWGHELTTMTLW*D* IQHQ*KALRRTWAAKRHCASPTPRAPCSGLSST SAILSLSSPASSGRTLSG*NTSSTRRE*VHRMA SGNLWPLECPRSPLRFESSTHISSRRPPSPSLAC PSTMASWAGGTSLPLAVWQ*PWHYTAVTRWQSQ DLAMT*AHPTHPCTTMRPFAWQPSKSPGRTISS ERKSFCGSW*KLASSLI*AVASEWAQHMAIEAQA PPGAAASTTYTGVFRPREGRCQGPQGQQGLGGA ARAVPAQQPVSETSTQPHSAWVLDARGPAGSWL CPAGPACRWWDTGQQGCCRNHFSNQCLVYYHFVN LGRGEGRDNLFLNKVGDVKLGSLAMQEEAH*RA HQAVLPVSSLWGRSARTSLYLAVGLQDGGQDLK PAPSSS*HCLAFLGEKAGYSHSPALAVPWGNPGA IPSEPTRPPQGYSRKNFKAQEGDAQLRLLGRAP LHSCS*SIGLPQARTHQLATCSRFQQQDCQVVG FCL*PGPKGSEAQGAYPSCGSRPRPPPWKQ*SSS L

Frame 2

YGGAHAAQRVVGSRRGPPRLSPPPTPLPPPSCP ASGRAPPPPCLPLRCGHLGNRKSCEDGTLGICA QSAASPLPLSGTGIFVLFCVEATLTPVGGGLQF SGSFL*LRWTNTRLRV*SVGLPPESGL*TLTEDS LHGGFWP*LHIGITWRA*KITDACIPPSRDFEV TERGHSLCIRIFKAAQAC*ISHQVRKLFRGSLQ AWLCFSLDDGHLPPVLQASTHVPG*LLSQVG*NP GVRAAFWDQRSRQSDQSHLVSHQRVPPDPCLGQ PPLPPLHHRGQWRRSCQQVSGVTN*RL*HCGET EFSTSERL*EGRGQQNDTAHHLPRGRHAAA*AVR ARFSLCPRRLQVAGL*VVEIHRLQGESECIGWL LEICGHSSAQGAP*DSNPQPIFHPGGRLHPHWP ALQQWPHGPGEHPYPWQCGSDHGTTRL*RGGSRR IWL*HEHTQRTPALL*DRSHGSHQRVLDAQYPA RERVSAEAGESSRHH*SKQWHLSGPSTWP*RPR HHQEQQPAPPTQESSDPEKDGAKGPRGSKALVEQ PELCLLSSQSQRPALSLIQHGSLMPEGQQAPGC AQQAQHAGGGTLGSKAAAGITSPISVWCIIIL* IWVGGRVGIIYF*IRLEMSSWVHLPCRKRPTRGP IRQCYLLAPCGAGVPGPACTLLWGYRMVGRISS QPPPAHDTVWPFLGRRRGIPTHQP*LSHGETLE PSLRSQQDRPRAIAERTLKLRRVTPSSACWEELP STAAADP*DYRRPGLTNLPHVLGFSNKTARWLG SAFSLDQREVRPKELTQAVAAVPGHPHGSNKAL P*