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Sequences

Sequence NM_182509

AGAGCAGCCTCGGAACCGAGACGATGCGTGCGCTCCGCGACCGAGCCGGGCTCCTCCTCTGCGTGCTGCTGCTGGCGGCGCTGCTGGAGGCGGCGCTAGG GCTCCCCGTGAAGAAGCCGCGGCTCCGCGGACCACGGCCTGGGAGCCTCACGAGGCTCGCAGAGGTCTCAGCCTCCCCAGATCCTAGGCCTCTGAAGGAA GAGGAGGAGGCACCACTGCTCCCCAGAACCCACCTGCAGGCAGAGCCACACCAACATGGATGCTGGACTGTCACTGAGCCAGCAGCCATGACCCCAGGCA ACGCCACCCCTCCCAGGACCCCAGAGGTTACTCCGTTGCGGCTGGAGCTGCAGAAGCTGCCGGGATTGGCCAACACAACCTTGAGTACCCCTAACCCTGA TACCCAGGCTTCAGCCTCCCCAGATCCTAGGCCTCTGAGGGAAGAGGAGGAGGCACGACTGCTCCCCAGAACCCACCTGCAGGCAGAGCTACACCAACAT GGATGTTGGACTGTCACTGAGCCAGCAGCCCTGACCCCAGGGAATGCCACGCCTCCCAGGACCCAGGAGGTTACTCCCTTGCTGCTGGAGCTGCAGAAGC TGCCAGAATTGGTCCACGCAACCTTGAGTACCCCTAACCCTGATAACCAGACTATGAGCCTCAGAAGGAGTGGAGTCCCTGGTCTCCCTGCAGTGGGAAC TGCAGCACTGGCAAGCAGCAGAGGACTCGGCCCTGTGGCTATGGCTGCACTGCCACCGAGACCCGTACCTGTGACCTGCCCTCCTGTCCTGGCACTGAGG ACAAGGACACCTTGGGCCTCCCCAGTGAGGAGTGGAAGCTCCTGGCCCGCAATGCTACGGACATGCATGATCAAGATGTGGACAGCTGTGAGAAGTGGCT GAACTGCAAGAGCGACTTCCTAATCAAGTATCTGAGCCAGATGCTGCGGGACCTGCCCAGCTGCCCGTGTGCCTACCCACTGGAGGCCATGGACAGCCCT GTGAGCCTACAGGACGAGCACCAGGGCCGCAGCTTCCGGTGGAGGGATGCCAGTGGCCCTCGCGAGCGCCTGGACATCTACCAGCCCACGGCGCGCTTCT GCCTGCGTTCCATGCTGTCTGGGGAGAGCAGCACACTGGCCGCCCAGCACTGCTGCTATGACGAGGACAGCCGGCTGCTGACCCGTGGCAAGGGCGCCGG CATGCCCAACCTCATCAGCACCGACTTCTCACCTAAGCTGCACTTCAAGTTCGACACGACGCCCTGGATCCTGTGCAAGGGGGACTGGAGCCGCCTCCAC GCTGTGCTCCCTCCCAACAACGGCCGAGCCTGCACCGACAACCCCCTGGAGGAGGAGTACCTAGCACAGTTGCAGGAGGCCAAGGAGTACTAGTGACGGG GTTGCTGAACAGACACTGCAGGGAGAGGGCAGGCGGCTGCTGCTGTTGCACGGGAGAACTTTCCTCACCCGCCCCTGCCCAGACAGGGTGAGGAAAGGGC TCCCCCAGTGAGGTTGGTCCGAGGCTGTGTGCCCTCTGCCAGCGACCCCGAAGCAGATATCTCAGTGGGGTTAGTGAGAAGGTTGAAGGGTATGTAGGGC CCAGGGTGGGTGTCCCTGGGAGCCCTGGAAATGTGCATATGTGCATGTGTCTGCCGGGGCCTCCCTCTGCTGCCTGCTGGGACCCTGGCCACTCATTTTT CTCCTCCTTGGGAGCTGGGCTCTTCTGCCCTGGCTCTGCACATAAGTGTTAGCCAGCAGCTCCAGAAAAATCCCGATTCCCGGGATCTGCCACGAGTCAC TCCTACTCCACCCTGATGGCCAGCAGAGGAAGGGCCACTCTTCTCATGGGCACAGCCATCCTTTGCCGGGGGGGGCATCCAGCCTGGGTGGCCACCCCTC CTTATCTCTGGGTGGTGCACATGCCCTTCTTTCCCCACTCCCTGCCACGAGCCACTGCACAGGAGGCTATCTGTAGCCCCAAGCTGCCTTTCTGTTGGAC ACCAACTTTAGTCTTGGGCTGCAAGCCAGCCCAGCTGAGGCGAAGTGGACTCCAGGCAGGGAATGGGTTGCCCAATTCTGGTCCCTTTCCTTTGCTCAGC CCCCTCTGTTCTGCTGATTGTAGGGATGTGCAGGGCTGGGAGTTGGCACTCCCCCCGAGTGGGGAGGTGACAGCTTGTCACAGTAGCCAGGCTTGGGTGG GTTCAGCACTAGCTCGGGACGGTGTGTCACACGTCTATAGTAAACCAGTTCTCTGGGAGGGGAAAAAAGCCCTGATTTATTGCATTTGGGCAGCTTCTGT GGTGTAAATTCTCCCAGCAGTGTCCCATGTCATGCTGCCAGCATCACTGAATGCACTGAACTCAGAGTTGGGAAGAGATGCACATAATCGCTCTCCCGGC ACACCTCATGCCTCTTCCCTGCCTCCCCATTCCCCTGGCTGCACTTCCTTGCCTTCTATGGGGTTGAAATATTGAAGTCTCAACTGTCTCTGTTCACAAG AGCCACCAAAAGTTAGGGGACTTCAGTCCTAGCCCCCAGATGGCCGCCCTGAAGCTCTCTGGGCTCCTCAGCAATAAAGCACTTTATTTTCAAA

refseq_THSD3.F3 (forward primer)
refseq_THSD3.R3 (reverse primer)
Single exon skipping

Frame 0

RAASEPRRCVRSATEPGSSSACCCWRRCWRRR* GSP*RSRGSADHGLGASRGSQRSQPPQILGL*RK RRRHHCSPEPTCRQSHTNMDAGLSLSQQP*PQA TPPLPGPQRLLRCGWSCRSCRDWPTQP*VPLTL IPRLQPPQILGL*GKRRRHDCSPEPTCRQSYTNM DVGLSLSQQP*PQGMPRLPGPRRLLPCCWSCRS CQNWSTQP*VPLTLITRL*ASEGVESLVSLQWE LQHWQAAEDSALWLWLHCHRDPYL*PALLSWH*G QGHLGPPQ*GVEAPGPQCYGHA*SRCGQL*EVA ELQERLPNQVSEPDAAGPAQLPVCLPTGGHGQP CEPTGRAPGPQLPVEGCQWPSRAPGHLPAHGALL PAFHAVWGEQHTGRPALLL*RGQPAADPWQGRR HAQPHQHRLLT*AALQVRHDALDPVQGGLEPPP RCAPSQQRPSLHRQPPGGGVPSTVAGGQGVLVTG LLNRHCRERAGGCCCCTGELSSPAPAQTG*GKG SPSEVGPRLCALCQRPRSRYLSGVSEKVEGYVG PRVGVPGSPGNVHMCMCLPGPPSAACWDPGHSFF SSLGAGLFCPGSAHKC*PAAPEKSRFPGSATSH SYSTLMASRGRATLLMGTAILCRGGHPAWVATP PYLWVVHMPFFPHSLPRATAQEAICSPKLPFCWT PTLVLGCKPAQLRRSGLQAGNGLPNSGPFPLLS PLCSADCRDVQGWELALPPSGEVTACHSSQAWV GSALARDGVSHVYSKPVLWEGKKALIYCIWAASV V*ILPAVSHVMLPASLNALNSELGRDAHNRSPG TPHASSLPPHSPGCTSLPSMGLKY*SLNCLCSQ EPPKVRGLQS*PPDGRPEALWAPQQ*STLFS

Frame 1

EQPRNRDDACAPRPSRAPPLRAAAGGAAGGGAR APREEAAAPRTTAWEPHEARRGLSLPRS*ASEG RGGGTTAPQNPPAGRATPTWMLDCH*ASSHDPRQ RHPSQDPRGYSVAAGAAEAAGIGQHNLEYP*P* YPGFSLPRS*ASEGRGGGTTAPQNPPAGRATPT WMLDCH*ASSPDPRECHASQDPGGYSLAAGAAEA ARIGPRNLEYP*P**PDYEPQKEWSPWSPCSGN CSTGKQQRTRPCGYGCTATETRTCDLPSCPGTE DKDTLGLPSEEWKLLARNATDMHDQDVDSCEKWL NCKSDFLIKYLSQMLRDLPSCPCAYPLEAMDSP VSLQDEHQGRSFRWRDASGPRERLDIYQPTARF CLRSMLSGESSTLAAQHCCYDEDSRLLTRGKGAG MPNLISTDFSPKLHFKFDTTPWILCKGDWSRLH AVLPPNNGRACTDNPLEEEYLAQLQEAKEY**R GC*TDTAGRGQAAAAVARENFPHPPLPRQGEERA PPVRLVRGCVPSASDPEADISVGLVRRLKGM*G PGWVSLGALEMCICACVCRGLPLLPAGTLATHF SPPWELGSSALALHISVSQQLQKNPDSRDLPRVT PTPP*WPAEEGPLFSWAQPSFAGGGIQPGWPPL LISGWCTCPSFPTPCHEPLHRRLSVAPSCLSVG HQL*SWAASQPS*GEVDSRQGMGCPILVPFLCSA PSVLLIVGMCRAGSWHSPRVGR*QLVTVARLGW VQH*LGTVCHTSIVNQFSGRGKKP*FIAFGQLL WCKFSQQCPMSCCQHH*MH*TQSWEEMHIIALPA HLMPLPCLPIPLAALPCLLWG*NIEVSTVSVHK SHQKLGDFSPSPQMAALKLSGLLSNKALYFQ

Frame 2

SSLGTETMRALRDRAGLLLCVLLLAALLEAALG LPVKKPRLRGPRPGSLTRLAEVSASPDPRPLKE EEEAPLLPRTHLQAEPHQHGCWTVTEPAAMTPG NATPPRTPEVTPLRLELQKLPGLANTTLSTPNPD TQASASPDPRPLREEEEARLLPRTHLQAELHQH GCWTVTEPAALTPGNATPPRTQEVTPLLLELQK LPELVHATLSTPNPDNQTMSLRRSGVPGLPAVGT AALASSRGLGPVAMAALPPRPVPVTCPPVLALR TRTPWASPVRSGSSWPAMLRTCMIKMWTAVRSG *TARATS*SSI*ARCCGTCPAARVPTHWRPWTAL *AYRTSTRAAASGGGMPVALASAWTSTSPRRAS ACVPCCLGRAAHWPPSTAAMTRTAGC*PVARAP ACPTSSAPTSHLSCTSSSTRRPGSCARGTGAAST LCSLPTTAEPAPTTPWRRST*HSCRRPRSTSDG VAEQTLQGEGRRLLLLHGRTFLTRPCPDRVRKG LPQ*GWSEAVCPLPATPKQISQWG**EG*RVCRA QGGCPWEPWKCAYVHVSAGASLCCLLGPWPLIF LLLGSWALLPWLCT*VLASSSRKIPIPGICHES LLLHPDGQQRKGHSSHGHSHPLPGGASSLGGHPS LSLGGAHALLSPLPATSHCTGGYL*PQAAFLLD TNFSLGLQASPAEAKWTPGREWVAQFWSLSFAQ PPLFC*L*GCAGLGVGTPPEWGGDSLSQ*PGLGG FSTSSGRCVTRL**TSSLGGEKSPDLLHLGSFC GVNSPSSVPCHAASITECTELRVGKRCT*SLSR HTSCLFPASPFPWLHFLAFYGVEILKSQLSLFTR ATKS*GTSVLAPRWPP*SSLGSSAIKHFIFK

Sequence NM_199265

AGAGCAGCCTCGGAACCGAGACGATGCGTGCGCTCCGCGACCGAGCCGGGCTCCTCCTCTGCGTGCTGCTGCTGGCGGCGCTGCTGGAGGCGGCGCTAGG GCTCCCCGTGAAGAAGCCGCGGCTCCGCGGACCACGGCCTGGGAGCCTCACGAGGCTCGCAGAGCCCCCTTTCCAGGTGCGCGCTAGCTCGGAGAGGAGT CCCGCCGGTGCGAGGAATCTGGCGCTTTGCGGAGTGGCGGGCCAGGGCCGCTGAGGTTCAAACGGTCAGGTCTCAGCCTCCCCAGATCCTAGGCCTCTGA AGGAAGAGGAGGAGGCACCACTGCTCCCCAGAACCCACCTGCAGGCAGAGCCACACCAACATGGATGCTGGACTGTCACTGAGCCAGCAGCCATGACCCC AGGCAACGCCACCCCTCCCAGGACCCCAGAGGTTACTCCGTTGCGGCTGGAGCTGCAGAAGCTGCCGGGATTGGCCAACACAACCTTGAGTACCCCTAAC CCTGATACCCAGGCTTCAGCCTCCCCAGATCCTAGGCCTCTGAGGGAAGAGGAGGAGGCACGACTGCTCCCCAGAACCCACCTGCAGGCAGAGCTACACC AACATGGATGTTGGACTGTCACTGAGCCAGCAGCCCTGACCCCAGGGAATGCCACGCCTCCCAGGACCCAGGAGGTTACTCCCTTGCTGCTGGAGCTGCA GAAGCTGCCAGAATTGGTCCACGCAACCTTGAGTACCCCTAACCCTGATAACCAGGTGACCATCAAGGTGGTGGAGGACCCCCAGGCCGAGGTGTCGATA GACCTGTTGGCTGAGCCCAGCAATCCCCCGCCCCAGGATACCCTTAGCTGGCTGCCCGCCCTCTGGTCCTTCCTCTGGGGAGACTACAAAGGAGAGGAAA AAGACAGGGCCCCAGGGGAGAAGGGGGAGGAAAAGGAGGAAGACGAGGACTATCCTTCAGAGGATATCGAGGGTGAGGATCAAGAGGACAAAGAGGAAGA TGAGGAAGAGCAGGCGCTCTGGTTCAATGGAACTACAGACAACTGGGACCAGGGCTGGCTGGCCCCCGGGGATTGGGTCTTCAAGGATTCTGTCAGCTAC GACTATGAGCCTCAGAAGGAGTGGAGTCCCTGGTCTCCCTGCAGTGGGAACTGCAGCACTGGCAAGCAGCAGAGGACTCGGCCCTGTGGCTATGGCTGCA CTGCCACCGAGACCCGTACCTGTGACCTGCCCTCCTGTCCTGGCACTGAGGACAAGGACACCTTGGGCCTCCCCAGTGAGGAGTGGAAGCTCCTGGCCCG CAATGCTACGGACATGCATGATCAAGATGTGGACAGCTGTGAGAAGTGGCTGAACTGCAAGAGCGACTTCCTAATCAAGTATCTGAGCCAGATGCTGCGG GACCTGCCCAGCTGCCCGTGTGCCTACCCACTGGAGGCCATGGACAGCCCTGTGAGCCTACAGGACGAGCACCAGGGCCGCAGCTTCCGGTGGAGGGATG CCAGTGGCCCTCGCGAGCGCCTGGACATCTACCAGCCCACGGCGCGCTTCTGCCTGCGTTCCATGCTGTCTGGGGAGAGCAGCACACTGGCCGCCCAGCA CTGCTGCTATGACGAGGACAGCCGGCTGCTGACCCGTGGCAAGGGCGCCGGCATGCCCAACCTCATCAGCACCGACTTCTCACCTAAGCTGCACTTCAAG TTCGACACGACGCCCTGGATCCTGTGCAAGGGGGACTGGAGCCGCCTCCACGCTGTGCTCCCTCCCAACAACGGCCGAGCCTGCACCGACAACCCCCTGG AGGAGGAGTACCTAGCACAGTTGCAGGAGGCCAAGGAGTACTAGTGACGGGGTTGCTGAACAGACACTGCAGGGAGAGGGCAGGCGGCTGCTGCTGTTGC ACGGGAGAACTTTCCTCACCCGCCCCTGCCCAGACAGGGTGAGGAAAGGGCTCCCCCAGTGAGGTTGGTCCGAGGCTGTGTGCCCTCTGCCAGCGACCCC GAAGCAGATATCTCAGTGGGGTTAGTGAGAAGGTTGAAGGGTATGTAGGGCCCAGGGTGGGTGTCCCTGGGAGCCCTGGAAATGTGCATATGTGCATGTG TCTGCCGGGGCCTCCCTCTGCTGCCTGCTGGGACCCTGGCCACTCATTTTTCTCCTCCTTGGGAGCTGGGCTCTTCTGCCCTGGCTCTGCACATAAGTGT TAGCCAGCAGCTCCAGAAAAATCCCGATTCCCGGGATCTGCCACGAGTCACTCCTACTCCACCCTGATGGCCAGCAGAGGAAGGGCCACTCTTCTCATGG GCACAGCCATCCTTTGCCGGGGGGGGCATCCAGCCTGGGTGGCCACCCCTCCTTATCTCTGGGTGGTGCACATGCCCTTCTTTCCCCACTCCCTGCCACG AGCCACTGCACAGGAGGCTATCTGTAGCCCCAAGCTGCCTTTCTGTTGGACACCAACTTTAGTCTTGGGCTGCAAGCCAGCCCAGCTGAGGCGAAGTGGA CTCCAGGCAGGGAATGGGTTGCCCAATTCTGGTCCCTTTCCTTTGCTCAGCCCCCTCTGTTCTGCTGATTGTAGGGATGTGCAGGGCTGGGAGTTGGCAC TCCCCCCGAGTGGGGAGGTGACAGCTTGTCACAGTAGCCAGGCTTGGGTGGGTTCAGCACTAGCTCGGGACGGTGTGTCACACGTCTATAGTAAACCAGT TCTCTGGGAGGGGAAAAAAGCCCTGATTTATTGCATTTGGGCAGCTTCTGTGGTGTAAATTCTCCCAGCAGTGTCCCATGTCATGCTGCCAGCATCACTG AATGCACTGAACTCAGAGTTGGGAAGAGATGCACATAATCGCTCTCCCGGCACACCTCATGCCTCTTCCCTGCCTCCCCATTCCCCTGGCTGCACTTCCT TGCCTTCTATGGGGTTGAAATATTGAAGTCTCAACTGTCTCTGTTCACAAGAGCCACCAAAAGTTAGGGGACTTCAGTCCTAGCCCCCAGATGGCCGCCC TGAAGCTCTCTGGGCTCCTCAGCAATAAAGCACTTTATTTTCAAA

refseq_THSD3.F3 (forward primer)
refseq_THSD3.R3 (reverse primer)
Single exon skipping

Frame 0

RAASEPRRCVRSATEPGSSSACCCWRRCWRRR* GSP*RSRGSADHGLGASRGSQSPLSRCALARRGV PPVRGIWRFAEWRARAAEVQTVRSQPPQILGL* RKRRRHHCSPEPTCRQSHTNMDAGLSLSQQP*P QATPPLPGPQRLLRCGWSCRSCRDWPTQP*VPLT LIPRLQPPQILGL*GKRRRHDCSPEPTCRQSYT NMDVGLSLSQQP*PQGMPRLPGPRRLLPCCWSC RSCQNWSTQP*VPLTLITR*PSRWWRTPRPRCR* TCWLSPAIPRPRIPLAGCPPSGPSSGETTKERK KTGPQGRRGRKRRKTRTILQRISRVRIKRTKRK MRKSRRSGSMELQTTGTRAGWPPGIGSSRILSAT TMSLRRSGVPGLPAVGTAALASSRGLGPVAMAA LPPRPVPVTCPPVLALRTRTPWASPVRSGSSWP AMLRTCMIKMWTAVRSG*TARATS*SSI*ARCCG TCPAARVPTHWRPWTAL*AYRTSTRAAASGGGM PVALASAWTSTSPRRASACVPCCLGRAAHWPPS TAAMTRTAGC*PVARAPACPTSSAPTSHLSCTSS STRRPGSCARGTGAASTLCSLPTTAEPAPTTPW RRST*HSCRRPRSTSDGVAEQTLQGEGRRLLLL HGRTFLTRPCPDRVRKGLPQ*GWSEAVCPLPATP KQISQWG**EG*RVCRAQGGCPWEPWKCAYVHV SAGASLCCLLGPWPLIFLLLGSWALLPWLCT*V LASSSRKIPIPGICHESLLLHPDGQQRKGHSSHG HSHPLPGGASSLGGHPSLSLGGAHALLSPLPAT SHCTGGYL*PQAAFLLDTNFSLGLQASPAEAKW TPGREWVAQFWSLSFAQPPLFC*L*GCAGLGVGT PPEWGGDSLSQ*PGLGGFSTSSGRCVTRL**TS SLGGEKSPDLLHLGSFCGVNSPSSVPCHAASIT ECTELRVGKRCT*SLSRHTSCLFPASPFPWLHFL AFYGVEILKSQLSLFTRATKS*GTSVLAPRWPP *SSLGSSAIKHFIFK

Frame 1

EQPRNRDDACAPRPSRAPPLRAAAGGAAGGGAR APREEAAAPRTTAWEPHEARRAPFPGAR*LGEE SRRCEESGALRSGGPGPLRFKRSGLSLPRS*ASE GRGGGTTAPQNPPAGRATPTWMLDCH*ASSHDP RQRHPSQDPRGYSVAAGAAEAAGIGQHNLEYP* P*YPGFSLPRS*ASEGRGGGTTAPQNPPAGRATP TWMLDCH*ASSPDPRECHASQDPGGYSLAAGAA EAARIGPRNLEYP*P**PGDHQGGGGPPGRGVD RPVG*AQQSPAPGYP*LAARPLVLPLGRLQRRGK RQGPRGEGGGKGGRRGLSFRGYRG*GSRGQRGR *GRAGALVQWNYRQLGPGLAGPRGLGLQGFCQL RL*ASEGVESLVSLQWELQHWQAAEDSALWLWLH CHRDPYL*PALLSWH*GQGHLGPPQ*GVEAPGP QCYGHA*SRCGQL*EVAELQERLPNQVSEPDAA GPAQLPVCLPTGGHGQPCEPTGRAPGPQLPVEGC QWPSRAPGHLPAHGALLPAFHAVWGEQHTGRPA LLL*RGQPAADPWQGRRHAQPHQHRLLT*AALQ VRHDALDPVQGGLEPPPRCAPSQQRPSLHRQPPG GGVPSTVAGGQGVLVTGLLNRHCRERAGGCCCC TGELSSPAPAQTG*GKGSPSEVGPRLCALCQRP RSRYLSGVSEKVEGYVGPRVGVPGSPGNVHMCMC LPGPPSAACWDPGHSFFSSLGAGLFCPGSAHKC *PAAPEKSRFPGSATSHSYSTLMASRGRATLLM GTAILCRGGHPAWVATPPYLWVVHMPFFPHSLPR ATAQEAICSPKLPFCWTPTLVLGCKPAQLRRSG LQAGNGLPNSGPFPLLSPLCSADCRDVQGWELA LPPSGEVTACHSSQAWVGSALARDGVSHVYSKPV LWEGKKALIYCIWAASVV*ILPAVSHVMLPASL NALNSELGRDAHNRSPGTPHASSLPPHSPGCTS LPSMGLKY*SLNCLCSQEPPKVRGLQS*PPDGRP EALWAPQQ*STLFS

Frame 2

SSLGTETMRALRDRAGLLLCVLLLAALLEAALG LPVKKPRLRGPRPGSLTRLAEPPFQVRASSERS PAGARNLALCGVAGQGR*GSNGQVSASPDPRPL KEEEEAPLLPRTHLQAEPHQHGCWTVTEPAAMTP GNATPPRTPEVTPLRLELQKLPGLANTTLSTPN PDTQASASPDPRPLREEEEARLLPRTHLQAELH QHGCWTVTEPAALTPGNATPPRTQEVTPLLLELQ KLPELVHATLSTPNPDNQVTIKVVEDPQAEVSI DLLAEPSNPPPQDTLSWLPALWSFLWGDYKGEE KDRAPGEKGEEKEEDEDYPSEDIEGEDQEDKEED EEEQALWFNGTTDNWDQGWLAPGDWVFKDSVSY DYEPQKEWSPWSPCSGNCSTGKQQRTRPCGYGC TATETRTCDLPSCPGTEDKDTLGLPSEEWKLLAR NATDMHDQDVDSCEKWLNCKSDFLIKYLSQMLR DLPSCPCAYPLEAMDSPVSLQDEHQGRSFRWRD ASGPRERLDIYQPTARFCLRSMLSGESSTLAAQH CCYDEDSRLLTRGKGAGMPNLISTDFSPKLHFK FDTTPWILCKGDWSRLHAVLPPNNGRACTDNPL EEEYLAQLQEAKEY**RGC*TDTAGRGQAAAAVA RENFPHPPLPRQGEERAPPVRLVRGCVPSASDP EADISVGLVRRLKGM*GPGWVSLGALEMCICAC VCRGLPLLPAGTLATHFSPPWELGSSALALHISV SQQLQKNPDSRDLPRVTPTPP*WPAEEGPLFSW AQPSFAGGGIQPGWPPLLISGWCTCPSFPTPCH EPLHRRLSVAPSCLSVGHQL*SWAASQPS*GEVD SRQGMGCPILVPFLCSAPSVLLIVGMCRAGSWH SPRVGR*QLVTVARLGWVQH*LGTVCHTSIVNQ FSGRGKKP*FIAFGQLLWCKFSQQCPMSCCQHH* MH*TQSWEEMHIIALPAHLMPLPCLPIPLAALP CLLWG*NIEVSTVSVHKSHQKLGDFSPSPQMAA LKLSGLLSNKALYFQ

Sequence NM_199296

AGAGCAGCCTCGGAACCGAGACGATGCGTGCGCTCCGCGACCGAGCCGGGCTCCTCCTCTGCGTGCTGCTGCTGGCGGCGCTGCTGGAGGCGGCGCTAGG GCTCCCCGTGAAGAAGCCGCGGCTCCGCGGACCACGGCCTGGGAGCCTCACGAGGCTCGCAGAGGTCTCAGCCTCCCCAGATCCTAGGCCTCTGAAGGAA GAGGAGGAGGCACCACTGCTCCCCAGAACCCACCTGCAGGCAGAGCCACACCAACATGGATGCTGGACTGTCACTGAGCCAGCAGCCATGACCCCAGGCA ACGCCACCCCTCCCAGGACCCCAGAGGTTACTCCGTTGCGGCTGGAGCTGCAGAAGCTGCCGGGATTGGCCAACACAACCTTGAGTACCCCTAACCCTGA TACCCAGGCTTCAGCCTCCCCAGATCCTAGGCCTCTGAGGGAAGAGGAGGAGGCACGACTGCTCCCCAGAACCCACCTGCAGGCAGAGCTACACCAACAT GGATGTTGGACTGTCACTGAGCCAGCAGCCCTGACCCCAGGGAATGCCACGCCTCCCAGGACCCAGGAGGTTACTCCCTTGCTGCTGGAGCTGCAGAAGC TGCCAGAATTGGTCCACGCAACCTTGAGTACCCCTAACCCTGATAACCAGGTGACCATCAAGGTGGTGGAGGACCCCCAGGCCGAGGTGTCGATAGACCT GTTGGCTGAGCCCAGCAATCCCCCGCCCCAGGATACCCTTAGCTGGCTGCCCGCCCTCTGGTCCTTCCTCTGGGGAGACTACAAAGGAGAGGAAAAAGAC AGGGCCCCAGGGGAGAAGGGGGAGGAAAAGGAGGAAGACGAGGACTATCCTTCAGAGGATATCGAGGGTGAGGATCAAGAGGACAAAGAGGAAGATGAGG AAGAGCAGGCGCTCTGGTTCAATGGAACTACAGACAACTGGGACCAGGGCTGGCTGGCCCCCGGGGATTGGGTCTTCAAGGATTCTGTCAGCTACGACTA TGAGCCTCAGAAGGAGTGGAGTCCCTGGTCTCCCTGCAGTGGGAACTGCAGCACTGGCAAGCAGCAGAGGACTCGGCCCTGTGGCTATGGCTGCACTGCC ACCGAGACCCGTACCTGTGACCTGCCCTCCTGTCCTGGCACTGAGGACAAGGACACCTTGGGCCTCCCCAGTGAGGAGTGGAAGCTCCTGGCCCGCAATG CTACGGACATGCATGATCAAGATGTGGACAGCTGTGAGAAGTGGCTGAACTGCAAGAGCGACTTCCTAATCAAGTATCTGAGCCAGATGCTGCGGGACCT GCCCAGCTGCCCGTGTGCCTACCCACTGGAGGCCATGGACAGCCCTGTGAGCCTACAGGACGAGCACCAGGGCCGCAGCTTCCGGTGGAGGGATGCCAGT GGCCCTCGCGAGCGCCTGGACATCTACCAGCCCACGGCGCGCTTCTGCCTGCGTTCCATGCTGTCTGGGGAGAGCAGCACACTGGCCGCCCAGCACTGCT GCTATGACGAGGACAGCCGGCTGCTGACCCGTGGCAAGGGCGCCGGCATGCCCAACCTCATCAGCACCGACTTCTCACCTAAGCTGCACTTCAAGTTCGA CACGACGCCCTGGATCCTGTGCAAGGGGGACTGGAGCCGCCTCCACGCTGTGCTCCCTCCCAACAACGGCCGAGCCTGCACCGACAACCCCCTGGAGGAG GAGTACCTAGCACAGTTGCAGGAGGCCAAGGAGTACTAGTGACGGGGTTGCTGAACAGACACTGCAGGGAGAGGGCAGGCGGCTGCTGCTGTTGCACGGG AGAACTTTCCTCACCCGCCCCTGCCCAGACAGGGTGAGGAAAGGGCTCCCCCAGTGAGGTTGGTCCGAGGCTGTGTGCCCTCTGCCAGCGACCCCGAAGC AGATATCTCAGTGGGGTTAGTGAGAAGGTTGAAGGGTATGTAGGGCCCAGGGTGGGTGTCCCTGGGAGCCCTGGAAATGTGCATATGTGCATGTGTCTGC CGGGGCCTCCCTCTGCTGCCTGCTGGGACCCTGGCCACTCATTTTTCTCCTCCTTGGGAGCTGGGCTCTTCTGCCCTGGCTCTGCACATAAGTGTTAGCC AGCAGCTCCAGAAAAATCCCGATTCCCGGGATCTGCCACGAGTCACTCCTACTCCACCCTGATGGCCAGCAGAGGAAGGGCCACTCTTCTCATGGGCACA GCCATCCTTTGCCGGGGGGGGCATCCAGCCTGGGTGGCCACCCCTCCTTATCTCTGGGTGGTGCACATGCCCTTCTTTCCCCACTCCCTGCCACGAGCCA CTGCACAGGAGGCTATCTGTAGCCCCAAGCTGCCTTTCTGTTGGACACCAACTTTAGTCTTGGGCTGCAAGCCAGCCCAGCTGAGGCGAAGTGGACTCCA GGCAGGGAATGGGTTGCCCAATTCTGGTCCCTTTCCTTTGCTCAGCCCCCTCTGTTCTGCTGATTGTAGGGATGTGCAGGGCTGGGAGTTGGCACTCCCC CCGAGTGGGGAGGTGACAGCTTGTCACAGTAGCCAGGCTTGGGTGGGTTCAGCACTAGCTCGGGACGGTGTGTCACACGTCTATAGTAAACCAGTTCTCT GGGAGGGGAAAAAAGCCCTGATTTATTGCATTTGGGCAGCTTCTGTGGTGTAAATTCTCCCAGCAGTGTCCCATGTCATGCTGCCAGCATCACTGAATGC ACTGAACTCAGAGTTGGGAAGAGATGCACATAATCGCTCTCCCGGCACACCTCATGCCTCTTCCCTGCCTCCCCATTCCCCTGGCTGCACTTCCTTGCCT TCTATGGGGTTGAAATATTGAAGTCTCAACTGTCTCTGTTCACAAGAGCCACCAAAAGTTAGGGGACTTCAGTCCTAGCCCCCAGATGGCCGCCCTGAAG CTCTCTGGGCTCCTCAGCAATAAAGCACTTTATTTTCAAA

refseq_THSD3.F3 (forward primer)
refseq_THSD3.R3 (reverse primer)
Single exon skipping

Frame 0

RAASEPRRCVRSATEPGSSSACCCWRRCWRRR* GSP*RSRGSADHGLGASRGSQRSQPPQILGL*RK RRRHHCSPEPTCRQSHTNMDAGLSLSQQP*PQA TPPLPGPQRLLRCGWSCRSCRDWPTQP*VPLTL IPRLQPPQILGL*GKRRRHDCSPEPTCRQSYTNM DVGLSLSQQP*PQGMPRLPGPRRLLPCCWSCRS CQNWSTQP*VPLTLITR*PSRWWRTPRPRCR*T CWLSPAIPRPRIPLAGCPPSGPSSGETTKERKKT GPQGRRGRKRRKTRTILQRISRVRIKRTKRKMR KSRRSGSMELQTTGTRAGWPPGIGSSRILSATT MSLRRSGVPGLPAVGTAALASSRGLGPVAMAALP PRPVPVTCPPVLALRTRTPWASPVRSGSSWPAM LRTCMIKMWTAVRSG*TARATS*SSI*ARCCGT CPAARVPTHWRPWTAL*AYRTSTRAAASGGGMPV ALASAWTSTSPRRASACVPCCLGRAAHWPPSTA AMTRTAGC*PVARAPACPTSSAPTSHLSCTSSS TRRPGSCARGTGAASTLCSLPTTAEPAPTTPWRR ST*HSCRRPRSTSDGVAEQTLQGEGRRLLLLHG RTFLTRPCPDRVRKGLPQ*GWSEAVCPLPATPK QISQWG**EG*RVCRAQGGCPWEPWKCAYVHVSA GASLCCLLGPWPLIFLLLGSWALLPWLCT*VLA SSSRKIPIPGICHESLLLHPDGQQRKGHSSHGH SHPLPGGASSLGGHPSLSLGGAHALLSPLPATSH CTGGYL*PQAAFLLDTNFSLGLQASPAEAKWTP GREWVAQFWSLSFAQPPLFC*L*GCAGLGVGTP PEWGGDSLSQ*PGLGGFSTSSGRCVTRL**TSSL GGEKSPDLLHLGSFCGVNSPSSVPCHAASITEC TELRVGKRCT*SLSRHTSCLFPASPFPWLHFLA FYGVEILKSQLSLFTRATKS*GTSVLAPRWPP*S SLGSSAIKHFIFK

Frame 1

EQPRNRDDACAPRPSRAPPLRAAAGGAAGGGAR APREEAAAPRTTAWEPHEARRGLSLPRS*ASEG RGGGTTAPQNPPAGRATPTWMLDCH*ASSHDPRQ RHPSQDPRGYSVAAGAAEAAGIGQHNLEYP*P* YPGFSLPRS*ASEGRGGGTTAPQNPPAGRATPT WMLDCH*ASSPDPRECHASQDPGGYSLAAGAAEA ARIGPRNLEYP*P**PGDHQGGGGPPGRGVDRP VG*AQQSPAPGYP*LAARPLVLPLGRLQRRGKR QGPRGEGGGKGGRRGLSFRGYRG*GSRGQRGR*G RAGALVQWNYRQLGPGLAGPRGLGLQGFCQLRL *ASEGVESLVSLQWELQHWQAAEDSALWLWLHC HRDPYL*PALLSWH*GQGHLGPPQ*GVEAPGPQC YGHA*SRCGQL*EVAELQERLPNQVSEPDAAGP AQLPVCLPTGGHGQPCEPTGRAPGPQLPVEGCQ WPSRAPGHLPAHGALLPAFHAVWGEQHTGRPALL L*RGQPAADPWQGRRHAQPHQHRLLT*AALQVR HDALDPVQGGLEPPPRCAPSQQRPSLHRQPPGG GVPSTVAGGQGVLVTGLLNRHCRERAGGCCCCTG ELSSPAPAQTG*GKGSPSEVGPRLCALCQRPRS RYLSGVSEKVEGYVGPRVGVPGSPGNVHMCMCL PGPPSAACWDPGHSFFSSLGAGLFCPGSAHKC*P AAPEKSRFPGSATSHSYSTLMASRGRATLLMGT AILCRGGHPAWVATPPYLWVVHMPFFPHSLPRA TAQEAICSPKLPFCWTPTLVLGCKPAQLRRSGLQ AGNGLPNSGPFPLLSPLCSADCRDVQGWELALP PSGEVTACHSSQAWVGSALARDGVSHVYSKPVL WEGKKALIYCIWAASVV*ILPAVSHVMLPASLNA LNSELGRDAHNRSPGTPHASSLPPHSPGCTSLP SMGLKY*SLNCLCSQEPPKVRGLQS*PPDGRPE ALWAPQQ*STLFS

Frame 2

SSLGTETMRALRDRAGLLLCVLLLAALLEAALG LPVKKPRLRGPRPGSLTRLAEVSASPDPRPLKE EEEAPLLPRTHLQAEPHQHGCWTVTEPAAMTPG NATPPRTPEVTPLRLELQKLPGLANTTLSTPNPD TQASASPDPRPLREEEEARLLPRTHLQAELHQH GCWTVTEPAALTPGNATPPRTQEVTPLLLELQK LPELVHATLSTPNPDNQVTIKVVEDPQAEVSIDL LAEPSNPPPQDTLSWLPALWSFLWGDYKGEEKD RAPGEKGEEKEEDEDYPSEDIEGEDQEDKEEDE EEQALWFNGTTDNWDQGWLAPGDWVFKDSVSYDY EPQKEWSPWSPCSGNCSTGKQQRTRPCGYGCTA TETRTCDLPSCPGTEDKDTLGLPSEEWKLLARN ATDMHDQDVDSCEKWLNCKSDFLIKYLSQMLRDL PSCPCAYPLEAMDSPVSLQDEHQGRSFRWRDAS GPRERLDIYQPTARFCLRSMLSGESSTLAAQHC CYDEDSRLLTRGKGAGMPNLISTDFSPKLHFKFD TTPWILCKGDWSRLHAVLPPNNGRACTDNPLEE EYLAQLQEAKEY**RGC*TDTAGRGQAAAAVAR ENFPHPPLPRQGEERAPPVRLVRGCVPSASDPEA DISVGLVRRLKGM*GPGWVSLGALEMCICACVC RGLPLLPAGTLATHFSPPWELGSSALALHISVS QQLQKNPDSRDLPRVTPTPP*WPAEEGPLFSWAQ PSFAGGGIQPGWPPLLISGWCTCPSFPTPCHEP LHRRLSVAPSCLSVGHQL*SWAASQPS*GEVDS RQGMGCPILVPFLCSAPSVLLIVGMCRAGSWHSP RVGR*QLVTVARLGWVQH*LGTVCHTSIVNQFS GRGKKP*FIAFGQLLWCKFSQQCPMSCCQHH*M H*TQSWEEMHIIALPAHLMPLPCLPIPLAALPCL LWG*NIEVSTVSVHKSHQKLGDFSPSPQMAALK LSGLLSNKALYFQ