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Sequence NM_003345
GGGTCCTCGGAGCTGCTCTGGCTGCGCGCGGAGCGGGCTCCGGAGGGAAGTCCCGAGACAAAGGGAAGCGCCGCCGCCGCCGCCCCGCTCGGTCCTCCACCTGTCCGCTACGCTCGCCGGGGCTGCGGCCGCCCGAGGGACTTTGAACATGTCGGGGATCGCCCTCAGCAGACTCGCCCAGGAGAGGAAAGCATGGAGGA
AAGACCACCCATTTGGTTTCGTGGCTGTCCCAACAAAAAATCCCGATGGCACGATGAACCTCATGAACTGGGAGTGCGCCATTCCAGGAAAGAAAGGGAC
TCCGTGGGAAGGAGGCTTGTTTAAACTACGGATGCTTTTCAAAGATGATTATCCATCTTCGCCACCAAAATGTAAATTCGAACCACCATTATTTCACCCG
AATGTGTACCCTTCGGGGACAGTGTGCCTGTCCATCTTAGAGGAGGACAAGGACTGGAGGCCAGCCATCACAATCAAACAGATCCTATTAGGAATACAGG
AACTTCTAAATGAACCAAATATCCAAGACCCAGCTCAAGCAGAGGCCTACACGATTTACTGCCAAAACAGAGTGGAGTACGAGAAAAGGGTCCGAGCACA
AGCCAAGAAGTTTGCGCCCTCATAAGCAGCGACCTTGTGGCATCGTCAAAAGGAAGGGATTGGTTTGGCAAGAACTTGTTTACAACATTTTTGCAAATCT
AAAGTTGCTCCATACAATGACTAGTCACCTGGGGGGGTTGGGCGGGCGCCATCTTCCATTGCCGCCGCGGGTGTGCGGTCTCGATTCGCTGAATTGCCCG
TTTCCATACAGGGTCTCTTCCTTCGGTCTTTTGTATTTTTGATTGTTATGTAAAACTCGCTTTTATTTTAATATTGATGTCAGTATTTCAACTGCTGTAA
AATTATAAACTTTTATACTTGGGTAAGTCCCCCAGGGGCGAGTTCCTCGCTCTGGGATGCAGGCATGCTTCTCACCGTGCAGAGCTGCACTTGGCCTCAG
CTGGCTGTATGGAAATGCACCCTCCCTCCTGCCGCTCCTCTCTAGAACCTTCTAGAACCTGGGCTGTGCTGCTTTTGAGCCTCAGACCCCAGGTCAGCAT
CTCGGTTCTGCGCCACTTCCTTTGTGTTTATATGGCGTTTTGTCTGTGTTGCTGTTTAGAGTAAATAAACTGTTTATATAAAGGTTTTGGTTGCATTATT
ATCATTGAAAGTGAGAGGAGG
- refseq_UBE2I.F1 (forward primer)
- refseq_UBE2I.R1 (reverse primer)
- Single exon skipping
Frame 0
GSSELLWLRAERAPEGSPETKGSAAAAAPLGPPPVRYARRGCGRPRDFEHVGDRPQQTRPGEESMEE
RPPIWFRGCPNKKSRWHDEPHELGVRHSRKERD
SVGRRLV*TTDAFQR*LSIFATKM*IRTTIISP
ECVPFGDSVPVHLRGGQGLEASHHNQTDPIRNTG
TSK*TKYPRPSSSRGLHDLLPKQSGVREKGPST
SQEVCALISSDLVASSKGRDWFGKNLFTTFLQI
*SCSIQ*LVTWGGWAGAIFHCRRGCAVSIR*IAR
FHTGSLPSVFCIFDCYVKLAFILILMSVFQLL*
NYKLLYLGKSPRGEFLALGCRHASHRAELHLAS
AGCMEMHPPSCRSSLEPSRTWAVLLLSLRPQVSI
SVLRHFLCVYMAFCLCCCLE*INCLYKGFGCII
IIESERR
Frame 1
GPRSCSGCARSGLRREVPRQREAPPPPPRSVLHLSATLAGAAAARGTLNMSGIALSRLAQERKAWR
KDHPFGFVAVPTKNPDGTMNLMNWECAIPGKKGT
PWEGGLFKLRMLFKDDYPSSPPKCKFEPPLFHP
NVYPSGTVCLSILEEDKDWRPAITIKQILLGIQ
ELLNEPNIQDPAQAEAYTIYCQNRVEYEKRVRAQ
AKKFAPS*AATLWHRQKEGIGLARTCLQHFCKS
KVAPYND*SPGGVGRAPSSIAAAGVRSRFAELP
VSIQGLFLRSFVFLIVM*NSLLF*Y*CQYFNCCK
IINFYTWVSPPGASSSLWDAGMLLTVQSCTWPQ
LAVWKCTLPPAAPL*NLLEPGLCCF*ASDPRSA
SRFCATSFVFIWRFVCVAV*SK*TVYIKVLVALL
SLKVRG
Frame 2
VLGAALAARGAGSGGKSRDKGKRRRRRPARSSTCPLRSPGLRPPEGL*TCRGSPSADSPRRGKHGG
KTTHLVSWLSQQKIPMAR*TS*TGSAPFQERKG
LRGKEACLNYGCFSKMIIHLRHQNVNSNHHYFTR
MCTLRGQCACPS*RRTRTGGQPSQSNRSY*EYR
NF*MNQISKTQLKQRPTRFTAKTEWSTRKGSEH
KPRSLRPHKQRPCGIVKRKGLVWQELVYNIFANL
KLLHTMTSHLGGLGGRHLPLPPRVCGLDSLNCP
FPYRVSSFGLLYF*LLCKTRFYFNIDVSISTAV
KL*TFILG*VPQGRVPRSGMQACFSPCRAALGLS
WLYGNAPSLLPLLSRTF*NLGCAAFEPQTPGQH
LGSAPLPLCLYGVLSVLLFRVNKLFI*RFWLHY
YH*K*EE
Sequence NM_194259
GGGTCCTCGGAGCTGCTCTGGCTGCGCGCGGAGCGGGCTCCGGAGGGAAGTCCCGAGACAAAGGGAAGCGCCGCCGCCGCCGCCCCGCTCGGTCCTCCACCTGTCCGCTACGCTCGCCGGGGCTGCGGCCGCCCGAGGCTGCCCTGAGGATCTGTGTTTGGTGAAAAGGAGCCAAATTCACCTGCAGGGCAGGCGGCTCT
AGCAGCTTCAGAAGCCTGGTGCCCTGGCGACACTGGACCTGCCTTGGCTTCTTTGATCCCAACCCCACCCCCGATTTCTGCTCTGCTGACTGGGGAAGTC
ATCGTGCCACCCAGAACCTGAGTGCGGGCCTCTCAGAGCTCCTTCGTCCGTGGGTCTGCCGGGGACTGGGCCTTGTCTCCCTAACGAGTGCCAGGGACTT
TGAACATGTCGGGGATCGCCCTCAGCAGACTCGCCCAGGAGAGGAAAGCATGGAGGAAAGACCACCCATTTGGTTTCGTGGCTGTCCCAACAAAAAATCC
CGATGGCACGATGAACCTCATGAACTGGGAGTGCGCCATTCCAGGAAAGAAAGGGACTCCGTGGGAAGGAGGCTTGTTTAAACTACGGATGCTTTTCAAA
GATGATTATCCATCTTCGCCACCAAAATGTAAATTCGAACCACCATTATTTCACCCGAATGTGTACCCTTCGGGGACAGTGTGCCTGTCCATCTTAGAGG
AGGACAAGGACTGGAGGCCAGCCATCACAATCAAACAGATCCTATTAGGAATACAGGAACTTCTAAATGAACCAAATATCCAAGACCCAGCTCAAGCAGA
GGCCTACACGATTTACTGCCAAAACAGAGTGGAGTACGAGAAAAGGGTCCGAGCACAAGCCAAGAAGTTTGCGCCCTCATAAGCAGCGACCTTGTGGCAT
CGTCAAAAGGAAGGGATTGGTTTGGCAAGAACTTGTTTACAACATTTTTGCAAATCTAAAGTTGCTCCATACAATGACTAGTCACCTGGGGGGGTTGGGC
GGGCGCCATCTTCCATTGCCGCCGCGGGTGTGCGGTCTCGATTCGCTGAATTGCCCGTTTCCATACAGGGTCTCTTCCTTCGGTCTTTTGTATTTTTGAT
TGTTATGTAAAACTCGCTTTTATTTTAATATTGATGTCAGTATTTCAACTGCTGTAAAATTATAAACTTTTATACTTGGGTAAGTCCCCCAGGGGCGAGT
TCCTCGCTCTGGGATGCAGGCATGCTTCTCACCGTGCAGAGCTGCACTTGGCCTCAGCTGGCTGTATGGAAATGCACCCTCCCTCCTGCCGCTCCTCTCT
AGAACCTTCTAGAACCTGGGCTGTGCTGCTTTTGAGCCTCAGACCCCAGGTCAGCATCTCGGTTCTGCGCCACTTCCTTTGTGTTTATATGGCGTTTTGT
CTGTGTTGCTGTTTAGAGTAAATAAACTGTTTATATAAAGGTTTTGGTTGCATTATTATCATTGAAAGTGAGAGGAGG
- refseq_UBE2I.F1 (forward primer)
- refseq_UBE2I.R1 (reverse primer)
- Single exon skipping
Frame 0
GSSELLWLRAERAPEGSPETKGSAAAAAPLGPPPVRYARRGCGRPRLP*GSVFGEKEPNSPAGQAAL
AASEAWCPGDTGPALASLIPTPPPISALLTGEV
IVPPRT*VRASQSSFVRGSAGDWALSP*RVPGT
LNMSGIALSRLAQERKAWRKDHPFGFVAVPTKNP
DGTMNLMNWECAIPGKKGTPWEGGLFKLRMLFK
DDYPSSPPKCKFEPPLFHPNVYPSGTVCLSILE
EDKDWRPAITIKQILLGIQELLNEPNIQDPAQAE
AYTIYCQNRVEYEKRVRAQAKKFAPS*AATLWH
RQKEGIGLARTCLQHFCKSKVAPYND*SPGGVG
RAPSSIAAAGVRSRFAELPVSIQGLFLRSFVFLI
VM*NSLLF*Y*CQYFNCCKIINFYTWVSPPGAS
SSLWDAGMLLTVQSCTWPQLAVWKCTLPPAAPL
*NLLEPGLCCF*ASDPRSASRFCATSFVFIWRFV
CVAV*SK*TVYIKVLVALLSLKVRG
Frame 1
GPRSCSGCARSGLRREVPRQREAPPPPPRSVLHLSATLAGAAAARGCPEDLCLVKRSQIHLQGRRL
*QLQKPGALATLDLPWLL*SQPHPRFLLC*LGKS
SCHPEPECGPLRAPSSVGLPGTGPCLPNECQGL
*TCRGSPSADSPRRGKHGGKTTHLVSWLSQQKI
PMAR*TS*TGSAPFQERKGLRGKEACLNYGCFSK
MIIHLRHQNVNSNHHYFTRMCTLRGQCACPS*R
RTRTGGQPSQSNRSY*EYRNF*MNQISKTQLKQ
RPTRFTAKTEWSTRKGSEHKPRSLRPHKQRPCGI
VKRKGLVWQELVYNIFANLKLLHTMTSHLGGLG
GRHLPLPPRVCGLDSLNCPFPYRVSSFGLLYF*
LLCKTRFYFNIDVSISTAVKL*TFILG*VPQGRV
PRSGMQACFSPCRAALGLSWLYGNAPSLLPLLS
RTF*NLGCAAFEPQTPGQHLGSAPLPLCLYGVL
SVLLFRVNKLFI*RFWLHYYH*K*EE
Frame 2
VLGAALAARGAGSGGKSRDKGKRRRRRPARSSTCPLRSPGLRPPEAALRICVW*KGAKFTCRAGGS
SSFRSLVPWRHWTCLGFFDPNPTPDFCSADWGS
HRATQNLSAGLSELLRPWVCRGLGLVSLTSARDF
EHVGDRPQQTRPGEESMEERPPIWFRGCPNKKS
RWHDEPHELGVRHSRKERDSVGRRLV*TTDAFQ
R*LSIFATKM*IRTTIISPECVPFGDSVPVHLRG
GQGLEASHHNQTDPIRNTGTSK*TKYPRPSSSR
GLHDLLPKQSGVREKGPSTSQEVCALISSDLVA
SSKGRDWFGKNLFTTFLQI*SCSIQ*LVTWGGWA
GAIFHCRRGCAVSIR*IARFHTGSLPSVFCIFD
CYVKLAFILILMSVFQLL*NYKLLYLGKSPRGE
FLALGCRHASHRAELHLASAGCMEMHPPSCRSSL
EPSRTWAVLLLSLRPQVSISVLRHFLCVYMAFC
LCCCLE*INCLYKGFGCIIIIESERR
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