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Sequence NM_004245
CAGCTACCATGGCCCAAGGGCTAGAAGTGGCCCTCACAGACCTCCAGAGCTCCAGAAATAATGTGCGGCACCACACGGAGGAGATCACTGTGGACCACCTGCTTGTTCGCCGGGGCCAGGCCTTCAACCTCACCCTGTACTTCAGGAACCGGAGCTTCCAGCCAGGCCTGGACAACATCATCTTCGTGGTTGAAACTGAG
GATGCTGTCTACTTGGACAGTGAACCCCAGAGGCAGGAGTATGTCATGAATGATTATGGCTTCATCTACCAAGGCAGCAAGAACTGGATCCGCCCATGTC
CCTGGAACTATGGACAGTTTGAAGACAAAATCATAGACATCTGCCTGAAGCTGCTAGACAAGAGCCTGCACTTCCAGACTGACCCAGCCACAGACTGTGC
TCTGCGGGGAAGCCCCGTCTACGTCAGCAGAGTGGTGTGTGCCATGATCAACAGCAATGATGATAATGGGGTGCTCAATGGAAACTGGAGTGAGAATTAC
ACAGACGGCGCCAACCCTGCGGAGTGGACGGGCAGCGTGGCCATCCTGAAGCAGTGGAACGCCACAGGCTGCCAGCCCGTGCGCTACGGGCAATGCTGGG
TCTTTGCTGCCGTCATGTGCACAGTGATGAGGTGTCTGGGGATCCCTACCCGTGTGATCACCAACTTCGACTCTGGCCACGATACAGATGGAAACCTGAT
CATAGATGAGTATTATGACAACACAGGCAGGATTTTGGGGAATAAGAAGAAGGATACTATCTGGAACTTCCATGTCTGGAATGAGTGCTGGATGGCCCGG
AAGGATCTGCCCCCTGCATATGGAGGCTGGCAGGTGCTGGACGCCACACCTCAGGAGATGAGCAACGGCGTCTACTGCTGTGGCCCTGCCTCTGTCAGAG
CCATCAAAGAAGGAGAAGTGGACCTGAACTATGACACGCCCTTTGTGTTTTCGATGGTGAATGCTGACTGCATGTCCTGGCTCGTCCAGGGAGGGAAGGA
GCAGAAGCTTCACCAGGACACGAGTTCTGTTGGCAATTTTATCAGCACAAAGAGCATCCAGAGTGACGAGCGGGATGACATCACAGAGAACTACAAGTAT
GAAGAAGGATCCCTCCAGGAGAGGCAGGTGTTTCTGAAGGCTCTGCAGAAGCTGAAGGCTAGAAGCTTCCATGGCTCCCAAAGAGGAGCAGAGTTGCAAC
CTTCCAGGCCCACATCACTGAGCCAGGACAGCCCTCGGAGCCTGCATACACCTTCCCTTCGACCCAGTGATGTGGTGCAAGTCTCCCTGAAATTCAAGCT
GCTCGACCCGCCCAACATGGGCCAGGATATATGCTTTGTCCTGCTGGCCCTCAACATGTCCTCCCAGTTCAAGGACCTCAAAGTGAACCTGAGTGCCCAG
TCTCTGCTGCACGATGGCAGCCCCCTGTCCCCATTCTGGCAGGACACAGCGTTCATCACACTCTCTCCTAAAGAAGCAAAGACCTACCCCTGCAAAATCT
CCTATTCCCAGTACAGCCAGTACCTGTCAACAGACAAGCTGATCCGCATCAGTGCCCTGGGTGAAGAGAAAAGCAGTCCTGAGAAAATCCTGGTGAACAA
GATCATCACCTTATCTTATCCAAGCATCACGATTAATGTTCTAGGAGCAGCCGTTGTGAACCAGCCACTCTCCATACAGGTGATATTTTCAAACCCCCTC
TCGGAGCAGGTTGAGGACTGTGTGCTGACTGTGGAAGGAAGTGGCCTCTTCAAGAAACAGCAGAAAGTCTTCCTTGGAGTCCTCAAACCCCAACACCAAG
CAAGCATCATTCTGGAGACCGTCCCCTTCAAGAGTGGACAAAGGCAGATCCAAGCTAATATGAGAAGCAACAAGTTTAAGGACATTAAGGGTTACAGGAA
TGTTTATGTAGACTTTGCATTATAAATTCTGGAACAACGCGCCAGACGTGTGAATTTC
- refseq_TGM5.F1 (forward primer)
- refseq_TGM5.R1 (reverse primer)
- Single exon skipping
Frame 0
QLPWPKG*KWPSQTSRAPEIMCGTTRRRSLWTTCLFAGARPSTSPCTSGTGASSQAWTTSSSWLKLR
MLSTWTVNPRGRSMS*MIMASSTKAARTGSAHV
PGTMDSLKTKS*TSA*SC*TRACTSRLTQPQTV
LCGEAPSTSAEWCVP*STAMMIMGCSMETGVRIT
QTAPTLRSGRAAWPS*SSGTPQAASPCATGNAG
SLLPSCAQ**GVWGSLPV*SPTSTLATIQMET*
S*MSIMTTQAGFWGIRRRILSGTSMSGMSAGWPG
RICPLHMEAGRCWTPHLRR*ATASTAVALPLSE
PSKKEKWT*TMTRPLCFRW*MLTACPGSSREGR
SRSFTRTRVLLAILSAQRASRVTSGMTSQRTTSM
KKDPSRRGRCF*RLCRS*RLEASMAPKEEQSCN
LPGPHH*ARTALGACIHLPFDPVMWCKSP*NSS
CSTRPTWARIYALSCWPSTCPPSSRTSK*T*VPS
LCCTMAAPCPHSGRTQRSSHSLLKKQRPTPAKS
PIPSTASTCQQTS*SASVPWVKRKAVLRKSW*T
RSSPYLIQASRLMF*EQPL*TSHSPYR*YFQTPS
RSRLRTVC*LWKEVASSRNSRKSSLESSNPNTK
QASFWRPSPSRVDKGRSKLI*EATSLRTLRVTG
MFM*TLHYKFWNNAPDV*I
Frame 1
SYHGPRARSGPHRPPELQK*CAAPHGGDHCGPPACSPGPGLQPHPVLQEPELPARPGQHHLRG*N*
GCCLLGQ*TPEAGVCHE*LWLHLPRQQELDPPMS
LELWTV*RQNHRHLPEAARQEPALPD*PSHRLC
SAGKPRLRQQSGVCHDQQQ***WGAQWKLE*EL
HRRRQPCGVDGQRGHPEAVERHRLPARALRAMLG
LCCRHVHSDEVSGDPYPCDHQLRLWPRYRWKPD
HR*VL*QHRQDFGE*EEGYYLELPCLE*VLDGP
EGSAPCIWRLAGAGRHTSGDEQRRLLLWPCLCQS
HQRRRSGPEL*HALCVFDGEC*LHVLARPGREG
AEASPGHEFCWQFYQHKEHPE*RAG*HHRELQV
*RRIPPGEAGVSEGSAEAEG*KLPWLPKRSRVAT
FQAHITEPGQPSEPAYTFPSTQ*CGASLPEIQA
ARPAQHGPGYMLCPAGPQHVLPVQGPQSEPECP
VSAARWQPPVPILAGHSVHHTLS*RSKDLPLQNL
LFPVQPVPVNRQADPHQCPG*REKQS*ENPGEQ
DHHLILSKHHD*CSRSSRCEPATLHTGDIFKPP
LGAG*GLCADCGRKWPLQETAESLPWSPQTPTPS
KHHSGDRPLQEWTKADPS*YEKQQV*GH*GLQE
CLCRLCIINSGTTRQTCEF
Frame 2
ATMAQGLEVALTDLQSSRNNVRHHTEEITVDHLLVRRGQAFNLTLYFRNRSFQPGLDNIIFVVETE
DAVYLDSEPQRQEYVMNDYGFIYQGSKNWIRPC
PWNYGQFEDKIIDICLKLLDKSLHFQTDPATDCA
LRGSPVYVSRVVCAMINSNDDNGVLNGNWSENY
TDGANPAEWTGSVAILKQWNATGCQPVRYGQCW
VFAAVMCTVMRCLGIPTRVITNFDSGHDTDGNLI
IDEYYDNTGRILGNKKKDTIWNFHVWNECWMAR
KDLPPAYGGWQVLDATPQEMSNGVYCCGPASVR
AIKEGEVDLNYDTPFVFSMVNADCMSWLVQGGKE
QKLHQDTSSVGNFISTKSIQSDERDDITENYKY
EEGSLQERQVFLKALQKLKARSFHGSQRGAELQ
PSRPTSLSQDSPRSLHTPSLRPSDVVQVSLKFKL
LDPPNMGQDICFVLLALNMSSQFKDLKVNLSAQ
SLLHDGSPLSPFWQDTAFITLSPKEAKTYPCKI
SYSQYSQYLSTDKLIRISALGEEKSSPEKILVNK
IITLSYPSITINVLGAAVVNQPLSIQVIFSNPL
SEQVEDCVLTVEGSGLFKKQQKVFLGVLKPQHQ
ASIILETVPFKSGQRQIQANMRSNKFKDIKGYRN
VYVDFAL*ILEQRARRVNF
Sequence NM_201631
CAGCTACCATGGCCCAAGGGCTAGAAGTGGCCCTCACAGACCTCCAGAGCTCCAGAAATAATGTGCGGCACCACACGGAGGAGATCACTGTGGACCACCTGCTTGTTCGCCGGGGCCAGGCCTTCAACCTCACCCTGTACTTCAGGAACCGGAGCTTCCAGCCAGGCCTGGACAACATCATCTTCGTGGTTGAAACTGGA
CCGCTGCCAGACCTGGCCTTGGGGACTCGGGCTGTGTTCAGCCTGGCACGCCATCACAGCCCCAGCCCCTGGATTGCCTGGCTGGAGACCAATGGGGCCA
CCTCCACAGAGGTGAGCTTGTGCGCTCCTCCCACGGCGGCCGTGGGTCGGTACCTCTTGAAAATCCACATCGACTCCTTCCAGGGGTCTGTGACGGCCTA
CCAGCTAGGGGAGTTCATCCTGCTTTTCAATCCCTGGTGCCCAGAGGATGCTGTCTACTTGGACAGTGAACCCCAGAGGCAGGAGTATGTCATGAATGAT
TATGGCTTCATCTACCAAGGCAGCAAGAACTGGATCCGCCCATGTCCCTGGAACTATGGACAGTTTGAAGACAAAATCATAGACATCTGCCTGAAGCTGC
TAGACAAGAGCCTGCACTTCCAGACTGACCCAGCCACAGACTGTGCTCTGCGGGGAAGCCCCGTCTACGTCAGCAGAGTGGTGTGTGCCATGATCAACAG
CAATGATGATAATGGGGTGCTCAATGGAAACTGGAGTGAGAATTACACAGACGGCGCCAACCCTGCGGAGTGGACGGGCAGCGTGGCCATCCTGAAGCAG
TGGAACGCCACAGGCTGCCAGCCCGTGCGCTACGGGCAATGCTGGGTCTTTGCTGCCGTCATGTGCACAGTGATGAGGTGTCTGGGGATCCCTACCCGTG
TGATCACCAACTTCGACTCTGGCCACGATACAGATGGAAACCTGATCATAGATGAGTATTATGACAACACAGGCAGGATTTTGGGGAATAAGAAGAAGGA
TACTATCTGGAACTTCCATGTCTGGAATGAGTGCTGGATGGCCCGGAAGGATCTGCCCCCTGCATATGGAGGCTGGCAGGTGCTGGACGCCACACCTCAG
GAGATGAGCAACGGCGTCTACTGCTGTGGCCCTGCCTCTGTCAGAGCCATCAAAGAAGGAGAAGTGGACCTGAACTATGACACGCCCTTTGTGTTTTCGA
TGGTGAATGCTGACTGCATGTCCTGGCTCGTCCAGGGAGGGAAGGAGCAGAAGCTTCACCAGGACACGAGTTCTGTTGGCAATTTTATCAGCACAAAGAG
CATCCAGAGTGACGAGCGGGATGACATCACAGAGAACTACAAGTATGAAGAAGGATCCCTCCAGGAGAGGCAGGTGTTTCTGAAGGCTCTGCAGAAGCTG
AAGGCTAGAAGCTTCCATGGCTCCCAAAGAGGAGCAGAGTTGCAACCTTCCAGGCCCACATCACTGAGCCAGGACAGCCCTCGGAGCCTGCATACACCTT
CCCTTCGACCCAGTGATGTGGTGCAAGTCTCCCTGAAATTCAAGCTGCTCGACCCGCCCAACATGGGCCAGGATATATGCTTTGTCCTGCTGGCCCTCAA
CATGTCCTCCCAGTTCAAGGACCTCAAAGTGAACCTGAGTGCCCAGTCTCTGCTGCACGATGGCAGCCCCCTGTCCCCATTCTGGCAGGACACAGCGTTC
ATCACACTCTCTCCTAAAGAAGCAAAGACCTACCCCTGCAAAATCTCCTATTCCCAGTACAGCCAGTACCTGTCAACAGACAAGCTGATCCGCATCAGTG
CCCTGGGTGAAGAGAAAAGCAGTCCTGAGAAAATCCTGGTGAACAAGATCATCACCTTATCTTATCCAAGCATCACGATTAATGTTCTAGGAGCAGCCGT
TGTGAACCAGCCACTCTCCATACAGGTGATATTTTCAAACCCCCTCTCGGAGCAGGTTGAGGACTGTGTGCTGACTGTGGAAGGAAGTGGCCTCTTCAAG
AAACAGCAGAAAGTCTTCCTTGGAGTCCTCAAACCCCAACACCAAGCAAGCATCATTCTGGAGACCGTCCCCTTCAAGAGTGGACAAAGGCAGATCCAAG
CTAATATGAGAAGCAACAAGTTTAAGGACATTAAGGGTTACAGGAATGTTTATGTAGACTTTGCATTATAAATTCTGGAACAACGCGCCAGACGTGTGAA
TTTC
- refseq_TGM5.F1 (forward primer)
- refseq_TGM5.R1 (reverse primer)
- Single exon skipping
Frame 0
QLPWPKG*KWPSQTSRAPEIMCGTTRRRSLWTTCLFAGARPSTSPCTSGTGASSQAWTTSSSWLKLD
RCQTWPWGLGLCSAWHAITAPAPGLPGWRPMGP
PPQR*ACALLPRRPWVGTS*KSTSTPSRGL*RP
TS*GSSSCFSIPGAQRMLSTWTVNPRGRSMS*MI
MASSTKAARTGSAHVPGTMDSLKTKS*TSA*SC
*TRACTSRLTQPQTVLCGEAPSTSAEWCVP*ST
AMMIMGCSMETGVRITQTAPTLRSGRAAWPS*SS
GTPQAASPCATGNAGSLLPSCAQ**GVWGSLPV
*SPTSTLATIQMET*S*MSIMTTQAGFWGIRRR
ILSGTSMSGMSAGWPGRICPLHMEAGRCWTPHLR
R*ATASTAVALPLSEPSKKEKWT*TMTRPLCFR
W*MLTACPGSSREGRSRSFTRTRVLLAILSAQR
ASRVTSGMTSQRTTSMKKDPSRRGRCF*RLCRS*
RLEASMAPKEEQSCNLPGPHH*ARTALGACIHL
PFDPVMWCKSP*NSSCSTRPTWARIYALSCWPS
TCPPSSRTSK*T*VPSLCCTMAAPCPHSGRTQRS
SHSLLKKQRPTPAKSPIPSTASTCQQTS*SASV
PWVKRKAVLRKSW*TRSSPYLIQASRLMF*EQP
L*TSHSPYR*YFQTPSRSRLRTVC*LWKEVASSR
NSRKSSLESSNPNTKQASFWRPSPSRVDKGRSK
LI*EATSLRTLRVTGMFM*TLHYKFWNNAPDV*
I
Frame 1
SYHGPRARSGPHRPPELQK*CAAPHGGDHCGPPACSPGPGLQPHPVLQEPELPARPGQHHLRG*NW
TAARPGLGDSGCVQPGTPSQPQPLDCLAGDQWGH
LHRGELVRSSHGGRGSVPLENPHRLLPGVCDGL
PARGVHPAFQSLVPRGCCLLGQ*TPEAGVCHE*
LWLHLPRQQELDPPMSLELWTV*RQNHRHLPEAA
RQEPALPD*PSHRLCSAGKPRLRQQSGVCHDQQ
Q***WGAQWKLE*ELHRRRQPCGVDGQRGHPEA
VERHRLPARALRAMLGLCCRHVHSDEVSGDPYPC
DHQLRLWPRYRWKPDHR*VL*QHRQDFGE*EEG
YYLELPCLE*VLDGPEGSAPCIWRLAGAGRHTS
GDEQRRLLLWPCLCQSHQRRRSGPEL*HALCVFD
GEC*LHVLARPGREGAEASPGHEFCWQFYQHKE
HPE*RAG*HHRELQV*RRIPPGEAGVSEGSAEA
EG*KLPWLPKRSRVATFQAHITEPGQPSEPAYTF
PSTQ*CGASLPEIQAARPAQHGPGYMLCPAGPQ
HVLPVQGPQSEPECPVSAARWQPPVPILAGHSV
HHTLS*RSKDLPLQNLLFPVQPVPVNRQADPHQC
PG*REKQS*ENPGEQDHHLILSKHHD*CSRSSR
CEPATLHTGDIFKPPLGAG*GLCADCGRKWPLQ
ETAESLPWSPQTPTPSKHHSGDRPLQEWTKADPS
*YEKQQV*GH*GLQECLCRLCIINSGTTRQTCE
F
Frame 2
ATMAQGLEVALTDLQSSRNNVRHHTEEITVDHLLVRRGQAFNLTLYFRNRSFQPGLDNIIFVVETG
PLPDLALGTRAVFSLARHHSPSPWIAWLETNGA
TSTEVSLCAPPTAAVGRYLLKIHIDSFQGSVTAY
QLGEFILLFNPWCPEDAVYLDSEPQRQEYVMND
YGFIYQGSKNWIRPCPWNYGQFEDKIIDICLKL
LDKSLHFQTDPATDCALRGSPVYVSRVVCAMINS
NDDNGVLNGNWSENYTDGANPAEWTGSVAILKQ
WNATGCQPVRYGQCWVFAAVMCTVMRCLGIPTR
VITNFDSGHDTDGNLIIDEYYDNTGRILGNKKKD
TIWNFHVWNECWMARKDLPPAYGGWQVLDATPQ
EMSNGVYCCGPASVRAIKEGEVDLNYDTPFVFS
MVNADCMSWLVQGGKEQKLHQDTSSVGNFISTKS
IQSDERDDITENYKYEEGSLQERQVFLKALQKL
KARSFHGSQRGAELQPSRPTSLSQDSPRSLHTP
SLRPSDVVQVSLKFKLLDPPNMGQDICFVLLALN
MSSQFKDLKVNLSAQSLLHDGSPLSPFWQDTAF
ITLSPKEAKTYPCKISYSQYSQYLSTDKLIRIS
ALGEEKSSPEKILVNKIITLSYPSITINVLGAAV
VNQPLSIQVIFSNPLSEQVEDCVLTVEGSGLFK
KQQKVFLGVLKPQHQASIILETVPFKSGQRQIQ
ANMRSNKFKDIKGYRNVYVDFAL*ILEQRARRVN
F
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