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Sequence NM_016543
AACCCTGAGGAACAGACGTTCCCTCGCGGCCCTGGCACCTCCAACCCCAGATATGCTGCTGCTGCTGCTGCTGCCCCTGCTCTGGGGGAGGGAGAGGGTGGAAGGACAGAAGAGTAACCGGAAGGATTACTCGCTGACGATGCAGAGTTCCGTGACCGTGCAAGAGGGCATGTGTGTCCATGTGCGCTGCTCCTTCTCCT
ACCCAGTGGACAGCCAGACTGACTCTGACCCAGTTCATGGCTACTGGTTCCGGGCAGGGAATGATATAAGCTGGAAGGCTCCAGTGGCCACAAACAACCC
AGCTTGGGCAGTGCAGGAGGAAACTCGGGACCGATTCCACCTCCTTGGGGACCCACAGACCAAAAATTGCACCCTGAGCATCAGAGATGCCAGAATGAGT
GATGCGGGGAGATACTTCTTTCGTATGGAGAAAGGAAATATAAAATGGAATTATAAATATGACCAGCTCTCTGTGAACGTGACAGACCCTCCTCAGAACT
TGACTGTGACTGTCTTCCAAGGAGAAGGCACAGCATCCACAGCTCTGGGGAACAGCTCATCTCTTTCAGTCCTAGAGGGCCAGTCTCTGCGCTTGGTCTG
TGCTGTTGACAGCAATCCCCCTGCCAGGCTGAGCTGGACCTGGAGGAGTCTGACCCTGTACCCCTCACAGCCCTCAAACCCTCTGGTACTGGAGCTGCAA
GTGCACCTGGGGGATGAAGGGGAATTCACCTGTCGAGCTCAGAACTCTCTGGGTTCCCAGCACGTTTCCCTGAACCTCTCCCTGCAACAGGAGTACACAG
GCAAAATGAGGCCTGTATCAGGAGTGTTGCTGGGGGCGGTCGGGGGAGCTGGAGCCACAGCCCTGGTCTTCCTCTCCTTCTGTGTCATCTTCATTGTAGT
GAGGTCCTGCAGGAAGAAATCGGCAAGGCCAGCAGCGGACGTGGGAGACATAGGCATGAAGGATGCAAACACCATCAGGGGCTCAGCCTCTCAGGGTAAC
CTGACTGAGTCCTGGGCAGATGATAACCCCCGACACCATGGCCTGGCTGCCCACTCCTCAGGGGAGGAAAGAGAGATCCAGTATGCACCCCTCAGCTTTC
ATAAGGGGGAGCCTCAGGACCTATCAGGACAAGAAGCCACCAACAATGAGTACTCAGAGATCAAGATCCCCAAGTAAGAAAATGCAGAGGCTCGGGCTTG
TTTGAGGGTTCACGACCCCTCCAGCAAAGGAGTCTGAGGCTGATTCCAGTAGAATTAGCAGCCCTCAATGCTGTGCAACAAGACATCAGAACTTATTCCT
CTTGTCTAACTGAAAATGCATGCCTGATGACCAAACTCTCCCTTTCCCCATCCAATCGGTCCACACTCCCCGCCCTGGCCTCTGGTACCCACCATTCTCC
TCTGTACTTCTCTAAGGATGACTACTTTAGATTCCGAATATAGTGAGATTGTAACGTG
- refseq_SIGLEC7.F1 (forward primer)
- refseq_SIGLEC7.R1 (reverse primer)
- Single exon skipping
Frame 0
NPEEQTFPRGPGTSNPRYAAAAAAAPALGEGEGGRTEE*PEGLLADDAEFRDRARGHVCPCALLLLL
PSGQPD*L*PSSWLLVPGRE*YKLEGSSGHKQP
SLGSAGGNSGPIPPPWGPTDQKLHPEHQRCQNE
*CGEILLSYGERKYKMEL*I*PALCERDRPSSEL
DCDCLPRRRHSIHSSGEQLISFSPRGPVSALGL
CC*QQSPCQAELDLEESDPVPLTALKPSGTGAA
SAPGG*RGIHLSSSELSGFPARFPEPLPATGVHR
QNEACIRSVAGGGRGSWSHSPGLPLLLCHLHCS
EVLQEEIGKASSGRGRHRHEGCKHHQGLSLSG*
PD*VLGR**PPTPWPGCPLLRGGKRDPVCTPQLS
*GGASGPIRTRSHQQ*VLRDQDPQVRKCRGSGL
FEGSRPLQQRSLRLIPVELAALNAVQQDIRTYS
SCLTENACLMTKLSLSPSNRSTLPALASGTHHSP
LYFSKDDYFRFRI**DCNV
Frame 1
TLRNRRSLAALAPPTPDMLLLLLLPLLWGRERVEGQKSNRKDYSLTMQSSVTVQEGMCVHVRCSFS
YPVDSQTDSDPVHGYWFRAGNDISWKAPVATNNP
AWAVQEETRDRFHLLGDPQTKNCTLSIRDARMS
DAGRYFFRMEKGNIKWNYKYDQLSVNVTDPPQN
LTVTVFQGEGTASTALGNSSSLSVLEGQSLRLVC
AVDSNPPARLSWTWRSLTLYPSQPSNPLVLELQ
VHLGDEGEFTCRAQNSLGSQHVSLNLSLQQEYT
GKMRPVSGVLLGAVGGAGATALVFLSFCVIFIVV
RSCRKKSARPAADVGDIGMKDANTIRGSASQGN
LTESWADDNPRHHGLAAHSSGEEREIQYAPLSF
HKGEPQDLSGQEATNNEYSEIKIPK*ENAEARAC
LRVHDPSSKGV*G*FQ*N*QPSMLCNKTSELIP
LV*LKMHA**PNSPFPHPIGPHSPPWPLVPTIL
LCTSLRMTTLDSEYSEIVT
Frame 2
P*GTDVPSRPWHLQPQICCCCCCCPCSGGGRGWKDRRVTGRITR*RCRVP*PCKRACVSMCAAPSP
TQWTARLTLTQFMATGSGQGMI*AGRLQWPQTT
QLGQCRRKLGTDSTSLGTHRPKIAP*ASEMPE*V
MRGDTSFVWRKEI*NGIINMTSSL*T*QTLLRT
*L*LSSKEKAQHPQLWGTAHLFQS*RASLCAWS
VLLTAIPLPG*AGPGGV*PCTPHSPQTLWYWSCK
CTWGMKGNSPVELRTLWVPSTFP*TSPCNRSTQ
AK*GLYQECCWGRSGELEPQPWSSSPSVSSSL*
*GPAGRNRQGQQRTWET*A*RMQTPSGAQPLRVT
*LSPGQMITPDTMAWLPTPQGRKERSSMHPSAF
IRGSLRTYQDKKPPTMSTQRSRSPSKKMQRLGL
V*GFTTPPAKESEADSSRISSPQCCATRHQNLFL
LSN*KCMPDDQTLPFPIQSVHTPRPGLWYPPFS
SVLL*G*LL*IPNIVRL*R
Sequence NM_014385
AACCCTGAGGAACAGACGTTCCCTCGCGGCCCTGGCACCTCCAACCCCAGATATGCTGCTGCTGCTGCTGCTGCCCCTGCTCTGGGGGAGGGAGAGGGTGGAAGGACAGAAGAGTAACCGGAAGGATTACTCGCTGACGATGCAGAGTTCCGTGACCGTGCAAGAGGGCATGTGTGTCCATGTGCGCTGCTCCTTCTCCT
ACCCAGTGGACAGCCAGACTGACTCTGACCCAGTTCATGGCTACTGGTTCCGGGCAGGGAATGATATAAGCTGGAAGGCTCCAGTGGCCACAAACAACCC
AGCTTGGGCAGTGCAGGAGGAAACTCGGGACCGATTCCACCTCCTTGGGGACCCACAGACCAAAAATTGCACCCTGAGCATCAGAGATGCCAGAATGAGT
GATGCGGGGAGATACTTCTTTCGTATGGAGAAAGGAAATATAAAATGGAATTATAAATATGACCAGCTCTCTGTGAACGTGACAGCCTTGACCCACAGGC
CCAACATCCTTATCCCCGGTACCCTGGAGTCTGGCTGCTTCCAGAATCTGACCTGCTCTGTGCCCTGGGCCTGTGAGCAGGGGACGCCCCCTATGATCTC
CTGGATGGGGACCTCTGTGTCCCCCCTGCACCCCTCCACCACCCGCTCCTCAGTGCTCACCCTCATCCCACAGCCCCAGCACCACGGCACCAGCCTCACC
TGTCAGGTGACCTTGCCTGGGGCCGGCGTGACCACGAACAGGACCATCCAACTCAATGTGTCCTACCCTCCTCAGAACTTGACTGTGACTGTCTTCCAAG
GAGAAGGCACAGCATCCACAGCTCTGGGGAACAGCTCATCTCTTTCAGTCCTAGAGGGCCAGTCTCTGCGCTTGGTCTGTGCTGTTGACAGCAATCCCCC
TGCCAGGCTGAGCTGGACCTGGAGGAGTCTGACCCTGTACCCCTCACAGCCCTCAAACCCTCTGGTACTGGAGCTGCAAGTGCACCTGGGGGATGAAGGG
GAATTCACCTGTCGAGCTCAGAACTCTCTGGGTTCCCAGCACGTTTCCCTGAACCTCTCCCTGCAACAGGAGTACACAGGCAAAATGAGGCCTGTATCAG
GAGTGTTGCTGGGGGCGGTCGGGGGAGCTGGAGCCACAGCCCTGGTCTTCCTCTCCTTCTGTGTCATCTTCATTGTAGTGAGGTCCTGCAGGAAGAAATC
GGCAAGGCCAGCAGCGGACGTGGGAGACATAGGCATGAAGGATGCAAACACCATCAGGGGCTCAGCCTCTCAGGGTAACCTGACTGAGTCCTGGGCAGAT
GATAACCCCCGACACCATGGCCTGGCTGCCCACTCCTCAGGGGAGGAAAGAGAGATCCAGTATGCACCCCTCAGCTTTCATAAGGGGGAGCCTCAGGACC
TATCAGGACAAGAAGCCACCAACAATGAGTACTCAGAGATCAAGATCCCCAAGTAAGAAAATGCAGAGGCTCGGGCTTGTTTGAGGGTTCACGACCCCTC
CAGCAAAGGAGTCTGAGGCTGATTCCAGTAGAATTAGCAGCCCTCAATGCTGTGCAACAAGACATCAGAACTTATTCCTCTTGTCTAACTGAAAATGCAT
GCCTGATGACCAAACTCTCCCTTTCCCCATCCAATCGGTCCACACTCCCCGCCCTGGCCTCTGGTACCCACCATTCTCCTCTGTACTTCTCTAAGGATGA
CTACTTTAGATTCCGAATATAGTGAGATTGTAACGTG
- refseq_SIGLEC7.F1 (forward primer)
- refseq_SIGLEC7.R1 (reverse primer)
- Single exon skipping
Frame 0
NPEEQTFPRGPGTSNPRYAAAAAAAPALGEGEGGRTEE*PEGLLADDAEFRDRARGHVCPCALLLLL
PSGQPD*L*PSSWLLVPGRE*YKLEGSSGHKQP
SLGSAGGNSGPIPPPWGPTDQKLHPEHQRCQNE
*CGEILLSYGERKYKMEL*I*PALCERDSLDPQA
QHPYPRYPGVWLLPESDLLCALGL*AGDAPYDL
LDGDLCVPPAPLHHPLLSAHPHPTAPAPRHQPH
LSGDLAWGRRDHEQDHPTQCVLPSSELDCDCLPR
RRHSIHSSGEQLISFSPRGPVSALGLCC*QQSP
CQAELDLEESDPVPLTALKPSGTGAASAPGG*R
GIHLSSSELSGFPARFPEPLPATGVHRQNEACIR
SVAGGGRGSWSHSPGLPLLLCHLHCSEVLQEEI
GKASSGRGRHRHEGCKHHQGLSLSG*PD*VLGR
**PPTPWPGCPLLRGGKRDPVCTPQLS*GGASGP
IRTRSHQQ*VLRDQDPQVRKCRGSGLFEGSRPL
QQRSLRLIPVELAALNAVQQDIRTYSSCLTENA
CLMTKLSLSPSNRSTLPALASGTHHSPLYFSKDD
YFRFRI**DCNV
Frame 1
TLRNRRSLAALAPPTPDMLLLLLLPLLWGRERVEGQKSNRKDYSLTMQSSVTVQEGMCVHVRCSFS
YPVDSQTDSDPVHGYWFRAGNDISWKAPVATNNP
AWAVQEETRDRFHLLGDPQTKNCTLSIRDARMS
DAGRYFFRMEKGNIKWNYKYDQLSVNVTALTHR
PNILIPGTLESGCFQNLTCSVPWACEQGTPPMIS
WMGTSVSPLHPSTTRSSVLTLIPQPQHHGTSLT
CQVTLPGAGVTTNRTIQLNVSYPPQNLTVTVFQ
GEGTASTALGNSSSLSVLEGQSLRLVCAVDSNPP
ARLSWTWRSLTLYPSQPSNPLVLELQVHLGDEG
EFTCRAQNSLGSQHVSLNLSLQQEYTGKMRPVS
GVLLGAVGGAGATALVFLSFCVIFIVVRSCRKKS
ARPAADVGDIGMKDANTIRGSASQGNLTESWAD
DNPRHHGLAAHSSGEEREIQYAPLSFHKGEPQD
LSGQEATNNEYSEIKIPK*ENAEARACLRVHDPS
SKGV*G*FQ*N*QPSMLCNKTSELIPLV*LKMH
A**PNSPFPHPIGPHSPPWPLVPTILLCTSLRM
TTLDSEYSEIVT
Frame 2
P*GTDVPSRPWHLQPQICCCCCCCPCSGGGRGWKDRRVTGRITR*RCRVP*PCKRACVSMCAAPSP
TQWTARLTLTQFMATGSGQGMI*AGRLQWPQTT
QLGQCRRKLGTDSTSLGTHRPKIAP*ASEMPE*V
MRGDTSFVWRKEI*NGIINMTSSL*T*QP*PTG
PTSLSPVPWSLAASRI*PALCPGPVSRGRPL*S
PGWGPLCPPCTPPPPAPQCSPSSHSPSTTAPASP
VR*PCLGPA*PRTGPSNSMCPTLLRT*L*LSSK
EKAQHPQLWGTAHLFQS*RASLCAWSVLLTAIP
LPG*AGPGGV*PCTPHSPQTLWYWSCKCTWGMKG
NSPVELRTLWVPSTFP*TSPCNRSTQAK*GLYQ
ECCWGRSGELEPQPWSSSPSVSSSL**GPAGRN
RQGQQRTWET*A*RMQTPSGAQPLRVT*LSPGQM
ITPDTMAWLPTPQGRKERSSMHPSAFIRGSLRT
YQDKKPPTMSTQRSRSPSKKMQRLGLV*GFTTP
PAKESEADSSRISSPQCCATRHQNLFLLSN*KCM
PDDQTLPFPIQSVHTPRPGLWYPPFSSVLL*G*
LL*IPNIVRL*R
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