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Sequences

Sequence NM_182666

GGAAGCCATTGCCTGTTTAATAGTTGCTGTTGCTGCACTTCCGCTTCTCTCCCAGCGAGAGAGAGACACGAGTGGCCAGGCCCAGCCGCAGCCGCAGCAG
CAGCCGCCGCGGCGGCACGGAGGAGCCAGACACAAAGAGAGGGGCTGTTTGCGGGGTGGGGTGGGGGGTTCGCTATGTCGGATGACGATTCGAGGGCCAG
CACCAGCTCCTCCTCATCTTCGTCTTCCAACCAGCAAACCGAGAAAGAAACAAACACCCCCAAGAAGAAGGAGAGTAAAGTCAGCATGAGCAAAAACTCC
AAACTCCTCTCCACCAGCGCCAAGAGTGCTGGTCCCAAAGGCGATAACATCTATGAATGGAGATCAACCATTCTAGGGCCTCCAGGATCCGTGTATGAGG
GTGGTGTATTCTTTCTCGATATCACTTTTACACCAGAATATCCCTTCAAGCCTCCAAAGGTTACATTTCGGACAAGAATCTATCATTGTAATATTAACAG
TCAAGGTGTTATTTGCTTGGACATATTGAAAGATAATTGGAGTCCAGCACTAACCATTTCTAAAGTCCTCCTTTCTATCTGCTCACTTCTTACAGACTGT
AATCCTGCCGACCCCTTGGTGGGAAGTATTGCCACTCAGTATATGACCAACAGAGCAGAACATGACAGAATGGCCAGACAGTGGACCAAGAGATACGCTA
CATAAATTGGGGTTTCACAATTCTTACATTATTTGTCTGTCACAGAAGAGAGCTGCTTATGATTTTGAAGGGGTCAGGGAGGGTGGGAGTTGGTAAAGAG
TAGGGTATTTCTATAACAGATATTATTCAGTCTTATTTCCTAAGATTTTGTTGTAACTTAAGGTATCTTGCTACAGTAGACAGAATTGGTAATAGCAACT
TTTAAAATTGTCATTAGTTCTGCAATATTAGCTGAAATGTAGTACAGAAAAGAATGTACATTTAGACATTTGGGTTCAGTTGCTTGTAGTCTGTAAATTT
AAAACAGCTTAATTTGGTACAGGTTACACATATGGCCATTTATGTAAAGTCCCTCTAAGACTACATACTTTTTGTTTAAAACAAAATTGGAATTTGTTTT
CCCTTCTTGGAAGGGAACATTGATATTTAACAGAGTTTTTAGAGATTGTCATCTCATATATATAAAATGGACACGTGGCTATAAAACACCATATAAGAGA
TGAGTAGTGCGTTTTATTTTATATGCCAATCTACTTTGTTTAAAAAAGGTCTGAATCAGGACTTGTGAAAACCTGTAGTGAAATACCTTAAGCTGTTAAC
TAACTGTAAGGCGTGGAATAGGAGTTGCTCAGTGGATTGGTTCTATGTTGTGGACTACTTAAGTCTGCATTTGTTACTGTGCTAATAAACAATATTAAAA
ACCACCTAATAAACA
  • refseq_UBE2E1.F1 (forward primer)
  • refseq_UBE2E1.R1 (reverse primer)
  • Single exon skipping

Frame 0

GSHCLFNSCCCCTSASLPARERHEWPGPAAAAA
AAAAAARRSQTQREGLFAGWGGGFAMSDDDSRAS
TSSSSSSSSNQQTEKETNTPKKKESKVSMSKNS
KLLSTSAKSAGPKGDNIYEWRSTILGPPGSVYE
GGVFFLDITFTPEYPFKPPKVTFRTRIYHCNINS
QGVICLDILKDNWSPALTISKVLLSICSLLTDC
NPADPLVGSIATQYMTNRAEHDRMARQWTKRYA
T*IGVSQFLHYLSVTEESCL*F*RGQGGWELVKS
RVFL*QILFSLIS*DFVVT*GILLQ*TELVIAT
FKIVISSAILAEM*YRKECTFRHLGSVACSL*I
*NSLIWYRLHIWPFM*SPSKTTYFLFKTKLEFVF
PSWKGTLIFNRVFRDCHLIYIKWTRGYKTPYKR
*VVRFILYANLLCLKKV*IRTCENL**NTLSC*
LTVRRGIGVAQWIGSMLWTT*VCICYCANKQY*K
PPNK

Frame 1

EAIACLIVAVAALPLLSQRERDTSGQAQPQPQQ
QPPRRHGGARHKERGCLRGGVGGSLCRMTIRGP
APAPPHLRLPTSKPRKKQTPPRRRRVKSA*AKTP
NSSPPAPRVLVPKAITSMNGDQPF*GLQDPCMR
VVYSFSISLLHQNIPSSLQRLHFGQESIIVILT
VKVLFAWTY*KIIGVQH*PFLKSSFLSAHFLQTV
ILPTPWWEVLPLSI*PTEQNMTEWPDSGPRDTL
HKLGFHNSYIICLSQKRAAYDFEGVREGGSW*R
VGYFYNRYYSVLFPKILL*LKVSCYSRQNW**QL
LKLSLVLQY*LKCSTEKNVHLDIWVQLLVVCKF
KTA*FGTGYTYGHLCKVPLRLHTFCLKQNWNLF
SLLGREH*YLTEFLEIVISYI*NGHVAIKHHIRD
E*CVLFYMPIYFV*KRSESGLVKTCSEIP*AVN
*L*GVE*ELLSGLVLCCGLLKSAFVTVLINNIK
NHLIN

Frame 2

KPLPV**LLLLHFRFSPSERETRVARPSRSRSS
SRRGGTEEPDTKRGAVCGVGWGVRYVG*RFEGQ
HQLLLIFVFQPANRERNKHPQEEGE*SQHEQKL
QTPLHQRQECWSQRR*HL*MEINHSRASRIRV*G
WCILSRYHFYTRISLQASKGYISDKNLSL*Y*Q
SRCYLLGHIER*LESSTNHF*SPPFYLLTSYRL
*SCRPLGGKYCHSVYDQQSRT*QNGQTVDQEIRY
INWGFTILTLFVCHRRELLMILKGSGRVGVGKE
*GISITDIIQSYFLRFCCNLRYLATVDRIGNSN
F*NCH*FCNIS*NVVQKRMYI*TFGFSCL*SVNL
KQLNLVQVTHMAIYVKSL*DYILFV*NKIGICF
PFLEGNIDI*QSF*RLSSHIYKMDTWL*NTI*E
MSSAFYFICQSTLFKKGLNQDL*KPVVKYLKLLT
NCKAWNRSCSVDWFYVVDYLSLHLLLC**TILK
TT**T

Sequence NM_003341

GGAAGCCATTGCCTGTTTAATAGTTGCTGTTGCTGCACTTCCGCTTCTCTCCCAGCGAGAGAGAGACACGAGTGGCCAGGCCCAGCCGCAGCCGCAGCAG
CAGCCGCCGCGGCGGCACGGAGGAGCCAGACACAAAGAGAGGGGCTGTTTGCGGGGTGGGGTGGGGGGTTCGCTATGTCGGATGACGATTCGAGGGCCAG
CACCAGCTCCTCCTCATCTTCGTCTTCCAACCAGCAAACCGAGAAAGAAACAAACACCCCCAAGAAGAAGGAGAGTAAAGTCAGCATGAGCAAAAACTCC
AAACTCCTCTCCACCAGCGCCAAGAGAATTCAGAAGGAGCTGGCGGACATCACTTTAGACCCTCCACCTAATTGCAGTGCTGGTCCCAAAGGCGATAACA
TCTATGAATGGAG
ATCAACCATTCTAGGGCCTCCAGGATCCGTGTATGAGGGTGGTGTATTCTTTCTCGATATCACTTTTACACCAGAATATCCCTTCAA
GCCTCCAAAGGTTACATTTCGGACAAGAATCTATCATTGTAATATTAACAGTCAAGGTGTTATTTGCTTGGACATATTGAAAGATAATTGGAGTCCAGCA
CTAACCATTTCTAAAGTCCTCCTTTCTATCTGCTCACTTCTTACAGACTGTAATCCTGCCGACCCCTTGGTGGGAAGTATTGCCACTCAGTATATGACCA
ACAGAGCAGAACATGACAGAATGGCCAGACAGTGGACCAAGAGATACGCTACATAAATTGGGGTTTCACAATTCTTACATTATTTGTCTGTCACAGAAGA
GAGCTGCTTATGATTTTGAAGGGGTCAGGGAGGGTGGGAGTTGGTAAAGAGTAGGGTATTTCTATAACAGATATTATTCAGTCTTATTTCCTAAGATTTT
GTTGTAACTTAAGGTATCTTGCTACAGTAGACAGAATTGGTAATAGCAACTTTTAAAATTGTCATTAGTTCTGCAATATTAGCTGAAATGTAGTACAGAA
AAGAATGTACATTTAGACATTTGGGTTCAGTTGCTTGTAGTCTGTAAATTTAAAACAGCTTAATTTGGTACAGGTTACACATATGGCCATTTATGTAAAG
TCCCTCTAAGACTACATACTTTTTGTTTAAAACAAAATTGGAATTTGTTTTCCCTTCTTGGAAGGGAACATTGATATTTAACAGAGTTTTTAGAGATTGT
CATCTCATATATATAAAATGGACACGTGGCTATAAAACACCATATAAGAGATGAGTAGTGCGTTTTATTTTATATGCCAATCTACTTTGTTTAAAAAAGG
TCTGAATCAGGACTTGTGAAAACCTGTAGTGAAATACCTTAAGCTGTTAACTAACTGTAAGGCGTGGAATAGGAGTTGCTCAGTGGATTGGTTCTATGTT
GTGGACTACTTAAGTCTGCATTTGTTACTGTGCTAATAAACAATATTAAAAACCACCTAATAAACA
  • refseq_UBE2E1.F1 (forward primer)
  • refseq_UBE2E1.R1 (reverse primer)
  • Single exon skipping

Frame 0

GSHCLFNSCCCCTSASLPARERHEWPGPAAAAA
AAAAAARRSQTQREGLFAGWGGGFAMSDDDSRAS
TSSSSSSSSNQQTEKETNTPKKKESKVSMSKNS
KLLSTSAKRIQKELADITLDPPPNCSAGPKGDN
IYEWR
STILGPPGSVYEGGVFFLDITFTPEYPFK
PPKVTFRTRIYHCNINSQGVICLDILKDNWSPA
LTISKVLLSICSLLTDCNPADPLVGSIATQYMT
NRAEHDRMARQWTKRYAT*IGVSQFLHYLSVTEE
SCL*F*RGQGGWELVKSRVFL*QILFSLIS*DF
VVT*GILLQ*TELVIATFKIVISSAILAEM*YR
KECTFRHLGSVACSL*I*NSLIWYRLHIWPFM*S
PSKTTYFLFKTKLEFVFPSWKGTLIFNRVFRDC
HLIYIKWTRGYKTPYKR*VVRFILYANLLCLKK
V*IRTCENL**NTLSC*LTVRRGIGVAQWIGSML
WTT*VCICYCANKQY*KPPNK

Frame 1

EAIACLIVAVAALPLLSQRERDTSGQAQPQPQQ
QPPRRHGGARHKERGCLRGGVGGSLCRMTIRGP
APAPPHLRLPTSKPRKKQTPPRRRRVKSA*AKTP
NSSPPAPREFRRSWRTSL*TLHLIAVLVPKAIT
SMNG
DQPF*GLQDPCMRVVYSFSISLLHQNIPS
SLQRLHFGQESIIVILTVKVLFAWTY*KIIGVQH
*PFLKSSFLSAHFLQTVILPTPWWEVLPLSI*P
TEQNMTEWPDSGPRDTLHKLGFHNSYIICLSQK
RAAYDFEGVREGGSW*RVGYFYNRYYSVLFPKIL
L*LKVSCYSRQNW**QLLKLSLVLQY*LKCSTE
KNVHLDIWVQLLVVCKFKTA*FGTGYTYGHLCK
VPLRLHTFCLKQNWNLFSLLGREH*YLTEFLEIV
ISYI*NGHVAIKHHIRDE*CVLFYMPIYFV*KR
SESGLVKTCSEIP*AVN*L*GVE*ELLSGLVLC
CGLLKSAFVTVLINNIKNHLIN

Frame 2

KPLPV**LLLLHFRFSPSERETRVARPSRSRSS
SRRGGTEEPDTKRGAVCGVGWGVRYVG*RFEGQ
HQLLLIFVFQPANRERNKHPQEEGE*SQHEQKL
QTPLHQRQENSEGAGGHHFRPST*LQCWSQRR*H
L*ME
INHSRASRIRV*GWCILSRYHFYTRISLQ
ASKGYISDKNLSL*Y*QSRCYLLGHIER*LESS
TNHF*SPPFYLLTSYRL*SCRPLGGKYCHSVYDQ
QSRT*QNGQTVDQEIRYINWGFTILTLFVCHRR
ELLMILKGSGRVGVGKE*GISITDIIQSYFLRF
CCNLRYLATVDRIGNSNF*NCH*FCNIS*NVVQK
RMYI*TFGFSCL*SVNLKQLNLVQVTHMAIYVK
SL*DYILFV*NKIGICFPFLEGNIDI*QSF*RL
SSHIYKMDTWL*NTI*EMSSAFYFICQSTLFKKG
LNQDL*KPVVKYLKLLTNCKAWNRSCSVDWFYV
VDYLSLHLLLC**TILKTT**T

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